Search

Your search keyword '"Min Woo Sung"' showing total 1,076 results
Start Over Author "Min Woo Sung"
1,076 results on '"Min Woo Sung"'

1. Full-length human cytomegalovirus terminase pUL89 adopts a two-domain structure specific for DNA packaging.

Author

Janine Theiß, Min Woo Sung, Andreas Holzenburg, and Elke Bogner

Subjects
Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5
Abstract

A key step in replication of human cytomegalovirus (HCMV) in the host cell is the generation and packaging of unit-length genomes into preformed capsids. The enzymes involved in this process are the terminases. The HCMV terminase complex consists of two terminase subunits, the ATPase pUL56 and the nuclease pUL89. A potential third component pUL51 has been proposed. Even though the terminase subunit pUL89 has been shown to be essential for DNA packaging and interaction with pUL56, it is not known how pUL89 mechanistically achieves sequence-specific DNA binding and nicking. To identify essential domains and invariant amino acids vis-a-vis nuclease activity and DNA binding, alanine substitutions of predicted motifs were analyzed. The analyses indicated that aspartate 463 is an invariant amino acid for the nuclease activity, while argine 544 is an invariant aa for DNA binding. Structural analysis of recombinant protein using electron microscopy in conjunction with single particle analysis revealed a curvilinear monomer with two distinct domains connected by a thinner hinge-like region that agrees well with the predicted structure. These results allow us to model how the terminase subunit pUL89's structure may mediate its function.

Published
2019
Full Text
View/download PDF

2. Mechanism of pharmacochaperoning in a mammalian KATP channel revealed by cryo-EM

Author

Gregory M Martin, Min Woo Sung, Zhongying Yang, Laura M Innes, Balamurugan Kandasamy, Larry L David, Craig Yoshioka, and Show-Ling Shyng

Subjects
ABC transporter, glibenclamide, repaglinide, carbamazepine, sulfonylurea receptor, inward rectifier potassium channel, Medicine, Science, Biology (General), QH301-705.5
Abstract

ATP-sensitive potassium (KATP) channels composed of a pore-forming Kir6.2 potassium channel and a regulatory ABC transporter sulfonylurea receptor 1 (SUR1) regulate insulin secretion in pancreatic β-cells to maintain glucose homeostasis. Mutations that impair channel folding or assembly prevent cell surface expression and cause congenital hyperinsulinism. Structurally diverse KATP inhibitors are known to act as pharmacochaperones to correct mutant channel expression, but the mechanism is unknown. Here, we compare cryoEM structures of a mammalian KATP channel bound to pharmacochaperones glibenclamide, repaglinide, and carbamazepine. We found all three drugs bind within a common pocket in SUR1. Further, we found the N-terminus of Kir6.2 inserted within the central cavity of the SUR1 ABC core, adjacent the drug binding pocket. The findings reveal a common mechanism by which diverse compounds stabilize the Kir6.2 N-terminus within SUR1’s ABC core, allowing it to act as a firm ‘handle’ for the assembly of metastable mutant SUR1-Kir6.2 complexes.

Published
2019
Full Text
View/download PDF

3. RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana

Author

Zhonghui Zhang, Fuqu Hu, Min Woo Sung, Chang Shu, Claudia Castillo-González, Hisashi Koiwa, Guiliang Tang, Martin Dickman, Pingwei Li, and Xiuren Zhang

Subjects
argonaute, RICE, exoribonuclease, miRNAs, RISC, uridylation, Medicine, Science, Biology (General), QH301-705.5
Abstract

RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation. Here, we identified novel cofactors of Arabidopsis AGOs, named RICE1 and RICE2. RICE proteins specifically degraded single-strand (ss) RNAs in vitro; but neither miRNAs nor miRNA*s in vivo. RICE1 exhibited a DnaQ-like exonuclease fold and formed a homohexamer with the active sites located at the interfaces between RICE1 subunits. Notably, ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels; but also increased 5' cleavage RISC fragments with extended uridine tails. We conclude that RICEs act to degrade uridylated 5’ products of AGO cleavage to maintain functional RISC. Our study also suggests a possible link between decay of cleaved target mRNAs and miRNA stability in RISC.

Published
2017
Full Text
View/download PDF

5. Role of CXCR4 Antagonist in Megakaryocyte Reinstatement with Increased Sinusoidal Vessel Density

Author

Lee, Ji Yoon, Han, A-Reum, Hwang, Hee-sun, Kim, Donghyun Curt, Min, Woo-Sung, Kim, Hee-Je, COHEN, IRUN R., Editorial Board Member, LAJTHA, ABEL, Editorial Board Member, LAMBRIS, JOHN D., Editorial Board Member, PAOLETTI, RODOLFO, Editorial Board Member, REZAEI, NIMA, Editorial Board Member, Thews, Oliver, editor, LaManna, Joseph C., editor, and Harrison, David K., editor

Published
2018
Full Text
View/download PDF

12. Beneficial Role of Low-Dose Antithymocyte Globulin in Unrelated Stem Cell Transplantation for Adult Patients with Acquired Severe Aplastic Anemia: Reduction of Graft-versus-Host Disease and Improvement of Graft-versus-Host Disease–Free, Failure-Free Survival Rate

Author

Park, Sung-Soo, Kwak, Dae Hun, Jeon, Young-Woo, Yoon, Jae-Ho, Lee, Sung-Eun, Cho, Byung-Sik, Eom, Ki-Seong, Kim, Yoo-Jin, Kim, Hee-Je, Lee, Seok, Min, Chang-Ki, Cho, Seok-Goo, Kim, Dong-Wook, Min, Woo-Sung, and Lee, Jong Wook

Published
2017
Full Text
View/download PDF

16. Negative Impact of Unidirectional Host-versus-Graft Killer Cell Immunoglobulin–like Receptor Ligand Mismatch on Transplantation Outcomes after Unmanipulated Haploidentical Peripheral Blood Stem Cell Transplantation for Acute Myeloid Leukemia

Author

Yahng, Seung-Ah, Jeon, Young-Woo, Yoon, Jae-Ho, Shin, Seung-Hwan, Lee, Sung-Eun, Cho, Byung-Sik, Eom, Ki-Seong, Kim, Yoo-Jin, Lee, Seok, Min, Chang-Ki, Cho, Seok-Goo, Kim, Dong-Wook, Lee, Jong-Wook, Min, Woo-Sung, and Kim, Hee-Je

Published
2016
Full Text
View/download PDF

19. Wilms Tumor Gene 1 Expression as a Predictive Marker for Relapse and Survival after Hematopoietic Stem Cell Transplantation for Myelodysplastic Syndromes

Author

Yoon, Jae-Ho, Jeon, Young-Woo, Yahng, Seung-ah, Shin, Seung-Hwan, Lee, Sung-Eun, Cho, Byung-Sik, Lee, Dong-Gun, Eom, Ki-Seong, Kim, Hee-Je, Lee, Seok, Min, Chang-Ki, Cho, Seok-Goo, Kim, Yonggoo, Kim, Dong-Wook, Lee, Jong-Wook, Han, Kyungja, Min, Woo-Sung, Park, Chong-Won, Kim, Myungshin, and Kim, Yoo-Jin

Published
2015
Full Text
View/download PDF

21. A Well-Tolerated Regimen of 800 cGy TBI-Fludarabine-Busulfan-ATG for Reliable Engraftment after Unmanipulated Haploidentical Peripheral Blood Stem Cell Transplantation in Adult Patients with Acute Myeloid Leukemia

Author

Yahng, Seung-Ah, Kim, Jung-Ho, Jeon, Young-Woo, Yoon, Jae-Ho, Shin, Seung-Hwan, Lee, Sung-Eun, Cho, Byung-Sik, Eom, Ki-Seong, Kim, Yoo-Jin, Lee, Seok, Min, Chang-Ki, Cho, Seok-Goo, Kim, Dong-Wook, Lee, Jong-Wook, Min, Woo-Sung, Park, Chong-Won, and Kim, Hee-Je

Published
2015
Full Text
View/download PDF

27. Ligand-mediated Structural Dynamics of a Mammalian Pancreatic K

Author

Min Woo, Sung, Camden M, Driggers, Barmak, Mostofian, John D, Russo, Bruce L, Patton, Daniel M, Zuckerman, and Show-Ling, Shyng

Subjects
Adenosine Triphosphate, KATP Channels, Protein Conformation, Humans, Ligands, Pancreas
Abstract

Regulation of pancreatic K

Published
2022

28. Cytogenetic profiles of 2806 patients with acute myeloid leukemia—a retrospective multicenter nationwide study

Author

Byun, Ja Min, Kim, Young Jin, Yoon, Hwi-Joong, Kim, Si-Young, Kim, Hee-Je, Yoon, Jaeho, Min, Yoo Hong, Cheong, Jun-Won, Park, Jinny, Lee, Jae Hoon, Hong, Dae Sik, Park, Seong Kyu, Kim, Hyeoung-Joon, Ahn, Jae-Sook, Shin, Ho-Jin, Chung, Joo Seop, Lee, Won Sik, Lee, Sang Min, Park, Yong, Kim, Byung Soo, Lee, Je-Hwan, Lee, Kyoo-Hyung, Jung, Chul Won, Jang, Jun Ho, Min, Woo-Sung, Park, Tae Sung, and on behalf of the AML/MDS working party of Korean Society of Hematology

Published
2016
Full Text
View/download PDF

30. Ligand-mediated structural dynamics of a mammalian pancreatic KATP channel

Author

Min Woo Sung, Camden M. Driggers, Barmak Mostofian, John D. Russo, Bruce L. Patton, Daniel M. Zuckerman, and Show-Ling Shyng

Subjects
endocrine system
Abstract

Regulation of pancreatic KATP channels involves orchestrated interactions of channel subunits, Kir6.2 and SUR1, and their ligands. How ligand interactions affect channel conformations and activity is not well understood. To elucidate the structural correlates pertinent to ligand interactions and channel gating, we compared cryo-EM structures of channels in the presence and absence of pharmacological inhibitors and ATP, focusing on channel conformational dynamics. We found pharmacological inhibitors and ATP enrich a channel conformation in which the Kir6.2 cytoplasmic domain is closely associated with the transmembrane domain relative to one where the Kir6.2 cytoplasmic domain is extended away into the cytoplasm. This conformation change remodels a network of intra and inter-subunit interactions as well as both the ATP and PIP2 binding pockets. The structures resolved key contacts between the distal N-terminus of Kir6.2 and SUR1’s ABC module involving residues implicated in channel function. A SUR1 residue, K134, is identified to directly contribute to the PIP2 binding pocket. Molecular dynamics simulations revealed two Kir6.2 residues, K39 and R54, that mediate both ATP and PIP2 binding, suggesting a mechanism for competitive gating by ATP and PIP2.

Published
2022

36. Vascular K ATP channel structural dynamics reveal regulatory mechanism by Mg-nucleotides

Author

Zhongying Yang, Bruce L. Patton, Min Woo Sung, Camden M. Driggers, Daniel M. Zuckerman, John D. Russo, Show-Ling Shyng, and Barmak Mostofian

Subjects
Gene isoform, chemistry.chemical_classification, endocrine system, Multidisciplinary, ATP-sensitive potassium channel, Chemistry, Glutamate receptor, ATP-binding cassette transporter, Inhibitory postsynaptic potential, Glibenclamide, medicine, Biophysics, Nucleotide, Linker, hormones, hormone substitutes, and hormone antagonists, medicine.drug
Abstract

Vascular tone is dependent on smooth muscle KATP channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantu syndrome. Unique among KATP isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined cryogenic electron microscopy structures of vascular KATP channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic KATP channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational "propeller" and "quatrefoil" geometries surrounding their Kir6.1 core. The glutamate/aspartate-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. Molecular dynamics simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B, and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated, and MgADP-bound activated conformations wherein the glutamate/aspartate-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward KATP channel activation.

Published
2021

38. Vascular K

Author

Min Woo, Sung, Zhongying, Yang, Camden M, Driggers, Bruce L, Patton, Barmak, Mostofian, John D, Russo, Daniel M, Zuckerman, and Show-Ling, Shyng

Subjects
Cantú syndrome, endocrine system, Kir6.1, Potassium Channels, Hypertrichosis, Cardiomegaly, Muscle, Smooth, Biological Sciences, Osteochondrodysplasias, Sulfonylurea Receptors, Biochemistry, Adenosine Diphosphate, sulfonylurea receptor 2B, Structure-Activity Relationship, Adenosine Triphosphate, KATP Channels, ATP-sensitive potassium channel, Humans, ABC transporter, Potassium Channels, Inwardly Rectifying, Pancreas
Abstract

Significance Vascular KATP channels formed by the potassium channel Kir6.1 and its regulatory protein SUR2B maintain blood pressure in the physiological range. Overactivity of the channel due to genetic mutations in either Kir6.1 or SUR2B causes severe cardiovascular pathologies known as Cantú syndrome. The cryogenic electron microscopy structures of the vascular KATP channel reported here show multiple, dynamically related conformations of the regulatory subunit SUR2B. Molecular dynamics simulations reveal the negatively charged ED-domain in SUR2B, a stretch of 15 glutamate (E) and aspartate (D) residues not previously resolved, play a key MgADP-dependent role in mediating interactions at the interface between the SUR2B and Kir6.1 subunits. Our findings provide a mechanistic understanding of how channel activity is regulated by intracellular MgADP., Vascular tone is dependent on smooth muscle KATP channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among KATP isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined cryogenic electron microscopy structures of vascular KATP channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic KATP channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational “propeller” and “quatrefoil” geometries surrounding their Kir6.1 core. The glutamate/aspartate-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. Molecular dynamics simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B, and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated, and MgADP-bound activated conformations wherein the glutamate/aspartate-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward KATP channel activation.

Published
2021

43. High Levels of B Cell Activating Factor During the Peritransplantation Period Are Associated with a Reduced Incidence of Acute Graft-versus-Host Disease following Myeloablative Allogeneic Stem Cell Transplantation

Author

Cho, Byung-Sik, Min, Chang-Ki, Kim, Hee-Je, Lee, Seok, Kim, Yoo-Jin, Lim, Ji-Young, Jeong, Dae-Chul, Cho, Bin, Kim, Hack-Ki, Eom, Ki-Seong, Cho, Seok-Goo, Kim, Dong-Wook, Lee, Jong-Wook, Min, Woo-Sung, Kim, Chun-Choo, and Chung, Nak-Gyun

Published
2010
Full Text
View/download PDF

44. Vascular KATP channel structural dynamics reveal regulatory mechanism by Mg-nucleotides

Author

Barmak Mostofian, Show-Ling Shyng, Min Woo Sung, Zhongying Yang, Daniel M. Zuckerman, Bruce L. Patton, and John D. Russo

Subjects
Gene isoform, chemistry.chemical_classification, endocrine system, Chemistry, Mechanism (biology), Inhibitory postsynaptic potential, Glibenclamide, Smooth muscle, Katp channels, Biophysics, medicine, Nucleotide, Linker, medicine.drug
Abstract

Vascular tone is dependent on smooth muscle KATP channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among KATP isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined the first cryoEM structures of vascular KATP channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic KATP channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational “propeller” and “quatrefoil” geometries surrounding their Kir6.1 core. The previously unseen ED-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. MD simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated and MgADP-bound activated conformations wherein the ED-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward KATP channel activation.

Published
2021

47. The efficacy of rabbit antithymocyte globulin with cyclosporine in comparison to horse antithymocyte globulin as a first-line treatment in adult patients with severe aplastic anemia: a single-center retrospective study

Author

Shin, Seung-Hwan, Yoon, Jae-Ho, Yahng, Seung-Ah, Lee, Sung-Eun, Cho, Byung-Sik, Eom, Ki-Sung, Kim, Yoo-Jin, Lee, Seok, Min, Chang-Ki, Kim, Hee-Je, Cho, Seok-Goo, Kim, Dong-Wook, Min, Woo-Sung, Park, Chong-Won, and Lee, Jong Wook

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results