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El objetivo de este estudio fue desarrollar un método sencillo y eficaz mediante la extracción de un disolvente convencional y precipitación anti-disolvente para el aislamiento de licopeno a partir de Lycopersicon esculentum. Un total de 100 gramos de polvo liofilizado de L. esculentum se pesaron en un tubo de vidrio con un filtro de vidrio en el fondo. El material se extrajo durante una hora con un litro de los disolventes hexano, acetato de etilo y etanol. Los rendimientos de extracción fueron 3.58, 4.39 y 1.25 mg/g, respectivamente, lo que indica que el acetato de etilo fue el más eficiente de los disolventes de extracción estudiados. Todos los carotenoides, excepto licopeno, fueron separados del extracto crudo utilizando un método de salificación anti-disolvente (metanol). El licopeno precipitado y aislado fue de 3.50 mg/g, que es una tasa de recuperación del 77.43%. El método de aislamiento de licopeno desarrollado en este estudio fue más efi caz que los métodos ya disponibles para el aislamiento a gran escala de la preparación de licopeno.

The potential hair growth-promoting activity of rice bran supercritical CO2 extract (RB-SCE) and major components of RB-SCE, linoleic acid, policosanol, γ-oryzanol, and γ-tocotrienol, were evaluated with the histological morphology and mRNA expression levels of cell growth factors using real-time reverse transcriptase-polymerase chain reaction (PCR) in C57BL/6 mice. RB-SCE showed hair growth-promoting potential to a similar extent as 3% minoxidil, showing that the hair follicles were induced to be in the anagen stage. The numbers of the hair follicles were significantly increased. In addition, mRNA expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and keratinocyte growth factor (KGF) were also significantly increased and that of transforming growth factor-β (TGF-β) decreased in RB-SCE-treated groups. Among the major components of RB-SCE, linoleic acid and γ-oryzanol induced the formation of hair follicles according to examination of histological morphology and mRNA expression levels of cell growth factors. In conclusion, our results demonstrate that RB-SCE, particularly linoleic acid and γ-oryzanol, promotes hair growth and suggests RB-SCE can be applied as hair loss treatment.

To evaluate the potential hair growth-promoting activity and the expression of cell growth factors of Lycopersicon esculentum extracts, each 3% (w/w) of ethyl acetate extract (EAE), and supercritical CO2 extract (SCE) of L. esculentum and isolated lycopene Tween 80 solution (LTS) and test hair tonic (THT) containing LTS were applied on the dorsal skin of C57BL/6 mice, once a day for 4 weeks. At week 4, LTS and THT exhibited hair growth-promoting potential similar to that of 3% minoxidil as a positive control (PC). Further, in the LTS group, a significant increase of mRNA expression of vascular endothelial growth factor (VEGF), keratinocyte growth factor, and insulin-like growth factor-1 (IGF-1) was observed than PC, as well as the negative control (NC). In the THT group, increases in IGF-1 and decrease in VEGF and transforming growth factor-β expression were significant over the NC. In a histological examination in the THT group, the induction of anagen stage of hair follicles was faster than that of NC. In the Draize skin irritation study for THT, no observable edema or erythema was observed on all four sectors in the back skin after exposure for 24 or 72 h for any rabbit. Therefore, this study provides reasonable evidence that L. esculentum extracts promote hair growth and suggests that applications could be found in hair loss treatments without skin irritation at moderate doses.