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The combination of aluminum and steel materials in hybrid structures shows promising applications in the automotive, marine, and aerospace sectors. The present study investigates how the tool–pin offset impacts the microstructural, mechanical, and electrochemical characteristics of friction stir-welded joints between dissimilar AA2024-T3 and SS304 materials. The optimum conditions were achieved at a tool rotation of 560 r/min, a traverse speed of 25 mm/min, and a tool–pin offset of 1 mm towards the Al side. Upon increasing the tool–pin offset to 1.5 mm, insufficient heat input resulted in inadequate plastic deformation and material flow, leading to the creation of flaws like voids, tunnels, and interfacial gaps. However, under optimal conditions, the joint exhibited a well-defined and serrated interface, with fine steel fragments securely embedded in the Al matrix. Additionally, thin intermetallic compounds were observed around the steel fragments within the Al matrix. This favorable combination resulted in enhanced tensile strength and increased ductility in the joint. The Vickers hardness test revealed that the stir zone exhibited the highest hardness values, primarily attributed to the ultra-refinement of grains. Furthermore, the potentiodynamic test revealed that the welded samples show improved corrosion resistance against the base material AA2024-T3, although BM-SS304 exhibited the highest corrosion resistance among all the samples, likely due to its higher chromium content. [ABSTRACT FROM AUTHOR]

With the elimination of solidification defects, friction stir welding becomes the most effective process for joining flat and curved surfaces. Tool geometry (tool pin profile, shoulder diameter) and tool rotation speed mainly contribute to the overall weld quality. The study evaluates the simultaneous effect of tool geometry, rotational speed, base offset and double pass Friction Stir Welding (DP-FSW) on the defect formation and the mechanical properties. Micro-structure and macro-structure have been analysed for weld strength and hardness characterisation, and found, at high tool rotation speed maximum hardness is achieved in the Stir Zone (SZ). A small tunnel present near the middle proved to be more detrimental than the large one at the bottom. Grain refinement in the SZ was 87.2% while augmentation in hardness was 21.3 HV. Maximum tensile strength of approximately 103% with decent bead profile is obtained, using taper cylindrical tool at 710 rpm with 14 mm shoulder diameter. Fractography of the fractured surface revealed a pure ductile failure. It has been discovered that with base offset the strength of the weld is increased. However, reduction of 4.8% was obtained with DP-FSW. Better weld profile gives better strength. With DP-FSW, a significant improvement in bead surface is obtained. [ABSTRACT FROM AUTHOR]

Introduction Tuberculosis causes significant human mortality and morbidity worldwide, with the lung granuloma representing the typical site of disease manifestation (1, 2). Granulomas are stratified structures, containing concentric layers of [...], The hallmark of human Mycobacterium tuberculosis infection is the presence of lung granulomas. Lung granulomas can have different phenotypes, with caseous necrosis and hypoxia present within these structures during active tuberculosis. Production of NO by the inducible host enzyme NOS2 is a key antimycobacterial defense mechanism that requires oxygen as a substrate; it is therefore likely to perform inefficiently in hypoxic regions of granulomas in which M. tuberculosis persists. Here we have used [Nos2.sup.-/-] mice to investigate host-protective mechanisms within hypoxic granulomas and identified a role for host serine proteases in hypoxic granulomas in determining outcome of disease. [Nos2.sup.-/-] mice reproduced human-like granulomas in the lung when infected with M. tuberculosis in the ear dermis. The granulomas were hypoxic and contained large amounts of the serine protease cathepsin G and clade B serine protease inhibitors (serpins). Extrinsic inhibition of serine protease activity in vivo resulted in distorted granuloma structure, extensive hypoxia, and increased bacterial growth in this model. These data suggest that serine protease activity acts as a protective mechanism within hypoxic regions of lung granulomas and present a potential new strategy for the treatment of tuberculosis.

An important role of transforming growth factor-β (TGF-β) in the development of regulatory T cells is well established. Although integrin-mediated activation of latent TGF-β1 is considered essential for the induction of regulatory T (Treg) cells by antigen-presenting cells (APCs), such an activation mechanism is not applicable to the TGF-β2 isoform, which lacks an integrin-binding RGD sequence in its latency-associated peptide. Mucosal and ocular tissues harbour TGF-β2-expressing APCs involved in Treg induction. The mechanisms that regulate TGF-β activation in such APCs remain unclear. In this study, we demonstrate that murine APCs exposed to TGF-β2 in the environment predominantly increase expression of TGF-β2. Such predominantly TGF-β2-expressing APCs use thrombospondin- 1 (TSP-1) as an integrin-independent mechanism to activate their newly synthesized latent TGF-β2 to induce Foxp3+ Treg cells both in vitro and in vivo. Expression of Treg induction by TGF-β2-expressing APCs is supported by a TSP-1 receptor, CD36, which facilitates activation of latent TGF-β during antigen presentation. Our results suggest that APC-derived TSP-1 is essential for the development of an adaptive regulatory immune response induced by TGF-β2-expressing APCs similar to those located at mucosal and ocular sites. These findings introduce the integrin-independent mechanism of TGF-β activation as an integral part of peripheral immune tolerance associated with TGF-β2-expressing tissues. [ABSTRACT FROM AUTHOR]