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We developed a SMEDDS to enhance the oral delivery of resveratrol by using high performance liquid chromatography, a pseudo ternary phase diagram and a central composite design (CCD). We found that the optimal formulation of 12.69% greoil gtcc, 62.29% Cremophor EL, and 25.02% Labrasol. We characterized the particle size and zeta potential of the final SMEDDS.

The importance of fast-closed valve for mitigating accident sequent when the pipeline of the tanks stored flammable liquids discussed in this paper broken was illuminated through studying on rules and analyzing accident. And the offshore platforms and ships were as example to list the relative codes and rules force requirements on fast-closed valve. By studying the typical process easy ignored in design - leakage due to siphoning effect, and the same time some typical pipelines and fast-closed valve arrangement and the corresponding alternative measures were given. And suggested that the storage tanks stored toxic and corrosive liquid or other dangerous liquids shall install a fast-closed valve or corresponding alternative measures in its pipeline for reducing the accident consequences due to leakage.

Plant annexins represent a multigene family involved in cellular elongation and development. A cDNA encoding a novel annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library and designated GhAnx1. This gene encodes a 316 amino acid protein with a theoretical molecular mass of 36.06 kDa and a theoretical pI of 6.19. At the amino acid level, it shares high sequence similarity and has evolutionary relationships with annexins from higher plants. The purified recombinant protein expressed in Escherichia coli was used to investigate its physicochemical properties. Circular dichroism spectrum analyses showed a positive peak rising to the maximum at 196 nm and a broad negative band rounding 215 nm, suggesting that the GhAnx1 protein was prominently α-helical. The fluorescence measurements indicated that it could bind to Ca(2+) in vitro. These results demonstrated that GhAnx1 was a typical annexin protein in cotton. A bioassay experiment was conducted to analyze its potential function and showed that E. coli cells expressing GhAnx1 were protected from tert-butyl hydroperoxide (tBH) stress, suggesting that it had a potential antioxidative role. Northern blot analyses revealed that GhAnx1 was highly expressed in fibers, especially during the elongation stage, suggesting that it might be important for fiber elongation.

SecA, an essential component of the general protein secretion pathway of bacteria, is present in Escherichia coli as soluble and membrane-integral forms. Here we show by electron microscopy that SecA assumes two characteristic forms in the presence of phospholipid monolayers: dumbbell-shaped elongated structures and ring-like pore structures. The ring-like pore structures with diameters of 8 nm and holes of 2 nm are found only in the presence of anionic phospholipids. These ring-like pore structures with larger 3- to 6-nm holes (without staining) were also observed by atomic force microscopic examination. They do not form in solution or in the presence of uncharged phosphatidylcholine. These ring-like phospholipid-induced pore-structures may form the core of bacterial protein-conducting channels through bacterial membranes.

A new approach to the colorimetric detection of human lung carcinoma cell by enlarging Au-nanoparticles in situ has been reported. The results show that the sensitivity of detecting lung carcinoma cell is ca. 100 cell mL-1. By enlarging Au-nanoparticles immobilized on the nitrocellulose strips in situ with 0.01% HAuCl4 and 0.4 mM NH2OH, detection of lung carcinoma cell by naked eye is available. It will be of great significance in rapid and early clinical diagnosis.