Search

Your search keyword '"Michel, Gérard. P.F."' showing total 9 results
Start Over Author "Michel, Gérard. P.F."
9 results on '"Michel, Gérard. P.F."'

6. Subcomplexes from the Xcp secretion system of Pseudomonas aeruginosa

Author

Robert, Viviane, Filloux, Alain, and Michel, Gérard. P.F.

Subjects
PSEUDOMONAS aeruginosa, GRAM-negative bacteria, PSEUDOMONAS, BACTERIA
Abstract

Abstract: In Gram-negative bacteria, most of the sec-dependent exoproteins are secreted via the type II secretion system (T2SS or secreton). In Pseudomonas aeruginosa, T2SS consists of 12 Xcp proteins (XcpA and XcpP to XcpZ) organized as a multiproteic complex within the envelope. In this study, by a co-purification approach using a His-tagged XcpZ as a bait, XcpY and XcpZ were found associated together to constitute the most stable functional unit so far isolated from the P. aeruginosa secreton. This subcomplex was also found to interact with XcpR and XcpS to form a XcpRSYZ complex which was isolated under native conditions. Another component, XcpP was not found to be associated to the complex but the results suggest that it can transiently interact with the XcpYZ subcomplex in vivo. [Copyright &y& Elsevier]

Published
2005
Full Text
View/download PDF

8. Cloning and expression in Escherichia coli of a phoA gene encoding a phosphate-irrepressible alkaline phosphatase of Zymomonas mobilis

Author

Michel, Gérard P.F., Alvarez, Elisa, Guzzo, Jean, Cami, Brigitte, and Baratti, Jacques

Abstract

The Zymomonas mobilis phoA gene, encoding a phosphate-irrepressible alkaline phosphatase (ZAPase), was cloned and its expression was studied in phoA mutants of Escherichia coli. The ZAPase was recovered in the soluble fraction of E. coli. The enzyme was synthesized constitutively and its synthesis not repressed by phosphate, unlike the phoA gene of E. coli. The phoA gene of Z. mobilis was mutagenized by Mini Mu PR13 and the mutated gene crossed into Z. mobilis in order to obtain phoA mutants by reverse genetics. Although Z. mobilis mutants with Mini Mu PR13 integrated in the chromosome were obtained, none had an allele replacement for none was defective in ZAPase.

Published
1992
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results