324 results on '"Moreira LF"'
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2. Network European society of cardiology task force conflict of Interest Policies and Disclosure Requirements Among European Society of Cardiology National Cardiovascular Journals
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Alfonso F, Timmis A, Pinto FJ, Ambrosio G, Ector H, Kulakowski P, Vardas P, Loizos Antoniades L, Ahmad M, Apetrei E, Arai K, Artigou JY, Aschermann M, Böhm M, Bolognese L, Cohen A, Edes I, Elias J, Galeano J, Guarda E, Haouala H, Heras M, Höglund C, Huber K, Hulin I, Ivanusa M, Krittayaphong R, Kuo CT, Lau CP, Lyusov VA, Marinskis G, Márquez MF, Masic I, Pinho Moreira LF, Mrochek A, Oganov RG, Raev D, Rogava M, Rødevand O, Sansoy V, Shimokawa H, Shumakov VA, Tajer CD, van der Wall EE, Stefanadis C, Videbæk J, Lüscher TF, BUGIARDINI, RAFFAELE, Alfonso F, Timmis A, Pinto FJ, Ambrosio G, Ector H, Kulakowski P, Vardas P, Loizos Antoniades L, Ahmad M, Apetrei E, Arai K, Artigou JY, Aschermann M, Böhm M, Bolognese L, Bugiardini R, Cohen A, Edes I, Elias J, Galeano J, Guarda E, Haouala H, Heras M, Höglund C, Huber K, Hulin I, Ivanusa M, Krittayaphong R, Kuo CT, Lau CP, Lyusov VA, Marinskis G, Márquez MF, Masic I, Pinho-Moreira LF, Mrochek A, Oganov RG, Raev D, Rogava M, Rødevand O, Sansoy V, Shimokawa H, Shumakov VA, Tajer CD, van der Wall EE, Stefanadis C, Videbæk J, and Lüscher TF
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conflict of interest - Abstract
no abstract available
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- 2012
3. Conflitti di interesse e requisiti di trasparenza nell'ambito delle Riviste Nazionali di Cardiologia dei paesi membri della Societa Europea di Cardiologia
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Alfonso F, Timmis A, Pinto FJ, Ambrosio G, Ector H, Kulakowski P, Vardas P, Loizos Antoniades L, Ahmad M, Apetrei E, Arai K, Artigou JY, Aschermann M, Böhm M, Bolognese L, Cohen A, Edes I, Elias J, Galeano J, Guarda E, Haouala H, Heras M, Höglund C, Huber K, Hulin I, Ivanusa M, Krittayaphong R, Kuo CT, Lau CP, Lyusov VA, Marinskis G, Márquez MF, Masic I, Pinho Moreira LF, Mrochek A, Oganov RG, Raev D, Rogava M, Rødevand O, Sansoy V, Shimokawa H, Shumakov VA, Tajer CD, van der Wall EE, Stefanadis C, Videbæk J, Lüscher TF, BUGIARDINI, RAFFAELE, Alfonso F, Timmis A, Pinto FJ, Ambrosio G, Ector H, Kulakowski P, Vardas P, Loizos Antoniades L, Ahmad M, Apetrei E, Arai K, Artigou JY, Aschermann M, Böhm M, Bolognese L, Bugiardini R, Cohen A, Edes I, Elias J, Galeano J, Guarda E, Haouala H, Heras M, Höglund C, Huber K, Hulin I, Ivanusa M, Krittayaphong R, Kuo CT, Lau CP, Lyusov VA, Marinskis G, Márquez MF, Masic I, Pinho-Moreira LF, Mrochek A, Oganov RG, Raev D, Rogava M, Rødevand O, Sansoy V, Shimokawa H, Shumakov VA, Tajer CD, van der Wall EE, Stefanadis C, Videbæk J, and Lüscher TF
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conflict of interest - Abstract
Disclosure of potential conflicts of interest (COI) is used by biomedical journals to guarantee credibility and transparency of the scientific process. COI disclosure, however, is not systematically nor consistently dealt with by journals. Recent joint editorial efforts paved the way towards the implementation of uniform vehicles for COI disclosure. This paper provides a comprehensive editorial perspective on classical COI-related issues. New insights into current COI policies and practices among European Society of Cardiology national cardiovascular journals, as derived from a cross-sectional survey using a standardised questionnaire, are discussed.
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- 2012
4. Respiratory mechanics and morphometric changes during pneumoperitoneum in normal rats
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Moreira, LF, primary, Gobbi, CF, additional, Feijoo, M, additional, Rocco, PR, additional, and Zin, WA, additional
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- 1997
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5. Respiratory system, lung, and chest wall mechanics after longitudinal laparotomy in rats
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Moreira, LF, primary, Aires, ST, additional, Gobbi, CF, additional, Saldiva, PH, additional, and Zin, WA, additional
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- 1995
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6. Left cardiac sympathetic denervation for treatment of symptomatic systolic heart failure patients: a pilot study.
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Conceiçao-Souza GE, Pêgo-Fernandes PM, Cruz Fd, Guimaraes GV, Bacal F, Vieira ML, Grupi CJ, Giorgi MC, Consolim-Colombo FM, Negrao CE, Rondon MU, Moreira LF, and Bocchi EA
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- 2012
7. Prognostic value of cardiopulmonary exercise testing in children with heart failure secondary to idiopathic dilated cardiomyopathy in a non-beta-blocker therapy setting.
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Guimaraes GV, d'Avila VM, Camargo PR, Moreira LF, Luces JR, and Bocchi EA
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- 2008
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8. Ischemic preconditioning in myocardial revascularization with intermittent aortic cross-clamping.
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Pêgo-Fernandes PM, Jatene FB, Kwasnicka K, Hueb AC, Moreira LF, Gentil AD, Stolf NAG, Oliveira SA, Pêgo-Fernandes, P M, Jatene, F B, Kwasnicka, K, Hueb, A C, Moreira, L F, Gentil, A F, Stolf, N A, and Oliveira, S A
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- 2000
9. CORRELATION BETWEEN SYMPTOMATOLOGY AND RETROGRADORY CONDUCTION IN SICK SINUS SYNDROME (SSS) AND CARDIAC PACING
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Martinelli, M., Roberto Costa, Moreira, Lf, Sosa, E., Pileggi, F., and Bellotti, G.
10. ARRHYTHMIAS ASSOCIATED WITH ATRIOVENTRICULAR STIMULATION IN CHRONIC CHAGAS-DISEASE
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Martinelli, Fm, Roberto Costa, Moreira, Lf, Neto, Cm, Lee, Jh, Scannavaca, M., Sosa, Ea, Bellotti, G., and Pileggi, F.
11. Adherence to cervical and breast cancer programs is crucial to improving screening performance.
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Mauad EC, Nicolau SM, Moreira LF, Haikel RL Jr., Longatto-Filho A, and Baracat EC
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INTRODUCTION: Cervical and breast cancer are the most common malignancies among women worldwide. Effective screening can facilitate early detection and dramatically reduce mortality rates. The interface between those screening patients and patients most needing screening is complex, and women in remote areas of rural counties face additional barriers that limit the effectiveness of cancer prevention programs. This study compared various methods to improve compliance with mass screening for breast and cervical cancer among women in a remote, rural region of Brazil. METHODS: In 2003, a mobile unit was used to perform 10,156 mammograms and Papanicolaou smear tests for women living in the Barretos County region of Sao Paulo state, Brazil (consisting of 19 neighbouring cities). To reach the women, the following community outreach strategies were used: distribution of flyers and pamphlets; media broadcasts (via radio and car loudspeakers); and community healthcare agents (CHCAs) making home visits. RESULTS: The most useful intervention appeared to be the home visits by healthcare agents or CHCAs. These agents of the Family Health Programme of the Brazilian Ministry of Health reached an average of 45.6% of those screened, with radio advertisements reaching a further 11.9%. The great majority of the screened women were illiterate or had elementary level schooling (80.9%) and were of 'poor' or 'very poor' socioeconomic class (67.2%). CONCLUSIONS: Use of a mobile screening unit is a useful strategy in developing countries where local health systems have inadequate facilities for cancer screening in underserved populations. A multimodal approach to community outreach strategies, especially using CHCAs and radio advertisements, can improve the uptake of mass screening in low-income, low-educational background female populations. [ABSTRACT FROM AUTHOR]
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- 2009
12. Pravila o sukobu interesa i zahtjevi za prijavljivanjem sukoba interesa u nacionalnim kardiovaskularnim časopisima Europskog kardiološkog društva
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Fernando Alfonso, Adam Timmis, Fausto J. Pinto, Giuseppe Ambrosio, Hugo Ector, Piotr Kulakowski, Panos Vardas, null Editors' Network members, Loizos Antoniades, Mansoor Ahmad, Eduard Apetrei, Kaduo Arai, Jean-Yves Artigou, Michael Aschermann, Michael Böhm, Leonardo Bolognese, Raffaele Bugiardini, Ariel Cohen, Istvan Edes, Joseph Elias, Javier Galeano, Eduardo Guarda, Habib Haouala, Magda Heras, Christer Höglund, Kurt Huber, Ivan Hulin, Mario Ivanusa, Rungroj Krittayaphong, Chi-Tai Kuo, Chu-Pak Lau, Victor A. Lyusov, Germanas Marinskis, Manlio F. Márquez, Izet Masic, Luiz Felipe Pinho Moreira, Alexander Mrochek, Rafael G. Oganov, Dimitar Raev, Mamanti Rogava, Olaf Rødevand, Vedat Sansoy, Hiroaki Shimokawa, Valentin A. Shumakov, Carlos Daniel Tajer, Ernst E. van der Wall, Christodoulos Stefanadis, Jørgen Videbæk, Thomas F. Lüscher, Alfonso F, Timmis A, Pinto FJ, Ambrosio G, Ector H, Kulakowski P, Vardas P, Loizos Antoniades L, Ahmad M, Apetrei E, Arai K, Artigou JY, Aschermann M, Böhm M, Bolognese L, Bugiardini R, Cohen A, Edes I, Elias J, Galeano J, Guarda E, Haouala H, Heras M, Höglund C, Huber K, Hulin I, Ivanusa M, Krittayaphong R, Kuo CT, Lau CP, Lyusov VA, Marinskis G, Márquez MF, Masic I, Pinho-Moreira LF, Mrochek A, Oganov RG, Raev D, Rogava M, Rødevand O, Sansoy V, Shimokawa H, Shumakov VA, Tajer CD, van der Wall EE, Stefanadis C, Videbæk J, Lüscher TF, Antoniades L, Moreira LF, Christodoulos Stefanadis EE, Lüscher TF., Alfonso, Fernando, Timmis, Adam, Pinto, Fausto J., Ambrosio, Giuseppe, Ector, Hugo, Kulakowski, Piotr, Vardas, Pano, Antoniades, Loizo, Ahmad, Mansoor, Apetrei, Eduard, Arai, Kaduo, Artigou, Jean-Yve, Aschermann, Michael, Böhm, Michael, Bolognese, Leonardo, Bugiardini, Raffaele, Cohen, Ariel, Edes, Istvan, Elias, Joseph, Galeano, Javier, Guarda, Eduardo, Haouala, Habib, Heras, Magda, Höglund, Christer, Huber, Kurt, Hulin, Ivan, Ivanusa, Mario, Krittayaphong, Rungroj, Kuo, Chi-Tai, Lau, Chu-Pak, Lyusov, Victor A., Marinskis, Germana, Márquez, Manlio, Masic, Izet, Pinho Moreira, Luiz Felipe, Mrochek, Alexander, Oganov, Rafael G., Raev, Dimitar, Rogava, Mamanti, Rødevand, Olaf, Sansoy, Vedat, Shimokawa, Hiroaki, Shumakov, Valentin A., Tajer, Carlos Daniel, Van Der Wall, Ernst E., Stefanadis, Christodoulo, Videbæk, Jørgen, Lüscher, Thomas F., Repositório da Universidade de Lisboa, Editors' Network European Society of Cardiology Task Force, Sinica AC, Christer Höglund C, Fernando Alfonso, Adam Timmi, Fausto Pinto, Giuseppe Ambrosio, Hugo Ector, Piotr Kulakowski, Panos Antoniade, Mansoor Ahmad, Eduard Apetrei, Kaduo Arai, JeanYves Artigou, Michael Aschermann, Michael Bohm, Leonardo Bolognese, Raffaele Bugiardini, Ariel Cohen, Istvan Ede, Joseph Elia, Javier Galeano, Eduardo Guarda, Habib Haouala, Magda Hera, Christer Hoglund, Kurt Huber, Ivan Hulin, Mario Ivanusa, Rungroj Krittayaphong, ChiTai Kuo, ChuPak Lau, Victor Lyusov, Germanas Marinski, Manlio Mrquez, Izet Masic, Luiz Moreira, Alexander Mrochek, Rafael Oganov, Dimitar Raev, Mamanti Rogava, Olaf Rdevand, Vedat Sansoy, Hiroaki Shimokawa, Valentin Shumakov, Carlos Tajer, Ernst Wall, Christodoulos Stefanadi, Jrgen Videbk, and Thomas Luscher
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Journal ,lcsh:Diseases of the circulatory (Cardiovascular) system ,MEDICAL JOURNALS, CLINICAL-TRIALS, PHARMACEUTICAL-INDUSTRY, COMPETING INTERESTS, BIOMEDICAL-RESEARCH, EDITORS NETWORK, DRUG-INDUSTRY, PUBLICATION, ASSOCIATION, SPONSORSHIP ,конфликт интересов ,conflicts of interest, disclosure, editorial ethics, journals ,Periodicals ,Surveys and Questionnaires ,Credibility ,Conflict of interest ,Publishing/ethics ,Cardiology and Cardiovascular Medicine ,Pharmacology (medical) ,Societies, Medical ,раскрытие ,General Environmental Science ,Pharmaceutical industry ,Disclosures ,Editorial ethics ,Journals ,Medicine (all) ,Data Collection ,Editorial ethic ,General Medicine ,Europe ,conflict of interest ,disclosure ,editorial ethics ,journals ,Disclosure ,Authorship ,Cardiology ,Drug Industry ,Periodicals as Topic ,Research Support as Topic ,Conflict of Interest ,Editorial Policies ,medicine.medical_specialty ,журналы ,редакционная этика ,MEDLINE ,Library science ,RM1-950 ,Truth Disclosure ,Special Article ,conflicts of interest ,Cross-Sectional Studies ,Humans ,Political science ,Medical ,Internal medicine ,medicine ,Diseases of the circulatory (Cardiovascular) system ,Conflict of interest, Disclosure, Editorial ethics, Journals ,Closure (psychology) ,Drug industry ,Competing interests ,business.industry ,lcsh:RM1-950 ,Conflict of interest, disclosures, editorial ethics, journals ,Transparency (behavior) ,lcsh:Therapeutics. Pharmacology ,lcsh:RC666-701 ,RC666-701 ,sukob interesa ,prijavljivanje sukoba interesa ,urednička etika ,časopisi ,General Earth and Planetary Sciences ,Therapeutics. Pharmacology ,cardiology, conflict of interest, Europe, interpersonal communication, medical society, publication, publishing ,Societies ,business - Abstract
Biomedicinski časopisi prijavljivanje sukoba interesa (COI ・prema engl. conflict of interest) koriste kako bi jamčili vjerodostojnost i transparentnost znanstvenih postupaka. Međutim,časopisi se s prijavljivanjem COI ne bave niti sistematski niti dosljedno. Nedavna zajednička nastojanja urednika su utrla put prema implementaciji jedinstvenih načina prijave COI. Ovaj dokument prža sveobuhvatni urednički stav o klasičnim problemima vezanim za COI. Raspravlja se o novim uvidima u trenutne COI smjernice i prakse nacionalnih kardiovaskularnih časopisa Europskog kardiološkog društva koji su izvedeni iz presječnog anketnog istraživanja primjenom standardiziranog upitnika., Disclosure of potential conflicts of interest (COI) is used by biomedical journals to guarantee credibility and transparency of the scientific process. COI disclosure, however, is not systematically nor consistently dealt with by journals. Recent joint editorial efforts paved the way towards the implementation of uniform vehicles for COI disclosure. This paper provides a comprehensive editorial perspective on classical COI-related issues. New insights into current COI policies and practices among European Society of Cardiology national cardiovascular journals, as derived from a cross-sectional survey using a standardised questionnaire, are discussed.
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- 2012
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13. Femoral and acetabular revision using impacted nondemineralized freeze-dried bone allografts.
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Galia CR, Macedo CA, Rosito R, Camargo LM, Marinho DR, and Moreira LF
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- 2009
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14. Conflict of interest policies and disclosure requirements among European society of cardiology national cardiovascular journals | Políticas de conflictos de intereses y requisitos para su declaración en las revistas cardiovasculares nacionales de la sociedad Europea de cardiología
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Alfonso, F., Timmis, A., Pinto, F. J., Ambrosio, G., Ector, H., Kulakowski, P., Vardas, P., Antoniades, L., Ahmad, M., Apetrei, E., Arai, K., Artigou, J. -Y, Aschermann, M., Böhm, M., Bolognese, L., Bugiardini, R., Cohen, A., Edes, I., Elias, J., Galeano, J., Guarda, E., Haouala, H., Heras, M., Höglund, C., Huber, K., Hulin, I., Ivanusa, M., Krittayaphong, R., Kuo, C. -T, Lau, C. -P, Lyusov, V. A., Marinskis, G., Márquez, M. F., Masic, I., Pinho-Moreira, L. F., Mrochek, A., Oganov, R. G., Raev, D., Rogava, M., Rødevand, O., Vedat Sansoy, Shimokawa, H., Shumakov, V. A., Tajer, C. D., Wall, E. E., Stefanadis, C., Videbæk, J., Lüscher, T. F., Alfonso F, Timmis A, Pinto FJ, Ambrosio G, Ector H, Kulakowski P, Vardas P, Antoniades L, Ahmad M, Apetrei E, Arai K, Artigou JY, Aschermann M, Böhm M, Bolognese L, Bugiardini R, Cohen A, Edes I, Elias J, Galeano J, Guarda E, Haouala H, Heras M, Christer Höglund C, Huber K, Hulin I, Ivanusa M, Krittayaphong R, Kuo CT, Lau CP, Lyusov VA, Marinskis G, Márquez MF, Masic I, Pinho-Moreira LF, Mrochek A, Oganov RG, Raev D, Rogava M, Rødevand O, Sansoy V, Shimokawa H, Shumakov VA, Tajer CD, van der Wall EE, Stefanadis C, Videbæk J, and Lüscher TF
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Conflict of interest, Disclosure, Editorial ethics, Journals - Abstract
Disclosure of potential conflicts of interest is used by biomedical journals to guarantee credibility and transparency of the scientific process. Conflict of interest disclosure, however, is not systematically nor consistently dealt with by journals. Recent joint editorial efforts paved the way towards the implementation of uniform vehicles for conflicts of interest disclosure. This paper provides a comprehensive editorial perspective on classical conflict of interest-related issues. New insights into current conflicts of interest policies and practices among European Society of Cardiology national cardiovascular journals, as derived from a cross-sectional survey using a standardized questionnaire, are discussed.
15. Effects of Intravenous Lidocaine on Quality of Recovery After Laparoscopic Bariatric Surgery: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.
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Barbosa EC, Ortegal GHPC, Aguirre JM, Costa PRR, Ferreira LN, Moreira LF, Silva GC, Ferro Filho PPM, and Ferreira DM
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- Humans, Infusions, Intravenous, Obesity, Morbid surgery, Pain, Postoperative drug therapy, Postoperative Nausea and Vomiting prevention & control, Recovery of Function drug effects, Treatment Outcome, Anesthetics, Local administration & dosage, Anesthetics, Local therapeutic use, Bariatric Surgery, Laparoscopy, Length of Stay statistics & numerical data, Lidocaine administration & dosage, Lidocaine therapeutic use, Randomized Controlled Trials as Topic
- Abstract
This systematic review and meta-analysis aimed to assess the effects of pre and intraoperative lidocaine infusion on short-term recovery quality after laparoscopic bariatric surgeries. In the search across MEDLINE, Embase, and Cochrane databases, we considered randomized controlled trials comparing intravenous lidocaine vs placebo (saline) for patients with obesity undergoing laparoscopic bariatric surgery. Seven studies (640 patients) were included. The lidocaine group had a significantly higher recovery quality score, a lower morphine consumption, and a notably reduced rate of nausea and vomiting compared with the placebo group. Additionally, Lidocaine infusion was associated with a shorter hospital stay, while no significant difference was observed in the time to bowel function recovery between both groups. In conclusion, lidocaine infusion before and during laparoscopic bariatric surgery contributes to an enhanced quality of recovery., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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16. Insights on molecular modeling and supramolecular arrangement of bilastine polymorphs.
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Santin LG, Moreira LF, Oliveira NVC, Paiva VLA, Ribeiro MR, Oliveira SS, and Napolitano HB
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Context: The advancement in the development of second-generation drugs in the field of antihistamines represents a significant milestone in the management of allergic diseases, targeting the effects of histamine. The efficacy of bilastine in treating allergic disorders has sparked interest in investigating its polymorphism, a crucial property that impacts quality, safety, and effectiveness as per regulatory guidelines. This study examines the polymorphism of bilastine, focusing on two crystalline forms labeled as Form I and Form II. Utilizing advanced analytical techniques, the research explores the structural characteristics and molecular interactions within these forms. Geometric parameters, such as bond lengths, bond angles, and torsion angles, are examined to comprehend molecular conformations and crystal packing arrangements. Hydrogen bonding, covalent bonds, and van der Waals forces contribute to the unique supramolecular arrangements in these forms. This study provides a significant contribution to understanding bilastine's polymorphism, offering critical insights to researchers and regulatory bodies to ensure the quality, efficacy, and safety of antihistamine products., Methods: The molecular conformation of two bilastine forms was obtained through DFT with the exchange-correlation functional M06-2X and the 6-311 + + G(d,p) basis set, and the results were compared with the experimental X-ray. The atomic coordinates were obtained directly from the crystalline structures, and charge transfer was also investigated using frontier molecular orbitals (HOMO and LUMO), and MEP map in order to evaluate the energies associated with charge transfers and regions of high electron affinity. The geometric and topological parameters and intermolecular interactions in the crystals were analyzed using Hirshfeld Surface., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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- 2024
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17. Use of carminic acid immobilized in agarose gel as a binding phase for DGT: A new approach for determinations of rare earth elements.
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Pompeu Prado Moreira LF, Geraldo de Oliveira Junior E, Borges Teixeira Zanatta M, Menegário AA, and Gemeiner H
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Recently, rare-earth elements (REEs) have attracted great interest due to their importance in several fields, such as the high-technology and medicine industries. Due to the recent intensification of the use of REEs in the world and the resulting potential impact on the environment, new analytical approaches for their determination, fractionation and speciation are needed. Diffusive gradients in thin films are a passive technique already used for sampling labile REEs, providing in situ analyte concentration, fractionation and, consequently, remarkable information on REE geochemistry. However, data based on DGT measurements until now have been based exclusively on the use of a single binding phase (Chelex-100, immobilized in APA gel). The present work proposes a new method for the determination of rare earth elements using an inductively coupled plasma‒mass spectrometry technique and a diffusive gradients in thin films (DGT) technique for application in aquatic environments. New binding gels were tested for DGT using carminic acid as the binding agent. It was concluded that acid dispersion directly in agarose gel presented the best performance, offering a simpler, faster, and greener method for measuring labile REEs compared to the existing DGT binding phase. Deployment curves obtained by immersion tests in the laboratory show that 13 REEs had linearity in their retention by the developed binding agent (retention x time), confirming the main premise of the DGT technique obeying the first Fick's diffusion law. For the first time, the diffusion coefficients were obtained in agarose gels (diffusion medium) and carminic acid immobilized in agarose as the binding phase for La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho, Er, Tm, Yb and Lu, which were 3.94 × 10
-6 , 3.87 × 10-6 , 3.90 × 10-6, 3.79 × 10-6 , 3.71 × 10-6 , 4.13 × 10-6 , 3.75 × 10-6 , 3.94 × 10-6 , 3.45 × 10-6 , 3.97 × 10-6 , 3.25 × 10-6 , 4.06 × 10-6 , and 3.50 × 10-6 cm2 s-1 , respectively. Furthermore, the proposed DGT devices were tested in solutions with different pH values (3.5, 5.0, 6.5 and 8) and ionic strengths (I = 0.005 mol L-1 , 0.01 mol L-1 , 0.05 mol L-1 and 0.1 mol L-1 - NaNO3 ). The results of these studies showed an average variation in the analyte retention for all elements at a maximum of approximately 20% in the pH tests. This variation is considerably lower than those previously reported when using Chelex resin as a binding agent, particularly for lower pH values. For the ionic strength, the maximum average variation was approximately 20% for all elements (except for I = 0.005 mol L-1 ). These results indicate the possibility of a wide range of the proposed approach to be used for in situ deployment without the use of correction based on apparent diffusion coefficients (as required for using the conventional approach). In laboratory deployments using acid mine drainage water samples (treated and untreated), it was shown that the proposed approach presents excellent accuracy compared with data obtained from Chelex resin as a binding agent., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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18. MiRNA-30d and miR-770-5p as potential clinical risk predictors of Vasoplegic Syndrome in Patients undergoing on-pump coronary artery bypass grafting.
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Vilca Mejia OA, de Souza RC, S Santos A, Meneghini B, Carvalho Silva AC, Visconde Brasil G, Oliveira Carvalho Rigaud V, Palma Dallan LR, Pinho Moreira LF, Ferreira Lisboa LA, Oliveira Dallan LA, Kalil J, Cunha-Neto E, Rodrigues Pinto Ferreira L, and Biscegli Jatene F
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- Humans, Biomarkers, Prognosis, Signal Transduction, Gene Expression Profiling, Vasoplegia genetics, MicroRNAs metabolism
- Abstract
The aims of this study were to perform pre-surgery miRNA profiling of patients who develop Vasoplegic syndrome (VS) after coronary artery bypass grafting (CABG) and identify those miRNAs that could be used as VS prognostic tools and biomarkers. The levels of 754 microRNAs (miRNAs) were measured in whole blood samples from a cohort of patients collected right before the coronary artery bypass grafting (CABG) surgery. We compared the miRNA levels of those who developed VS (VASO group) with those who did not (NONVASO group) after surgery. Six miRNAs (hsa-miR-548c-3p, -199b-5p, -383-5p -571 -183-3p, -30d-5p) were increased and two (hsa-1236-3p, and hsa-miR770-5p) were decreased in blood of VASO compared to NONVASO groups. Receiver Operating Characteristic (ROC) curve analysis revealed that a combination of the miRNAs, hsa-miR-30d-5p and hsa-miR-770-5p can be used as VS predictors (AUC = 0.9615, p < 0.0001). The computational and functional analyses were performed to gain insights into the potential role of these dysregulated miRNAs in VS and have identified the "Apelin Liver Signaling Pathway" as the canonical pathway containing the most target genes regulated by these miRNAs. The expression of the combined miRNAs hsa-miR-30d and hsa-miR-770-5p allowed the ability to distinguish between patients who could and could not develop VS, representing a potential predictive biomarker of VS., (© 2023. The Author(s).)
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- 2023
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19. An Artificial Intelligence-as-a-Service Architecture for deep learning model embodiment on low-cost devices: A case study of COVID-19 diagnosis.
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Rodrigues Moreira LF, Moreira R, Travençolo BAN, and Backes AR
- Abstract
Coronavirus Disease-2019 (COVID-19) causes Severe Acute Respiratory Syndrome-Corona Virus-2 (SARS-CoV-2) and has opened several challenges for research concerning diagnosis and treatment. Chest X-rays and computed tomography (CT) scans are effective and fast alternatives to detect and assess the damage that COVID causes to the lungs at different stages of the disease. Although the CT scan is an accurate exam, the chest X-ray is still helpful due to the cheaper, faster, lower radiation exposure, and is available in low-incoming countries. Computer-aided diagnostic systems based on Artificial Intelligence (AI) and computer vision are an alternative to extract features from X-ray images, providing an accurate COVID-19 diagnosis. However, specialized and expensive computational resources come across as challenging. Also, it needs to be better understood how low-cost devices and smartphones can hold AI models to predict diseases timely. Even using deep learning to support image-based medical diagnosis, challenges still need to be addressed once the known techniques use centralized intelligence on high-performance servers, making it difficult to embed these models in low-cost devices. This paper sheds light on these questions by proposing the Artificial Intelligence as a Service Architecture (AIaaS), a hybrid AI support operation, both centralized and distributed, with the purpose of enabling the embedding of already-trained models on low-cost devices or smartphones. We demonstrated the suitability of our architecture through a case study of COVID-19 diagnosis using a low-cost device. Among the main findings of this paper, we point out the performance evaluation of low-cost devices to handle COVID-19 predicting tasks timely and accurately and the quantitative performance evaluation of CNN models embodiment on low-cost devices., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 Elsevier B.V. All rights reserved.)
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- 2023
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20. Limited Efficacy of Adipose Stromal Cell Secretome-Loaded Skin-Derived Hydrogels to Augment Skin Flap Regeneration in Rats.
- Author
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Vriend L, van Dongen JA, Sinkunas V, Brouwer LA, Buikema HJ, Moreira LF, Gemperli R, Bongiovanni L, de Bruin A, van der Lei B, Camargo CP, and Harmsen MC
- Subjects
- Swine, Rats, Humans, Animals, Culture Media, Conditioned metabolism, Delayed-Action Preparations metabolism, Secretome, Rats, Wistar, Adipose Tissue metabolism, Stromal Cells metabolism, Hydrogels pharmacology, Pepsin A metabolism
- Abstract
Insufficient vascularization is a recurring cause of impaired pedicled skin flap healing. The administration of adipose tissue-derived stromal cells' (ASCs') secretome is a novel approach to augment vascularization. Yet, the secretome comprised of soluble factors that require a sustained-release vehicle to increase residence time. We hypothesized that administration of a hydrogel derived from decellularized extracellular matrix (ECM) of porcine skin with bound trophic factors from ASCs enhances skin flap viability and wound repair in a rat model. Porcine skin was decellularized and pepsin-digested to form a hydrogel at 37°C. Conditioned medium (CMe) of human ASC was collected, concentrated 20-fold, and mixed with the hydrogel. Sixty Wistar rats were included. A dorsal skin flap (caudal based) of 3 × 10 cm was elevated for topical application of DMEM (group I), a prehydrogel with or without ASC CMe (groups II and III), or ASC CMe (group IV). After 7, 14, and 28 days, perfusion was measured, and skin flaps were harvested for wound healing assessment and immunohistochemical analysis. Decellularized skin ECM hydrogel contained negligible amounts of DNA (11.6 ± 0.6 ng/mg), was noncytotoxic and well tolerated by rats. Irrespective of ASC secretome, ECM hydrogel application resulted macroscopically and microscopically in similar dermal wound healing in terms of proliferation, immune response, and matrix remodeling as the control group. However, ASC CMe alone increased vessel density after 7 days. Porcine skin-derived ECM hydrogels loaded with ASC secretome are noncytotoxic but demand optimization to significantly augment wound healing of skin flaps.
- Published
- 2022
- Full Text
- View/download PDF
21. Defective hematopoietic differentiation of immune aplastic anemia patient-derived iPSCs.
- Author
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Tellechea MF, Donaires FS, de Carvalho VS, Santana BA, da Silva FB, Tristão RS, Moreira LF, de Souza AF, Armenteros YM, Pereira LV, and Calado RT
- Subjects
- Bone Marrow Failure Disorders, Cell Differentiation, Hematopoietic Stem Cells pathology, Humans, Anemia, Aplastic genetics, Anemia, Aplastic pathology, Induced Pluripotent Stem Cells
- Abstract
In acquired immune aplastic anemia (AA), pathogenic cytotoxic Th1 cells are activated and expanded, driving an immune response against the hematopoietic stem and progenitor cells (HSPCs) that provokes cell depletion and causes bone marrow failure. However, additional HSPC defects may contribute to hematopoietic failure, reflecting on disease outcomes and response to immunosuppression. Here we derived induced pluripotent stem cells (iPSCs) from peripheral blood (PB) erythroblasts obtained from patients diagnosed with immune AA using non-integrating plasmids to model the disease. Erythroblasts were harvested after hematologic response to immunosuppression was achieved. Patients were screened for germline pathogenic variants in bone marrow failure-related genes and no variant was identified. Reprogramming was equally successful for erythroblasts collected from the three immune AA patients and the three healthy subjects. However, the hematopoietic differentiation potential of AA-iPSCs was significantly reduced both quantitatively and qualitatively as compared to healthy-iPSCs, reliably recapitulating disease: differentiation appeared to be more severely affected in cells from the two patients with partial response as compared to the one patient with complete response. Telomere elongation and the telomerase machinery were preserved during reprogramming and differentiation in all AA-iPSCs. Our results indicate that iPSCs are a reliable platform to model immune AA and recapitulate clinical phenotypes. We propose that the immune attack may cause specific epigenetic changes in the HSPCs that limit adequate proliferation and differentiation., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
22. Accuracy of 68 Ga-PSMA PET/CT for lymph node and bone primary staging in prostate cancer.
- Author
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Moreira LF, Mussi TC, Cunha MLD, Filippi RZ, and Baroni RH
- Subjects
- Gallium Isotopes, Gallium Radioisotopes, Humans, Lymph Nodes diagnostic imaging, Lymph Nodes pathology, Male, Neoplasm Staging, Positron Emission Tomography Computed Tomography methods, Prostatic Neoplasms pathology
- Abstract
Purpose: The aim of this study is to assess the accuracy of the
68 Ga-PSMA PET/CT for lymph nodes and bones in the primary stage of prostate cancer., Methods: A total of 126 patients who were submitted to68 Ga-PSMA PET/CT from January 2016 to February 2019 for prostate cancer staging, detection of clinically significant lesions or active surveillance were included in this study. All studies were read by 2 experienced physicians (a nuclear physician and a radiologist). The reports were made in consensus and used by one of the authors to classify the exam in positive or negative. We evaluated presence of abnormal uptake in the prostate, lymph nodes, and bone. The reference standards were histopathological confirmation, confirmatory imaging exams and/or clinical follow-up showing lesion(s) regression after specific treatment, or typical osseous metastatic lesions and highly increased PSA levels., Results: Measurement of diagnostic performance indicated a sensitivity, specificity and accuracy of 75%, 96.3%, and 90.8%, respectively, for lymph node involvement, and 90.9%, 50%, and 76.5%, respectively for metastatic bone lesions., Conclusion: This study showed high specificity and accuracy of68 Ga-PSMA PET/CT for lymph node and bone involvement in prostate cancer staging., (Copyright © 2021. Published by Elsevier Inc.)- Published
- 2022
- Full Text
- View/download PDF
23. Potential of the Compounds from Bixa orellana Purified Annatto Oil and Its Granules (Chronic ® ) against Dyslipidemia and Inflammatory Diseases: In Silico Studies with Geranylgeraniol and Tocotrienols.
- Author
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Batista MA, de Lima Teixeira Dos Santos AVT, do Nascimento AL, Moreira LF, Souza IRS, da Silva HR, Pereira ACM, da Silva Hage-Melim LI, and Carvalho JCT
- Subjects
- Bixaceae, Carotenoids, Diterpenes, Molecular Docking Simulation, Plant Extracts pharmacology, Dyslipidemias, Tocotrienols pharmacology
- Abstract
Some significant compounds present in annatto are geranylgeraniol and tocotrienols. These compounds have beneficial effects against hyperlipidemia and chronic diseases, where oxidative stress and inflammation are present, but the exact mechanism of action of such activities is still a subject of research. This study aimed to evaluate possible mechanisms of action that could be underlying the activities of these molecules. For this, in silico approaches such as ligand topology (PASS and SEA servers) and molecular docking with the software GOLD were used. Additionally, we screened some pharmacokinetic and toxicological parameters using the servers PreADMET, SwissADME, and ProTox-II. The results corroborate the antidyslipidemia and anti-inflammatory activities of geranylgeraniol and tocotrienols. Notably, some new mechanisms of action were predicted to be potentially underlying the activities of these compounds, including inhibition of squalene monooxygenase, lanosterol synthase, and phospholipase A
2 . These results give new insight into new mechanisms of action involved in these molecules from annatto and Chronic® .- Published
- 2022
- Full Text
- View/download PDF
24. BRAZILIAN GASTRIC CANCER ASSOCIATION GUIDELINES (PART 2): UPDATE ON TREATMENT.
- Author
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Barchi LC, Ramos MFKP, Dias AR, Forones NM, Carvalho MP, Castro OAP, Kassab P, Costa-Júnior WLD, Weston AC, Zilberstein B, Ferraz ÁAB, ZeideCharruf A, Brandalise A, Silva AMD, Alves B, Marins CAM, Malheiros CA, Leite CV, Bresciani CJC, Szor D, Mucerino DR, Wohnrath DR, JirjossIlias E, Martins Filho ED, PinatelLopasso F, Coimbra FJF, Felippe FEC, Tomasisch FDS, Takeda FR, Ishak G, Laporte GA, Silva HJT, Cecconello I, Rodrigues JJG, Grande JCD, Lourenço LG, Motta LMD, Ferraz LR, Moreira LF, Lopes LR, Toneto MG, Mester M, Rodrigues MAG, Franciss MY, AdamiAndreollo N, Corletta OC, Yagi OK, Malafaia O, Assumpção PP, Savassi-Rocha PR, Colleoni Neto R, Oliveira RJ, AissarSallun RA, Weschenfelder R, Oliveira SCV, Abreu TB, Castria TB, Ribeiro Junior U, Barra W, and Freitas Júnior WR
- Subjects
- Brazil, Consensus, Gastrectomy, Humans, Lymph Node Excision, Stomach Neoplasms surgery
- Abstract
Background: : The II Brazilian Consensus on Gastric Cancer of the Brazilian Gastric Cancer Association BGCA (Part 1) was recently published. On this occasion, countless specialists working in the treatment of this disease expressed their opinion in the face of the statements presented., Aim: : To present the BGCA Guidelines (Part 2) regarding indications for surgical treatment, operative techniques, extension of resection and multimodal treatment., Methods: To formulate these guidelines, the authors carried out an extensive and current review regarding each declaration present in the II Consensus, using the Medline/PubMed, Cochrane Library and SciELO databases initially with the following descriptors: gastric cancer, gastrectomy, lymphadenectomy, multimodal treatment. In addition, each statement was classified according to the level of evidence and degree of recommendation., Results: : Of the 43 statements present in this study, 11 (25,6%) were classified with level of evidence A, 20 (46,5%) B and 12 (27,9%) C. Regarding the degree of recommendation, 18 (41,9%) statements obtained grade of recommendation 1, 14 (32,6%) 2a, 10 (23,3%) 2b e one (2,3%) 3., Conclusion: : The guidelines complement of the guidelines presented here allows surgeons and oncologists who work to combat gastric cancer to offer the best possible treatment, according to the local conditions available.
- Published
- 2021
- Full Text
- View/download PDF
25. BRAZILIAN GASTRIC CANCER ASSOCIATION GUIDELINES (PART 1): AN UPDATE ON DIAGNOSIS, STAGING, ENDOSCOPIC TREATMENT AND FOLLOW-UP.
- Author
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Barchi LC, Ramos MFKP, Yagi OK, Mucerino DR, Bresciani CJC, Ribeiro JÚnior U, Andreollo NA, AssumpÇÃo PP, Weston AC, Colleoni Neto R, Zilberstein B, Ferraz ÁAB, Charruf AZ, Dias AR, Brandalise A, Silva AMD, Alves B, Malheiros CA, Marins CAM, Leite CV, Szor D, Wohnrath DR, Ilias EJ, Martins Filho ED, Lopasso FP, Coimbra FJF, Felippe FEC, Tomasisch FDS, Takeda FR, Ishak G, Laporte GA, Silva HJT, Cecconello I, Rodrigues JJG, Grande JCD, Lourenço LG, Motta LMD, Ferraz LR, Moreira LF, Lopes LR, Toneto MG, Mester M, Rodrigues MAG, Carvalho MP, Franciss MY, Forones NM, Corletta OC, Castro OAP, Malafaia O, Kassab P, Savassi-Rocha PR, Oliveira RJ, Sallun RAA, Weschenfelder R, Oliveira SCV, Abreu TB, Castria TB, Barra W, Costa Júnior WLD, and Freitas Júnior WR
- Subjects
- Brazil, Consensus, Follow-Up Studies, Humans, Endoscopy, Digestive System, Neoplasm Staging, Stomach Neoplasms diagnosis, Stomach Neoplasms surgery
- Abstract
Background: The II Brazilian Consensus on Gastric Cancer by the Brazilian Gastric Cancer Association (ABCG) was recently published. On this occasion, several experts in gastric cancer expressed their opinion before the statements presented., Aim: To present the ABCG Guidelines (part 1) regarding the diagnosis, staging, endoscopic treatment and follow-up of gastric cancer patients., Methods: To forge these Guidelines, the authors carried out an extensive and current review regarding each statement present in the II Consensus, using the Medline/PubMed, Cochrane Library and SciELO databases with the following descriptors: gastric cancer, staging, endoscopic treatment and follow-up. In addition, each statement was classified according to the level of evidence and degree of recommendation., Results: Of the 24 statements, two (8.3%) were classified with level of evidence A, 11 (45.8%) with B and 11 (45.8%) with C. As for the degree of recommendation, six (25%) statements obtained grade of recommendation 1, nine (37.5%) recommendation 2a, six (25%) 2b and three (12.5%) grade 3., Conclusion: The guidelines presented here are intended to assist professionals working in the fight against gastric cancer with relevant and current information, granting them to be applied in the daily medical practice.
- Published
- 2020
- Full Text
- View/download PDF
26. Raltegravir versus efavirenz in antiretroviral-naive pregnant women living with HIV (NICHD P1081): an open-label, randomised, controlled, phase 4 trial.
- Author
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João EC, Morrison RL, Shapiro DE, Chakhtoura N, Gouvèa MIS, de Lourdes B Teixeira M, Fuller TL, Mmbaga BT, Ngocho JS, Njau BN, Violari A, Mathiba R, Essack Z, Pilotto JHS, Moreira LF, Rolon MJ, Cahn P, Prommas S, Cressey TR, Chokephaibulkit K, Werarak P, Laimon L, Hennessy R, Frenkel LM, Anthony P, Best BM, Siberry GK, and Mirochnick M
- Subjects
- Adult, Alkynes, Anti-HIV Agents adverse effects, Benzoxazines adverse effects, Cyclopropanes, Drug Therapy, Combination, Female, HIV Infections prevention & control, Humans, Infant, Newborn, Infectious Disease Transmission, Vertical prevention & control, Lamivudine therapeutic use, Outcome Assessment, Health Care, Pregnancy, Raltegravir Potassium adverse effects, Viral Load drug effects, Young Adult, Zidovudine therapeutic use, Anti-HIV Agents therapeutic use, Benzoxazines therapeutic use, HIV Infections drug therapy, HIV Integrase Inhibitors therapeutic use, Pregnancy Complications, Infectious drug therapy, Raltegravir Potassium therapeutic use
- Abstract
Background: Although antiretroviral regimens containing integrase inhibitors rapidly suppress HIV viral load in non-pregnant adults, few published data from randomised controlled trials have compared the safety and efficacy of any integrase inhibitor to efavirenz when initiated during pregnancy. We compared safety and efficacy of antiretroviral therapy with either raltegravir or efavirenz in late pregnancy., Methods: An open-label, randomised controlled trial was done at 19 hospitals and clinics in Argentina, Brazil, South Africa, Tanzania, Thailand, and the USA. Antiretroviral-naive pregnant women (20-<37 weeks gestation) living with HIV were assigned to antiretroviral regimens containing either raltegravir (400 mg twice daily) or efavirenz (600 mg each night) plus lamivudine 150 mg and zidovudine 300 mg twice daily (or approved alternative backbone regimen), using a web-based, permuted-block randomisation stratified by gestational age and backbone regimen. The primary efficacy outcome was plasma HIV viral load below 200 copies per mL at (or near) delivery. The primary efficacy analysis included all women with a viral load measurement at (or near) delivery who had viral load of at least 200 copies per mL before treatment and no genotypic resistance to any study drugs; secondary analyses eliminated these exclusion criteria. The primary safety analyses included all women who received study drug, and their infants. This trial is registered with Clinicaltrials.gov, number NCT01618305., Findings: From Sep 5, 2013, to Dec 11, 2018, 408 women were enrolled (206 raltegravir, 202 efavirenz) and 394 delivered on-study (200 raltegravir, 194 efavirenz); 307 were included in the primary efficacy analysis (153 raltegravir, 154 efavirenz). 144 (94%) women in the raltegravir group and 129 (84%) in the efavirenz group met the primary efficacy outcome (absolute difference 10%, 95% CI 3-18; p=0·0015); the difference primarily occurred among women enrolling later in pregnancy (interaction p=0·040). Frequencies of severe or life-threatening adverse events were similar among mothers (30% in each group; 61 raltegravir, 59 efavirenz) and infants (25% in each group; 50 raltegravir, 48 efavirenz), with no treatment-related deaths., Interpretation: Our findings support major guidelines. The integrase inhibitor dolutegravir is currently a preferred regimen for the prevention of perinatal HIV transmission with raltegravir recommended as a preferred or alternative integrase inhibitor for pregnant women living with HIV., Funding: Eunice Kennedy Shriver National Institute of Child Health and Human Development and National Institute of Allergy and Infectious Diseases., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
27. Transcatheter mitral valve-in-valve implantation: reports of the first 50 cases from a Latin American Centre.
- Author
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da Costa LPN, Palma JH, Barbosa Ribeiro H, Sampaio RO, Viotto G, Medeiros Santos R, Freitas Tenório D, Saito VT, Egypto Rosa VE, Pinho Moreira LF, Tarasoutchi F, Pomerantzeff PM, and Biscegli Jatene F
- Subjects
- Echocardiography, Female, Heart Valve Diseases diagnosis, Humans, Latin America, Male, Middle Aged, Mitral Valve diagnostic imaging, Prospective Studies, Prosthesis Design, Time Factors, Treatment Outcome, Heart Valve Diseases surgery, Heart Valve Prosthesis, Heart Valve Prosthesis Implantation methods, Mitral Valve surgery
- Abstract
Objectives: Our goal was to analyse the initial results of the first 50 transapical transcatheter mitral valve-in-valve procedures performed in a single Latin American centre., Methods: A prospective, single centre, database analysis was conducted to evaluate immediate, 30-day and 1-year postoperative results of 50 consecutive patients who had a transcatheter mitral valve-in-valve procedure from May 2015 to June 2018. All patients were operated on in a hybrid operating room and received a balloon-expandable valve via the transapical approach. Preoperative and postoperative characteristics were analysed and compared between the first 25 and the second 25 patients to evaluate the impact of the learning curve. Twenty patients had a follow-up examination at 1 year., Results: There was a 98% device success rate. The patients had a mean age of 64.8 years; 72% were women; 80% were in New York Heart Association functional class ≥III preoperatively; and 36% of the procedures were urgent. The mean Society of Thoracic Surgeons scores and EuroSCORE II were 8.3% and 12.4%, respectively. Patients had a median of 2 previous operations; valve durability was 12.1 years; and 64% mitral valve disease of rheumatic fever aetiology. Echocardiography showed decreases in the maximum and mean mitral gradients from 23.5 to 14.6 and 11.5 to 6.4 mmHg postoperatively; the overall mean hospitalization period was 15 days. The overall mortality rate at 30 days was 14%, with 1 intraprocedural death. Further subanalyses between the first and the second half of the cases showed a drop in the mortality rate from 20% to 8% (P < 0.01)., Conclusion: The transcatheter mitral valve-in-valve procedure was shown to be a safe and effective procedure to treat bioprosthetic dysfunction, with potential benefits in patients with rheumatic disease., (© The Author(s) 2019. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
28. Electrochemical sensor based on reduced graphene oxide and molecularly imprinted poly(phenol) for d-xylose determination.
- Author
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Pompeu Prado Moreira LF, Buffon E, and Stradiotto NR
- Abstract
The present work reports the development of an electrochemical sensor based on molecularly imprinted polymer for the determination of d-xylose. This is the first report of its kind in the literature. The sensor was prepared through the modification of a glassy carbon electrode with reduced graphene oxide and molecularly imprinted poly(phenol) film. The use of graphene oxide and molecularly imprinted poly(phenol) film led to remarkable improvements in the sensor sensitivity and selectivity, respectively. The electrode was characterized by several techniques, including cyclic voltammetry, differential pulse voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, atomic force microscopy and RAMAN spectroscopy. The proposed sensor presented linear responses ranging from 1.0 × 10
-13 to 1.0 × 10-11 mol L-1 . The amperometric sensitivity, limit of detection, and limit of quantification obtained were 6.7 × 105 A L mol-1 ; 8.0 × 10-14 mol L-1 and 2.7 × 10-13 mol L-1 (n = 3), respectively. The proposed analytical method was successfully applied in sugarcane bagasse, which is known to contain large amounts of d-xylose and other structurally similar molecules in its composition. The chemical composition of sugarcane bagasse makes this biomass suitable for evaluating the ability of the sensor to specifically detect the target molecule. Mean recoveries obtained in the analysis ranged from 95.4 to 105.0%; this indicates that the proposed method has good accuracy when applied toward the determination of d-xylose., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
29. NUTRIC score use around the world: a systematic review.
- Author
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Reis AMD, Fructhenicht AVG, and Moreira LF
- Subjects
- Humans, Critical Care, Critical Illness, Nutrition Assessment, Risk Assessment
- Abstract
Objective: To collect data on the use of The Nutrition Risk in Critically Ill (NUTRIC) score., Methods: A systematic literature search was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Reviews, abstracts, dissertations, protocols and case reports were excluded from this review; to be included in the review, studies needed to specifically evaluate the NUTRIC score and to have been published in English, Spanish or Portuguese., Results: We included 12 (0.8%) studies from our search in this review. Ten studies (83.3%) were observational, 1 was a pilot study (8.3%) and 1 was a randomized control trial (8.3%). All of the included studies (100%) chose not to use IL-6 and considered a high nutritional risk cutoff point ≥ 5. There were 11 (91.7%) English language studies versus 1 (8.3%) Spanish language study. Mechanical ventilation and a high NUTRIC score were significantly correlated in four studies. The association between intensive care unit or hospital length of stay and nutritional high risk was significant in three studies. Seven studies found a statistically significant association between the NUTRIC score and mortality., Conclusion: The NUTRIC score is related to clinical outcomes, such as length of hospital stay, and is appropriate for use in critically ill patients in intensive care units.
- Published
- 2019
- Full Text
- View/download PDF
30. Telomere dynamics and hematopoietic differentiation of human DKC1-mutant induced pluripotent stem cells.
- Author
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Donaires FS, Alves-Paiva RM, Gutierrez-Rodrigues F, da Silva FB, Tellechea MF, Moreira LF, Santana BA, Traina F, Dunbar CE, Winkler T, and Calado RT
- Subjects
- Cells, Cultured, Cellular Reprogramming, Dyskeratosis Congenita genetics, Dyskeratosis Congenita pathology, Fibroblasts cytology, Humans, Induced Pluripotent Stem Cells cytology, Induced Pluripotent Stem Cells metabolism, Karyotype, Mutation, Telomerase genetics, Telomerase metabolism, Telomere Shortening, Cell Cycle Proteins genetics, Cell Differentiation, Hematopoiesis, Nuclear Proteins genetics, Telomere metabolism
- Abstract
Telomeropathies are a group of phenotypically heterogeneous diseases molecularly unified by pathogenic mutations in telomere-maintenance genes causing critically short telomeres. X-linked dyskeratosis congenita (DC), the prototypical telomere disease, manifested with ectodermal dysplasia, cancer predisposition, and severe bone marrow failure, is caused by mutations in DKC1, encoding a protein responsible for telomerase holoenzyme complex stability. To investigate the effects of pathogenic DKC1 mutations on telomere repair and hematopoietic development, we derived induced pluripotent stem cells (iPSCs) from fibroblasts of a DC patient carrying the most frequent mutation: DKC1 p.A353V. Telomeres eroded immediately after reprogramming in DKC1-mutant iPSCs but stabilized in later passages. The telomerase activity of mutant iPSCs was comparable to that observed in human embryonic stem cells, and no evidence of alternative lengthening of telomere pathways was detected. Hematopoietic differentiation was carried out in DKC1-mutant iPSC clones that resulted in increased capacity to generate hematopoietic colony-forming units compared to controls. Our study indicates that telomerase-dependent telomere maintenance is defective in pluripotent stem cells harboring DKC1 mutation and unable to elongate telomeres, but sufficient to maintain cell proliferation and self-renewal, as well as to support the primitive hematopoiesis, the program that is recapitulated with our differentiation protocol., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2019
- Full Text
- View/download PDF
31. Predicting factors of postoperative complications in appendectomies.
- Author
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Moreira LF, Garbin HI, Da-Natividade GR, Silveira BV, and Xavier TV
- Subjects
- Acute Disease, Adult, Age Factors, Appendectomy methods, Female, Humans, Laparoscopy, Length of Stay, Male, Middle Aged, Retrospective Studies, Risk Factors, Severity of Illness Index, Appendectomy adverse effects, Appendicitis surgery, Postoperative Complications
- Abstract
Objective: to evaluate the main risk factors for postoperative complications in patients undergoing appendectomy for acute appendicitis., Methods: we retrospectively analyzed 1241 patients undergoing open or laparoscopic appendectomy. Patients were allocated to four groups: Group 1, without postoperative complications, and Groups 2, 3, and 4, with postoperative complications defined according to their severity, following Clavien-Dindo classification (I, II, and ≥III, respectively)., Results: patients aged ≥38.5 years had more severe complications (p<0.0001). Patients in Group 1, without postoperative complications, had, for the most part, a laparoscopic approach. Groups 2, 3, and 4 underwent, predominantly, to an open surgery (p<0.0001). In cases of acute appendicitis, the odds ratio (OR) for complications was 3.09, 3.04, and 12.41 for Groups 2, 3, and 4, respectively (p<0.0001). Anesthetic risk, duration of the procedure, and length of hospital stay were related to a higher risk and severity of complications., Conclusion: the main predicting factors of postoperative complications in patients operated for acute appendicitis were: age ≥38.5 years, conventional or open surgical access, complicated appendicitis, ASA≥2, and surgical time >77 minutes.
- Published
- 2018
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32. Clinical, laboratory and densitometric comparison of patients with coxarthrosis and femoral neck fractures.
- Author
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Spinelli LF, Pagnussato F, Ribeiro TA, Guareze FS, Feder MG, Macedo CAS, Moreira LF, and Galia CR
- Subjects
- Aged, Aged, 80 and over, Body Mass Index, Bone Density, Cross-Sectional Studies, Female, Femoral Neck Fractures blood, Humans, Male, Middle Aged, Osteoarthritis, Hip blood, Treatment Outcome, Arthroplasty, Replacement, Hip, Biomarkers blood, Femoral Neck Fractures surgery, Osteoarthritis, Hip surgery
- Abstract
Objective: to compare clinical, laboratory and densitometric data from patients with osteoarthrosis and femoral neck fractures., Methods: we conducted a cross-sectional study of patients with femoral neck fracture and hip osteoarthrosis submitted to hip arthroplasty. We collected clinical, laboratory and densitometric data., Results: we included 53 patients, 22 with femoral neck fractures and 31 with osteoarthrosis. Patients with femoral neck fractures were older than patients with osteoarthrosis, with lower BMI values, bone mineral density and palmar grip strength (sarcopenic patients), being more neurologically impaired and presenting a worse ASA score. Among the various biochemical parameters analyzed, we found statistically significant differences in total serum calcium, ionized calcium, vitamin D, free thyroxine, erythrocytes, hemoglobin, hematocrit, total white blood cells, neutrophils, lymphocytes and creatinine between the two groups. Other hormones analyzed and biochemical parameters did not differ significantly, although they showed trends between the two groups., Conclusion: patients with femoral neck fractures are older than patients with osteoarthrosis, have a lower weight and BMI, are more debilitated, many with anemia and reduced bone mass, and have a significant decrease in total calcium, ionized calcium, vitamin D and creatinine and a significant increase in free thyroxine.
- Published
- 2018
- Full Text
- View/download PDF
33. Complement System as a Target for Therapies to Control Liver Regeneration/Damage in Acute Liver Failure Induced by Viral Hepatitis.
- Author
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Melgaço JG, Veloso CE, Pacheco-Moreira LF, Vitral CL, and Pinto MA
- Subjects
- Adolescent, Adult, Apoptosis, Cell Line, Cell Proliferation, Child, Child, Preschool, Complement System Proteins therapeutic use, Female, Hepatitis, Viral, Human complications, Humans, Immunity, Innate, Immunomodulation, Infant, Liver pathology, Liver virology, Liver Failure, Acute etiology, Male, Molecular Targeted Therapy, Young Adult, Complement System Proteins metabolism, Hepatitis, Viral, Human therapy, Liver immunology, Liver Failure, Acute therapy, Liver Regeneration physiology
- Abstract
The complement system plays an important role in innate immunity inducing liver diseases as well as signaling immune cell activation in local inflammation regulating immunomodulatory effects such as liver damage and/or liver regeneration. Our aim is to evaluate the role of complement components in acute liver failure (ALF) caused by viral hepatitis, involving virus-induced ALF in human subjects using peripheral blood, samples of liver tissues, and ex vivo assays. Our findings displayed low levels of C3a in plasma samples with high frequency of C3a, C5a, and C5b/9 deposition in liver parenchyma. Meanwhile, laboratory assays using HepG2 (hepatocyte cell line) showed susceptibility to plasma samples from ALF patients impairing in vitro cell proliferation and an increase in apoptotic events submitting plasma samples to heat inactivation. In summary, our data suggest that the complement system may be involved in liver dysfunction in viral-induced acute liver failure cases using ex vivo assays. In extension to our findings, we provide insights into future studies using animal models for viral-induced ALF, as well as other associated soluble components, which need further investigation.
- Published
- 2018
- Full Text
- View/download PDF
34. Spodoptera albula susceptibility to Bacillus thuringiensis-based biopesticides.
- Author
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Gonçalves KC, Boiça Júnior AL, Duarte RT, Moreira LF, do Nascimento J, and Polanczyk RA
- Subjects
- Animals, Biological Control Agents, Virulence, Bacillus thuringiensis pathogenicity, Pest Control, Biological methods, Spodoptera parasitology
- Abstract
Single concentration and virulence (mean lethal concentration) bioassays were performed to evaluate the susceptibility of S. albula second instar larvae to seven Bacillus thuringiensis-based biopesticides. Bioassays were conducted using three replicates and repeated three times at 25 °C, 70 ± 10% RH, and a 12:12 (light/dark) photoperiod; mortality was recorded seven days after treatment. The results were subjected to a Tukey's test and Probit analysis. Agree, DiPel SC, and XenTari achieved mortality rates of up to 80%, with the first of these being the most virulent against S. albula. Different Dipel formulations showed different degrees of larvicidal activity., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
35. Role of BI-RADS Ultrasound Subcategories 4A to 4C in Predicting Breast Cancer.
- Author
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Spinelli Varella MA, Teixeira da Cruz J, Rauber A, Varella IS, Fleck JF, and Moreira LF
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Biopsy, Large-Core Needle, Breast Neoplasms pathology, Child, Cross-Sectional Studies, Female, Humans, Middle Aged, Predictive Value of Tests, Sensitivity and Specificity, Young Adult, Breast Neoplasms diagnostic imaging, Ultrasonography, Mammary standards, Ultrasonography, Mammary statistics & numerical data
- Abstract
Background: The Breast Imaging Reporting and Data System (BI-RADS) ultrasound (US) categorization revised in 2013 by the American College of Radiology resulted in unquestionable standardization of reports and confirmed category 3 and 5 as benign and malignant lesions, respectively. In contrast, suspected images (category 4) have subcategorization criteria, although theses have been detailed difficult to apply. The aim of the present study was to determine the role of the US 4A to 4C BI-RADS subcategories in predicting malignancy., Patients and Methods: We performed a cross-sectional study of diagnostic tests to estimate the performance of the US BI-RADS categorization to clearly differentiate benign from malignant lesions. A total of 975 US examinations performed at the Hospital Femina, Grupo Hospitalar Conceição teaching hospitals from January 2012 through March 2015 were included in the present study. The US BI-RADS lexicon was used to classify the examination findings. Suspicious lesions underwent core needle biopsy, and the US and histology reports were compared to determine the performance using receiver operating characteristic curves., Results: Overall, the BI-RADS US categorization showed good discriminating accuracy with a receiver operating characteristic curve of 91% (95% confidence interval [CI], 88%-93%). However, BI-RADS subcategory 4b had a positive predictive value of 25% (95% CI, 20%-31%) and subcategory 4A had a positive predictive value of only 6% (95% CI, 3.5%-9.8%)., Conclusion: Our results have shown that US BI-RADS subcategories 4A and 4B are clearly unfit for use in screening tests, because they cannot rule out the need for biopsy. Therefore, management will not be improved by subcategorizing category 4, because all suspicious lesions will still require definite biopsy., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2018
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36. Use of dietary fibers in enteral nutrition of critically ill patients: a systematic review.
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Reis AMD, Fruchtenicht AV, Loss SH, and Moreira LF
- Subjects
- Critical Care methods, Critical Illness, Diarrhea etiology, Diarrhea prevention & control, Dietary Fiber adverse effects, Enteral Nutrition adverse effects, Humans, Length of Stay, Nutritional Requirements, Dietary Fiber administration & dosage, Enteral Nutrition methods, Intensive Care Units
- Abstract
To meet the nutritional requirements of patients admitted to intensive care units, it is necessary to establish a diet schedule. Complications associated with enteral nutrition by tube feeding are not uncommon and may reduce the delivery of required nutrient to patients in intensive care units. Research on the osmolality, fat content, caloric intensity and fiber content of formulas are under way, and a substantial number of studies have focused on fiber content tolerability or symptom reduction. We conducted a systematic review of dietary fiber use and safety in critically ill patients in 8 studies based on diarrhea, other gastrointestinal symptoms (abdominal distension, gastric residual volume, vomiting and constipation), intestinal microbiota, length of stay in the intensive care unit and death. We discussed the results reported in the scientific literature and current recommendations. This contemporary approach demonstrated that the use of soluble fiber in all hemodynamically stable, critically ill patients is safe and should be considered beneficial for reducing the incidence of diarrhea in this population.
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- 2018
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37. CD117 Expression in Squamous Cell Carcinoma of the Oesophagus.
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Moreira LF, Maino MM, Garbin HI, DA Natividade GR, Volkweis BS, and Kulczynski JU
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- Adult, Aged, Carcinoma, Squamous Cell pathology, Case-Control Studies, Esophageal Neoplasms pathology, Female, Humans, Immunohistochemistry, Male, Middle Aged, Retrospective Studies, Survival Analysis, Carcinoma, Squamous Cell immunology, Esophageal Neoplasms immunology, Proto-Oncogene Proteins c-kit metabolism
- Abstract
Background/aim: Identification of changes in specific genes may help new attempts in finding targeted therapy for oesophageal cancer which still has a very poor prognosis. The aim of this study was to investigate CD117 expression in squamous cell carcinoma of the oesophagus (SCCO)., Materials and Methods: A preliminary study was performed for CD177 immunoreactivity using a monoclonal antibody against CD117 on 27 SCCO specimens from patients who underwent surgical resection. Specimens of oesophageal mucosa obtained from 10 healthy individuals were studied as a control group., Results: Most patients had TNM American Joint Committee on Cancer stage IIb or III SCCO and mean overall survival was 21 (range=2-72) months. Cytoplasmic membrane CD117 immunoreactivity was demonstrated in only four (15%) out of 27 tumours and in none of the controls., Conclusion: Although immunohistochemical expression of CD117 was higher than previously demonstrated, the lack of expression does not warranty further use in targeted therapy of SCCO., (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
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- 2018
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38. PROGNOSTIC VALUE OF CARCINOEMBRYONIC ANTIGEN LEVELS IN TRANSOPERATIVE PERITONEAL LAVAGE IN PATIENTS WITH GASTRIC CANCER.
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Alves LB, Tsukazan MT, Serafim AE, Mendoza R, Padoin AV, Baú PC, and Moreira LF
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Intraoperative Care, Male, Middle Aged, Prognosis, Stomach Neoplasms immunology, Stomach Neoplasms mortality, Survival Rate, Biomarkers, Tumor analysis, Carcinoembryonic Antigen analysis, Peritoneal Lavage, Stomach Neoplasms surgery
- Abstract
Background: The carcinoembryonic antigen level in peritoneal lavage has been showing to be a reliable prognostic factor in gastric cancer., Aim: To identify any association between carcinoembryonic antigen level in peritoneal lavage, in gastric cancer patients, with mortality, peritoneal recurrence, tumor relapse or other prognostic factors., Methods: In total, 30 patients (22 men, 8 women; median age 66 years) with resectable gastric cancer (mainly stage III and IV) were studied. Carcinoembryonic antigen level in peritoneal lavage was detected at operation by immunocytochemical method and a level over 210 ng/g of protein was considered as positive., Results: There were detected 10 positive cases (33.3%) of plCEA levels. These levels were associated with mortality, RR: 2.1 (p=0.018); peritoneal recurrence, OR: 9.0 (p=0.015); and relapse or gastric cancer progression, OR: 27.0 (p=0.001)., Conclusion: Increased levels of plCEA fairly predicts mortality, peritoneal recurrence tumor relapse or cancer progression.
- Published
- 2018
- Full Text
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39. Score of "eat-ability" as a predictor of malnutrition in patients with gastrointestinal tract cancer: a pilot study.
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Dias Barreiro T, Guidi Saueressig M, Brum Kabke G, Ferreira PK, Gonçalves Fruchtenicht AV, Campos Corleta O, and Moreira LF
- Subjects
- Adult, Aged, Aged, 80 and over, Cross-Sectional Studies, Deglutition Disorders etiology, Deglutition Disorders psychology, Feeding and Eating Disorders etiology, Feeding and Eating Disorders psychology, Female, Gastrointestinal Neoplasms mortality, Humans, Male, Malnutrition mortality, Middle Aged, Nutritional Status, Outpatients, Pilot Projects, Prospective Studies, Weight Loss, Eating psychology, Gastrointestinal Neoplasms complications, Gastrointestinal Neoplasms psychology, Malnutrition etiology, Malnutrition psychology
- Abstract
Introduction: decreased food intake, loss of appetite, and dysphagia are relevant symptoms in patients with gastrointestinal tract (GIT) cancer. However, these symptoms have been isolated or indirectly assessed when comprising quality of life questionnaires or risk assessment tools., Objective: to determine whether a combined assessment of dysphagia, appetite and food intake may be used as a parameter of eat-ability (food capacity) in patients with GIT cancer., Methods: a cross-sectional pilot study on 41 patients with GIT cancer were evaluated using a score for "eat-ability"(SEA) as compared to the Patient Generated Subjective Global Assessment(PG-SGA), anthropometry and laboratory profile., Results: eleven (27%) patients had full eat-ability(SEA 0), three (7%) had moderate (SEA 1) and 27 (66%) had poor (SEA ≥ 2) eat-ability, which were significantly different, between upper and lower GIT tumors (p ≤ 0.05). By ROC curves, SEA 1 and ≥ 2 showed an 80% for both sensibility (95% CI: 0.48-0.95) and specificity (95% CI: 0.63-0.91) to PG-SGA (A and B), with an area under curve (AUC) of 0.79 (95% CI: 0.64-0.95) (p = 0.006). Patients with SEA ≥ 2 had a significant weight loss within three (p = 0.001) and six months (p < 0.001) when compared to patients with SEA 0 and 1. Mortality was also significantly higher (p = 0.01) among patients with critical food capacity by SEA (77%) in severely malnourished patients by PG-SGA (84%)., Conclusion: by combining food intake, dysphagia and appetite assessment altogether, a reliable score clearly demonstrated compromised eating capacity affecting nutritional status of patients with GIT tumors at a higher risk for death.
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- 2018
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40. ADDUCTOR POLLICIS MUSCLE THICKNESS AND PREDICTION OF POSTOPERATIVE MORTALITY IN PATIENTS WITH STOMACH CANCER.
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Poziomyck AK, Corleta OC, Cavazzola LT, Weston AC, Lameu EB, Coelho LJ, and Moreira LF
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Organ Size, Postoperative Period, Prognosis, Prospective Studies, Thumb, Muscle, Skeletal anatomy & histology, Nutrition Assessment, Stomach Neoplasms mortality, Stomach Neoplasms surgery
- Abstract
Background: Malnutrition is very prevalent in patients with gastric cancer and increases the risk of morbidity and mortality. Adductor pollicis muscle thickness (APMT) appears as an important objective, quick, inexpensive and noninvasive measure to assess the muscle compartment., Aim: To compare APMT and other nutritional assessment methods and to correlate these methods with postoperative mortality., Methods: Forty-four patients, 29 men and 15 women, mean age of 63±10.2 and ranging from 34-83 years, who underwent nine (20.5%) partial and 34 (77.3%) total gastrectomies due to stomach cancer (stage II to IIIa) were preoperatively assessed by Patient Generated Subjective Global Assessment (PG-SGA), anthropometry and laboratorial profile., Results: APMT better predicted death (p<0.001) on both, dominant and non-dominant hand, and well correlated with albumin (p=0.039) and PG-SGA (p=0.007)., Conclusion: APMT clearly allowed to determine malnutrition and to predict risk of death in patients with gastric cancer.
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- 2018
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41. Inflammatory and nutritional statuses of patients submitted to resection of gastrointestinal tumors.
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Fruchtenicht AVG, Poziomyck AK, Reis AMD, Galia CR, Kabke GB, and Moreira LF
- Subjects
- Female, Gastrointestinal Neoplasms complications, Gastrointestinal Neoplasms immunology, Gastrointestinal Neoplasms mortality, Humans, Inflammation etiology, Male, Middle Aged, Prognosis, Prospective Studies, Gastrointestinal Neoplasms surgery, Nutritional Status
- Abstract
Objective: to evaluate the association between the nutritional and the inflammatory statuses of patients with cancer of the gastrointestinal tract undergoing surgical resection and to identify predictors of mortality in these patients., Methods: we conducted a prospective study of 41 patients with gastrointestinal tract cancer submitted to surgery between October 2012 and December 2014. We evaluated the nutritional status by subjective and objective methods. We assessed the inflammatory response and prognosis using the modified Glasgow Prognostic Score (mGPS), Neutrophil/Lymphocyte Ratio (NLR), Onodera Prognostic Nutritional Index (mPNI), Inflammatory-Nutritional Index (INI) and C-Reactive Protein/Albumin ratio (mPINI)., Results: half of the patients were malnourished and 27% were at nutritional risk. There was a positive association between the percentage of weight loss (%WL) and the markers NLR (p=0.047), mPINI (p=0.014) and INI (p=0.015). Serum albumin levels (p=0.015), INI (p=0.026) and mPINI (p=0.026) were significantly associated with the PG-SGA categories. On multivariate analysis, albumin was the only inflammatory marker independently related to death (p=0.004)., Conclusion: inflammatory markers were significantly associated with malnutrition, demonstrating that the higher the inflammatory response, the worse the PG-SGA (B and C) scores and the higher the %WL in these patients. However, further studies aimed at improving surgical outcomes and determining the role of these markers as predictors of mortality are required.
- Published
- 2018
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42. Biomarkers as predictors of mortality in critically ill patients with solid tumors.
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Reis AMD, Fruchtenicht AVG, Athaydes LC, Loss S, and Moreira LF
- Subjects
- Cross-Sectional Studies, Female, Humans, Intensive Care Units statistics & numerical data, Male, Middle Aged, Neoplasms blood, Neoplasms pathology, Predictive Value of Tests, Prognosis, Prospective Studies, Biomarkers, Tumor blood, Critical Illness mortality, Neoplasms mortality
- Abstract
Biochemical markers produced by the affected organ or body in response to disease have gained high clinical value due to assess disease development and being excellent predictors of morbidity and mortality. The aim of this study is to analyze different biochemical markers in critically cancer patients and to determine which of them can be used as predictors of mortality. This is a prospective, cross-sectional study conducted at a University Hospital in Porto Alegre - RS. Screening was done to include patients in the study. Serum biochemical markers obtained in the first 24 hours of Intensive Care Unit hospitalization were analyzed. A second review of medical records occurred after three months objected to identify death or Unit discharged. A sample of 130 individuals was obtained (control group n = 65, study group n = 65). In the multivariate model, serum magnesium values OR = 3.97 (1.17; 13.5), presence of neoplasia OR = 2.68 (95% CI 1.13; 6.37) and absence of sepsis OR = 0.31 (95% CI 0.12; 0.79) were robust predictors of mortality. The association of solid tumors, sepsis presence and alteration in serum magnesium levels resulted in an increased chance of mortality in critically ill patients.
- Published
- 2017
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43. Nutritional assessment methods as predictors of postoperative mortality in gastric cancer patients submitted to gastrectomy.
- Author
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Poziomyck AK, Cavazzola LT, Coelho LJ, Lameu EB, Weston AC, and Moreira LF
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Postoperative Period, Prospective Studies, Gastrectomy, Nutrition Assessment, Stomach Neoplasms mortality, Stomach Neoplasms surgery
- Abstract
Objectives: to determine the nutritional evaluation method that best predicts mortality in 90 days of patients submitted to gastrectomy for gastric cancer., Methods: we conducted a prospective study with 44 patients with gastric cancer, stages II to IIIa, of whom nine were submitted to partial gastrectomy, 34 to total gastrectomy, and one to esophago-gastrectomy. All patients were nutritionally evaluated through the same protocol, up to 72h after hospital admission. The parameters used were Patient-Generated Subjective Global Assessment (PGSGA), classical anthropometry, current weight and height, percentage of weight loss (%WL) and body mass index (BMI). We also measured the thickness of the thumb adductor muscle (TAM) in both hands, dominant hand (TAMD) and non-dominant hand (TAMND), as well as the calculated the prognostic nutritional index (PNI). The laboratory profile included serum levels of albumin, erythrocytes, hemoglobin, hematocrit, leukocytes, and total lymphocytes count (TLC)., Results: of the 44 patients studied, 29 (66%) were malnourished by the subjective method, 15 being grade A, 18 grade B and 11 grade C. Cases with PGSGA grade B and TAMD 10.2±2.9 mm were significantly associated with higher mortality. The ROC curves (95% confidence interval) of both PGSGA and TAMD thickness reliably predicted mortality at 30 and 90 days. No laboratory method allowed predicting mortality at 90 days., Conclusion: PGSGA and the TAMD thickness can be used as preoperative parameters for risk of death in patients undergoing gastrectomy for gastric cancer.
- Published
- 2017
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44. Data Sharing: A New Editorial Initiative of the International Committee of Medical Journal Editors. Implications for the Editors´ Network.
- Author
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Alfonso F, Adamyan K, Artigou JY, Aschermann M, Boehm M, Buendia A, Chu PH, Cohen A, Cas LD, Dilic M, Doubell A, Echeverri D, Enç N, Ferreira-González I, Filipiak KJ, Flammer A, Fleck E, Gatzov P, Ginghina C, Goncalves L, Haouala H, Hassanein M, Heusch G, Huber K, Hulín I, Ivanusa M, Krittayaphong R, Lau CP, Marinskis G, Mach F, Moreira LF, Nieminen T, Oukerraj L, Perings S, Pierard L, Potpara T, Reyes-Caorsi W, Rim SJ, Rødevand O, Saade G, Sander M, Shlyakhto E, Timuralp B, Tousoulis D, Ural D, Piek JJ, Varga A, and Lüscher TF
- Subjects
- Humans, Periodicals as Topic, Biomedical Research, Editorial Policies, Information Dissemination, Publishing
- Abstract
The International Committee of Medical Journal Editors (ICMJE) provides recommendations to improve the editorial standards and scientific quality of biomedical journals. These recommendations range from uniform technical requirements to more complex and elusive editorial issues including ethical aspects of the scientific process. Recently, registration of clinical trials, conflicts of interest disclosure, and new criteria for authorship -emphasizing the importance of responsibility and accountability-, have been proposed. Last year, a new editorial initiative to foster sharing of clinical trial data was launched. This review discusses this novel initiative with the aim of increasing awareness among readers, investigators, authors and editors belonging to the Editors´ Network of the European Society of Cardiology.
- Published
- 2017
- Full Text
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45. Bilateral sympathectomy improves postinfarction left ventricular remodeling and function.
- Author
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Zanoni FL, Simas R, da Silva RG, Breithaupt-Faloppa AC, Coutinho E Silva RD, Jatene FB, and Moreira LF
- Subjects
- Animals, Apoptosis, Catecholamines metabolism, Cytokines metabolism, Disease Models, Animal, Fibrosis, Heart Ventricles metabolism, Heart Ventricles pathology, Heart Ventricles physiopathology, Male, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 metabolism, Myocardial Contraction, Myocardial Infarction metabolism, Myocardial Infarction pathology, Myocardial Infarction physiopathology, Natriuretic Peptide, Brain metabolism, Rats, Wistar, Recovery of Function, Stroke Volume, Vascular Endothelial Growth Factor A metabolism, Heart Ventricles innervation, Myocardial Infarction surgery, Sympathectomy methods, Ventricular Function, Left, Ventricular Remodeling
- Abstract
Objectives: To evaluate the influence of bilateral or left sympathectomy on left ventricular remodeling and function after myocardial infarction in rats., Methods: Myocardial infarction was induced in rats by ligation of the left anterior descending coronary. Seven days later, rats were divided into 4 groups: the myocardial infarction, myocardial infarction with left sympathectomy, myocardial infarction with bilateral sympathectomy, and sham groups. After 8 weeks, left ventricular function was evaluated with the use of a pressure-volume conductance catheter under steady-state conditions and pharmacological stress. Infarct size and extracellular matrix fibrosis were evaluated, and cardiac matrix metalloproteinases and myocardial inflammatory markers were analyzed., Results: The myocardial infarction and left sympathectomy group had an increased end diastolic volume, whereas the bilateral sympathectomy group had a mean end-diastolic volume similar to that of the sham group (P < .002). Significant reduction in ejection fraction was observed in the myocardial infarction and left sympathectomy group, whereas it was preserved after bilateral sympathectomy (P < .001). In response to dobutamine, left ventricular contractility increased in sham rats, rising stroke work, cardiac output, systolic volume, end-diastolic volume, ejection fraction, and dP/dt max. Only bilateral sympathectomy rats had significant increases in ejection fraction (P < .001) with dobutamine. Fibrotic tissue and matrix metalloproteinase expression decreased in the bilateral sympathectomy group compared to that in the myocardial infarction group (P < .001) and was associated with left ventricular wall thickness maintenance and better apoptotic markers in noninfarcted myocardium., Conclusions: Bilateral sympathectomy effectively attenuated left ventricular remodeling and preserved systolic function after myocardial infarction induction in rats., (Copyright © 2016 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
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46. Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia.
- Author
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Junqueira Reis LC, Picanço-Castro V, Paes BCMF, Pereira OA, Gerdes Gyuricza I, de Araújo FT, Morato-Marques M, Moreira LF, Costa EBO, Dos Santos TPM, Covas DT, Pereira Carramaschi LDV, and Russo EMS
- Abstract
Sickle cell anemia (SCA) is a monogenic disease of high mortality, affecting millions of people worldwide. There is no broad, effective, and safe definitive treatment for SCA, so the palliative treatments are the most used. The establishment of an in vitro model allows better understanding of how the disease occurs, besides allowing the development of more effective tests and treatments. In this context, iPSC technology is a powerful tool for basic research and disease modeling, and a promise for finding and screening more effective and safe drugs, besides the possibility of use in regenerative medicine. This work obtained a model for study and treatment of SCA using iPSC. Then, episomal vectors were used for reprogramming peripheral blood mononuclear cells to obtain integration-free iPSC. Cells were collected from patients treated with hydroxyurea and without treatment. The iPSCP Bscd lines were characterized for pluripotent and differentiation potential. The iPSC lines were differentiated into HSC, so that we obtained a dynamic and efficient protocol of CD34+CD45+ cells production. We offer a valuable tool for a better understanding of how SCA occurs, in addition to making possible the development of more effective drugs and treatments and providing better understanding of widely used treatments, such as hydroxyurea.
- Published
- 2017
- Full Text
- View/download PDF
47. Impact of Brazilian papers in cardiology and cardiovascular sciences in the last decade.
- Author
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Moreira LF
- Subjects
- Biomedical Research statistics & numerical data, Brazil, Internationality, Periodicals as Topic standards, Time Factors, Cardiology, Journal Impact Factor, Periodicals as Topic statistics & numerical data
- Published
- 2017
- Full Text
- View/download PDF
48. Changes in cellular proliferation and plasma products are associated with liver failure.
- Author
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Melgaço JG, Soriani FM, Sucupira PH, Pinheiro LA, Vieira YR, de Oliveira JM, Lewis-Ximenez LL, Araújo CC, Pacheco-Moreira LF, Menezes GB, Cruz OG, Vitral CL, and Pinto MA
- Abstract
Aim: To study the differences in immune response and cytokine profile between acute liver failure and self-limited acute hepatitis., Methods: Forty-six patients with self-limited acute hepatitis (AH), sixteen patients with acute liver failure (ALF), and twenty-two healthy subjects were involved in this study. The inflammatory and anti-inflammatory products in plasma samples were quantified using commercial enzyme-linked immunoassays and quantitative real-time PCR. The cellular immune responses were measured by proliferation assay using flow cytometry. The groups were divided into viral- and non-viral-induced self-limited AH and ALF. Thus, we worked with five groups: Hepatitis A virus (HAV)-induced self-limited acute hepatitis (HAV-AH), HAV-induced ALF (HAV-ALF), non-viral-induced self-limited acute hepatitis (non-viral AH), non-viral-induced acute liver failure (non-viral ALF), and healthy subjects (HC). Comparisons among HAV and non-viral-induced AH and ALF were performed., Results: The levels of mitochondrial DNA (mtDNA) and the cytokines investigated [interleukin (IL)-6, IL-8, IL-10, interferon gamma, and tumor necrosis factor] were significantly increased in ALF patients, independently of etiology ( P < 0.05). High plasma mtDNA and IL-10 were the best markers associated with ALF [mtDNA: OR = 320.5 (95%CI: 14.42-7123.33), P < 0.0001; and IL-10: OR = 18.8 (95%CI: 1.38-257.94), P = 0.028] and death [mtDNA: OR = 12.1 (95%CI: 2.57-57.07), P = 0.002; and IL-10: OR = 8.01 (95%CI: 1.26-50.97), P = 0.027]. In the cellular proliferation assay, NK
bright , NKT and regulatory T cells (TReg) predominated in virus-specific stimulation in HAV-induced ALF patients with an anergic behavior in the cellular response to mitotic stimulation. Therefore, in non-viral-induced ALF, anergic behavior of activated T cells was not observed after mitotic stimulation, as expected and as described by the literature., Conclusion: mtDNA and IL-10 may be predictors of ALF and death. TReg cells are involved in immunological disturbance in HAV-induced ALF., Competing Interests: Conflict-of-interest statement: The authors declare that they have no competing interests.- Published
- 2016
- Full Text
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49. DO PROXIMAL AND DISTAL GASTRIC TUMOURS BEHAVE DIFFERENTLY?
- Author
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Costa LB, Toneto MG, and Moreira LF
- Subjects
- Adenocarcinoma surgery, Aged, Female, Humans, Lymph Node Excision, Male, Middle Aged, Retrospective Studies, Stomach Neoplasms surgery, Survival Rate, Adenocarcinoma mortality, Adenocarcinoma pathology, Stomach Neoplasms mortality, Stomach Neoplasms pathology
- Abstract
Background: Although the incidence of gastric (adenocarcinoma) cancer has been decreasing over time, it is still one of the most common malignancies worldwide, and proximal tumours tend to have a worse prognosis., Aim: To compare surgical outcomes and prognosis between proximal - excluding tumours of the cardia - and distal gastric cancer., Methods: Out of 293 cases reviewed - 209 with distal and 69 with proximal gastric cancer - were compared for clinical and pathological features, stage, surgical outcome, mortality and survival., Results: Statistically, there was no significant difference between patients in both groups regarding mortality (p=0.661), adjuvant chemotherapy (p 0.661), and radiation (p=1.000). However, there was significant difference in the degree of lymph node dissection employed (p=0.002) and the number of positive lymph nodes resected (p=0.038) between the two groups. The odds of death at five years for patients who had a D0 dissection was three times greater (odds ratio 2.78; (95%CI 1.33-5.82) than that for patients who had a D2 dissection, while for patients who had a D1 dissection the odds ratio was only 1.41 (95%CI 0.71-2.83) compared to D2-dissected patients., Conclusion: Although no significant differences were found between proximal and distal gastric cancer, the increased risk of death in D0- and D1-dissected patients clearly suggests an important role of radical D2 lymph node dissection in survival., Competing Interests: none
- Published
- 2016
- Full Text
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50. Impact of Model for End-stage Liver Disease Score in Post-Liver Transplantation Prognosis.
- Author
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Annunziata TB, Paulino K, Fernandes R, Bento G, Stoduto G, Vasconcelos R, Demetrio L, and Moreira LF
- Subjects
- Adult, Female, Hospitalization, Humans, Male, Middle Aged, Prognosis, Retrospective Studies, Survival Rate, Time Factors, Waiting Lists, Liver Diseases surgery, Liver Transplantation mortality, Models, Statistical, Severity of Illness Index
- Abstract
Although the Model for End-Stage Liver Disease (MELD) score has been used to evaluate mortality of patients on the waiting list for liver transplantation, it has not established if it correlates with post-liver transplantation prognosis. The aim of this study is to assess if there is a relationship between pretransplantation MELD and post-transplantation results, especially regarding 30-day post-LT survival rates. We analyzed data from 172 patients who underwent deceased-donor liver transplantation between February 2013 and May 2015 in a single center. The sample was divided in two groups according to their MELD scores: group 1, with MELD <25 (n = 134), and group 2, with MELD ≥25 (n = 38). The groups were compared with regard to 1-, 3-, and 12-month postoperative survival; total length of stay and in intensive care unit; and perioperative blood transfusion. The global 30-day post-liver transplantation survival rate was 87.2% (89.55% for group 1 and 78.99% for group 2 (P = .090). The evaluation of 3- and 12-month survival showed that in group 2 the mortality was higher (P = .01). Our data suggests a positive relationship between the higher MELD group and longer length of stay in intensive care unit (P = .0345), but not for total time of hospital admission (P = .524). Perioperative blood product transfusion was needed in 35.82% of patients in group 1 and 71.05% in group 2; this correlated with a higher 30- day postoperative mortality (P < .001)., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
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