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2. Combining epidemiological data and whole genome sequencing to understand SARS-CoV-2 transmission dynamics in a large tertiary care hospital during the first COVID-19 wave in The Netherlands focusing on healthcare workers

Author

Haanappel, Cynthia P., Oude Munnink, Bas B., Sikkema, Reina S., Voor in ’t holt, Anne F., de Jager, Herbert, de Boever, Rieneke, Koene, Heidy H. H. T., Boter, Marjan, Chestakova, Irina V., van der Linden, Anne, Molenkamp, Richard, Osbak, Kara K., Arcilla, Maris S., Vos, Margreet C., Koopmans, Marion P. G., and Severin, Juliëtte A.

Published
2023
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3. Rise and fall of SARS-CoV-2 variants in Rotterdam: Comparison of wastewater and clinical surveillance

Author

Izquierdo-Lara, Ray W., Heijnen, Leo, Oude Munnink, Bas B., Schapendonk, Claudia M.E., Elsinga, Goffe, Langeveld, Jeroen, Post, Johan, Prasad, Divyae K., Carrizosa, Christian, Been, Frederic, van Beek, Janko, Schilperoort, Remy, Vriend, Rianne, Fanoy, Ewout, de Schepper, Evelien I.T., Sikkema, Reina S., Molenkamp, Richard, Aarestrup, Frank M., Medema, Gertjan, Koopmans, Marion P.G., and de Graaf, Miranda

Published
2023
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5. Head-to-head comparison of the accuracy of saliva and nasal rapid antigen SARS-CoV-2 self-testing: cross-sectional study

Author

Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J. J. M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R. S., Han, Wanda G. H., Kluytmans, Jan A. J. W., van den Hof, Susan, van de Wijgert, Janneke H. H. M., and Moons, Karel G. M.

Published
2022
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6. Detection of SARS-CoV-2 infection in the general population by three prevailing rapid antigen tests: cross-sectional diagnostic accuracy study

Author

Venekamp, Roderick P., Veldhuijzen, Irene K., Moons, Karel G. M., van den Bijllaardt, Wouter, Pas, Suzan D., Lodder, Esther B., Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, dos Santos, Claudy Oliveira, Debast, Sylvia B., Bruins, Marjan J., Polad, Khaled, Nagel-Imming, Carla R. S., Han, Wanda G. H., van de Wijgert, Janneke H. H. M., van den Hof, Susan, and Schuit, Ewoud

Published
2022
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9. Epidemiological and clinical insights into the enterovirus D68 upsurge in Europe 2021/22 and the emergence of novel B3-derived lineages, ENPEN multicentre study

Author

Simoes, Margarida Pires, primary, Hodcroft, Emma B, additional, Simmonds, Peter, additional, Albert, Jan, additional, Alidjinou, Enagon K, additional, Ambert-Balay, Katia, additional, Andrés, Cristina, additional, Antón, Andrés, additional, Auvray, Christelle, additional, Bailly, Jean-Luc, additional, Baldanti, Fausto, additional, Bastings, Capser, additional, Beard, Stuart, additional, Pereira, Carla Berengua, additional, Berginc, Natasa, additional, Bloemen, Mandy, additional, Blomqvist, Soile, additional, Bosma, Froukje, additional, Böttcher, Sindy, additional, Bubba, Laura, additional, Buderus, Stafan, additional, Cabrerizo, Maria, additional, Calvo, Christina, additional, Celma, Christina, additional, Ceriotti, Ferruccio, additional, Clark, Gemma, additional, Costa, Inës, additional, Coste-Burel, Marianne, additional, Couderé, Karen, additional, Cremer, Jeroen, additional, del Cuerpo Casas, Margarita, additional, Daehne, Theo, additional, de Beer, Jessica, additional, de Ceano-Vivas, Maria, additional, De Gascun, Cillian, additional, de Rougemont, Alexis, additional, Dean, Johnathan, additional, Dembinski, Jennifer L, additional, Diedrich, Sabine, additional, Diez-Domingo, Javier, additional, Dillner, Lena, additional, Dorenberg, Dagny H, additional, Ducancelle, Alexandra, additional, Dudman, Susanne, additional, Dyrdak, Robert, additional, Eis-Huebinger, Anna-Maria, additional, Falces-Romero, Iker, additional, Farkas, Agnes, additional, Feeney, Susan, additional, Fernandez-Garcia, Maria D, additional, Flipse, Jacky, additional, Franck, Kristina T, additional, Galli, Cristina, additional, Garrigue, Isabelle, additional, Geeraedts, Felix, additional, Georgieva, Irina, additional, Giardina, Federica, additional, Guiomar, Raquel, additional, Hauzenberger, Elenor, additional, Heikens, Esther, additional, Henquell, Cécille, additional, Hober, Didier, additional, Hönemann, Amrio, additional, Howson-Wells, Hannah, additional, Hruškar, Željka, additional, Ikonen, Niina, additional, Imbert, Berthemarie, additional, Jansz, Arjan R, additional, Jeannoël, Marion, additional, Jiřincová, Helena, additional, Josset, Laurence, additional, Keeren, Kathrin, additional, Kramer-Lindhout, Naomie, additional, Krokstad, Sidsel, additional, Lazrek, Mouna, additional, Le Guillou-Guillemette, Hélène, additional, Lefeuvre, Caroline, additional, Lind, Andreas, additional, Lunar, Maja M, additional, Maier, Melanie, additional, Marque-Juillet, Stéphanie, additional, McClure, C Patrick, additional, McKenna, James, additional, Meijer, Adam, additional, Menasalvas Ruiz, Ana, additional, Mengual-Chuliá, Beatriz, additional, Midgley, Sofie, additional, Mirand, Audrey, additional, Molenkamp, Richard, additional, Montes, Milagrosa, additional, Moreno-Docón, Antonio, additional, Morley, Ursula, additional, Murk, Jean-Luc, additional, Navascués-Ortega, Ana, additional, Nijhuis, Roel, additional, Nikolaeva-Glomb, Lubomira, additional, Nordbø, Svein A, additional, Numanovic, Sanela, additional, Oggioni, Massimo, additional, Oñate Vergara, Eider, additional, Pacaud, Jordi, additional, Pacreau, Marie L, additional, Panning, Marcus, additional, Pariani, Elena, additional, Pekova, Lili, additional, Pellegrinelli, Laura, additional, Petrovec, Miroslav, additional, Pietsch, Corinna, additional, Pilorge, Léa, additional, Piñeiro, Luis, additional, Piralla, Antonio, additional, Poljak, Mario, additional, Prochazka, Birgit, additional, Rabella, Nuria, additional, Rahamat-Langendoen, Janette C, additional, Rainetova, petra, additional, Reynders, Marijke, additional, Riezebos-Brilman, Annelies, additional, Roorda, Lieuwe, additional, Savolainen-Kopra, Carita, additional, Schuffenecker, Isabelle, additional, Smeets, Leo C, additional, Stoyanova, Ayse, additional, Stefic, Karl, additional, Swanink, Caroline, additional, Tabain, Irena, additional, Tjhie, Jeroen, additional, Thouault, Luc, additional, Tumiotto, Camille, additional, Uceda Renteria, Sara, additional, Uršič, Tina, additional, Vallet, Sophie, additional, Van Ranst, Marc, additional, Van Wunnik, Peter, additional, Verweij, Jaco J, additional, Vila, Jorgina, additional, Wintermans, Bas, additional, Wollants, Elke, additional, Wolthers, Katja C, additional, Xavier López-Labrado, F, additional, Fischer, Thea Kolsen, additional, Harvala, Heli, additional, and Benschop, Kimberley S M, additional

Published
2024
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11. Circulation, viral diversity and genomic rearrangement in mpox virus in the Netherlands during the 2022 outbreak and beyond

Author

Schuele, Leonard, primary, Boter, Marjan, additional, Nieuwenhuijse, David F., additional, Götz, Hannelore, additional, Fanoy, Ewout, additional, de Vries, Henry, additional, Vieyra, Bruno, additional, Bavalia, Roisin, additional, Hoornenborg, Elske, additional, Molenkamp, Richard, additional, Jonges, Marcel, additional, van den Ouden, Anton, additional, Simões, Margarida, additional, van den Lubben, Mariken, additional, Koopmans, Marion, additional, Welkers, Matthijs R. A., additional, and Oude Munnink, Bas B., additional

Published
2024
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12. Clinical Evaluation of Roche SD Biosensor Rapid Antigen Test for SARS-CoV-2 in Municipal Health Service Testing Site, the Netherlands

Author

Igloi, Zsofia, Velzing, Jans, van Beek, Janko, van de Vijver, David, Aron, Georgina, Ensing, Roel, Benschop, Kimberley, Han, Wanda, Boelsums, Timo, Koopmans, Marion, Geurtsvankessel, Corine, and Molenkamp, Richard

Subjects
Biosensors -- Evaluation, Immune system -- Testing, Molecular diagnostic techniques -- Evaluation, Health
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged >1 year ago (1) but still keeps a strong grip not only on daily life but also on diagnostic capacities. Reverse transcription [...]

Published
2021
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13. Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

Author

López-Labrador, F. Xavier, Brown, Julianne R., Fischer, Nicole, Harvala, Heli, Van Boheemen, Sander, Cinek, Ondrej, Sayiner, Arzu, Madsen, Tina Vasehus, Auvinen, Eeva, Kufner, Verena, Huber, Michael, Rodriguez, Christophe, Jonges, Marcel, Hönemann, Mario, Susi, Petri, Sousa, Hugo, Klapper, Paul E., Pérez-Cataluňa, Alba, Hernandez, Marta, Molenkamp, Richard, der Hoek, Lia van, Schuurman, Rob, Couto, Natacha, Leuzinger, Karoline, Simmonds, Peter, Beer, Martin, Höper, Dirk, Kamminga, Sergio, Feltkamp, Mariet C.W., Rodríguez-Díaz, Jesús, Keyaerts, Els, Nielsen, Xiaohui Chen, Puchhammer-Stöckl, Elisabeth, Kroes, Aloys C.M., Buesa, Javier, Breuer, Judy, Claas, Eric C.J., and de Vries, Jutte J.C.

Published
2021
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14. COVID-19 in health-care workers in three hospitals in the south of the Netherlands: a cross-sectional study

Author

Sikkema, Reina S, Pas, Suzan D, Nieuwenhuijse, David F, O'Toole, Áine, Verweij, Jaco, van der Linden, Anne, Chestakova, Irina, Schapendonk, Claudia, Pronk, Mark, Lexmond, Pascal, Bestebroer, Theo, Overmars, Ronald J, van Nieuwkoop, Stefan, van den Bijllaardt, Wouter, Bentvelsen, Robbert G, van Rijen, Miranda M L, Buiting, Anton G M, van Oudheusden, Anne J G, Diederen, Bram M, Bergmans, Anneke M C, van der Eijk, Annemiek, Molenkamp, Richard, Rambaut, Andrew, Timen, Aura, Kluytmans, Jan A J W, Oude Munnink, Bas B, Kluytmans van den Bergh, Marjolein F Q, and Koopmans, Marion P G

Published
2020
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18. Duration and key determinants of infectious virus shedding in hospitalized patients with coronavirus disease-2019 (COVID-19)

Author

van Kampen, Jeroen J. A., van de Vijver, David A. M. C., Fraaij, Pieter L. A., Haagmans, Bart L., Lamers, Mart M., Okba, Nisreen, van den Akker, Johannes P. C., Endeman, Henrik, Gommers, Diederik A. M. P. J., Cornelissen, Jan J., Hoek, Rogier A. S., van der Eerden, Menno M., Hesselink, Dennis A., Metselaar, Herold J., Verbon, Annelies, de Steenwinkel, Jurriaan E. M., Aron, Georgina I., van Gorp, Eric C. M., van Boheemen, Sander, Voermans, Jolanda C., Boucher, Charles A. B., Molenkamp, Richard, Koopmans, Marion P. G., Geurtsvankessel, Corine, and van der Eijk, Annemiek A.

Published
2021
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21. DC-SIGN Polymorphisms Associate with Risk of Hepatitis C Virus Infection Among Men who Have Sex with Men but not Among Injecting Drug Users

Author

MSM Observational Study of Acute Infection with Hepatitis C (MOSAIC) Study Group and Amsterdam Cohort Studies (ACS), Steba, Gaby S., Koekkoek, Sylvie M., Vanhommerig, Joost W., Brinkman, Kees, Kwa, David, Van Der Meer, Jan T. M., Prins, Maria, Berkhout, Ben, Tanck, Michael, Paxton, William A., Molenkamp, Richard, and Schinkel, Janke

Published
2018

22. HIV-1 exposure and immune activation enhance sexual transmission of Hepatitis C virus by primary Langerhans cells

Author

Nijmeijer, Bernadien M., Sarrami-Forooshani, Ramin, Steba, Gaby S., Schreurs, Renee RCE., Koekkoek, Sylvie M., Molenkamp, Richard, Schinkel, Janke, Reiss, Peter, van Heukelom, Matthijs L. Siegenbeek, van der Valk, Marc, Ribeiro, Carla MS., and Geijtenbeek, Teunis BH.

Subjects
Langerhans cells -- Health aspects, HIV infections -- Development and progression, Disease transmission -- Development and progression -- Research -- Analysis -- Health aspects -- Risk factors, MSM (Men who have sex with men) -- Health aspects, Hepatitis C -- Risk factors, Immune response -- Health aspects, Health
Abstract

Introduction: The significant rise in incidence of Hepatitis C virus (HCV) infection among men-who-have-sex-with-men (MSM) living with HIV-1 suggests that HCV under specific circumstances is transmitted via sexual contact. During sexual transmission HCV has to cross the epithelial barrier to either directly enter the blood stream or indirectly via mucosal immune cells. However, the mechanisms of sexual transmission of HCV remain unclear. We investigated the role of Langerhans cells (LCs) in HCV susceptibility during sexual contact as LCs are among the first cells in mucosal tissues to encounter invading viruses. Methods: We investigated the phenotype of primary LCs in anal biopsies from MSM living with HIV-1. To investigate the role of primary LCs in HCV infection and transmission, we have used both isolated primary skin LCs and the ex vivo tissue transmission model. Results: Our data identified an important role for mucosal LCs in facilitating HCV transmission after HIV-1 exposure or immune activation. LCs were detected within mucosal anal tissues obtained from HIV-1 positive MSM biopsies. In order to perform functional studies, we used primary LCs from skin, which have a similar phenotype as mucosal LCs. Immature LCs were neither infected nor transmitted HCV to hepatocytes. Notably, exposure to HIV-1 significantly increased HCV transmission by LCs in the ex vivo transmission model. HIV-1 replication was crucial for the increased HCV transmission as HIV-1 inhibitors significantly reduced HIV-1-induced HCV transmission. Moreover, tissue immune activation of LCs also increased HCV transmission to target cells. Conclusions: Thus, our data strongly indicate that HIV-1 or immune activation in MSM leads to capture of HCV by mucosal LCs, which might facilitate transmission to other cells or allow entry of HCV into the blood. This novel transmission mechanism by LCs also implicates that the activation state of LCs is an important cellular determinant for HCV susceptibility after sexual contact. Keywords: HIV-1; Langerhans Cells; Hepatitis C virus; Immune activation; Viral transmission; Men-who-have-Sex-with-Men, 1 | INTRODUCTION Globally, 115 million people are infected with Hepatitis C virus (HCV) whereas 37 million people are infected with HIV-1, and approximately two million infected with both HIV-1 [...]

Published
2019
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23. Mpox outbreak among men who have sex with men in Amsterdam and Rotterdam, the Netherlands: no evidence for undetected transmission prior to May 2022, a retrospective study

Author

de Vries, Henry J, primary, Götz, Hannelore M, additional, Bruisten, Sylvia, additional, van der Eijk, Annemiek A, additional, Prins, Maria, additional, Oude Munnink, Bas B, additional, Welkers, Matthijs RA, additional, Jonges, Marcel, additional, Molenkamp, Richard, additional, Westerhuis, Brenda M, additional, Schuele, Leonard, additional, Stam, Arjen, additional, Boter, Marjan, additional, Hoornenborg, Elske, additional, Mulders, Daphne, additional, van den Lubben, Mariken, additional, and Koopmans, Marion, additional

Published
2023
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24. Delayed Laboratory Response to COVID-19 Caused by Molecular Diagnostic Contamination

Author

Mogling, Ramona, Meyer, Adam, Berginc, Natasa, Bruisten, Sylvia, Charrel, Remi, Coutard, Bruno, Eckerle, Isabella, Enouf, Vincent, Hungnes, Olav, Korukluoglu, Gulay, Kossyvakis, Thanos, Mentis, Andreas, Molenkamp, Richard, Muradrasoli, Shaman, Papa, Anna, Pigny, Fiona, Thirion, Laurence, van der Werf, Sylvie, and Reusken, Chantal

Subjects
Severe acute respiratory syndrome -- Diagnosis, Coronaviruses -- Health aspects, COVID-19 -- Diagnosis, Health
Abstract

Timely and reliable laboratory diagnosis is crucial for clinical care and to inform public health responses in the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic (1). The laboratory [...]

Published
2020
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26. Safety, tolerability, and antiviral effect of RG-101 in patients with chronic hepatitis C: a phase 1B, double-blind, randomised controlled trial

Author

van der Ree, Meike H, de Vree, J Marleen, Stelma, Femke, Willemse, Sophie, van der Valk, Marc, Rietdijk, Svend, Molenkamp, Richard, Schinkel, Janke, van Nuenen, Ad C, Beuers, Ulrich, Hadi, Salah, Harbers, Marten, van der Veer, Eva, Liu, Kai, Grundy, John, Patick, Amy K, Pavlicek, Adam, Blem, Jacqueline, Huang, Michael, Grint, Paul, Neben, Steven, Gibson, Neil W, Kootstra, Neeltje A, and Reesink, Hendrik W

Published
2017
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27. Diagnostic accuracy of SARS-CoV-2 rapid antigen self-tests in asymptomatic individuals in the omicron period: a cross-sectional study

Author

Venekamp, Roderick P., primary, Schuit, Ewoud, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K., additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D., additional, Zwart, Vivian F., additional, Lodder, Esther B., additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn J.H., additional, Vroom, Irene H., additional, Smeets, Leonard C., additional, Nagel-Imming, Carla R.S., additional, Han, Wanda G.H., additional, van den Hof, Susan, additional, Kluytmans, Jan A.J.W., additional, van de Wijgert, Janneke H.H.M., additional, and Moons, Karel G.M., additional

Published
2023
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28. Standardization of SARS-CoV-2 Nucleic Acid Amplification Techniques by Calibration and Quantification to the First WHO International Standard for SARS-CoV-2 RNA

Author

Voermans, Jolanda J. C., primary, Mulders, Daphne G. J. C., additional, Beerkens, Rob J. J., additional, Duijn, Marlize van, additional, Zwaan, Liesbeth van der, additional, Rahamat-Langendoen, Janette, additional, Eijk, Annemiek van der, additional, Koopmans, Marion P. G., additional, and Molenkamp, Richard, additional

Published
2023
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32. Human Monkeypox Virus outbreak among Men who have Sex with Men in Amsterdam and Rotterdam, the Netherlands: no evidence for undetected transmission prior to May 2022 in a retrospective study

Author

de Vries, Henry J., primary, Götz, Hannelore M., additional, Bruisten, Sylvia, additional, van der Eijk, Annemiek A., additional, Prins, Maria, additional, Oude Munnink, Bas B., additional, Welkers, Matthijs R.A., additional, Jonges, Marcel, additional, Molenkamp, Richard, additional, Westerhuis, Brenda M., additional, Schuele, Leonard, additional, Stam, Arjen, additional, Boter, Marjan, additional, Hoornenborg, Elske, additional, Mulders, Daphne, additional, van den Lubben, Mariken, additional, and Koopmans, Marion, additional

Published
2022
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34. Assay optimization for molecular detection of Zika virus/ Optimisation des tests pour la detection moleculaire du virus Zika/ Optimizacion de la prueba para la deteccion molecular del virus de Zika

Author

Corman, Victor M., Rasche, Andrea, Baronti, Cecile, Aldabbagh, Souhaib, Cadar, Daniel, Reusken, Chantal BEM, Pas, Suzan D., Goorhuis, Abraham, Schinkel, Janke, Molenkamp, Richard, Kiimmerer, Beate M., Bleicker, Tobias, Brunink, Sebastian, Eschbach-Bludau, Monika, Eis-Hubinger, Anna M., Koopmans, Marion P., Schmidt-Chanasit, Jonas, Grobusch, Martin P., de Lamballerie, Xavier, Drosten, Christian, and Drexler, Jan Felix

Subjects
Biological assay -- Innovations, Genotype -- Identification and classification, Molecular diagnostic techniques -- Innovations, Health
Abstract

Objective To examine the diagnostic performance of real-time reverse transcription (RT)-polymerase chain reaction (PCR) assays for Zika virus detection. Methods We compared seven published real-time RT-PCR assays and two new assays that we have developed. To determine the analytical sensitivity of each assay, we constructed a synthetic universal control ribonucleic acid (uncRNA) containing all of the assays' target regions on one RNA strand and spiked human blood or urine with known quantities of African or Asian Zika virus strains. Viral loads in 33 samples from Zika virus-infected patients were determined by using one of the new assays. Findings Oligonucleotides of the published real-time RT-PCR assays, showed up to 10 potential mismatches with the Asian lineage causing the current outbreak, compared with 0 to 4 mismatches for the new assays. The 95% lower detection limit of the seven most sensitive assays ranged from 2.1 to 12.1 uncRNA copies/reaction. Two assays had lower sensitivities of 17.0 and 1373.3 uncRNA copies/reaction and showed a similar sensitivity when using spiked samples. The mean viral loads in samples from Zika virus-infected patients were 5 x 104 RNA copies/mL of blood and 2 x [10.sup.4] RNA coples/mL of urine. Conclusion We provide reagents and updated protocols for Zika virus detection suitable for the current outbreak strains. Some published assays might be unsuitable for Zika virus detection, due to the limited sensitivity and potential incompatibility with some strains. Viral concentrations in the clinical samples were close to the technical detection limit, suggesting that the use of insensitive assays will cause false-negative results. Objectif Etudier la performance diagnostique des tests bases sur l'amplification en chaine par polymerase (PCR) en temps reel apres transcription inverse (RT) pour detecter le virus Zika. Methodes Nous avons compare sept tests publies utilisant la RT-PCR en temps reel et deux nouveaux tests developpes par nos soins. Afin de determiner la sensibilite analytique de chaque test, nous avons concu un acide ribonucleique synthetique de controle universel (ARNcun) contenant toutes les regions ciblees par les tests sur une helice ARN et enrichi de l'urine ou du sang humain de quantites connues de souches africaines ou asiatiques du virus Zika. Les charges virales dans 33 echantillons provenant de patients infectes par le virus Zika ont ete determinees a l'aide de l'un des nouveaux tests. Resultats Les oligonucleotides des tests publies utilisant la RT-PCR en temps reel ont presente jusqu'a TOmauvais apparlements potentiels avec la lignee asiatique provoquant l'epidemie actuelle, alors qu'on a constate de 0 a 4 mauvais appariements dans le cas des nouveaux tests. La limite de detection inferieure a 95% des sept tests les plus sensibles variait de 2,1 a 12,1 copies dARNcun/reaction. Deux tests presentaient des sensibilites plus basses de 17,0 et 1373,3 copies d'ARNcun/reaction et montraient une sensibilite similaire lorsque des echantillons enrichis etaient utilises. Les charges virales moyennes dans les echantillons provenant de patients infectes par le virus Zika etaient de 5 x 104 copies d'ARN/mL de sang et de 2 x 104 copies dARN/mL d'urine. Conclusion Nous proposons pour detecter le virus Zika des reactifs et des protocoles actualises, adaptes aux souches responsables de la flambee actuelle.Tous les tests publies ne permettent pas de detecter le virus Zika en raison d'une sensibilite limitee et d'une incompatibilite potentielle avec certaines souches. Les concentrations virales dans les echantillons cliniques etaient proches de la limite de detection technique, ce qui laisse penser que l'utilisation de tests insensibles donnera des resultats faussement negatifs. Objetivo Examinar el rendimiento del diagnostico de las pruebas de reaccion en cadena de la polimerasa de transcriptasa inversa (RT-PCR) en tiempo real para la deteccion del virus de Zika. Metodos Se compararon siete pruebas de RT-PCR en tiempo real publicadas y dos pruebas nuevas que se han desarrollado. Con el fin de determinar la sensibilidad analitica de cada prueba, se construyo un acido ribonucleico sintetico de control universal (uncRNA) que contenia todas las regiones objetivo de las pruebas en una hebra de RNA y se anadio a sangre u orina humana con cantidades conocidas de cepas de virus de Zika de Asia o Africa. Se determinaron las cargas viricas en 33 muestras procedentes de pacientes infectados por el virus de Zika a traves de una de las pruebas nuevas. Resultados Los oligonucleotidos de las pruebas de RT-PCR en tiempo real publicadas mostraron hasta 10 posibles discordancias con el linaje de Asia causante de los brotes actuales, en comparacion con 0 de 4 discordancias en el caso de las pruebas nuevas. El limite de deteccion inferior del 95% de las siete pruebas mas sensibles abarco de 2,1 a 12,1 copias/reaccion de uncRNA. Dos pruebas mostraron sensibilidades inferiores de 17,0 y 1373,3 copias/reaccion de uncRNA y presentaron una sensibilidad similar al usar muestras Infectadas. Las cargas viricas medias en las muestras procedentes de pacientes infectados por el virus de Zika fueron de 5 x 104 copias de RNA/mL de sangre y de 2 x [10.sup.4] coplas de RNA/ml de orina. Conclusion Se proporcionan reactivos y protocolos actualizados para la deteccion adecuada del virus de Zika en el caso de las cepas de brotes actuales. Algunas pruebas publicadas pueden no ser adecuadas para la deteccion del virus de Zika, debido a la limitada sensibilidad y a la posible incompatibilidad con algunas cepas. Las concentraciones viricas en las muestras clinicas se acercaron al limite de deteccion tecnico, lo que sugeria que el uso de pruebas Intensivas causaria resultados falsos negativos., Introduction The Zika virus is a mosquito-borne flavivirus with an approximately 11 kilobase ribonucleic acid (RNA) genome. (1) The virus usually causes a mild infection in adults, symptoms include fever, [...]

Published
2016
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36. Antigenic cartography of SARS-CoV-2 reveals that Omicron BA.1 and BA.2 are antigenically distinct

Author

Mykytyn, Anna Z., primary, Rissmann, Melanie, additional, Kok, Adinda, additional, Rosu, Miruna E., additional, Schipper, Debby, additional, Breugem, Tim I., additional, van den Doel, Petra B., additional, Chandler, Felicity, additional, Bestebroer, Theo, additional, de Wit, Maurice, additional, van Royen, Martin E., additional, Molenkamp, Richard, additional, Oude Munnink, Bas B., additional, de Vries, Rory D., additional, GeurtsvanKessel, Corine, additional, Smith, Derek J., additional, Koopmans, Marion P. G., additional, Rockx, Barry, additional, Lamers, Mart M., additional, Fouchier, Ron A. M., additional, and Haagmans, Bart L., additional

Published
2022
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37. Diagnostic accuracy of covid-19 rapid antigen tests with unsupervised self-sampling in people with symptoms in the omicron period: cross sectional study

Author

Schuit, Ewoud, primary, Venekamp, Roderick P, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K, additional, van den Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Zwart, Vivian F, additional, Lodder, Esther B, additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn J H, additional, Vroom, Irene H, additional, Smeets, Leonard C, additional, Nagel-Imming, Carla R S, additional, Han, Wanda G H, additional, van den Hof, Susan, additional, Kluytmans, Jan A J W, additional, van de Wijgert, Janneke H H M, additional, and Moons, Karel G M, additional

Published
2022
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38. Persistent Transmission of HCV among Men Who Have Sex with Men despite Widespread Screening and Treatment with Direct-Acting Antivirals

Author

Popping, Stephanie, primary, Cuypers, Lize, additional, Claassen, Mark A. A., additional, van den Berk, Guido E., additional, De Weggheleire, Anja, additional, Arends, Joop E., additional, Boerekamps, Anne, additional, Molenkamp, Richard, additional, Koopmans, Marion P. G., additional, Verbon, Annelies, additional, Boucher, Charles A. B., additional, Rijnders, Bart, additional, and van de Vijver, David A. M. C., additional

Published
2022
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39. Admissions to a large tertiary care hospital and Omicron BA.1 and BA.2 SARS-CoV-2 polymerase chain reaction positivity: primary, contributing, or incidental COVID-19

Author

Voor in ’t holt, Anne F., primary, Haanappel, Cynthia P., additional, Rahamat–Langendoen, Janette, additional, Molenkamp, Richard, additional, van Nood, Els, additional, van den Toorn, Leon M., additional, Peeters, Robin P., additional, van Rossum, Annemarie M.C., additional, and Severin, Juliëtte A., additional

Published
2022
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40. Clinical Characteristics and Outcomes of Immunocompromised Patients With Coronavirus Disease 2019 Caused by the Omicron Variant: A Prospective, Observational Study

Author

Malahe, S Reshwan K, primary, Hoek, Rogier A S, additional, Dalm, Virgil A S H, additional, Broers, Annoek E C, additional, den Hoed, Caroline M, additional, Manintveld, Olivier C, additional, Baan, Carla C, additional, van Deuzen, Charlotte M, additional, Papageorgiou, Grigorios, additional, Bax, Hannelore I, additional, Van Kampen, Jeroen J, additional, Hellemons, Merel E, additional, Kho, Marcia M L, additional, de Vries, Rory D, additional, Molenkamp, Richard, additional, Reinders, Marlies E J, additional, and Rijnders, Bart J A, additional

Published
2022
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41. Diagnostic accuracy of SARS-CoV-2 rapid antigen self-tests in asymptomatic individuals in the Omicron period: cross sectional study

Author

Venekamp, Roderick P, primary, Schuit, Ewoud, additional, Hooft, Lotty, additional, Veldhuijzen, Irene K, additional, Bijllaardt, Wouter, additional, Pas, Suzan D, additional, Zwart, Vivian F, additional, Lodder, Esther B, additional, Hellwich, Marloes, additional, Koppelman, Marco, additional, Molenkamp, Richard, additional, Wijers, Constantijn, additional, Vroom, Irene H, additional, Smeets, Leonard C, additional, Nagel-Imming, Carla RS, additional, Han, Wanda GH, additional, van den Hof, Susan, additional, Kluytmans, Jan AJ, additional, van de Wijgert, Janneke HHM, additional, and Moons, Karel GM, additional

Published
2022
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43. Respiratory Viruses in Invasively Ventilated Critically Ill Patients—A Prospective Multicenter Observational Study

Author

van Someren Gréve, Frank, Juffermans, Nicole P., Bos, Lieuwe D. J., Binnekade, Jan M., Braber, Annemarije, Cremer, Olaf L., de Jonge, Evert, Molenkamp, Richard, Ong, David S. Y., Rebers, Sjoerd P. H., Spoelstra–de Man, Angelique M. E., van der Sluijs, Koenraad F., Spronk, Peter E., Verheul, Kirsten D., de Waard, Monique C., de Wilde, Rob B. P., Winters, Tineke, de Jong, Menno D., and Schultz, Marcus J.

Published
2018
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45. SARS-CoV-2 outbreaks in secondary school settings in the Netherlands during fall 2020; silent circulation

Author

Jonker, Lotte, primary, Linde, Kimberly J, additional, de Hoog, Marieke LA, additional, Sprado, Robin, additional, Huisman, Robin, additional, Molenkamp, Richard, additional, Oude Munnink, Bas B, additional, Dohmen, Wietske, additional, Heederik, Dick JJ, additional, Eggink, Dirk, additional, Welkers, Matthijs RA, additional, Vennema, Harry, additional, Koopmans, Marion PG, additional, Fraaij, Pieter LA, additional, Wouters, Inge M, additional, and Bruijning-Verhagen, Patricia CJL, additional

Published
2022
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46. Clinical characteristics and outcome of immunocompromised patients with COVID-19 caused by the Omicron variant: a prospective observational study

Author

Malahe, S. Reshwan K., primary, Hoek, Rogier A.S., additional, Dalm, Virgil A.S.H., additional, Broers, Annoek E.C., additional, den Hoed, Caroline M., additional, Manintveld, Olivier C., additional, Baan, Carla C., additional, van Deuzen, Charlotte M., additional, Papageorgiou, Grigorios, additional, Bax, Hannelore I., additional, Van Kampen, Jeroen J., additional, Hellemons, Merel E., additional, Kho, Marcia M.L., additional, de Vries, Rory D., additional, Molenkamp, Richard, additional, Reinders, Marlies E.J., additional, and Rijnders, Bart J.A., additional

Published
2022
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47. Admissions to a large tertiary care hospital and Omicron BA.1 and BA.2 SARS-CoV-2 PCR positivity: primary, contributing, or incidental COVID-19

Author

Voor in ’t holt, Anne F., primary, Haanappel, Cynthia P., additional, Rahamat – Langendoen, Janette, additional, Molenkamp, Richard, additional, van Nood, Els, additional, van den Toorn, Leon M., additional, Peeters, Robin P., additional, van Rossum, Annemarie M.C., additional, and Severin, Juliëtte A., additional

Published
2022
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49. Additional file 1 of Head-to-head comparison of the accuracy of saliva and nasal rapid antigen SARS-CoV-2 self-testing: cross-sectional study

Author

Schuit, Ewoud, Venekamp, Roderick P., Veldhuijzen, Irene K., van den Bijllaardt, Wouter, Pas, Suzan D., Stohr, Joep J. J. M., Lodder, Esther B., Hellwich, Marloes, Molenkamp, Richard, Igloi, Zsofia, Wijers, Constantijn, Vroom, Irene H., Nagel-Imming, Carla R. S., Han, Wanda G. H., Kluytmans, Jan A. J. W., van den Hof, Susan, van de Wijgert, Janneke H. H. M., and Moons, Karel G. M.

Abstract

Additional file 1: Material 1. Short questionnaire after performing both self-tests at home. Material 2. Short questionnaire provided 10 days after test site visit. Material 3. Specimen collection, SARS-CoV-2 diagnostic testing, and SARS-CoV-2 virus culture procedures. Table S1. 2x2 tables for primary and secondary analyses of the molecular reference test – saliva Ag-RDT comparison. Table S2. 2x2 tables for primary and secondary analyses of the molecular reference test – nasal Ag-RDT comparison. Table S3. Follow-up information of participants that were tested again within 10 days after the initial test. Fig. S1. Sensitivities with 95% confidence intervals of the Ag-RDT-molecular reference standard test comparisons at viral load cut-off for infectiousness. Fig. S2. Distribution of viral loads in participants with a positive molecular reference test, stratified by symptomatology at time of sampling. Fig. S3. Distribution of change scores in difficulty of taking a sample and difficulty of reading the test result when comparing difficulty ratings of the saliva Ag-RDT and the nasal Ag-RDT.

Published
2022
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50. Additional file 1 of Detection of SARS-CoV-2 infection in the general population by three prevailing rapid antigen tests: cross-sectional diagnostic accuracy study

Author

Venekamp, Roderick P., Veldhuijzen, Irene K., Moons, Karel G. M., van den Bijllaardt, Wouter, Pas, Suzan D., Lodder, Esther B., Molenkamp, Richard, Igloi, Zsofi, Wijers, Constantijn, dos Santos, Claudy Oliveira, Debast, Sylvia B., Bruins, Marjan J., Polad, Khaled, Nagel-Imming, Carla R. S., Han, Wanda G. H., van de Wijgert, Janneke H. H. M., van den Hof, Susan, and Schuit, Ewoud

Abstract

Additional file 1 : Suppl. Table S1. Baseline characteristics of the study population included in the PanBio test with less invasive sampling technique evaluation. Suppl. Table S2. Diagnostic accuracy variables of PanBio-molecular reference standard test comparison with less invasive sampling. Values are percentages (95% confidence interval) unless stated otherwise. Suppl. Table S3. Diagnostic accuracy variables of additional secondary analyses of three rapid antigen tests, with different sampling methods. Values are percentages (95% confidence interval) unless stated otherwise. Suppl. Table S4. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the BD VeritorTM System by Becton Dickinson (���BD-Veritor���) using routine sampling (OP-N). Suppl. Table S5. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the Roche/SD Biosensor by Roche Diagnostics (���SD-Biosensor���) using routine sampling (NP). Suppl. Table S6. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the Roche/SD Biosensor by Roche Diagnostics (���SD-Biosensor���) using less invasive sampling (OP-N). Suppl. Table S7. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the PanBio by Abbot (���PanBio���) using routine sampling (NP). Suppl. Table S8. Two-by-two tables used in primary and secondary analysis to determine diagnostic accuracy parameters of the PanBio by Abbot (���PanBio���) using less invasive sampling (OP-N). Suppl. Table S9. GISAID data contributors acknowledgement table. Suppl. Material 1. Short questionnaire (translated from Dutch). Suppl. Material 2. Specimen collection, SARS-CoV-2 diagnostic testing, and SARS-CoV-2 virus culture procedures.

Published
2022
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