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2 results on '"Monica T.A. Kakimori"'

1. Co-infection by tick-borne pathogens and Leishmania spp. in dogs with clinical signs suggestive of leishmaniasis from an endemic area in northeastern Brazil

Author

Anna Maria C.F. Evaristo, Paula Talita T. Santos, Felipe S. Sé, Flávia Carolina M. Collere, Bianca B.F. Silva, Ennya R.N. Cardoso, Monica T.A. Kakimori, Thállitha Samih W.J. Vieira, Felipe S. Krawczak, Jonas Moraes-Filho, Rafael F.C. Vieira, and Mauricio C. Horta

Subjects
Ehrlichia, Anaplasma, hemotropic Mycoplasma, Babesia, Leishmania, diagnosis, dogs, leishmaniasis, Veterinary medicine, SF600-1100
Abstract

ABSTRACT: The present study aimed to investigate the occurrence of Leishmania spp., hemotropic Mycoplasma spp., tick-borne pathogens (TBP), and co-infection in dogs with clinical signs suggestive of visceral leishmaniasis (VL). It also aimed to determine the factors associated with infection and to map the distribution of co-infected dogs in an endemic area in the Northeast region of Brazil. Blood samples from 168 dogs were evaluated for serological analysis to Leishmania spp., Anaplasma spp., Ehrlichia spp., Babesia spp., and molecular assays to Leishmania spp., Anaplasma platys, Ehrlichia canis, Babesia spp., and hemotropic Mycoplasma spp. In serological and molecular analysis, 29.8% and 5.9% of dogs were co-infected. In the regression analysis, seropositivity for Ehrlichia spp., Babesia spp., and Leishmania spp. was significantly associated with the presence of petechiae, young dogs, and weight loss. Serology revealed that co-exposure with Babesia spp. and Ehrlichia spp. was associated with fever and thrombocytopenia, and there was an association between seropositivity for Ehrlichia spp. and Babesia spp. in dogs seropositive for Leishmania spp. The presence of hemotropic Mycoplasma spp. DNA was associated with anorexia. Thus, dogs with clinical VL have co-infection with other pathogens, reinforcing the importance of this study for a better understanding of these co-infections in dogs from endemic areas.

Published
2024
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2. First molecular detection of Mycoplasma ovis in horses from Brazil

Author

Monica T.A. Kakimori, Luiz D. Barros, Flávia C.M. Collere, Larissa D.R. Ferrari, Aldair de Matos, Juliana I. Lucas, Vanessa S. Coradi, Anna C.B. Mongruel, Daniel M. Aguiar, Rosangela Z. Machado, Marcos R. André, Thállitha S.W.J. Vieira, and Rafael F.C. Vieira

Subjects
Sheep, Veterinary (miscellaneous), Ehrlichia, Theileriasis, Infectious Diseases, Mycoplasma, Ticks, Insect Science, Babesiosis, RNA, Ribosomal, 16S, Theileria, Animals, Parasitology, Cattle, Horse Diseases, Horses, Brazil
Abstract

This study aimed to determine the occurrence of hemoplasmas and tick-borne pathogens (TBP) (Theileria equi, Babesia caballi, and Ehrlichia sp.) in horses and ticks' salivary glands, and determine the factors associated with exposure/infection in a rural settlement in southern Brazil. Blood samples from 22 horses were screened for anti-T. equi and anti-Ehrlichia sp. antibodies by an indirect fluorescent antibody test (IFAT) assays. Samples were also tested by PCR assays for T. equi and B. caballi (18S rRNA and rap-1 genes, respectively), hemoplasmas (16S rRNA gene), and Ehrlichia sp. (dsb gene). Ticks were removed from the animals (inspection) and the environment (flannel trawling and dry ice traps), and morphologically identified. Additionally, salivary glands DNA was extracted from 28 adult ticks infesting the animals and four nymphs from the environment, and further screened for Ehrlichia sp. and hemotropic Mycoplasma sp. Anti-T. equi and anti-Ehrlichia sp. antibodies were detected in 40.91% (nine/22; 95% CI: 23.26-61.27) and 31.81% (seven/22; 95% CI: 16.36-52.68) horses, respectively. Theileria equi, B. caballi, and hemotropic Mycoplasma sp. DNA was detected in 59.09% (13/22), 4.55% (one/22), and 50% (11/22) horses, respectively. All horses tested negative in the PCR for Ehrlichia sp. All sequences showed ≥99% identity with multiple T. equi, B. caballi, and Mycoplasma ovis sequences deposited in GenBank database. Adult ticks were identified as Dermacentor nitens (44/47; 93.62%) and Rhipicephalus microplus (three/47; 6.38%). Ticks' salivary glands were negative for Ehrlichia sp., while 39.29% from adults (11/28) and 50% from nymphs (two/four) from the environment were positive for hemotropic Mycoplasma sp. This is the first report of M. ovis infection in horses from Brazil and the first detection of hemoplasma DNA in salivary glands of D. nitens and R. microplus ticks. Further studies are needed to elucidate the vector competence of ticks to transmit hemoplasmas.

Published
2022

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