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Previous research has demonstrated negative associations between task-relevant conflicts and collaboration. To supplement the previous findings and explore the potential benefits of conflicts, we differentiate between two types of task conflict expressions (i.e., mild vs. intense task conflicts, such as debates vs. disagreements regarding work-related issues) in dyad interactions and propose the differential effects of mild versus intense task conflicts on collaboration based on the theory of conflict expression. In three studies with experimental manipulations and surveys on working adults, the results demonstrated that perceptions of debates versus disagreements (in Studies 1 and 2) or mild versus intense task conflicts (in Study 3) enhanced perceivers' collaboration with others via the perceivers' assessments of others' openness rather than emotions. The findings regarding positive associations between mild task conflicts and collaboration implicate the coexistence of conflict and collaboration. Moreover, the results showed that debates versus disagreements (in Study 1) or mild versus intense task conflicts (in Study 3) achieved high task performance by enhancing perceptions of others' openness that subsequently increased collaboration. These findings clarify why conflicts inconsistently influence interpersonal interactions and task performance. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Protocadherin 10 (PCDH10) is identified as a tumor suppressor in multiple cancers. The molecular mechanisms that mediate the functions of PCDH10 have yet to be fully elucidated. Here, we demonstrated that ectopic expression of PCDH10 in colorectal cancer (CRC) cells induced cell cycle retardation and increased apoptosis through regulation of the p53/p21/Rb axis and Bcl-2 expression. Overexpression of PCDH10 reversed the epithelial-mesenchymal transition (EMT) process with morphological changes and EMT marker alterations. Mechanistic study revealed that PCDH10 inhibited AKT/GSK3β signaling pathway which in turn reduced β-catenin activity and thus attenuated Snail and Twist1 expression. Furthermore, PCDH10 inhibited the stemness of CRC cells, including spheroid formation and stem cell markers. A proteomics approach revealed that PCDH10 could interact with EGFR, which was further verified by co-immunoprecipitation. Moreover, restoration of PCDH10 expression reduced EGFR phosphorylation. Accordingly, our work proposes a novel pathway by which PCDH10 directly engages in the negative regulation of EGFR/AKT/β-catenin signaling pathway, resulting in tumor suppression.

This research developed an adaptive control system for injection molding process. The purpose of this control system is to adaptively maintain the consistency of product quality by minimize the mass variation of injection molded parts. The adaptive control system works with the information collected through two sensors installed in the machine only—the injection nozzle pressure sensor and the temperature sensor. In this research, preliminary experiments are purposed to find master pressure curve that relates to product quality. Viscosity index, peak pressure, and timing of the peak pressure are used to characterize the pressure curve. The correlation between product quality and parameters such as switchover position and injection speed were used to produce a training data for back propagation neural network (BPNN) to compute weight and bias which are applied on the adaptive control system. By using this system, the variation of part weight is maintained to be as low as 0.14%.

The quality of stem cells obtained through serial subcultivation is the pivotal factor determining the therapeutic effectiveness of regenerative medicine. However, an effective quality monitoring system for cell culture is yet to be established. Detailed parameter studies of the migratory behavior of stem cells at different passages may provide insight into the deterioration of stemness. Thus, this study aimed to evaluate the feasibility of quantitative bioimage analysis for monitoring stem cell quality during in vitro culture and to explore the passaging effects on stem cell migration. An image-based analytical tool using cell tracking, cytometric analyses, and gating with time-lapse microscopy was developed to characterize the migratory behavior of human mesenchymal stem cells (hMSCs) isolated from human adipose tissue (hADAS) and placenta (hPDMC) cultured on chitosan membranes. Quantitative analysis was performed for the single cells and assembled spheroids selected from 15 videos of Passages 3, 5, and 11 for hADAS and those from 12 videos of Passages 7, 11, and 16 for hPDMC. These passages were selected to represent the young, matured, and degenerated stem cells, respectively. Migratory behavior varied with cell passages. The mobility of single hMSCs decreased at degenerated passages. In addition, enhancement of mobility, due to transformation from single cells to spheroids, occurred at each passage. The young hMSCs seemed more likely to move as single cells rather than as aggregates. Once matured, they tended to aggregate with strong 3D spheroid formability and increased mobility. However, the spheroid formability and mobility decreased at late passage. The increase in aggregation rate with passaging may be a compensatory mechanism to enhance the declining mobility of hMSCs through cell coordination. Our findings regarding the passaging effects on stem-cell migratory behavior agree with biochemical reports, suggesting that the developed imaging method is capable of monitoring the cell-culture quality effectively. © 2020 International Society for Advancement of Cytometry.

People seek warm and trustworthy individuals as long-term mates for numerous reasons. Indeed, such individuals are prone to cooperation, have strong parenting skills, have the ability to fulfill our need to belong, and may provide a relationship that is characterized by greater closeness, protection, acceptance, and safety. Although prior work has shown that both sexes indicate equally strong preferences for these traits in potential mates, few studies have examined whether people actually respond favorably to partners high in warmth-trustworthiness in live mating contexts. We, thus, demonstrated that people’s stated preferences for warmth-trustworthiness (a) predicted their attraction to potential mates in a live mate-selection context (Study 1) and (b) interacted with their partners’ actual traits to predict satisfaction with their marriages (Study 2). Together, these studies demonstrate the importance of partner traits associated with warmth and trustworthiness and add to recent research suggesting that people can accurately report their romantic-partner preferences.

When interacting with a task partner, individuals often rely upon characteristics they infer about their partner to determine their level of collaboration with the partner. To explore social perception processes related to collaboration, we examined perceptions of an actor’s decision-making style as predictors. Using different methods in various research settings, we found that compared with perceptions of an actor’s intuitive decision-making style or of a nonspecific decision-making style, perceptions of an actor’s rational decision-making style were more positively associated with inferences of the actor’s openness and competence, both of which were in turn associated with the perceiver’s collaborative intention with the actor. Intentions to engage in mutual collaboration were also positively associated with performance in an idea generation task. Therefore, our research offers a novel illustration of how to enhance collaboration based on perceptions of openness and competence inferred from others’ rational rather than intuitive decision-making style.

To increase the productivity of injection molding machines, we developed a smart injection part weight stability control system based on C++ programming and domain knowledge. The proposed system is meant to eliminate variability in the quality of injected parts by adjusting the changeover position. We developed a viscosity index based on melt pressure data related to guide the adjustment to the changeover position in accordance with material properties. This was achieved by mounting a pressure sensor on the nozzle of the injection molding machine to enable the on-line monitoring of pressure throughout the injection molding process. A series of experiments was conducted to characterize the relationship between viscosity index and injection-molded samples in order to validate the efficacy of the proposed injection stability system. Single-factor experiments were conducted with the changeover position and melt temperature as parameters. The quality of the molded samples obtained under different process parameters was evaluated in terms of weight. Experiment results revealed a correlation between changes in viscosity index and changes in the weight of the samples. The injection stability system can also be operated in self-adjusting mode, in which the changeover position is varied according to viscosity index. In experiments, abnormal machine operations prompted the adjustment of changeover position. Variation in the weight of parts was used to define an index to validate the efficacy of the proposed system.

Objective We investigated whether men's social confidence in an initial, opposite‐sex chatting context can be improved through a video tutorial and the extent to which being perceived as socially confident results in being seen as more romantically desirable and worthy of future contact. Method Women chatted with men who had received or not received a tutorial on how to handle speed‐dating chats (Study 1: N = 129; Study 2: N = 60) or with male targets selected for having high versus moderate confidence in handling initial, opposite‐sex encounters (Study 3: N = 46). Results Tutorial‐trained men felt more confident going into the chats and they, as well as male targets selected for their confidence, were perceived by female chat partners to be higher in social confidence, status, and dominance. However, only perceptions of social confidence were further associated with being perceived as more romantically desirable (as a short‐term mate) and worthy of future contact. Conclusions Findings indicate that social confidence is trainable and that other‐perceived social confidence can impact the outcomes of social interactions.

This year’s academy theme “Bringing the Manager Back in Management” focuses on what managers do on a day-to-day basis. This panel symposium is designed to engage a group of scholars and practitione...

We investigated a potential outcome of ego depletion manipulations and an important factor behind cooperative failure: a lack of openness to others' dissenting opinions. Across five studies in a variety of task settings, we examined the effect of depletion manipulations on openness to dissent and investigated two negative emotions as potential mediators of this process: fatigue and anger. The results demonstrated a negative effect of depletion manipulations on openness to dissent through increased anger rather than fatigue (Studies 1-5). In Studies 3 and 4, we also eliminated perceived trust towards a task counterpart as a significant mediator of the relationship between depletion manipulations and openness to dissent. These findings help clarify the nature of ego depletion manipulations and shed light on why individuals may fail to consider others' dissenting opinions and, thus, fall short of achieving cooperation.

Shisa3 is a novel tumor suppressor identified in lung cancer. However, its antitumor activity in other human cancers and the mechanism of gene inactivation remain unknown. SHISA3 expression was measured by reverse transcription-PCR (RT-PCR) and quantitative RT-PCR (RT-qPCR). DNA methylation was determined by bisulfite sequencing and pyrosequencing. Down-regulation of SHISA3 expression was observed in all of 11 colorectal cancer (CRC) cell lines and was further confirmed in 34 (65.4 %) of 52 colorectal carcinomas by RT-qPCR. Four of six CRC cell lines could restore SHISA3 expression after treatment with 5-aza-2′-deoxycytidine. Tumor-specific methylation of five CpG sites in the first intron of SHISA3 was identified by bisulfite sequencing, and their methylation levels were quantified in 127 pairs of primary CRC tissues by bisulfite pyrosequencing. The methylation levels of SHISA3 in tumors were noticeably higher than that in their matched normal mucosae. In addition, SHISA3 hypermethylation was significantly associated with an increased risk of disease recurrence in patients with stage II and III disease (P = 0.007) and was an independent predictor of poor overall survival [hazard ratio (HR) 2.9, 95 % confidence interval (CI) 1.5–5.8; P = 0.002] and disease-free survival (HR 4.0, 95 % CI 1.6–10.2; P = 0.003) of CRC patients. SHISA3 gene is epigenetically inactivated in a substantial fraction of CRC, and its hypermethylation is of prognostic significance in predicting clinical outcome. The quantitative bisulfite pyrosequencing assay established could be a cost-effective tool for providing a potential biomarker of adverse prognosis in CRC.

Protocadherin 10 (PCDH10), a novel tumor suppressor gene in human cancers, is located in a common deleted region at chromosome 4q28 in colorectal cancer (CRC). This study aimed to ascertain the genetic loss of PCDH10 and its clinical relevance in CRC and to explore the tumor suppressor function of PCDH10. The genetic deletion of PCDH10 was determined in 171 pairs of primary tumors and corresponding normal mucosae by loss of heterozygosity study. In total, 53 carcinomas were positive for allelic loss of PCDH10. The genetic aberration was significantly associated with tumor progression and distant metastasis (p = 0.021 and p = 0.018, respectively) and was an independent predictor of poor survival for CRC patients (p = 0.005). Expression of PCDH10 gene was silenced or markedly down-regulated in all of 12 CRC cell lines tested and in 41 of 53 colorectal carcinomas compared with their matched normal mucosae. Ectopic expression of PCDH10 suppressed cancer cell proliferation, anchorage-independent growth, migration and invasion in vitro. Subcutaneous injection of PCDH10-expressing CRC cells into SCID mice revealed the reduction of tumor growth compared with that observed in mock-inoculated mice. Furthermore, through intrasplenic implantation, the re-expression of PCDH10 in silenced cells restrained liver metastasis and improved survival in SCID mice. In conclusion, PCDH10 is a pivotal tumor suppressor in CRC, and the loss of its function promotes not only tumor progression but also liver metastasis. In addition, the genetic deletion of PCDH10 represents an adverse prognostic marker for the survival of patients with CRC.

// Tzu-Ming Jao 1 , Ya-Lin Li 1 , Shu-Wha Lin 1, 2 , Sheng-Tai Tzeng 1 , I-Shing Yu 3 , Sou-Jhy Yen 4 , Ming-Hong Tsai 4, 5 , Ya-Chien Yang 1, 2 1 Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan 2 Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan 3 Laboratory Animal Center, National Taiwan University College of Medicine, Taipei, Taiwan 4 Department of Surgery, Cardinal Tien Hospital, New Taipei City, Taiwan 5 School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan Correspondence to: Ming-Hong Tsai, email: tsai110008@yahoo.com.tw Ya-Chien Yang, email: ycyangntu@ntu.edu.tw Keywords: NDST4, tumor suppressor gene, heparan sulfate proteoglycan, Ndst4 knockout mouse Received: July 29, 2016 Accepted: October 14, 2016 Published: October 26, 2016 ABSTRACT Glucosaminyl N -deacetylase/ N -sulfotransferases (NDSTs) are the first enzymes that mediate the initiation of heparan sulfate sulfation. We previously identified NDST4 as a putative tumor suppressor in human colorectal cancer. In the study, we generated an Ndst4 knockout ( Ndst4 −/− ) mouse strain and explored its phenotypic characteristics, particularly in the development of colonic epithelial homeostasis. The Ndst4-deficient mice were viable and fertile, and their life spans were similar to those of wild-type littermates. No gross behavioral or morphological differences were observed between the Ndst4 −/− and wild-type mice, and no significant changes were determined in the hematological or serum biochemical parameters of the Ndst4 −/− mice. Ndst4 RNA transcripts were expressed in the brain, lung, gastrointestinal tract, pancreas, and ovary. However, Ndst4-null mice exhibited no gross or histological abnormalities in the studied organs, except for the colon. Although no alterations were observed in the crypt length or number of proliferating cells, the Ndst4 −/− mice exhibited an increased number of goblet cells and a decreased number of colonocytes in the proximal colon compared with the wild-type mice. Moreover, Ndst4 deficiency increased the basal level of apoptosis in the colonic epithelium. Taken together, we established, for the first time, an Ndst4 −/− mouse strain and revealed the involvement of Ndst4 in the development and homeostasis of colonic epithelium. Accordingly, NDST4 in human colon might direct the biosynthesis of specific heparan sulfate proteoglycans that are essential for the maintenance of colonic epithelial homeostasis. Thus, the loss of its function may result in the tumorigenesis and progression of colorectal cancer.

Two studies examined how discrete emotions influence escalation of commitment. Study 1 demonstrated that anger was associated with more escalation of commitment than fear in a personnel hiring-appraisal context. In addition, it revealed the mediating effect of risk perception; angry compared to fearful individuals perceived lower risk in their initial decision, which in turn increased the tendency to escalate commitment. Study 2 replicated the pattern of results of Study 1 in a financial decision-making situation. Contrary to conclusions drawn from the results of prior research, the current studies suggest that not all negative emotions alleviate escalation of commitment.

In order to improve the field emission properties of the graphite flakes, the carbon nanotubes (CNTs) are produced on above without the metallic catalyst using mixtures of C2H2 and H2 gases by thermal chemical vapor deposition. We spin the graphite solution on the silicon wafer and dry it, then synthesize the CNTs on the graphite flakes. We change the synthetic time to obtain the optimal conditions for enhancement of field emission properties of graphite flakes. The experimental results show that the density and quality of the CNTs could be controlled significantly by the synthetic time. Besides, the field emission properties of the treated graphite flakes are also affected greatly by it. The emission current density of the treated graphite flakes reaches to 0.5 mA/cm2 at 3 V/μm, and the turn-on field is decreased from 7.7 to 1.9 V/μm after producing the CNTs on above.

In order to improve the field electron emission properties of the graphite, the carbon nanoparticles (CNPs) are synthesized on the micrographite flakes by hydrogen thermal processing. We spin the graphite solution on the silicon wafer and desiccate it, then produce the CNPs on the graphite flakes using hydrogen thermal processing in the furnace. The processing parameters such as the processing temperature, hydrogen flow rate and processing time, were varied to find the optimal conditions for the improvement of the field emission properties of the graphite flakes. The experimental results show that the field emission properties of the graphite flakes have glaring improvement after heat treatment owing to the increase of the defect density and the CNPs on above. The turn-on field was decreased from 7.7 of the untreated sample to 4.3 V/μm of the treated sample at the optimal processing conditions.

This study examines individual and cultural antecedents of incivility in the workplace, using a sample of MBAs and EMBAs from Taiwan and the United States. We predicted that individual achievement orientation would enhance incivility, based on Dollard’s frustration aggression hypothesis, and that those who were higher in direct conflict self-efficacy (i.e., beliefs in one's skills in managing direct conflict) would be higher in incivility. These predictions were supported. We also predicted, and found, that collectivism orientation constrains these main effects, so that for those high in collectivism, the impact of achievement orientation and direct conflict self-efficacy is weak or nonexistent. Implications for conflict management are discussed.

The present study examined bystanders' justice perceptions about co-punishment events. In a sample of 169 logistic officers in the Taiwanese military, responsibility attributions (i.e. liability attributed to co-punished persons) had a negative relationship with perceived harshness, and a positive relationship with perceived procedural justice. In addition, the effects of responsibility attributions on procedural justice were weaker if the person perceived stronger rather than weaker organizational norms of co-punishment.

This study samples 78 business decision‐makers whose cases were part of an alternative dispute resolution (ADR) process, i.e., the Public Construction Commission (PCC), which operates under the government in Taiwan, between 1997 and early 2000. The authors propose an interaction between two variations of trust—category‐based trust and experience‐based trust—and hypothesize that decision‐makers’ perceived identity with new versus old government ideology and past justice experiences (with the PCC) would jointly affect their decision preferences. The results partially support these hypotheses. The authors emphasize the critic role of trustworthiness of the third‐party ADR providers. We conclude with a discussion of the implications of the findings.

We developed new materials to induce a luxury mindset and activate materialistic values, and examined materialism’s relationship to attitudes toward marriage and having children in Singapore. Path analyses indicated that materialistic values led to more negative attitudes toward marriage, which led to more negative attitudes toward children, which in turn led to a decreased number of children desired. Results across two studies highlight, at the individual level, the tradeoff between materialistic values and attitudes toward marriage and procreation and suggest that a consideration of psychological variables such as materialistic values may allow for a better understanding of larger-scale socioeconomic issues including low fertility rates among developed countries. We discuss implications and describe how psychological factors relating to low fertility fit within evolutionary mismatch and life history theory frameworks.

This paper studies the wall effect on drag forces in molding flow using optical fiber sensing data. The results indicate that the effect of the wall cavity is significant in the calculation of the drag forces and is required to be considered in molding. The study also shows the effectiveness of the approach in characterizing the behavior of the wire sweep in the encapsulation of semiconductor chips. An in-line fiber etalon (ILFE) sensor is designed and fused in the middle of the optical fiber for accurate strain measurement. This is done by first laying an optical fiber in the mid-plane of a simple rectangular mold cavity, and then measuring the strain at the mid-span of the optical fiber that is subjected to the flow of homogeneous fluid. For a given flow field, several drag force models have been used to calculate the drag forces over the optical fiber. The resulting strain of the optical fiber is estimated analytically first by FEM and then compared with experimental results. To study the wall effect, different sizes of mold cavities are employed for different b/a ratios and used in Takaisi’s model. Subsequently, Takaisi’s model is then modified to comply with the experimental data obtained by using various b/a ratios for comparison with the numerical drag force model computed by Han and Wang. The result indicates that a modified Takaisi model in compliance with the requirement of b/a within the range 20–200 is in good agreement with the numerical model proposed by Han and Wang who had taken effect of the wall cavity into consideration for drag-force calculation. The modified model based on a smaller b/a value is somewhat closer to the numerical model in a higher average cavity velocity. The wall effect of molding flow is concluded to be an important factor for various tests conducted at a greater average cavity velocity or a smaller value of b/a .

The signals between cascaded Fabry–Perot sensors have serious cross-talk problems due to complex phase interactions between sensors. However, cross-talks in cascaded Fabry–Perot sensors for demodulation in a path-matching differential interferometry (PMDI) can be limited by corresponding path-matching read-out systems. This paper presents a method of using spectrum analysis to design the series Fabry–Perot sensors without cross-talk. The spectrum transfer function in a light source and Fabry–Perot cavities are simulated by a Gaussian distribution spectrum and a low-finesse reflectivity, respectively. It shows that two series Fabry–Perot sensors without cross-talk can be designed precisely and following the same procedure, even more series Fabry–Perot sensors without cross-talks in PMDI can be achieved. In-line fiber etalon (ILFE) as a Fabry–Perot cavity is used for multiplexing in the experiment. Experimental data shows that the cross-talk between two series Fabry–Perot sensors is limited. This design in two series Fabry–Perot sensors in PMDI can be applied to a two-parameter optical fiber sensors.

This paper outlines a five-year work of developing a distribution automation laboratory for undergraduate and graduate education at the Electrical Engineering Department of National Taiwan University. A scaled-down three phase distribution system model with a data acquisition system connected to a personal computer is built to facilitate physical experiments and real-time measurements. Remote control of the switches in the distribution system is made possible through the function of 8255 output ports and solid-state relays. Digital simulation programs are also written in order for the students to understand the basic concepts before they actually perform experiments. Three important applications in distribution automation, fault location identification, feeder reconfiguration, and feeder reactive power/voltage control using capacitor switching, are described.

Protocadherin 10 (PCDH10), a novel tumor suppressor gene in human cancers, is located in a common deleted region at chromosome 4q28 in colorectal cancer (CRC). This study aimed to ascertain the genetic loss of PCDH10 and its clinical relevance in CRC and to explore the tumor suppressor function of PCDH10. The genetic deletion of PCDH10 was determined in 171 pairs of primary tumors and corresponding normal mucosae by loss of heterozygosity study. In total, 53 carcinomas were positive for allelic loss of PCDH10. The genetic aberration was significantly associated with tumor progression and distant metastasis (p = 0.021 and p = 0.018, respectively) and was an independent predictor of poor survival for CRC patients (p = 0.005). Expression of PCDH10 gene was silenced or markedly down-regulated in all of 12 CRC cell lines tested and in 41 of 53 colorectal carcinomas compared with their matched normal mucosae. Ectopic expression of PCDH10 suppressed cancer cell proliferation, anchorage-independent growth, migration and invasion in vitro. Subcutaneous injection of PCDH10-expressing CRC cells into SCID mice revealed the reduction of tumor growth compared with that observed in mock-inoculated mice. Furthermore, through intrasplenic implantation, the re-expression of PCDH10 in silenced cells restrained liver metastasis and improved survival in SCID mice. In conclusion, PCDH10 is a pivotal tumor suppressor in CRC, and the loss of its function promotes not only tumor progression but also liver metastasis. In addition, the genetic deletion of PCDH10 represents an adverse prognostic marker for the survival of patients with CRC.

Background Genomic deletion at tumor suppressor loci is a common genetic aberration in human cancers. The study aimed to explore candidate tumor suppressor genes at chromosome 4q25-q28.2 and to delineate novel prognostic biomarkers associated with colorectal cancer (CRC). Methods Deletion mapping of chromosome 4q25-q28.2 was conducted in 114 sporadic CRC by loss of heterozygosity study with 11 microsatellite markers. A novel candidate tumor suppressor gene, namely NDST4, was identified at 4q26. Gene expression of NDST4 was investigated in 52 pairs of primary CRC tissues by quantitative reverse transcription-polymerase chain reaction. Allelic loss of NDST4 gene was further determined in 174 colorectal carcinomas by loss of heterozygosity analysis, and then was assessed for clinical relevance. Results One minimal deletion region was delineated between D4S2297 and D4S2303 loci at 4q26, where NDST4 was the only gene that had markedly been downregulated in CRC tumors. By laser capture microdissection, NDST4 RNA expression was demonstrated in colonic epithelial cells, but was undetectable in tumor cells. In total, 30 (57.7%) of 52 colorectal carcinomas showed a dramatic reduction in NDST4 gene expression compared with matched normal mucosae. The genetic loss of NDST4 was significantly associated with advanced pathological stage (P = 0.039) and poorer overall survival of patients (P = 0.036). Conclusions NDST4 gene is a novel candidate tumor suppressor gene in human cancer, and the loss of its function might be involved in CRC progression. In addition, the loss of heterozygosity assay, which was established to determine the allelic loss of NDST4 gene, could be a cost-effective tool for providing a useful biomarker of adverse prognosis in CRC.

The current research explored the effect of anger on hypothesis confirmation-the propensity to seek information that confirms rather than disconfirms one's opinion. We argued that the moving against action tendency associated with anger leads angry individuals to seek out more disconfirming information than sad individuals, attenuating the confirmation bias. We tested this hypothesis in two studies of experimentally primed anger and sadness on the selective exposure to hypothesis confirming and disconfirming information. In Study 1, participants in the angry condition were more likely to choose disconfirming information than those in the sad or neutral condition when given the opportunity to read more about a social debate, and reading the disconfirming information affected their subsequent attitude. Study 2 measured participants' opinions and information selection about the 2008 US Presidential Election and their desire to "move against" a person or object. Participants in the angry condition reported a greater tendency to oppose a person or object, which resulted in the attenuation of the confirmation bias.

There is no detailed analysis of loss of heterozygosity (LOH) on chromosome 3 in colorectal cancer (CRC). Our aim was to define frequently deleted loci on chromosome 3 and to explore novel prognostic markers and the locations of candidate tumor suppressor genes associated with CRC. LOH at 23 microsatellite markers spanning on chromosome 3 was determined in 112 sporadic CRC by automated fluorescence-based polymerase chain reaction. Genetic loss was assessed for the clinicopathological significance by univariate and multivariate analyses. Fifty-eight (51.8%) of 112 carcinomas exhibited LOH at one or more loci tested. Among seven loci with high LOH rates, allelic losses at D3S1297 and D3S1266 occurred more frequently in younger patients. A marked gender distortion for genetic deletion was observed at six loci, where LOH was identified more frequently in male cases. For clinical outcome, LOH solely at D3S1297 (3p26.3) was significantly associated with distant metastasis (P = 0.001) and was indicative of a shorter overall survival (P = 0.014). In addition, loss of one common deletion region at 3p25-pter was significantly correlated to distant metastasis (P = 0.009) and had an adverse effect on patients’ overall survival in univariate and multivariate tests (P = 0.009 and 0.001, respectively). Loss of chromosome 3p25-pter could act as an independent predicator of poor prognosis in CRC, suggesting that microsatellite analysis is a useful means to stratify patients into different risk groups. In addition, inactivation of candidate tumor suppressor genes in this region might involve in CRC progression.

Groups often have members who hold opposing opinions on specific issues. The presence of undecided people within a group may promote cooperation among group members who hold opposing views on an issue under consideration. The study examined the joint effects of group attitude diversity (i.e. mixed attitude diversity vs. polarized attitude diversity) and one’s strength of attitude on the cooperation. In groups considering a controversial issue with no undecided group members (i.e. polarized attitude diversity), people with strong attitudes were less likely than those with weak attitudes to cooperate with group members who held opposing views. However, the above differences became non-significant when participants were placed in groups with some undecided group members (i.e. mixed attitude diversity). The results from the study suggested that the presence of undecided group members mitigates the negative impact of attitude strength on subsequent cooperation.

Malfunction of mismatch repair (MMR) system and p53 produces nuclear genomic instability and is involved in colorectal tumorigenesis. In addition to a nuclear genome, eukaryotic cells have cytoplasmic genomes that are compartmentalized in the mitochondria. The aims of this study were to detect the mitochondrial genomic instability (mtGI) in colorectal carcinomas, and to explore its relationship with nuclear genetic alterations and its prognostic meaning. Eighty-three colorectal carcinomas with corresponding normal mucosa were analyzed for mtGI, nuclear microsatellite instability (nMSI), and loss of heterozygosity (LOH) of hMSH2, hMLH1, and p53 genes. Mitochondrial and nuclear alterations were examined for mutual correlation and for associations with clinicopathological features and clinical outcomes. Out of 83 cases, mtGI was identified in 23 carcinomas (27.7%), whereas nMSI was detected in 11 (13.3%). Of the 23 cases with mtGI, only two showed nMSI simultaneously. The frequencies of LOH of hMSH2, hMLH1, and p53 were 16.1%, 11.6%, and 65.3%, respectively. There was no significant association between mtGI and these allelic losses. Notably, Dukes’ C patients with mtGI had better disease-free and overall survival than those lacking this feature (p = 0.0516 and 0.0313, respectively). Mitochondrial genomic instability occurs with a high frequency in colorectal carcinomas but is independent of nMSI and allelic deletion of hMSH2, hMLH1, and p53 genes. The results suggest that, instead of nuclear MMR system, there might be different mechanisms involving mitochondrial genomic integrity, and mtGI confers a better prognosis in Dukes’ C colorectal cancer.

Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus, causing severe central nerve system diseases without specific treatments. The NS2B-NS3 protease of flaviviruses mediates several cleavages on the flavivirus polyprotein, being believed to be a target for antiviral therapy. NS2B is the cofactor of the viral serine protease, correlating with stabilization and substrate recognition of the NS3 protease. In this study, we investigate the functional determinants in the JEV NS2B for the activation of the NS3 protease. Cis- and trans-cleavage assays of the deletions at the N-terminal of NS2B demonstrated that the NS2B residues Ser(46) to Ile(60) were the essential region required for both cis and trans activity of the NS3 protease. In addition, alanine substitution at the residues Trp53, Glu55, and Arg56 in NS2B significantly reduced the cis- and trans-cleavage activities of the NS3 protease. Sequence alignment and modeled structures suggested that functional determinants at the JEV NS2B residues Ser46 to Ile60, particularly in Trp53, Glu55 and Arg56 could play an important configuration required for the activity of the flavivirus NS3 protease. Our results might be useful for development of inhibitors that block the interaction between NS2B and NS3.

Colorectal cancer (CRC) is one of the most common causes of cancer deaths in the world, and most of CRC arise sporadically by the emergence of multiple chromosomal aberrations. Allelic losses in the long arm of chromosome 4 are commonly encountered in many human malignancies, but few tumor suppressor genes (TSGs) are identified. By loss of heterozygosity study at chromosome 4 in 114 colorectal carcinomas, we identified N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4) as a novel TSG that had been published recently. We demonstrated for the first time that the genetic loss of NDST4 was significantly associated with advanced pathological stage and poorer overall survival of patients. Meanwhile, gene expression of NDST4 was obviously decreased in 30 (57.7%) of 52 CRC tumors when compared with their matched normal mucosae. In the present study, tumor suppressor activity of NDST4 was identified by in vitro cell models and mouse xenograft tumor models. Ectopic expression of NDST4 in the human CRC cells induced a significant suppression in cell proliferation, anchorage-independent growth and invasion in vitro. Subcutaneous injection of NDST4-inducible CRC cells in nude mice showed alleviated tumor growth in doxycycline treated mice when compared with doxycycline untreated mice. More importantly, via intrasplenic implantation, re-expression of NDST4 in silenced cells restrained liver metastasis, and also improved survival in nude mice. Taken together, these results indicate that NDST4 is a tumor suppressor gene associated with CRC, and its downregulation might promote tumor progression and distant metastasis. In addition, allelic loss of NDST4 gene could serve as a molecular predictor of metastasis and poor prognosis in patients with CRC. Citation Format: Tzu-Ming Jao, Ming-Hong Tsai, Sheng-Tai Tzeng, Ya-Chien Yang. N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4, a novel tumor suppressor, suppresses tumorigenesis and liver metastasis of colorectal cancer cells in mice. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2149. doi:10.1158/1538-7445.AM2015-2149

e14532 Background: Shisa3, a newly identified tumor suppressor, inhibits tumorigenesis and metastasis via counter-regulation of β-catenin in non-small cell lung cancer. However, its tumor suppresso...

This study proposes that culture moderates the relationship between individual factors and incivility. This occurs because some cultures allow for incivility while others constrain incivility more heavily. We tested our hypotheses with a sample of 268 MBAs and EMBAs from Taiwan and the United States. Our results showed that only among those who perceived low collectivism did individual achievement orientation relate positively to incivility. Similarly, the relationship of conflict self-efficacy (i.e., beliefs in ones skills in managing direct conflict) with incivility was lower for those higher in collectivism.

Obstructive colitis refers to ulceroinflammatory lesions that occur in the colon proximal to an obstructing lesion. As this condition is not widely appreciated by pathologists or clinicians, we describe herein a case of colonic polyposis and sigmoid colonic carcinoma with obstructive colitis. A 47-year-old Taiwanese woman presented to Cardinal Tien Hospital with a 3-day history of acute onset of abdominal pain, vomiting, and watery diarrhea. A lower gastrointestinal series using water-soluble contrast medium revealed annular narrowing of the sigmoid colon and showed polyposis at the rectosigmoid colon and regional colitis over the proximal descending colon. She was treated by total colectomy. Microscopic sections showed poorly differentiated adenocarcinoma, tubular adenomas, and a segment of obstructive colitis measuring 25 cm in length 5 cm proximal to the colon tumor. The tumor was also retrieved for simultaneous analyses of replication error and loss of heterozygosity. A total of three instances of loss of heterozygosity were demonstrated at the P53, MET, and D8S254 gene loci. No examples of replication error were detected. Obstructive colitis can cause diagnostic and therapeutic problems. Colitis areas may be a source for septicemia or may perforate and lead to peritonitis. The frequently normal appearance at surgery may lead to involved segments of colon being used for anastomoses with consequent complications. Awareness of the features and incidence of obstructive colitis should help physicians avoid these diagnostic and therapeutic problems.