38 results on '"Ming-Chin Chan"'
Search Results
2. Surveillance for coronavirus diseases 2019 (COVID-19) among health care workers at a medical center in Taiwan, March to August 2020
- Author
-
Ming-Chin Chan, Tzu-Jou Cho, Feng-Yee Chang, and Jung-Chung Lin
- Subjects
Medicine (General) ,R5-920 - Published
- 2021
- Full Text
- View/download PDF
3. A retrospective study to estimate serum vancomycin trough concentrations in pediatric patients with current recommended dosing regimen
- Author
-
Chia-Ning Chang, Wen-Tsung Lo, Ming-Chin Chan, and Chih-Chien Wang
- Subjects
Methicillin-resistant Staphylococcus aureus ,pediatric dose ,trough concentration ,vancomycin ,Medicine ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Background: Vancomycin is widely prescribed to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). According to the Infectious Disease Society of America 2011 MRSA guidelines for adults, trough concentrations of 15–20 μg/ml were recommended with vancomycin 15–20 mg/kg/dose every 8–12 h not to exceed 2 g per dose in patients with normal renal function. As the data for dosage and monitoring of vancomycin in children are still controversial, physicians often follow the guidelines established for adults. To evaluate serum vancomycin trough concentrations in pediatric patients with currently recommended dosing regimen, we evaluated clinical data. Methods: This retrospective observational study collected pediatric patients aged
- Published
- 2018
- Full Text
- View/download PDF
4. Clinical Application of Ultraviolet C Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 in Contaminated Hospital Environments
- Author
-
Wen-Lin Su, Chih-Pei Lin, Hui-Ching Huang, Yao-Kuang Wu, Mei-Chen Yang, Sheg-Kang Chiu, Ming-Yieh Peng, Ming-Chin Chan, and You-Chen Chao
- Subjects
SARS-CoV-2 ,COVID-19 ,environmental sampling ,RT-PCR ,254 nm Ultraviolet C ,Microbiology ,QR1-502 - Abstract
To overcome the ongoing coronavirus disease 2019 (COVID-19) pandemic, transmission routes, such as healthcare worker infection, must be effectively prevented. Ultraviolet C (UVC) (254 nm) has recently been demonstrated to prevent environmental contamination by infected patients; however, studies on its application in contaminated hospital settings are limited. Herein, we explored the clinical application of UVC and determined its optimal dose. Environmental samples (n = 267) collected in 2021 were analyzed by a reverse transcription-polymerase chain reaction and subjected to UVC irradiation for different durations (minutes). We found that washbasins had a high contamination rate (45.5%). SARS-CoV-2 was inactivated after 15 min (estimated dose: 126 mJ/cm2) of UVC irradiation, and the contamination decreased from 41.7% before irradiation to 16.7%, 8.3%, and 0% after 5, 10, and 15 min of irradiation, respectively (p = 0.005). However, SARS-CoV-2 was still detected in washbasins after irradiation for 20 min but not after 30 min (252 mJ/cm2). Thus, 15 min of 254-nm UVC irradiation was effective in cleaning plastic, steel, and wood surfaces in the isolation ward. For silicon items, such as washbasins, 30 min was suggested; however, further studies using hospital environmental samples are needed to confirm the effective UVC inactivation of SARS-CoV-2.
- Published
- 2021
- Full Text
- View/download PDF
5. An investigation of vancomycin minimum inhibitory concentration creep among methicillin-resistant Staphylococcus aureus strains isolated from pediatric patients and healthy children in Northern Taiwan
- Author
-
Chia-Ning Chang, Wen-Tsung Lo, Ming-Chin Chan, Ching-Mei Yu, and Chih-Chien Wang
- Subjects
Methicillin-resistant Staphylococcus aureus ,Pulsed-field gel electrophoresis type of MRSA ,Vancomycin creep ,Microbiology ,QR1-502 - Abstract
Background and purpose: The phenomenon of vancomycin minimum inhibitory concentration (MIC) creep is an increasingly serious problem in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. In this study, we investigated the vancomycin and daptomycin MIC values of MRSA strains isolated from pediatric patients and MRSA colonized healthy children. Then, we assessed whether there was evidence of clonal dissemination for strains with an MIC to vancomycin of ≥ 1.5 μg/mL. Methods: We collected clinical MRSA isolates from pediatric patients and from healthy children colonized with MRSA during 2008–2012 at a tertiary medical center in northern Taiwan and obtained vancomycin and daptomycin MIC values using the Etest method. Pulse-field gel electrophoresis (PFGE) and staphylococcal cassette chromosome (SCCmec) typing were used to assess clonal dissemination for strains with an MIC to vancomycin of ≥ 1.5 μg/mL. Results: A total 195 MRSA strains were included in this study; 87 were isolated patients with a clinical MRSA infection, and the other 108 strains from nasally colonized healthy children. Vancomycin MIC≥1.5 μg/mL was seen in more clinical isolates (60/87, 69%) than colonized isolates (32/108, 29.6%), p
- Published
- 2017
- Full Text
- View/download PDF
6. Erratum to: Abstracts from the 8th International Congress of the Asia Pacific Society of Infection Control (APSIC)
- Author
-
Teresa Conceição, Hermínia de Lencastre, Marta Aires-de-Sousa, Rocio Alvarez Marin, Marta Aires de Sousa, Nicolas Kieffer, Patrice Nordmann, Laurent Poirel, Wison Laochareonsuk, Sireekul Petyu, Pawin Wanasitchaiwat, Sutasinee Thana, Chollathip Bunyaphongphan, Woranan Boonsomsuk, Pakpoom Maneepongpermpoon, Silom Jamulitrat, Terrence Rohan Chinniah, Kavitha Prabu, Rashidah Ahmad, Susylawathi Magon, Jauharatud DiniSuhaimi, Aizzuddin Mirasin, Nurul Morni, Boon Chu, Azizah Samsuddin, Aliyah Ahmad, Amalina Sidek, Noraini Ajis, Amalina AbuBakar, Amanie Shafiee, Julaini Safar, Ming-Chin Chan, Chih-Chien Wang, Nattawipa Boonkirdram, Wilawan Picheansathian, Pimpaporn Klunklin, Hang Thi Phan, Anh Pham Phuong Dinh, and Tuyet Thi Kim Nguyen
- Subjects
Infectious and parasitic diseases ,RC109-216 - Published
- 2017
- Full Text
- View/download PDF
7. Abstracts from the 8th International Congress of the Asia Pacific Society of Infection Control (APSIC)
- Author
-
Nantanit Sutthiruk, Mari Botti, Julie Considine, Andrea Driscoll, Ana Hutchinson, Kumthorn Malathum, Cucunawangsih Cucunawangsih, Veronica Wiwing, Vivien Puspitasari, Rathina Kumar Shanmugakani, Yukihiro Akeda, Takuya Kodera, Pitak Santanirand, Kazunori Tomono, Takayuki Yamanaka, Hiroyuki Moriuchi, Hiroyuki Kitajima, Yuho Horikoshi, Alyona Lavrinenko, Ilya Azizov, Nurlan Tabriz, Margulan Kozhamuratov, Yekatherine Serbo, Dahae Yang, Woonhyoung Lee, Il Kwon Bae, Jae Hyun Lee, Hyukmin Lee, Jung Ok Kim, Seok Hoon Jeong, Kyungwon Lee, Thiba Peremalo, Priya Madhavan, Sharina Hamzah, Leslie Than, Eng Hwa Wong, Mohd Nasir Mohd Desa, Kee Peng Ng, Marionne Geronimo, Maria Fe Tayzon, Maria Jesusa Maño, Angela Chow, Pei-Yun Hon, Mar-Kyaw Win, Brenda Ang, Yee-Sin Leo, Tina See, Rocio Alvarez Marin, Marta Aires de Sousa, Nicolas Kieffer, Patrice Nordmann, Laurent Poirel, Wison Laochareonsuk, Sireekul Petyu, Pawin Wanasitchaiwat, Sutasinee Thana, Chollathip Bunyaphongphan, Woranan Boonsomsuk, Pakpoom Maneepongpermpoon, Silom Jamulitrat, Dorairajan Sureshkumar, Kalyanaraman Supraja, Soundararajan Sharmila, Benny Setiawan, Nicolaski Lumbuun, Haruo Nakayama, Toshiko Ota, Naoko Shirane, Chikako Matuoka, Kentaro Kodama, Masanobu Ohtsuka, Silverose Ann Andales Bacolcol, Melecia Velmonte, Allan Alde, Keithleen Chavez, Arlene Joy Esteban, Aisa Jensen Lee, Tai-Chin Hsieh, Shio-ShinJean, Huey-Jen Huang, Shu-Ju Huang, Yu-Huan Huang, Pei-Chen Cheng, Su-Fang Yu, Shih-Ming Tsao, Yuan-Ti Lee, Chien-Feng Li, Min-Chi Lu, Nattapol Pruetpongpun, Thana Khawcharoenporn, Pansachee Damronglerd, Nuntra Suwantarat, Anucha Apisarnthanarak, Sasinuch Rutjanawech, Lisa Cushinotto, Patty McBride, Harding Williams, Hans Liu, Phan Thi Hang, Dinh Pham Phuong Anh, Ngai Le, Dung Khu, Lam Nguyen, Roel Beltran Castillo, Ram Gopalakrishnan, Venkatasubramanian Ramasubramanian, Subramanian Sreevidya, Ranganathan Jayapradha, Atsushi Umetsu, Tetsuhiro Noda, Kenyuu Hashimoto, Akihiro Hayashi, Mikie Kabashima, Ursula Jadczak, Knut Elvelund, Marit Johnsen, Bente Borgen, Egil Lingaas, Chia-Hua Mao, Fu-Chieh Chang, Chang-Pan Liu, Ru-Hui Chao, Fu-chieh Chang, Chang-pan Liu, Junpen Pawapotako, Chadanan Prasertpan, Wantanee Malaihuan, Phisit Uirungroj, Chalermpong Saenjum, Teerapat Ouirungrog, Sue Borrell, Pauline Bass, Leon Worth, Zhao Xian-li, Li Xiao-long, Yao Xue-hua, Ren Wei, Zhang Xia Zeng, Man Ying Kong, Christopher Koon Chi Lai, Suet Yi Lee, Ngai Chong Tsang, M. M. O’Donoghue, M. V. Boost, L. K. P. Suen, G. K. Siu, K. W. Mui, C. K. C. Lai, D. N. C. Tsang, Yuka Sato, Mariko Tateishi, Mutsuko Mihashi, Jose Paulo Flor, Marko Bautista, V. Jay De Roxas, Justine Vergara, Nicolo Andrei Añonuevo, Marion Kwek, Jose Acuin, Anna Josea Sanchez, Avel Bathan, Jamilah Binte Jantan, Chua Chor Guek, Eu Chiow Kian, Pampe Anak Pirido, Nur Fadilah Binte Mohd Aron, Leah May Estacio, Francis Alvarez Palana, Michelle Gracia, Nur Syafiqah Binte Shamsuddin, Kersten Timbad Castro, Madonna Baloria, Faezah Binte Adam, Zhang Wei, Poh Bee Fong, Marimuthu Kalisvar, I-Ju Chuang, Yi-ChunCho, Yu-Fen Chiu, Lung-Chih Chen, Yi-Chun Lin, Shao-Xing Dong, Yi-Chieh Lee, Hui-Chen Kuan, Hsin-Hua Lin, Chia-Chun Chi, Chin-Te Lu, Tang Ya-Fen, Su Li-Hsiang, Liu Jien-Wei, Hsuehlan Chao, PinRu ChangChien, WeiFang Chen, ChungHsu Lai, Lutfe Ara, Syed Mohammad Niaz Mowla, Shaikh Mahmud Kamal Vashkar, Wai Fong Chan, Mabel Yin ChunYau, Karen Kam LingChong, Tze OnLi, Rajwinder Kaur, Ng Po Yan, Gloria Chor Shan Chiu, Christina W. Y. Cheung, Patricia T. Y. Ching, Radley H. C. Ching, Conita H. S. Lam, C. H. Kan, Shirley S. Y. Lee, C. P. Chen, Regina F. Y. Chan, Annie F. Y. Leung, Isadora L. C. Wong, S. S. Lam, Queenie W. L. Chan, Cecilia Chan, Seyed Sadeq Seyed Nematian, Charles John Palenik, Mehrdad Askarian, Nahid Hatam, Itaru Nakamura, Hiroaki Fujita, Ayaka Tsukimori, Takehito Kobayashi, Akihiro Sato, Shinji Fukushima, Tetsuya Matsumoto, V. James De Roxas, V James De Roxas, Nicolo AndreiAñonuevo, Yeng May Ho, Jia Qi Kum, Bee Fong Poh, Kalisvar Marimuthu, Tzu-Yin Liu, Sin-Man Chu, Hui-Zhu Chen, Tun-chieh Chen, Yichun Chen, Ya-Ching Tsao, Sumawadee Skuntaniyom, Pirawadee Tipluy, Sangwan Paengta, Ratchanee wongsaen, Sutthiphun thanomphan, Samettanet Tariyo, Buachan Thongchuea, Pattama Khamfu, Sutthiphan Thanomphan, Wipaporn Natalie Songtaweesin, Suvaporn Anugulruengkit, Rujipat Samransamruajkit, Darintr Sosothikul, Ornanong Tansrijitdee, Anry Nakphunsung, Patchareeyawan Srimuan, Jirachaya Sophonphan, ThanyaweePuthanakit, Kunyanut Payuk, Wilawan Picheansathian, Nongkran Viseskul, Elizabeth DeNardo, Rachel Leslie, Todd Cartner, Luciana Barbosa, Heinz-Peter Werner, Florian H. H. Brill, Julia Yaeko Kawagoe, Elizabeth De Nardo, Sarah Edmonds- Wilson, David Macinga, Patricia Mays-Suko, Collette Duley, Tran Thi Thuy Hang, Tran Thi My Hanh, Christopher Gordon, Roopa Durairaj, Anusha Rohit, Saujanya Saravanakumar, Jothymani Hemalatha, Ryuichi Hirano, Yuichi Sakamoto, Shoji Yamamoto, Naoki Tachibana, Miho Miura, Fumiyo Hieda, Yoshiro Sakai, Hiroshi Watanabe, Silverose Ann Bacolcol, Keitleen Chavez, Jia-Wei Lim, Aung-Aung Hein, Grace Tin, Vanessa Lim, Huwi-chun Chao, Chiu-Yin Yeh, Mei-feng Lo, Chonlada Piwpong, Songyos Rajborirug, Ploypailin Preechawetchakul, Yada Pruekrattananapa, Tharntip Sangsuwan, Ratchanee Wongsaen, Sungwan Paengta, Napatnun Nilchon, Sutthipun Thanompan, Samattanet Tariyo, Svetlana Kolesnichenko, Yerbol Tishkambayev, Asylkhan Alibecov, Yekaterina Serbo, Youngwon Nam, Jae Hyeon Park, Yun Ji Hong, Taek Soo Kim, Jeong Su Park, Kyoung Un Park, Eui-Chong Kim, Samuel Abumhere Aziegbemhin, Onaiwu Enabulele, Yao-Shen Tung, An-Chi Chen, Shen-Min Huang, Yui-Yein Yang, Li-Hung Wu, Chin-cheng Lin, Tzu Hao Lien, Jia Hao Chang, Yu Shan Huang, Yi Shun Chen, Sasithorn Sirilun, Phisit Ouirungroj, Suwanna Trakulsomboon, Patcharee Prasajak, Maryanne W. N. Kwok, Lady S. H. Ng, Lindy M. T. Wong, Lenina S. L. Poon, Mary K. L. Lai, Holly H. S. Cheng, S. K. Fong, Cindy F. Y. Leung, Jumpei Hasegawa, Hiroki Shirakawa, Sachiko Wakai, Makiko Mieno, Shuji Hatakeyama, Manu Deeudom, Prasit Tharavichitkul, Terrence Chinniah, Jackson Tan, Kavitha Prabu, Sartaj Alam, Aung Kyaw Wynn, Rashidah Ahmad, Amalina Sidek, Dg Azizah Samsuddin, Noraini Ajis, Aliyah Ahmad, Susylawathi Magon, Boon Chu, Jiqiu Kuang, Yan Gao, Shoujun Wang, Yunxiao Hao, Rong Liu, Dongmei Li, Hui Wang, Hisanori Nishio, Hitomi Mori, Yoshiko Morokuma, Takaaki Yamada, Makiko Kiyosuke, Sachie Yasunaga, Kazuhiro Toyoda, Nobuyuki Shimono, Dmitriy Babenko, Anar Turmuhambetova, Antonella Cheşcă, Mark A. Toleman, Lyudmila L. Akhmaltdinova, Mark Albert Magsakay, Angelo Macatibag, Jeannica Kriselle Lerios, Alyona Lavrineko, Dmitry Babenko, Eugene Sheck, Mikhail Edelstein, Lih-Yue Li, Chiung-Wen Chan, Hui-Chuan Pan, Wipa Vanishakije, Warisra Jaikampun, Su-Yin Li, Jian-Feng Li, Yu-Ping Wu, Chiao-Hui Lin, Ping-Chin Chang, Samatanet Tariyo, Suttsiphan Thanompan, Suchada Sukkra, Khalequ Zaman, Sheikh Farzana Zaman, Farzana Zaman, Asma Aziz, Sayeed-Bin Faisal, Magali Traskine, Javier Ruiz-Guiñazú, Dorota Borys, Wendy Wai Yee Lam, May Chow, Lucy Choy, Joseph Kam, Sharifah Azura Salleh, Razila Yacob, Siti Rokiah Yusof, Nordiah Awang Jalil, Maria Lourdes Millan, Jose Lito Acuin, Melecia A. Velmonte, Silverose Ann A. Bacolcol, Ching-I Ting, Sunisa Dissayasriroj, Terrence Rohan Chinniah, Jauharatud DiniSuhaimi, Aizzuddin Mirasin, Nurul Morni, Azizah Samsuddin, Amalina AbuBakar, Amanie Shafiee, Julaini Safar, Leung Annie, Fung Yuk Ling, Lau Edna, Luk Kristine, Satoshi Shinomiya, Kumiko Yamamoto, Kayoko Kjiwara, Mitsuhiro Yamaguchi, Wei Zhang, Bee-Fong Poh, Ming-Chin Chan, Chih-Chien Wang, Huan-Yu Huang, Chiung-Ling Lai, Sajeerat Kosol, Wantana Sakolwirat, Patchanee Paepong, Sawalee Jansanga, Pattarin Jaisamoot, Nuttha Thongnuanual, Chittima Srithong, Somporn Somsakul, Sutima Plongpunth, Mukkapon Punpop, Porntip Malathum, Kulada Peautiwat, Nattawipa boon kirdram, Pimpaporn Klunklin, Geetha Samethadka, Naoko Suzuki, Hitomi Asada, Masao Katayama, Atsushi Komano, Hidehiro Watanabe, Hye Kyung Seo, Joo-Hee Hwang, Myoung Jin Shin, Su Young Kim, Eu Suk Kim, Kyoung-Ho Song, Hong Bin Kim, Lai-Si Un, Choi-Ian Vong, Jocelyn Koh, Sherly Agustinus, Rozita Bte Abu Hassan, Yin Phyu Thinn, Benjamin Ng, Soe Pyae Tun, Su Mon Thi Ha, Xue Xiaoting, Lin Li, Leyland Chuang, Attanayaka Mudiyanselage Chulani Niroshika, Kaluarachchige Anoma Kaluarachchi Perera, Dimingo Kankanamalage Diana Grace Fernando, Bodhipakshage Rohini Hemamala, Chiu-yin Yeh, Hui-Chun Yang, Hsiang-Ju Chiu, Ya-Ling Shih, Yu-Shan Chien, Wan-Yi Lin, Chia-Yun Pan, Ying-Yun Chang, Chiu-Yuch Yea, Ming-Hsien Chu, Li-Chu Lee, Lin Yu-Hsiu, Guo Siao-Pei, Leung Pak-On, Sie Mei-Fe, Chen Jyh-Jou, Chang Yong-Yuan, Shu-Yuan Kuo, Yu-Hsiu Lin, Ji-Sheng Zhang, Pak-On Leung, Mei-Fe Sie, Jyh-Jou Chen, Yan-Ru Chen, Ying-Ling Chen, Chi-Fen Taou, Hsiao-Shan Chen, Hung-Jen Tang, Shin Yu Chen, Yin Yin Chen, Fu Der Wang, Tzu-Ping Shih, Chin-Yu Chen, Su-Jung Chen, Mei-chi Wu, Wan-ju Yang, Mei-ling Chou, Man-Ling Yu, Li-Chu Li, Cheng-Wei Chu, Wen-Hao Tsou, Wen-Chih Wu, Wen-Chi Cheng, Cho-Ching Sun, Shu-Hua Lu, Hsin-Ling Yang, Cheng-Yu Lu, Nitchawan Hirunprapakorn, Sirilux Apivanich, Ttipakorn Pornmee, Chonnikarnt Beowsomboon, Itthaporn Kumkoom, Nongyao Kasatpibal, Jittaporn Chitreecheur, JoAnne D. Whitney, Surasak Saokaew, Kirati Kengkla, Margaret M. Heitkemper, Thanomvong Muntajit, Siriluk Apivanich, Hang Thi Phan, Anh Pham Phuong Dinh, and Tuyet Thi Kim Nguyen
- Subjects
Infectious and parasitic diseases ,RC109-216 - Published
- 2017
- Full Text
- View/download PDF
8. Tigecycline resistance among carbapenem-resistant Klebsiella Pneumoniae: Clinical characteristics and expression levels of efflux pump genes.
- Author
-
Sheng-Kang Chiu, Ming-Chin Chan, Li-Yueh Huang, Yi-Tsung Lin, Jung-Chung Lin, Po-Liang Lu, L Kristopher Siu, Feng-Yee Chang, and Kuo-Ming Yeh
- Subjects
Medicine ,Science - Abstract
Tigecycline is a treatment option for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP). Emerging tigecycline resistance in CRKP represents a growing threat. Knowledge of the clinical, microbiological, and molecular characteristics of tigecycline- and carbapenem-resistant Klebsiella pneumoniae (TCRKP) is limited.Patients infected with TCRKP were identified from a Taiwanese national surveillance study. Clinical data were collected from medical records. We performed susceptibility tests, carbapenemase gene detection, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Furthermore, we performed quantitative real-time polymerase chain reaction (qRT-PCR) analyses to assess the expression levels of the efflux pump genes acrB and oqxB.We identified 16 patients infected with TCRKP, with urinary tract infection (UTI) being the most common type of infection (63%). The all-cause 30-day mortality rate was 44% in patients with TCRKP infection. Patients with a site of infection other than the urinary tract had a significantly higher mortality rate than patients with UTIs (83% vs. 20%, p = 0.035). PFGE and MLST revealed no dominant clone or sequence type. Using qRT-PCR, overexpression of both the acrB and oqxB genes was identified in seven isolates, and overexpression of the oqxB gene was observed in another seven. There was poor correlation between acrB or oqxB expression and tigecycline MICs (r = -0.038 and -0.166, respectively).The mortality rate in patients infected with TCRKP in this study was 44% and this is an important subset of patients. The absence of a linear relationship between efflux pump genes expression and MICs indicates that tigecycline resistance may be mediated by other factors. Continuous monitoring of tigecycline resistance among CRKP isolates and resistance mechanisms are necessary.
- Published
- 2017
- Full Text
- View/download PDF
9. Risk factors for healthcare-associated extensively drug-resistant Acinetobacter baumannii infections: a case-control study.
- Author
-
Ming-Chin Chan, Sheng-Kang Chiu, Po-Ren Hsueh, Ning-Chi Wang, Chih-Chien Wang, and Chi-Tai Fang
- Subjects
Medicine ,Science - Abstract
The emergence of extensively drug-resistant Acinetobacter baumannii (XDRAB) is a serious threat to hospitalized patients. From 2008 to 2010, surveillance detected 25 hospital-acquired infection (HAI) cases caused by XDRAB at a medical center in Taipei. The site of XDRAB infection was bloodstream (n = 8), urinary tract (n = 12), lower respiratory tract (n = 3), surgical site (n = 1), and cardiovascular (n = 1). The isolates were resistant to all currently available antibiotics except for colistin. The XDRAB isolates are genetically diverse, shown by pulsed-field gel electrophoresis, but 23 of 25 harbored class 1 integron with a 2.3-kb gene cassette. Most of these isolates carry OXA-23 (n = 21) and OXA-51-like carbapenemase genes (n = 25). To identify the risk factors, a case-control study was conducted. The 25 cases were compared with 100 controls randomly selected from hospitalized patients without XDRAB-HAIs, matched by the onset date, ward, and age, at a ratio of 1∶4. Prior use of imipenem, meropenem, piperacillin/tazobactam or fourth-generation cephalosporins (adjusted OR: 3.2, 95% CI: 1.03-10.2, P = 0.04) and >30 days bed-ridden (adjusted OR: 6.0, 95% CI: 1.3-27.6, P = 0.02) were found to be the independent risk factors for XDRAB-HAIs. These findings highlight that, even in the absence of clonal dissemination, XDRAB can emerge under the selective pressure of broad-spectrum antibiotics and causes subsequent HAIs in compromised hosts. An appropriate response to the XDRAB threat therefore should include a component of prudent use of broad-spectrum antibiotics active against gram-negative bacteria.
- Published
- 2014
- Full Text
- View/download PDF
10. A truncated AdeS kinase protein generated by ISAba1 insertion correlates with tigecycline resistance in Acinetobacter baumannii.
- Author
-
Jun-Ren Sun, Cherng-Lih Perng, Ming-Chin Chan, Yuji Morita, Jung-Chung Lin, Chih-Mao Su, Wei-Yao Wang, Tein-Yao Chang, and Tzong-Shi Chiueh
- Subjects
Medicine ,Science - Abstract
Over-expression of AdeABC efflux pump stimulated continuously by the mutated AdeRS two component system has been found to result in antimicrobial resistance, even tigecycline (TGC) resistance, in multidrug-resistant Acinetobacter baumannii (MRAB). Although the insertion sequence, ISAba1, contributes to one of the AdeRS mutations, the detail mechanism remains unclear. In the present study we collected 130 TGC-resistant isolates from 317 carbapenem resistant MRAB (MRAB-C) isolates, and 38 of them were characterized with ISAba1 insertion in the adeS gene. The relationship between the expression of AdeABC efflux pump and TGC resistant was verified indirectly by successfully reducing TGC resistance with NMP, an efflux pump inhibitor. Further analysis showed that the remaining gene following the ISAba1 insertion was still transcribed to generate a truncated AdeS protein by the Pout promoter on ISAba1 instead of frame shift or pre-termination. Through introducing a series of recombinant adeRS constructs into a adeRS knockout strain, we demonstrated the truncated AdeS protein was constitutively produced and stimulating the expression of AdeABC efflux pump via interaction with AdeR. Our findings suggest a mechanism of antimicrobial resistance induced by an aberrant cytoplasmic sensor derived from an insertion element.
- Published
- 2012
- Full Text
- View/download PDF
11. Clinical Application of Ultraviolet C Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 in Contaminated Hospital Environments
- Author
-
Ming-Yieh Peng, Ming-Chin Chan, Mei-Chen Yang, Wen-Lin Su, Sheg-Kang Chiu, You-Chen Chao, Chih-Pei Lin, Hui-Ching Huang, and Yao-Kuang Wu
- Subjects
Time Factors ,Coronavirus disease 2019 (COVID-19) ,Ultraviolet Rays ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,RT-PCR ,Microbiology ,Article ,environmental sampling ,Virology ,Medicine ,Humans ,Irradiation ,Infection Control ,business.industry ,SARS-CoV-2 ,Uvc irradiation ,Healthcare worker ,COVID-19 ,Dose-Response Relationship, Radiation ,Contamination ,254 nm Ultraviolet C ,Hospitals ,QR1-502 ,Contamination rate ,Infectious Diseases ,business - Abstract
To overcome the ongoing coronavirus disease 2019 (COVID-19) pandemic, transmission routes, such as healthcare worker infection, must be effectively prevented. Ultraviolet C (UVC) (254 nm) has recently been demonstrated to prevent environmental contamination by infected patients; however, studies on its application in contaminated hospital settings are limited. Herein, we explored the clinical application of UVC and determined its optimal dose. Environmental samples (n = 267) collected in 2021 were analyzed by a reverse transcription-polymerase chain reaction and subjected to UVC irradiation for different durations (minutes). We found that washbasins had a high contamination rate (45.5%). SARS-CoV-2 was inactivated after 15 min (estimated dose: 126 mJ/cm2) of UVC irradiation, and the contamination decreased from 41.7% before irradiation to 16.7%, 8.3%, and 0% after 5, 10, and 15 min of irradiation, respectively (p = 0.005). However, SARS-CoV-2 was still detected in washbasins after irradiation for 20 min but not after 30 min (252 mJ/cm2). Thus, 15 min of 254-nm UVC irradiation was effective in cleaning plastic, steel, and wood surfaces in the isolation ward. For silicon items, such as washbasins, 30 min was suggested; however, further studies using hospital environmental samples are needed to confirm the effective UVC inactivation of SARS-CoV-2.
- Published
- 2021
12. A familial COVID-19 cluster and quarantine strategy shift in the first month in Taiwan
- Author
-
Jen Chun Wang, Shih-Hung Tsai, Hung Sheng Shang, Po Chuan Chen, Jung Chung Lin, and Ming Chin Chan
- Subjects
2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,law ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Quarantine ,Emergency Medicine ,Medicine ,Disease cluster ,business ,Virology ,law.invention - Published
- 2020
13. Surveillance for coronavirus diseases 2019 (COVID-19) among health care workers at a medical center in Taiwan, March to August 2020
- Author
-
Jung-Chung Lin, Feng-Yee Chang, Ming Chin Chan, and Tzu Jou Cho
- Subjects
2019-20 coronavirus outbreak ,medicine.medical_specialty ,lcsh:R5-920 ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Health Personnel ,Taiwan ,COVID-19 ,General Medicine ,medicine.disease_cause ,Article ,Hospitals ,Health personnel ,Family medicine ,Health care ,Epidemiological Monitoring ,Medicine ,Humans ,Center (algebra and category theory) ,business ,lcsh:Medicine (General) ,Coronavirus - Published
- 2021
14. Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012–2015
- Author
-
Chang-Phone Fung, Yi Tsung Lin, Jann-Tay Wang, Po-Liang Lu, Sheng Kang Chiu, Kuo Ming Yeh, Yin Ching Chuang, Tsu Lan Wu, Ming Chin Chan, Jung-Chung Lin, and Ling Ma
- Subjects
0301 basic medicine ,DNA, Bacterial ,Imipenem ,Carbapenem ,Klebsiella pneumoniae ,030106 microbiology ,Taiwan ,lcsh:Medicine ,Drug resistance ,Aztreonam ,Microbial Sensitivity Tests ,Meropenem ,Article ,beta-Lactamases ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Antibiotic resistance ,Bacterial Proteins ,Drug Resistance, Bacterial ,polycyclic compounds ,medicine ,Prevalence ,Humans ,lcsh:Science ,Multidisciplinary ,biology ,lcsh:R ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Hospitals ,Anti-Bacterial Agents ,Klebsiella Infections ,chemistry ,Multilocus sequence typing ,lcsh:Q ,medicine.drug ,Fluoroquinolones ,Multilocus Sequence Typing - Abstract
Before 2011, the prevalence rates of carbapenemase-producing Klebsiella pneumoniae (CPKP) among carbapenem nonsusceptible K. pneumoniae (CnSKP) isolates were below 10% in Taiwan. The study presents the dissemination and increased antimicrobial resistance of CPKP from January 2012 to August 2015, as shown by Taiwanese multicenter surveillance. Isolates with minimum inhibitory concentrations (MICs) of >1 μg/mL for imipenem or meropenem were collected, screened for various carbapenemase genes by PCR, and tested for antimicrobial susceptibility. Among 1,457 CnSKP isolates, 1,250 were collected from medical centers. The CnSKP prevalence in medical centers increased by 1.7-fold during the study. Among all CnSKP isolates, 457 were CPKP. The CPKP rate among CnSKP increased by 1.5-fold and reached 36.8% in 2015. The CPKP nonsusceptibility rate to aztreonam, fluoroquinolones, and aminoglycosides increased yearly. Six CPKP isolates carried dual carbapenemase genes. Three Ambler classes were identified in 451 isolates with a single carbapenemase: classes A (315 blaKPC-2, 2 blaKPC-3, 28 blaKPC-17, 2 blaKPC-34), B (26 blaIMP-8, 2 blaNDM-1, 36 blaVIM-1), and D (40 blaOXA-48). The blaOXA-48 rate among CPKP increased by 6-fold over three years. Most KPC and OXA-48 producers were ST11. CnSKP was increasingly prevalent, owing to CPKP dissemination. Additionally, CPKP became more resistant during the study period.
- Published
- 2018
15. Erratum to: Abstracts from the 8th International Congress of the Asia Pacific Society of Infection Control (APSIC)
- Author
-
Wilawan Picheansathian, Rocio Alvarez Marin, Rashidah Ppha. Ahmad, Marta Aires-de-Sousa, Pimpaporn Klunklin, Hang Thi Phan, Wison Laochareonsuk, Woranan Boonsomsuk, Tuyet Thi Kim Nguyen, Jauharatud DiniSuhaimi, Aizzuddin Mirasin, Ming-Chin Chan, Nicolas Kieffer, Chollathip Bunyaphongphan, Amalina Sidek, Nattawipa Boonkirdram, Amalina AbuBakar, Kavitha Prabu, Anh Pham Phuong Dinh, Pawin Wanasitchaiwat, Noraini Ajis, Laurent Poirel, Azizah Samsuddin, Chih-Chien Wang, Marta Aires de Sousa, Amanie Shafiee, Silom Jamulitrat, Patrice Nordmann, Sutasinee Thana, Terrence Rohan Chinniah, Pakpoom Maneepongpermpoon, Julaini Safar, Sireekul Petyu, Boon Chu, Nurul Morni, Hermínia de Lencastre, Susylawathi Magon, Teresa Conceição, and Aliyah Ahmad
- Subjects
0301 basic medicine ,Microbiology (medical) ,business.industry ,030106 microbiology ,Public Health, Environmental and Occupational Health ,Library science ,Physiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Asia pacific ,International congress ,Infection control ,Medicine ,Pharmacology (medical) ,lcsh:RC109-216 ,030212 general & internal medicine ,Erratum ,business - Published
- 2017
16. Clinical manifestations and prognostic factors of Morganella morganii bacteremia
- Author
-
Yi Chao Lee, Feng-Yee Chang, Tzu-Chao Lin, Kuo-Ming Yeh, Jung-Chung Lin, Ming-Chin Chan, and Ya-Sung Yang
- Subjects
Male ,Microbiology (medical) ,medicine.medical_specialty ,Urinary system ,Taiwan ,Penicillanic Acid ,Bacteremia ,Microbial Sensitivity Tests ,Gastroenterology ,law.invention ,Risk Factors ,law ,Internal medicine ,Drug Resistance, Bacterial ,medicine ,Humans ,Aged ,Aged, 80 and over ,Morganella morganii ,Piperacillin ,Cross Infection ,Univariate analysis ,biology ,APACHE II ,business.industry ,Age Factors ,Enterobacteriaceae Infections ,General Medicine ,Odds ratio ,Middle Aged ,biology.organism_classification ,medicine.disease ,Intensive care unit ,Anti-Bacterial Agents ,Cephalosporins ,Surgery ,Community-Acquired Infections ,Ciprofloxacin ,Piperacillin, Tazobactam Drug Combination ,Infectious Diseases ,Ampicillin ,Female ,Gentamicins ,business ,medicine.drug - Abstract
Although Morganella morganii causes a variety of clinical infections, there are limited studies on M. morganii bacteremia after the year 2000. A total of 109 patients with M. morganii bacteremia at a medical center in Taiwan from 2003 to 2012 were studied. Among them, 30.3 % had polymicrobial bacteremia and 75.2 % had community-acquired infection. The most common underlying diseases were hypertension (62.4 %) and diabetes mellitus (38.5 %). The urinary tract (41.3 %) was the major portal of entry, followed by the hepatobiliary tract (27.5 %), skin and soft tissue (21.1 %), and primary bacteremia (10.1 %). Susceptibility testing of M. morganii isolates showed ubiquitous resistance to first-generation cephalosporins and ampicillin–clavulanate; resistance rates to gentamicin, piperacillin–tazobactam, and ciprofloxacin were 30.3 %, 1.8 %, and 10.1 %, respectively. Overall, the 14-day mortality was 14.7 %. Univariate analysis revealed that elevated blood urea nitrogen (BUN) values [p = 0.0137, odds ratio (OR) 5.26], intensive care unit (ICU) admission (p = 0.011, OR 4.4), and higher Acute Physiology and Chronic Health Evaluation II (APACHE II) scores (p
- Published
- 2014
17. AdeRS combination codes differentiate the response to efflux pump inhibitors in tigecycline-resistant isolates of extensively drug-resistant Acinetobacter baumannii
- Author
-
F.-M. Lin, Tein-Yao Chang, Cherng-Lih Perng, Jung-Chung Lin, Jun-Ren Sun, Ming-Chin Chan, Tzong-Shi Chiueh, and Ya-Sung Yang
- Subjects
Acinetobacter baumannii ,Microbiology (medical) ,Minocycline ,Microbial Sensitivity Tests ,Tigecycline ,Drug resistance ,Biology ,Group A ,Microbiology ,Minimum inhibitory concentration ,Bacterial Proteins ,Drug Resistance, Multiple, Bacterial ,medicine ,Pulsed-field gel electrophoresis ,Humans ,Typing ,Membrane Transport Proteins ,General Medicine ,biology.organism_classification ,Anti-Bacterial Agents ,Electrophoresis, Gel, Pulsed-Field ,Infectious Diseases ,Efflux ,Acinetobacter Infections ,medicine.drug - Abstract
Tigecycline (TGC)-resistant extensively drug-resistant Acinetobacter baumannii (XDRAB) is an increasing threat in regard to nosocomial infections. The resistance-nodulation-cell division (RND) efflux pump has played an important role in TGC resistance. In this study, total 81 TGC-resistant XDRAB isolates were analyzed for their responses to the efflux pump inhibitor 1-(1-naphthylmethyl)-piperazine (NMP). We found that NMP could reduce by 4-fold or greater than 4-fold the minimum inhibitory concentration (MIC) of TGC in 45 isolates (55.6 %). After typing with pulsed-field gel electrophoresis (PFGE), group A appeared to be the major cluster with good synergistic response to NMP. Transcripts of the AdeABC efflux pump gene were consistently more correlated with TGC resistance than transcripts of the AdeFGJ or AdeIJK efflux pump genes in these isolates. Of the 81 isolates, the amino acid sequences of AdeR and AdeS were further classified and combined into 31 different codes. Although the dissemination of TGC-resistant XDRAB isolates was genetically diverse in our hospital, their responses to NMP conversion were still strain-dependent. We found that AdeRS combination codes were better than PFGE typing in separating groups of isolates with different sensitivity to NMP conversion.
- Published
- 2014
18. Applying ATP bioluminescence to design and evaluate a successful new intensive care unit cleaning programme
- Author
-
T.-L. Liu, C.-M. Chang, Ming-Chin Chan, Sheng-Kang Chiu, Y.-H. Chiu, P.-S. Hung, T.-F. Huang, Te-Yu Lin, and Jung-Chung Lin
- Subjects
Microbiology (medical) ,Healthcare associated infections ,medicine.medical_specialty ,Health Personnel ,Taiwan ,law.invention ,law ,Humans ,Medicine ,Prospective Studies ,Disposable Equipment ,Intensive care medicine ,Training programme ,Equipment and Supplies, Hospital ,Academic Medical Centers ,Cross Infection ,business.industry ,Housekeeping, Hospital ,General Medicine ,Baseline data ,Intensive care unit ,Intervention studies ,Atp bioluminescence ,Infection rate ,Disinfection ,Intensive Care Units ,Infectious Diseases ,Medical intensive care unit ,Luminescent Measurements ,Emergency medicine ,Equipment Contamination ,Cardiology Service, Hospital ,business - Abstract
This was a two-phase prospective intervention study in the cardiology intensive care unit (CICU) and medical intensive care unit (MICU) and of a public 1800-bed medical centre in Taiwan. In phase I, cleaning efficacy was monitored by ATP bioluminescence after daily morning cleaning, and only 43.9% of 221 tested surfaces passed. The baseline data were used to define an intervention consisting of a new cleaning protocol as well as a new education/training programme. In phase II, following the intervention, 88.1% of 270 surfaces were found to be clean. The combined infection rate in the CICU and MICU showed a statistically significant decrease of 49.7%.
- Published
- 2015
19. AdeR protein regulates adeABC expression by binding to a direct-repeat motif in the intercistronic spacer
- Author
-
Bo-Jun Huang, Jun-Ren Sun, Cherng-Lih Perng, Tzong-Shi Chiueh, Cheng-Ping Yu, Ming-Chin Chan, and Tein-Yao Chang
- Subjects
0301 basic medicine ,Acinetobacter baumannii ,DNA, Bacterial ,Electrophoresis ,Operon ,030106 microbiology ,Mutant ,Amino Acid Motifs ,Minocycline ,Microbial Sensitivity Tests ,Microbiology ,Tigecycline ,03 medical and health sciences ,Direct repeat ,Repetitive Sequences, Nucleic Acid ,Genetics ,biology ,Base Sequence ,Tetracycline Resistance ,Chromosome Mapping ,Membrane Transport Proteins ,Spacer DNA ,biology.organism_classification ,Two-component regulatory system ,Anti-Bacterial Agents ,Gene cassette ,Mutation ,Efflux ,Sequence Analysis ,Gene Deletion - Abstract
Overexpression of the efflux pump AdeABC is associated with tigecycline resistance of multi-drug resistant Acinetobacter baumannii (MDRAB). A two-component regulatory system, sensor AdeS and regulator AdeR proteins regulate the pump. However, the detailed mechanism of the AdeR protein to enhance the expression of adeABC operon is not well defined. We illustrated the biological characteristics of AdeR proteins by comparing a mutant AdeR protein of a tigecycline resistant MDRAB to the wild AdeR protein. By analyzing a series of deletion constructs, a minimal gene cassette of the intercistronic spacer DNA fragment specifically bound with the adeR protein and resulted in band shifting in electrophoresis mobility shifting assays (EMSA). A conserve direct repeat motif was observed in the intercistronic spacer DNA. We demonstrated the AdeR protein was a direct-repeat-binding protein. Two common residue mutations on the AdeR proteins of tigecycline resistant MDRAB isolates could reduce their binding affinity with the intercistronic spacer. The free intercistronic spacer may then more efficiently support the read-through of the adeABC operon during the co-transcriptional translation in tigecycline resistant MDRAB isolates.
- Published
- 2015
20. Efficacy evaluation of automatic hydrogen peroxide dry mist system on healthcare environment disinfection
- Author
-
Tzu-Feng Huang, Feng-Yee Chang, Ming-Chin Chan, and Ching-Mei Chang
- Subjects
Microbiology (medical) ,chemistry.chemical_compound ,Infectious Diseases ,General Immunology and Microbiology ,chemistry ,Immunology and Microbiology(all) ,Mist ,Environmental science ,Immunology and Allergy ,General Medicine ,Hydrogen peroxide ,Pulp and paper industry - Published
- 2015
- Full Text
- View/download PDF
21. Tigecycline resistance among carbapenem-resistant Klebsiella Pneumoniae: Clinical characteristics and expression levels of efflux pump genes
- Author
-
Yi Tsung Lin, Sheng Kang Chiu, Kuo Ming Yeh, L. Kristopher Siu, Po-Liang Lu, Feng-Yee Chang, Ming Chin Chan, Jung-Chung Lin, and Li Yueh Huang
- Subjects
Male ,Bacterial Diseases ,0301 basic medicine ,Klebsiella pneumoniae ,Antibiotics ,lcsh:Medicine ,Gene Expression ,Minocycline ,Drug resistance ,Tigecycline ,Pathology and Laboratory Medicine ,Klebsiella Pneumoniae ,law.invention ,Geographical Locations ,law ,Klebsiella ,Medicine and Health Sciences ,Medicine ,lcsh:Science ,Polymerase chain reaction ,Aged, 80 and over ,Multidisciplinary ,biology ,Antimicrobials ,Drugs ,Middle Aged ,Anti-Bacterial Agents ,Bacterial Pathogens ,Infectious Diseases ,Medical Microbiology ,Urinary Tract Infections ,Hyperexpression Techniques ,Female ,Efflux ,Pathogens ,Research Article ,medicine.drug ,Asia ,Death Rates ,medicine.drug_class ,Urology ,030106 microbiology ,Taiwan ,Real-Time Polymerase Chain Reaction ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Microbial Control ,Drug Resistance, Bacterial ,Gene Expression and Vector Techniques ,Genetics ,Pulsed-field gel electrophoresis ,Humans ,Molecular Biology Techniques ,Microbial Pathogens ,Molecular Biology ,Aged ,Demography ,Pharmacology ,Molecular Biology Assays and Analysis Techniques ,Bacteria ,business.industry ,lcsh:R ,Organisms ,Biology and Life Sciences ,Biological Transport ,biology.organism_classification ,Klebsiella Infections ,Carbapenems ,Genes, Bacterial ,People and Places ,Multilocus sequence typing ,lcsh:Q ,business - Abstract
Objectives Tigecycline is a treatment option for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP). Emerging tigecycline resistance in CRKP represents a growing threat. Knowledge of the clinical, microbiological, and molecular characteristics of tigecycline- and carbapenem-resistant Klebsiella pneumoniae (TCRKP) is limited. Methods Patients infected with TCRKP were identified from a Taiwanese national surveillance study. Clinical data were collected from medical records. We performed susceptibility tests, carbapenemase gene detection, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Furthermore, we performed quantitative real-time polymerase chain reaction (qRT-PCR) analyses to assess the expression levels of the efflux pump genes acrB and oqxB. Results We identified 16 patients infected with TCRKP, with urinary tract infection (UTI) being the most common type of infection (63%). The all-cause 30-day mortality rate was 44% in patients with TCRKP infection. Patients with a site of infection other than the urinary tract had a significantly higher mortality rate than patients with UTIs (83% vs. 20%, p = 0.035). PFGE and MLST revealed no dominant clone or sequence type. Using qRT-PCR, overexpression of both the acrB and oqxB genes was identified in seven isolates, and overexpression of the oqxB gene was observed in another seven. There was poor correlation between acrB or oqxB expression and tigecycline MICs (r = -0.038 and -0.166, respectively). Conclusions The mortality rate in patients infected with TCRKP in this study was 44% and this is an important subset of patients. The absence of a linear relationship between efflux pump genes expression and MICs indicates that tigecycline resistance may be mediated by other factors. Continuous monitoring of tigecycline resistance among CRKP isolates and resistance mechanisms are necessary.
- Published
- 2017
22. Comparisons between patients with trimethoprim-sulfamethoxazole-susceptible and trimethoprim-sulfamethoxazole-resistant Stenotrophomonas maltophilia monomicrobial bacteremia: A 10-year retrospective study
- Author
-
Kuo-Ming Yeh, Feng-Yee Chang, Ya-Sung Yang, Li-Ping Kan, Chin-Hsuan Yang, Hsin-An Lin, Ning-Chi Wang, Sheng-Kang Chiu, Te-Yu Lin, Ming-Chin Chan, Jung-Chung Lin, and Ching-Hsun Wang
- Subjects
0301 basic medicine ,Microbiology (medical) ,Male ,medicine.medical_specialty ,Stenotrophomonas maltophilia ,030106 microbiology ,Bacteremia ,Microbial Sensitivity Tests ,resistance ,03 medical and health sciences ,trimethoprim–sulfamethoxazole ,Immunology and Microbiology(all) ,Internal medicine ,Drug Resistance, Multiple, Bacterial ,Trimethoprim, Sulfamethoxazole Drug Combination ,medicine ,Immunology and Allergy ,Humans ,Hospital Mortality ,Risk factor ,APACHE ,Aged ,Retrospective Studies ,General Immunology and Microbiology ,biology ,Septic shock ,business.industry ,Incidence (epidemiology) ,Sulfamethoxazole ,Mortality rate ,General Medicine ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,Trimethoprim ,Shock, Septic ,Surgery ,Anti-Bacterial Agents ,Hospitalization ,Infectious Diseases ,Treatment Outcome ,Female ,business ,Gram-Negative Bacterial Infections ,medicine.drug - Abstract
Background/purpose The impact of bacteremia due to the resistance of Stenotrophomonas maltophilia to trimethoprim–sulfamethoxazole (TMP–SXT) is uncertain. This study compared the clinical characteristics and outcomes of patients with TMP–SXT-susceptible (TSSSM) and TMP–SXT-resistant S. maltophilia (TSRSM) monomicrobial bacteremia. Methods The medical records of adult patients with TSSSM and TSRSM monomicrobial bacteremia from January 2004 to December 2013 were reviewed and classified into two groups, namely, TSSSM and TSRSM. Results There were 184 patients with monomicrobial S. maltophilia bacteremia. The mean age was 68.3 years. Most patients were males (72.8%), had high Charlson Comorbidity Index scores, previously prescribed antimicrobial agents, and indwelling medical devices. The 14-day and in-hospital mortality rates were 23.9% and 47.2%, respectively. There were 128 patients (69.6%) with TSSSM and 56 (30.4%) with TSRSM. The incidence of TSSSM bacteremia increased during the study period. The TSSSM and TSRSM groups had similar demographic and clinical characteristics and no significant differences in 14-day and in-hospital mortality (24.2% vs. 23.2%, p = 0.833; 50.0% vs. 41.1%, p = 0.264, respectively). Patients with TSSSM bacteremia had an increased risk of septic shock ( p = 0.044) and neutropenia ( p = 0.028) at bacteremia onset. Logistic regression analysis indicated that acquisition of TMP–SXT resistance was an independent risk factor for prolonged hospitalization ( p = 0.018) and catheter-related S. maltophilia bacteremia was inversely associated with prolonged hospitalization after bacteremia ( p = 0.032). Conclusion There were no significant differences in mortality for patients with TSSSM and TSRSM bacteremia, but patients with TSRSM bacteremia were associated with prolonged hospitalization after bacteremia onset.
- Published
- 2014
23. Molecular typing of healthcare-associated infection strains with random amplified polymorphic DNA method by two types of capillary electrophoresis analysis
- Author
-
Tzu-Feng Huang, Xin-Yi Zhong, Xiao-Wei Li, Tzong-Shi Chiueh, Yi-Hui Wang, Ming-Chin Chan, Ming-Zhi Jian, and Cherng-Lih Perng
- Subjects
Healthcare associated infections ,Microbiology (medical) ,General Immunology and Microbiology ,General Medicine ,Biology ,Molecular biology ,body regions ,Molecular typing ,chemistry.chemical_compound ,Capillary electrophoresis ,Infectious Diseases ,chemistry ,Immunology and Microbiology(all) ,Cleaved amplified polymorphic sequence ,embryonic structures ,Immunology and Allergy ,DNA - Published
- 2015
- Full Text
- View/download PDF
24. Candida lipolytica candidemia as a rare infectious complication of acute pancreatitis: a case report and literature review
- Author
-
Ming-Chin Chan, Te-Yu Lin, Sheng-Kang Chiu, Chin-Hui Hsu, and Wen-Cheng Liu
- Subjects
Microbiology (medical) ,Antifungal ,Male ,medicine.medical_specialty ,medicine.drug_class ,medicine.medical_treatment ,Pathogenesis ,Opportunistic pathogen ,fluids and secretions ,Infectious complication ,Immunology and Microbiology(all) ,hemic and lymphatic diseases ,Candida lipolytica ,medicine ,Immunology and Allergy ,Humans ,Intensive care medicine ,Candida ,Aged, 80 and over ,General Immunology and Microbiology ,business.industry ,Pancreatitis, Acute Necrotizing ,Candidemia ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,equipment and supplies ,bacterial infections and mycoses ,medicine.disease ,Acute pancreatitis ,Clinical Practice ,Infectious Diseases ,business ,Central venous catheter - Abstract
Candida lipolytica candidemia is a rare but an emerging pathogenic yeast infection in humans. It can gain access to the bloodstream through intravascular catheterization, especially through central venous catheters in immunocompromised or critically ill patients during hospitalization. In this report, we present a noncatheter-related C. lipolytica candidemia infection in an 84-year-old man who was admitted due to acute pancreatitis. The possible pathogenesis and management of C. lipolytica candidemia are highlighted. It was an unusual infectious complication of acute pancreatitis. Clinicians should be aware that such an opportunistic pathogen can lead to invasive candidemia infection. In clinical practice, systemic antifungal therapy and the removal of the potentially infected central venous catheter might be recommended for the treatment of C. lipolytica candidemia.
- Published
- 2013
25. Overexpression of the adeB gene in clinical isolates of tigecycline-nonsusceptible Acinetobacter baumannii without insertion mutations in adeRS
- Author
-
Ming-Chin Chan, Wei-Yao Wang, Tein-Yao Chang, Jun-Ren Sun, and Tzong-Shi Chiueh
- Subjects
Acinetobacter baumannii ,Male ,Molecular Sequence Data ,Minocycline ,Tigecycline ,Drug resistance ,Microbiology ,Bacterial Proteins ,Mechanisms of Resistance ,Drug Resistance, Bacterial ,medicine ,Humans ,Pharmacology (medical) ,Insertion ,Gene ,Antibacterial agent ,Aged ,Pharmacology ,Genetics ,Aged, 80 and over ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Membrane Transport Proteins ,Middle Aged ,biology.organism_classification ,Mutagenesis, Insertional ,Infectious Diseases ,Carbapenems ,Neisseriaceae ,Female ,medicine.drug ,Acinetobacter Infections - Abstract
Thirteen clinical isolates of m ultidrug- r esistant Acinetobacter baumannii r esistant to c arbapenems (MRAB-C) with tigecycline nonsusceptibility were collected from individual patients in this study. None of the 13 isolates shared the same strain characteristics in molecular typing. All of them showed increased adeB transcription, as predicted. However, none of these tigecycline-nonsusceptible MRAB-C isolates were found to possess previously known adeRS mutations. Upregulation of adeB transcription may result from cross stimulation by other mechanisms.
- Published
- 2010
26. Effectiveness analysis of cross-functional team to implement central venous catheter care bundle
- Author
-
Chih-Chien Wang, Tzu-Feng Huang, Yu-Hui Chiu, Ming-Chin Chan, and Ching-Mei Chang
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,General Immunology and Microbiology ,business.industry ,Incidence (epidemiology) ,medicine.medical_treatment ,General Medicine ,Audit ,Checklist ,Infectious Diseases ,Catheterization procedure ,Immunology and Microbiology(all) ,Bundle ,Health care ,Emergency medicine ,medicine ,Immunology and Allergy ,business ,Catheter Site ,Central venous catheter - Abstract
Purpose: Central venous catheterization has become the most important treatment in modern clinical medicine; however, the incidence of central line-associated bloodstream infection (CLABSI) might increase if strict sterile precautions were not followed. Hence, many countries are implementing central venous catheter (CVC) care bundle, which includes hand hygiene, maximal barrier precaution, chlorhexidine skin antisepsis, optimal catheter site selection avoiding femoral site, bundle checklist to supervise catheterization procedure, daily review of line necessity with prompt removal of unnecessary lines. These bundle cares were proved to be effective to significantly reduce the CLABSI incidence. Methods: The study described the processes and results of implementing the CVC care bundle from March 2013 to May 2014 in an 1800 beds medical center in northern Taiwan. The CLABSI was collected and compared between preintervention phase (March 2012eFebruary 2013) and post-intervention phase (March 2013eMay 2014). We organized a special cross-functional team chaired by associate superintendent of the hospital and developed the CVC cart. We also held CVC care bundle educations for all healthcare workers. Audit teams were set and compliance of bundle elements were monitored. Results: After using CVC care bundle elements and equipment, combined with monthly review and weekly audit of the healthcare workers’ compliance, the daily assessment compliance rate rose from 60% to 95% in doctor and maintained 95% and above in nurse. The number of CLABSI dropped from 172 to 137, and the CLABSI incidence rate decreased from 11.47& to 6.88& (p < 0.001). By the result, we can predict that 192 cases were reduced, and about NT$14 million was saved. Conclusions: ImplementingCVCcarebundle isan importantandeffectivemedical practice for patients’ safety. It also significantly reduces the incidence of CLABSI. Therefore, the CVC care bundle is indeed feasible and effective.
- Published
- 2015
27. Investigation of an influenza B outbreak in a nursing home
- Author
-
Ning-Chi Wang, Feng-Yee Chang, Ming-Chin Chan, Ching-Mei Chang, and L.O. Yu-Ning
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Infectious Diseases ,General Immunology and Microbiology ,business.industry ,Immunology and Microbiology(all) ,Family medicine ,medicine ,Immunology and Allergy ,Outbreak ,General Medicine ,Nursing homes ,business - Published
- 2015
28. Needlestick and safety needle promotion effect analysis in a medical center
- Author
-
Yu-Hui Chiu, Ming-Chin Chan, Ning-Chi Wang, and Feng-Yee Chang
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,General Immunology and Microbiology ,business.industry ,Promotion effect ,food and beverages ,General Medicine ,Infectious Diseases ,Immunology and Microbiology(all) ,Family medicine ,Immunology and Allergy ,Medicine ,Center (algebra and category theory) ,business - Published
- 2015
29. Investigation of vancomycin-resistant Enterococci outbreak in an adult intensive care unit of a medical center
- Author
-
Ning-Chi Wang, Cherng-Lih Perng, Ming-Chin Chan, and Tzu-Feng Huang
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,General Immunology and Microbiology ,business.industry ,Outbreak ,Vancomycin-Resistant Enterococci ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,Infectious Diseases ,Adult intensive care unit ,Immunology and Microbiology(all) ,Emergency medicine ,medicine ,Immunology and Allergy ,Center (algebra and category theory) ,business - Published
- 2015
30. A Truncated AdeS Kinase Protein Generated by ISAba1 Insertion Correlates with Tigecycline Resistance in Acinetobacter baumannii
- Author
-
Jung-Chung Lin, Jun-Ren Sun, Chih-Mao Su, Ming-Chin Chan, Tein-Yao Chang, Wei-Yao Wang, Tzong-Shi Chiueh, Yuji Morita, and Cherng-Lih Perng
- Subjects
Acinetobacter baumannii ,Bacterial Diseases ,Transcription, Genetic ,Operon ,lcsh:Medicine ,Minocycline ,Tigecycline ,Drug resistance ,Gene Order ,Molecular Cell Biology ,Pathology ,Cluster Analysis ,Insertion sequence ,lcsh:Science ,Promoter Regions, Genetic ,Multidisciplinary ,Protein Kinase Signaling Cascade ,biology ,Signaling Cascades ,Clinical Laboratory Sciences ,Anti-Bacterial Agents ,Infectious Diseases ,Medicine ,Efflux ,Research Article ,Signal Transduction ,medicine.drug ,Drugs and Devices ,Genotype ,Molecular Sequence Data ,Microbial Sensitivity Tests ,Frameshift mutation ,Microbiology ,Antibiotic resistance ,Diagnostic Medicine ,Drug Resistance, Bacterial ,medicine ,Humans ,Amino Acid Sequence ,Biology ,Base Sequence ,lcsh:R ,Gene Expression Regulation, Bacterial ,biology.organism_classification ,Molecular biology ,Mutagenesis, Insertional ,Mutation ,DNA Transposable Elements ,lcsh:Q ,ATP-Binding Cassette Transporters ,Infectious Disease Modeling - Abstract
Over-expression of AdeABC efflux pump stimulated continuously by the mutated AdeRS two component system has been found to result in antimicrobial resistance, even tigecycline (TGC) resistance, in multidrug-resistant Acinetobacter baumannii (MRAB). Although the insertion sequence, ISAba1, contributes to one of the AdeRS mutations, the detail mechanism remains unclear. In the present study we collected 130 TGC-resistant isolates from 317 carbapenem resistant MRAB (MRAB-C) isolates, and 38 of them were characterized with ISAba1 insertion in the adeS gene. The relationship between the expression of AdeABC efflux pump and TGC resistant was verified indirectly by successfully reducing TGC resistance with NMP, an efflux pump inhibitor. Further analysis showed that the remaining gene following the ISAba1 insertion was still transcribed to generate a truncated AdeS protein by the Pout promoter on ISAba1 instead of frame shift or pre-termination. Through introducing a series of recombinant adeRS constructs into a adeRS knockout strain, we demonstrated the truncated AdeS protein was constitutively produced and stimulating the expression of AdeABC efflux pump via interaction with AdeR. Our findings suggest a mechanism of antimicrobial resistance induced by an aberrant cytoplasmic sensor derived from an insertion element.
- Published
- 2012
31. NKG2A Down-Regulation by Dasatinib Enhances Natural Killer Cytotoxicity and Accelerates Effective Treatment Responses in Patients With Chronic Myeloid Leukemia
- Author
-
Ming-Chin Chang, Hung-I Cheng, Kate Hsu, Yen-Ning Hsu, Chen-Wei Kao, Yi-Fang Chang, Ken-Hong Lim, and Caleb Gonshen Chen
- Subjects
CML ,BCR-ABL ,major molecular response (MMR) ,deep molecular response (DMR) ,natural killer cell ,NKG2A ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Chronic myeloid leukemia (CML) is a hematological malignancy characterized by the presence of t(9;22) chromosomal translocation that results in BCR-ABL fusion gene. ABL tyrosine kinase inhibitors (TKIs), such as imatinib, nilotinib, and dasatinib, are currently the front-line treatment options for CML. Recently, natural killer (NK) cell activation and expansion have been shown to be associated with optimal treatment responses for CML. To investigate the effects and mechanisms of these TKIs on NK cells, here we characterized activating and inhibitory NK receptors in CD3−CD16+CD56dim NK cells isolated from CML patients in chronic phase (CP). The expressions of activating NK receptors, such as NKG2D, natural cytotoxicity receptor (NCR) and DNAM-1, rebounded after successful TKI treatments for CML. In contrast, among the three surveyed inhibitory receptors (NKG2A, KIR2DL1, and KIR3DL1), only the expression of NKG2A was reverted and suppressed to a very low level by dasatinib, and not by imatinib or nilotinib. CML patients treated with dasatinib indeed expressed fewer NKG2A+ NK cells, which send negative signals for induction of NK cytotoxicity. For these dasatinib-treated patients, the duration to reach major molecular response (MMR) was shorter, and significantly correlated with individual's NKG2A+ NK cell number. This clinical relevance to NKG2A was not observed in treatments with imatinib or nilotinib. In line with dasatinib-specific down-regulation of NKG2A, NK cytotoxicity evaluated by the killing assay was also significantly higher in patients treated with dasatinib than in those treated with imatinib or nilotinib. The lower NK cytotoxicity from imatinib or nilotinib treatments could be reverted by NKG2A blockade using anti-NKG2A antibody. Further in vitro experiments revealed mechanistically that dasatinib could inactivate p38 mitogen-activated protein kinase (MAPK), and consequently affect nuclear import of GATA-3 and GATA-3 transcriptional activities for NKG2A. Our results highlight the dual effects of dasatinib in direct inhibition of ABL kinase and in immunomodulation through NKG2A down-regulation, contributing to accelerated molecular responses (MR) in CML.
- Published
- 2019
- Full Text
- View/download PDF
32. An investigation of vancomycin minimum inhibitory concentration creep among methicillin-resistant Staphylococcus aureus strains isolated from pediatric patients and healthy children in Northern Taiwan
- Author
-
Ching-Mei Yu, Chia-Ning Chang, Ming-Chin Chan, Chih-Chien Wang, and Wen-Tsung Lo
- Subjects
0301 basic medicine ,Male ,Meticillin ,lcsh:QR1-502 ,medicine.disease_cause ,lcsh:Microbiology ,Tertiary Care Centers ,Immunology and Allergy ,Child ,Vancomycin creep ,General Medicine ,Staphylococcal Infections ,Anti-Bacterial Agents ,Electrophoresis, Gel, Pulsed-Field ,Infectious Diseases ,Staphylococcus aureus ,Child, Preschool ,Carrier State ,Vancomycin ,Female ,Methicillin-resistant Staphylococcus aureus ,medicine.drug ,Microbiology (medical) ,Adolescent ,Genotype ,030106 microbiology ,Taiwan ,Microbial Sensitivity Tests ,Microbiology ,03 medical and health sciences ,Minimum inhibitory concentration ,Daptomycin ,Immunology and Microbiology(all) ,medicine ,Pulsed-field gel electrophoresis ,Humans ,Etest ,Retrospective Studies ,General Immunology and Microbiology ,business.industry ,Infant, Newborn ,Genetic Variation ,Infant ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Molecular Typing ,Pulsed-field gel electrophoresis type of MRSA ,business - Abstract
Background and purpose: The phenomenon of vancomycin minimum inhibitory concentration (MIC) creep is an increasingly serious problem in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. In this study, we investigated the vancomycin and daptomycin MIC values of MRSA strains isolated from pediatric patients and MRSA colonized healthy children. Then, we assessed whether there was evidence of clonal dissemination for strains with an MIC to vancomycin of ≥ 1.5 μg/mL. Methods: We collected clinical MRSA isolates from pediatric patients and from healthy children colonized with MRSA during 2008–2012 at a tertiary medical center in northern Taiwan and obtained vancomycin and daptomycin MIC values using the Etest method. Pulse-field gel electrophoresis (PFGE) and staphylococcal cassette chromosome (SCCmec) typing were used to assess clonal dissemination for strains with an MIC to vancomycin of ≥ 1.5 μg/mL. Results: A total 195 MRSA strains were included in this study; 87 were isolated patients with a clinical MRSA infection, and the other 108 strains from nasally colonized healthy children. Vancomycin MIC≥1.5 μg/mL was seen in more clinical isolates (60/87, 69%) than colonized isolates (32/108, 29.6%), p
- Full Text
- View/download PDF
33. Solar and Visible Light Illumination on Immobilized Nano Zinc Oxide for the Degradation and Mineralization of Orange G in Wastewater
- Author
-
Hung-Yee Shu, Ming-Chin Chang, and Tien-Hsin Tseng
- Subjects
ZnO ,visible light ,photocatalytic degradation ,orange G ,immobilization ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
An advanced oxidation process (AOP) utilizing immobilized zinc oxide (ZnO) photocatalyst was employed to decolorize and mineralize orange G (OG) azo dye in wastewater under solar and visible light irradiation. This AOP employed visible light and ZnO in a so-called Vis/ZnO process. Operating parameters, including ZnO dosage, initial OG concentration, pH, visible-light intensity, catalyst loaded area, and treatment volume were investigated to illustrate their influences on OG degradation and mineralization. From the results, neither visible light alone, nor the ZnO adsorption process could degrade or remove OG from wastewater. However, for the Vis/ZnO process, the higher ZnO dosage and visible light intensity are two major parameters to improve the OG degradation and total organic carbons (TOC) mineralization. The initial pH of 11 was the most effective pH condition on the OG degradation. The first-order rate constant is exponentially decreased from 0.025 to 0.0042 min−1 with the increase of the initial OG concentration and an empirical equation can be derived to estimate the first-order rate constant with a known initial OG concentration. In contrast, the first-order rate constant is linearly increased from 0.0027 to 0.0083 min−1 by increasing the visible light intensity. The results present that the Vis/ZnO process is an effective AOP for the degradation of OG in wastewater.
- Published
- 2017
- Full Text
- View/download PDF
34. Evaluation of using disposable wipes and real time adenosine triphosphate bioluminescence monitoring: A new intensive care unit cleaning program
- Author
-
Yu-Hui Chiu, Jung-Chung Lin, Ching-Mei Chang, Te-Yu Lin, Tzu-Feng Huang, Tzu-Ling Liu, Ming-Chin Chan, Pei-Shan Hung, and Sheng-Kang Chiu
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,General Immunology and Microbiology ,business.industry ,General Medicine ,Intensive care unit ,law.invention ,chemistry.chemical_compound ,Infectious Diseases ,chemistry ,law ,Immunology and Microbiology(all) ,medicine ,Immunology and Allergy ,Bioluminescence ,Intensive care medicine ,business ,Adenosine triphosphate - Full Text
- View/download PDF
35. Investigation of a Klebsiella pneumoniae outbreak in hematology and oncology ward of a medical center
- Author
-
Ming-Chin Chan, Sung-Teng Hsu, Ning-Chi Wang, Ching-Mei Chang, and Feng-Yee Chang
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Hematology ,General Immunology and Microbiology ,biology ,business.industry ,Klebsiella pneumoniae ,Outbreak ,virus diseases ,General Medicine ,biology.organism_classification ,Infectious Diseases ,Internal medicine ,Immunology and Microbiology(all) ,medicine ,Immunology and Allergy ,Center (algebra and category theory) ,business ,Intensive care medicine - Full Text
- View/download PDF
36. Experiences of implementing UTI bundle care in an emergency intensive care unit of a medical center
- Author
-
Feng-Yee Chang, Ning-Chi Wang, Ming-Chin Chan, and Mei-Chuan Tsai
- Subjects
Microbiology (medical) ,General Immunology and Microbiology ,business.industry ,General Medicine ,Intensive care unit ,law.invention ,Infectious Diseases ,Ambulatory care ,Nursing ,law ,Immunology and Microbiology(all) ,Bundle ,Critical care nursing ,Immunology and Allergy ,Medicine ,Center (algebra and category theory) ,business - Full Text
- View/download PDF
37. Supported Zinc Oxide Photocatalyst for Decolorization and Mineralization of Orange G Dye Wastewater under UV365 Irradiation
- Author
-
Ming-Chin Chang, Hung-Yee Shu, Tien-Hsin Tseng, and Hsin-Wen Hsu
- Subjects
Renewable energy sources ,TJ807-830 - Abstract
To solve the environmental challenge of textile wastewater, a UV/ZnO photocatalytic system was proposed. The objective of this study was to prepare a photocatalytic system by utilizing both cold cathode fluorescent light (CCFL) UV irradiation and steel mesh supported ZnO nanoparticles in a closed reactor for the degradation of azo dye C.I. Orange G (OG). Various operating parameters such as reaction time, preparation temperature, mixing speed, ZnO dosage, UV intensity, pH, initial dye concentration, and service duration were studied. Results presented efficient color and total organic carbon (TOC) removal of the OG azo dye by the designed photocatalytic system. The optimal ZnO dosage for color removal was 60 g m−2. An alkaline pH of 11.0 was sufficient for photocatalytic decolorization and mineralization. The rate of color removal decreased with the increase in the initial dye concentration. However, the rate of color removal increased with the increase in the UV intensity. The steel mesh supported ZnO can be used repeatedly over 10 times without losing the color removal efficiency for 120 min reaction time. Results of Fourier transform infrared (FTIR) and ion chromatography (IC) indicated the breakage of N=N bonds and formation of sulfate, nitrate, and nitrite as the major and minor products. The observation indicated degradation of dye molecules.
- Published
- 2013
- Full Text
- View/download PDF
38. A New Photocatalytic System Using Steel Mesh and Cold Cathode Fluorescent Light for the Decolorization of Azo Dye Orange G
- Author
-
Ming-Chin Chang, Chin-Pao Huang, Hung-Yee Shu, and Yung-Chen Chang
- Subjects
Renewable energy sources ,TJ807-830 - Abstract
High color and organic composition, the effluents from the textile dyeing and finishing industry, can be treated by photocatalytic oxidation with UV/TiO2. The objective of this study was to prepare a new photocatalytic system by coating nanosized TiO2 particles on steel mesh support and using cold cathode fluorescent light (CCFL) irradiation at 365 nm in a closed reactor for the oxidation of azo dye C.I. Orange G (OG). Various factors such as reaction time, coating temperature, TiO2 dosage, pH, initial dye concentration, and service duration were studied. Results showed efficient color removal of the OG azo dye by the photocatalytic system with TiO2-coated temperature at 150°C. The optimal TiO2 dosage for color removal was 60 g m−2. An acidic pH of 2.0 was sufficient for photocatalytic oxidation whereas basic condition was not. The rate of color removal decreased with increase in the initial dye concentration. The TiO2-coated steel mesh can be used repeatedly over 10 times without losing the photocatalytic efficiency. Results of FTIR and IC indicated the breakage of N=N bonds, with sulfate as the major and nitrite and nitrate as the minor products, which implied degradation of dye molecules.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.