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Enhancers are often mutated and dysregulated in various diseases such as cancer. By integrating the function annotation of the mammalian genome (FANTOM) enhancers expression profiles and RNA-seq data from The Cancer Genome Atlas (TCGA) of 13 cancers and their corresponding para-cancerous tissues, we systematically identified a total of 4702 significantly differentially expressed (DE) enhancers. Furthermore, a total of 1036 DE genes regulated by DE enhancers were identified. It was found that in these 13 cancers, most (61.13%) enhancers were ubiquitously expressed, whereas DE enhancers were more likely to be tissue-specific expressed, and the DE genes regulated by DE enhancers were significantly enriched in cancer-related pathways. Finally, it was manifested that 74 single nucleotide polymorphisms (SNPs) were located in 37 DE enhancers, and these SNPs affected the gain and loss of functional transcription factor binding sites of 758 transcription factors, which were shown to be highly correlated with tumorigenesis and development.

For the insufficient of students' innovative ability and employment ability caused by the imbalance between theory teaching and practice teaching in the process of applied undergraduate majors' personnel training, the author introduced a talent cultivation paths - the talent training mode based on the combination of professional programmatic accreditation and double certificates under the background of university-industry cooperation. The purpose is to promote balanced development of the theory teaching and practice teaching. Then, the operation mechanism of training mode was discussed from three aspects: the guarantee mechanism of alliance organization; the theory of professional competency standard system; and the cohesive mechanism of double certificate talent employment. Finally, the article introduced the successful practice of personnel training mode in engineering cost department, Tianjin University of Technology, and put forward some ideas about applied undergraduate majors' personnel training.