8 results on '"Milieu INRA96"'
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2. Mise au point d'un milieu pour la cryoconservation de la semence équine et mécanismes de cryoprotection impliqués
- Author
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Pillet, Elodie, Batellier, Florence, Magistrini, Michèle, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV] Life Sciences [q-bio] ,MILIEU DE CONGELATION ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,MILIEU INRA96 ,[INFO] Computer Science [cs] - Abstract
Actualités en élevage équin; National audience
- Published
- 2010
3. High fertility rates with stallion sperm cryopreserved in INRA96® based extender are not predicted by in vitro parameters
- Author
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S. Desherces, V. Furstoss, Eric Schmitt, Dominique Kerboeuf, Elodie Pillet, G. Duchamp, M. Magistrini, Florence Batellier, Y. Le Vern, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), Infectiologie Animale et Santé Publique (UR IASP), IMV Technologies, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
040301 veterinary sciences ,Extender ,0402 animal and dairy science ,High fertility ,04 agricultural and veterinary sciences ,General Medicine ,Biology ,040201 dairy & animal science ,Sperm ,In vitro ,Cryopreservation ,law.invention ,0403 veterinary science ,Andrology ,Endocrinology ,Food Animals ,law ,[SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,Animal Science and Zoology ,MILIEU INRA96 ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2008
4. Du nouveau pour la congélation de la semence d'étalon
- Author
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Pillet, Elodie, Batellier, Florence, Magistrini, Michèle, Duchamp, Guy, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,MILIEU INRA96 ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2008
5. Le milieu INRA96® supplémenté de jaune d'oeuf et de glycérol améliore la fertilité en insémination artificielle de semence congelée
- Author
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Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Furstoss, Vincent, Bruneau, Bernard, Yvon, Jean-Marie, Le Vern, Yves, Kerboeuf, Dominique, Vidament, Marianne, Magistrini, Michèle, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), Infectiologie Animale et Santé Publique (UR IASP), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV] Life Sciences [q-bio] ,CONGELATION ,FERTILITE ,[SDV]Life Sciences [q-bio] ,ETALON ,[INFO]Computer Science [cs] ,MILIEU INRA96 ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2008
6. Freezing stallion semen in INRA96®-based extender improves fertility rates in comparison with INRA82
- Author
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Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Furstoss, Vincent, Le Vern, Yves, Kerboeuf, Dominique, Vidament, Marianne, Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), IMV Technologies, Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), and Infectiologie Animale et Santé Publique (UR IASP)
- Subjects
Cryoconservation ,[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,Stallion ,Fertility ,Semen ,Etalon ,Milieu INRA96 - Abstract
International audience; The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96 (R) extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96 (R) is a chemically- defined extender developed for fresh semen storage at 4 degrees C or 15 degrees C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96 (R) extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96 (R) than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96 (R). Further research is needed to understand how INRA96 (R) components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.
- Published
- 2008
- Full Text
- View/download PDF
7. La congélation de semence d'étalon dans un milieu à base d'INRA96® améliore le taux de fertilité en comparaison avec le milieu INRA82
- Author
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V. Furstoss, Florence Batellier, Elodie Pillet, Yves Le Vern, Dominique Kerboeuf, Guy Duchamp, Michèle Magistrini, Marianne Vidament, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), IMV Technologies, Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), and Infectiologie Animale et Santé Publique (UR IASP)
- Subjects
Cryoconservation ,[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,food.ingredient ,Etalon ,Total fertility rate ,media_common.quotation_subject ,reproduction animale ,Semen ,Fertility ,Milieu INRA96 ,Biochemistry ,Cryopreservation ,law.invention ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,food ,milieu de conservation ,law ,Yolk ,Glycerol ,conservation du sperme ,media_common ,Stallion ,030219 obstetrics & reproductive medicine ,Chemistry ,Extender ,0402 animal and dairy science ,fertilité animale ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,Sperm ,Agricultural sciences ,cheval ,semence ,Sciences agricoles ,Food Science ,équin - Abstract
International audience; The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96 (R) extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96 (R) is a chemically- defined extender developed for fresh semen storage at 4 degrees C or 15 degrees C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96 (R) extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96 (R) than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96 (R). Further research is needed to understand how INRA96 (R) components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.
- Published
- 2008
- Full Text
- View/download PDF
8. Inra96® : un milieu efficace pour congeler la semence d'étalon
- Author
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Batellier, Florence, Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,MILIEU INRA96 ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] - Abstract
"en direct des labos", la lettre électronique de l'Inra pour les entreprises; National audience
- Published
- 2008
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