1. Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation
- Author
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Nozawa, R. S., Nagao, K., Masuda, H. T., Iwasaki, O., Hirota, T., Nozaki, N., Kimura, Hiroshi, and Obuse, C.
- Subjects
Chromosomal Proteins, Non-Histone ,Immunoprecipitation ,Blotting, Western ,Aurora B kinase ,Mitosis ,Transposases ,Plasma protein binding ,Chromatids ,Protein Serine-Threonine Kinases ,Biology ,Mass Spectrometry ,Cell Line ,Chromatids/genetics/metabolism ,Chromatin/metabolism ,Aurora Kinases ,Two-Hybrid System Techniques ,Aurora Kinase B ,Humans ,Kinetochores ,Zinc finger ,Mitosis/genetics/*physiology ,Fluorescence recovery after photobleaching ,Cell Biology ,Molecular biology ,Chromatin ,Cell biology ,Kinetochores/metabolism ,Microscopy, Fluorescence ,Chromobox Protein Homolog 5 ,Chromosomal Proteins, Non-Histone/genetics/*metabolism ,Protein-Serine-Threonine Kinases/*metabolism ,Heterochromatin protein 1 ,RNA Interference ,Transposases/genetics/*metabolism ,Fluorescence Recovery After Photobleaching ,HeLa Cells ,Protein Binding - Abstract
Heterochromatin protein 1 (HP1) has an essential role in heterochromatin formation and mitotic progression through its interaction with various proteins. We have identified a unique HP1alpha-binding protein, POGZ (pogo transposable element-derived protein with zinc finger domain), using an advanced proteomics approach. Proteins generally interact with HP1 through a PxVxL (where x is any amino-acid residue) motif; however, POGZ was found to bind to HP1alpha through a zinc-finger-like motif. Binding by POGZ, mediated through its zinc-finger-like motif, competed with PxVxL proteins and destabilized the HP1alpha-chromatin interaction. Depletion experiments confirmed that the POGZ HP1-binding domain is essential for normal mitotic progression and dissociation of HP1alpha from mitotic chromosome arms. Furthermore, POGZ is required for the correct activation and dissociation of Aurora B kinase from chromosome arms during M phase. These results reveal POGZ as an essential protein that links HP1alpha dissociation with Aurora B kinase activation during mitosis.
- Published
- 2010