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227 results on '"Mogas, T."'

4. 51

Author

Díaz-Muñoz, J., primary, Martínez-Rodero, I., additional, Higgins, A., additional, Mogas, T., additional, and García-Martínez, T., additional

Published
2022
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10. A shorter equilibration period in the VitTrans in-straw bovine embryo vitrification method improves post-warming outcomes

Author

Meritxell Vendrell-Flotats, Mendebil X, Iris Martínez-Rodero, Olegario-Hidalgo C, Azcarate S, Tania García-Martínez, Mogas T, Manel Lopez-Bejar, Esmoris J, Ordóñez-León Ea, and Yeste M

Subjects
Animal science, Period (gene), embryonic structures, Vitrification, Bovine embryo, Straw, Biology
Abstract

Background VitTrans is a device that enables the vitrification and warming/dilution of in vitro produced bovine embryos followed by their direct transfer to recipient females in field conditions. This study sought to improve the VitTrans method by comparing two equilibration times: short (SE: 3 min) and long (LE: 12 min). Outcome measures recorded in vitrified D7 and D8 expanded blastocysts were survival and hatching rates, differential cell counts, apoptosis rate and gene expression. Results While survival rates at 3 h and 24 h post-warming were reduced (P

Published
2020

20. Spindle configuration of in vitro matured bovine oocytes vitrified and warmed in media supplemented with a biopolymer produced by an Antarctic bacterium

Author

Arcarons, N., Vendrell, M., Yeste, M., Mercadé Gil, M. Elena, and Mogas, T.

Subjects
Vitrification, Chromosomes, Vitrificació, Cromosomes
Abstract

Biological molecules isolated from organisms that live under subfreezing conditions could be used to protect oocytes from cryoinjuries suffered during cryopreservation. Bacterial exopolysaccharides (EPS) constitute a common class of molecules that interact with ice in nature either by triggering ice nucleation or by inhibition of ice nucleation and growth. The aim of this work was to evaluate the spindle configuration of in vitro matured bovine oocytes vitrified/warmed in media supplemented with exopolysaccharide (M1) produced by Pseudomonas sp ID1 (Carrión et al., Carbohydr Polym 117:1028. 2015). After 22 h of in vitro maturation, a total of 546 oocytes form prepubertal (3 replicates) and 405 oocytes from adult cows (4 replicates) were vitrified/warmed in media supplemented with various concentrations of EPS M1 (0, 0.001, 0.01, 0.1 and 1 mg/ml). After warming, oocytes were allowed to recover for 2 additional hours in IVM medium. Fresh, non-vitrified oocytes were used as a control. At 24 h of IVM, oocytes from all treatments were fixed and immunostained with the Alexa-fluor 488 antibody and DAPI. Microtubule and chromosome distribution was analyzed by immunocytochemistry under a fluorescent microscope. ANOVA was performed to analyze differences in meiotic spindle configuration (P < 0.05). When cow oocytes were vitrified, similar percentages of normal spindle configuration were observed when compared to fresh control oocytes, except for the 0.1 mg/ml EPS M1 group that showed significantly lower rates compared to the fresh control group. Significantly higher rates of prepubertal oocytes exhibiting a normal spindle configuration were recorded in the non-vitrified group compared to all vitrified/warmed groups, regardless of the EPS M1 supplementation. However, the addition of EPS M1 to the vitrification/warming media decreased the ratio of decondensation or absence of chromosomes and microtubules in prepubertal oocytes. Although percentages of normal spindle configuration after vitrification were lower for prepubertal than for cow oocytes, no significant differences were observed when oocytes were vitrified with 0.001, 0.1 and 1 mg/ml EPS M1. In conclusion, supplementation with EPS M1 concentrations during vitrification and warming did not induce adverse changes in the spindle of bovine oocytes, regardless of the concentration used. Although a more severe damage on spindle configuration could be observed after vitrification of prepubertal oocytes, EPS supplementation during vitrification and warming seems to have a greater benefit during vitrification of prepubertal than adult bovine oocytes. Further experiments are required to investigate if in vitro-matured oocytes vitrified/warmed in presence EPS M1 can improve their development competence after being vitrified/warmed. This study was supported by the Spanish Ministry of Science and Innovation (Project AGL2016-79802-P and grant CTQ2014-59632-R).

Published
2017

29. Decrease in bovine in vitro embryo production efficiency during winter season in a warm-summer Mediterranean climate.

Author

Cheuquemán, C., Loren, P., Arias, M., Risopatrón, J., Felmer, R., Álvarez, J., Mogas, T., and Sánchez, R.

Subjects
BOS, EMBRYOS, MEDITERRANEAN climate, BLASTOCYST, OVUM
Abstract

Retrospective analysis of monthly embryo production from December 2011 to May 2015 and its correlation with meteorological data in our geographic zone was made. We had observed that in certain time of the year, in vitro blastocyst production decreases. Accordingly, was examined the association between blastocyst production and climatological parameters. Cleavage rates correlate positively with blastocyst rates ( p < .05). Significant differences in cleavage rates between autumn and summer (79.8%; 71.5%), and between winter and autumn (71.8%; 79.8%), were found. Blastocyst production had lower efficiency in June (9 ± 12%) and July (4.9 ± 5.7%), which coincides with winter season. In contrast, higher embryo production was obtained in February (22.2 ± 9.7%), March (22.9 ± 14%) and September (25.2 ± 6.6%), which coincides with autumn and spring season. Similarly, embryo production correlates with meteorological parameters: blastocyst production positively correlates with sunshine hours, maximum temperature and average temperature. Similarly, blastocyst production inversely correlates with total precipitation and days >1 mm precipitation ( p < .05). There is a significant decrease in bovine in vitro embryo production efficiency during winter season in our warm-summer Mediterranean climate zone. It remains to be investigated the direct effect of environmental factors on oocyte quality and its impact on in vitro production efficiency. [ABSTRACT FROM AUTHOR]

Published
2017
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44. Coenzyme Q10 supplementation during in vitro maturation of bovine oocytes ( Bos taurus) helps to preserve oocyte integrity after vitrification.

Author

Ruiz-Conca, M, Vendrell, M, Sabés-Alsina, M, Mogas, T, and Lopez-Bejar, M

Subjects
BLASTOCYST, FERTILIZATION in vitro, DEVELOPMENTAL biology, EMBRYOS, VETERINARY medicine
Abstract

Contents Oocyte vitrification causes less cell stress than slow cooling, but cytoskeletal and spindle alterations may occur affecting the oocyte competence. In vitro maturation ( IVM) supplementation with different antioxidant molecules has been performed to attenuate this harmful stress. Coenzyme Q10 (CoQ10) supplementation has previously shown to have positive effects in bovine and mouse in vitro embryo development. The aim of this study was to evaluate the effects of CoQ10 during bovine oocyte IVM and vitrification. Cumulus-oocyte complexes ( COCs) ( n = 311) were cultured under standard maturation conditions with 0 μM (control), 25 μM and 50 μM CoQ10 supplementation. After 22 hr, a cohort of 170 oocytes both from the control and from CoQ10-supplemented groups were vitrified, warmed and returned to incubation until 24 hr of maturation, while the rest of the oocytes ( n = 141) remained fresh. Then, oocyte survival was assessed morphologically by stereomicroscopy. Oocytes from all groups were then fixed and stained for assessing cortical granules ( CG) migration and nuclear stage. High rates of oocyte MII progression and appropriate CG migration as a continuous layer beneath the plasma membrane were obtained both in control and in CoQ10 groups. Results showed that although vitrification has great impact in survival of IVM bovine oocytes, 50 μM CoQ10 supplementation significantly improved oocyte survival ( p = .045) and reduced the premature CG exocytosis, helping to preserve the CG migration pattern (31.3% control vs. 54.5% in 50 μM CoQ10; p = .039), attenuating the negative effects of vitrification. [ABSTRACT FROM AUTHOR]

Published
2017
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