Search

Additional file 6: Figure S6. The overexpression of TaKNOX11-A enhanced drought and salt tolerance in Arabidopsis. a) Root length assays of wild-type (WT), TaKNOX11-A transgenic Arabidopsis and mutant Arabidopsis (knat3) seeds treated with 10% PEG6000 and 100 mM NaCl. b) Total root lengths of seedlings. c) Fresh weight of normal and stressed Arabidopsis. Each strains used three samples in different treatments for further statistical analysis. Three biological replicates were done for each treatment. The error bars indicate the SD of the three replicates. Asterisks indicate significant differences between WT, OE, and mutant lines (*p

Additional file 5: Figure S5. Germination test of wild-type (WT), TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) seeds under NaCl treatment. a) Phenotypes of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) seeds treated with 0.5 �� MS, and 100 mM and 150 mM NaCl. b) Germination rate of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) seeds treated with MS, and 100 mM and 150 Mm NaCl. Each strain used 36 samples in different treatments for further statistical analysis. Three biological replicates were done for each treatment. The germination rate was counted every 12 h. The error bars indicate the SD of the three replicates.

Additional file 2: Figure S2. Protein���protein interaction network of wheat TALE proteins.

Additional file 8: Figure S8. The overexpression of TaKNOX11-A enhanced salt tolerance in Arabidopsis. Mutant Arabidopsis (knat3) showed lower salt resistance compared to wild-type (WT) plants. a) NaCl tolerance phenotypes of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) in soil. Three-week-old seedlings of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) lines were salt stressed for 1 week and then rewatered for 3 days. b) Survival rate of normal and salt-stressed Arabidopsis. c-d) Proline and malondialdehyde content were detected in WT, OE, and mutant plants under normal growth and salt stress conditions. Twelve samples per strains were used for the salt stress treatment and for further statistical analysis. Three biological replicates were done for each treatment. Data are presented as the mean �� SD of three independent replicates. Asterisks indicate significant differences between WT, OE, and mutant lines (*p

Additional file 1: Figure S1. Syntenic and Evolutionary analyses in wheat TALE family. a) UpSet plot of non-redundant TALE genes in different species. b) Violin plot of Ka/Ks rations in duplicated TALE gene pairs.

Additional file 3: Figure S3. GO annotation of TALE genes in wheat. Biological processes (a), molecular functions (b), and cellular components (c) are annotated.

Additional file 4: Figure S4. Germination test of wild-type (WT), TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) seeds under PEG6000 treatment. a) Phenotypes of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) seeds treated with 0.5 �� MS, 8 and 10% PEG6000. b) Germination rates of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) seeds treated with MS, 8 and 10% PEG6000. Each strain used 36 samples in different treatments for further statistical analysis. Three biological replicates were done for each treatment. The germination rate was counted every 12 h. The error bars indicate the SD of the three replicates.

Additional file 7: Figure S7. The overexpression of TaKNOX11-A enhanced drought tolerance in Arabidopsis. a) Drought tolerance phenotypes of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) in soil. Three-week-old seedlings of WT, TaKNOX11-A transgenic Arabidopsis, and mutant Arabidopsis (knat3) lines were dehydrated for 1 week and then rehydrated for 3 days. b) Survival rate of normal and drought-stressed Arabidopsis. c-d) Proline and malondialdehyde content were detected in WT, OE, and mutant plants under normal growth and drought conditions. Twelve samples per strains were used for the drought treatment and for further statistical analysis. Three biological replicates were done for each treatment. Data were presented as the mean �� SD of three independent replicates. Asterisks indicate significant differences between WT, OE, and mutant lines (*p

Additional file 7: Figure S7. The overexpression of TaSCPL184-6D enhanced drought tolerance in Arabidopsis. a The drought tolerance phenotypes of WT and TaSCPL184-6D transgenic Arabidopsis in soil. Three-week-old seedlings of WT and TaSCPL184-6D-overexpressing lines were dehydrated for 2 weeks and then rewatered for 3 days. b Statistical analysis of survival rates. c The amount of Pro. d The amount of MDA. Data is shown as the mean ± SD of three independent replicates. Significant differences were observed using a Student’ s t test (*p

Additional file 3: Figure S3. The number of SSRs per chromosome.

Additional file 8: Figure S8. The overexpression of TaSCPL184-6D enhanced the tolerance to NaCl tolerance in Arabidopsis. a NaCl tolerance phenotypes of WT and TaSCPL184-6D transgenic Arabidopsis in soil. Three-week-old seedlings of WT and TaSCPL184-6D-overexpressing lines were stressed for 7 days and then rewatered for 3 days. b Statistical analysis of survival rates. c The amount of Pro. d The amount of MDA.

Additional file 4: Figure S4. The overexpression of TaSCPL184-6D increased the germination rate of seeds under PEG6000 treatment. a The phenotypes of WT and TaSCPL184-6D transgenic Arabidopsis seeds under 8 and 10% PEG6000 treatments. b The germination rates of WT and TaSCPL184-6D transgenic Arabidopsis seeds at different time points on MS medium. c The germination rates under 8% PEG6000 treatment. d The germination rates under 10% PEG6000 treatment. A total of three biological replicates were performed. The error bars indicate the standard deviation (SD) of the three replicates.

Additional file 5: Figure S5. The overexpression of TaSCPL184-6D increased the germination rate of seeds under NaCl treatment. a The phenotypes of WT and TaSCPL184-6D transgenic Arabidopsis seeds under 100 mM and 150 mM NaCl treatments. b The germination rates of WT and TaSCPL184-6D transgenic Arabidopsis seeds at different time points on MS medium. c The germination rates under 150 mM NaCl treatment. d The germination rates under 10% PEG6000 treatment. A total of three biological replicates were performed. The error bars indicate the SD of the three replicates.

Additional file 2: Figure S2. Phylogenetic relationship, gene structure and conserved motifs analysis of 209 TaSCPL genes.

Additional file 1: Figure S1. The research process of this study.