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Diabetes Mellitus is a chronic metabolic disease presented with chronic hyperglycemia, which causes abnormalities in the endothelial cells, proinflammatory state, altered platelet function & plasma coagulation factors that lead to a hypercoagulable state & thrombotic incidents. D-dimer is an indirect marker of thrombotic activity. C-reactive protein (CRP) is an important risk factor for systemic inflammation, so the present study aimed to determine the relationship between CRP, D-dimer in correlation with HbA1C in patients with Diabetes Mellitus. The present study is undertaken to evaluate the study of D-dimer, C-reactive protein in correlation with HbA1C levels in Type 2 Diabetes Mellitus. : A total of one hundred & twenty subjects aged 30-70 years were included. The patients were selected from those who attended the medical OPD at Gandhi Medical College & hospital, Secunderabad, & their samples are analyzed by Beckmann Coulter Fully Automatic Analyzer AU5800. CRP is estimated by rate turbidimetry method & D-dimer is estimated by rate turbidimetry method. HbA1C by HPLC in Bio-rad-D-10: The prospective of elevated D-dimer, CRP levels are in correlation with increased HbA1C levels. So screening of inflammatory & hemostatic markers must be done in patients with uncontrolled Diabetes Mellitus.

Diabetes is a metabolic disease which has hyperglycaemia resulting from defects in insulin secretion or action. HbA1C is one of the most reliable test used for estimation of glycaemic index. Diabetes is often accompanied by undiagnosed dyslipidaemia, characterized by increased triglyceride level, decreased high-density lipoprotein levels and increase in low-density lipoprotein (LDL) particles. Diabetes is a significant risk factor for atherosclerotic cardiovascular disease (ASCVD). The purpose of this study was to correlate lipid parameters with different levels of HbA1c to assess dyslipidaemia with diabetic status. An observational study was conducted in biochemistry laboratory at Gandhi Hospital, Telangana.After informed consent, 5ml of venous blood sample was collected in plain tube and EDTA tube from diabetic and apparently non diabetic patients. Estimation of serum triglycerides was done by glycerol phosphate oxidase peroxidase, serum cholesterol by cholesterol oxidase method, serum HDL by direct clearance method in Beckmann Coulter AU 500 and HbA1c was estimated by high performance liquid chromatography in Bio-Rad D10.A total of 90 samples were divided into 3 groups according to their HbA1c results. Group 1 with HbA1c less than 5.6, Group 2 with HbA1c 5.6-7 and Group 3 with HbA1c more than 7.The present study showed no variation in the lipid profile in Group1 with HbA1c < 5.6. In Group 2 with HbA1c between 5.6-7, there was significant increase in LDL cholesterol. In Group 3 with HbA1c >7 there was marked increase in LDL, serum triglyceride. Hence we can conclude that dyslipidemia is increased in poor glycemic control and evaluation of HbA1c may also be used as a predictor of dyslipidaemia in diabetics.

Background: Coagulation tests are widely applied in clinical practice, among which PT and aPTT are the most commonly done. Among many preanalytical conditions –time, temperature and storage conditions are few of the factors affecting the results of samples. This study aims to compare PT and aPTT values in primary tubes and sample aliquots after 4 hours duration with the initial value. Materials & Methods : An observational study was done at Central Diagnostic Laboratory, Gandhi Hospital, Secunderabad, T.S. Samples were collected in citrated tubes, centrifuged, PT and aPTT were processed with plasma of primary tubes. Sufcient quantity of plasma was aliquoted immediately from primary tubes and kept at room temperature for 4 hours after which the plasma of primary tubes and aliquots were re-analyzed separately and values were compared. Results: Pearson correlation was performed to show the relation. PT and aPTT values obtained in the primary tube and aliquot were correlating with the initial value (PT-r =0.833,0.831 respectively) and (aPTT-r =0.97,0.87 respectively) and also with each other (PT-r =0.99) and (aPTT-r =0.90) respectively. There is no statistical signicant difference in PT of primary tube and aliquots when compared to initial PT (p>0.01). Incase of APTT there is statistically signicant difference in primary tube and aliquot when compared to initial value (p0.01). Conclusion: The present study shows no difference of PT values in aliquots and primary tube when compared to initial value, whereas APTT values differ from initial values in samples stored for more than 4 hours in primary tube and aliquot.