1. Lymph node-resident dendritic cells drive T
- Author
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Carlos A, Castellanos, Xin, Ren, Steven Lomeli, Gonzalez, Hong Kun, Li, Andrew W, Schroeder, Hong-Erh, Liang, Brian J, Laidlaw, Donglei, Hu, Angel C Y, Mak, Celeste, Eng, José R, Rodríguez-Santana, Michael, LeNoir, Qi, Yan, Juan C, Celedón, Esteban G, Burchard, Scott S, Zamvil, Satoshi, Ishido, Richard M, Locksley, Jason G, Cyster, Xiaozhu, Huang, and Jeoung-Sook, Shin
- Subjects
Mice, Inbred C57BL ,Mice, Knockout ,Mice ,Th2 Cells ,Ubiquitin-Protein Ligases ,Animals ,chemical and pharmacologic phenomena ,Dendritic Cells ,Lymph Nodes ,Article - Abstract
Type 2 T helper (T(H)2) cells are protective against parasitic worm infections but also aggravate allergic inflammation. Although the role of dendritic cells (DCs) in T(H)2 cell differentiation is well established, the underlying mechanisms are largely unknown. Here, we show that DC induction of T(H)2 cells depends on membrane-associated RING-CH-1 (MARCH1) ubiquitin ligase. The pro-T(H)2 effect of MARCH1 relied on lymph node (LN)–resident DCs, which triggered T cell receptor (TCR) signaling and induced GATA-3 expression from naïve CD4(+) T cells independent of tissue-driven migratory DCs. Mice with mutations in the ubiquitin acceptor sites of MHCII and CD86, the two substrates of MARCH1, failed to develop T(H)2 cells. These findings suggest that T(H)2 cell development depends on ubiquitin-mediated clearance of antigen-presenting and costimulatory molecules by LN-resident DCs and consequent control of TCR signaling.
- Published
- 2021