Search

AKNA is identified as a gene that regulates inflammation, immune response, and Epithelial–Mesenchymal Transition (EMT), which plays an important role in the progression of epithelial ovarian cancer. In this study, we analyzed the genotype and allele distribution as well as 3D modeling of one of the AKNA rs10817595 (−1372 C>A). The distribution of genotypes and alleles was analyzed using the T-ARMS PCR method on 63 ovarian cancer samples and 65 controls. AKNA mRNA expression was analyzed using qRT-PCR on 35 low-grade and 28 high-grade samples. Fifteen low-grade and 12 high-grade samples were analyzed for AKNA protein levels using immunohistochemistry. A 3D model of protein structure was constructed using AlphaFold. Significant differences in AKNA protein levels were found. However, no significant correlation was found for relative AKNA mRNA expression with protein levels. This result is thought to be related to decreased immune system response, increased inflammation, and increased EMT in epithelial ovarian cancer. AKNA gene variant (−1372 C>A) can cause a decrease in mRNA and protein levels in the low-grade and high-grade groups, so it has the potential as a genetic susceptibility factor in epithelial ovarian cancer.

The result of science literacy in Indonesian students has been satisfactorily yet. The government has introduced some new learning strategies. The research is aimed to finding some learning strategies can improved literacy science. The research was conducted in Indonesian school involving 213 participants who are divided into study groups using several learning strategies. Research involved all student on 10 th grade in that school. Improvement of science literacy was analyzed by N-gain and comparing some strategies of learning was analyzed by Anova followed Turkey. The results shows the impovement of science literacy in the learning by using scientific approach was not as good as inquiry approach, Argument Driven Inquiry (ADI), and Science Technology Education Matematics (STEM). The results of science literacy finding the improvement in science literacy of students who learn by using scientific approach comparing using scientific approach is similar to the one using Science Technology Scociety (STS) strategy.