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Your search keyword '"Mihelich, Nicole T."' showing total 9 results
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9 results on '"Mihelich, Nicole T."'

1. Crabgrass weed suppression in fine fescues

Author

Breuillin-Sessoms, Florence, Petrella, Dominic P., Trappe, Jon M., Mihelich, Nicole T., Patton, Aaron J., and Watkins, Eric

Subjects
Business, Sports, sporting goods and toys industry
Abstract

Fine fescues, a group of low-input turfgrasses, have exhibited weed suppression. Smooth crabgrass is a ubiquitous, warm-season, grassy weed that outcompetes many cool-season turfgrasses in summer (9). The presence of [...]

Published
2021

9. Genome Editing in Soybean with CRISPR/Cas9.

Author

Liu J, Gunapati S, Mihelich NT, Stec AO, Michno JM, and Stupar RM

Subjects
Electrophoresis, Polyacrylamide Gel, Plant Roots genetics, Transformation, Genetic genetics, CRISPR-Cas Systems genetics, Gene Editing methods, Plants, Genetically Modified genetics, Glycine max genetics
Abstract

CRISPR/Cas9 mediated genome editing technology has experienced rapid advances in recent years and has been applied to a wide variety of plant species, including soybean. Several platforms have been developed for designing and cloning of single CRISPR targets or multiple targets in a single destination vector. This chapter provides an updated working protocol for applying CRISPR/Cas9 technology to target a single gene or multiple genes simultaneously in soybean. We describe two platforms for cloning single CRISPR targets and multiplexing targets, respectively, and reagent delivery methodologies. The protocols address crucial limiting steps that can limit CRISPR editing in soybean hairy roots, composite plants, and tissue culture-based regenerated whole plants. To date, transgenic soybean plants with mutagenesis in up to three target genes have been obtained with this procedure.

Published
2019
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