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The Total Quality Management (TQM) paradigm encompasses adapted and developed management techniques, emphasizing process management, leadership, strategic planning, customer focus, supply management, human resources management, and quality indicators. This research focuses on applying a TQM-based model to analyze relationships among TQM criteria in university student associations. Specifically, it explores students' perceptions of process approach-based thinking as a catalyst for organizational and quality indicators in these associations. The study, involving 700 respondents, utilized Structural Equation Modeling (SEM), incorporating confirmatory factor analysis and path analysis. Key findings reveal that process management positively influences all TQM elements, while only leadership and human resources management directly impact quality indicators. The study's outcomes have the potential to enhance decision-making processes in universities and student associations, thereby improving academic and future business life quality.

BACKGROUND: Postoperative complications of colorectal cancer surgery contribute to increased morbidity and mortality in patients. OBJECTIVES: Investigate the role of biochemical markers in serum and peritoneal fluid in the development of postoperative complications in patients with enteral anastomosis. DESIGN: Prospective. SETTING: University hospitals. PATIENTS AND METHODS: The studied population consisted of patients who underwent surgical treatment with created anastomosis or Hartmann's resection from April 2022 to April 2024, conducted at the Clinical-Hospital Center Kosovska Mitrovica and the University Clinical Center Kragujevac. Spearman's correlation coefficient (rs) was used to test associations between categorical variables. MAIN OUTCOME MEASURES: Lactate, albumin, lactate dehydrogenase, and IgA antibodies were monitored as predictors of anastomotic dehiscence and general postoperative complications. SAMPLE SIZE: 52 RESULTS: The concentration of lactate in the drain fluid on the third postoperative day was statistically significantly higher in patients who did not develop anastomotic dehiscence (P=.006). The concentration of IgA antibodies in the drain fluid on the third and fifth days post-surgery showed a moderate negative correlation with lactate concentration (rs=-.670, P=.012; rs=-.577, P=.039), repectively. There was a significantly higher concentration of albumin in the drain fluid on the third day post-surgery in patients who developed dehiscence (P=.040), and on the seventh day post-surgery in those who did not develop dehiscence (P=.001). The concentration of LDH on the third day in the drain fluid after surgery was statistically significantly higher in patients who did not develop dehiscence (P=.020). There was a statistically significant difference in lactate concentration in the drain fluid on the third (P

This case is about a 20-year-old woman with tuberous sclerosis complex and psychosis admitted to the psychiatry clinic of UMHAT Aleksandrovska and treated with zuclopenthixol. She experienced paranoid delusions and verbal and physical aggression towards her mother. Because of side effects, the treatment with risperidone was changed to zuclopenthixol. We evaluated her condition with BPRS24. The score of BPRS24 in admission to the hospital was 97 points. At discharge, the score of BPRS24 was 54 points. After a month, the BPRS24 score was reduced to 39 points; two months later, it was 37.

Abstract Zeaxanthin, a vital dietary carotenoid, is naturally synthesized by plants, microalgae, and certain microorganisms. Large-scale zeaxanthin production can be achieved through plant extraction, chemical synthesis, or microbial fermentation. The environmental and health implications of the first two methods have made microbial fermentation an appealing alternative for natural zeaxanthin production despite the challenges in scaling up the bioprocess. An intermediate between β-carotene and zeaxanthin, β-cryptoxanthin, is found only in specific fruits and vegetables and has several important functions for human health. The low concentration of β-cryptoxanthin in these sources results in low extraction yields, making biotechnological production a promising alternative for achieving higher yields. Currently, there is no industrially relevant microbial fermentation process for β-cryptoxanthin production, primarily due to the lack of identified enzymes that specifically convert β-carotene to β-cryptoxanthin without further conversion to zeaxanthin. In this study, we used genetic engineering to leverage the oleaginous yeast Yarrowia lipolytica as a bio-factory for zeaxanthin and β-cryptoxanthin production. We screened 22 β-carotene hydroxylases and identified eight novel enzymes with β-carotene hydroxylating activity: six producing zeaxanthin and two producing only β-cryptoxanthin. By introducing the β-carotene hydroxylase from the bacterium Chondromyces crocatus (CcBCH), a β-cryptoxanthin titer of 24 ± 6 mg/L was achieved, representing the highest reported titer of sole β-cryptoxanthin in Y. lipolytica to date. By targeting zeaxanthin-producing β-carotene hydroxylase to the endoplasmic reticulum and peroxisomes, we increased the production of zeaxanthin by 54% and 66%, respectively, compared to untargeted enzyme. The highest zeaxanthin titer of 412 ± 34 mg/L was achieved by targeting β-carotene hydroxylases to peroxisomes. In addition, by constructing multienzyme scaffold-free complexes with short peptide tags RIDD and RIAD, we observed a 39% increase in the zeaxanthin titer and a 28% increase in the conversion rate compared to the strain expressing unmodified enzyme. The zeaxanthin titers obtained in this study are not the highest reported; however, our goal was to demonstrate that specific approaches can enhance both titer and conversion rate, rather than to achieve the maximum titer. These findings underscore the potential of Y. lipolytica as a promising platform for carotenoid production and provide a foundation for future research, where further optimization is required to maximize production.

Introduction/purpose: Intralogistics involves the organization, control, realization, and optimization of material and information flows within a technologically integrated system. The selection and application of appropriate material handling equipment (MHE) play a crucial role in shaping intralogistics systems. This paper aims to define guidelines for selecting MHE based on scientific research. The methodology implemented to achieve this goal comprises several fundamental steps. Methods: The initial step involves analyzing three typical groups of tasks in (intra)logistics systems: transshipment, internal transport, and production activities. The analysis focuses on identifying and describing key task parameters, such as the type of unit load, quantity, flow intensity, and the location and timing of task initiation and completion. Next, there is a step devoted to the analysis of relevant scientific research. The literature is searched according to specific criteria, such as publication year, keywords, and citation count, to form a database of relevant papers. The analysis focuses on identifying and linking the parameters of practical tasks with those described in the literature and on the characteristics of the MHE used to facilitate the selection of appropriate MHE for these tasks. Results: Following this, in the third step, the observed task is classified according to standard task types found in the literature. Conclusion: Finally, in the last/fourth step, regression analysis is applied to define the guidelines for MHE selection based on the frequency of use of specific MHE groups for certain task groups as described in the literature.

Aim: This study aimed at identifying factors that worsen the prognosis of diabetic foot, one of the most common complications seen in patients with diabetes. Materials and patients: The sample included 230 patients treated in the Department of Surgery at Kaspela University Hospital in Plovdiv, Bulgaria, between January 2016 and May 2022. Of them, 55.65% were men, and 44.35% were women. The age of the patients ranged from 18 to 77 years, with a mean age of 44.4±14.7 years. Results: Diabetic foot patients of grade ≥4 were 1.8 times more likely to be amputated than the rest of the patients (AOR=1.6; 95% CI: 1.504, 4.683). The utilization of inappropriate antibiotics was associated with a 9.47-fold increased risk of amputation (p

An advanced flexible programming methodology for CNC woodworking machines was developed. As the research starting base, a three-axis CNC woodworking machine was used. The developed methodology is proposed for programming, simulation, postprocessing, and machining by woodworking machine. This flexible programming method integrates the standard programming based on CAD, CAD/CAM systems, and STEP-NC protocol through different output files, enabling data interoperability during the realization of the machining tasks. The control system for the machine is configured based on the open-architecture software LinuxCNC to verify the flexible programming method and the results obtained. Programming verification was realized by simulation on a configured virtual machine in different programming environments and finally on a virtual machine integrated with the control system. The results obtained from the study were evaluated comparatively.

In this paper, the effect of radiation hazards on the environment while using the non-destructive radiography method was investigated. The research was carried out by measuring the radiation amounts at 0.5 and 2 m distance from the exposure container with radioactive iridium-192 isotope used for tests with the radiography method. The tests were carried out in different positions in relation to the protective bunkers used in the company STSI Ltd. (Zagreb, Croatia) to reduce the radiation emission of the used radioactive isotope. The effectiveness of the protective bunkers in terms of reducing radiation in the environment is determined. In addition to examining the effects and hazards of radiation, the procedure for proper disposal of hazardous and non-hazardous waste generated by the non-destructive radiography method is described. Research has shown that the storage of exposure containers with radioactive iridium-192 isotope in the large protective bunker and the mini-bunker contributes to the improvement of environmental and personnel protection. The disposal of hazardous and non-hazardous waste after testing with the non-destructive radiography method has also proven to be of enormous importance for environmental protection.

Abstract We analyzed data from positively tested COVID-19 outpatients to describe self-medication with OTC drugs and use of other remedies against symptoms of SARS-CoV-2 infection. We specifically considered their type and frequency, as well as associations with patient characteristics, and reasons for use. Data were collected between May 1, 2020 and February 22, 2021 with two questionnaires in an observational cohort study with PCR-confirmed SARS-CoV-2-positive adult outpatients in the district of Western Pomerania in Germany. 523 out of 710 outpatients (74%; 340 women and 183 men) reported using drugs and other remedies to relieve COVID-19-symptoms. Overall, participants reported utilization of 1282 finished dosage products or remedies, including 213 different ingredients. In the population of 710 outpatients, utilization of ibuprofen (26%), acetaminophen (21%), metamizole (14%), and acetylsalicylic acid (10%) was most commonly reported. Phytopharmaceuticals, herbal and animal products as well as vitamins and minerals were also frequently reported. Among the 523 participants who used drugs and other remedies, most commonly mentioned reasons for use were headache (40%), other kinds of pain (e.g. myalgia; 37%), fever (24%) and cough (16%). Our analysis showed that a majority of the participants tried to alleviate COVID-19-symptoms using drugs and other remedies. Especially analgesic and antipyretic agents, followed by herbal medicines, were used very frequently. Trial registration: German Register for Clinical Studies DRKS00021672, first registration on December 1st, 2020.

Introduction: Osteoarthritis is a prevalent degenerative joint disorder associated with pain and functional impairment. Curcumin, a natural anti-inflammatory compound, has garnered attention for its potential therapeutic benefits in osteoarthritis management. Aim: This study aimed to investigate the correlations between curcumin use, patient characteristics, medication use, and changes in health outcomes among osteoarthritis patients. The primary hypothesis was that curcumin use would correlate with positive changes in health metrics. Materials and methods: A cohort of 60 osteoarthritis patients was enrolled in this observational study and divided into 4 groups according to the type of osteoarthritis. Patient selection criteria included a diagnosis of osteoarthritis and consent for curcumin supplementation. The primary outcome variable was changes in health metrics measured across three visits. Body mass index, nonsteroidal anti-inflammatory drugs (NSAIDs) and paracetamol use were also recorded. Correlation analysis and paired t-tests were employed to examine the relationships between variables and changes in health metrics. Results: Correlation analyses revealed a weak negative correlation between BMI and changes between ‘Visit 1’ and ‘Visit 2’ (r=−0.09). NSAIDs use exhibited a weak positive correlation with changes during this period (r=0.17), while paracetamol use displayed weak correlations (r=−0.05) with changes. Statistically significant changes were observed between ‘Visit 1’ and ‘Visit 2’ (mean change =−5.93, p

European grayling, Thymallus thymallus (Linnaeus, 1758) (Actinopterygii: Salmoniformes: Salmonidae), represents one of the highly attractive riverine fishing species in Europe. Its populations have declined in the Czech Republic due to various adverse factors. Current approaches for strengthening these populations based on restocking with artificially reared 1+ or 2+ old individuals have proven to be ineffective. This study focuses on the possibilities of supporting or restoring these populations by reintroducing two-month-old, fast-growing fry reared in ponds. In June 2021, 5400 advanced fry marked with Alizarin Red (ARS) were introduced into three free-flowing South Bohemian streams. The recapture rates and biometric data (length and weight) of stocked grayling were assessed at the release sites as well as further downstream. The first electrofishing monitoring was conducted at the end of the growing season (October) and after their first winter (March). Water temperature and flow rate at the stocking sites were monitored throughout the growing season. The substantial number of recaptured individuals across all monitored sites suggests that the fry successfully adapted to their new habitat and significantly contributed to the composition of local fish communities. Downstream movement of stocked fry correlated with lower water temperatures and higher flows. Our findings demonstrate that some introduced individuals successfully overwintered at all three reintroduction sites. Marking with ARS has proven to be a very effective non-invasive method of group marking juvenile fish and is suitable for monitoring stocking programs. The production and stocking of fast-growing advanced fry of European grayling is thus a promising strategy for revitalizing and strengthening the populations of this threatened fish in running waters.

AIM: To invastigate intra- and interobserver reliability of interferometry, tear meniscus height (TMH) measurement and meibography (MBG) of an ocular surface analyzer, LacryDiag (Quantel Medical, France). METHODS: Five consecutive measurements and subsequent analysis of interferometry, TMH, and MBG were recorded by two examiners using the LacryDiag. To assess intra- and interobserver reliability, we used Cohen's kappa for categorical variables (interferometry), or intraclass correlation coefficient for continuous variables (TMH, MBG). RESULTS: Thirty eyes of 30 examinees were included. For both observers, there was excellent intraobserver reliability for MBG (0.955 and 0.970 for observer 1 and 2, respectively). Intraobserver reliability for observer 1 was substantial for interferometry (0.799), and excellent for TMH (0.863). Reliability for observer 2 was moderate for interferometry (0.535) and fair to good for TMH (0.431). Interobserver reliability was poor for interferometry (0.074) and fair to good for TMH (0.680) and MBG (0.414). CONCLUSION: LacryDiag ocular surface analyzer in our study proves to be a reliable noninvasive tool for the evaluation of TMH and MBG. As for interferometry, poor interobserver reliability, fair to good intraobserver reliability for observer 1, and moderate for observer 2, leave room for improvement.

The purpose of the present study was to compare game-related statistics between men's and women's under-17 (U-17) basketball teams and to identify game-related statistics that discriminate between winning and losing teams for both sexes. Games with a final score difference greater than 30 points were excluded from the analysis, so the final sample consisted of 196 games (109 men's and 87 women's games) from the 2022/2023 season of the Triglav Cadet League of Serbia. The following game-related statistics were gathered from the official box scores of the Basketball Federation of Serbia: 2- and 3-point field-goals (successful and unsuccessful), free throws (successful and unsuccessful), defensive and offensive rebounds, assists, steals, turnovers, blocks (committed and received), and fouls (committed and received). Men's teams had significantly better values than women's teams in successful and unsuccessful 2-point field-goals, successful 3-point field-goals, successful and unsuccessful free throws, assists, and turnovers. Women's teams had statistically better values than men's teams in unsuccessful 3-point field-goals, offensive rebounds, steals, and committed fouls. The discriminant factors between winning and losing teams in close games, were successful 2-point field-goals, defensive rebounds and assists for both sexes, and offensive rebounds for men's teams. In balanced games, discriminant factors were assists for both sexes and successful 2-point field-goals for women's teams. In unbalanced games, discriminant factors were assists for both sexes, defensive rebounds for men's teams, and successful 2-point field-goals for women's teams. These findings suggest that there are notable differences in game-related statistics for U-17 men's and women's basketball teams.

Abstract In this article, densities (and their derivatives) of subordinators and inverse subordinators are considered. Under minor restrictions, generally milder than the existing in the literature, employing a useful modification of the saddle point method, we obtain the large asymptotic behaviour of these densities (and their derivatives) for a specific region of space and time and quantify how the ratio between time and space affects the explicit speed of convergence. The asymptotics is governed by an exponential term depending on the Laplace exponent of the subordinator and the region represents the behaviour of the subordinator when it is atypically small (the inverse one is larger than usual). As a result, a route to the derivation of novel general or particular fine estimates for densities with explicit constants in the speed of convergence in the region of the lower envelope/the law of iterated logarithm is available. Furthermore, under mild conditions, we provide a power series representation for densities (and their derivatives) of subordinators and inverse subordinators. This representation is explicit and based on the derivatives of the convolution of the tails of the corresponding Lévy measure, whose smoothness is also investigated. In this context, the methods adopted are based on Laplace inversion and strongly rely on the theory of Bernstein functions extended to the cut complex plane. As a result, smoothness properties of densities (and their derivatives) and their behaviour near zero immediately follow.

Summary: Background: Post-traumatic stress disorder (PTSD) and depression are common after mild traumatic brain injury (mTBI), but their biological drivers are uncertain. We therefore explored whether polygenic risk scores (PRS) derived for PTSD and major depressive disorder (MDD) are associated with the development of cognate TBI-related phenotypes. Methods: Meta-analyses were conducted using data from two multicenter, prospective observational cohort studies of patients with mTBI: the CENTER-TBI study (ClinicalTrials.gov ID NCT02210221) in Europe (December 2014–December 2017) and the TRACK-TBI study in the US (March 2014–July 2018). In both cohorts, the most common causes of injury were road traffic accidents and falls. Primary outcomes, specifically probable PTSD and depression, were defined at 6 months post-injury using scores ≥33 on the PTSD Checklist-5 and ≥15 on the Patient Health Questionnaire-9, respectively. We calculated PTSD-PRS and MDD-PRS for patients aged ≥17 years who had a Glasgow Coma Scale score of 13–15 upon hospital arrival and assessed their association with PTSD and depression following TBI. We also evaluated the transferability of the findings in a cohort of African Americans. Findings: Overall, 11.8% (219/1869) and 6.7% (124/1869) patients were classified as having probable PTSD and depression, respectively. The PTSD-PRS was significantly associated with higher adjusted odds of PTSD in both cohorts, with a pooled odds ratio (OR) of 1.55 [95% confidence interval (CI) 1.30–1.84, p

This anthropological analysis focuses on the Croatian feature film Night Boats (2012), directed by Igor Mirković. The subject of the movie is a partnership that develops between two residents of a nursing home in Zagreb, highlighting, among other things, numerous problems and difficulties that the elderly face while trying to pursue a new romantic relationship at the very end of their lives, in specific nursing home conditions. The couple’s escape from the nursing home serves as an act of resistance, leaving room for the analysis of visual depictions of the various deprivations encountered by the elderly. Depictions of physical, emotional and economic dependency which result in an inability to make independent decisions, are in accordance with the results attained during several years of field research conducted in nursing homes in the Republic of Serbia. This research, which explores new partnerships as strategies of action among nursing home residents, will be compared with the film’s visual interpretations. Interpreting the visual depiction of deprivation and infirmity, which society associates the elderly with, will also include a semiotic interpretation of the symbolic expression that is used in the movie.

Viral infections can lead to platelet activation and hemostatic complications. However, the extent to which platelet reactivity remains altered after convalescence, contributing to long-term health impairments as observed after COVID-19 is not yet fully understood. Therefore, we conducted a cohort study (DRKS00025217) to determine platelet function in individuals convalesced from mild COVID-19. Assays were performed ex vivo with blood from convalescents at 2–15 weeks and 6–10 months after convalescence, focusing on platelet aggregation, activation markers, and thrombin formation. In addition, two other potentially relevant factors for platelet function were examined: the immunomodulatory mediator sphingosine-1-phosphate (S1P) and the platelet expression of the transporter MRP4 (ABCC4). Our findings indicate that robust platelet functions, including platelet aggregation determined by light transmission aggregometry, and thrombin formation, were not altered in convalescents compared to matched control individuals. However, an elevation in subtle platelet activation markers, such as P-selectin surface expression and activation of glycoprotein IIb/IIIa, was observed 2–15 weeks after convalescence. This was accompanied by an increased expression of MRP4 in platelets and significantly elevated levels of S1P in platelet-poor plasma. Our findings suggest increased platelet sensitization and a pro-inflammatory state even after convalescence from mild COVID-19, pointing toward MRP4 and S1P as associated factors.

Community engagement is essential for creating social value and fostering high-quality development in megaprojects. However, organizing such engagement within public-private partnerships is particularly complex in weak institutional contexts, such as those found in sub-Saharan Africa. Existing research often depoliticizes community engagement, overlooking the critical role of government. Additionally, empirical studies on this phenomenon in sub-Saharan Africa remain scarce. In this study, we adopt a governmentality lens and a design science research method to identify meta-organizing and responsibilization as governmental strategies for organizing community engagement in weak institutional contexts, such as those in sub-Saharan Africa. Through an embedded case study of a megaproject in Nigeria, we develop two sets of design principles that form a transformational community engagement framework. Our findings have implications for community engagement practices in megaprojects within weak institutional contexts and contribute to mainstream external stakeholder engagement research.

This paper approaches the connection between musical constructs and visuo-haptic experience through the lens of the cognitive-linguistic notion of the “image schema.” The proposal is that the subconscious inference of spatial and haptic schematic constructs in music, such as vertical movement, will motivate their equally common occurrence in the language about that music, irrespective of the fact that this language never describes the musical structure in a one-to-one fashion. We have looked for five schemas in the scores for the first ten piano sonatas by Ludwig van Beethoven and three famous analytical and pedagogical texts about them: force, indicating changes in musical dynamics and referential invocation of power-related terms in the books; path, identifying vertical movement in the music and suggestions of upward- or downward motion in the texts; link, suggesting the presence or absence of musical slurs and references to attachment or detachment in the language; balance, indicating the loss and regain of consonance in the harmony and invocation of lost and recovered stability in the verbal semantics; and containment, allocating the nonharmonic tones that “belong” to their resolving notes in the scores and referring to physical or metaphorical enclosed areas in the texts. Results of the corpus analysis suggest the following conclusions: musical schemas outnumber linguistic ones sevenfold; moderate schema strengths are typical of both language and music; predominant valences are shared by language and music in three schemas out of five; hierarchies of five schemas by strength differ, though the strongest schemas are mostly shared. Yet the central finding is that the correlations between each schema pair for music and language, by scalarity and valence, are total. This implies that (1) schemas operate as semantic building blocks irrespective of the external “symbolical form” in which they are realized and (2) scalarized image schema complexes perceived in one cognitive mode may motivate the emergence of a corresponding number of the same complexes in another.

Obravnavamo slovenske količinske zgradbe z ločilnim števnikom dvoj(e) in samostalnikom. Predstavljene in pokomentirane so dosedanje obravnave tega števnika v še aktualnih slovenskih slovnicah, v slovarju slovenskega knjižnega jezika in slovenskem pravopisu. Analizirana je raba zgradb z dvoj(e) s samostalnikom v besedilnem korpusu Gigafida 2.0, predstavljena in pokomentirana je družljivost števnika dvoje z različnimi tipi samostalnikov.

BackgroundHigh-intensity interval training (HIIT) in school settings has been much less studied in adolescents. The aim of this study was to examine the impact of HIIT on health-related fitness in adolescents.MethodsThe total sample consisted of 60 adolescents (age 16.33 ± 0.62 years) from secondary Grammar school, randomly divided into two groups: the experimental (EG) (30) and the control group (CG) (30). The experimental program (12-weeks; 2 times per week) involved two Tabata sessions during one physical education class lasting 4 min each. Participants were tested for health-related fitness components-cardiorespiratory fitness (The Shuttle Run Test (SRT) and strength, hand grip test, standing long jump (SLJ) and counter movement jump (CMJ).ResultsBoth the EG and the CG experienced significant positive changes in SRT (meters) and VO2max values compared to baseline value (p < 0.05), however, the increase in the EG was significantly higher than that in the CG (SRT - ηp2 = 0.111; VO2max - ηp2 = 0.111, p < 0.01). The EG showed significant improvement in SRT (meters) and VO2max values compared to the CG (p < 0.01). Regarding the hand grip test results, a significant time × group interaction was found only for right hand (p < 0.01). Moreover, the improvements in SLJ and CMJ values was greater in EG than that in the CG group (SLJ- ηp2 = 0.182; CMJ- ηp2 = 0.112, p < 0.01).ConclusionFindings indicate that HIIT implemented into physical education classes can result in significant improvements in selected health related fitness components in adolescents.

Abstract A theoretical perspective on grandiose narcissism suggests four forms of it (sanctity, admiration, heroism, rivalry) and states that these forms conduce to different ways of thinking and acting. Guided by this perspective, we examined in a multinational and multicultural study (61 countries; N = 15,039) how narcissism forms are linked to cognitions and behaviors prompted by the COVID-19 pandemic. As expected, differences in cognitions and behaviors across narcissism forms emerged. For example, higher narcissistic rivalry predicted lower likelihood of enactment of COVID-19 prevention behaviors, but higher narcissistic sanctity predicted higher likelihood of enactment of COVID-19 prevention behaviors. Further, whereas the heroism, admiration, and rivalry narcissism forms acted in a typically antisocial manner, with high narcissism predicting greater endorsement of unfounded health beliefs, the sanctity form acted in a prosocial manner, with higher narcissism being linked to lower endorsement of unfounded COVID-19 health beliefs. Thus, the findings (a) support the idea of four narcissism forms acting differently, and (b) show that these differences reflect a double-edged sword, sometimes linking to an anti-social orientation, and sometimes linking to a pro-social orientation.

Abstract Background Limonene has a variety of applications in the foods, cosmetics, pharmaceuticals, biomaterials, and biofuels industries. In order to meet the growing demand for sustainable production of limonene at industry scale, it is essential to find an alternative production system to traditional plant extraction. A promising and eco-friendly alternative is the use of microbes as cell factories for the synthesis of limonene. Results In this study, the oleaginous yeast Yarrowia lipolytica has been engineered to produce d- and l-limonene. Four target genes, l- or d-LS (limonene synthase), HMG (HMG-CoA reductase), ERG20 (geranyl diphosphate synthase), and NDPS1 (neryl diphosphate) were expressed individually or fused together to find the optimal combination for higher limonene production. The strain expressing HMGR and the fusion protein ERG20-LS was the best limonene producer and, therefore, selected for further improvement. By increasing the expression of target genes and optimizing initial OD, 29.4 mg/L of l-limonene and 24.8 mg/L of d-limonene were obtained. We also studied whether peroxisomal compartmentalization of the synthesis pathway was beneficial for limonene production. The introduction of d-LS and ERG20 within the peroxisome improved limonene titers over cytosolic expression. Then, the entire MVA pathway was targeted to the peroxisome to improve precursor supply, which increased d-limonene production to 47.8 mg/L. Finally, through the optimization of fermentation conditions, d-limonene production titer reached 69.3 mg/L. Conclusions In this work, Y. lipolytica was successfully engineered to produce limonene. Our results showed that higher production of limonene was achieved when the synthesis pathway was targeted to the peroxisome, which indicates that this organelle can favor the bioproduction of terpenes in yeasts. This study opens new avenues for the efficient synthesis of valuable monoterpenes in Y. lipolytica.

The ongoing growth of artificial intelligence (AI) involves virtually every aspect of oncologic care in medicine. Although AI is in its infancy, it has shown great promise in the diagnosis of oncologic urological conditions. This paper aims to explore the expanding role of artificial intelligence in the histopathological diagnosis in urological oncology. We conducted a focused review of the literature on AI in urological oncology, searching PubMed and Google Scholar for recent advancements in histopathological diagnosis using AI. Various keyword combinations were used to find relevant sources published before April 2nd, 2024. We approached this article by focusing on the impact of AI on common urological malignancies by incorporating the use of different AI algorithms. We targeted the capabilities of AI’s potential in aiding urologists and pathologists in histological cancer diagnosis. Promising results suggest AI can enhance diagnosis and personalized patient care, yet further refinements are needed before widespread hospital adoption. AI is transforming urological oncology by improving histopathological diagnosis and patient care. This review highlights AI’s advancements in diagnosing prostate, renal cell, and bladder cancer. It is anticipated that as AI becomes more integrated into clinical practice, it will have a greater influence on diagnosis and improve patient outcomes.

Bouveret’s syndrome (BS) represents an exceedingly rare clinical entity characterized by gastric outlet obstruction induced by a gallstone passing through a cholecystoduodenal, cholecystogastric or choledochoduodenal fistula and impacting in the duodenum or pylorus. Endoscopy is the preferred first-line therapy. It has a favorable safety profile, but requires high level of expertise to achieve stone clearance. We report here the case of a 75-year-old Caucasian male who presented with vomiting and upper gastrointestinal bleeding and was diagnosed with BS by upper endoscopy. A single session stone extraction utilizing mechanical lithotripsy and laser lithotripsy was executed. Complete clearance of the duodenum and gallbladder was achieved, extracting successfully all fragments larger than 5 mm. The rarity of BS and its non-specific presentation makes it largely overlooked leading commonly to delayed diagnosis, which results in a high mortality rate of 12-30%. Computer tomography is the diagnostic method of choice. Upper endoscopy can establish the presence of obstruction, but fails to visualize a stone in up to 1/3 of the cases. Management is either endoscopic or surgical. Endoscopic treatment has a good safety profile (mortality 1.6% vs. 17.5-25% for surgery) but poor success rate – 43% vs. 94.1%. To achieve higher success rate, more advanced techniques are required such as mechanical or laser lithotripsy. Laser lithotripsy is a feasible option for endoscopic treatment of BS, but the duration of the procedure might be an issue in frail patients.

Background. It is of great importance that the training process of youth water polo players be tailored to didactic principles and developmental characteristics. However, despite this importance, there is a dearth of pertinent studies. Objectives. The study aimed to determine and explain the appropriate age for beginning of learning tactical elements in water polo, as stated by experts’ coaches’ opinion in this sport. Materials and methods. Accordingly, 27 expert water polo coaches completed a novel questionnaire, specifically designed for this study. Results. Test-retest reliability indicated satisfactory scores (r ranged from 0.85 to 1.00 with p

The study aims to monitor whether the implementation of investment projects in the 2014-2020 programming period is in line with the approved national strategic framework for economic integration of the regions in Bulgaria. The focus of the report is on financial instruments as an alternative form of financing. A method of deduction was used to describe the financial instruments. In order to investigate the effectiveness of the implementation of investments through them, an analysis of 10 selected municipal projects was made by tracking the progress of the financial resource absorption on a quarterly basis. From the official website of the Institute for Market Economics, data on the level of asymmetry of the different regions in terms of investments made and infrastructure are obtained. As a result of the study, it was found that despite the successful implementation of the Operational Programme "Regions in Growth" 2014-2020, the imbalance in the development of the different regions in Bulgaria remains significant. The lack of sufficient administrative capacity of municipalities and various procedural delays make municipal projects financed by the financial instrument unfeasible within the deadlines set for them. The findings of the study could be useful while new financial instruments in Bulgaria are implementing. The increased risk of non-compliance with municipal projects has be taken into consideration along with targeted action for the economic and social integration of Bulgaria's lagging regions

Abstract Background Mosquitoes pose a risk to human health worldwide, and correct species identification and detection of cryptic species are the most important keys for surveillance and control of mosquito vectors. In addition to traditional identification based on morphology, DNA barcoding has recently been widely used as a complementary tool for reliable identification of mosquito species. The main objective of this study was to create a reference DNA barcode library for the Croatian mosquito fauna, which should contribute to more accurate and faster identification of species, including cryptic species, and recognition of relevant vector species. Methods Sampling was carried out in three biogeographical regions of Croatia over six years (2017–2022). The mosquitoes were morphologically identified; molecular identification was based on the standard barcoding region of the mitochondrial COI gene and the nuclear ITS2 region, the latter to identify species within the Anopheles maculipennis complex. The BIN-RESL algorithm assigned the COI sequences to the corresponding BINs (Barcode Index Number clusters) in BOLD, i.e. to putative MOTUs (Molecular Operational Taxonomic Units). The bPTP and ASAP species delimitation methods were applied to the genus datasets in order to verify/confirm the assignment of specimens to specific MOTUs. Results A total of 405 mosquito specimens belonging to six genera and 30 morphospecies were collected and processed. Species delimitation methods assigned the samples to 31 (BIN-RESL), 30 (bPTP) and 28 (ASAP) MOTUs, with most delimited MOTUs matching the morphological identification. Some species of the genera Culex, Aedes and Anopheles were assigned to the same MOTUs, especially species that are difficult to distinguish morphologically and/or represent species complexes. In total, COI barcode sequences for 34 mosquito species and ITS2 sequences for three species of the genus Anopheles were added to the mosquito sequence database for Croatia, including one individual from the Intrudens Group, which represents a new record for the Croatian mosquito fauna. Conclusion We present the results of the first comprehensive study combining morphological and molecular identification of most mosquito species present in Croatia, including several invasive and vector species. With the exception of some closely related species, this study confirmed that DNA barcoding based on COI provides a reliable basis for the identification of mosquito species in Croatia. Graphical Abstract

Since its commencement as part of the Food and Drug Administration’s (FDA) Prescription Drug User Fee Act (PDUFA) V in 2012, patient-focused drug development (PFDD) has become an integral part of the drug development paradigm. FDA encourages the development and use of Patient-Experience Data (PED) as it provides important information on the patients’ needs and perspectives and inform regulatory decision-making. While the FDA is required to fill out a table which includes a list of various types of Patient Experience Data (PED) and if such data was reviewed by FDA as part of a drug application, there is still a need to understand how FDA uses PED in its regulatory decision-making. This article examines whether new policies are needed to ensure the full potential of PFDD.

Purpose: Forward head posture (FHP) is the most common cervical postural fault in the sagittal plane that can cause various issues and conditions. The purpose of this study is to determine the prevalence of FHP among sports-involved children and to examine the differences in craniovertebral angle (CVA) with regard to gender, age, neck pain, and the sports in which they participate. Methods: A cross-sectional study was conducted among 154 sports-involved children (76 males and 78 females), aged 11–14, participating in various sports (basketball, football, volleyball, and athletics). After obtaining signed consent, photography of the sagittal view was performed in a standing position, which was then used to measure the CVA using the Web Plot Digitizer software. A questionnaire was used to gather data about gender, age, sports in which a participant is involved, presence of neck pain, and pain scale. Results: Among the 154 children, 37 (24.03%) exhibited FHP (15 males, 22 females). The mean CVA for the entire sample was 51.30° ± 6.20°. Female participants had a significantly lower CVA (p = .021) compared to male participants. No significant differences were found with regard to age, presence of neck pain, or engagement in a specific sport. Conclusion: FHP is present in sports-involved children, with a higher prevalence among females.

IntroductionCeliac disease is a common autoimmune-like enteropathy caused by an aberrant response to incompletely digested dietary gluten. Gluten immunogenic peptides including the immunodominant 33-mer are thought to be resistant to proteolytic digestion by human gastrointestinal peptidases. We developed a novel enzyme therapy approach to support gluten peptide digestion using a combination of two tandem-acting exopeptidases, AMYNOPEP, that complement the intrinsic enzymatic activity of intestinal brush border enterocytes.MethodsWe evaluated the effects of AMYNOPEP supplementation on 33-mer degradation in vitro and in vivo. In a cross-over clinical study, healthy volunteers with no gastrointestinal disorders were given stable isotope (SI) labelled 33-mer peptides in the presence of varying peptide substrates and caloric loads, with and without AMYNOPEP. 33-mer degradation products (SI-labelled single amino acids) were measured in the blood plasma using LC-MS/MS.ResultsAMYNOPEP achieved rapid, complete amino-to-carboxyl terminal degradation of the 33-mer in vitro, generating single amino acids and dipeptides. In healthy volunteers, AMYNOPEP supplementation significantly increased 33-mer degradation and absorption of SI-labelled amino acids even in the presence of competing substrates. Specifically, we observed a 2.8-fold increase in the Cmax of stable isotope-labelled amino acids in the presence of wheat gluten. The absorption kinetics of labelled amino acids derived from 33-mer digestion with AMYNOPEP closely resembled that of SI-labelled X-Proline dipeptides administered without enzyme supplementation, highlighting the rapid hydrolytic activity of AMYNOPEP on polypeptides.ConclusionsAMYNOPEP achieved complete degradation of the 33-mer into single amino acids and dipeptides in vitro and significantly improved 33-mer degradation kinetics in healthy volunteers, as measured by labelled amino acid detection, warranting further investigation into the potential therapeutic benefits of exopeptidase combinations for patients with gluten-related health disorders including celiac disease.

Introduction: To determine the very early functional as well as oncological outcomes after robot-assisted radical prostatectomy (RARP) and open radical prostatectomy (ORP) at a single institution. Methods: We identified patients who underwent RARP or ORP at our institution between August 2021 and July 2023. The main criterion for surgical technique selection was patient preference. Primary endpoints included anastomosis leakage rate, very early continence rate reported by standardized pad-test, and positive surgical margin rate. Furthermore, we analyzed operation time, hospital stay, postoperative analgesia, and complication rates. Results: In this prospective study, we analyzed data from 222 radical prostatectomies (111 RARP and 111 ORP). There were no significant differences in preoperative age, prostate size, and risk stratification among the groups. Patients who underwent RARP had lower anastomosis leakage rates (8.1% vs. 18.9%) and slightly lower early continence rates (76.6% vs. 78.4%) when compared to patients who underwent ORP. Positive surgical margin rates were similar, and complication rates were also comparable. Operation time was similar for both techniques, but the hospital stay was significantly shorter in the RARP group (6.3 vs. 9.1 days, p=0.03). The ORP group experienced significantly higher opioid administration postoperatively (p

Summary: Background: The inflammatory response in patients with traumatic brain injury (TBI) offers opportunities for stratification and intervention. Previous unselected approaches to immunomodulation in patients with TBI have not improved patient outcomes. Methods: Serum and plasma samples from two prospective, multi-centre observational studies of patients with TBI were used to discover (Collaborative European NeuroTrauma Effectiveness Research [CENTER-TBI], Europe) and validate (Transforming Research and Clinical Knowledge in Traumatic Brain Injury [TRACK-TBI] Pilot, USA) individual variations in the immune response using a multiplex panel of 30 inflammatory mediators. Mediators that were associated with unfavourable outcomes (Glasgow outcome score-extended [GOS-E] ≤ 4) were used for hierarchical clustering to identify patients with similar signatures. Findings: Two clusters were identified in both the discovery and validation cohorts, termed early-inflammatory and pauci-inflammatory. The early-inflammatory phenotype had higher concentrations of interleukin-6 (IL-6), IL-15, and monocyte chemoattractant protein 1 (MCP1). Patients with the early-inflammatory phenotype were older and more likely to have an unfavourable GOS-E at 6 months. There were no differences in the baseline injury severity scores between patients in each phenotype. A combined IL-15 and MCP1 signature identified patients with the early-inflammatory phenotype in both cohorts. Inflammatory processes mediated outcomes in older patients with moderate-severe TBI. Interpretation: Our findings offer a precision medicine approach for future clinical trials of immunomodulation in patients with TBI, by using inflammatory signatures to stratify patients. Funding: CENTER-TBI study was supported by the European Union 7th Framework Programme. TRACK-TBI is supported by the National Institute of Neurological Disorders and Stroke.

Self-service and offering services in public administration is a new concept that should be the basis of its service in the future. Here, we present a concept that should enable not only more efficient service but also the satisfaction of service users. Especially because it would provide them with the option to choose. To implement this concept effectively, it is necessary to implement intelligent public administration that can provide it. For this, we need knowledge bases and intelligent software agents. We will list some of the tools for implementing knowledge bases, and in one tool, we will create an appropriate database. We will use it to present the process of offering services. Here, we will mention the process of forwarding the "Birth Certificate" - a birth certificate that is part of self-service. We will mention offering a "Criminal Record Certificate" and "Title deed" as an example of offering services. These are a unique public administration data warehouse and a necessary means for user identification. There are also necessary legal prerequisites for implementing and using self-service and offering services in intelligent public administration.

Summary: Background: Traumatic brain injury is conventionally categorised as mild, moderate, or severe on the Glasgow Coma Scale (GCS). Recently developed biomarkers can provide more objective and nuanced measures of the extent of brain injury. Methods: Exposure–response relationships were investigated in 2479 patients aged ≥16 enrolled in the Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) prospective observational cohort study. Neurofilament protein-light (NFL), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), and glial fibrillary acidic protein (GFAP) were assayed from serum sampled in the first 24 h; concentrations were divided into quintiles within GCS severity groups. Relationships with the Glasgow Outcome Scale-Extended were examined using modified Poisson regression including age, sex, major extracranial injury, time to sample, and log biomarker concentration as covariates. Findings: Within severity groups there were associations between biomarkers and outcomes after adjustment for covariates: GCS 13–15 and negative CT imaging (relative risks [RRs] from 1.28 to 3.72), GCS 13–15 and positive CT (1.21–2.81), GCS 9–12 (1.16–2.02), GCS 3–8 (1.09–1.94). RRs were associated with clinically important differences in expectations of prognosis. In patients with GCS 3 (RRs 1.51–1.80) percentages of unfavourable outcome were 37–51% in the lowest quintiles of biomarker levels and reached 90–94% in the highest quintiles. Similarly, for GCS 15 (RRs 1.83–3.79), the percentages were 2–4% and 19–28% in the lowest and highest biomarker quintiles, respectively. Interpretation: Conventional TBI severity classification is inadequate and underestimates heterogeneity of brain injury and associated outcomes. The adoption of circulating biomarkers can add to clinical assessment of injury severity. Funding: European Union 7th Framework program (EC grant 602150), Hannelore Kohl Stiftung, One Mind, Integra LifeSciences, Neuro-Trauma Sciences, NIHR Rosetrees Trust.

Variable frequent drought periods in Vojvodina region (the southeastern part of the Pannonian Plain, Serbia), severely limit maize (Zea mays L.) production under rainfed conditions. A four-year field experiment was conducted to investigate the effects of sprinkler deficit irrigation and plant density on yield and water use efficiency of maize grown on silty clay loam soil under temperate continental climate. The experiment included fully irrigated crop (I1), three deficit irrigation treatments (I2, I3 and I4 corresponding to 80, 50 and 40 % of crop evapontranspiration, respectively) and non-irrigated treatment (I0), and three plant densities (LPD: 54,900 plants ha–1; MPD: 64,900 plants ha–1; HPD: 75,200 plants ha–1) in four replicates. The results showed that grain yield, CWP (crop water productivity) and IWP (irrigation water productivity) varied significantly with irrigation amounts, plant densities and seasons. The irrigation rates and plant density interact significantly. MPD and HPD differed significantly from LPD in almost all seasons. With increasing irrigation and plant density, yield and CWP showed an increasing trend. The relative values of IWP increased with the rise of plant density and decreased with the amount of irrigation. The highest four-year average yield (15.03 t ha–1) was obtained in the I1-HPD treatment, while the lowest (9.30 t ha–1) was obtained in the I0-HPD treatment. The highest average values of CWP and IWP were recorded at I3-HPD or/and I2-HPD. The lowest CWP and IWP values were determined for I4-HPD and I4-MPD, respectively. In the pedo-climatic conditions of Vojvodina and similar regions, we recommend growing maize under the I1-HPDtreatment to achieve high yields. Under the water shortage conditions, the application of I2-HPD treatment is a favorable strategy saving 37 % of irrigation water, while reducing maize grain yield by ∼10 %.

Abstract Quantum technologic and spintronic applications require reliable material platforms that enable significant and long‐living spin polarization of excitations, the ability to manipulate it optically in external fields, and the possibility to implement quantum correlations between spins, i.e., entanglement. Here it is demonstrated that these conditions are met in bulk crystals of lead halide perovskites. A giant optical orientation of 85% of excitons, approaching the ultimate limit of unity, in FA0.9Cs0.1PbI2.8Br0.2 crystals is reported. The exciton spin orientation is maintained during the exciton lifetime of 55 ps resulting in high circular polarization of the exciton emission. The optical orientation is robust to detuning of the excitation energy up to 0.3 eV above the exciton resonance and remains larger than 20% up to detunings of 0.9 eV. It evidences pure chiral selection rules and suppressed spin relaxation of electrons and holes, even with large kinetic energies. The exciton and electron–hole recombinations are distinguished by means of the spin dynamics detected via coherent spin quantum beats in magnetic field. Further, electron–hole spin correlations are demonstrated through linear polarization beats after circularly polarized excitation. These findings are supported by atomistic calculations. All‐in‐all, the results establish lead halide perovskite semiconductors as suitable platform for quantum technologies.

The paper presents research on measuring and the possibility of prediction of noise levels on the left and right sides of the operator within the cabin of an agricultural tractor when moving across various agrotechnical surfaces, considering movement velocity and tire pressures while employing machine learning techniques. Noise level measurements were conducted on a LANDINI POWERFARM 100 type tractor, and aligned with standards (HRN ISO 5008, HRN ISO 6396 and HRN ISO 5131). The obtained noise values were divided into two data sets (left and right set) and processed using multiple linear regression (mlr) and three machine learning methods (gradient boosting machine (gbm); support vector machine using radial basis function kernel (svmRadial); monotone multi-layer perceptron neural network (monmlp)). The most accurate method, considering surfaces, from the left side data set—(R2 0.515–0.955); (RMSE 0.302–0.704); (MAE 0.225–0.488)—and the right side—(R2 0.555–0.955); (RMSE 0.180–0.969); (MAE 0.139–0.644)—was monmlp predominantly, and to a lesser extent svmRadial. On analyzing the total data sets from the left and right sides regarding surfaces, gbm emerged as the most accurate method. The application of machine learning methods demonstrated data accuracy, yet in future research, measurements on certain surfaces may need to be repeated multiple times potentially to improve accuracy further.

Introduction. Biliary obstruction is a common manifestation of biliopancreatic malignancies, and its relief is an essential part of the treatment algorithm. Currently, there are three techniques to manage malignant biliary obstruction—endoscopic retrograde cholangiopancreatography (ERCP), percutaneous transhepatic biliary drainage (PTBD), and endoscopic ultrasound-guided biliary drainage (EUS-BD). ERCP has been adopted as a first-line treatment modality but EUS-BD is gradually emerging as a viable alternative. The aim of the current article is to assess the clinical outcomes of the three nonsurgical biliary drainage procedures. Materials and methods. A total of 102 consecutive patients with unresectable biliopancreatic malignancy inducing biliary obstruction and subjected to palliative treatment by means of ERCP, EUS-BD, or PTBD were retrospectively included in the study. Results. No difference in clinical and technical success of the procedures was found: ERCP—97.2% technical; 88.9% clinical; PTBD—94.4% technical, 72.2% clinical; EUS-BD—90% technical; 83.3% clinical. Adverse events (AEs) and reinterventions were significantly more common in PTBD (38.9% and 52.8%) and ERCP (27.9% and 25%) compared to EUS-BD (10% and 3.3%). Total duration of hospital stay and number of hospitalizations were lower in the EUS-BD compared to PTBD and ERCP groups. Conclusions. In the presence of adequate expertise, EUS-BD may be superior to PTBD and ERCP in achieving and sustaining biliary drainage in the setting of unresectable malignancy.

Abstract Background Aerobic anoxygenic phototrophs are metabolically highly active, diverse and widespread polyphyletic members of bacterioplankton whose photoheterotrophic capabilities shifted the paradigm about simplicity of the microbial food chain. Despite their considerable contribution to the transformation of organic matter in marine environments, relatively little is still known about their community structure and ecology at fine-scale taxonomic resolution. Up to date, there is no comprehensive (i.e. qualitative and quantitative) analysis of their community composition in the Adriatic Sea. Results Analysis was based on pufM gene metabarcoding and quantitative FISH-IR approach with the use of artificial neural network. Significant seasonality was observed with regards to absolute abundances (maximum average abundances in spring 2.136 ± 0.081 × 104 cells mL−1, minimum in summer 0.86 × 104 cells mL−1), FISH-IR groups (Roseobacter clade prevalent in autumn, other Alpha- and Gammaproteobacteria in summer) and pufM sequencing data agglomerated at genus-level. FISH-IR results revealed heterogeneity with the highest average relative contribution of AAPs assigned to Roseobacter clade (37.66%), followed by Gammaproteobacteria (35.25%) and general Alphaproteobacteria (31.15%). Community composition obtained via pufM sequencing was dominated by Gammaproteobacteria clade NOR5/OM60, specifically genus Luminiphilus, with numerous rare genera present in relative abundances below 1%. The use of artificial neural network connected this community to biotic (heterotrophic bacteria, HNA and LNA bacteria, Synechococcus, Prochlorococcus, picoeukaryotes, heterotrophic nanoflagellates, bacterial production) and abiotic environmental factors (temperature, salinity, chlorophyll a and nitrate, nitrite, ammonia, total nitrogen, silicate, and orthophosphate concentration). A type of neural network, neural gas analysis at order-, genus- and ASV-level, resulted in five distinct best matching units (representing particular environments) and revealed that high diversity was generally independent of temperature, salinity, and trophic status of the environment, indicating a potentially dissimilar behaviour of aerobic anoxygenic phototrophs compared to the general bacterioplankton. Conclusion This research represents the first comprehensive analysis of aerobic anoxygenic phototrophs in the Adriatic Sea on a trophic gradient during a year-round period. This study is also one of the first reports of their genus-level ecology linked to biotic and abiotic environmental factors revealed by unsupervised neural network algorithm, paving the way for further research of substantial contribution of this important bacterial functional group to marine ecosystems.

In Medvednica Nature Park, near Croatia's capital Zagreb, urbanisation significantly impacts the fauna. Comprehensive field research has never been conducted in this area, despite the presence of diverse microhabitats and the discovery of several rare species previously unknown in the Croatian fauna. This study provides the Park with first insight into the genetic and morphological diversity of stoneflies, one of the most endangered groups of organisms. Phylogenetic reconstructions and species delineation methods revealed intraspecific haplotype variation in most species (e.g. Brachyptera seticornis, Isoperla grammatica and Leuctra braueri), except for Leuctra prima. Additionally, our study has identified isolated populations that merit further in-depth investigation concerning morphology, genetics and ecology.

Abstract Ewing's sarcoma (ES) is the second most common bone tumor in children and adolescents and is highly malignant. Although the new chemotherapy has significantly improved the survival rate for ES from about 10 to 75%, the survival rate for metastatic tumors remains around 30%. This treatment is often associated with various side effects that contribute to the suffering of the patients. Cold physical plasma (CPP), whether used alone or in combination with current chemotherapy, is considered a promising adjunctive tool in cancer treatment. This study aims to investigate the synergistic effects of CPP in combination with cytostatic chemotherapeutic agents that are not part of current ES therapy. Two different ES cell lines, RD-ES and A673, were treated with the determined IC20 concentrations of the chemotherapeutic agents cisplatin and methotrexate (MTX) in combination with CPP. The effects on population doubling, cell viability, and apoptotic processes within these cell lines were assessed. This combination therapy has led to a reduction of population doubling and cell viability, as well as an increase in apoptotic activity in cells compared to CPP monotherapy. The results of this study provide evidence that combining CPP with non-common chemotherapy drugs such as MTX and CIS in the treatment of ES enhances the anticancer effects of these drugs. These findings open up new possibilities for the effective use of these drugs against ES.

From the era of Grammar-Translation Method to the onset of Communicative Language Teaching, the role of translation in language learning has completely changed. Nowadays, the use of translation activities in the language learning classroom remains a contentious issue, namely, there are arguments for and against their use. Given that attitudes are one of the factors that affect the way students learn and the level of proficiency they achieve, the aim of this quantitative study is to find out what the attitudes of higher education students in Croatia are towards the use of translation in learning languages for specific purposes. The responses were collected via a questionnaire, which was completed by 618 students who were enrolled in eight different LSP courses, i.e. medical, maritime, business, agriculture, tourism, safety, IT and transport. The participants come from five institutions of higher education in two Croatian towns (Zagreb and Rijeka). The majority of the respondents (86.4%) claim they use translation in their LSP classroom, but the frequency in using translation varies greatly. On the other hand, translation is used in assessment less frequently, 37.4% of the participants state they have encountered translation in tests. While they believe that other more communicative methods are more beneficial for their language learning, and they only partially enjoy doing translation activities, most of them are sure that translating both from their L1 into L2 and L2 into L1 is very useful for their language learning, especially when it comes to learning new vocabulary. A statistical difference occurred between genders; namely, male students seem to be more positive towards translation. Also, statistical differences were confirmed among students of different study programs in relation to their attitudes towards enjoyment, usefulness and demand of translation activities. Future research should focus on investigating how translation activities are implemented and account for gender and study program differences.

Fry of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792), was subjected to one-hour and four hours immersion in Alizarin red S (ARS) bath 150 mg · L–1. The experiment involved seven age groups (40, 50, 60, 70, 80, 90, and 100 days) and was conducted to estimate the minimal age of salmonids for mass marking with ARS bath enabling subsequent effective, simple field nonlethal identification, based on fin rays checking. The fish were examined at the ages of 200 and 300 days. The results showed a high retention level of ARS traces in caudal fin rays ensured satisfactory visibility and quick detection. A success rate of marking detection was >90% at 200 and 300 days of age and the fish were immersed in ARS solution from 60 days of age (685°D). This treatment provided better results in fish bathed for four hours. Recognition of marks using a laser pointer and protective glasses was successful even in fish, with a 3.5–5.0 times length increase compared to the marking time. The results indicate a high potential for ARS marking and its field identification for juvenile salmonids, which can significantly expand the possibilities of field experiments.

A method for gamma/neutron event classification based on frequency-domain analysis for mixed radiation environments is proposed. In contrast to the traditional charge comparison method for pulse-shape discrimination, which requires baseline removal and pulse alignment, our method does not need any preprocessing of the digitized data, apart from removing saturated traces in sporadic pile-up scenarios. It also features the identification of neutron events in the detector’s full energy range with a single device, from thermal neutrons to fast neutrons, including low-energy pulses, and still provides a superior figure-of-merit for classification.The proposed frequency-domain analysis consists of computing the fast Fourier transform of a triggered trace and integrating it through a simplified version of the transform magnitude components that distinguish the neutron features from those of the gamma photons. Owing to this simplification, the proposed method may be easily ported to a real-time embedded deployment based on Field-Programmable Gate Arrays or Digital Signal Processors. We target an off-the-shelf detector based on a small CLYC (Cs2LiYCl6:Ce) crystal coupled to a silicon photomultiplier with an integrated bias and preamplifier, aiming at lightweight embedded mixed radiation monitors and dosimeter applications.

The paper shows the integration of the theoretical and practical aspects of designing an ERM software tool. The basic idea of the designed ERM is conceived in the form of an algorithm using the integration of ISO 3100, the COSO framework, the risk matrix designed according to the risk appetite of the observed company, quantitative and qualitative models for risk assessment, and the generation of consequences and mitigating measures for each identified risk. Methodologies for risk assessment in the presented ERM include the following: For business risks, questionnaires were generated for different business areas (from knowledge bases) and the assessment was conducted according to risk matrices. Workplace risk is assessed according to the Kinney method, while technical risks are assessed according to the API 580, 581 standard. Software is created to outbalance problems of all types of risk that may arise regardless of the complexity of the business and the risk itself. The algorithm, model and software were developed and successfully tested in two Serbian companies.

Background/Objectives. Our study aimed to determine the development of antibiotic resistance during the peri-pandemic period in a regional secondary care hospital using an interrupted time series analysis. Methods. We analyzed data from seven years, accounting for 441,149 patient days. The incidence density of multidrug-resistant bacteria (MDR) burden and infection was reported per 1000 patient days. Results. During the COVID-19 period, a significant increase in the mean incidence density of the total MDR burden from 4.93 to 5.81 per 1000 patient days was observed (p = 0.007). On the other hand, the mean incidence density of MDR infections decreased from 1.61 to 1.29 per 1000 patient days (p = 0.019). Using the interrupted time series analysis, the same trends were observed, namely the overall increasing trend in MDR burden and the overall decreasing trend in MDR infections. This divergent trend is mainly due to similar trends in several Gram-negative MDR, namely ESBL-EC, ESBL-KP and CRE. Conclusions. Due to the increasing burden of MDR, it is necessary to strengthen AMR surveillance. In addition, strict infection prevention and control measures, and antimicrobial stewardship programs continue to be important components in the fight against resistant bacteria.

Breast cancer is the most prevalent cancer worldwide, affecting both low- and middle-income countries, with a growing number of cases. In 2024, about 310,720 women in the U.S. are projected to receive an invasive breast cancer diagnosis, alongside 56,500 cases of ductal carcinoma in situ (DCIS). Breast cancer occurs in every country of the world in women at any age after puberty but with increasing rates in later life. About 65% of women with the BRCA1 and 45% with the BRCA2 gene variants develop breast cancer by age 70. While these genes account for 5% of breast cancers, their prevalence is higher in certain populations. Advances in early detection, personalised medicine, and AI-driven diagnostics are improving outcomes by enabling a more precise analysis, reducing recurrence, and minimising treatment side effects. Our paper aims to explore the vast applications of artificial intelligence within the diagnosis and treatment of breast cancer and how these advancements can contribute to elevating patient care as well as discussing the potential drawbacks of such integrations into modern medicine. We structured our paper as a non-systematic review and utilised Google Scholar and PubMed databases to review literature regarding the incorporation of AI in the diagnosis and treatment of non-palpable breast masses. AI is revolutionising breast cancer management by enhancing imaging, pathology, and personalised treatment. In imaging, AI can improve the detection of cancer in mammography, MRIs, and ultrasounds, rivalling expert radiologists in accuracy. In pathology, AI enhances biomarker detection, improving HER2 and Ki67 assessments. Personalised medicine benefits from AI’s predictive power, aiding risk stratification and treatment response. AI also shows promise in triple-negative breast cancer management, offering better prognosis and subtype classification. However, challenges include data variability, ethical concerns, and real-world validation. Despite limitations, AI integration offers significant potential in improving breast cancer diagnosis, prognosis, and treatment outcomes.