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This study explored the effects of a novel intervention, binaural beats (BB), on anxiety levels of anatomy students during cadaveric dissections and its impact on the learning environment. The study was quasi‐experimental, employing a purposive sampling strategy. State (SA) and trait (TA) anxiety levels were measured using the State–Trait Anxiety Inventory during two routine dissection sessions. SA was measured at three time points: at the start, after a 3‐min control (silence)/intervention (10 Hz BB) session, and at the end of the dissection. A post‐study survey was distributed to the participants to obtain qualitative feedback on their perceptions of BB use during cadaveric dissections. Thirteen female students participated in this study (mean age 19.62 ± 0.65 years). Both control and intervention measures significantly reduced SA levels, with BB demonstrating a greater effect size (Hedges' g = 0.98 for intervention vs. 0.76 for control). SA decreased significantly after the BB intervention (t(12) = 3.78, p = 0.003) and remained low throughout the session, in contrast to the control session, where the reduction post‐measure was not sustained (t(12) = −0.64, p = 0.54). Feedback from the post‐study survey indicated that most students found the BB intervention effective in reducing their anxiety levels before the dissection. The BB intervention was effective in reducing and maintaining students' SA levels during dissection. Students reported positive experiences with BB. Further investigations are warranted to determine whether the findings of this study can be generalized to larger and more heterogeneous populations. [ABSTRACT FROM AUTHOR]