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ABSTRACT: In the investigation of crimes involving animals and animal-origin products or by-products, forensic veterinary medicine plays a crucial role through examinations and analyses of evidence related to the cases. In addition to veterinary forensics, other forensic sciences frequently contribute to solving these types of crimes. Drawing on the extensive experience of the Federal Police’s criminal investigations in Brazil and the specialized forensic veterinary and scientific expertise developed at the stricto sensu Graduate level at the University of Brasília, this review provides an updated and comprehensive analysis of the most relevant expert examinations supporting investigations into animal-related crimes, with a focus on the Brazilian context. The review covers key aspects of forensic examinations conducted by criminal experts, including forensic entomology, forensic toxicology, imaging techniques, taxonomic identifications, bird rings, investigations of mistreatment, forensic veterinary pathology, forensic genetics, and isotope ratio analysis. The field of veterinary forensics, with its diverse resources, established techniques, highly trained and continually updated experts, and the latest advancements in criminalistics, is essential for properly addressing judicial demands.

Abstract Antimicrobial drug resistance is a challenge to public health. Various microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, Pythium insidiosum, and Candida auris, have developed resistance to commonly used antimicrobials in infection disease treatments. Consequently, there is an urgent need to explore and develop novel therapeutic drugs. Natural products, particularly medicinal plants, have received attention in the quest for innovative treatments for various diseases. Plantago major is a plant endowed with several biological properties, such as antibacterial, antifungal, and healing actions. This study aimed to investigate the antimicrobial activities of the methanolic extract obtained from the leaves of P. major. Microdilution assays were conducted to determine the minimum inhibitory concentration and minimum bactericidal and fungicidal concentration. Additionally, synergism with antimicrobial drugs was assessed using a time-kill curve analysis. A synergistic bactericidal interaction between the extract and imipenem was observed against carbapenemase-producing K. pneumoniae. For MRSA, a bacteriostatic synergism was noted in combinations of the extract with cephalotin and oxacillin. For C. auris, a fungistatic interaction was observed between the extract and amphotericin B. These results suggest the presence of bioactive compounds within the extract with therapeutic potential for combating infections caused by these microorganisms.

This study explored the use of chicken feathers, a low-cost and abundant agricultural byproduct, as a sorbent for the removal of reactive yellow dye from aqueous solutions. The dual potential of feathers as both adsorbents and sorbents, attributed to their keratin-rich structure, was utilized to investigate their effectiveness in dye removal. Feathers, activated with 1.0 mol/L HCl, exhibited a maximum adsorption capacity at 70 °C and pH 5.5, as determined from Langmuir isotherm modeling. A 22 central composite rotatable design revealed that temperature and pH significantly influence the adsorption efficiency, with higher temperatures favoring the process. Kinetic studies demonstrated pseudo-first-order behavior, with rapid initial adsorption reaching equilibrium within 120 min. Thermodynamic analysis confirmed the endothermic nature of the process (ΔH° = 28.04 kJ mol−1), a positive entropy change (ΔS° = 66.62 J/mol·K), and a reduction in Gibbs free energy (ΔG°) with increasing temperature, suggesting enhanced feasibility at elevated temperatures. This research highlights the potential of utilizing poultry industry residues as sustainable and efficient sorbents for environmental remediation, contributing to waste valorization and eco-friendly wastewater treatment solutions.

The present systematic review (SR) aimed to analyze the impact of cigarette taxes and prices on the prevalence of tobacco smoking in Latin America countries. The methods of this SR were previously established and registered in PROSPERO (CRD42022319407) and was reported according to the PRISMA checklist. The searches were made in 5 databases (PubMed/MEDLINE, Scopus, Embase, Web of Science and LILACS) in addition to the grey literature, without limitation of year, and published in English, Spanish or Portuguese. Studies which analyzed cigarette taxes and prices on tobacco smoking prevalence in Latin America were eligible for inclusion. The study selection was conducted in two phases by two independently reviewers. Seven observational studies conducted in Brazil, Mexico and Colombia were included in the qualitative analysis. The risk of bias (RoB) of each study was assessed using the Joanna Briggs Institute checklist. All studies found that an increase in excise taxes of cigarette led to an increase in the retail price. Four studies (57.1 %) reported that increasing cigarette taxes and prices was effective to decrease the prevalence of tobacco smoking. The included studies were graded as having a low (57.1 %) or moderate (42.8 %) RoB. The use of cigarette taxes and prices measures are effective tools to reduce the prevalence of tobacco smoking. However, it is vital the development of further research on this topic in other countries of Latin America since the included studies were performed only in Brazil, Colombia and Mexico.

ABSTRACT The hydrogen sulfide (H2S) present in biogas needs to be removed due to concerns about corrosion during transportation, storage, health and safety. One of the existing removal processes is biological, using a biotrickling filter (BTF). In this study, the performance of full-scale BTF for H2S removal under different operating conditions was evaluated. The BTF system was operated for 300 days, during which two spraying regimes (constant and intermittent) and two sources of nitrate (NO3-) as nutrient solution were evaluated (residual effluent from pig farming and synthetic solution prepared with commercial NaNO3). The performance was monitored by the following parameters: removal efficiency (RE), elimination capacity (EC), pH, dissolved oxygen (DO), empty bed residence time (EBRT) and nitrate concentration (NO3-). The results showed an REH2S = 36.3% with an EC= 1.95 gH2S m-3 d-1 for constant spraying, RE= 99.59% and EC= 4.2 gH2S m-3 d-1 for intermittent spraying with residual effluent from pig farming and RE=99.26% and EC= 4.13 gH2S m-3 d-1 with synthetic solution prepared with commercial NaNO3 solution. The results indicate that intermittent spraying provides better efficiency in the removal of H2S from biogas regardless of the nitrate source (effluent or synthetic medium).

Space syntax is utilized to model flows through open and closed built environments, which enables project innovation by rethinking the design of spaces on a global scale with better flow quality. Therefore, studies focused on spatial syntax, when related to comprehensive flows in open and closed spaces, provide a holistic and valuable understanding of the dynamics of user flows in the urban environment from a perspective centered on the United Nation’s Sustainable Development Goal (SDG) 11. This goal requires urban planners and designers to use approaches that support future decisions focused on urban design and planning. The object of investigation of this bibliographic study consists of an approach to representing space syntax in open spaces and closed spaces on a global scale. This study aims to analyze the concepts of pedestrian flows (open and closed) by a space syntax-based bibliographical approach on a global scale, demonstrating the capability of improvements in SDG 11 as applied to the architecture of sustainable flows. Methodologically, bibliographic searches were carried out using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method in databases linked to Scopus and ScienceDirect, focusing on space syntax in relation to the following terms: “open spaces” and “closed spaces”. Using the PRISMA method, 1986 manuscripts dealing with the term “open spaces” and 454 manuscripts dealing with the term “closed spaces” were identified, with the manuscripts most relevant to the topic being selected, followed by a frequency analysis based on a Content Analysis Method (CAM) to identify words with a degree of similarity, related to “spatial syntax: flow in urban environments” and “spatial syntax in closed built systems” in relation to the SDG 11. The results demonstrate the relevance of seven manuscripts in open spaces and four manuscripts in closed spaces related to space syntax. Frequency analysis identified open spaces and produced terms with frequencies such as space = 79 and shape = 46, showing a higher frequency in flows. In closed spaces, the literature has shown that the central term corresponds to space = 79 and flow = 76, making it possible to evaluate flows in circulation areas within the built environment. This study allows a better understanding of flows, highlighting the importance of the urban architecture in the functionality of user flows in sustainable environments, which is capable of contributing to the SDG 11, in the interface of architectural projects on a global scale.

The objective of the current investigation was to evaluate several Eimeria challenges and the resulting alterations in intestinal permeability, intestinal morphology, and intestinal lesion scores in broiler chickens. This study included four groups with ten replicate cages per treatment, in which each group received a different treatment dosage of Eimeria, characterizing high, medium-high, and medium-low challenges. Five days after the challenge, intestinal lesions and permeability were assessed. The results showed that the increase in Eimeria challenge led to a considerable decrease in the height of intestinal villosities, in the ratio between villosity size and crypt depth, and in goblet cells. Moreover, after the challenge, there was a considerable increase in intestinal permeability. In conclusion, the medium-low, medium-high, and high-challenge models can be utilized for experimental infection. In the context of clinical studies, it has been observed that the administration of medium-high and high-challenge doses has proven to be adequate. However, it is advisable to utilize a medium-low challenge level to develop a subclinical challenge model for forthcoming investigations that aim to evaluate nutritional recommendations.

ObjectiveChikungunya virus (CHIKV) causes persistent arthritis, and our prior study showed that approximately one third of CHIKV arthritis patients had exacerbated arthritis associated with exercise. The underlying mechanism of exercise-associated chikungunya arthritis flare (EACAF) is unknown, and this analysis aimed to examine the regulatory T-cell immune response related to CHIKV arthritis flares.MethodsIn our study, 124 Colombian patients with a history of CHIKV infection four years prior were enrolled and 113 cases with serologically confirmed CHIKV IgG were used in this analysis. Patient information was gathered via questionnaires, and blood samples were taken to identify total live peripheral blood mononuclear cells, CD4+ cells, T regulatory cells, and their immune markers. We compared outcomes in CHIKV patients with (n = 38) vs. without (n = 75) EACAF using t-tests to assess means and the Fisher's exact test, chi-squared to evaluate categorical variables, and Kruskal-Wallis tests in the setting of skewed distributions (SAS 9.3).Results33.6% of CHIKV cases reported worsening arthritis with exercise. EACAF patients reported higher global assessments of arthritis disease ranging from 0-100 (71.2 ± 19.7 vs. 59.9 ± 28.0, p=0.03). EACAF patients had lower ratios of T regulatory (Treg)/CD4+ T-cells (1.95 ± 0.73 vs. 2.4 ± 1.29, p = 0.04) and lower percentage of GARP (glycoprotein-A repetitions predominant) expression per Treg (0.13 ± 0.0.33 vs. 0.16 ± 0.24 p= 0.020).ConclusionThese findings suggest relative decreases in GARP expression may indicate a decreased level of immune suppression. Treg populations in patients with CHIKV arthritis may contribute to arthritis flares during exercise, though current research is conflicting.

Escherichia coli is a major player in foodborne illnesses, capable of forming biofilms on dairy facilities, leading to milk contamination. Thus, we examined the capacity of E. coli strains from raw milk bulk tanks to form biofilms on surfaces made of polystyrene, stainless steel, and silicone; the potential links between biofilm formation with genes responsible for fimbriae and virulence factors of extra-intestinal E. coli (ExPEC); and the susceptibility of biofilm-forming isolates to iodine and chlorhexidine digluconate. Out of 149 E. coli strains, 42.28% (63/149) formed biofilm on polystyrene, 56.38% (84/149) on silicone, and 21.48% (32/149) on stainless steel. The frequency of genes was: fimH (100%), hlyA (5.4%), irp2 (2.7%), sitA (10.7%), ompT (43.6%), and traT (98%). No biofilm developed when disinfectants were used prior to biofilm formation. However, iodine and chlorhexidine digluconate allowed 25.40% (16/63) of isolates displaying growth after a mature biofilm was formed. The presence of biofilm on different surfaces emphasizes the vital need for thorough equipment cleaning, both in farms and in industrial dairy settings. Rapid disinfection is crucial, given the reduced susceptibility of potentially pathogenic E. coli after biofilm maturity.

Sweeteners are food additives consumed worldwide, especially by individuals with restrictive diets. This study evaluated the cytotoxic and genotoxic effects of sweeteners widely sold in Brazil through a bioassay with Allium cepa. Onion bulbs were exposed to different concentrations of sweeteners, and their root cells were stained with acetic carmine and analyzed under an optical microscope. Distilled water was used as a negative control, and glyphosate as a positive control. The results demonstrated that the mitotic index (MI) and the chromosome aberration index (CAI) of the three sweeteners tested did not differ significantly from the negative control, indicating that they are not toxic or mutagenic additives. This result is positive for consumers of these products, especially those who need to replace sugar due to dietary restrictions. However, they should be consumed with caution.

Staphylococcus aureus stands out as one of the most virulent pathogens in the genus Staphylococcus. This characteristic is due to its ability to produce a wide variety of staphylococcal enterotoxins (SEs) and exotoxins, which in turn can cause staphylococcal food poisoning (SFP), clinical syndromes such as skin infections, inflammation, pneumonia, and sepsis, in addition to being associated with the development of inflammation in the mammary glands of dairy cattle, which results in chronic mastitis and cell necrosis. SEs are small globular proteins that combine superantigenic and emetic activities; they are resistant to heat, low temperatures, and proteolytic enzymes and are tolerant to a wide pH range. More than 24 SE genes have been well described (SEA-SEE, SEG, SEH, SEI, SEJ, SElK, SElL, SElM, SElN, SElO, SElP, SElQ, SElR, SElS, SElT, SElU, SElV, SElW, SElX, SElY, and SElZ), being a part of different SFP outbreaks, clinical cases, and isolated animal strains. In recent years, new genes (sel26, sel27, sel28, sel31, sel32, and sel33) from SEs have been described, as well as two variants (seh-2p and ses-3p) resulting in a total of thirty-three genes from Ses, including the nine variants that are still in the process of genetic and molecular structure evaluation. SEs are encoded by genes that are located in mobile genetic elements, such as plasmids, prophages, pathogenicity islands, and the enterotoxin gene cluster (egc), and housed in the genomic island of S. aureus. Both classical SEs and SE-like toxins (SEls) share phylogenetic relationships, structure, function, and sequence homology, which are characteristics for the production of new SEs through recombination processes. Due to the epidemiological importance of SEs, their rapid assessment and detection have been crucial for food security and public health; for this reason, different methods of identification of SEs have been developed, such as liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS), molecular methods, and whole-genome sequencing; providing the diagnosis of SEs and a better understanding of the occurrence, spread, and eradication of SEs. This review provides scientific information on the enterotoxins produced by S. aureus, such as structural characteristics, genetic organization, regulatory mechanisms, superantigen activity, mechanisms of action used by SEs at the time of interaction with the immune system, methods of detection of SEs, and recent biocontrol techniques used in food.

Introduction: Current recommendations for cardiovascular disease (CVD) prevention are guided CVD risk classification. This study aims to analyze the level of compliance with pharmacological and non-pharmacological recommendations outlined in the World Health Organization (WHO) CVD prevention clinical practice guideline within the general population of two cities in Argentina. Methods: We analyzed the compliance with preventive recommendations from the WHO 2007 Guidelines for Management of Cardiovascular Risk in two cities of Argentina participating in the CESCAS population-based study in the Southern Cone of Latin America. Results: 3990 participants were included. Considering the WHO recommendations, the use of antiplatelet medication was 5.6% (95% CI 3.5, 8.9) in primary prevention and 20.5% (95%= CI 16.0, 25.9) in secondary prevention. Regarding lipid-lowering medication, it was 6.7% (CI 95%= 4.4, 10.1) and 15.4% (CI 95%= 11.6, 20.1), respectively. As per non-pharmacological recommendations in the general population: low intake of fruit and vegetables was 78.4% (CI 95%= 76.8, 79.9); low physical activity was 26.9% (CI 95%= 25.3, 28.5), current cigarette smoking was 28.3% (CI 95%= 26.6, 30.0), overweight/obesity was 73.9% (CI 95%= 72.3, 75.6), and excessive alcohol intake was 2.6% (CI 95%= 2.1, 3.4). Conclusions: It was observed a significantly low compliance with pharmacological and non-pharmacological recommendations for CVD prevention in the general population of two cities in Argentina. Urgent efforts are needed to improve compliance to cardiovascular preventive recommendations promoted by CPG, especially in Low- and Middle- Income Countries.

BackgroundRecently published studies have reported association of COVID-19 vaccine ChAdOx1-S (Vaxzevria) with Guillain Barré Syndrome (GBS). Less is known about the safety of other COVID-19 vaccines with respect to GBS outcome. This study investigated the association of COVID-19 vaccines with GBS in more than 15 million persons aged ≥12 years in Italy.MethodsStudy population was all individuals aged ≥12 years who received at least one dose of COVID-19 vaccines, admitted to emergency care/hospital for GBS from 27 December 2020-30 September 2021 in Italy. Identification of GBS cases and receipt of at least one dose of mRNA-1273 (Elasomeran), BNT162b2 (Tozinameran), ChAdOx1-S (Vaxzevria) and Ad26.COV2.S (Janssen) through record linkage between regional health care and vaccination registries. Relative Incidence (RI) was estimated Self-controlled case series method adapted to event-dependent exposure using in the 42-day exposure risk period after each dose compared with other observation periods.ResultsIncreased risk of GBS was found after first (RI = 6.83; 95% CI 2.14-21.85) and second dose (RI = 7.41; 2.35-23.38) of mRNA-1273 and first dose of ChAdOx1-S (RI = 6.52; 2.88-14.77). Analysis by age found an increased risk among those aged≥60 years after first (RI = 8.03; 2.08-31.03) and second dose (RI = 7.71; 2.38-24.97) of mRNA-1273. The first dose of ChAdOx1-S was associated with GBS in those aged 40-59 (RI = 4.50; 1.37-14.79) and in those aged ≥ 60 years (RI = 6.84; 2.56-18.28).ConclusionsmRNA-1273 and ChAdOx1-S vaccines were associated with an increased risk of GBS however this risk resulted in a small number of excess cases. Limitations were loss of GBS outpatient cases and imprecision of the estimates in the subgroup analysis due to a low number of events.