1. Cytotoxic Potential and Molecular Characterization of Fungal Endophytes from Selected High Value Medicinal Plants of the Kashmir Valley - India.
- Author
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Dar RA, Qazi PH, Saba I, Rather SA, Wani ZA, Qazi AK, Shiekh AA, Manzoor A, Hamid A, and Modae DM
- Subjects
- A549 Cells, Animals, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Caco-2 Cells, Cell Line, Tumor, Humans, India, MCF-7 Cells, Mice, Phylogeny, Endophytes chemistry, Fungi chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Plant Extracts therapeutic use, Plants, Medicinal chemistry
- Abstract
The present study explores the fungal endophytes from selected high value medicinal plants to check their activities at in-vitro and in-vivo level. The in-vitro cytotoxicity of selected endophytes revealed potent growth inhibition against human cancer cell lines of leukemia (THP-1), lung (A549), prostate (PC-3), colon (Caco-2), neuroblastoma (IMR-32) and breast (MCF-7) at a concentration of 100 µg/ml. Among them the endophytic strains I. e., IIIM2, IIIM3, IIIM7 and IIIM8 showed most significant growth inhibition against colon (Caco-2), prostate (PC-3), lung (A549) and leukemia (THP-1) cancer cell lines. At the in-vivo level maximum (58.95%) tumor growth inhibition was documented with the extract of IIIM2 against Ehrlich Ascites Carcinoma mouse modal. All the potent fungal endophytic strains were characterized using ITS 4 and ITS 5 region sequencing and phylogenetic analysis was ascertained among them. This paper confirms the 2 elite endophytic fungal strains, IIIM2 and IIIM8, have the potential to act as a source of new anticancer compounds., (© Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2016
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