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1. Non-biogroup 1 or 2 Strains of the Emerging Zoonotic Pathogen Escherichia albertii, Their Proposed Assignment to Biogroup 3, and Their Commonly Detected Characteristics

Author

Koichi Murakami, Eriko Maeda-Mitani, Hirokazu Kimura, Mikiko Honda, Tetsuya Ikeda, Wakana Sugitani, Takayuki Konno, Kimiko Kawano, Yoshiki Etoh, Nobuyuki Sera, Fuminori Mizukoshi, Takehito Saitoh, Yoshiaki Kawamura, Taisei Ishioka, Makoto Ohnishi, Kazunori Oishi, and Shuji Fujimoto

Subjects
biogroups, Escherichia albertii, food microbiology, identification, public health, Microbiology, QR1-502
Abstract

Escherichia albertii, a zoonotic enteropathogen, is responsible for outbreaks of disease in humans. Identifying strains of E. albertii by phenotypic characterization tests is difficult because of its poorly defined properties. Screening its phenotypic characteristics is, nevertheless, a necessary prerequisite for further genetic analysis of its properties, and species-specific polymerase chain reaction (PCR) analysis can be used to type the pathogen. While two E. albertii biogroups (1 and 2) have been described, strains with characteristics divergent from both biogroups have been reported worldwide. The aim of the present study was to evaluate the characteristics of non-biogroup 1 or 2 strains, and discern the characteristics common to all of the E. albertii strains from this study. Altogether, 107/414 field isolates were selected for examination based on pulsed-field gel electrophoresis analysis. The 107 strains were isolated from 92 sources, including humans and pigeon feces, other wild birds, and retail chicken livers. All strains were then examined using various culture-based, biochemical (API 50CHE tests, API Zym test, and others) and molecular (virulence gene screening, multi-locus sequence analysis) testing methods. Our results revealed that all field strains (n = 107) showed non-biogroup 1 or 2 characteristics, with multiple sequence differences. Variations in indole production and the lysine decarboxylase activity profiles among the isolates made identification of E. albertii very difficult. Therefore, we propose that non-biogroup 1 or 2 of E. albertii should be assigned to biogroup 3 to make screening of them easier in public health and clinical laboratory settings. Clearly, having group criteria for indole-negative/lysine-positive, indole-positive/lysine-negative, and indole-positive/lysine-positive E. albertii biogroups 1, 2, and 3 strains, respectively, should provide for more accurate identification of E. albertii isolates. Based on our findings, we recommend that isolates displaying phenotype mobility-negativity (sulfide-indole-motility medium, 37°C), hydrogen sulfide production-negativity (triple sugar iron medium), acid production-negativity from xylose, negative β-glucuronidase activity properties, and showing indole production and lysine decarboxylase activity profiles in accordance with one of the three biogroups, should be further assessed using an E. albertii-specific PCR assay.

Published
2019
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2. Epidemiological Aspects of Escherichia albertii Outbreaks in Japan and Genetic Characteristics of the Causative Pathogen

Author

Wakana Sugitani, Kazunori Oishi, Yukio Morita, Junko Toda, Takahiro Hiratsuka, Hiroko Akita, Makoto Kuroda, Taisei Ishioka, Hiroshi Narimatsu, Makoto Ohnishi, Mami Fukada, Kaori Inoue, Shinichiro Hirai, Koichi Murakami, Shuji Fujimoto, Tsuyoshi Sekizuka, Kanako Masuda, Tetsuya Hayashi, Shinichi Takao, Mikiko Honda, Hirokazu Kimura, and Tadasuke Ooka

Subjects
0303 health sciences, medicine.medical_specialty, biology, 030306 microbiology, 040301 veterinary sciences, General symptoms, Outbreak, macromolecular substances, 04 agricultural and veterinary sciences, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Incubation period, Escherichia albertii, 0403 veterinary science, 03 medical and health sciences, Diarrhea, Epidemiology, medicine, Animal Science and Zoology, medicine.symptom, Pathogen, Zoonotic pathogen, Food Science
Abstract

Zoonotic pathogen Escherichia albertii has been identified as the cause of several human disease outbreaks; however, factors such as the general symptoms and incubation period of E. albertii infect...

Published
2020
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3. O-antigen biosynthesis gene clusters of

Author

Tadasuke, Ooka, Kazuko, Seto, Yoshitoshi, Ogura, Keiji, Nakamura, Atsushi, Iguchi, Yasuhiro, Gotoh, Mikiko, Honda, Yoshiki, Etoh, Tetsuya, Ikeda, Wakana, Sugitani, Takayuki, Konno, Kimiko, Kawano, Naoko, Imuta, Kiyotaka, Yoshiie, Yukiko, Hara-Kudo, Koichi, Murakami, Tetsuya, Hayashi, and Junichiro, Nishi

Subjects
Escherichia, Base Sequence, Genotype, O Antigens, Systems Microbiology: Large-scale comparative genomics, phylogeny, Escherichia albertii, genotyping, Multigene Family, O-antigen gene cluster, Escherichia coli, Humans, Serotyping, genome, Genome, Bacterial, Research Article
Abstract

Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli . In many Gram-negative bacteria, including E. coli , O-antigen variation has long been used for the serotyping of strains. In E. albertii , while eight O-serotypes unique to this species have been identified, some strains have been shown to exhibit genetic or serological similarity to known E. coli / Shigella O-serotypes. However, the diversity of O-serotypes and O-antigen biosynthesis gene clusters (O-AGCs) of E. albertii remains to be systematically investigated. Here, we analysed the O-AGCs of 65 E. albertii strains and identified 40 E. albertii O-genotypes (EAOgs) (named EAOg1–EAOg40). Analyses of the 40 EAOgs revealed that as many as 20 EAOgs exhibited significant genetic and serological similarity to the O-AGCs of known E. coli / Shigella O-serotypes, and provided evidence for the inter-species horizontal gene transfer of O-AGCs between E. albertii and E. coli . Based on the sequence variation in the wzx gene among the 40 EAOgs, we developed a multiplex PCR-based O-genotyping system for E. albertii (EAO-genotyping PCR) and verified its usefulness by genotyping 278 E. albertii strains from various sources. Although 225 (80.9 %) of the 278 strains could be genotyped, 51 were not assigned to any of the 40 EAOgs, indicating that further analyses are required to better understand the diversity of O-AGCs in E. albertii and improve the EAO-genotyping PCR method. A phylogenetic view of E. albertii strains sequenced so far is also presented with the distribution of the 40 EAOgs, which provided multiple examples for the intra-species horizontal transfer of O-AGCs in E. albertii .

Published
2019

4. O-antigen biosynthesis gene clusters of Escherichia albertii: their diversity and similarity to Escherichia coli gene clusters and the development of an O-genotyping method

Author

Tetsuya Hayashi, Kazuko Seto, Mikiko Honda, Koichi Murakami, Keiji Nakamura, Junichiro Nishi, Wakana Sugitani, Kimiko Kawano, Yoshitoshi Ogura, Naoko Imuta, Atsushi Iguchi, Takayuki Konno, Yasuhiro Gotoh, Yukiko Hara-Kudo, Tadasuke Ooka, Yoshiki Etoh, Kiyotaka Yoshiie, and Tetsuya Ikeda

Subjects
Genetics, biology, Phylogenetic tree, General Medicine, medicine.disease_cause, biology.organism_classification, Genome, Escherichia albertii, Phylogenetics, Horizontal gene transfer, medicine, Shigella, Genotyping, Escherichia coli
Abstract

Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli . In many Gram-negative bacteria, including E. coli , O-antigen variation has long been used for the serotyping of strains. In E. albertii , while eight O-serotypes unique to this species have been identified, some strains have been shown to exhibit genetic or serological similarity to known E. coli / Shigella O-serotypes. However, the diversity of O-serotypes and O-antigen biosynthesis gene clusters (O-AGCs) of E. albertii remains to be systematically investigated. Here, we analysed the O-AGCs of 65 E. albertii strains and identified 40 E. albertii O-genotypes (EAOgs) (named EAOg1–EAOg40). Analyses of the 40 EAOgs revealed that as many as 20 EAOgs exhibited significant genetic and serological similarity to the O-AGCs of known E. coli / Shigella O-serotypes, and provided evidence for the inter-species horizontal gene transfer of O-AGCs between E. albertii and E. coli . Based on the sequence variation in the wzx gene among the 40 EAOgs, we developed a multiplex PCR-based O-genotyping system for E. albertii (EAO-genotyping PCR) and verified its usefulness by genotyping 278 E. albertii strains from various sources. Although 225 (80.9 %) of the 278 strains could be genotyped, 51 were not assigned to any of the 40 EAOgs, indicating that further analyses are required to better understand the diversity of O-AGCs in E. albertii and improve the EAO-genotyping PCR method. A phylogenetic view of E. albertii strains sequenced so far is also presented with the distribution of the 40 EAOgs, which provided multiple examples for the intra-species horizontal transfer of O-AGCs in E. albertii .

Published
2019
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5. Epidemiological Aspects of

Author

Kanako, Masuda, Tadasuke, Ooka, Hiroko, Akita, Takahiro, Hiratsuka, Shinichi, Takao, Mami, Fukada, Kaori, Inoue, Mikiko, Honda, Junko, Toda, Wakana, Sugitani, Hiroshi, Narimatsu, Taisei, Ishioka, Shinichiro, Hirai, Tsuyoshi, Sekizuka, Makoto, Kuroda, Yukio, Morita, Tetsuya, Hayashi, Hirokazu, Kimura, Kazunori, Oishi, Makoto, Ohnishi, Shuji, Fujimoto, and Koichi, Murakami

Subjects
Escherichia, Foodborne Diseases, Genotype, Japan, Virulence Factors, Waterborne Diseases, Enterobacteriaceae Infections, Type III Secretion Systems, Humans, Genome, Bacterial, Phylogeny, Disease Outbreaks
Abstract

Zoonotic pathogen

Published
2019

6. Does Sequence Type 33 of Shiga Toxin-Producing Escherichia coli O91 Cause Only Mild Symptoms?

Author

Nanami Asoshima, Koichi Murakami, Nobuyuki Sera, Shuji Fujimoto, Mikiko Honda, Masanori Watahiki, Daisuke Onozuka, Yoshiki Etoh, Sunao Iyoda, Eriko Maeda, and Hiroshi Narimatsu

Subjects
Diarrhea, Microbiology (medical), animal diseases, Biology, medicine.disease_cause, Microbiology, fluids and secretions, medicine, Humans, Letters to the Editor, Escherichia coli, Shiga toxin-producing Escherichia coli, Escherichia coli Infections, Phylogeny, Sequence (medicine), Shiga-Toxigenic Escherichia coli, Shiga toxin, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Abdominal Pain, Mild symptoms, biology.protein, bacteria, Multilocus sequence typing, medicine.symptom, Multilocus Sequence Typing
Abstract

The pattern of symptoms caused by Shiga toxin (Stx)-producing Escherichia coli (STEC) O91 in Japan differs from that in European countries. STEC O91 is often isolated from sporadic adult cases of hemolytic-uremic syndrome (HUS) in France ([1][1]), while in Germany, STEC O91 with sequence type 442 (

Published
2015
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8. The map-based sequence of the rice genome

Author

Shu Mei Liu, Hong-Hwa Chen, Kiran Kumar, Aki Iwabuchi, Luke J. Tallon, Yasuyuki Fujii, Yuichi Ito, Jennifer Currie, Douglas Fadrosh, Bruce Weaver, Hisakazu Iwama, Nahoko Fujitsuka, Arvind K. Bharti, Vivek Dalal, Eric Pelletier, Wataru Karasawa, Carol Soderlund, Masako Okamoto, Ajit K. Pal, Lei Zhang, Tomoko Maehara, Anupama Gaur, Tomotaro Nishikawa, Michiko Ikeda, Jingjie Zhu, Meizhong Luo, Yoshiharu Sato, Dibyendu Kumar, Mikiko Honda, Takuya Habara, Jianyu Song, Yuka Takazaki, Alok Singh, Ari Kikuta, Neilay Dedhia, Thomas E. Bureau, Eric Linton Victor Llaca, Nadia Demange, Yoshiaki Nagamura, Koh Ichi Kadowaki, Takanori Shimokawa, Kohei Arita, Patrick Wincker, Saori Hijishita, Kai Ying, Takahito Bito, Tae-Jin Yang, Mayu Yamamoto, Masahiro Yano, Paulo Dejalma Zimmer, Nagendra K. Singh, Atsuko Idonuma, Jia Liu, Anne Ciecko, Friedrich Engler, Shinji Naito, Pei Fang Lee, Hideki Nagasaki, Jianping Guan, Yoko Ichikawa, Sujit Dike, Shu Ouyang, Yuko Nakama, Masao Hamada, Saurabh Raghuvanshi, Ching San Chen, Teh Yuan Chow, Joseph Hsiao, Béatrice Segurens, Hiroaki Sakai, W. Richard McCombie, Nobukazu Namiki, Hiroyuki Kanamori, Mei-Chu Chung, Yiqi Lu, Claude Scarpelli, Kelly Moffat, Yoshino Chiden, Baltazar A. Antonio, Susan R. McCouch, Paramjit Khurana, Amy Bronzino Nelson, Tamara Feldblyum, Hiroshi Mizuno, Masatoshi Masukawa, Yoshihito Niimura, Tomoya Ohta, Joachim Messing, Yukiyo Ito, Lance E. Palmer, Antonio Costa de Oliveira, Nozomi Ono, Ai Ling Hour, Kumiko Tsuji, Qijun Weng, Michael W. Bevan, Guofan Hong, C. Robin Buell, Subodh K. Srivastava, Atul Bhargava, Galina Fuks, Masahiro Sugiura, Akio Miyao, Kristine Jones, John Yu Liou, Ayano Meguro, Aymeric R. De Vazeille, Mika Tsugane, Xiaohui Liu, Rie Fukunaka, Jean Weissenbach, Shu Jen Lin, Jayati Bera, Tomoya Baba, Yao-Cheng Lin, Lori Spiegel, Laurence Cattolico, Irfan Ahmad Ghazi, Shoko Saji, Jianzhong Wu, Danlin Fan, Takashi Gojobori, Rod A. Wing, Harumi Yamagata, Koji Arikawa, Shuliang Yu, Marcel Salanoubat, Yumi Nakamichi, Kristi Collura, Jetty S.S. Ammiraju, Vipin Gupta, Stephen I. Wright, Y. Huang, Qiang Zhao, Yuichi Katayose, Tingting Lu, Stacey E. Iobst, Akhilesh K. Tyagi, A. O'Shaughnessy, Tilak Raj Sharma, Hiroyoshi Aoki, Kazue Ito, Marina Nakashima, Vydianathan Ravi, J. F. Shaw, Takashi Matsumoto, Tamara Tsitrin, Yoshiyuki Mukai, Steve Reidmuller, Steve Young, Kozue Kamiya, Lidia Nascimento, Qi Feng, Shivani Johri, Kazuko Yukawa, Sylvie Samain, Hirohiko Hirochika, Kimiko Yamamoto, Matthew Reardon, Holly Cordum, Mu Kuei Chu, Kim Yul Ho, Victoria Zismann, Jessica Hill, Tomoko Ito, Gregory Wilson, Isamu Ohta, Bahattin Tanyolac, Amitabh Mohanty, Rie Yoshihara, Nikoleta Juretic, Bin Han, Jie Mu, Susan Van Aken, Satomi Hosokawa, Kayo Machita, Shaohua Jin, Odir Antônio Dellagostin, Satoshi Katagiri, Apichart Vanavichit, Takuji Sasaki, Douglas R. Hoen, Richard K. Wilson, Patrick Minx, Larry Overton, Rentao Song, Kimihiro Terasawa, Jang Ho Hahn, Steve Kavchok, Hiroko Yamane, Parul Khurana, Melissa De La Bastide, Gisela Orjeda, Francis Quetier, Katsumi Sakata, Anita Kapur, Hyeran Kim, Grace Pai, Ian Bancroft, Trilochan Mohapatra, Manami Negishi, Ya Ting Chao, Kanako Kurita, Mari Nakamura, Masaki Fujisawa, Maiko Ikeno, Yu Zhang, Miyuki Sakaguchi, Yuko Nagata, Harumi Kobayashi, Kamlesh Batra, Huisun Zhong, Mary Kim, T. Utterback, Shoji Yoshiki, A. Pandit, Chizuko Harada, Benjamin Burr, Jacqueline Jackson, Jitendra P. Khurana, Michie Shibata, Jiming Jiang, Hue Vuong, Chia Hsiung Cheng, Sachie Ito, Zhukuan Cheng, Qiaoping Yuan, Akiko Hayashi, Tatsumi Mizubayashi, Yeisoo Yu, Nathalie Choisne, Shubha Vij, Noriko Kobayashi, Vivekanand Balija, Hong Pang Wu, K. Sureshbabu, Ying Li, Theresa Zutavern, G. Sangsakoo, Kristen Gansberger, Yujun Zhang, Kazunori Waki, Weiwei Jin, R. Preston, Shigenori Ueda, Yilei Liu, Angélique D'Hont, Kwang-Jen Hsiao, Kumiko Sakai, Richard Bruskiewich, Luiz Anderson Teixeira de Mattos, Teri Rambo, Yue-Ie C. Hsing, Mahavir Yadav, Shinichi Yamamoto, Arnaud Couloux, Sally A. Leong, Kishor Gaikwad, Masumi Iijima, Takeshi Itoh, Gaspar Malone, Gladys Keizer, and Anupam Dixit

Subjects
Transposable element, Identification, RNA, Untranslated, Euchromatin, International Cooperation, Centromere, Molecular Sequence Data, Arabidopsis, Oryza sativa, Biology, Genes, Plant, Zea mays, Genome, Chromosomes, Plant, F30 - Génétique et amélioration des plantes, Gene family, Cloning, Molecular, Gene, Sorghum, Synteny, Cell Nucleus, Organelles, Genetics, Polymorphism, Genetic, Génome, Multidisciplinary, Computational Biology, food and beverages, Oryza, Genomics, Sequence Analysis, DNA, Tandem Repeat Sequences, Gène, Multigene Family, Proteome, DNA Transposable Elements, Carte génétique, Genome, Plant, Caractère agronomique
Abstract

Rice, one of the world's most important food plants, has important syntenic relationships with the other cereal species and is a model plant for the grasses. Here we present a map-based, finished quality sequence that covers 95% of the 389 Mb genome, including virtually all of the euchromatin and two complete centromeres. A total of 37,544 non-transposable-element-related protein-coding genes were identified, of which 71% had a putative homologue in Arabidopsis. In a reciprocal analysis, 90% of the Arabidopsis proteins had a putative homologue in the predicted rice proteome. Twenty-nine per cent of the 37,544 predicted genes appear in clustered gene families. The number and classes of transposable elements found in the rice genome are consistent with the expansion of syntenic regions in the maize and sorghum genomes. We find evidence for widespread and recurrent gene transfer from the organelles to the nuclear chromosomes. The map-based sequence has proven useful for the identification of genes underlying agronomic traits. The additional single-nucleotide polymorphisms and simple sequence repeats identified in our study should accelerate improvements in rice production.

Published
2005
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9. Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers

Author

Yuki Tokoi, Shannon D. Manning, Hiroshi Nakajima, Yuka Kojima, Atsushi Iguchi, Takashi Hatakeyama, Yuhki Nagai, Nanami Asoshima, Mitsuhiro Kameyama, Hiromi Nakamura, Hiroyuki Ueno, Kikuyo Ogata, Tomoko Morita-Ishihara, Shinya Kawanishi, Yuji Migita, Seiya Harada, Yoshiki Etoh, Hideki Kobayashi, Keiko Nishi, Kaori Iwabuchi, Yukiko Hara-Kudo, Tsutomu Kubota, Ichiro Furukawa, Takayuki Kurazono, Kiyoshi Tominaga, Takashi Hirochi, Jun Terajima, Kanda Takashi, Hidemasa Izumiya, Hideki Naitoh, Tomomi Kono, Yumiko Sakamoto, Yuko Matsumoto, Norio Asai, Yukiko Asano, Makoto Ohnishi, Toshiro Kuroki, Koji Kikuchi, Sachiko Ichihara, Kazuko Seto, Hitomi Kasahara, Keiko Tanaka, Keiko Kimata, Jun Yatsuyanagi, Kunihiko Nakane, Reiko Arai, Junko Isobe, Noriko Konishi, Kimiko Kawano, Tomoko Kitahashi, Mikiko Honda, Madoka Hamada, Sunao Iyoda, Mitsuteru Takamura, Masakado Matsumoto, Takehito Saitoh, Yutaka Shiraki, Kazuki Chiba, Yoshitoshi Ogura, Hiroaki Satoh, Junko Yabata, Hiromi Obata, Masao Kawase, Kazutoshi Matsumoto, Junichi Tanabe, Midori Hiroi, Jun Kudaka, Etsuko Saitoh, Kazumi Horikawa, and Akemi Kai

Subjects
Genetics, education.field_of_study, Lineage (genetic), clade, Population, Shiga toxin, Biology, Multiple Loci VNTR Analysis, stx, Subtyping, law.invention, Major Articles, Infectious Diseases, Oncology, law, hemic and lymphatic diseases, biology.protein, EHEC, HUS, Typing, O157, Clade, education, Polymerase chain reaction
Abstract

EHEC O157:H7 clade 6 strains harboring stx2a and/or stx2c and clade 8 strains harboring stx2a or stx2a/stx2c were frequently associated with childhood HUS cases in Japan. Rapid and specific detection of such lineages are required for infection control measures., Background Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Methods Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999–2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Results Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0–9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1–3 isolates but not in clades 4–8 isolates. Conclusions Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.

Published
2014

10. A Survey for Contamination of Salmonella in Poultry 'Sashimi', and Its Preventive Studies on Preparation Process for Salmonella Control

Author

Makoto Tsubakimoto, Yoshiko Kurihara, Masaaki Naganuma, Takahiro Oda, Hiroshi Hiwaki, Mikiko Honda, and Yasutoshi Sato

Subjects
Salmonella, medicine, Environmental science, Food science, Contamination, medicine.disease_cause
Abstract

1. 生食用鶏肉類のサルモネラ汚染率は12.3%であり, 一般の加熱用鶏肉と比べると低い汚染率であったが, 生で喫食される食品であるため, 食中毒につながる危険性は大きく, 本食品の調理と保管には充分な注意が必要と考えられた.2. 生食用鶏肉類調理施設でのアンケート調査では, 作業実態が把握できたが, 各施設で調理された製品のサルモネラおよびカンピロバクター汚染の原因は明確にできなかった.3. 生食用鶏肉の調理は, [解体]―[皮剥ぎ]―[スライス]の3工程において, それぞれ専用のまな板・包丁・布巾を使用し, 各工程間には手洗いを行い, 手洗い後は使い捨て紙タオルで手指を拭く手順で行えば, 最終製品のサルモネラ汚染を最小限に抑えることが可能であることがわかった.

Published
1995
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11. Identification of Escherichia albertii as a Causative Agent of a Food-Borne Outbreak Occurred in 2003

Author

Masanori Matsuda, Kikuyo Ogata, Hiroshi Hiwaki, Hidehiro Yoshida, Mikiko Honda, Takahiro Oda, Kumiko Shigemura, and Nanami Asoshima

Subjects
DNA, Bacterial, Electrophoresis, Agar Gel, Escherichia, Microbiology (medical), Enterobacteriaceae Infections, General Medicine, Biology, biology.organism_classification, Polymerase Chain Reaction, Agar gel, Virology, Bacterial Typing Techniques, Disease Outbreaks, Escherichia albertii, Microbiology, Foodborne Diseases, Infectious Diseases, Humans, Identification (biology), Food borne outbreak
Published
2014
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12. Optical resolution of dihydropyridine enantiomers by high-performance liquid chromatography using phenylcarbamates of polysaccharides as a chiral stationary phase

Author

Masahiro Nakano, Koichi Hatada, Ryo Aburatani, Mikiko Honda, Yoshio Okamoto, and Nobuo Inotsume

Subjects
Chromatography, Chemistry, Hydrophilic interaction chromatography, Organic Chemistry, General Medicine, Reversed-phase chromatography, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, Chiral column chromatography, Column chromatography, Phenylcarbamates, Supercritical fluid chromatography, Chiral derivatizing agent
Published
1990
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13. The genome sequence and structure of rice chromosome 1

Author

Jianyu Song, Takanori Shimokawa, Hideki Nagasaki, Yuichi Ito, Kumiko Sakai, Kozue Kamiya, Takuji Sasaki, Yoshihito Niimura, Shoji Yoshiki, Mikiko Honda, Harumi Yamagata, Yoshiaki Nagamura, Satomi Hosokawa, Hiroyuki Kanamori, Hidetaka Ito, Kumiko Tsuji, Hiroshi Mizuno, Sachie Ito, Hisakazu Iwama, Tsuyu Ando, Nozomi Ono, Michie Shibata, Jiming Jiang, Noriko Kobayashi, Masahiro Yano, Huisun Zhong, Yoshiyuki Mukai, Tomoya Baba, Yoko Ichikawa, Kimihiro Terasawa, Tatsumi Mizubayashi, Ari Kikuta, Kimiko Yamamoto, Nobukazu Namiki, Yumi Nakamichi, Kayo Machita, Kazunori Waki, Manami Negishi, Ho-Il Kim, Marina Nakashima, Takashi Matsumoto, Masumi Iijima, Yuichi Katayose, Baltazar A. Antonio, Satoshi Katagiri, Zhukuan Cheng, Yukiyo Ito, Kazuko Yukawa, Chizuko Harada, Hiroko Yamane, Katsumi Sakata, Ayahiko Shomura, Takashi Gojobori, Yuko Nakama, Koji Arikawa, Aki Iwabuchi, Masao Hamada, Hiroyoshi Aoki, Mika Hayashi, Isamu Ohta, Tomoko Maehara, Yuka Takazaki, Izumi Kono, Masako Okamoto, Michiko Ikeda, Saori Hijishita, Atsuko Idonuma, Toshinori Endo, Rie Yoshihara, Kohei Arita, Masatoshi Masukawa, Shoko Saji, Jianzhong Wu, Moo-Young Eun, Yoshino Chiden, Jang Ho Hahn, Mari Nakamura, Maiko Ikeno, Tomoko Ito, and Wataru Karasawa

Subjects
Chloroplasts, Sequence analysis, Molecular Sequence Data, Arabidopsis, Sequence Homology, Locus (genetics), Biology, Genes, Plant, Genome, Sensitivity and Specificity, Chromosomes, Plant, Complete sequence, Contig Mapping, Chromosome 19, Genome size, In Situ Hybridization, Fluorescence, Synteny, Plant Proteins, Genetics, Expressed Sequence Tags, Base Composition, Multidisciplinary, food and beverages, Computational Biology, Oryza, Genome project, Exons, Sequence Analysis, DNA, Physical Chromosome Mapping, Introns, Tandem Repeat Sequences, Multigene Family, DNA Transposable Elements, Genome, Plant
Abstract

The rice species Oryza sativa is considered to be a model plant because of its small genome size, extensive genetic map, relative ease of transformation and synteny with other cereal crops. Here we report the essentially complete sequence of chromosome 1, the longest chromosome in the rice genome. We summarize characteristics of the chromosome structure and the biological insight gained from the sequence. The analysis of 43.3 megabases (Mb) of non-overlapping sequence reveals 6,756 protein coding genes, of which 3,161 show homology to proteins of Arabidopsis thaliana, another model plant. About 30% (2,073) of the genes have been functionally categorized. Rice chromosome 1 is (G + C)-rich, especially in its coding regions, and is characterized by several gene families that are dispersed or arranged in tandem repeats. Comparison with a draft sequence indicates the importance of a high-quality finished sequence.

Published
2002

14. Stereoselective analysis of the enantiomers of ethotoin in human serum using chiral stationary phase liquid chromatography and gas chromatography—mass spectrometry

Author

Masahiro Nakano, Mikiko Honda, Junko Fujii, Ichiro Matsuda, Nobuo Inotsume, and Akimasa Higashi

Subjects
Chromatography, Adolescent, Chemistry, Hydantoins, Stereoisomerism, General Chemistry, Reference Standards, Mass spectrometry, High-performance liquid chromatography, Gas Chromatography-Mass Spectrometry, Chiral column chromatography, Ethotoin, medicine, Humans, Female, Stereoselectivity, Gas chromatography, Enantiomer, Gas chromatography–mass spectrometry, Chromatography, Liquid, medicine.drug
Published
1988
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