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1. Identification of Laboratory Biomarkers for Early Detection and Clinical Management of Post-Acute Syndrome Among Survivors of the 2013–2016 West Africa Ebola Outbreak in Sierra Leone

Author

Guetiya Wadoum RE, Sevalie S, Baimba Kargbo M, Clarke A, Bangura S, Kargbo M, Sesay HM, Kamara AH, Bangura J, Kamara AF, Allieu S, Rogers H, Mattei M, Colizzi V, Montesano C, and Momoh EJJ

Subjects
ebola virus disease, hematological, immunological, biochemical, biomarkers, post-acute syndrome, Diseases of the blood and blood-forming organs, RC633-647.5
Abstract

Raoul Emeric Guetiya Wadoum,1,&ast; Stephen Sevalie,2,&ast; Maurice Baimba Kargbo,3 Andrew Clarke,4,5 Sherry Bangura,6 Mariatu Kargbo,6 Hawa Mariama Sesay,7 Abdul H Kamara,6 Jamil Bangura,6 Alie F Kamara,6 Sophie Allieu,1 Hassan Rogers,1 Maurizio Mattei,8 Vittorio Colizzi,9 Carla Montesano,8 Edwin JJ Momoh1,3 1Department of Public Health, Microbiology and Immunology, Ernest Bai Koroma University of Science and Technology, Makeni, Sierra Leone; 2 34th Military Hospital, The Republic of Sierra Leone Armed Forces, Freetown, Sierra Leone; 3Department of Agriculture and Food Security; Ernest Bai Koroma University of Science and Technology, Makeni, Sierra Leone; 4Global Programs Division, Save the Children UK, London, UK; 5Department/Division of Health Research, Lancaster University, Lancaster, UK; 6Sierra Leone Association of Ebola Survivors, Freetown, Sierra Leone; 7Department of Public Health, University of Makeni, Makeni, Sierra Leone; 8Department of Biology, University of Rome Tor Vergata, Rome, Italy; 9Department of Sciences and Technology, Evangelical University of Cameroon, Bandjoun, Cameroon&ast;These authors contributed equally to this workCorrespondence: Raoul Emeric Guetiya Wadoum; Edwin JJ Momoh, Email ergwadoum@ebkustsl.edu.sl; raoulemeric@gmail.com; emomoh@ebkust.edu.slBackground: The clinical management of persistent medical conditions affecting Ebola survivors, generally described as a post-Ebola syndrome, remains a public health concern. We aimed to analyze Ebola survivors’ laboratory biomarkers as compared to their non-infected household relatives to identify biomarkers that could guide the identification of survivors at increased risk of developing severe at odds with the non-severe post-Ebola syndrome.Materials and Methods: Data were extracted from medical records of the Ebola survivors clinic, and we included only Ebola survivor’s parameters recorded during the first baseline follow-up visit 2 weeks interval after their second negative PCR result. Moreover, household non-infected family contacts of survivors visiting the clinic during the same period were recruited as community control.Results: The mean age of survivors was 32.65 (IQR: 15.5, 38.25) years, and Ebola IgG immunoglobulin was detected in all, thus confirming their status. The statistical significance (all p < 0.05) observed in monocyte percentage (MONO%), cluster of differentiation 4 percentage (CD4%), alanine aminotransferase (ALT), creatinine (CREA), and creatinine kinase (C-kinase) proved to be clinically significant as compared to the household relatives’ group. Interestingly, the linear regression analysis indicated that the duration at ETU was negatively associated with lymphocyte percentage with a 5% lymphocyte decrease per day spent at ETU. Finally, there was a significant (p < 0.05) association between hematological (Hb, PCV, MCV, MCH), biochemical (ALT, CREA, C-kinase, T-cholesterol, triglycerides) parameters and the risk of developing severe complications.Conclusion: We recommend clinicians closely monitor Hb, PCV, MCV, MCH, ALT, CREA, C-kinase, T-cholesterol, triglycerides and lymphocytes as clinically relevant laboratory biomarkers to identify survivors at higher risk of developing severe post-acute syndrome upon discharge from Ebola treatment unit including headache, abdominal pain, chest pain, ocular complication, arthralgia, hearing difficulty and erectile dysfunction which can impact health-related quality of life among Ebola survivors.Keywords: Ebola virus disease, hematological, immunological, biochemical, biomarkers, post-acute syndrome

Published
2023

2. The 2018–2020 Ebola Outbreak in the Democratic Republic of Congo: A Better Response Had Been Achieved Through Inter-State Coordination in Africa

Author

Guetiya Wadoum RE, Sevalie S, Minutolo A, Clarke A, Russo G, Colizzi V, Mattei M, and Montesano C

Subjects
united nations, africa union, who, africa cdc, ebola virus disease, covid-19, Public aspects of medicine, RA1-1270
Abstract

Raoul Emeric Guetiya Wadoum,1 Stephen Sevalie,2 Antonella Minutolo,3 Andrew Clarke,4,5 Gianluca Russo,6 Vittorio Colizzi,7 Maurizio Mattei,8 Carla Montesano8 1Department of Public Health, Microbiology and Immunology, Ernest Bai Koroma University of Science and Technology, Makeni, Sierra Leone; 2 34th Regimental Military Hospital, The Republic of Sierra Leone Armed Forces, Freetown, Sierra Leone; 3Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy; 4Global Programs Division, Save the Children United Kingdom, London, UK; 5Division of Health Research, Lancaster University, Lancaster, UK; 6Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy; 7Department of Biomedical Sciences, Faculty of Science and Technology, Evangelical University of Cameroon, Bandjoun, Cameroon; 8Department of Biology, School of Mathematics, Physics and Natural Sciences, University of Rome Tor Vergata, Rome, ItalyCorrespondence: Raoul Emeric Guetiya Wadoum Tel +232 78425924Email ergwadoum@ebkustsl.edu.sl; raoulemeric@gmail.comAbstract: The Ebola virus disease, formerly known as Ebola hemorrhagic fever, is a severe and often fatal zoonosis in humans. The 2013– 2016 West African Ebola outbreak had distinctive characteristics, and it was the largest and most complex epidemic since the virus discovery in 1976. Although the 2018– 2020 Ebola outbreak in the Democratic Republic of the Congo had many similarities, there were additional challenges due to the presence of armed rebel groups at the epicenters of the epidemic. Despite these challenges, the extraordinary commitment of the World Health Organization (WHO) regional office for Africa, in collaboration with Africa Union (AU) member states through the Africa Centres for Disease Control and Prevention (Africa CDC), and WHO’s prompt declaration of a Public Health Emergency of International Concern (PHEIC) shepherded an effective coordinated response to contain the epidemic. Learning from previous Ebola virus epidemics and the current Coronavirus disease 2019 (COVID-19) pandemic, the AU member states should strengthen inter-state coordination towards the development and implementation of a preparedness and readiness plan which will enable the continent to build and sustain resilient capacities to prevent, detect, and respond to future outbreaks following the International Health Regulations (IHR).Keywords: United Nations, Africa Union, WHO, Africa CDC, Ebola virus disease, COVID-19

Published
2021

5. Exogenous miRNAs from Moringa oleifera Lam. recover a dysregulated lipid metabolism

Author

Roglia, V, Potesta, M, Minchella, A, Bruno, Sp, Bernardini, R, Lettieri-Barbato, D, Iacovelli, F, Gismondi, A, Aquilano, K, Canini, A, Muleo, R, Colizzi, V, Mattei, M, Minutolo, A, and Montesano, C

Subjects
Inflammation, Settore BIO/01, lipid metabolism, plant miRNAs, cross-kingdom interactions, post transcriptional modification, Settore MED/04, Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria, Biochemistry, Genetics and Molecular Biology (miscellaneous), Molecular Biology, Biochemistry
Abstract

A balanced diet is critical for human health, and edible plants play an important role in providing essential micronutrients as well as specific microRNAs (miRNAs) that can regulate human gene expression. Here we present the effects of Moringa oleifera (MO) miRNAs (mol-miRs) on lipid metabolism. Through in silico studies we identified the potential genes involved in lipid metabolism targeted by mol-miRs. To this end, we tested the efficacy of an aqueous extract of MO seeds (MOES), as suggested in traditional African ethnomedicine, or its purified miRNAs. The biological properties of MO preparations were investigated using a human derived hepatoma cell line (HepG2) as a model. MOES treatment decreased intracellular lipid accumulation and induced apoptosis in HepG2. In the same cell line, transfection with mol-miRs showed similar effects to MOES. Moreover, the effect of the mol-miR pool was investigated in a pre-obese mouse model, in which treatment with mol-miRs was able to prevent dysregulation of lipid metabolism.

Published
2022

12. Massa 2022 - Book of Abstracts

Author

Di Francesco, G., Croce, M., Vincenti, F., Montesano, C., Sergi, M., and Curini, R.

Subjects
Suspect Screening, Novel Psychoactive Substances, Mass Spectrometry, Suspect Screening, Novel Psychoactive Substances, Mass Spectrometry
Published
2022

17. Modular deployable structures

Author

Nieto, J., primary, Fayos, J., additional, Pipó, A., additional, Montesano, C., additional, Ozores, E., additional, and Santiago-Prowald, J., additional

Published
2022
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20. RRI in Africa

Author

Montesano, C and Colizzi, V

Subjects
Settore MED/04
Published
2019

26. Current use and acceptability of novel diagnostic tests for active tuberculosis: A worldwide survey|Uso atual e aceitabilidade de novos testes diagnósticos para tuberculose ativa: Um inquérito mundial

Author

Amicosante, M, D'Ambrosio, L, Munoz, M, Mello, Fcq, Tebruegge, M, Chegou, Nn, Seghrouchni, F, Centis, R, Goletti, D, Bothamley, G, Migliori, Gb, Al-Hattami, Aam, Kebede, A, Rendon, A, Garcia-Basteiro, Al, Abdulmumuni, As, Nota, A, Pace-Asciak, A, Loxton, A, Bhowmik, A, Catanzaro, A, Worku, A, Kabeer, Bsa, Savic, B, Nselebete, Bb, Montesano, C, Montagnani, C, Mammina, C, Nkenfou, C, Kvasnovsky, C, Braz, C, Blazquez-Gamero, D, Cirillo, Dm, Huluka, Dk, Handayani, D, Papaventsis, D, Palmero, D, Catanzaro, D, Chanda, D, Gurbanova, E, Sobh, E, Gebremariam, Fm, Pradel, Fk, Brindicci, G, Sotgiu, G, De Iaco, G, Kahenya, G, Nwankwo, G, Hoffmann, H, Mutunzi, H, Kenechukwu, I, Pavic, I, Mukeba, J, Wambugu, J, Abdulsemed, Ka, Ukwaja, Kn, Fattorini, L, Guglielmetti, L, Zammarchi, L, Anibarro, L, Zijenah, Ls, Lipman, M, Ivanovska, M, Nikolova, M, Joya, Mc, Bonnet, M, Chisale, M, Jachym, Mf, Elmi, M, Gadanya, Ma, Getahun, M, Veziris, N, Hanson-Nortey, Nn, Nwankwo, Ono, Del Monte, P, Fourati, R, Laniado-Laborin, R, Lodha, R, Wong, R, Bhebhe, R, de la Tour, R, Ferreira, Rt, Boy, S, Eyanu, S, Singh, S, Tafaj, S, Joosten, Sa, Callens, S, Moyo, S, Tao, Tm, Gebremariam, Th, Meshak, Tp, Perez-Porcuna, T, Vasankari, T, Rolla, Vc, Singh, V, Ratageri, Vh, Milanov, V, Rosati, Y, and Fuentes-Alcala, Z

Subjects
Adult, Male, Attitude of Health Personnel, Female, Global Health, Humans, Interviews as Topic, Middle Aged, Reagent Kits, Diagnostic, Sensitivity and Specificity, Tuberculosis, Young Adult, Mycobacterium tuberculosis, Settore MED/04, Reagent Kits, Diagnostic
Published
2017

28. Nutraceutical plant derived microrna elements for treatment of leukemia

Author

Colizzi, V, Marco, C, Galgani, A, Flavia DEL GALLO DI ROCCAGIOVINE, Maurice, K, Minutolo, A, Montesano, C, Rosario, M, Stefano, P, Potesta', M, and Matic, I

Subjects
Moringa oleifera, Olea europea, leukemia, plant microRNAs (miRNAs), Settore MED/04, mammalian microRNAs (miRNAs), medicinal plants
Published
2016

29. Awareness of kidney diseases in general population and in high school students. Italian report for World Kidney Days 2010-2011

Author

Apperti, V, Auricchio, Mr, Barbato, A, Bedani, Pl, Bellinghieri, G, Costantino, G, Boggi, R, Bonomini, M, Calabria, L, Calabria, M, Grimaldi, M, Capuano, M, Terribile, M, Caputo, C, Castellino, S, Centrone, E, Ciccarelli, M, Cicchetti, T, Costantino, E, D'Amaro, E, Dagostino, F, Napolitano, F, Bonifati, C, Dal Canton, A, Esposito, P, Delgado, G, D'Apice, L, Di Luca, M, Farfaglia, P, Cantù, P, Farina, M, Fasianos, E, Feriozzi, S, Galeotti, P, Fiorini, F, Frattolillo, P, Garibotto, Giacomo, Giannattasio, M, Detomaso, F, Latino, A, Li Vecchi, M, Maffucci, G, Anelli, Am, Mangano, S, Meneghel, G, Morrone, L, Mura, C, Novizio, D, Paglia, S, Cardone, F, Parsi, R, Pizzini, M, Polito, P, Prati, E, Brognoli, M, Rubino, F, Turchetta, L, Parravano, M, Sambati, M, Sicignano, M, Tarchini, R, Teatini, U, Gallieni, M, Colussi, G, Limido, A, Pozzi, C, Spotti, D, Brancaccio, D, Traversari, L, Venditti, G, Aucella, F, Garganico, R, Giovanni Rotondo, S, Cenerelli, S, Bozzi, M, Petrarulo, F, Casu, Md, Cavatorta, F, Montesano, C, Ferrara, Dd, Mazzola, Ma, De Simone, W, Del Rosso, G, Sozzo, Ee, Mangione, D, Emiliani, G, Fasianos, R, Ganadu, M, Garibotto, G, Lusenti, T, Manno, C, Orbello, G, Rondanini, V, Russo, D, Battaglia, Y, Sarli, P, Sozzo, E, Napoli, M, Valentini, Wd, Viganò, L., Y., Battaglia 1, L., Russo 1, R., Spadola 1, Russo, Domenico, Battaglia, Y, Russo, L., and Spadola, R.

Subjects
Male, Nephrology, Health Knowledge, Attitudes, Practice, Pediatrics, Kidney Disease, Global Health, Surveys and Questionnaires, Prevalence, Global health, Surveys and Questionnaire, Young adult, Reagent Strips, Practice, Kidney, education.field_of_study, Health Knowledge, Urinalysi, Awareness, Middle Aged, Proteinuria, Treatment Outcome, medicine.anatomical_structure, Italy, Hypertension, Kidney Diseases, Female, Reagent Strip, Student, Comprehension, Human, Adult, medicine.medical_specialty, Adolescent, Population, Health knowledge, Health literacy, Health Promotion, Urinalysis, Young Adult, Terminology as Topic, Internal medicine, medicine, Humans, Students, education, Aged, business.industry, Blood Pressure Determination, Health Literacy, Adolescent Behavior, Awarene, Health promotion, Attitudes, Family medicine, business
Published
2012

33. Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Author

Battista, N, Sergi, M, Montesano, C, Napoletano, S, Compagnone, D, and Maccarrone, M

Subjects
Brain Chemistry, Cannabinoid Receptor Agonists, Meconium, Polyunsaturated Alkamides, Arachidonic Acids, Δ9-tetrahydrocannabinol,anandamide,liquid chromatography,mass spectrometry, Mass Spectrometry, Milk, Animals, Humans, Dronabinol, Chromatography, Liquid, Endocannabinoids, Hair
Abstract

Over the last two decades, the role played by phytocannabinoids and endocannabinoids in medicine has gained increasing interest in the scientific community. Upon identification of the plant compound Δ(9)-tetrahydrocannabinol (THC) and of the endogenous substance anandamide (AEA), different methodological approaches and innovative techniques have been developed, in order to evaluate the content of these molecules in various human matrices. In this review, we discuss the analytical methods that are currently used for the identification of phytocannabinoids and endocannabinoids, and we summarize the benefits and limitations of these procedures. Moreover, we provide an overview of the main biological matrices that have been analyzed to date for qualitative detection and quantitative determination of these compounds.

Published
2014

34. A High Density of Mutations within HLA Class I and II HBsAg/RT Epitopes Characterizes HBV Variants Emerging during Immune-Suppression Driven HBV Reactivation

Author

Salpini, R., primary, Grifoni, A., additional, Lo Presti, A., additional, Minutolo, A., additional, Battisti, A., additional, Colagrossi, L., additional, Amicosante, M., additional, Colizzi, V., additional, Marrone, A., additional, Coppola, N., additional, Sarmati, L., additional, Andreoni, M., additional, Angelico, M., additional, Ciccozzi, M., additional, Perno, C.F., additional, Montesano, C., additional, and Svicher, V., additional

Published
2016
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35. Selection of microbial epitopes for immune recognition. North-South transfer in Biotechnology of Tuberculosis and AIDS. University of Rome 'Tor Vergata'. UNESCO Interdisciplinary Chair in Biotechnology. Vittorio Colizzi, Luc Mantagnier, 2004

Author

AMICOSANTE M, MONTESANO C, GOLETTI G, GIOIA C, VINCENTI D, CARRARA S, PUCILLO LP, DIELI, Francesco, CACCAMO, Nadia Rosalia, SALERNO, Alfredo, COLIZZI V., MONTAGNIER L., AMICOSANTE M, DIELI F, MONTESANO C, GOLETTI G, GIOIA C, VINCENTI D, CARRARA S, CACCAMO N, SALERNO A, and PUCILLO LP

Published
2004

36. Development of a post-exposure paediatric anti HIV-AIDS vaccine based on the combined synthetic/recombinant HIV peptides and BCG for boosting innate and acquired immunity. North-South transfer in Biotechnology of Tuberculosis and AIDS. University of Rome 'Tor Vergata'. UNESCO Interdisciplinary Chair in Biotechnology. Vittorio Colizzi, Luc Montagnier, 2004

Author

AMICOSANTE M, ANDREONI M, ANSELMI A, CASTELLI G, CIARAMELLA A, COLIZZI V, D'ARRIGO R, ERCOLI L, FRAZIANO M, GARG SK, MARCHIONE P, MATTEI M, PALMIERI G, PERNO F, SALTINI C, SANTUCCI M, SUMERSKA T, ROSSI P, VENDETTI S, CAPPELLI G, CAVONE A, GRASSI M, MARIANI F, MIGNONE M, PERRETTA G, CASETTI R, HOREJSH D, MARTINI F, MONTESANO C, GIOIA C, POCCIA F, PUCILLO L, SACCHI A, DI SANO C, CAIRO C, GALLO R, REDFIELD R, PAUZA D, CHENAL H, MONTAGNIER L, OLIVIER R, TONI T, MAULE L, SIMPORE J., DI GIORGIO, Giuseppa, MARTINO, Andrea, BONANNO, Cesira, DIELI, Francesco, SALERNO, Alfredo, COLIZZI V., MONTAGNIER L., AMICOSANTE M, ANDREONI M, ANSELMI A, CASTELLI G, CIARAMELLA A, COLIZZI V, D'ARRIGO R, DI GIORGIO G, ERCOLI L, FRAZIANO M, GARG SK, MARCHIONE P, MATTEI M, PALMIERI G, PERNO F, SALTINI C, SANTUCCI M, SUMERSKA T, ROSSI P, VENDETTI S, CAPPELLI G, CAVONE A, GRASSI M, MARIANI F, MIGNONE M, PERRETTA G, CASETTI R, HOREJSH D, MARTINO A, MARTINI F, MONTESANO C, GIOIA C, POCCIA F, PUCILLO L, SACCHI A, BONANNO CT, DIELI F, DI SANO C, SALERNO A, CAIRO C, GALLO R, REDFIELD R, PAUZA D, CHENAL H, MONTAGNIER L, OLIVIER R, TONI T, MAULE L, and SIMPORE J

Published
2004

37. Risk factors for kidney diseases and awareness of blood pressure and proteinuria in general population and in high school students: Italian report for World Kidney Days 2012-2013

Author

Agate, *Square Project: V. M., Anelli, A. M., Apicella, L., Avino, D., Battaglia, Y., Bedani, P. L., Bellinghieri, Guido, Bernardi, A. M., Bonifati, C., Borzumati, M., Botti, P. L., Brigante, M., Brighina, F., Calzavara, P., Caputo, C., Cardone, F., Cavatorta, F., Confessore, N., Cossu, M., Costantino, E., Costantino, Giuseppe, D’Apice, L., D’Arcangelo, R., Dagostino, F., Dal Canton, A., Delgado, G., Di Pietro, R., Esposito, C., Esposito, P., Fasianos, E., Fiorini, F., Galeotti, P., Garibotto, G., Gemelli, A., Gregorini, M. C., Iuliano, P., La Peccerella, L., Liuzzi, M., Merola, M., Montesano, C., Morrone, L. F., Napoli, M., Napolitano, F., Paglia, S., Parravano, M., Pasquali, S., Rosa, A., Sambati, M. L., Schiavone, P., Sozzo, E., Storari, A., Tarchini, R., Tirino, G., Bellinghieri, L. T. u. r. c. h. e. t. t. a. School Project: G., Beltrame, G., Bozzi, M., Casolino, E., Centrone, E., Ciccarelli, M., Cicchetti, T., Colturi, C., Costantino, G., Garibotto, Indraccolo, F., Lombardi, M., Minoretti, C., Parsi, R., Petrarulo, F., Prati, E., Quarello, F., Rondanini, V., Russo, D., Tira, P., Turina, S., and Valentini, W. D.

Subjects
Adult, Male, Pathology, medicine.medical_specialty, Health Knowledge, Attitudes, Practice, Adolescent, Population, Health Promotion, Risk Factors, Medicine, Humans, Renal Insufficiency, Chronic, Renal Insufficiency, Chronic, education, Students, education.field_of_study, Practice, business.industry, Health Knowledge, Middle Aged, Proteinuria, Italy, Nephrology, Cardiovascular Diseases, Attitudes, Female, Kidney Diseases, business, Humanities
Abstract

*Square Project: V.M. Agate (Palmanova, UD), A.M. Anelli (Pistoia), L. Apicella (Salerno), D. Avino (Vairano Scalo, CE), Y. Battaglia (Ferrara), P.L. Bedani (Ferrara), G. Bellinghieri (Messina), A.M. Bernardi (Rovigo), C. Bonifati (Corato, BA), M. Borzumati (Verbania), P.L. Botti (Mantova), M. Brigante (Campobasso), F. Brighina (San Nicola La Strada, CE), P. Calzavara (Conegliano, TV), C. Caputo (Albenga, SV), F. Cardone (Lavello, PZ), F. Cavatorta (Imperia), N. Confessore (Scafati, SA), M. Cossu (Sassari), E. Costantino (Gavardo, BS), G. Costantino (Messina), L. D’Apice (Caserta), R. D’Arcangelo (Arzano, NA), F. Dagostino (Corato, BA), A. Dal Canton (Pavia), G. Delgado (Teano, CE), R. Di Pietro (Napoli), C. Esposito (Pavia), P. Esposito (Pavia), E. Fasianos (Altamura, BA), F. Fiorini (Rovigo), P. Galeotti (Viterbo), G. Garibotto (Genova), A. Gemelli (Rovigo), M.C. Gregorini (Reggio Emilia), P. Iuliano (Benevento), L. La Peccerella (Benevento), M. Liuzzi (Benevento), M. Merola (Sorrento, NA), C. Montesano (Imperia), L.F. Morrone (Benevento), M. Napoli (Galatina, LE), F. Napolitano (Corato, BA), S. Paglia (Lavello, PZ), M. Parravano (Sora, FR), S. Pasquali (Reggio Emilia), A. Rosa (Benevento), M.L. Sambati (Taranto), P. Schiavone (Brindisi), E. Sozzo (Galatina, LE), A. Storari (Ferrara), R. Tarchini (Mantova), G. Tirino (Montesarchio, BN), L. Turchetta (Sora, FR). School Project: G. Bellinghieri (Messina), G. Beltrame (Torino), M. Bozzi (Bari), E. Casolino (Rionero in Vulture, PZ), E. Centrone (Ruvo di Puglia, BA), M. Ciccarelli (Reggio Calabria), T. Cicchetti (Rossano, CS), C. Colturi (Sondrio), E. Costantino (Gavardo, BS), G. Costantino (Messina), F. Dagostino (Corato, BA), E. Fasianos (Altamura, BA), P. Galeotti (Viterbo), Garibotto (Genova), F. Indraccolo (S. Fermo della Battaglia, CO), M. Lombardi (Borgo S. Lorenzo, FI), C. Minoretti (S. Fermo della Battaglia, CO), F. Napolitano (Corato, BA), R. Parsi (Alcamo, TP), F. Petrarulo (Bari), E. Prati (Desenzano del Garda, BS), F. Quarello (Torino), V. Rondanini (Palmi, RC), D. Russo (Barletta), P. Tira (Manerbio, BS), S. Turina (Manerbio, BS), W.D. Valentini (Rieti) WORLD KIDNEY DAY

Published
2013

41. Neonatal protection and preterm birth reduction following maternal group B streptococcus vaccination in a mouse model.

Author

Bernardini, R., Aufieri, R., Detcheva, A., Recchia, S., Cicconi, R., Amicosante, M., Montesano, C., Rossi, P., Tchidjou, H. K., Petrunov, B., Orefici, G., and Mattei, M.

Subjects
PREMATURE labor, STREPTOCOCCUS agalactiae, PREGNANCY complications, PREGNANCY in mammals, IMMUNIZATION of infants, VACCINATION, BACTERIAL vaccines, GESTATIONAL age, ENZYME-linked immunosorbent assay, PREVENTION of communicable diseases, PREVENTION of pregnancy complications, STREPTOCOCCAL disease prevention, ANIMAL experimentation, ANIMAL populations, BIOLOGICAL models, COMMUNICABLE diseases, IMMUNIZATION, PREMATURE infants, MATERNALLY acquired immunity, MICE, STREPTOCOCCAL diseases, STREPTOCOCCUS, PREVENTION
Abstract

Purpose: Evaluate effects of maternal immunization in a mouse model of Group B Streptococcus (GBS) vaginal colonization using clinical isolates.Materials and Methods: Female pregnant mice were immunized with heat-killed GBS 21 days before pregnancy and were inoculated intravaginally with GBS cultures (5 × 107 CFU twice a day for three days) from the 16th day of pregnancy. Gestation period and mice survival were monitored. Maternal anti-GBS IgG levels have been determined by ELISA analysis in vaccinated, unvaccinated mothers and newborns.Results: Maternal immunization before pregnancy provided protection to newborns for three of the four GBS strains used. Evaluation of the immunogenicity showed that this vaccination induced higher levels of IgG in vaccinated compared to unvaccinated dams and the presence of antibodies in the offspring at embryonic and postnatal age, and a Th1 response and high levels of IgG2a subclass antibody and IFN-γ were detected. A significant reduction of preterm births was observed in vaccinated mothers (p< 0.05).Conclusions: Our finding suggest that vaccinated mothers could protect their progeny from GBS infection and preterm birth through passive immunization. The proposed mouse model may represent a noninvasive and effective tool to investigate pathogenetic mechanisms of GBS ascending infection and for vaccine protection studies. [ABSTRACT FROM AUTHOR]

Published
2017
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48. Computer-based design of an HLA-haplotype and HIV-clade independent cytotoxic T-lymphocyte assay for monitoring HIV-specific immunity

Author

Amicosante M, Gioia C, Montesano C, Casetti R, Topino S, D'Offizi G, Cappelli G, Giuseppe Ippolito, Colizzi V, Poccia F, and Lp, Pucillo

Subjects
Male, Biological Assay, Middle Aged, Peptides, Female, T-Lymphocytes, Cytotoxic, Interferon-gamma, Gene Products, gag, Humans, HLA Antigens, Molecular Sequence Data, Adult, Amino Acid Sequence, HIV, Settore MED/04 - Patologia Generale, gag, Cytotoxic, T-Lymphocytes, virus diseases, Gene Products, Research Article
Abstract

BACKGROUND: Human immunodeficiency virus (HIV)- specific CD8-positive cytotoxic T-lymphocytes (CTL) play a key role in controlling HIV infection. Monitoring CTL response could be clinically relevant during structured therapy interruption (STI), HIV exposure, and vaccine trials. However, HLA patients' restriction and HIV variability limited the development of a CTL assay with broad specificity. MATERIALS AND METHODS: We designed an HLA-class I/HIV-1 clade independent assay for assessing HIV- specific CTL by using a computer-assisted selection ofthe CTL epitopes. Twenty-eight 15-mers were selected by peptide-binding motifs analysis using different databases (HIV-Immunology Database, SYFPEITHI, BIMAS). Altogether they putatively bind to more than 90% of HLA haplotypes in different populations, with an overall HIV-1 variability below 9%. The peptide pool was used as an antigen in an intracellular cytokine staining (ICS) assay for quantifying HIV-specific CTL response. RESULTS: The test can be performed using both fresh and cryopreserved peripheral blood mononuclear cells (PBMC), whereas GAG protein as antigen works only on fresh PBMC. A significantly higher CTL response with respect to HIV-negative controls was detected in all HIV-1 infected subjects of two groups of patients with different ethnicities (Caucasians and Africans) and coming from areas with different HIV-1 clade prevalences (clade B and A/G, respectively). In Caucasian patients, after month of STI, the number of HIV-1 specific CTL (2,896 +/- 2,780 IFN-gamma specific CD8 cells/ml) was significantly higher than that found at enrolment (2,125 +/- 4,426 IFN-gamma specific CD8 cells/ml, p< 0.05). CONCLUSIONS: These data indicate that this CTL assay is broadly specific and could represent a useful clinical tool for HIV immunodiagnostic independent of HLA-haplotype and HIV-clade variabilities.

Published
2002

49. Innate T-cell immunity in HIV infection: the role of Vgamma9Vdelta2 T lymphocytes

Author

Poccia, F, Gougeon, M, Agrati, C, Montesano, C, Martini, F, Pauza, C, Fisch, P, Wallace, M, and Malkovsky, M

Subjects
Settore MED/04 - Patologia Generale, gamma-delta, B-Lymphocytes, Acquired Immunodeficiency Syndrome, T-Lymphocytes, Receptors, Antigen, T-Cell, gamma-delta, Antiviral Agents, HIV Infections, Animals, Killer Cells, Natural, Humans, Polymorphism, Genetic, Antiretroviral Therapy, Highly Active, Cell Differentiation, Cytokines, Models, Biological, Ligands, Antiretroviral Therapy, T-Cell, Biological, Genetic, Models, Antigen, Receptors, Natural, Killer Cells, Highly Active, Polymorphism
Published
2002

50. Antiviral activity and anergy of gammadeltaT lymphocytes in cord blood and immuno-compromised host

Author

Montesano, C, Gioia, C, Martini, F, Agrati, C, Cairo, C, Pucillo, L, Colizzi, V, and Poccia, F

Subjects
Adult, Settore MED/04 - Patologia Generale, gamma-delta, C-Type, Tumor Necrosis Factor-alpha, T-Lymphocytes, Infant, HIV Infections, T-Cell, Fetal Blood, Newborn, CD, Interferon-gamma, T-Lymphocyte, HLA Antigens, Antigen, Differentiation, Lectins, Receptors, Immune Tolerance, Humans, Interleukin-2, Receptors, Antigen, T-Cell, gamma-delta, Antigens, Differentiation, T-Lymphocyte, Antigens, CD, Receptors, Interleukin-2, Infant, Newborn, Lectins, C-Type, Antigens
Published
2001

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