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The study covered lipid content of RBC membranes in divers exposed to extreme hyperbaric factors at work. Findings are that prophylactic intake of biologically active additive nutrient "Kalifen" made of viburnum berries preserved physiologic characteristics of RBC and recovery of lipid components in their membranes.

Results of the study of the impact of acetone intoxication on the fatty acids pattern of the general lipids of erythrocytes’ membranes in rats are presented. The inhalation exposure of acetone was carried out in the inoculation chamber with the volume of 100 liters. The chamber was designed for the type of B.A. Kurlyandsky with self-contained system of purification and air regeneration and specified parameters of temperature (20-22С) and air humidity. The flow rate of the air and aerosolized acetone passed through the chamber accounted of 10 liters/min. Concentration of acetone in the chamber was sustained at the level of 206 ± 3,9 mg/m that corresponds to maximum permissible concentration for acetone vapor in the air of a working area. The time of exposure was 6 hours per day for 3 weeks in a monotonous mode, excluding weekend, and was based upon specific parameters of environment simulation in industry. The acetone impact was shown to be accompanied by the gain in the quantity of all kinds of saturated fatty acids and the fall of unsaturated fatty acids in general lipids of erythrocytes ’ membranes in rats and in the structure ofphospholipid fractions. In the content of phosphatydilcholine and phosphatydilethanolamine, as a basic structural phospholipids of biological membranes, there was noted the increase in palmitic and stearic acids. In the range offatty acids of the n-6 family the amount of linoleic and arachidonic acids decreased. In the array of fatty acids of the n-3 family the content of linolenic, eicosapentaenoic and docosahexaenoic acids (n-3 family) declined. Redistribution of fatty acids in the erythrocytes membrane towards to such alteration in quantity as the increasing of saturation and decreasing of the unsaturated fatty acids supposes the change of its physical and chemical properties, permeability, lability and complexity of passing erythrocyte via microcircular channels.

Results of the research of the impact of extract from the byproducts of guelder-rose, containing a complexflavonoides (65% of dry weight), in the conditions of experimental a stress (vertical fixing of rats by a dorsal cervicalfold for 22 o’clock) are presented. The impact of a stress was shown to be followed by the gain in mass of adrenal glands by 42%. In blood serum there were noted marked hypertriglyceridemia, hypercholesterolemia, increased superoxide dismutase activity, decline in the number of reduced glutathione and anti-radical activity, the gain in lisofractions of phospholipids, decrease of phosphatidylcholine, phosphatidylethanolamine, cholesterol esters. Administration of the extract from the byproducts of guelder rose crushing promoted the normalization of indices of mass of adrenal glands and lipid metabolism in blood serum, was more effective, than a reference stress protector “Extract eleuterokokka®”. The introduction of eleuterococcus was followed by the persistent both elevated level of cholesterol, free fatty acids, lisofraction of phospholipidsand low level offatty acids esters, cholesterol esters and a diphosphatidilglicerin. Byproducts of guelder-rose were shown to represent a perspective view of raw materials for the producing of stress protective preparations.

We have studied the effects of the extract from the dry pomace of honeysuckle fruits Lonicera edulis Turcz. and commercial reference preparation Legalon on weight and biochemical liver indexes in rats upon carbon tetrachloride intoxication. The extract of honeysuckle fruits favored a decrease in the total lipid content, reduced specific liver weight, and facilitated restoration of the activity of serum alanine aminotransferase and liver lysosomal enzymes. It the blood of rats, the extract increased superoxide dismutase activity, decreased glutathione level, enhanced antiradical activity, and reduced the level of malonic dialdehyde. The effect of administration of honeysuckle fruits pomace extract on restoration of the liver function was more pronounced than the effect of Legalon.

Hepatoprotective properties of ethanol extract from the roots of Maackia amurensis Ruper et Maxim have been studied on the model of toxic hepatitis induced by carbon tetrachloride damage. It is established that the extract contains daidzein, 7-O-gentobiosides of isoflavonoids genistein, formononetin, pseudobabtige-nin, and 5-O-methylgenistein, and 3-O-gentobiosides of pterocarpans (6aR, 11aR)-maakiain and (6aR, 11aR)-medicarpin. The administration of extract facilitates the restoration of antioxidant protection enzymes activity and reduced glutathione level, decreases the formation of toxic peroxidation products, produces normalizing impact on liver phospholipid pattern, and improves the erythrocyte tolerance to hemolytic agents. The action of isoflavonoids from Maackia amurensis in restoration of metabolic pathways of the liver and removal of toxic stress was more effective as compared to that of the reference hepatoprotector legalon.

Study covered female surgeons subjected to occupational stress complex (psychoemotional, chemical, work intensity, etc). Prophylactic intake of biologically active food supplement made of high cranberry appeared to relieve tension of antioxidant and antiradical defence systems, increased activation and stress, to normalize carbohydrates metabolism parameters.

Preventive injection of "Kalifen" extract of high cranberry before and during intoxication with carbon bisulphide appeared to preserve lipid component of RBC membrane in rats.