422 results on '"Miguel Blanca"'
Search Results
2. Allergenic Properties and Molecular Characteristics of PR-1 Proteins
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Andrea Wangorsch, Stephan Scheurer, Miguel Blanca, Natalia Blanca-Lopez, María Luisa Somoza, and Laura Martín-Pedraza
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pathogenesis-related protein-1 ,PR-1 ,allergen ,occupational allergy ,allergy diagnosis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Only a small fraction of proteins in plants and animals are classified as allergens. The allergenic properties are frequently attributed to certain functional characteristics of the proteins, such as a role in the plant defense against biotic and abiotic stress, to achieve the systematic acquired resistance. In line with this, eight members out of 17 functional pathogenesis-related (PR) protein families have been characterized as allergens. The present review summarizes the molecular features and allergenic significance of allergens of the PR-1 family. Not many allergens have been identified as belonging to this protein family, with most of them having a pollen origin, like mugwort or Bermuda grass. Molecular and structural features of allergenic PR-1 proteins are discussed and attributed to their IgE-reactive properties, clinical manifestation, and cross-reactivity among different foods and inhalants. more...
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- 2022
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3. Lack of Major Involvement of Common CYP2C Gene Polymorphisms in the Risk of Developing Cross-Hypersensitivity to NSAIDs
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Yolanda Macías, Jesús M. García-Menaya, Manuel Martí, Concepción Cordobés, Raquel Jurado-Escobar, José A. Cornejo-García, María J. Torres, Natalia Blanca-López, Gabriela Canto, Miguel Blanca, José J. Laguna, Joan Bartra, Ana Rosado, Javier Fernández, Elena García-Martín, and José A. G. Agúndez more...
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CYP2C8 ,CYP2C9 ,CYP2C19 ,NSAID ,polymorphisms ,hypersensitivity ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Cross-hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs) is a relatively common, non-allergic, adverse drug event triggered by two or more chemically unrelated NSAIDs. Current evidence point to COX-1 inhibition as one of the main factors in its etiopathogenesis. Evidence also suggests that the risk is dose-dependent. Therefore it could be speculated that individuals with impaired NSAID biodisposition might be at increased risk of developing cross-hypersensitivity to NSAIDs. We analyzed common functional gene variants for CYP2C8, CYP2C9, and CYP2C19 in a large cohort composed of 499 patients with cross-hypersensitivity to NSAIDs and 624 healthy individuals who tolerated NSAIDs. Patients were analyzed as a whole group and subdivided in three groups according to the main enzymes involved in the metabolism of the culprit drugs as follows: CYP2C9, aceclofenac, indomethacin, naproxen, piroxicam, meloxicam, lornoxicam, and celecoxib; CYP2C8 plus CYP2C9, ibuprofen and diclofenac; CYP2C19 plus CYP2C9, metamizole. Genotype calls ranged from 94 to 99%. No statistically significant differences between patients and controls were identified in this study, either for allele frequencies, diplotypes, or inferred phenotypes. After patient stratification according to the enzymes involved in the metabolism of the culprit drugs, or according to the clinical presentation of the hypersensitivity reaction, we identified weak significant associations of a lower frequency (as compared to that of control subjects) of CYP2C8*3/*3 genotypes in patients receiving NSAIDs that are predominantly CYP2C9 substrates, and in patients with NSAIDs-exacerbated cutaneous disease. However, these associations lost significance after False Discovery Rate correction for multiple comparisons. Taking together these findings and the statistical power of this cohort, we conclude that there is no evidence of a major implication of the major functional CYP2C polymorphisms analyzed in this study and the risk of developing cross-hypersensitivity to NSAIDs. This argues against the hypothesis of a dose-dependent COX-1 inhibition as the main underlying mechanism for this adverse drug event and suggests that pre-emptive genotyping aiming at drug selection should have a low practical utility for cross-hypersensitivity to NSAIDs. more...
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- 2021
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4. Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
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Yulia Dorofeeva, Paolo Colombo, Miguel Blanca, Adriano Mari, Roman Khanferyan, Rudolf Valenta, and Margarete Focke-Tejkl
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Medicine ,Science - Abstract
Abstract The weed wall pellitory, Parietaria judaica, is one the most important pollen allergen sources in the Mediterranean area causing severe symptoms of hay fever and asthma in allergic patients. We report the expression of the major Parietaria allergens, Par j 1 and Par j 2 which belong to the family of lipid transfer proteins, in insect cells. According to circular dichroism analysis and gel filtration, the purified allergens represented folded and monomeric proteins. Insect cell-expressed, folded Par j 2 exhibited higher IgE binding capacity and more than 100-fold higher allergenic activity than unfolded Escherichia coli-expressed Par j 2 as demonstrated by IgE ELISA and basophil activation testing. IgE ELISA inhibition assays showed that Par j 1 and Par j 2, contain genuine and cross-reactive IgE epitopes. IgG antibodies induced by immunization with Par j 2 inhibited binding of allergic patients IgE to Par j 1 only partially. IgE inhibition experiments demonstrated that insect cell-expressed Par j 1 and Par j 2 together resembled the majority of allergenic epitopes of the Parietaria allergome and therefore both should be used for molecular diagnosis and the design of vaccines for allergen-specific immunotherapy of Parietaria allergy. more...
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- 2019
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5. Subjects develop tolerance to Pru p 3 but respiratory allergy to Pru p 9: A large study group from a peach exposed population.
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Maria Luisa Somoza, Natalia Pérez-Sánchez, Laura Victorio-Puche, Laura Martín-Pedraza, Angel Esteban Rodríguez, Natalia Blanca-López, Eva Abel Fernández González, María Ruano-Zaragoza, Ana Prieto-Moreno Pfeifer, Enrique Fernández Caldas, Miriam Morán Morales, Francisco Javier Fernández Sánchez, José Damián López Sánchez, Teodorikez Wilfox Jiménez Rodríguez, José Luis Subiza Garrido-Lestache, Gabriela Canto Díez, Miguel Blanca Gómez, and José Antonio Cornejo-García more...
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Medicine ,Science - Abstract
Peach tree allergens are present in fruit, pollen, branches, and leaves, and can induce systemic, respiratory, cutaneous, and gastrointestinal symptoms. We studied the capacity of peach fruit/Pru p 1, Pru p 3, Pru p 4, Pru p 7 and peach pollen/Pru p 9 for inducing symptoms following oral or respiratory exposure in a large group of subjects. We included 716 adults (aged 21 to 83 y.o.) exposed to peach tree pollen and fruit intake in the study population. Participants completed a questionnaire and were skin tested with a panel of inhalant and food allergens, including peach tree pollen, Pru p 9 and peach fruit skin extract. Immunoglobulin E antibodies (SIgE) to Pru p 1, Pru p 3, Pru p 4 and Pru p 7 were quantified. Sensitised subjects underwent oral food challenge with peach fruit and nasal provocation test with peach tree pollen and Pru p 9. The prevalence of sensitisation to peach fruit was 5% and most of these had SIgE to Pru p 3, with a very low proportion to Pru p 4 SIgE and no SIgE to Pru p 1 and Pru p 7. In only 1.8%, anaphylaxis was the clinical entity induced. Cases with positive skin tests to peach and SIgE to Pru p 3 presented a good tolerance after oral challenge with peach fruit. The prevalence of skin sensitisation to peach tree pollen was 22%, with almost half recognising Pru p 9. This induced respiratory symptoms in those evaluated by nasal provocation. In a large population group exposed to peach fruit and peach tree pollen, most individuals were tolerant, even in those with SIgE to Pru p 3. A positive response to Pru p 9 was associated with respiratory allergy. more...
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- 2021
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6. Amoxicillin Inactivation by Thiol-Catalyzed Cyclization Reduces Protein Haptenation and Antibacterial Potency
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María A. Pajares, Tahl Zimmerman, Francisco J. Sánchez-Gómez, Adriana Ariza, María J. Torres, Miguel Blanca, F. Javier Cañada, María I. Montañez, and Dolores Pérez-Sala
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amoxicillin ,β-lactam antibiotics ,inactivation mechanism ,redox regulation ,protein adducts ,thiol groups ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Serum and cellular proteins are targets for the formation of adducts with the β-lactam antibiotic amoxicillin. This process could be important for the development of adverse, and in particular, allergic reactions to this antibiotic. In studies exploring protein haptenation by amoxicillin, we observed that reducing agents influenced the extent of amoxicillin-protein adducts formation. Consequently, we show that several thiol-containing compounds, including dithiothreitol, N-acetyl-L-cysteine, and glutathione, perform a nucleophilic attack on the amoxicillin molecule that is followed by an internal rearrangement leading to amoxicillin diketopiperazine, a known amoxicillin metabolite with residual activity. Increased diketopiperazine conversion is also observed with human serum albumin but not with L-cysteine, which mainly forms the amoxicilloyl amide. The effect of thiols is catalytic and can render complete amoxicillin conversion. Interestingly, this process is dependent on the presence of an amino group in the antibiotic lateral chain, as in amoxicillin and ampicillin. Furthermore, it does not occur for other β-lactam antibiotics, including cefaclor or benzylpenicillin. Biological consequences of thiol-mediated amoxicillin transformation are exemplified by a reduced bacteriostatic action and a lower capacity of thiol-treated amoxicillin to form protein adducts. Finally, modulation of the intracellular redox status through inhibition of glutathione synthesis influenced the extent of amoxicillin adduct formation with cellular proteins. These results open novel perspectives for the understanding of amoxicillin metabolism and actions, including the formation of adducts involved in allergic reactions. more...
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- 2020
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7. Correction: Pru p 9, a new allergen eliciting respiratory symptoms in subjects sensitized to peach tree pollen.
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Miguel Blanca, Laura Victorio Puche, María Garrido-Arandia, Laura Martin-Pedraza, Alejandro Romero Sahagún, José Damian López-Sánchez, Carmen Galán, Antonio Marin, Mayte Villalba, Araceli Díaz-Perales, and Maria Luisa Somoza more...
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Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0230010.].
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- 2020
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8. Pru p 9, a new allergen eliciting respiratory symptoms in subjects sensitized to peach tree pollen.
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Miguel Blanca, Laura Victorio Puche, María Garrido-Arandia, Laura Martin-Pedraza, Alejandro Romero Sahagún, José Damian López-Sánchez, Carmen Galán, Antonio Marin, Mayte Villalba, Araceli Díaz-Perales, and Maria Luisa Somoza more...
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Medicine ,Science - Abstract
Peach tree (PT) pollen sensitization is highly prevalent in subjects living in areas where this tree is widely cultivated. None of the allergens responsible for these sensitizations have been identified so far. Our aim was to identify the most relevant PT pollen allergens and analyze their capacity for inducing respiratory symptoms. We studied sixty-two individuals sensitized to PT pollen who developed symptoms after its exposure. The IgE binding profile on peach pollen extract by means of immunoblotting using sera from these subjects was analyzed. Protein extract was fractionated by anion-exchange chromatography and HPLC, fractions run in SDS-PAGE and proteins were identified from IgE-binding bands by mass spectrometry. Several allergenic proteins in the PT pollen extract were recognized by patients' IgE: a glucan endo-1,3-beta-glucosidase-like, a polygalacturonase, an UTP-glucose-1-phosphate uridylyltransferase and a PR-1a protein. This PR-1a protein is a novel allergen frequently recognized with a molecular mass of 18 kDa, named as Pru p 9 following the WHO-IUIS nomenclature. Skin Prick Test (SPT) performed with this allergen was positive in 41% of the PT pollen-sensitized clinical cases. Most of them had rhinitis or rhinoconjunctivitis, but a significant percentage experienced asthma with seasonal symptoms during the period of PT flowering. Nasal Provocation test (NPT) with Pru p 9 was positive in all cases with positive SPT to this new allergen eliciting nasal symptoms similar to those challenged with PT pollen. We demonstrate that PT pollen can induce sensitization and allergy in an exposed population, being Pru p 9 a relevant allergen responsible of respiratory symptoms. Considering the extensive peach worldwide production with a large number of people involved, our results add a great value for the diagnosis and management of subjects allergic to this pollen. more...
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- 2020
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9. Identification of Novel Biomarkers for Drug Hypersensitivity After Sequencing of the Promoter Area in 16 Genes of the Vitamin D Pathway and the High-Affinity IgE Receptor
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Gemma Amo, Manuel Martí, Jesús M. García-Menaya, Concepción Cordobés, José A. Cornejo-García, Natalia Blanca-López, Gabriela Canto, Inmaculada Doña, Miguel Blanca, María José Torres, José A. G. Agúndez, and Elena García-Martín more...
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Next-Generation Sequencing (NGS) ,vitamin D ,high-affinity IgE receptor (FCεRI) ,NSAIDs (non-steroidal anti-inflammatory drugs) ,beta-lactam antibiotic ,drugs hypersensitivity reactions ,Genetics ,QH426-470 - Abstract
The prevalence of allergic diseases and drug hypersensitivity reactions (DHRs) during recent years is increasing. Both, allergic diseases and DHRs seem to be related to an interplay between environmental factors and genetic susceptibility. In recent years, a large effort in the elucidation of the genetic mechanisms involved in these disorders has been made, mostly based on case-control studies, and typically focusing on isolated SNPs. These studies provide a limited amount of information, which now can be greatly expanded by the complete coverage that Next Generation Sequencing techniques offer. In this study, we analyzed the promoters of sixteen genes related to the Vitamin D pathway and the high-affinity IgE receptor, including FCER1A, MS4A2, FCER1G, VDR, GC, CYP2R1, CYP27A1, CYP27B1, CYP24A1, RXRA, RXRB, RXRG, IL4, IL4R, IL13, and IL13RA1. The study group was composed of patients with allergic rhinitis plus asthma (AR+A), patients with hypersensitivity to beta-lactams (BLs), to NSAIDs including selective hypersensitivity (SH) and cross-reactivity (CR), and healthy controls without antecedents of atopy or adverse drug reactions. We identified 148 gene variations, 43 of which were novel. Multinomial analyses revealed that three SNPs corresponding to the genes FCER1G (rs36233990 and rs2070901), and GC (rs3733359), displayed significant associations and, therefore, were selected for a combined dataset study in a cohort of 2,476 individuals. The strongest association was found with the promoter FCER1G rs36233990 SNP that alters a transcription factor binding site. This SNP was over-represented among AR+A patients and among patients with IgE-mediated diseases, as compared with control individuals or with the rest of patients in this study. Classification models based on the above-mentioned SNPs were able to predict correct clinical group allocations in patients with DHRs, and patients with IgE-mediated DHRs. Our findings reveal gene promoter SNPs that are significant predictors of drug hypersensitivity, thus reinforcing the hypothesis of a genetic predisposition for these diseases. more...
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- 2019
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10. Next-Generation Sequencing of PTGS Genes Reveals an Increased Frequency of Non-synonymous Variants Among Patients With NSAID-Induced Liver Injury
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María Isabel Lucena, Elena García-Martín, Ann K. Daly, Miguel Blanca, Raúl J. Andrade, and José A. G. Agúndez
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PTGS1 ,PTGS2 ,next generation sequencing ,drug-induced liver injury ,COX1 ,COX2 ,Genetics ,QH426-470 - Abstract
Purpose: The etiopathogenesis of drug-induced liver injury (DILI) is still far from being elucidated. This study aims to the study of genetic variations in DILI, related to the drug target, and specifically in the genes coding for the cyclooxygenase enzymes.Methods: By using Next-generation Sequencing we analyzed the genes coding for COX enzymes (PTGS1 and PTGS2) in 113 individuals, 13 of which were patients with DILI caused by COX-inhibitors.Results: The key findings of the study are the increased frequency, among DILI patients, of SNPs causing alterations in transcription factor binding sites and non-synonymous PTGS gene variants, as compared to control subjects. Moreover, the association with non-synonymous SNPs was exclusive of DILI patients with late-onset (50 days or more) Pc < 0.001 as compared to DILI patients with early onset, or with control subjects.Conclusions: Our findings suggest an interaction of long-term exposure to COX inhibitors combined with functional variants of the COX enzymes in the risk of developing DILI. This is a novel observation that might have been overlooked by previous genetic studies on DILI because of the limited coverage of PTGS genes in exome chips. more...
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- 2019
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11. Risk and safety requirements for diagnostic and therapeutic procedures in allergology: World Allergy Organization Statement
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Marek L. Kowalski, Ignacio Ansotegui, Werner Aberer, Mona Al-Ahmad, Mubeccel Akdis, Barbara K. Ballmer-Weber, Kirsten Beyer, Miguel Blanca, Simon Brown, Chaweewan Bunnag, Arnaldo Capriles Hulett, Mariana Castells, Hiok Hee Chng, Frederic De Blay, Motohiro Ebisawa, Stanley Fineman, David B. K. Golden, Tari Haahtela, Michael Kaliner, Connie Katelaris, Bee Wah Lee, Joanna Makowska, Ulrich Muller, Joaquim Mullol, John Oppenheimer, Hae-Sim Park, James Parkerson, Giovanni Passalacqua, Ruby Pawankar, Harald Renz, Franziska Rueff, Mario Sanchez-Borges, Joaquin Sastre, Glenis Scadding, Scott Sicherer, Pongsakorn Tantilipikorn, James Tracy, Vera van Kempen, Barbara Bohle, G Walter Canonica, Luis Caraballo, Maximiliano Gomez, Komei Ito, Erika Jensen-Jarolim, Mark Larche, Giovanni Melioli, Lars K. Poulsen, Rudolf Valenta, and Torsten Zuberbier more...
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract One of the major concerns in the practice of allergy is related to the safety of procedures for the diagnosis and treatment of allergic disease. Management (diagnosis and treatment) of hypersensitivity disorders involves often intentional exposure to potentially allergenic substances (during skin testing), deliberate induction in the office of allergic symptoms to offending compounds (provocation tests) or intentional application of potentially dangerous substances (allergy vaccine) to sensitized patients. These situations may be associated with a significant risk of unwanted, excessive or even dangerous reactions, which in many instances cannot be completely avoided. However, adverse reactions can be minimized or even avoided if a physician is fully aware of potential risk and is prepared to appropriately handle the situation. Information on the risk of diagnostic and therapeutic procedures in allergic diseases has been accumulated in the medical literature for decades; however, except for allergen specific immunotherapy, it has never been presented in a systematic fashion. Up to now no single document addressed the risk of the most commonly used medical procedures in the allergy office nor attempted to present general requirements necessary to assure the safety of these procedures. Following review of available literature a group of allergy experts within the World Allergy Organization (WAO), representing various continents and areas of allergy expertise, presents this report on risk associated with diagnostic and therapeutic procedures in allergology and proposes a consensus on safety requirements for performing procedures in allergy offices. Optimal safety measures including appropriate location, type and required time of supervision, availability of safety equipment, access to specialized emergency services, etc. for various procedures have been recommended. This document should be useful for allergists with already established practices and experience as well as to other specialists taking care of patients with allergies. more...
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- 2016
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12. FCERI AND HISTAMINE METABOLISM GENE VARIABILITY IN SELECTIVE RESPONDERS TO NSAIDS
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Gemma Amo, José Antonio Cornejo-García, Jesus Miguel García-Menaya, Concepcion Cordobes, M Jose Torres, Gara Esguevillas, Cristobalina Mayorga, Carmen Martinez, Natalia Blanca Lopez, Gabriela Canto, Alfonso Ramos, Miguel Blanca, José A G Agúndez, and Elena García-Martín more...
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Histamine ,biomarkers ,Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) ,Hypersensitivity drug reactions ,Fcε RI ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The high-affinity IgE receptor (Fcε RI) is a heterotetramer of three subunits: Fcε RIα, Fcε RIβ and Fcε RIγ (αβγ2) encoded by three genes designated as FCER1A, FCER1B (MS4A2) and FCER1G, respectively. Recent evidence points to FCERI gene variability as a relevant factor in the risk of developing allergic diseases. Because Fcε RI plays a key role in the events downstream of the triggering factors in immunological response, we hypothesized that FCERI gene variants might be related with the risk of, or with the clinical response to, selective (IgE mediated) non-steroidal anti-inflammatory (NSAID) hypersensitivity.From a cohort of 314 patients suffering from selective hypersensitivity to metamizole, ibuprofen, diclofenac, paracetamol, acetylsalicylic acid (ASA), propifenazone, naproxen, ketoprofen, dexketoprofen, etofenamate, aceclofenac, etoricoxib, dexibuprofen, indomethacin, oxyphenylbutazone or piroxicam, and 585 unrelated healthy controls that tolerated these NSAIDs, we analyzed the putative effects of the FCERI SNPs FCER1A rs2494262, rs2427837 and rs2251746; FCER1B rs1441586, rs569108 and rs512555; FCER1G rs11587213, rs2070901 and rs11421. Furthermore, in order to identify additional genetic markers which might be associated with the risk of developing selective NSAID hypersensitivity, or which may modify the putative association of FCERI gene variations with risk, we analyzed polymorphisms known to affect histamine synthesis or metabolism, such as rs17740607, rs2073440, rs1801105, rs2052129, rs10156191, rs1049742 and rs1049793 in the HDC, HNMT and DAO genes.No major genetic associations with risk or with clinical presentation, and no gene-gene interactions, or gene-phenotype interactions (including age, gender, IgE concentration, antecedents of atopy, culprit drug or clinical presentation) were identified in patients. However, logistic regression analyses indicated that the presence of antecedents of atopy and the DAO SNP rs2052129 (GG) were strongly related (P more...
- Published
- 2016
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13. ASTHMA AND RHINITIS INDUCED BY SELECTIVE IMMEDIATE REACTIONS TO PARACETAMOL AND NON-STEROIDAL ANTI-INFLAMMATORY DRUGS IN ASPIRIN TOLERANT SUBJECTS
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Diana Pérez-Alzate, Natalia Blanca-López, Inmaculada Doña, José A G Agúndez, Elena García-Martín, José Antonio Cornejo-García, James Richard Perkins, Miguel Blanca, and Gabriela Canto
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Asthma ,Hypersensitivity ,Non-steroidal anti-inflammatory drugs ,paracetamol ,Immediate allergy ,rhinitis. ,Therapeutics. Pharmacology ,RM1-950 - Abstract
In subjects with non-steroidal anti-inflammatory drugs (NSAIDs)- exacerbated respiratory disease (NERD) symptoms are triggered by acetyl salicylic acid (ASA) and other strong COX-1 inhibitors, and in some cases by weak COX-1 or by selective COX-2 inhibitors. The mechanism involved is related to prostaglandin pathway inhibition and leukotriene release. Subjects who react to a single NSAID and tolerate others are considered selective responders, and often present urticaria and/or angioedema and anaphylaxis (SNIUAA). An immunological mechanism is implicated in these reactions. However, anecdotal evidence suggests that selective responders who present respiratory airway symptoms may also exist.Our objective was to determine if subjects might develop selective responses to NSAIDs/paracetamol that manifest as upper/lower airways respiratory symptoms. For this purpose we studied patients reporting asthma and/or rhinitis induced by paracetamol or a single NSAID that tolerated ASA. An allergological evaluation plus controlled challenge with ASA was carried out. If ASA tolerance was found, we proceeded with an oral challenge with the culprit drug. The appearance of symptoms was monitored by a clinical questionnaire and by measuring FEV1 and/or nasal airways volume changes pre and post challenge. From a total of 21 initial cases, we confirmed the appearance of nasal and/or bronchial manifestations in ten, characterised by a significant decrease in FEV1% and/or a decrease in nasal volume cavity after drug administration. All cases tolerated ASA.This shows that ASA tolerant subjects with asthma and/or rhinitis induced by paracetamol or a single NSAID without skin/systemic manifestations exist. Whether these patients represent a new clinical phenotype to be included within the current classification of hypersensitivity reactions to NSAIDs requires further investigation. more...
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- 2016
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14. High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.
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Francisca Gomez, Ana Aranda, Paloma Campo, Araceli Diaz-Perales, Natalia Blanca-Lopez, James Perkins, Maria Garrido, Miguel Blanca, Cristobalina Mayorga, and Maria José Torres
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Medicine ,Science - Abstract
BackgroundApple allergy manifests as two main groups of clinical entities reflecting different patterns of allergen sensitization: oral allergy syndrome (OAS) and generalized symptoms (GS).ObjectiveWe analysed the sensitization profile to a wide panel of different components of food allergens (rMal d 1, Mal d 2, rMal d 3, rMal d 4, rPru p 3, rBet v 1 and Pho d 2) for a population of Mediterranean patients with OAS and GS to apple.MethodsPatients (N = 81) with a history of apple allergy that could be confirmed by positive prick-prick test and/or double-blind-placebo-controlled food challenge (DBPCFC), were included. Skin prick test (SPT) and ELISA were performed using a panel of inhalant, fruit and nut allergens. ELISA and ELISA inhibition studies were performed in order to analyse the sensitization patterns.ResultsThirty-five cases (43.2%) had OAS and 46 (56.8%) GS. SPT showed a significantly higher number of positive results with peach, cherry and hazelnut in those with GS. ELISA showed a significantly high percentage of positive cases to rMal d 3, rMal d 4, rPru p 3 and Pho d 2 in patients with OAS and GS compared to controls, and to rBet v 1 in patients with OAS vs controls and between OAS and GS patients. Three different patterns of recognition were detected: positive to LTP (rMal d 3 or rPru p 3), positive to profilin (rMal d 4 and Pho d 2), or positive to both. There were also patients with rMal d 1 recognition who showed cross-reactivity to rBet v 1.ConclusionIn an apple allergy population with a high incidence of pollinosis different patterns of sensitization may occur. LTP is most often involved in those with GS. Profilin, though more prevalent in patients with OAS, has been shown to sensitise patients with both types of symptoms. more...
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- 2014
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15. Variants of CEP68 gene are associated with acute urticaria/angioedema induced by multiple non-steroidal anti-inflammatory drugs.
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José Antonio Cornejo-García, Carlos Flores, María C Plaza-Serón, Marialbert Acosta-Herrera, Natalia Blanca-López, Inmaculada Doña, María J Torres, Cristobalina Mayorga, Rosa M Guéant-Rodríguez, Pedro Ayuso, Javier Fernández, José J Laguna, José A G Agúndez, Elena García-Martín, Jean-Louis Guéant, Gabriela Canto, and Miguel Blanca more...
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Medicine ,Science - Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs) are the most consumed drugs worldwide because of their efficacy and utility in the treatment of pain and inflammatory diseases. However, they are also responsible for an important number of adverse effects including hypersensitivity reactions. The most important group of these reactions is triggered by non-immunological, pharmacological mechanisms catalogued under the denomination of cross-intolerance (CRI), with acute urticaria/angioedema induced by multiple NSAIDs (MNSAID-UA) the most frequently associated clinical entity. A recent genome-wide association study identified the gene encoding the centrosomal protein of 68 KDa (CEP68) as the major locus associated with aspirin intolerance susceptibility in asthmatics. In this study, we aimed to assess the role of this locus in susceptibility to CRI to NSAIDs by examining 53 common gene variants in a total of 635 patients that were classified as MNSAID-UA (n = 399), airway exacerbations (n = 110) or blended pattern (n = 126), and 425 controls. We found in the MNSAID-UA group a number of variants (17) associated (lowest p-value = 1.13 × 10(-6)), including the non-synonymous Gly74Ser variant (rs7572857) previously associated with aspirin intolerance susceptibility in asthmatics. Although not being significant in the context of multiple testing, eight of these variants were also associated with exacerbated respiratory disease or blended reactions. Our results suggest that CEP68 gene variants may play an important role in MNSAID-UA susceptibility and, despite the different regulatory mechanisms involved depending on the specific affected organ, in the development of hypersensitivity reactions to NSAIDs. more...
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- 2014
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16. Immunotherapy reduces allergen-mediated CD66b expression and myeloperoxidase levels on human neutrophils from allergic patients.
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Rocio Aroca, Cristina Chamorro, Antonio Vega, Inmaculada Ventura, Elisa Gómez, Ramón Pérez-Cano, Miguel Blanca, and Javier Monteseirín
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Medicine ,Science - Abstract
CD66b is a member of the carcinoembryonic antigen family, which mediates the adhesion between neutrophils and to endothelial cells. Allergen-specific immunotherapy is widely used to treat allergic diseases, and the molecular mechanisms underlying this therapy are poorly understood. The present work was undertaken to analyze A) the in vitro effect of allergens and immunotherapy on cell-surface CD66b expression of neutrophils from patients with allergic asthma and rhinitis and B) the in vivo effect of immunotherapy on cell-surface CD66b expression of neutrophils from nasal lavage fluid during the spring season. Myeloperoxidase expression and activity was also analyzed in nasal lavage fluid as a general marker of neutrophil activation.CD66b cell-surface expression is upregulated in vitro in response to allergens, and significantly reduced by immunotherapy (p more...
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- 2014
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17. Study of protein haptenation by amoxicillin through the use of a biotinylated antibiotic.
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Adriana Ariza, Daniel Collado, Yolanda Vida, María I Montañez, Ezequiel Pérez-Inestrosa, Miguel Blanca, María José Torres, F Javier Cañada, and Dolores Pérez-Sala
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Medicine ,Science - Abstract
Allergic reactions towards β-lactam antibiotics pose an important clinical problem. The ability of small molecules, such as a β-lactams, to bind covalently to proteins, in a process known as haptenation, is considered necessary for induction of a specific immunological response. Identification of the proteins modified by β-lactams and elucidation of the relevance of this process in allergic reactions requires sensitive tools. Here we describe the preparation and characterization of a biotinylated amoxicillin analog (AX-B) as a tool for the study of protein haptenation by amoxicillin (AX). AX-B, obtained by the inclusion of a biotin moiety at the lateral chain of AX, showed a chemical reactivity identical to AX. Covalent modification of proteins by AX-B was reduced by excess AX and vice versa, suggesting competition for binding to the same targets. From an immunological point of view, AX and AX-B behaved similarly in RAST inhibition studies with sera of patients with non-selective allergy towards β-lactams, whereas, as expected, competition by AX-B was poorer with sera of AX-selective patients, which recognize AX lateral chain. Use of AX-B followed by biotin detection allowed the observation of human serum albumin (HSA) modification by concentrations 100-fold lower that when using AX followed by immunological detection. Incubation of human serum with AX-B led to the haptenation of all of the previously identified major AX targets. In addition, some new targets could be detected. Interestingly, AX-B allowed the detection of intracellular protein adducts, which showed a cell type-specific pattern. This opens the possibility of following the formation and fate of AX-B adducts in cells. Thus, AX-B may constitute a valuable tool for the identification of AX targets with high sensitivity as well as for the elucidation of the mechanisms involved in allergy towards β-lactams. more...
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- 2014
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18. Hypersensitivity reactions to non beta-lactam antimicrobial agents, a statement of the WAO special committee on drug allergy
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Mario Sánchez-Borges, Bernard Thong, Miguel Blanca, Luis Felipe Chiaverini Ensina, Sandra González-Díaz, Paul A Greenberger, Edgardo Jares, Young-Koo Jee, Luciana Kase-Tanno, David Khan, Jung-Won Park, Werner Pichler, Antonino Romano, and Maria José Torres Jaén more...
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Antibiotics are used extensively in the treatment of various infections. Consequently, they can be considered among the most important agents involved in adverse reactions to drugs, including both allergic and non-allergic drug hypersensitivity [J Allergy Clin Immunol 113:832–836, 2004]. Most studies published to date deal mainly with reactions to the beta-lactam group, and information on hypersensitivity to each of the other antimicrobial agents is scarce. The present document has been produced by the Special Committee on Drug Allergy of the World Allergy Organization to present the most relevant information on the incidence, clinical manifestations, diagnosis, possible mechanisms, and management of hypersensitivity reactions to non beta-lactam antimicrobials for use by practitioners worldwide. more...
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- 2013
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19. Synergistic effect between amoxicillin and TLR ligands on dendritic cells from amoxicillin-delayed allergic patients.
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Maria J Sanchez-Quintero, Maria J Torres, Ana B Blazquez, Enrique Gómez, Tahia D Fernandez, Inmaculada Doña, Adriana Ariza, Inmaculada Andreu, Lidia Melendez, Miguel Blanca, and Cristobalina Mayorga
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Medicine ,Science - Abstract
Amoxicillin, a low-molecular-weight compound, is able to interact with dendritic cells inducing semi-maturation in vitro. Specific antigens and TLR ligands can synergistically interact with dendritic cells (DC), leading to complete maturation and more efficient T-cell stimulation. The aim of the study was to evaluate the synergistic effect of amoxicillin and the TLR2, 4 and 7/8 agonists (PAM, LPS and R848, respectively) in TLR expression, DC maturation and specific T-cell response in patients with delayed-type hypersensitivity (DTH) reactions to amoxicillin. Monocyte-derived DC from 15 patients with DTH to amoxicillin and 15 controls were cultured with amoxicillin in the presence or absence of TLR2, 4 and 7/8 agonists (PAM, LPS and R848, respectively). We studied TLR1-9 gene expression by RT-qPCR, and DC maturation, lymphocyte proliferation and cytokine production by flow cytometry. DC from both patients and controls expressed all TLRs except TLR9. The amoxicillin plus TLR2/4 or TLR7/8 ligands showed significant differences, mainly in patients: AX+PAM+LPS induced a decrease in TLR2 and AX+R848 in TLR2, 4, 7 and 8 mRNA levels. AX+PAM+LPS significantly increased the percentage of maturation in patients (75%) vs. controls (40%) (p=0.036) and T-cell proliferation (80.7% vs. 27.3% of cases; p=0.001). Moreover, the combinations AX+PAM+LPS and AX+R848 produced a significant increase in IL-12p70 during both DC maturation and T-cell proliferation. These results indicate that in amoxicillin-induced maculopapular exanthema, the presence of different TLR agonists could be critical for the induction of the innate and adaptive immune responses and this should be taken into account when evaluating allergic reactions to these drugs. more...
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- 2013
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20. Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon).
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Ana Aranda, Paloma Campo, Arantxa Palacin, Inmaculada Doña, Cristina Gomez-Casado, Luisa Galindo, Araceli Díaz-Perales, and Miguel Blanca
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Medicine ,Science - Abstract
BACKGROUND: Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. OBJECTIVE: To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma MATERIALS AND METHODS: An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. RESULTS: Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p more...
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- 2013
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21. The diamine oxidase gene is associated with hypersensitivity response to non-steroidal anti-inflammatory drugs.
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José A G Agúndez, Pedro Ayuso, José A Cornejo-García, Miguel Blanca, María J Torres, Inmaculada Doña, María Salas, Natalia Blanca-López, Gabriela Canto, Carmen Rondon, Paloma Campo, José J Laguna, Javier Fernández, Carmen Martínez, and Elena García-Martín more...
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Medicine ,Science - Abstract
UnlabelledNon-steroidal anti-inflammatory drugs (NSAIDs) are the drugs most frequently involved in hypersensitivity drug reactions. Histamine is released in the allergic response to NSAIDs and is responsible for some of the clinical symptoms. The aim of this study is to analyze clinical association of functional polymorphisms in the genes coding for enzymes involved in histamine homeostasis with hypersensitivity response to NSAIDs. We studied a cohort of 442 unrelated Caucasian patients with hypersensitivity to NSAIDs. Patients who experienced three or more episodes with two or more different NSAIDs were included. If this requirement was not met diagnosis was established by challenge. A total of 414 healthy unrelated controls ethnically matched with patients and from the same geographic area were recruited. Analyses of the SNPs rs17740607, rs2073440, rs1801105, rs2052129, rs10156191, rs1049742 and rs1049793 in the HDC, HNMT and DAO genes were carried out by means of TaqMan assays. The detrimental DAO 16 Met allele (rs10156191), which causes decreased metabolic capacity, is overrepresented among patients with crossed-hypersensitivity to NSAIDs with an OR = 1.7 (95% CI = 1.3-2.1; Pc = 0.0003) with a gene-dose effect (P = 0.0001). The association was replicated in two populations from different geographic areas (Pc = 0.008 and Pc = 0.004, respectively).Conclusions and implicationsThe DAO polymorphism rs10156191 which causes impaired metabolism of circulating histamine is associated with the clinical response in crossed-hypersensitivity to NSAIDs and could be used as a biomarker of response. more...
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- 2012
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22. The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray.
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Arantxa Palacín, Luis A Rivas, Cristina Gómez-Casado, Jacobo Aguirre, Leticia Tordesillas, Joan Bartra, Carlos Blanco, Teresa Carrillo, Javier Cuesta-Herranz, José A Cumplido Bonny, Enrique Flores, Mar G García-Alvarez-Eire, Ignacio García-Nuñez, Francisco J Fernández, Pedro Gamboa, Rosa Muñoz, Rosa Sánchez-Monge, Maria Torres, Susana Varela Losada, Mayte Villalba, Francisco Vega, Victor Parro, Miguel Blanca, Gabriel Salcedo, and Araceli Díaz-Perales more...
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Medicine ,Science - Abstract
Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy. more...
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- 2012
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23. Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.
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Arantxa Palacín, Cristina Gómez-Casado, Luis A Rivas, Jacobo Aguirre, Leticia Tordesillas, Joan Bartra, Carlos Blanco, Teresa Carrillo, Javier Cuesta-Herranz, Consolación de Frutos, Genoveva García Alvarez-Eire, Francisco J Fernández, Pedro Gamboa, Rosa Muñoz, Rosa Sánchez-Monge, Sofía Sirvent, María J Torres, Susana Varela-Losada, Rosalía Rodríguez, Victor Parro, Miguel Blanca, Gabriel Salcedo, and Araceli Díaz-Perales more...
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Medicine ,Science - Abstract
The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens. more...
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- 2012
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24. Sensitisation to Pollen Allergens in Children and Adolescents of Different Ancestry Born and Living in the Same Area
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Maria Luisa Somoza, Natalia Pérez-Sánchez, Isabel Torres-Rojas, Laura Martín-Pedraza, Natalia Blanca-López, Laura Victorio Puche, Eva Abel Fernández González, José Damián López Sánchez, Javier Fernández-Sánchez, Enrique Fernández-Caldas, Mayte Villalba, Francisco Javier Ruano, José Antonio Cornejo-García, Gabriela Canto, and Miguel Blanca more...
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Pulmonary and Respiratory Medicine ,ancestry ,Journal of Asthma and Allergy ,respiratory allergy ,Immunology and Allergy ,children/adolescents ,olive tree pollen ,molecular components ,pollen sensitisation - Abstract
Maria Luisa Somoza1 *, Natalia Pérez-Sánchez2 *, Isabel Torres-Rojas,1 Laura MartÃn-Pedraza,3 Natalia Blanca-López,1 Laura Victorio Puche,4 Eva Abel Fernández González,5 José Damián López Sánchez,6 Javier Fernández-Sánchez,7,8 Enrique Fernández-Caldas,5,9 Mayte Villalba,10 Francisco Javier Ruano,1 José Antonio Cornejo-GarcÃa11 *, Gabriela Canto1,12 *, Miguel Blanca3 ⨚llergy Department, Infanta Leonor University Hospital, Madrid, Spain; 2Allergy Department, Hospital Regional Universitario de Málaga, Málaga-IBIMA (FIMABIS), Málaga, Spain; 3Allergy Department, Fundación para la Investigación e Innovación Biomédica (FIIB) de los Hospitales Universitarios Infanta Leonor y Sureste, Madrid, Spain; 4Allergy Department, Morales Meseguer General University Hospital, Murcia, Spain; 5R&D Department, Inmunotek Laboratories, Madrid, Spain; 6Allergy Department, Virgen de la Arrixaca Clinical University Hospital, Murcia, Spain; 7Allergy Department, General University Hospital of Alicante- ISABIAL, Alicante, Spain; 8Clinical Medicine Department, Miguel Hernandez University, Alicante, Spain; 9Division of Allergy and Immunology, University of South Florida College of Medicine, Tampa, FL, USA; 10Department of Biochemistry and Molecular Biology, Universidad Complutense de Madrid, Madrid, Spain; 11Instituto de Investigación Biomédica de Málaga-IBIMA (FIMABIS), Málaga, Spain; 12School of Medicine, Universidad Complutense de Madrid, Madrid, Spain*These authors contributed equally to this workCorrespondence: Maria Luisa Somoza, Email mlsomoza@yahoo.comBackground: Allergy can start at early ages, with genetic and environmental factors contributing to its development.Aim: The study aimed to describe the pattern of sensitisation and allergy in children and adolescents of Spanish versus Moroccan ancestry but born in the same rural area of Spain.Methods: Participants were children and adolescents (3â 19 years) of Spanish or Moroccan descent, born in Blanca, Murcia (Spain). A detailed questionnaire was completed, and skin prick tests were performed to assess reactions to the most prevalent pollen allergens (O. europaea, P. pratense, S. kali, C. arizonica, P. acerifolia, A. vulgaris and P. judaica) plus molecular components Ole e 1 and Ole e 7. The association with ancestry was verified by studying participantsâ parents.Results: The study included 693 participants: 48% were aged 3â 9 years and 52%, 10â 19 years; 80% were of Spanish descent and 20% of Moroccan descent. Sensitisation to Olea europaea, Phleum pratense, Salsola kali and Cupressus arizonica were slightly higher in the Spanish group. The only significant differences were observed in sensitisation to Ole e 1 (p=0.02). Rhinitis, conjunctivitis, and rhinitis plus asthma were significantly higher in the Spanish group (p=0.03, p=0.02, p=0.007, respectively). The sensitisation pattern differed between Spanish and Moroccan parents, and between Moroccan parents and their children, but not between Spanish parents and their children.Conclusion: Both environment and ancestry may influence sensitisation and symptoms. Although the environment seems to have a stronger influence, other factors may contribute to the differences in prevalence and in the clinical entities in people of Spanish versus Moroccan descent.Keywords: ancestry, pollen sensitisation, children/adolescents, olive tree pollen, molecular components, respiratory allergy more...
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- 2022
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25. Weighted Epistatic Analysis of NSAIDs Hypersensitivity Data.
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Alex Upton, Miguel Blanca, José Antonio Cornejo-García, and James Richard Perkins
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- 2017
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26. Identifying Drone Web Sites in Multiple Countries and Languages with a Single Model
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Daas, Piet, de Miguel, Blanca, de Miguel, Maria, Daas, Piet, de Miguel, Blanca, and de Miguel, Maria
- Abstract
A text-based, bag-of-words, model was developed to identify drone company websites for multiple European countries in different languages. A collection of Spanish drone and non-drone websites was used for initial model development. Various classification methods were compared. Supervised logistic regression (L2-norm) performed best with an accuracy of 87% on the unseen test set. The accuracy of the later model improved to 88% when it was trained on texts in which all Spanish words were translated into English. Retraining the model on texts in which all typical Spanish words, such as names of cities and regions, and words indicative for specific periods in time, such as the months of the year and days of the week, were removed did not affect the overall performance of the model and made it more generally applicable. Applying the cleaned, completely English word-based, model to a collection of Irish and Italian drone and non-drone websites revealed, after manual inspection, that it was able to detect drone websites in those countries with an accuracy of 82 and 86%, respectively. The classification of Italian texts required the creation of a translation list in which all 1560 English word-based features in the model were translated to their Italian analogs. Because the model had a very high recall, 93, 100, and 97% on Spanish, Irish and Italian drone websites respectively, it was particularly well suited to select potential drone websites in large collections of websites. more...
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- 2023
27. Characteristics Of Peach Allergy In Madrid
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Rojas, Isabel Torres, primary, Pedraza, Laura Martín, additional, Haroun-Diaz, Elisa, additional, Vazquez de La Torre, Maria, additional, Puche, Laura Victorio, additional, Fernández Caldas, Enrique Enrique, additional, López Sánchez, José Damián, additional, Ruano, Francisco Javier, additional, Esteban Rodriguez, Ángel, additional, Gómez, Miguel Blanca, additional, and Somoza Alvarez, María Luisa, additional more...
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- 2023
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28. Quantitative genetic analysis of floral traits shows current limits but potential evolution in the wild
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Maria Clara Castellanos, Javier Montero-Pau, Peio Ziarsolo, Jose Miguel Blanca, Joaquin Cañizares, and Juli G. Pausas
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General Immunology and Microbiology ,Microbiologia ,General Medicine ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology ,Ecologia ,General Environmental Science - Abstract
The vast variation in floral traits across angiosperms is often interpreted as the result of adaptation to pollinators. However, studies in wild populations often find no evidence of pollinator-mediated selection on flowers. Evolutionary theory predicts this could be the outcome of periods of stasis under stable conditions, followed by shorter periods of pollinator change that provide selection for innovative phenotypes. We asked if periods of stasis are caused by stabilizing selection, absence of other forms of selection or by low trait ability to respond even if selection is present. We studied a plant predominantly pollinated by one bee species across its range. We measured heritability and evolvability of traits, using genome-wide relatedness in a large wild population, and combined this with estimates of selection on the same individuals. We found evidence for both stabilizing selection and low trait heritability as potential explanations for stasis in flowers. The area of the standard petal is under stabilizing selection, but the variability is not heritable. A separate trait, floral weight, presents high heritability, but is not currently under selection. We show how a simple pollination environment coincides with the absence of current prerequisites for adaptive evolutionary change, while heritable variation remains to respond to future selection pressures. more...
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- 2023
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29. A Cloud-based GWAS Analysis Pipeline for Clinical Researchers.
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Paul Heinzlreiter, James Richard Perkins, óscar Torreño Tirado, Tor Johan Mikael Karlsson, Juan Antonio Ranea, Andreas Mitterecker, Miguel Blanca, and Oswaldo Trelles
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- 2014
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30. Identifying Drone Web Sites in Multiple Countries and Languages with a Single Model
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Daas, Piet, primary, de Miguel, Blanca, additional, and de Miguel, Maria, additional
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- 2023
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31. Skin Testing With Peach Peel Extract Versus Serum IgE to Pru p 3 as a Stronger Predictor of Peach-Induced Anaphylaxis
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José Luis Subiza Garrido-Lestache, Miriam Morán Morales, Laura Victorio Puche, Abel Eva Fernández González, Angel Esteban Rodríguez, Natalia Blanca-López, Maria Luisa Somoza, Gabriela Canto, Ana María Prieto-Moreno Pfeifer, Laura Martín-Pedraza, Miguel Blanca, Enrique Fernández-Caldas, Jose Damian Lopez Sanchez, and Francisco Javier Fernández-Sánchez more...
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Pulmonary and Respiratory Medicine ,Intoxicative inhalant ,food hypersensitivity ,medicine.medical_specialty ,Allergy ,Immunology ,Immunoglobulin E ,medicine.disease_cause ,Brief Communication ,Gastroenterology ,skin tests ,Serum ige ,immunoglobulin E ,Allergen ,Internal medicine ,medicine ,anaphylaxis ,Immunology and Allergy ,Platanus acerifolia ,Artemisia vulgaris ,biology ,business.industry ,fruit ,medicine.disease ,biology.organism_classification ,Peach ,allergy ,pollen ,biology.protein ,business ,Anaphylaxis - Abstract
The most important peach fruit allergen is Pru p 3, followed by Pru p 1, Pru p 4, and Pru p 7. We aimed to assess their role in subjects with peach fruit-induced allergy (anaphylaxis and OAS) and compare skin prick tests (SPT) vs. specific immunoglobulin E (sIgE) for predicting anaphylaxis. We also selected a control group. SPT included prevalent inhalant and plant food allergens plus peach peel extract. The sIgE to Pru p 1, Pru p 3, Pru p 4, and Pru p 7 were quantified. Compared with controls (n = 42), cases (n = 41) were younger (P = 0.003), more frequently female (P < 0.05) and had higher SPT positivity to peach peel (44% vs. 2.4%, P < 0.0001). There were significant differences in sensitization to several pollens: Olea europaea, Artemisia vulgaris, Prunus persica, Platanus acerifolia (all P < 0.001); and fruits: apple (P < 0.04), peanut (P < 0.002), tomato (P < 0.005), and melon (P < 0.05). Pru p 3 sIgE was detected in 61% of all cases (85% anaphylaxis and 38% OAS; P < 0.01 each) and 5% of controls (P < 0.001). Pru p 4 sIgE was present in 19% of cases and 7% of controls. The sIgE to Pru p 1 and Pru p 7 were not found. The odds ratio to predict anaphylaxis for peach peel SPT was 113 (confidence interval [CI], 20-613; P < 0.0001); for sIgE to Pru p 3, 22 (CI, 5.3-93; P < 0.0001); and for SPT positivity to selected plant food allergens, 5 (CI, 1-19; P < 0.05). In our study group, SPT with peel peach extract was a better predictor of anaphylaxis than Pru p 3 sIgE or other variables considered. The role of sIgE to Pru p 1, Pru p 4, and Pru p 7 seemed negligible. more...
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- 2021
32. Lipid Transfer Protein Sensitization: Risk of Anaphylaxis and Molecular Sensitization Profile in Pru p 3-Sensitized Patients
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Javier Fernández-Sánchez, Antonio Palazón-Bru, Maria Ruano-Zaragoza, Maria Luisa Somoza, Victor Soriano-Gomis, Angel Esteban-Rodriguez, Teodorikez Wilfox Jimenez-Rodriguez, Purificación Gonzalez-Delgado, and Miguel Blanca more...
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Adult ,Male ,Allergy ,Adolescent ,Immunology ,Immunoglobulin E ,medicine.disease_cause ,Risk Assessment ,Diagnosis, Differential ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Allergen ,Oral allergy syndrome ,Risk Factors ,Food allergy ,medicine ,Humans ,Immunology and Allergy ,030223 otorhinolaryngology ,Anaphylaxis ,Sensitization ,Aged ,Plant Proteins ,Asthma ,biology ,business.industry ,Incidence ,General Medicine ,Antigens, Plant ,Middle Aged ,medicine.disease ,Cross-Sectional Studies ,medicine.anatomical_structure ,030228 respiratory system ,biology.protein ,Female ,Carrier Proteins ,business - Abstract
Background: Component-resolved diagnosis reveals the IgE response to many inhaled, food, and other allergens, improving the understanding and diagnosis of allergic diseases. Objective: The aims of the study are to study the recognition of different lipid transfer proteins (LTPs) and other allergen families in a large group of people sensitized to Pru p 3 and to analyze the relationship between the clinical entities and the allergens. Methods: This cross-sectional study included a large cohort of patients with positive skin tests to peach fruit and Pru p 3 specific IgE antibodies. Respiratory and food allergy symptoms were collected, and we performed prick tests with pollen, plant food, and other allergens plus the ImmunoCAP ISAC assay. Results: Our sample consisted of 421 people with a mean age of 33.25 years (range 16–68); 54.6% were women. Clinical entities included anaphylaxis (37.1%), urticaria (67.9%), and oral allergy syndrome (59.1%). Rhinitis, rhinoconjunctivitis, and/or asthma were diagnosed in 71.8% of the participants. The most pronounced correlation existed between sensitization to Pru p 3 and to Jug r 3, Pla a 3, Ara h 9, and Cor a 8. We found a higher incidence of anaphylaxis in people with 5 or more recognized LTPs. No association was observed between inhaled and food allergies. Conclusion: Most Pru p 3-sensitized participants were sensitized to additional allergens from the same family and, to a lesser extent, to other allergens, mainly in the profilin and PR-10 protein families. Anaphylaxis occurred in more than a third of the cases evaluated, and almost three-quarters of them had respiratory symptoms. Respiratory and food allergies involving LTPs do not seem to be associated. more...
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- 2020
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33. Pru p 10: the polygalacturonase described as a new peach tree pollen allergen
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Laura Martín-Pedraza, Andrea Wangorsch, Stephan Scheurer, Isabel Torres Rojas, Elisa Haroun-Diaz, Maria Vazquez de La Torre, Laura Victorio Puche, Enrique Fernandez-Caldas, Jose Luis Subiza, Natalia Blanca-Lopez, Miguel Blanca, and María Luisa Somoza more...
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Immunology ,Immunology and Allergy - Published
- 2023
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34. Characteristics Of Peach Allergy In Madrid
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Isabel Torres Rojas, Laura Martín Pedraza, Elisa Haroun-Diaz, Maria Vazquez de La Torre, Laura Victorio Puche, Enrique Enrique Fernández Caldas, José Damián López Sánchez, Francisco Javier Ruano, Ángel Esteban Rodriguez, Miguel Blanca Gómez, and María Luisa Somoza Alvarez more...
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Immunology ,Immunology and Allergy - Published
- 2023
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35. Dissolved iron equilibrium in bacterial leaching systems
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Wiertz, J. V., Ríos, Inés Godoy, and Miguel, Blanca Escobar
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- 1994
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36. Clavulanic Acid Is a Leading Culprit Beta-Lactam in Immediate Allergic Reactions to Penicillins
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Isabel Torres-Rojas, Diana Pérez-Alzate, Maria Luisa Somoza, Ana Prieto-Moreno Pfeifer, Elisa Haroun Diaz, Teodorikez W Jimenez-Rodriguez, Javier Fernández Sánchez, Francisco J Ruano, Miguel Blanca, and Natalia Blanca-López more...
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Pulmonary and Respiratory Medicine ,Immunology ,Immunology and Allergy - Published
- 2023
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37. Prunus persica9, a new occupational allergen from peach tree pollen involved in rhinitis and asthma
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Laura Martín-Pedraza, José Damian López-Sánchez, Enrique Fernández-Caldas, Laura Victorio-Puche, Maria Luisa Somoza, Eva Abel Fernandez, José Luis Subiza, Miguel Blanca, Mayte Villalba, and Mirian Moran more...
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0301 basic medicine ,Allergy ,biology ,business.industry ,medicine.medical_treatment ,Public Health, Environmental and Occupational Health ,medicine.disease_cause ,medicine.disease ,Immunoglobulin E ,Nasal provocation test ,03 medical and health sciences ,Prunus ,030104 developmental biology ,0302 clinical medicine ,Allergen ,030228 respiratory system ,Pollen ,Immunology ,medicine ,biology.protein ,business ,Occupational asthma ,Asthma - Abstract
ObjectivesSeveral studies have described peach tree (PT) as an occupational allergen. The aim of this work was to assess the effect ofPrunus persica9 (Pru p 9), a recently identified allergen from PT pollen, in exposed workers.MethodsThe study included people who reported respiratory symptoms after handling PT in orchards during the flowering period (Blanca village, Murcia region, south-east Spain). After completing a detailed questionnaire, participants underwent skin prick test (SPT) and nasal provocation test (NPT). The IgE response was analysed by SDS-PAGE and immunoblotting assays.ResultsA total of 21 cases were included (mean age 45 years; 57% women). Most were polysensitised to common pollens, although one person was sensitised only to PT pollen. All cases had a positive SPT to this pollen, and 43% also to Pru p 9. All participants reported having rhinitis, and six participants reported having also asthma. Immunoblotting showed a heterogeneous IgE pattern for several proteins, with Pru p 9 recognised in nine cases. Most participants sensitised to PT pollen and Pru p 9 had positive NPTs, while those who were not sensitised to Pru p 9 tested negative.ConclusionsWe demonstrate for the first time that Pru p 9, an allergen from PT pollen, can induce respiratory symptoms following occupational exposure. This must be considered a relevant allergen when people working with PT cultivars develop respiratory symptoms. more...
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- 2020
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38. Allergological Study of 565 Elderly Patients Previously Labeled as Allergic to Penicillins
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Maria Ruano-Zaragoza, Miguel Blanca, Natalia Blanca-López, Angel Esteban-Rodriguez, Antonio Palazón-Bru, José Manuel Ramos-Rincón, Teodorikez Wilfox Jimenez-Rodriguez, Javier Fernández-Sánchez, Geronima Riera-Sendra, and Victor Soriano-Gomis more...
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Allergy ,biology ,medicine.drug_class ,business.industry ,Antibiotics ,Drug allergy ,medicine.disease ,Immunoglobulin E ,Group B ,medicine.anatomical_structure ,Internal medicine ,medicine ,biology.protein ,Immunology and Allergy ,Clinical significance ,business ,Anaphylaxis ,Sensitization - Abstract
Purpose Elderly people thought to have an allergy to beta-lactams (BLs) may tolerate the drugs in subsequent exposures due to initial false labeling of allergies, the spontaneous loss of sensitivity to BLs over time or age-related decline in sensitization. As a result, they may be treated with less appropriate antibiotics, causing more side effects and entailing increased costs for health systems. The aim of this investigation was to assess whether patients in the third and fourth age with previously confirmed allergies to BLs had lost sensitization and could tolerate these antibiotics. Patients and methods Patients allergic to BLs were divided into group A (aged 60-79 years) and B (aged ≥80 years). Clinical history, skin testing, drug challenge tests (DCT) and evaluation of resensitization were used to classify participants as showing immediate reactions, non-immediate reactions, or tolerance. We compared clinical entities, drugs involved, and final outcome by age group. Results Of 1362 cases evaluated, 565 underwent an allergological study. The skin was the most common organ involved. Anaphylaxis and side chain reactions were more frequent in group A (p more...
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- 2019
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39. NSAID-induced reactions: classification, prevalence, impact, and management strategies
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Natalia, Blanca-Lopez, Victor, Soriano, Elena, Garcia-Martin, Gabriela, Canto, and Miguel, Blanca
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mechanisms ,NSAIDs ,hypersensitivity drug reactions ,Article ,management - Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs) are the leading cause of hypersensitivity drug reactions. The different chemical structures, cyclooxygenase 1 (COX-1) and/or COX-2 inhibitors, are taken at all ages and some can be easily obtained over the counter. Vasoactive inflammatory mediators like histamine and leukotriene metabolites can produce local/systemic effects. Responders can be selective (SR), IgE or T-cell mediated, or cross-intolerant (CI). Inhibition of the COX pathway is the common mechanism in CI, with the skin being the most frequent organ involved, followed by the lung and/or the nose. An important number of cases have skin and respiratory involvement, with systemic manifestations ranging from mild to severe anaphylaxis. Among SR, this is the most frequent entity, often being severe. Recent years have seen an increase in reactions involving the skin, with many cases having urticaria and/or angioedema in the absence of chronic urticaria. Aspirin, the classical drug involved, has now been replaced by other NSAIDs, with ibuprofen being the universal culprit. For CI, no in vivo/in vitro diagnostic methods exist and controlled administration is the only option unless the cases evaluated report repetitive and consistent episodes with different NSAIDs. In SR, skin testing (patch and intradermal) with 24–48 reading can be useful, mainly for delayed T-cell responses. Acetyl salicylic acid (ASA) is the test drug to establish the diagnosis and confirm/exclude CI by controlled administration. Desensitization to ASA has been extensively used in respiratory cases though it can also be applied in those cases where it is required. more...
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- 2019
40. Patterns of response and drugs involved in hypersensitivity reactions to beta-lactams in children
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Isabel Torres-Rojas, Gabriela Canto Díez, Elisa Haroun Diaz, Teodorikez Wilfox Jimenez-Rodriguez, Maria Luisa Somoza, Javier Fernandez Sánchez, Francisco Javier Ruano Pérez, Natalia Blanca-López, Ana María Prieto-Moreno Pfeifer, Miguel Blanca, and Diana Pérez-Alzate more...
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Drug ,Hypersensitivity, Immediate ,medicine.medical_specialty ,medicine.drug_class ,media_common.quotation_subject ,Immunology ,Provocation test ,Cephalosporin ,Immunoglobulin E ,beta-Lactams ,Drug Hypersensitivity ,Clavulanic acid ,Immunology and Allergy ,Medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Child ,media_common ,Skin Tests ,biology ,business.industry ,Amoxicillin ,Dermatology ,Anti-Bacterial Agents ,Penicillin ,Pharmaceutical Preparations ,Pediatrics, Perinatology and Child Health ,biology.protein ,business ,medicine.drug - Abstract
Background Beta-lactams generate different allergenic determinants that induce selective or cross-reactive drug hypersensitivity reactions (DHRs). We aimed to identify the drugs involved, the selectivity of the response, the mechanism, and the value of the different diagnostic tests for establishing a diagnosis in children evaluated for DHRs to beta-lactams. Methods Prospective study evaluating children aged under 16 years reporting DHRs to beta-lactams. Reactions were classified as immediate and nonimmediate reactions. The work-up included sIgE, skin testing and drug provocation tests (DPTs) for immediate reactions and patch testing and DPTs for nonimmediate ones. Results Of the 510 children included, 133 were evaluated for immediate reactions and confirmed in 8.3%. Skin test/in vitro IgE contributed to diagnosing half of the cases. Selective reactions occurred with amoxicillin (63%), followed by common penicillin determinants (27%) and cephalosporins (0.9%). Among nonimmediate reactions (11,4% of the 377 children evaluated), most required DPTs, 52.7% of which were positive at 6-7 days of drug challenge. Selective reactions were identified with amoxicillin (80%), penicillin G (7.5%), cephalosporins (7.5%), and clavulanic acid (5%). Urticaria and maculopapular exanthema were the most frequent entities. Conclusions There were few confirmed cases of either type of reaction. Skin testing proved less valuable in nonimmediate reactions, over half of which would also have been lost in a short DPT protocol. Selective responders to amoxicillin were more likely to have nonimmediate reactions, while clavulanic acid-selectivity was exclusive to the nonimmediate typology. Over half the cases with DPTs required 6-7 days of treatment for DHR confirmation. more...
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- 2021
41. Beta-lactam-induced immediate hypersensitivity reactions: A genome-wide association study of a deeply phenotyped cohort
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Neil H. Shear, Antonino Romano, Anca Mirela Chiriac, Matthew R. Nelson, Peter S. Friedmann, Alla Nakonechna, Rocco Luigi Valluzzi, S. Barrett, Jose A. Cornejo-Garcia, Miguel Blanca, Paola Nicoletti, Munir Pirmohamed, Daniel F. Carr, Pascal Demoly, Aris Floratos, Yufeng Shen, Natalia Blanca-López, Elizabeth J. Phillips, Cristiano Caruso, Laurence McEvoy, Francesco Gaeta, María José Torres, Rebecca Pavlos, Icahn School of Medicine at Mount Sinai [New York] (MSSM), SEMA4, University of Liverpool, University of Southampton, University of Toronto, Sunnybrook Health Sciences Centre, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Hospital Universitario Infanta Leonor [Madrid], Instituto de Investigación Biomédica [Malaga, Spain] (IBIMA), Fondazione Policlinico Universitario Agostino Gemelli IRCCS, IRCCS Ospedale Pediatrico Bambino Gesù [Roma], Columbia University [New York], The University of Western Australia (UWA), Vanderbilt University Medical Center [Nashville], Vanderbilt University [Nashville], Murdoch University, Epidemiology of Allergic and Respiratory Diseases Department [iPlesp] (EPAR), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut Desbrest de santé publique (IDESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Oasi Maria Santissima Srl [Troina, Italy], Hospital Regional Universitario de Málaga = Regional University Hospital of Malaga [Spain], Herrada, Anthony, Fondazione Policlinico Universitario Agostino Gemelli [Rome], Université de Montpellier (UM), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Hospital Regional Universitario de Málaga [Spain] more...
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0301 basic medicine ,Hypersensitivity, Immediate ,Male ,Allergy ,Linkage disequilibrium ,BL, β-Lactam ,Genome-wide association study ,Cohort Studies ,0302 clinical medicine ,QTL, Quantitative trait loci ,Immunology and Allergy ,Medicine ,[SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology ,SNP, Single nucleotide polymorphism ,Middle Aged ,3. Good health ,Anti-Bacterial Agents ,Phenotype ,Delayed hypersensitivity ,penicillins ,030220 oncology & carcinogenesis ,Cohort ,Female ,Anaphylaxis, Drug Allergy, Urticaria, and Angioedema ,[SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology ,Adult ,medicine.medical_specialty ,Immunology ,Penicillins ,Polymorphism, Single Nucleotide ,HLA-DQ alpha-Chains ,Drug Hypersensitivity ,03 medical and health sciences ,Internal medicine ,anaphylaxis ,Humans ,Genetic Predisposition to Disease ,Risk factor ,pharmacogenomics ,business.industry ,β-lactams ,Odds ratio ,medicine.disease ,allergy ,Cephalosporins ,030104 developmental biology ,cephalosporins ,business ,Type I hypersensitivity ,OR, Odds ratio ,Genome-Wide Association Study ,HLA-DRB1 Chains - Abstract
International audience; Background: β-lactam antibiotics are associated with a variety of immune-mediated or hypersensitivity reactions, including immediate (type I) reactions mediated by antigen-specific IgE.Objective: We sought to identify genetic predisposing factors for immediate reactions to β-lactam antibiotics.Methods: Patients with a clinical history of immediate hypersensitivity reactions to either penicillins or cephalosporins, which were immunologically confirmed, were recruited from allergy clinics. A genome-wide association study was conducted on 662 patients (the discovery cohort) with a diagnosis of immediate hypersensitivity and the main finding was replicated in a cohort of 98 Spanish cases, recruited using the same diagnostic criteria as the discovery cohort.Results: Genome-wide association study identified rs71542416 within the Class II HLA region as the top hit (P = 2 × 10-14); this was in linkage disequilibrium with HLA-DRB1∗10:01 (odds ratio, 2.93; P = 5.4 × 10-7) and HLA-DQA1∗01:05 (odds ratio, 2.93, P = 5.4 × 10-7). Haplotype analysis identified that HLA-DRB1∗10:01 was a risk factor even without the HLA-DQA1∗01:05 allele. The association with HLA-DRB1∗10:01 was replicated in another cohort, with the meta-analysis of the discovery and replication cohorts showing that HLA-DRB1∗10:01 increased the risk of immediate hypersensitivity at a genome-wide level (odds ratio, 2.96; P = 4.1 × 10-9). No association with HLA-DRB1∗10:01 was identified in 268 patients with delayed hypersensitivity reactions to β-lactams.Conclusions: HLA-DRB1∗10:01 predisposed to immediate hypersensitivity reactions to penicillins. Further work to identify other predisposing HLA and non-HLA loci is required. more...
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- 2021
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42. Genetic variants associated with T cell–mediated cutaneous adverse drug reactions: a PRISMA‐compliant systematic review—An EAACI position paper
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M. Gotua, Pascal Demoly, Marina Atanaskovic-Markovic, Knut Brockow, Munir Pirmohamed, Antonino Romano, Josefina Cernadas, Vincent Yip, Cristobalina Mayorga, Andreas J. Bircher, Abderrahim Oussalah, Jean-Christoph Roger J-P Caubet, Ingrid Terreehorst, Annick Barbaud, Jose Julio Laguna, Jean-Louis Guéant, Luciana Kase-Tanno, Miguel Blanca, Alla Nakonechna, Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Biochimie – Biologie moléculaire et Nutrition [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), University of Liverpool, Royal Liverpool and Broadgreen University Hospital NHS Trust, Institute of Translational Medicine, University of Liverpool, Instituto de Investigación Biomédica [Malaga, Spain] (IBIMA), Allergy Unit [Malaga, Spain] (National Network ARADyAL), Hospital Regional Universitario de Málaga [Spain], Service de dermatologie et allergologie [CHU Tenon], CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hospital de São João [Porto], Hospital Lusíadas Porto, Tbilisi State University, Technische Universität München [München] (TUM), Geneva University Hospitals and Geneva University, University Hospital Basel [Basel], University of Belgrade [Belgrade], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Hospital Sírio-Libanês [São Paulo, Brazil], Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA), Hospital Central de la Cruz Roja San Jose y Santa Adela, Clinica G.B. Morgagni (Fondazione Mediterranea), Ear, Nose and Throat, AII - Inflammatory diseases, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Salvy-Córdoba, Nathalie, Hospital Regional Universitario de Málaga = Regional University Hospital of Malaga [Spain], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Allergy Unit [Cruz Roja-Madrid], Faculté de Médecine [Nancy], Université de Lorraine (UL), Royal Liverpool University Hospital, and University of Liverpool-Royal Liverpool and Broadgreen University Hospital NHS Trust more...
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0301 basic medicine ,MESH: Carbamazepine ,MESH: Drug Hypersensitivity ,Genetic variants ,T-Lymphocytes ,Dapsone ,0302 clinical medicine ,systematic review ,Abacavir ,human leukocyte antigen genes ,Immunology and Allergy ,[SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology ,media_common ,ddc:618 ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences ,3. Good health ,[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences ,Carbamazepine ,Pharmaceutical Preparations ,Drug Hypersensitivity Syndrome ,T cell-mediated drug hypersensitivity reactions ,T cell–mediated drug hypersensitivity reactions ,[SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology ,medicine.drug ,Drug ,Nevirapine ,media_common.quotation_subject ,MESH: Pharmaceutical Preparations ,Immunology ,Human leukocyte antigen ,Drug Hypersensitivity ,03 medical and health sciences ,MESH: Drug Hypersensitivity Syndrome ,medicine ,Humans ,cutaneous adverse drug reactions ,MESH: Humans ,business.industry ,genetic variants ,Cutaneous adverse drug reactions ,[SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology ,medicine.disease ,Toxic epidermal necrolysis ,030104 developmental biology ,Human leukocyte antigen genes ,MESH: T-Lymphocytes ,030228 respiratory system ,HLA-B Antigens ,Stevens-Johnson Syndrome ,Systematic review ,MESH: HLA-B Antigens ,MESH: Stevens-Johnson Syndrome ,business ,Pharmacogenetics ,[SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology - Abstract
International audience; Drug hypersensitivity reactions (DHRs) are associated with high global morbidity and mortality. Cutaneous T cell-mediated reactions classically occur more than 6 hours after drug administration and include life-threatening conditions such as toxic epidermal necrolysis, Stevens-Johnson syndrome, and hypersensitivity syndrome. Over the last 20 years, significant advances have been made in our understanding of the pathogenesis of DHRs with the identification of human leukocyte antigens as predisposing factors. This has led to the development of pharmacogenetic screening tests, such as HLA-B*57:01 in abacavir therapy, which has successfully reduced the incidence of abacavir hypersensitivity reactions. We have completed a PRISMA-compliant systematic review to identify genetic associations that have been reported in DHRs. In total, 105 studies (5554 cases and 123 548 controls) have been included in the review reporting genetic associations with carbamazepine (n = 31), other aromatic antiepileptic drugs (n = 24), abacavir (n = 11), nevirapine (n = 14), trimethoprim-sulfamethoxazole (n = 11), dapsone (n = 4), allopurinol (n = 10), and other drugs (n = 5). The most commonly reported genetic variants associated with DHRs are located in human leukocyte antigen genes and genes involved in drug metabolism pathways. Increasing our understanding of genetic variants that contribute to DHRs will allow us to improve diagnosis, develop new treatments, and predict and prevent DHRs in the future. more...
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- 2020
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43. Peach Tree Pollen and Prunus persica 9 Sensitisation and Allergy in Children and Adolescents
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María Garrido-Arandia, José Damian López-Sánchez, Laura Victorio Puche, Araceli Díaz-Perales, Miguel Blanca, and Maria Luisa Somoza
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Allergy ,medicine.medical_specialty ,medicine.medical_treatment ,Immunology ,medicine.disease_cause ,Nasal provocation test ,Phleum ,03 medical and health sciences ,Prunus ,0302 clinical medicine ,Allergen ,Internal medicine ,medicine ,Immunology and Allergy ,Clinical significance ,030223 otorhinolaryngology ,biology ,business.industry ,General Medicine ,medicine.disease ,biology.organism_classification ,030228 respiratory system ,Large group ,business ,Tree pollen - Abstract
Background: Although plant and fruit pollens are entomophilous and relevant in exposed workers, we have shown a high frequency of sensitisation and symptoms induction of peach tree pollen (PTP) and Prunus persica 9 (Pru p 9) in adults from areas of peach cultivars. Methods: We studied the sensitisation and clinical relevance of PTP and Pru p 9 in a large group of children and adolescents aged 3–19 years. A detailed questionnaire plus skin prick testing to prevalent allergens, PTP, and Pru p 9 were carried out. The clinical relevance was established by nasal provocation test (NPT) and symptom score index. Results: We evaluated 685 children (mean age 8.75 ± 3.3 years, median 9 years), 52% of them female. Sensitisation to PTP occurred in 20% of the cases following olive tree (33%) and Phleum pratense (26%). In a randomly selected subgroup of subjects sensitised to PTP, 30% were skin prick test-positive to Pru p 9. Most cases had rhinitis or rhinoconjunctivitis. NPT showed the relevance of PTP and Pru p 9 in the induction of symptoms. Conclusion: PTP and Pru p 9 are relevant in the induction of sensitisation and respiratory symptoms in children and adolescents. This allergen should be evaluated in children living in regions of peach tree cultivars. more...
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- 2019
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44. THE ROLE OF CLAVULANIC ACID IN IMMEDIATE ALERGIC REACTIONS TO BETA-LACTAMS
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Natalia Blanca-López, Ana Maria Prieto-Moreno Pfeifer, Isabel Torres Rojas, Diana Pérez-Alzate, M Luisa Somoza, Elisa Haroun-Diaz, Maria Vazquez de La Torre, Paula Lopez, Maria D. Cervera Garcia, Carlos Alberto Blanco Mota, Francisco Javier Ruano, and Miguel Blanca more...
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Immunology ,Immunology and Allergy - Published
- 2022
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45. Allergenicity Analysis of Pru p 9 IgE-epitope: The First Peach Aeroallergen
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Laura Martín-Pedraza, Andrea Wangorsch, Natalia Perez Sanchez, Laura Luna, Annette Jamin, Enrique Fernandez-Caldas, Jose Luis Subiza, Jonas Lidholm, Elisa Haroun, María Vazquez de la Torre, Miguel Blanca, Jose Cornejo-Garcia, Stephan Scheurer, and Maria Luisa Somoza more...
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Immunology ,Immunology and Allergy - Published
- 2022
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46. NSAIDs hypersensitivity: questions not resolved
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Teresa Bellón, Miguel Blanca, Natalia Blanca-López, Gemma Amo, Maria Luisa Somoza-Alvarez, and Gabriela Canto
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Hypersensitivity, Immediate ,Pharmacogenomic Variants ,T cell ,Respiratory Tract Diseases ,Immunology ,Disease ,Immunologic Tests ,Bioinformatics ,Immunoglobulin E ,Drug Hypersensitivity ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,T-Lymphocyte Subsets ,Prevalence ,Humans ,Immunology and Allergy ,Medicine ,Angioedema ,skin and connective tissue diseases ,biology ,business.industry ,Anti-Inflammatory Agents, Non-Steroidal ,Respiratory disease ,Symptom Flare Up ,medicine.disease ,Acquired immune system ,Pharmacogenomic Testing ,medicine.anatomical_structure ,030228 respiratory system ,biology.protein ,medicine.symptom ,business ,Prostaglandin metabolism ,Anaphylaxis - Abstract
Purpose of review NSAIDs are the drugs most frequently involved in hypersensitivity reactions (HSR). These are frequently prescribed at all ages. HSR are of great concern and can affect people at any age. These drugs can induce reactions by stimulating the adaptive immune system (IgE or T cell), known as selective responders or more frequently by abnormalities in biochemical pathways related with prostaglandin metabolism. These are known as cross-intolerant. With some exceptions, skin testing and in-vitro studies are of little value in selective responders. Recent findings In the last years, several classifications have been provided based on clinical symptoms, time interval between drug intake and appearance of symptoms, response to other nonchemically related NSAIDs and the underlying disease. Based on this classification, several well differentiated categories within each group of entities cross-intolerant and selective responders are now recognized. The most complex groups for evaluation are cross-intolerant in which three major groups exist: NSAIDs exacerbated respiratory disease, NSAIDs exacerbated cutaneous disease and NSAIDs-induced urticaria/angioedema in the absence of chronic spontaneous urticaria. Within the selective responders, there are two mechanisms involved: drug-specific IgE or T-cell effector responses. New entities have been added to this classification like mixed reactions within the cross-intolerant category, that must manifest as anaphylaxis and multiple immediate selective reactions. Summary The precise evaluation of patients with NSAIDs hypersensitivity following established guidelines will improve not only our understanding but also the management of these entities. As the number of patients affected with NSAIDs is important, further studies are warranted. more...
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- 2018
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47. Acetyl Salicylic Acid Challenge in Children with Hypersensitivity Reactions to Nonsteroidal Anti-Inflammatory Drugs Differentiates Between Cross-Intolerant and Selective Responders
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Natalia Blanca-López, Maria Vázquez de La Torre Gaspar, Miguel Blanca, Diana Pérez-Alzate, Elena García-Martín, Maria Luisa Somoza, Francisco Javier Ruano, Francisco Rivas-Ruiz, Elisa Haroun-Díaz, and Gabriela Canto more...
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Male ,Drug ,medicine.medical_specialty ,Allergy ,Adolescent ,medicine.drug_class ,media_common.quotation_subject ,Administration, Oral ,Ibuprofen ,Pharmacology ,Culprit ,Anti-inflammatory ,Drug Hypersensitivity ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Interquartile range ,Immune Tolerance ,medicine ,Humans ,Immunology and Allergy ,Single-Blind Method ,030212 general & internal medicine ,Child ,Acetaminophen ,media_common ,Nonsteroidal ,Aspirin ,business.industry ,Anti-Inflammatory Agents, Non-Steroidal ,Infant ,medicine.disease ,Dermatology ,030228 respiratory system ,chemistry ,Child, Preschool ,Female ,business ,Salicylic acid ,medicine.drug - Abstract
Background Hypersensitivity reactions to nonsteroidal anti-inflammatory drugs (NSAIDs) in children are becoming a great concern. Most studies have focused on adults, with noted discrepancies observed in the classification of hypersensitivity reactions to NSAIDs in children when compared with adults. Objective To phenotype a group of children with hypersensitivity reactions to NSAIDs, including paracetamol, and analyze the degree of agreement with the entities reported in adults and how they fit the proposed classifications. Methods The study comprised 116 children aged 0.5 to 14 years, with a clinical history indicative of hypersensitivity reactions to NSAIDs. They all underwent a single-blind oral provocation test with acetyl salicylic acid, except in those cases when this was the suspected drug, in which case the challenge was done first with ibuprofen. If positive, cross-intolerance was established and if negative, an oral provocation test with the culprit drug was performed to establish a selective response or exclude allergy. Results Of the 26% diagnosed as hypersensitive to NSAIDs, 83% were cross-intolerant and 17% selective reactors. The highest significant differences between reactors and nonreactors were observed in the time to reaction after drug intake and the clinical entity (P Conclusions From the total number of cases confirmed with NSAID hypersensitivity, 83% were cross-intolerant. In cross-intolerant reactions, both cutaneous and respiratory manifestations are common. Acetyl salicylic acid challenge as the first approach proved to be safe and useful to establish the diagnosis. more...
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- 2018
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48. Editorial: Advances in drug hypersensitivity reactions
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Miguel Blanca and Paul Whitaker
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Drug ,medicine.medical_specialty ,business.industry ,T-Lymphocytes ,media_common.quotation_subject ,Anti-Inflammatory Agents, Non-Steroidal ,Immunology ,MEDLINE ,Allergens ,Immunoglobulin E ,Drug Hypersensitivity ,Text mining ,HLA Antigens ,medicine ,Animals ,Humans ,Immunology and Allergy ,Genetic Predisposition to Disease ,Immunization ,business ,Intensive care medicine ,media_common - Published
- 2019
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49. Epidemiology of Hymenoptera venom allergy in the Valencia Fire Brigade (Spain)
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Soriano-Gomis, Victor, primary, Cabrera-Beyrouti, Ruben, additional, Serrano-Delgado, Pilar, additional, Jimenez-Rodriguez, Teodorikez Wilfox, additional, Gómez, Miguel Blanca, additional, and Fernandez-Sanchez, Javier, additional more...
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- 2021
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50. ART THINKING AS A CATALYST FOR STEAM STRATEGIES. TOWARDS A DIVERGENT THINKING
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Santamarina, Virginia, primary, de-Miguel-Molina, María, additional, De-Miguel, Blanca, additional, and Carabal, Mª Ángeles, additional
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- 2020
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