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Istraživanja su provedena tijekom 2012. i 2013. godine na dva tipa tla: humoglej i močvarno glejno djelomično hidromeliorirano tlo. Korišten hibrid u sjetvi bila je Colonia KWS (KWS). U pokusu je korišten mikrobiološki pripravak Mikro – Vital koji u svom sastavu sadrži bakterijske vrste Azotobacter vinelandii, Azospirillum lipoferum, A. brasilense, Bacillus megaterium, B. subtilis, B. cirkulans, B. polymiksa i Pseudomonas fluorescens. Dvofaktorijalni pokus postavljen je po randomiziranom blok rasporedu u 4 ponavljanja i 6 varijanti: A1 – kontrola ; A2 – primjena mikrobiološkog pripravka Mikro – Vital (1 l ha-1) ; B1 – gnojidba dušikom na osnovi analiza tla ; B2 – reducirana gnojidba dušikom za 20% ; B3 – reducirana gnojidba dušikom za 40%. Najbolji rezultati dobiveni su uz primjenu mikrobiološkog pripravka Mikro – Vital, dok je gnojidba dušikom ovisila o kemijskim svojstvima tla, kao i o vremenskim prilikama tijekom vegetacije.// Investigations were carried out in 2012 and 2013 on two soil types Humogley and Swampy gleys partially hydroameliorated soil. Hybrid Colonia KWS (KWS) was used in sowing .In experiment we used microbial preparation Mikro – Vital which in its composition contains Azotobacter vinelandii, Azospirillum lipoferum, A. brasilense, Bacillus megaterium, B. subtilis, B. cirkulans, B. polymyxa and P. fluorescens. The experiment was set up in completely randomized block design, with 6 different variants and 4 repetitions: A1 – control ; A2 – application of microbiological preparation Mikro – Vital (1 lha-1) ; B1 – mineral nitrogen fertilization on the basis of analysis of the soil ; B2 – reduced nitrogen mineral fertilization by 20% ; B3 – reduced nitrogen mineral fertilization by 40%. The best results were obtained with the application of microbiological preparation Mikro - Vital, while nitrogen fertilization depended on the chemical properties of soil and weather conditions during the growing season.