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Your search keyword '"Monforte-González, Miriam"' showing total 42 results
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42 results on '"Monforte-González, Miriam"'

34. Direct Somatic Embryogenesis in Coffea canephora.

Author

Walker, John M., Vázquez-Flota, Felipe, Quiroz-Figueroa, Francisco, Monforte-González, Miriam, Galaz-Ávalos, Rosa M., and Loyola-Vargas, Victor M.

Abstract

Somatic embryogenesis (SE) provides a useful model to study embryo development in plants. In contrast to zygotic embryogenesis, SE can easily be observed, the culture conditions can be controlled, and large quantities of embryos can be easily obtained. In Coffea spp several model systems have been reported for in vitro SE induction. SE for coffee was first reported in Coffea canephora. Several systems have been developed since then, including SE from callus cultures derived from leaf explants; a two-phase experimental protocol for SE from leaves of Coffea arabica; and from leaf explants of Arabusta or C. arabica using a medium with cytokinins. Here we report a protocol using young leaves from in vitro seedling pre-conditioned with growth regulators. This is a simplified method to obtain a faster and more efficient protocol to produce direct somatic embryos in C. canephora. [ABSTRACT FROM AUTHOR]

Published
2006
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37. Enrichment of carbon dioxide in the atmosphere increases the capsaicinoids content in Habanero peppers ( Capsicum chinense Jacq.).

Author

Garruña‐Hernández, René, Monforte‐González, Miriam, Canto‐Aguilar, Azucena, Vázquez‐Flota, Felipe, and Orellana, Roger

Abstract

Background The effects of the increase of atmospheric CO2 on agricultural productivity have been mainly analyzed through its impact on biomass yield, and little attention has been directed to quality traits, such as nutritional or organoleptic attributes. For this study, plants of hot Habanero pepper ( Capsicum chinense Jacq.) were grown in growth chambers under three different CO2 levels: 380 (normal atmospheric value), 760 and 1140 µmol mol−1, and their effects on pod yield, size, color and pungency, were monitored. Results The total number of pods per plant increased by 88.5% at the highest CO2, in comparison to plants grown at normal CO2 conditions. Pod size and yield per plant also increased when plants were grown at the highest CO2 concentration (partial pressure). Furthermore, total capsaicinoids contents in ripe peppers under a high CO2 atmosphere were 27% higher than those from plants under lower concentrations, but it was not the case for immature pods. Conclusion These data suggest that the increase of atmospheric CO2 could modify specific routes of secondary metabolism as well as others desirable traits, thus affecting the quality of Capsicum pepper products. © 2012 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2013
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39. Capsaicinoid Quantitation by In Situ Densitometry of Thin Layer Chromatography Plates.

Author

Monforte‐González, Miriam, Medina‐Lara, Fatima, Gutiérrez‐Carbajal, Guadalupe, and Vázquez‐Flota, Felipe

Subjects
*THIN layer chromatography, *GAS chromatography, *CHROMATOGRAPHIC analysis, *PHASE partition, *PEPPERS, *AMIDES
Abstract

A method for measuring the content of total capsaicinoids by in situ densitometry on thin layer chromatography (TLC) plates is described. This method is easy to perform, reproducible, and can detect up to 0.5 µg of capsaicinoids in unpurified organic extracts. Identity of the compounds was assigned by comparing the chromatographic behavior of the putative capsaicinoids with that of standards. Under the assayed conditions, capsaicin could not be separated from dihydrocapsaicin by TLC; however, the amounts detected in different tissues was always equal to the sum of the individual compounds, as quantified by gas chromatography, suggesting that the method is reliable. [ABSTRACT FROM AUTHOR]

Published
2007
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40. Effect of Macerozyme on Secondary Metabolism Plant Product Production and Phospholipase C Activity in Catharanthus roseusHairy Roots

Author

Moreno-Valenzuela, Oscar A., Monforte-González, Miriam, Armando Muñoz-Sánchez, J., Méndez-Zeel, Marcela, Loyola-Vargas, Victor M., and Teresa Hernández-Sotomayor, S.M.

Abstract

The effect of macerozyme on accumulation of indole alkaloid and coumarine and on the induction of tryptophan decarboxylase (TDC, E.C. 4.1.1.28), phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5) and phosphatydilinositol 4,5-bisphosphate phospholipase C (PLC, E.C. 3.1.4.3) activities on Catharanthus roseushairy roots was investigated. Increasing concentrations of macerozyme induced an increase in indole alkaloid and coumarine accumulation. There was a 10-fold increase in TDC and a 4-fold increase in PAL activities in hairy roots treated with 1 % of macerozyme. In a dose-response experiment PLC activity decreased 38 % with 0.5 % of macerozyme and increased 40 % with 1 % of macerozyme when compared to untreated roots. The results demonstrate that macerozyme treatment can affect the production of secondary metabolites and PLC activity in C. roseushairy roots.

Published
1999
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42. Direct somatic embryogenesis in Coffea canephora.

Author

Quiroz-Figueroa F, Monforte-González M, Galaz-Avalos RM, and Loyola-Vargas VM

Subjects
Culture Media, Germination, Plant Leaves embryology, Seeds, Coffea embryology, Embryonic Development
Abstract

Somatic embryogenesis (SE) provides a useful model to study embryo development in plants. In contrast to zygotic embryogenesis, SE can easily be observed, the culture conditions can be controlled, and large quantities of embryos can be easily obtained. In Coffea spp several model systems have been reported for in vitro SE induction. SE for coffee was first reported in Coffea canephora. Several systems have been developed since then, including SE from callus cultures derived from leaf explants; a two-phase experimental protocol for SE from leaves of Coffea arabica; and from leaf explants of Arabusta or C. arabica using a medium with cytokinins. Here we report a protocol using young leaves from in vitro seedling pre-conditioned with growth regulators. This is a simplified method to obtain a faster and more efficient protocol to produce direct somatic embryos in C. canephora.

Published
2006
Full Text
View/download PDF

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