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5. Fabrication of a three-dimensional scaffold-free trachea with horseshoe-shaped hyaline cartilage.

Author

Uchida, Fumitake, Matsumoto, Keitaro, Nishimuta, Masato, Matsumoto, Takamune, Oishi, Kaido, Hara, Ryosuke, Machino, Ryusuke, Taniguchi, Daisuke, Oyama, Shosaburo, Moriyama, Masaaki, Tomoshige, Koichi, Doi, Ryoichiro, Obata, Tomohiro, Miyazaki, Takuro, Nonaka, Takashi, Nakayama, Koichi, and Nagayasu, Takeshi

Subjects
CARTILAGE regeneration, GROWTH differentiation factors, STEM cell culture, STEM cell factor, TRACHEAL cartilage
Abstract

OBJECTIVES Tracheal regeneration is challenging owing to its unique anatomy and low blood supply. Most tracheal regeneration applications require scaffolds. Herein, we developed bio-three-dimensional-printed scaffold-free artificial tracheas. METHODS We fabricated bio-three-dimensional-printed artificial tracheas. Their anterior surface comprised hyaline cartilage differentiated from mesenchymal stem cells, and their posterior surface comprised smooth muscle. Human bone marrow-derived mesenchymal stem cells were cultured and differentiated into chondrocytes using fibroblast growth factor-2 and transforming growth factor-beta-3. Initially, horseshoe-shaped spheroids were printed to cover the anterior surface of the artificial trachea, followed by the application of human bronchial smooth muscle cells for the posterior surface. After a 3-week maturing process, the artificial trachea was subjected to histological and immunohistochemical analyses. RESULTS The anterior surface of the artificial trachea comprised well-differentiated hyaline cartilage from human bone marrow-derived mesenchymal stem cells. Immunohistochemistry revealed that the smooth muscle expressed α-smooth muscle actin and smooth muscle myosin heavy chain 11. CONCLUSIONS A bio-three-dimensional-printed scaffold-free artificial trachea comprising different tissues at the front and back was successfully fabricated. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Utility of Transanal Tube for Preventing Severe Anastomotic Leakage Following Low Anterior Resection for Patients With Diverting Stoma

Author

Tominaga, Tetsuro, primary, Nonaka, Takashi, additional, Fukuda, Akiko, additional, Moriyama, Masaaki, additional, Oyama, Shosaburo, additional, Ishii, Mitsuhisa, additional, Sumida, Yorihisa, additional, Takeshita, Hiroaki, additional, Hisanaga, Makoto, additional, Fukuoka, Hidetoshi, additional, Tou, Kazuo, additional, Tanaka, Kenji, additional, Sawai, Terumitsu, additional, and Nagayasu, Takeshi, additional

Published
2024
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12. Intraneural fibrosis within ilioinguinal nerve in inguinal hernia patients with preoperative pain -It’s the sign of irreversible nerve injury, isn’t it?

Author

Narita, Masato, primary, Moriyoshi, Koki, additional, Yamaoka, Ryoya, additional, Moriyama, Masaaki, additional, Degawa, Kanako, additional, Fushitani, Masashi, additional, Kojima, Hiroya, additional, Suenaga, Takahiro, additional, Hiroki, Nakanishi, additional, Nishikawa, Gen, additional, Nakanishi, Yasutaka, additional, and Hata, Hiroaki, additional

Published
2023
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15. Robotic anal preserving posterior pelvic exenteration combined with the transanal-vaginal approach

Author

Yamazaki, Shoto, Tominaga, Tetsuro, Nonaka, Takashi, Moriyama, Masaaki, Oyama, Shosaburo, Ishii, Mitsuhisa, Sawai, Terumitsu, and Nagayasu, Takeshi

Subjects
posterior pelvic exenteration, robotic surgery, transanal approach
Abstract

Robotic surgery is increasingly being applied for rectal cancer and its feasibility and safety have been reported. However, problems associated with advanced robotic surgery such as pelvic exenteration include lengthy operation time and difficulty in controlling unexpected bleeding. A 47-year-old woman had undergone laparoscopic left hemicolectomy for descending colon cancer three years previously (pT3N0M0 pStageII). And had undergone bilateral oophorectomy for ovarian metastases one year previously. Follow-up CT detected a peritoneal metastasis in the pelvic space. After seven courses of systemic chemotherapy, she received robotic anal preserving posterior pelvic exenteration combined with the transanal-vaginal approach. The postoperative course was uneventful. There is no evidence of recurrent disease 8 months after surgery. In conclusion, robotic anal preserving posterior pelvic exenteration combined with the transanal-vaginal approach is a safe and feasible minimally invasive approach for the treatment of advanced rectal malignancies., Acta medica Nagasakiensia, 66(1), pp.35-39; 2022

Published
2022

17. Fabrication of a Bio-3d Printed Scaffold-Free Artificial Trachea with Horseshoe-Shaped Hyaline Cartilage Differentiated from Mesenchymal Stem Cells and Smooth Muscles

Author

Uchida, Fumitake, primary, Matsumoto, Keitaro, additional, Nishimuta, Masato, additional, Matsumoto, Takamune, additional, Oishi, Kaido, additional, Hara, Ryosuke, additional, Machino, Ryusuke, additional, Taniguchi, Daisuke, additional, Oyama, Shosaburo, additional, Moriyama, Masaaki, additional, Koichi, Tomoshige, additional, Doi, Ryoichiro, additional, Obata, Tomohiro, additional, Miyazaki, Takuro, additional, Nonaka, Takashi, additional, Nakayama, Koichi, additional, and Nagayasu, Takeshi, additional

Published
2023
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19. 323. CLINICAL CHARACTERISTICS OF HIATAL HERNIA WITH ANEMIA AND SIGNIFICANCE OF SURGICAL REPAIR

Author

Nakanishi, Yasutaka, primary, Hata, Hiroaki, additional, Suenaga, Takahiro, additional, Moriyama, Masaaki, additional, Degawa, Kanako, additional, Kojima, Hiroya, additional, Kanbe, Hiroyuki, additional, Miki, Akimori, additional, Nakanishi, Hiroki, additional, Yamaoka, Ryoya, additional, Matsusue, Ryo, additional, Narita, Masato, additional, Otani, Tetsushi, additional, and Yamaguchi, Takashi, additional

Published
2022
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21. Successful use of bio plugs for delayed bronchial closure after pneumonectomy in experimental settings

Author

Moriyama, Masaaki, Matsumoto, Keitaro, Taniguchi, Daisuke, Machino, Ryusuke, Tsuchiya, Tomoshi, Nakayama, Koichi, Nagayasu, Takeshi, Moriyama, Masaaki, Matsumoto, Keitaro, Taniguchi, Daisuke, Machino, Ryusuke, Tsuchiya, Tomoshi, Nakayama, Koichi, and Nagayasu, Takeshi

Abstract

OBJECTIVES: Cell therapies, such as stem cell suspension injection, are used to treat bronchopleural fistula. Although it is safe and effective, injected cells cannot remain within the bronchioles of the fistula due to cell leakage into the thoracic cavity. Here, we inserted a ‘bio plug’ into the fistula, produced using cells and a bio-3D printer, to examine the effectiveness of bio plugs for the closure of bronchopleural fistulas, the optimal cell source and the closure mechanism. METHODS: Bio plugs were made with mesenchymal stem (stromal) cells derived from bone marrow (MSCBM), fibroblasts and rat lung micro-vessel endothelial cells using a bio-3D printer with different cell mixing ratios. Six groups, according to the presence or absence and the type of bio plugs, were compared. The plugs were inserted into the bronchi of F344 rats. The obstruction ratio and histological and immunohistochemical findings were evaluated. RESULTS: MSCBM+ rat lung micro-vessel endothelial cell group exhibited a higher obstruction ratio among all groups excluding the MSCBM group (P = 0.039). This group had fibrosis and CD31-positive cells and fewer CD68-positive cells than MSCBM and MSCBM+ fibroblast groups. CONCLUSIONS: Bio plugs with mixed cells, including stem cells, contribute to bronchial closure in the current experimental setting. Endothelial cells effectively maintain the structure in this model. Although bronchial closure for bronchopleural fistula could not be described as clinical conditions were not reproduced, we collected essential data on bronchial closure; however, further experiments are warranted., Interactive CardioVascular and Thoracic Surgery, 34(4), pp660-667; 2022

Published
2022

22. Efficacy of risk-stratified indicators for adjuvant chemotherapy with fluorouracil and oxaliplatin after hepatectomy for colorectal cancer liver metastasis Running title: Risk-stratified indication for doublet AC after hepatectomy for CRLM

Author

Tominaga, Tetsuro, primary, Maruyama, Keizaburo, additional, Nonaka, Takashi, additional, Moriyama, Masaaki, additional, Oyama, Shosaburo, additional, Ishii, Mitsutoshi, additional, Sawai, Terumitsu, additional, and Nagayasu, Takeshi, additional

Published
2022
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23. Mirtazapine suppresses dopamine neurodegeneration by inducing metallothionein expression via stimulation on serotonin 1A receptor of astrocyte.

Author

Ryo, Kikuoka, primary, Miyazaki, Ikuko, additional, Kubota, Natsuki, additional, Maeda, Megumi, additional, Kagawa, Daiki, additional, Moriyama, Masaaki, additional, Sato, Asuka, additional, Murakami, Shinki, additional, Kitamura, Yoshihisa, additional, Sendo, Toshiaki, additional, Zamami, Yoshito, additional, and Asanuma, Masato, additional

Published
2022
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24. Clinical Complete Response Using Pembrolizumab in a Patient with Retroperitoneal Recurrence after Resection for Ascending Colon Cancer withSynchronous Brain Metastasis

Author

Ono, Rika, primary, Tominaga, Tetsuro, additional, Nonaka, Takashi, additional, Moriyama, Masaaki, additional, Oyama, Shosaburo, additional, Ishii, Mitsutoshi, additional, Hamasaki, Keiko, additional, Arai, Jyunichi, additional, Sawai, Terumitsu, additional, and Nagayasu, Takeshi, additional

Published
2022
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25. A Case of Extra-Adrenal Myelolipoma Arising from the Right Lateral Pelvic Area

Author

Tominaga, Tetsuro, primary, Nonaka, Takashi, additional, Fukuda, Akiko, additional, Moriyama, Masaaki, additional, Oyama, Shosaburo, additional, Hashimoto, Yasumasa, additional, Hamasaki, Keiko, additional, Hidaka, Shigekazu, additional, Shigematsu, Kazuhito, additional, Abe, Kuniko, additional, Sawai, Terumitsu, additional, and Nagayasu, Takeshi, additional

Published
2021
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29. Impact of obesity on short‐term outcomes of laparoscopic colorectal surgery for Japanese patients with colorectal cancer: A multicenter study

Author

Yamashita, Mariko, primary, Tominaga, Tetsuro, additional, Nonaka, Takashi, additional, Fukda, Akiko, additional, Moriyama, Masaaki, additional, Oyama, Syosaburo, additional, Tanaka, Kenji, additional, Hamada, Kiyoaki, additional, Araki, Masato, additional, Sumida, Yorihisa, additional, Takeshita, Hiroaki, additional, Hisanaga, Makoto, additional, Fukuoka, Hidetoshi, additional, Wada, Hideo, additional, Tou, Kazuo, additional, Sawai, Terumitsu, additional, and Nagayasu, Takeshi, additional

Published
2020
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30. A new method using a vessel-sealing system provides coagulation effects to various types of bleeding with less thermal damage

Author

Oyama, Shosaburo, primary, Nonaka, Takashi, additional, Matsumoto, Keitaro, additional, Taniguchi, Daisuke, additional, Hashimoto, Yasumasa, additional, Obata, Tomohiro, additional, Hisanaga, Makoto, additional, Moriyama, Masaaki, additional, Matsuo, Naoto, additional, Wada, Hideo, additional, Hamada, Kiyoaki, additional, Wakata, Kouki, additional, Tominaga, Tetsuro, additional, Hidaka, Shigekazu, additional, Sawai, Terumitsu, additional, and Nagayasu, Takeshi, additional

Published
2020
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32. Usefulness of structured‐cadaveric training for trans‐anal pelvic exenteration.

Author

Tominaga, Tetsuro, Nonaka, Takashi, Fukuda, Akiko, Moriyama, Masaaki, Oyama, Shosaburo, Ishii, Mitsutoshi, Takamura, Keiko, Tsurumoto, Toshiyuki, Sawai, Terumitsu, and Nagayasu, Takeshi

Subjects
PELVIC exenteration, SURGEONS
Abstract

Purpose Structured training using cadaveric simulation is useful for trans‐anal surgery; however, no studies have examined the effectiveness of cadaveric training for advanced trans‐anal surgery including pelvic exenteration (PE). Methods: Twelve colorectal surgeons attended a total of 10 cadaveric simulation training courses between 2016 and 2021 and completed a questionnaire at the end of the program. We divided 14 consecutive patients who underwent trans‐anal PE between 2015 and 2021 into two groups: pre‐training group and post‐training group, and compared the clinico‐pathological features between the groups. Results: The median length of clinical experience of the surgeons was 12 years. There was high score agreement among the surgeons that the course was useful for recognition of anatomical and layer structure, training for trans‐anal total mesorectal excision and trans‐anal PE, and reducing complications specific to the trans‐anal approach. Compared with the pre‐training group, patients in the post‐training group had a higher rate of two‐team surgery (77.8% vs 0%, P =.021), and shorter time to specimen removal (273 vs 423 min, P =.045). Conclusions: Structured‐cadaveric training has potential use as a technical step‐up in advanced trans‐anal surgery that might contribute to better short‐term outcomes in the clinical setting. [ABSTRACT FROM AUTHOR]

Published
2022
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33. Abstracts of selected papers presented at the 78th general meeting of the Japanese Society of Gastroenterology

Author

Kusano, Motoyasu, Sekiguchi, Toshikazu, Hanyu, Nobuyoshi, Aoki, Teruaki, Matsushima, Yasuhiro, Okamoto, Eizo, Takeda, Yasuo, Takeda, Ryoyu, Miyachi, Masahiko, Nimura, Yuji, Arai, Taidoh, Masuda, Jun, Tanaka, Masao, Ogawa, Yoshiaki, Ura, Kazuhide, Matsumoto, Teiji, Taniyama, K., Kurosawa, Susumu, Owyang, Chung, Baba, Hiroshi, Fujimura, Masaki, Hamada, Eiji, Shimada, Tadahito, Kabemura, Teppei, Chijiiwa, Yoshiharu, Sato, T., Koito, K., Matsuda, Hiroko, Kawasaki, Tsunehisa, Obara, Katsutoshi, Kasukawa, Reiji, Miyoshi, Hirofumi, Matsumoto, Akio, Yamamoto, Manabu, Ohmasa, Ryouji, Kokubu, Shigehiro, Shibata, Hisao, Tajiri, Takashi, Onda, Masahiko, Hashizume, Makoto, Sugimachi, Keizo, Kobayashi, Kenji, Shiozaki, Hitoshi, Fujisaki, Junko, Shimoda, Tadakazu, Hase, Satoshi, Tsukamoto, Yoshihisa, Atsumi, M., Konishi, H., Nakajo, Shinobu, Fujiyama, Yoshihide, Taruishi, Masaki, Ayabe, Tokiyoshi, Morise, Kimitomo, Yamaguchi, Takeo, Makiyama, Kazuya, Itsuno, Minoru, Matsui, T., Okabe, N., Okabe, Nobuo, Matsui, Toshiyuki, Sakatani, Arata, Koizumi, Koichi, Imai, Yutaka, Sugino, Yoshinori, Masaki, Tadahiko, Sawada, Toshio, Nishigami, Takashi, Satomi, Masamichi, Hatada, Yasumasa, Saito, Hiroshi, Kobayshi, Kiyonori, Katsumata, Tomoe, Sugimoto, Kenji, Itabashi, Tsukasa, Kitagawa, Motoji, Hayakawa, Tetsuo, Okazaki, Kazuichi, Yamamoto, Yasuro, Funakoshi, Akihiro, Miyasaka, Kyoko, Soejima, Kazuhiko, Kanda, Mikio, Sugiyama, Masanori, Kuroda, Akira, Inoue, Hisayuki, Bamba, Tadao, Hamanaka, Y., Suzuki, T., Suzuki, Mamoru, Hanyu, Fujio, Tamura, Katsuhiro, Nakase, Akira, Noguchi, M., Hiwatashi, N., Murata, Yuhji, Suzuki, Kazuo, Ohtani, Haruo, Watanabe, Yoshihisa, Zeniya, Mikio, Aizawa, Yoshio, Masumoto, T., Onji, M., Hamasaki, Keisuke, Nakata, Keisuke, Fukui, Hiroshi, Tsujii, Tadasu, Yamashiki, Masayoshi, Nishimura, Akira, Tsutsui, Hiroko, Mizoguchi, Yasuhiro, Kimura, Fumio, Miyazaki, Masaru, Kiyota, Keisuke, Inokuchi, Hideto, Yamamoto, Yoshihiro, Oka, Hiroshi, Horikoshi, Tsutomu, Sekiguchi, Toshikazu, Takayasu, H., Shirai, T., Hongo, Michio, Okuno, Yo, Okano, H., Aoyama, N., Ohsuki, Masao, Maeda, Kenji, Haruma, Ken, Sumii, Koji, Nakai, Yoshihide, Fukunaga, Mikihiko, Kaneko, Hiroshi, Morise, Kimitomo, Okumura, Toshikatsu, Uehara, Akira, Fukuda, Yoshihiro, Satomi, Masamichi, Joh, Takashi, Itoh, Makoto, Kitagawa, Yuko, Kitajima, Masaki, Iwao, Tadashi, Toyonaga, Atsushi, Nakamura, Masahiko, Oda, Masaya, Kawamura, Yukimitsu, Dohden, Kenji, Kamiyama, Yasuhiko, Matsuno, Seiki, Hirano, Morihisa, Otsuka, Sachio, Ito, Masahiro, Sekine, Ichiro, Ogihara, Tatsuo, Sato, Nobuhiro, Uehara, Akira, Namiki, Masayoshi, Sugiura, Nobuyuki, Ebara, Masaaki, Matsuo, Naoki, Uchida, Hideo, Okada, Shuichi, Okazaki, Nobuo, Tsujii, Hirohiko, Ohsuga, Toshiaki, Abe, M., Nagata, Y., Yamasaki, Susumu, Kosuge, Tomoo, Kitamoto, Mikiya, Nakanishi, Toshio, Ichikawa, Yuzo, Mizoguchi, Yasuhiro, Ohtake, Yoshio, Hirasawa, Hiroyuki, Sugihara, Junichi, Muto, Yasutoshi, Yasunaga, Mitsuru, Okita, Kiwamu, Ukida, Minoru, Tsuji, Takao, Isai, Hideya, Uchino, Junichi, Suzuki, Katsuhiko, Komatsu, Kanji, Ohtomo, Yumiko, Idezuki, Yasuo, Sakuramachi, Shunji, Kimura, Taizo, Kitano, Seigo, Sugimachi, Keizo, Moriyama, Masaaki, Yazaki, Yasuyuki, and Saito, Takashige

Published
1993
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37. Correction: Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing

Author

Takeoka, Yosuke, primary, Matsumoto, Keitaro, additional, Taniguchi, Daisuke, additional, Tsuchiya, Tomoshi, additional, Machino, Ryusuke, additional, Moriyama, Masaaki, additional, Oyama, Shosaburo, additional, Tetsuo, Tomoyuki, additional, Taura, Yasuaki, additional, Takagi, Katsunori, additional, Yoshida, Takuya, additional, Elgalad, Abdelmotagaly, additional, Matsuo, Naoto, additional, Kunizaki, Masaki, additional, Tobinaga, Shuichi, additional, Nonaka, Takashi, additional, Hidaka, Shigekazu, additional, Yamasaki, Naoya, additional, Nakayama, Koichi, additional, and Nagayasu, Takeshi, additional

Published
2019
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38. Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing

Author

Takeoka, Yosuke, primary, Matsumoto, Keitaro, additional, Taniguchi, Daisuke, additional, Tsuchiya, Tomoshi, additional, Machino, Ryusuke, additional, Moriyama, Masaaki, additional, Oyama, Shosaburo, additional, Tetsuo, Tomoyuki, additional, Taura, Yasuaki, additional, Takagi, Katsunori, additional, Yoshida, Takuya, additional, Elgalad, Abdelmotagaly, additional, Matsuo, Naoto, additional, Kunizaki, Masaki, additional, Tobinaga, Shuichi, additional, Nonaka, Takashi, additional, Hidaka, Shigekazu, additional, Yamasaki, Naoya, additional, Nakayama, Koichi, additional, and Nagayasu, Takeshi, additional

Published
2019
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39. Impact of obesity on short‐term outcomes of laparoscopic colorectal surgery for Japanese patients with colorectal cancer: A multicenter study.

Author

Yamashita, Mariko, Tominaga, Tetsuro, Nonaka, Takashi, Fukda, Akiko, Moriyama, Masaaki, Oyama, Syosaburo, Tanaka, Kenji, Hamada, Kiyoaki, Araki, Masato, Sumida, Yorihisa, Takeshita, Hiroaki, Hisanaga, Makoto, Fukuoka, Hidetoshi, Wada, Hideo, Tou, Kazuo, Sawai, Terumitsu, and Nagayasu, Takeshi

Subjects
COLORECTAL cancer, ONCOLOGIC surgery, JAPANESE people, OBESITY, LAPAROSCOPIC surgery, PROCTOLOGY
Abstract

Introduction: The impact of obesity on short‐term outcomes after laparoscopic colorectal surgery (LAC) in Asian patients is unclear. The purpose of the present multicenter study was to evaluate the safety and feasibility of LAC in obese Japanese patients. Methods: We retrospectively reviewed 1705 patients who underwent LAC between April 2016 and February 2019. Patients were classified according to body mass index (BMI): non‐obese (BMI < 25 kg/m2, n = 1335), obese I (BMI 25‐29.9 kg/m2, n = 313), and obese II (BMI ≥30 kg/m2, n = 57). Clinical characteristics and surgical outcomes were compared among the three groups. Results: The proportion of patients with comorbidities (non‐obese, 58.1%; obese I, 69.6%; obese II, 75.4%; P <.001) and median operation time (non‐obese, 224 minutes; obese I, 235 minutes; obese II, 258 minutes; P =.004) increased significantly as BMI increased. The conversion rate was similar among the groups (P =.715). Infectious complications were significantly high in obese II patients (non‐obese, 10.4%; obese I, 8.3%; obese II, 28.1%; P <.001). Multivariate analysis revealed that in obese II patients, BMI was an independent predictive factor of infectious postoperative complications (odds ratio 2.648; 95% confidence interval, 1.421‐4.934; P =.002). Conclusion: LAC has an increased risk of postoperative infectious complications in obese II patients, despite improvements in surgical technique. Management of obese II colorectal cancer patients requires meticulous perioperative management. [ABSTRACT FROM AUTHOR]

Published
2021
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40. Replacement of Rat Tracheas by Layered, Trachea‐Like, Scaffold‐Free Structures of Human Cells Using a Bio‐3D Printing System

Author

Machino, Ryusuke, primary, Matsumoto, Keitaro, additional, Taniguchi, Daisuke, additional, Tsuchiya, Tomoshi, additional, Takeoka, Yosuke, additional, Taura, Yasuaki, additional, Moriyama, Masaaki, additional, Tetsuo, Tomoyuki, additional, Oyama, Shosaburo, additional, Takagi, Katsunori, additional, Miyazaki, Takuro, additional, Hatachi, Go, additional, Doi, Ryoichiro, additional, Shimoyama, Koichiro, additional, Matsuo, Naoto, additional, Yamasaki, Naoya, additional, Nakayama, Koichi, additional, and Nagayasu, Takeshi, additional

Published
2019
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