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12016 Background: CRCI is a debilitating consequence of cancer and its treatment, including difficulties in attention, memory, and executive function. Though CRCI can develop during the course of chemotherapy, interventions targeting CRCI during chemotherapy have not been investigated. Inflammation contributes to CRCI and thus reducing inflammation may ameliorate CRCI. Using a biobehavioral approach, we investigated 2 promising interventions that reduce inflammation: exercise and low-dose ibuprofen. Methods: This is a Phase II RCT with a 2:2 factorial design. Eligible participants were patients with cancer receiving chemotherapy who self-reported cognitive difficulties. Participants were stratified by disease type (breast cancer; gastrointestinal cancer; other) and were randomized to 1 of 4 groups for 6 weeks: exercise alone (+ placebo), ibuprofen alone, exercise + ibuprofen, or placebo only. The exercise intervention, delivered by an exercise physiologist, was Exercise for Cancer Patients (EXCAP), an individually tailored, home-based prescription of walking and resistance band training. Ibuprofen/placebo was over-encapsulated for blinding; 200mg was taken 2 times per day. Participants completed 7 cognitive assessments probing attention, memory, and executive function including the Trail Making Test (TMT) and self-report (FACT-Cog) at baseline and post-intervention. ANCOVA, controlling for baseline, assessed overall Arm effects at post-intervention. Results: Of the 110 who consented to the study, 86 participants (mean age=54; 88% female; 76% breast cancer, 21% GI; 73% Stage I-III) completed baseline assessments and were randomized to one of four study arms. Ninety percent (78/86) of those completed post-intervention. Average pill compliance across all 4 groups was balanced and averaged 90.8%. Participants in the exercise and exercise + ibuprofen arms increased 2,414 and 1,073 steps respectively compared to those in placebo and ibuprofen arms increased only 464 and 412 steps respectively from pre- to post-intervention. No study-related adverse events occurred. Intent to treat ANCOVA analyses revealed a significant improvement in attention (TMT) in exercise alone compared to placebo (21.57 seconds better; p=0.003), ibuprofen alone compared to placebo (11.27 seconds better; p=0.0475), and trend for exercise + ibuprofen (7.98 seconds better; p=0.122). Those participating in both exercise arms exhibited significant improvements in the FACT-Cog Comments from Others subdomain (p

Background Androgen deprivation therapy (ADT) can decrease the physical performance (PP) of older men with prostate cancer (PC). Methods We conducted a three-arm randomized pilot study ( n =19) comparing a home-based walking and resistance intervention (EXCAP) and a technology-mediated walking and resistance intervention using Wii-Fit to a usual-care arm in men ≥70years with PC receiving ADT. The intervention lasted for 6weeks, with follow-up at 12weeks. The primary pre-specified outcome was change in Short Physical Performance Battery (SPPB) score. Mixed effects regression models were used to assess change in outcomes over time. Results Mean participant age was 70years (range: 67–93). Eight patients were randomized to the Wii-Fit arm, 6 to the EXCAP arm, and 5 to the usual-care arm. SPPB scores remained nearly constant in the usual-care arm ( β =−0.12; p =0.79), while individuals in the EXCAP arm had on average a 1.2 point increase at each follow-up ( β =1.20; 95% CI: 0.36, 2.06). The Wii-fit arm had a non-significant increase in SPPB score over time relative to usual-care ( β =0.32; 95% CI −0.43, 1.06; p =0.46). Individuals in the EXCAP arm had an increase in steps per day over time compared to the usual-care arm ( p -value=0.006); the EXCAP arm had an increase of 2720 steps (95% CI: 1313, 4128) while the usual-care arm had an increase of 97 steps (95% CI: −1140, 1333). Participants in the Wii-Fit arm had an increase of 1020 steps (95% CI: −474, 1238, p =0.710). Other outcomes (i.e., handgrip strength, lean muscle mass, and chest press repetitions) were not statistically significant. Conclusions A home-based aerobic and resistance exercise program, EXCAP, shows promise for improving PP in older men with PC on ADT.

e21608 Background: Cardiovascular disease results in significant morbidity among TCS. Exercise for Cancer Patients (EXCAP) is a self-directed, individually tailored intervention that improves cardiovascular fitness in cancer patients, yet this has not been tested in TCS. We conducted a randomized phase II feasibility study of a novel exercise intervention using a mHealth delivery platform for EXCAP. Methods: We developed mHealth-EXCAP, which integrates data from a wearable digital activity tracker (DAT) into a patient’s electronic medical record (EMR), allowing providers to monitor and adjust EXCAP prescriptions via a patient-centered virtual portal. We randomized TCS ( < 69 yr at diagnosis, any treatments, not in active or maintenance stage of exercise behavior) into 3 study arms: Arm 1 (mHealth-EXCAP), Arm 2 (EXCAP) and Arm 3 (usual care). TCS in both Arm 1 and 2 completed a 12 week EXCAP program with the goal of 12,000 daily steps and daily resistance exercises but Arm 1 used the mHealth platform. Arm 3 used a TCS care plan that promotes exercises. Results: 74 TCS were screened (32 ineligible; 23 declined), 19 consented and 14 (74%) completed study (3 withdrew; 2 lost to follow up) as of 1/31/17. Median age (yr) was 35 (Arm 1; n = 6), 48 (Arm 2; n = 4), and 48 (Arm 3; n = 4). In Arm 1, 83% TCS wore DAT and synced daily step data to EMR > 90% of days. EXCAP prescriptions were adjusted via EMR portal on average 5 times over 12 weeks for each TCS and their weekly daily step goals were met 73% of the time. The number of daily steps recorded by DAT and those synced from DAT to EMR differed by a mean of 1.5% steps (±1.8). Median baseline number of daily steps were 5,525 (Arm 1), 6,795 (Arm 2), and 5,904 (Arm 3) and increased by 5,236, 1,160, and 1,544 respective steps at 12 weeks, with only Arm 1 (50% TCS) achieving > 12,000 daily steps. At 3 months after intervention, 33% TCS in Arm 1 maintained > 12,000 daily steps. On average, TCS in Arm 1 performed resistance exercises 26 minutes a day/5 times a week vs. 28 minutes a day/4 times a week in Arm 2. Conclusions: mHealth-EXCAP is feasible to implement among TCS and may decrease sedentary behavior. Ongoing research will examine its effects on cardiovascular fitness and risk factors.

What is the central question of this study? The relationship between the thyroid system and sleep deprivation has seldom been assessed in the literature, and mounting evidence exists that sleep disturbances influence human lifestyles. The aim of this study was to investigate the hypothalamic-pituitary-thyroid axis and thyroid hormone metabolism in sleep-deprived and sleep-restricted rats. What is the main finding and its importance? Central hypothyroidism and high thyroxine (T4 ) to 3,5,3'-triiodothyronine (T3 ) activation in brown adipose tissue were observed following sleep deprivation. Sleep-restricted rats exhibited normal thyroid-stimulating hormone and T4 concentrations despite increased circulating T3 . Sleep recovery for 24 h did not normalize the high T3 concentrations, suggesting that high T3 is a powerful counterregulatory mechanism activated following sleep deprivation. Modern life has shortened sleep time, and the consequences of sleep deprivation have been examined in both human subjects and animal models. As the relationship between thyroid function and sleep deprivation has not been fully investigated, the aim of this study was to assess the hypothalamic-pituitary-thyroid axis and thyroid hormone metabolism following paradoxical sleep deprivation (PSD) and sleep restriction (SR) in rats. The effects of a 24 h rebound period were also studied. Male Wistar rats (200-250 g, n = 10 per group) were subjected to sleep deprivation via the modified multiple platform method. Rats were assigned to the following seven groups: control, PSD for 24 or 96 h, 24 or 96 h of sleep deprivation with rebound (PSD24R and PSD96R), SR for 21 days (SR21) and SR21 with rebound (SR21R). Blood samples were collected to determine the 3,5,3'-triiodothyronine (T3 ), thyroxine (T4 ) and thyroid-stimulating hormone concentrations. Brown adipose tissue iodothyronine deiodinase type 2 (D2) activity was also evaluated. Body weight gain was dramatically reduced (by ∼50-100%) in all sleep-deprived and sleep-restricted rats; rebound restored this parameter in only the PSD24R group. The serum TSH and T4 concentrations decreased, whereas T3 increased in both the PSD24 and PSD96 groups compared with control animals (P0.05). Only PSD24R and PSD96R normalized T4 and thyroid-stimulating hormone concentrations, respectively, independently of the higher circulating T3 concentrations (∼20-30%) noted in all groups compared with control animals (P0.05). Brown adipose tissue D2 activity increased in the PSD 24 and 96 h groups (∼10 times), and PSD24R was more effective than PSD96R at restoring basal brown adipose tissue D2 activity. Our data suggest that thyroid hormone metabolism adapts to sleep deprivation-induced hypothalamic-pituitary-thyroid alterations and increases T4 to T3 activation peripherally, thereby increasing circulating T3 in rats.

Based on the relevance of the renin-angiotensin system and the ongoing controversy regarding the role of the sympathetic nervous system in thyroid hormone-induced cardiac hypertrophy, the aim of the present study was to establish whether the putative difference in the degree of cardiac hypertrophy exhibited by males and females might be related to differences in the sympathetic-vagal balance and/or in the cardiac renin-angiotensin system in mice of different genders. Male and female mice (n = 117) were given 0.1 mg kg(-1) of triiodothyronine or normal saline each day for 10 days consecutively. At the end of that period, study of the heart rate variability, spectral analysis and histopathological examination were performed to assess the sympathetic-vagal balance and the diameter of cardiomyocytes. The cardiac levels of angiotensin I and II were also measured. Treatment with triiodothyronine induced a greater degree of cardiac hypertrophy in male (~73%) than in female mice (~42%). This difference was attributed to greater modulation of the sympathetic nervous system and higher levels of angiotensin I and II in male than in female mice. Our data indicate that thyroid hormone-induced cardiac hypertrophy was more intense in male mice due to the synergic effect of the sympathetic nervous system and the cardiac renin-angiotensin system.

161 Background: Cardiovascular (CV) disease results in significant morbidity among TCS. The effects of exercise on mitigating these late effects remain unknown in this population. Exercise for Cancer Patients (EXCAP) is a self-directed, individually tailored intervention that has been shown to improve CV fitness in cancer patients. We conducted a randomized phase II feasibility study of a novel electronic implementation method (mHealth) of EXCAP. Methods: We developed mHealth-EXCAP that integrates data from a wearable digital activity tracker (DAT) into a patient’s electronic medical record (EMR), allowing providers to monitor and adjust EXCAP prescriptions via a patient-centered virtual portal. We randomized TCS ( < 69 yr at diagnosis, any treatments, not in active or maintenance stage of exercise behavior) into 3 study arms: Arm 1 (mHealth-EXCAP), Arm 2 (EXCAP) and Arm 3 (usual care). Both Arm 1 and 2 completed a 12 week EXCAP program with the goal of 12,000 daily steps and daily resistance exercises and Arm 1 has integration of mHealth. Arm 3 received a TCS care plan that encourages exercises. Results: 52 TCS were screened (23 ineligible; 12 declined), 17 consented and 13 (80.9%) completed study (3 withdrew; 1 lost to follow up) as of May 31, 2016. Median age (yr) was 34 (Arm 1; n = 6), 52 (Arm 2; n = 3), and 48 (Arm 3; n = 4). In Arm 1, 83% TCS wore DAT and synced daily step data to EMR > 90% of days. Further, EXCAP prescriptions were adjusted via EMR portal on average 5 times over 12 weeks for each TCS and their weekly daily step goals were met 73% of the time. The number of daily steps recorded by DAT and those synced from DAT to EMR differed by a mean of 1.5% steps (±1.8). Median baseline number of daily steps were 5,432 (Arm 1), 6,737 (Arm 2), and 5,875 (Arm 3) and increased by 6,161, 1,742, and 1,921 respective steps, with only Arm 1 (50% TCS) achieving > 12,000 daily steps at study completion. On average, TCS in Arm 1 and 2 performed resistance exercises 15 minutes a day/2 times a week and 34 minutes a day/3 times a week, respectively. Conclusions: mHealth-EXCAP is feasible to implement among TCS and may decrease sedentary behavior. Ongoing research will examine its effects on CV risk factors and cardiopulmonary function.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-09-06T19:29:17Z No. of bitstreams: 1 2016 - Nayana Coutinho Rodrigues.pdf: 1484143 bytes, checksum: 4c035913d5385b6295f9f1f441eaa76d (MD5) Made available in DSpace on 2022-09-06T19:29:18Z (GMT). No. of bitstreams: 1 2016 - Nayana Coutinho Rodrigues.pdf: 1484143 bytes, checksum: 4c035913d5385b6295f9f1f441eaa76d (MD5) Previous issue date: 2016-12-20 CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior Modern life is shortening the sleep time and the consequences of this decreased has been studied in humans and animal models. Nevertheless, the effects of sleep deprivation in thyroid function are rarely studied. Our group pioneered in demonstrating the effects of paradoxcal sleep deprivation in the thyroid function; previous stududy related to thyroid function did full sleep deprivation. In this study, our objective was evaluating if the recent data we observed effects on thyroid function after paradoxcal sleep deprivation were due to the sleep deprivation or secondary to an hyperadrenocorticism and/or ?-adrenergic hyperstimulation. Male rats (250-300g) from UFRRJ, animal care were kept in light-dark cycles (7-19h), controlled temperature (22? ? 2?C) with food and water ad libitum. For the propranolol protocol, the animals were distributed in 6 groups: 1 control (C), with a regular sleep pattern; 2 control treated with propranolol (C+P); 3 sleep deprived for 24h (P24); 4 sleep deprived for 24h with a 24h rebound period (P24R); 5 P24 treated with propranolol (P24+P) and 6 P24R treated with propranolol (P24R+P). All animals were treated with a 30mg/Kg dosage of propranolol benzoate or water in the control groups, for 14 days. For the adrenalectomy protocol, (Ax), the animals were anesthetized e adrenalectomized, or Shan, after 10 days of surgery the animals were divided as: Sham, sleep deprived for 24h or 96 with their respective rebound periods (P24, P96, P24R and P96R) e sleep deprived Ax for 24 e 96 hours with their respective rebound periods (AxP24, Ax P96, AxP24R e AxP96 and AxP96R). All animals were euthanized on the same day; blood was collected for T3, T4, TSH e corticosterone analisys; liver(F) and thyroid(T) for type 1 deiodinase (D1), hypophysis (H), brown adipose tissue (TAM), hypothalamus (HP) for type 2deiodinase (D2) activity. The entire procedure was approved by UFRRJ Ethics Committee 003/2015. T4 decreased in both protocols, T3 had no change in the propranolol protocol, where the D1 increases at the F in all groups and sees no changes in the T; D2 increases on TAM only in P24+P and P24R+P at the HP, while in the H D2 decreased on C+P and P24+P but normalizes after the rebound period. Corticosterone decreased in all Ax e AxP24, yet on Ax96 this decrease normalized. TSH increased in Ax e and normalized in AxP24 e AxP24R when compared to Sham. We can conclude that the decrease in T4 is independent to an adrenergic hyperactivation, for neither the adrenalectomia nor the ?-adrenergic block were effective in maintaining T4 levels and sleep deprivation leads to a failure in the feedback mechanism once the diminished T4 doesnt stimulate a TSH increase and that T3 probably suffers no alteration due to the increase in T4-T3's peripheral conversion. A vida moderna vem diminuindo o tempo de sono da maioria dos homens devido ?s exig?ncias da vida moderna, os efeitos dessa diminui??o tem sido extensivamente estudados nos ?ltimos anos em homens e em modelos animais, No entanto os efeitos da priva??o de sono na fun??o tireoidiana s?o pouco estudados. O nosso grupo foi pioneiro em demonstrar os efeitos da priva??o de sono REM na fun??o tireoidiana, trabalhos anteriores relacionados ? fun??o tireoidiana faziam priva??o de sono total. Neste trabalho nosso objetivo foi avaliar se os efeitos observados anteriormente na fun??o tireoidiana ap?s priva??o de sono REM eram diretamente pela priva??o de sono ou secund?rios a um hiperadrenocorticismo e/ou hiperestimula??o ?-adren?rgica. Ratos machos (250-300g) do biot?rio da UFRRJ foram mantidos em ciclo claro-escuro (7-19h) temperatura controlada (22? ? 2?C) com comida e ?gua ad libitum; Para o protocolo do propranolol os animais foram distribu?dos em: 1 controle (C), com padr?o de sono normal; 2 controle tratado com propranolol (C+P); 3 privados de sono por 24h (P24);4 privados de sono por 24h com rebote de 24h (P24R); 5- P24 tratados com propranolol (P24+P) e, 6 P24R tratados com propranolol (P24R+P). Todos os animais foram tratados com benzoato de propranolol na dose 30mg/Kg ou com ?gua nos grupos controles por 14 dias. Para o protocolo da adrenalectomia (Ax), os animais foram anestesiados e adrenalectomizados, falso operados (Fo), ap?s 10 dias de cirurgia os animais foram distribu?dos em: Fo, Ax adrenalectomizados com padr?o de sono normal; privados de sono por 24h ou 96 com seus respectivos rebotes( P24, P96, P24R e P96R) e Ax privados de sono por 24 e 96 horas com seus respectivos rebotes (AxP24, Ax P96, AxP24R e AxP96 e AxP96R). Todos os animais foram eutanaziados no mesmo dia, sangue coletado para an?lise de T3, T4, TSH e corticosterona; f?gado(F) e tireoide(T) para an?lise da desiodase tipo 1 (D1), hip?fise(H), tecido adiposo marrom (TAM) e hipot?lamo(HP) para a atividade da desiodase tipo 2 (D2). Todo procedimento foi aprovado pelo comit? de ?tica da UFRRJ 003/2015. O T4 diminui em ambos os protocolos, o T3 n?o muda no protocolo do propranolol, onde a D1 aumenta no F em todos os grupos e n?o sofre altera??o na T, a D2 aumenta no TAM apenas em P24+P e em P24R+P no HP enquanto em H D2 diminui em C+P e P24+P e o rebote normaliza. A corticosterona diminuiu em todos os Ax e AxP24, j? no Ax96 houve uma normaliza??o dessa diminui??o. O TSH aumentou em Ax e normalizou em AxP24 e AxP24R quando comparados ao Fo. Podemos concluir que a diminui??o do T4 ? independente de uma hiperativa??o adren?rgica, pois nem a adrenalectomia nem o bloqueio ?-adren?rgico foram eficazes na manuten??o dos n?veis de T4 e que a priva??o de sono leva ? uma falha no mecanismo de feedback uma vez que o T4 diminu?do n?o estimula o aumento do TSH e que provavelmente o T3 n?o sofre altera??o devido ao aumento da convers?o perif?rica de T4-T3.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2016-10-20T18:52:30Z No. of bitstreams: 1 2016 - Joyce Mattos de Oliveira.pdf: 2259979 bytes, checksum: dca983fbb22cab52bfa67209c105ae2f (MD5) Made available in DSpace on 2016-10-20T18:52:30Z (GMT). No. of bitstreams: 1 2016 - Joyce Mattos de Oliveira.pdf: 2259979 bytes, checksum: dca983fbb22cab52bfa67209c105ae2f (MD5) Previous issue date: 2016-03-31 CAPES Modern life has diminished the sleep time for the majority of the population, and the consequences of this reduction have been studied both in humans and animal models. In spite of this, only a few studies elucidate the effect sleep deprivation has on the thyroid function, as well as studies on any role exercise might have in the prevention of such alterations. The objective of this study is to assess the protective effect of the strength exercise on the thyroid function in rats that went through paradoxical sleep deprivation for 24 and 96 hours, as well as a rebound sleep for 24 hours. For this study male and female Wistar rats were used (200-250g), submitted to sleep deprivation using the modified multiple platforms, and the isometric exercise was offered by the inverted box proposed by Lac & Cavalie (1999). The animals were distributed in 6 groups: Control (C, males n=8, females = 13); Trained (T, males n=8; females n=13), Trained, with Sleep Deprivation of the paradoxical sleep for 24 and 96 hours (respectively TPSP24 e TPSP96 males n=10; females n=13); Trained with Sleep Deprivation for 24 and 96 hours, plus a rebound sleep for 24 hours (TPSP24R e TPSP96R males n=10; females n=13). All animals went through and adaptation to the strength exercise for 5 days, enduring 5 series of 30 seconds of strength with rest periods of 25 seconds between each series. After adaptation, an extra weight was added to the animal's tail. All animals were killed on the same day and their blood was collected for analysis of T3 (ng/dL), T4 (ug/dL), e TSH (ng/mL) using the radioimmunoassay technique. Ethics committee approval was granted by number UFRRJ N?003/2015. After the statistical analysis we observed a significant body weight loss, both in females and males, and a relative loss in hypophysis weight in males from group T. On the other hand, the relative weight of the adrenal was reduced in the T group of males, and increased in both the T and TP24 groups of females. In males, seric TSH levels have risen with the exercise, normalizing after the deprivation of 24 and 96 hours, and the rebound in the PS96 group. The PSP was able to induce a raise in the T3 level in the groups TP24 and TP96 in males ? no significant alterations were observed in females. As for the seric T4 in males, there was no alteration, although in females the 24 hours PSP was able to rise those values. This study indicates a protective effect by the isometric exercise, preventing TSH and seric T4 and T3 alterations induced by deprivation of the paradoxical sleep. As such more studies are necessary to clarify the mechanisms involved in such protection A vida moderna tem diminu?do o tempo de sono da maioria da popula??o e as consequ?ncias dessa redu??o t?m sido estudadas em humanos e modelos animais. J? o papel da tire?ide na priva??o de sono associada com exerc?cios de for?a n?o est? bem estabelecido, pois n?o tem sido estudado. Este estudo, no entanto, tem como objetivo avaliar o efeito protetor do exerc?cio de for?a sobre a fun??o tireoidiana em ratos ap?s a priva??o de sono paradoxal (PSP) por 24 e 96 horas assim como o sono rebote de 24 horas. Para a realiza??o deste trabalho, foram utilizados ratos machos e f?meas Wistar (200-250g) submetidos a priva??o de sono pela metodologia das plataformas m?ltiplas modificadas e o exerc?cio isom?trico foi feito pela metodologia da caixa invertida proposta por Lac & Cavalie (1999). Os animais machos foram distribu?dos em 6 grupos: Controle (C n=8 machos; f?meas, n=13); Treinado (T=8 machos; f?meas, n=13); Treinado com Priva??o de sono paradoxal por 24 horas e 96 horas (TPSP24 e TPSP96 n=10, machos; f?meas, n=13); Treinado com Priva??o de sono paradoxal por 24 horas e 96 horas mais per?odo de sono rebote por 24 horas (TPSP24R e TPSP96R n=10, machos; f?meas, n=13). Os animais foram adaptados ao exerc?cio de for?a por 5 dias, onde era constitu?do por 5 s?ries de 30 segundos de for?a com intervalos de descanso por 25 segundos entre as s?ries. Ap?s a adapta??o, foi adicionado um peso extra na cauda desses animais. Todos os animais foram eutanasiados no mesmo dia, o sangue coletado para an?lise de T3 ng/dL, T4 ?g/dL, e TSH ng/mL pela t?cnica de Radioimunoensaio. Aprova??o pelo comit? de ?tica da UFRRJ N?003/2015. Ap?s an?lise, observamos perda do peso corporal tanto nas f?meas quanto nos machos e uma diminui??o no peso relativo da hip?fise apenas nos machos do grupo T. Por outro lado, o peso relativo da adrenal se manteve reduzido no grupo T dos machos e aumentado no grupo T e TP24 das f?meas. Os n?veis s?ricos de TSH nos machos aumentaram com o exerc?cio nos grupos T, normalizando com a priva??o de 24 horas e retornando ao aumento no grupo TP24R. A PSP foi capaz de provocar um aumento nos n?veis de T3 nos grupos TP24 e TP96 dos machos, e nas f?meas n?o foi observado altera??es significativas. Quanto aos valores de T4 nos machos, n?o foi constatado altera??es significativas e nas f?meas a PSP foi capaz de elevar tais valores. Sugerimos que o exerc?cio de for?a esteja contribuindo para a prote??o dos impactos agressivos causados pela priva??o de sono paradoxal na fisiologia end?crina tanto em machos quanto em f?meas.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-10-24T20:09:04Z No. of bitstreams: 1 2014 - Nat?lia Santos da Cruz.pdf: 3689461 bytes, checksum: a8314ad212ff71c1ce3707089c4aca1b (MD5) Made available in DSpace on 2019-10-24T20:09:04Z (GMT). No. of bitstreams: 1 2014 - Nat?lia Santos da Cruz.pdf: 3689461 bytes, checksum: a8314ad212ff71c1ce3707089c4aca1b (MD5) Previous issue date: 2014-03-28 CAPES Sleep is considered essential for life and maintaining homeostasis. During pregnancy occur a number of changes, including sleep patterns with decreased amount of sleep in the third trimester. Is already proven that sleep deprivation triggers oxidative stress in different organs and the induction of oxidative stress during pregnancy can lead to congenital malformations, fetal death, hormonal changes, decreased body weight gain in mother and offspring, however, no studies in the literature evaluating the effects of sleep restriction on thyroid function during pregnancy. Therefore we aimed to study the effect of sleep restriction on thyroid function and metabolism of iodothyronines extrathyroid in pregnant rats submitted to sleep restriction, in addition to evaluating the same parameters in their offspring. Virgin females (200-250g), in proestrus, were placed with males. The presence of spermatozoa in vaginal smear defined gestational day 1 and females were divided into control (C, n=15) and sleep restricted (SR, n=22). To sleep restriction used the Modified Multiple Platform Methodology starting the restriction on the 14th gestational day and ending on the 20th, when half of the rats (C=7 and SR=11) were euthanized and the remaining calved normally (C=8 and SR=11), with remaining until weaning puppies when they were euthanized along with part of the male pups (C=4 and SR=8) and female (C=6 and SR=7), the female pups (C=4 and SR=13) remaining were euthanized at 60 days of life. Body weight was followed during pregnancy. Thyroid, adrenal and pituitary were weighed for analysis of the absolute and relative weight. T4 (?g/dL), T3 (ng/dL) and corticosterone (ng/ml) serum were analyzed by RIA. The deiodinase type 2 activity (D2, expressed in fmol T4/min.mg.ptn) was evaluated in the pituitary, brown adipose tissue (BAT), hypothalamus and hippocampus. The deiodinase type 1 activity (D1, expressed as pmol rT3/min.mg.ptn) was evaluated in the liver, kidney, thyroid and pituitary. In the statistical test T-Student (p

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-10-24T19:57:17Z No. of bitstreams: 1 2014 - Cristine de Paula Nascimento Castro.pdf: 1241417 bytes, checksum: 5e54b12492b26eb98805c461f9c8cbfc (MD5) Made available in DSpace on 2019-10-24T19:57:17Z (GMT). No. of bitstreams: 1 2014 - Cristine de Paula Nascimento Castro.pdf: 1241417 bytes, checksum: 5e54b12492b26eb98805c461f9c8cbfc (MD5) Previous issue date: 2014-03-27 CAPES The effects of sleep deprivation in endocrine system and the influence of ovarian hormones in this effect have been studied. However, sleep deprivation effects on thyroid function have not been reported in women. The purpose was to evaluate how paradoxical sleep deprivation (PSD) and sleep rebound affects the thyroid function and type 1 (D1) and 2 (D2) iodothyronine deiodinases activity of intact and ovariectomized (Ox) females rats. Intact and Ox female Wistar rats (200-250g) were subjected to PSD by modified multiple platform method. Intact females rats were distributed into 5 groups: Control; intacts subjected to 24 (PSD24) or 96 hours of PSD (PSD96); and respective sleep rebound of 24 hours (PSD24R and PSD96R) and ovariectomized in 6 groups: Sham-operated (FO), Ox, Ox subjected to 24 hours (OxPS24) or 96 hours of PSD (OxPS96) and respective sleep rebound of 24 hours (OxPS24R and OxPS96R). The animals were euthanized on the same day and blood and tissues were collected. Serum concentrarion of T3, T4, TSH, estradiol, progesterone and corticosterone as well as liver, kidney, thyroid and pituitary D1 activity and brown adipose tissue (BAT), pituitary and hippocampal D2 activity have been examined. Serum hormones were determined by radioimmunoassay . The measurements of thyroid radioiodide (125I-Na) uptake was done 15min and 2h after radioiodide administration for the analysis of the (sodium-iodide symporter) NIS and (thyroperoxidase) TPO in vivo activities, respectively. All the procedures were aproved by ethics committee of UFRRJ, N?. 23083.000948/2012-30. Intacts and Ox female rats subjected to 24 and 96 hours of PSD showed significant body weight loss and increased adrenal weight. PSD produced increased T3 and decreased TSH in intact female and there were rebound effects only TSH; while Ox rats showed reduced T4(PSP96) and progressive decline T3 level. Activity NIS and TPO were unchanged after PSD in intact female rats. There were no differences in serum corticosterone in spite of increased adrenal weight in Ox. D2 activity was increased in TAM and pituitary after PSD and only BAT activity was normalized by period rebound. D1 activity was increased in liver, kidney and thyroid after PSD in intacts female rats, in addition thyroid and kidney activity was normalized by rebound. D1 activity was decreased in liver and thyroid and increased in kidney and pituitary after PSD in Ox rats. Our findings indicated that PSD affects thyroid function in intact and castrated females. The increase in D1 and D2 activity after PSD might contribute to maintain serum T3 in intact rats. Furthermore, the decreased T4 and progressive decline T3 level showed in Ox confirm the importance of ovarian hormones to normal serum T4 in female rats which was subjected to PSD. Moreover, ovarian hormone might to be important to normalize the effect of sleep rebound in D1 activity but not in HT and D2 activity. O impacto da priva??o de sono sobre o sistema end?crino e a influ?ncia dos horm?nios sexuais femininos nas altera??es causadas pela priva??o de sono tem sido estudados, por?m o efeito da priva??o sobre a fun??o tireoidea ainda n?o foi avaliado em mulheres. Nosso objetivo foi estudar o efeito da priva??o de sono paradoxal (PSP) e do sono rebote de 24 horas sobre a fun??o tireoidea e atividade das desiodases tipo 1 (D1) e 2 (D2) de f?meas intactas e ovariectomizadas (Ox). Ratas Wistar intactas e ovariectomizadas (200-250g) foram submetidas ? PSP pela metodologia das plataformas m?ltiplas modificada. Ratas intactas foram distribu?das em Controle; PSP por 24 (PSP24) ou 96 horas (PSP96) e seus respectivos rebotes de 24h (PSP24R e PSP96R), enquanto ratas Ox foram distribu?das em Falsa-operada (FO), Ox, Ox PS por 24 (OxPS24) ou 96 horas (OxPS96) e seus respectivos rebotes de 24h (OxPS24R e OxPS96R). As f?meas foram submetidas ? eutan?sia no mesmo dia e o sangue foi coletado para an?lise de T3, T4, TSH, estradiol, progesterona e corticosterona por Radioimunoensaio. F?gado, rim, tireoide e hip?fise foram coletados para avalia??o da atividade D1 e tecido adiposo marrom (TAM), hip?fise e hipocampo para D2. A capta??o de iodeto pela tireoide ap?s 15min ou 2h da administra??o de iodeto marcado (NaI125) foi utilizada para a an?lise da atividade in vivo do co-transportador Na+I- (NIS) e tireoperoxidase (TPO), respectivamente. Todos os procedimentos foram aprovados pelo comit? de ?tica da UFRRJ N? 23083.000948/2012-30. Observamos perda de peso corporal e aumento da gl?ndula adrenal nas ratas intactas e castradas submetidas ? 24 e 96h de PSP. A PSP provocou aumento do T3 e diminui??o do TSH em ratas intactas, sendo apenas o TSH normalizado pelo rebote; enquanto as ratas Ox demonstraram diminui??o do T4 (OxPS96) e decl?nio progressivo do T3 ao longo do tempo de priva??o. A atividade do NIS e TPO n?o alteraram ap?s PSP nas intactas. A corticosterona n?o sofreu altera??o ap?s PSP, apesar do aumento de peso da gl?ndula adrenal nas ratas Ox. A PSP provocou aumento da atividade D2 no TAM, normalizada pelo rebote, e na hip?fise em ratas intactas e Ox. J? a atividade da D1 hep?tica, renal e tireoidea aumentou em ratas intactas PSP, havendo normaliza??o pelo rebote no rim e tireoide; enquanto ratas OxPS demonstraram redu??o da D1 hep?tica e tireoidea e aumento na D1 renal e hipofis?ria. Em conclus?o, a priva??o de sono de 24 e 96h provocaram altera??es na fun??o tireoidea de ratas intactas e castradas. Sugerimos que o aumento da atividade da D1 e D2 observado ap?s PSP esteja contribuindo para a manuten??o do T3 s?rico, enquanto a redu??o do T4 e decl?nio progressivo do T3 observado nas ratas castradas evidenciam a import?ncia dos horm?nios sexuais femininos na manuten??o dos n?veis de T4 em ratas privadas de sono. O efeito normalizador do sono rebote sobre a atividade D1 parece ser dependente de horm?nios gonadais femininos, mas independente no caso de horm?nios tireoideos e D2.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-11-05T20:51:01Z No. of bitstreams: 1 2014 - Rodrigo Rodrigues da Concei??o.pdf: 1589723 bytes, checksum: a51b86419fd32fad3eebb6a7a48ea970 (MD5) Made available in DSpace on 2019-11-05T20:51:01Z (GMT). No. of bitstreams: 1 2014 - Rodrigo Rodrigues da Concei??o.pdf: 1589723 bytes, checksum: a51b86419fd32fad3eebb6a7a48ea970 (MD5) Previous issue date: 2014-02-25 Strength training is known for being a method which increases the neuromuscular functional capacity (Deschenes & Kramer, 2002). This type of training has become a popular form of physical activity and various health benefits have been shown (STRENGHT NATIONAL ASSOCIATION POSITION STATEMENT AND CONDITION, 2001). Moreover thyroid hormones (triiodothyronine, thyroxine and T3, T4) are essential for normal growth and development. Despite the great importance of thyroid hormones, There are currently no studies that evaluate the effect of strength training on thyroid function, however the purpose of this study was to evaluate the acute and chronic modulation of the hypothalamic-pituitary-thyroid axis as well as the activity of iodothyronine desiodades type 1 (D1) and type 2 (D2) in rats that underwent the isometric strength training. To verify the acute effects, the animals were randomly divided into 5 groups: Baseline, 0, 30, 60 and 120 min following training. To the study of chronic responses, the animals were divided into 2 groups: Control and Training. In the study of the acute effects, there were significant increases in serum TSH levels and activity of the co transporter sodium-iodide symporter 0 min after exercise compared to baseline, however since T3 levels were only higher in control than in the trained, 120 min post training. D2 activity in TAM decreased significantly in group 120 min when compared with the basal group, the same occurring with pituitary D1. Which increase in group 120 min when compared with the basal group. With regard to chronic effects, we found significant increases in serum levels of T4, compared with the control group with trained by 8 weeks of isometric strength training. In serum T3, no significant differences were observed in control and training groups, the opposite occurred with respect to serum levels of corticosterone, which found a significant reduction and concentration when compared with the control with the trained by 8 weeks of isometric training strength. In TAM found significant reduction in D2 activity after 8 weeks of training. However we observe, in the soleus muscle, statistically significant decrease after 8 weeks of training. In liver and kidney we found significant increase in D1activity after 8 weeks of training. Our results demonstrate that strength training modulates the HPT axis and that this modulation is related to changes in the activity of iodothyronine deiodinases. O treinamento de for?a ? conhecido por ser um m?todo que aumenta a capacidade funcional neuromuscular (DESCHENES & KRAMER, 2002). Este tipo de treinamento tem se tornado uma forma popular de atividade f?sica e v?rios benef?cios para a sa?de j? foram evidenciados (NATIONAL STRENGHT AND CONDITION ASSOCIATION POSITION STATEMENT, 2001). Por outro lado os horm?nios tire?ideos (triiodotironina, T3 e tiroxina, T4) s?o essenciais para o crescimento e desenvolvimento normais. Apesar da grande import?ncia dos horm?nios tire?ideos, n?o h? estudos que avaliem o efeito do treinamento de for?a sobre a fun??o tireoidea, todavia o objetivo deste trabalho foi avaliar a modula??o aguda e cr?nica do eixo hipot?lamo-hip?fise-tireoide bem como a atividade das iodotironinas desiodades do tipo 1 (D1) e do Tipo 2 (D2) em ratos submetidos ao treinamento de for?a isom?trico. Para verificar os efeitos agudos, os animais foram divididos aleatoriamente em 5 grupos: Basal, 0, 30, 60 e 120 min ap?s o treinamento. Para o estudo das repostas cr?nicas, os animais foram distribu?dos em 2 grupos: Controle e Treinando. No estudo dos efeitos agudos, ocorreram aumentos significativas nos n?veis s?ricos do TSH e da atividade do co-transportador s?dio - iodeto 0 min ap?s o exerc?cio, quando comparados ao basal, j? os n?veis de T3 s? foram maiores no Treinado que no Controle 120 min p?s treinamento. N?o observamos diferen?as significativas no T4 e na corticosterona s?ricos entre os grupos. A atividade da D2 no TAM diminuiu significativa no grupo 120 min quando comparamos com o grupo Basal, o mesmo ocorrendo com a D1 na hip?fise. Que apresentou aumento significativo no grupo 120 min quando comparamos com o grupo Basal. Com rela??o aos efeitos cr?nicos, verificamos aumentos significativos nos n?veis s?ricos do T4, quando comparados com o grupo controle com os grupos treinado por 8 semanas. Nas concentra??es s?ricas de T3 n?o foram observadas diferen?as significativas nos diferentes grupos Controle e Treinado o oposto ocorreu com rela??o aos n?veis s?ricos de corticosterona, que verificamos redu??o significativa e sua concentra??o quando comparados com o grupo controle com os grupos treinado por 8 semanas. No TAM encontramos redu??o significativa na atividade da D2 ap?s 8 semanas de treinamento. Por?m observamos, no m?sculo Solear, redu??o significativa ap?s 8 semanas de treinamento. No f?gado e no rim encontramos aumento significativo da atividade da D1 ap?s 8 semanas de treinamento. Nossos resultados demonstram que o treinamento de for?a modula o eixo HPT e que tal modula??o esta relacionada com mudan?as na atividade da iodotironina desiodases.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-08-13T13:31:20Z No. of bitstreams: 1 2012 - Jorge Andr? Vergueiro Santana.pdf: 1034594 bytes, checksum: 278eccfd8bdd364eecb4631a64f6e1aa (MD5) Made available in DSpace on 2020-08-13T13:31:20Z (GMT). No. of bitstreams: 1 2012 - Jorge Andr? Vergueiro Santana.pdf: 1034594 bytes, checksum: 278eccfd8bdd364eecb4631a64f6e1aa (MD5) Previous issue date: 2012-08-27 The abuse of anabolic androgenic steroids (AAS), and off the sports scene, it is currently in big public health problem. Although studies on behavioral changes induced by AAS and electrolyte are well known, the same is not observed when these parameters are analyzed together (in the same study). In addition, late changes (in adulthood) induced by the use of AAS abuse in adolescence are even more rare. Thus, the main objective of this study was to assess possible behavioral changes, electrolyte and rat autonomic sedentary or physically active adults undergoing treatment with AAS in the juvenile phase. This study was submitted to the Ethics Committee of University Rural of Rio de Janeiro (CEPBE/IB/UFRRJ-014/2008), and followed the guidelines proposed by the "Guide for the Care and Use of Laboratory Animals" (National Institute of North American Health). A total of 40 young Wistar rats (40 days) and 10 pairs of residents (90 days). The animals were randomly divided into the following groups: proprionate treated with testosterone (PT) at a dose of 5mg/kg/day (im) or vehicle (corn oil) in the same volume and route of administration (control group, CTR) for 5 weeks uninterrupted. Both groups were subdivided into sedentary (or PTsed CTRsed) and physically active (or PTexe CTRexe). In the fourth week of administration (9 weeks of age), animals were tested for aggressiveness and the results showed a significant increase of this parameter (P

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-07-07T15:31:32Z No. of bitstreams: 1 2012 - Nayana Coutinho Rodrigues.pdf: 1163979 bytes, checksum: 66eea500c0364def1696d4791d0cb495 (MD5) Made available in DSpace on 2020-07-07T15:31:32Z (GMT). No. of bitstreams: 1 2012 - Nayana Coutinho Rodrigues.pdf: 1163979 bytes, checksum: 66eea500c0364def1696d4791d0cb495 (MD5) Previous issue date: 2012-05-02 FAPERJ - Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do Rio de Janeiro Modern life shortened sleep time and the consequences of the sleep loss have been examined in humans and animal models. Considering that the complete association between thyroid function and sleep loss has not been fully investigated, the aim of this study was to analyze thyroid function and type 2 deiodinase (D2) activity during paradoxical sleep deprivation (PSD) and sleep restriction (SR) and after 24 hours of rebound sleep period. Male Wistar rats (200-250g) underwent sleep deprivation by modified multiple platform method. The animals were assigned in 7 groups: control (n=11); PSD for 24 (n=15) and 96 hours (n=13); respective rebound groups (PSD24R, n=12 and PSD96R, n=14); SR for 21 days (n=14); and SR21 with rebound of 24 hours (SR21R, n=15). All animals were weighed and euthanized on the same day. Thyroid gland was weighed and blood samples were collected for T3 and T4 analysis by Electrochemiluminescence, corticosterone and TSH analysis by RIE. Pituitary gland and brown adipose tissue (BAT) were used for D2 activity in 6 groups (n=5/group). To evaluate the in vivo Na+/I symporter (NIS) function, the animals received Na-125I (250,000 dpm, i.p.) 15 min before decapitation, and the radioactivity of the thyroid glands was measured using a gamma counter (Wizard). The body weight gain (?) decreased in PSD24, PSD96 and PSD96R and rebound period was able to normalize these values only in 24 hour of sleep deprivation group, SR and its rebound period also decreased the body weight gain. The absolute and relative thyroid weight and NIS activity did not significantly change. Whilst serum T3 increased in all groups in relation to control. Serum T4 decreased in PSD24, PSD96 and PSD96R in relation to control. Serum TSH decreased in PSD24, PSD24R and PSD96 compared to control, whereas the rebound period was able to normalize TSH values only in 96 hour. In BAT, D2 activity increased only in PSD 24 or 96h groups and in pituitary D2 decreased in PSD24, PSD96R and SR21R. Serum T3 increased after selective and chronic sleep loss, and the sleep rebound was not able to normalize these changes. Conversely, T4 decreased only after paradoxical sleep deprivation, which can be explained by decreased serum TSH and increased D2 activity in BAT. Sleep rebound normalized T4 values after acute paradoxical sleep deprivation (24h), suggesting differential modulation of thyroid function in relation to chronic and selective sleep loss. A vida moderna vem encurtando o tempo de sono e as consequ?ncias da diminui??o do sono tem sido estudada em humanos e modelos animais. Considerando que a rela??o real entre a priva??o de sono e a fun??o tire?idea n?o foi totalmente elucidada, o objetivo deste trabalho foi avaliar a fun??o tire?idea e a atividade da desiodase tipo 2 (D2) durante a priva??o de sono paradoxal (PSP) e restri??o de sono (RS) e ap?s per?odo de sono rebote de 24 horas. Ratos machos (200-250g) foram submetidos ? priva??o de sono pela metodologia das plataformas m?ltiplas modificada. Os animais foram distribu?dos em 7 grupos: Controle (n=11); PSP por 24 (n=15) e 96 horas (n=13); com seus respectivos grupos rebote (PSP24R, n=12 e PSP96R, n=14); RS por 21 dias (n=14); e com o per?odo de rebote de 24 horas (RS21R, n=15). Todos os animais foram pesados e eutanasiados no mesmo dia. A gl?ndula tireoide foi pesada, sangue coletado para an?lise de T3 e T4 por Eletroquimioluminesc?ncia, corticosterona e TSH por Radioimunoensaio. A gl?ndula hip?fise e o tecido adiposo marrom (TAM) foram usados para analisar a atividade da D2 em 6 grupos (n=5/grupo). Para avaliar a atividade in vivo do co-transportador Na+/I (NIS), os animais receberam Na-125I (250.000 dpm, i.p.) e ap?s 15 minutos os animais foram eutanasiados e a radiatividade da gl?ndula foi mensurada utilizando um contador de part?culas gama (Wizard). O ganho de peso (?) diminuiu nos grupos PSP24, PSP96 e PSP96R e o per?odo de rebote foi capaz de normalizar esses valores apenas na em 24 horas de priva??o de sono, a RS e seu per?odo de rebote tamb?m foram capazes de diminuir o ganho de peso corporal. O peso absoluto e relativo da gl?ndula tireoide e a atividade do NIS n?o tiveram diferen?as significativas. Por outro lado, os n?veis s?ricos de T3 aumentaram em todos os grupos em rela??o ao grupo controle. Os n?veis s?ricos do T4 diminu?ram nos grupos PSP24, PSP96 e PSP96R em rela??o ao controle. Os n?veis s?ricos de TSH diminu?ram nos grupos PSP24, PSP24R e PSP96, entanto o per?odo de rebote foi capaz de normalizar os valores de TSH em 96 horas. No TAM a atividade da D2 aumentou apenas nos grupos PSP por 24 ou 96 horas, e na hip?fise a D2 diminuiu nos grupos PSP24, PSP96R e RS21R. Os n?veis s?ricos de T3 aumentaram na priva??o de sono seletiva e cr?nica, e o per?odo de rebote n?o foi capaz de normalizar estas altera??es. Controversamente, o T4 diminuiu apenas na priva??o de sono paradoxal, o que pode ser explicado pela diminui??o dos n?veis de TSH e aumento da atividade da D2 no TAM. O sono rebote normalizou os valores de T4 ap?s priva??o de sono paradoxal aguda (24 horas), sugerindo que a modula??o da fun??o tire?idea ? diferente em rela??o ? priva??o de sono seletiva e cr?nica.