1. Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites
- Author
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Delane C. Kritsky, Silvia Maria Doglia, Anna Villa, Giovanni Strona, Paolo Galli, Fabrizio Stefani, Francesca Benzoni, Galli, P, Strona, G, Villa, A, Benzoni, F, Stefani, F, Doglia, S, and Kritsky, D
- Subjects
Kuhnia scombri ,Principal Component Analysis ,Biometry ,Microscopy, Confocal ,Laser scanning ,Confocal ,Fishes ,Anatomy ,Biology ,law.invention ,Fish Diseases ,Infectious Diseases ,Imaging, Three-Dimensional ,Microscopy, Fluorescence ,Confocal microscopy ,law ,Platyhelminths ,Monogenoidea, 3D ,Image Processing, Computer-Assisted ,Animals ,Parasitology ,Dactylogyrus extensus ,Helminthiasis, Animal ,Spatial Orientations ,Biomedical engineering - Abstract
A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations. © 2006 Australian Society for Parasitology Inc. more...
- Published
- 2007