Introduction: Needlestick injuries (NSIs) pose a significant occupational hazard to healthcare workers (HCWs), with potential risks of exposure to bloodborne pathogens. The development of effective training modules is crucial to addressing NSI prevention and enhancing HCWs' knowledge and risk perception. This study aims to develop and validate the Needlestick Injury Prevention Module (N-SIP) using the ADDIE model (Florida State University, FL), which stands for Analysis, Design, Development, Implementation, and Evaluation, to improve NSI-related knowledge and risk perception among House Officers (HOs) in healthcare settings., Methods: The study utilized approaches comprising literature review, module development using the ADDIE model, content validation by experts, and face validation among HOs. The N-SIP module addressed various aspects of NSI prevention, including background information, bloodborne viral infections, infection prevention practices, and occupational safety. The evaluation involved content validation by expert panels and face validation by HOs., Results: The content validity of the N-SIP module was rigorously evaluated through expert review and validation by subject matter experts and HOs. The experts' feedback ensured the quality, relevance, and comprehensiveness of the module's instructional materials. Furthermore, face validity was assessed among HOs to ensure the module's clarity, appropriateness, and perceived effectiveness in addressing NSI prevention. The positive response from HOs indicated favorable perceptions of the module's content and instructional design, affirming its potential to effectively enhance perceptions related to NSI prevention among HCWs., Conclusion: The development and evaluation of the N-SIP represent a significant advancement in addressing NSIs among HCWs. Through a structured approach informed by the ADDIE model, the N-SIP module offers a comprehensive and tailored learning experience aimed at enhancing NSI-related knowledge and risk perception among HOs. The study findings underscore the importance of effective training interventions in promoting a culture of safety and reducing occupational hazards in healthcare settings., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Human Research and Ethics Committee, Universiti Sains Malaysia and Ethical Committee from the National Institute of Health, Ministry of Health Malaysia issued approval USM/JEPeM/KK/23010096 and NMRR ID-23-00281-VRX (IIR). Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Mohd Kutubudin et al.)