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Fusarium graminearumcauses Fusarium head blight (FHB) in wheat, resulting in severe yield losses. Further, it produces mycotoxins, which can have adverse effects on human health. This study aimed to assess the biocontrol efficacy of Metarhizium anisopliaeagainst F. graminearumcausing FHB in wheat. M. anisopliaesignificantly inhibited F. graminearummycelial growth in the dual culture test, resulting in hyphal deformation at the site of its hyphal tips. Similar effects were observed with a 1% concentration of broth filtrates from M. anisopliaeon mycelial growth and hyphal morphology of F. graminearum.However, the same was not observed for its volatiles. Furthermore, a 1% concentration of broth filtrates impeded conidial germination of F. graminearum. The colonisation ability of M. anisopliaeon wheat roots was validated through eGFP-labelled M. anisopliaestrain Ma-eGFP. The positive effects of M. anisopliaeon seedling growth and single-spike weight of wheat were recorded. However, no noticeable effect on the 1000-seed weight of wheat was observed. Results from field experiments revealed that treating wheat seeds with the seed coating agent of M. anisopliaeand spraying wheat heads with M. anisopliaeconidia suspension resulted in a significant control efficacy of 38.6% and 40.1% at the early flowering period of wheat, respectively. These findings suggest that M. anisopliaeis a promising biocontrol agent against F. graminearumcausing FHB in wheat, and may fight against FHB by producing fungistatic secondary metabolites, enhancing wheat growth, and eliciting wheat defence responses.