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The present study investigated the effects of replacing decapsulated cysts of Artemia urmiana in feed on larvae stage of Salmo trutta caspius in growth and survival indices. Experiment with six food groups treatment with 1: Control diet (without decapsulated Artemia cysts), treatment 2: Decapsulated Artemia cysts diet with 25% and 75% concentrate feed, treatment 3: Decapsulated Artemia cysts diet with 50% and 50% concentrate feed, treatment 4: Decapsulated Artemia cysts diet with 75% and 25% concentrate feed, treatment 5: Decapsulated Artemia cysts diet with 100%, treatments to 1 gram weight. In this experiment, the sequences of three parts, each containing 1500 larvae 120 with an average of 120+25 mg were used. During the test period the various parameters including weight gain rate larvae (GR), special growth rate (SGR), survival rate (SR), feed rate (FR) Feed conversion ratio (FCR) were analyzed. The results showed that among different groups, treatment 2 with 0.64% had the worst condition in terms of feed conversion ratio among treatments were tested. The results showed that the specific growth rate of treatments 2 with 0.55% had the best and treatment 4 with 0.34% had the worst condition. Effective growth rate in treatment 2 with 0.26% showed the best and treatment 5 with 0.17% showed the worst. Treatment 2with 154.31 the best and treatment 1 with 130.85 the worst effective feeding index. The highest average daily growth observed in treatment 2 and the lowest was observed in treatment 5. The highest obesity index was observed in treatment 2 with 0.96 and the lowest obesity was in treatment 5 with 0.85. In terms of survival, treatment 4 with 99.91% had the best result and treatment 5 with 99.62% had the worst result. According to the results of the present study, replacing 25% decapsulated Artemia cysts can be effective and useful in developing the most growth and survival indices of rainbow trout larvae. Iranian Fisheries Science Research Institute Published

Annually, More than 6 millions of cyprinid fish including Silver Carp( Hypophthalmichthys molotrix ) , Big head (Hypophthalmichthys nobilis ) , Grass carp( Ctenopharyngodon idella ) , Common carp( Cyprinus carpio)and Pike perch ( Stizostedion lucioperca) are produced in Poldasht Shahid Kazemi Propagation Center and released to water resources of West Azarbaijan to restocking purposes. Also, a large number of these fish are transported to the water sources and fish farms of the other parts of the country. Septicemia caused by motile Aeromonas species is considered as the famous disease in freshwater fish. It considered to cause of heavy losses in cyprinid farms of Iran in spring and summer, in recent years. On the other hand, Spring Viremia of Carp is considered as a sever pathogen of cyprinid fish in different parts of the world and has listed as notifiable diseases by OIE. This study was carried out to isolate and identify motile Aeromonas bacteria and SVCV in propagated cyprinid and lucioperca fish of Poldasht Shahid Kazemi Propagation Center. For this purpose, 40 fish of each species (totally 600 specimens), respectively with average weights of 500± 0.3 mg in incubation hall and 10±1 g in earthen ponds were sampled to determine bacterial infection. Samples from kidney, spleen, liver, blood, skin and gills were taken and cultured on BA, TSA, BHIA and NA and biochemical and differential tests were done. The bacteriological results represented the lowest percent of contamination with A.hydrophila in kidney and liver (1.5 ± 0.2%) at incubation stage and the highest (75.9±0.12%) during transferring to water resources. Also, Bacterial agents including Aeromonas sobria, Escherichia coli, Micrococcus,Staphylococcus, Aeromonas sp., Proteus mirabilis, P.aeruginosa, P.fluorescens and Pseudomonas putida were isolated and identified from sampled tissues. To isolate SVC virus with prevalence rate of 2%, 150 samples from 5 mentioned species were taken from kidney, spleen, gill and brain tissues (fish with 10±1 g weight and one year old fish) before transferring to the water resources. All samples were tested using RT-PCR for virological tests. The results showed the probability of SVC virus in one year old big head. Confirmation of the virus in accordance with international guidelines and the OIE requires additional testing and cell culture, the coordination of these tests with the National Veterinary Organization(IVOC) was carried out, which confirmed the failure to verify the results of definitive diagnostic tests .Finally The virus was not approved by the IVOC. Iranian Fisheries Science Research Institute Published

In order to study plankton population composition and species diversity in different regions of Aras Rivers and the evident reservoir sampling was performed monthly from determined sampling sites from April 2007 to March 2008. Sampling was conducted in Reservoir Lake by a PVC pipe with 2.25 m length and 5 cm diameter by columnar method. Besides, in more dipper (>5 m) sites, sampling conducted from bottom to surface with 1-m intervals (layer sampling) by a 2-liter Ruttner type sampler. In river due to water flow sampling was conducted by a scaled measure. Phytoplankton sampling was performed by 1-liter bottles. Zooplankton was sampled by filtering of 30 L through 55- micron plankton net. These samples were then transferred into bottles and sampling date and the name of station were attached on the bottles. Then, the samples were fixed immediately and transported to laboratory. It should be mentioned some physical parameters were determined in situ. Also, a 1liter and another 2-liter water were collected to determine chlorophyll a and chemical factors respectively. Samples were preserved in dark and cold place for a week. The upper layers (without phytoplankton) were removed and the lower layer (with phytoplankton) was sampled into 5-ml chambers. The enumeration and identification was performed by a Nikon TS100 inverted microscope with ×400 (Phytoplankton) and ×200 (Zooplankton) magnification according to Utermohl (1958) method. About 50 fields were enumerated for each sample. The comparison of physicochemical and biological factors with standard criterion indicated that the presence of a fertile plain, agricultural growth, drought and higher water demand in recent years, as well as, higher population density, the Nakhjavan city location and sewage water flows into the Aras reservoir, the lake is going to be an eutroph lake. Sampling of crayfish was performed from 5 sampling sites seasonally. Aras reservoir freshwater crayfish biomass was sampled in 1391, by putting 100 funnel-shaped nets. At each site one row nets composed of 20 nets were put by rocks, connecting robe and boat. Twenty four hours later, total freshwater cray fish was sampled (at least 300 crayfish was randomly sampled from each site). The samples were transported to laboratory and their sex, sex percentage, biometric characteristics including length, weight were recorded. Length and weight groups were estimated totally and for males and females in each season. The average total length and weight were 106.43 ± 7.94 mm and 35.81 ± 10.86 gr respectively which both are lower than West-Azarbaijan fisheries criterion. In this study only 18.99 percent contained a length higher than exporting level (120 mm). Yet, only 16.46 percent of harvesting had higher than 50 g (exporting level). With regard to data, freshwater shrimp (Astacus leptodactylus) in Aras dam reservoir stock has reduced. In general, male-female ratio was high. The comparison of growth equations confirms that males had higher weights than equal females. In recent years, the freshwater crayfish harvesting has been declined due to increased harvesting and over loading of nutrients into the lake.

Regarding the increase in world population and decrease in fish stock, there is an urgent need to aquaculture practices. Coldwater fish culture is aiming at production and providing a part of protein needs of the country and a way reaching to self- sufficiency and preserve the water resources. Recently, multi- purpose use of water resources potential and especially the rivers of west Azarbaijan, for example, construction of fish farms at upstream of permanent rivers in order to make optimum use of the resources and the increase in employment has been considered . The waste water of these pools is usually discharged into the rivers. Regarding to increased production per unit area due to water resources and cold water fish culture restrictions to use regional facilities and fish farm construction, it is nessacery to determine limits between 2 farms. In this study with the goal of evaluation of the impacts of trout farm effluents on water quality parameters of Pirdanan River, the factors of water current, temperature, pH, EC, TDS, TSS, DO, BOD5, NH3, N-NO2, N-NO3, TP P-PO4 according to standard methods were determined montly during 2014-2015 . Sampling sites were entrance, exit of Maroofi trout farm and the distances of 700, 1400, 2100 and 2800 m after the farm exit. Seasonal sampling of macro invertebrates from mentioned sites was conducted using stone leaching. The family and genus of macro invertebrates were identified using identification keys and abundance of macro invertebrate phylums for each site was determined. The results revealed that the values for water current, EC, TDS, TSS, N-NO3 were significant. the values of water temperature, BOD5, TDS, TSS, N-NO3- , N-NO2-, NH3, P-PO4- , NH4+, N-NH3 were increased in site 2 compare to site 1 in all months and gradually in the river and its self- purification were decreased. In this study, 4 phylums, 4 classes, 7orders and 14 families of macroinvertbrates were identified from Pirdanan River. Ephemeroptera had the highest abundance in site 4; while Diptera had the highest abundance in site 2 (exit). Regarding that most of Diptera macro invertebrates were from Chironomidae, so it can be concluded that site 2 was the most pollutant site and sites 5 and 6 were more suitable condition. Therefore, considering the physic- chemical factors and macro inverteberates indices, 2100 m from entrance, (site 5) is recommended compliance with veterinary organization rules and other conditions of aquatic engineering, environmental and water operation standards. Iranian Fisheries Science Research Institute Published

Sampling for this study was carried out at the freshwater crayfish fishing season (May to December ) from the selected packs made from one of the harvesting centers at the first day ( harvesting and packing ) until the seventh day ( the export time ) using registered number to determine the handling effect ( live ) and changes in chemical and microbial indices in tail fillet stored at -18 ° C at days 1 and every 15 days considering 1 treatment and 3 replicates. The comparison of results mean (±SE) of the effects of handling on tested indices showed a significant reduction of protein percentage (dry weight) of tail filet at day 7 (13/85 ± 0/07) comparing with fishing day (16/1 ± 0/05) ( (p < 0.05). So that by increasing the handling and storage period, the amount of protein (dry weight) was reduced. Comparison of carbohydrate percent (dry weight) during handling showed a significant difference between the first day (2/8 ± 0/009) with the seventh day (0/9 ± 0/005 (p 0.05). Comparison of the mean (±SE) of total bacterial count (log CFU / g) and psychrophilic bacteria at different periods of tail fillet storage at - 18 °C showed significant differences at day1 ( 3.48 ± 0.00 and 1.0± 0.00) compared to day 180 ( 6.86± 0.85 and 6.75± 0.25) , respectively(p>0.05) .Comparison of Mean (±SE) of chemical indices for first day with day 180 were thiobarbituric acid ( mg malondialdehyde /kg ) (0.19/0 ± 0/07) and (1/45 ± 0/25), peroxide ( meq oxygen/ kg fat ) (0/81 ± 0/21) and (2/2 ± 0/3), total volatile basic nitrogen ( mg per 100 g fillet ) (13/21 ± 1/01) and (26/6 ± 1 / 40) and acidity (6/26 ± 0/08) and (6/55 ± 0/05), respectively which showed significant differences with each other (p < 0.05). Therefore, cited to the results of microbiological and chemical parameters, maximum shelf life longetivity of freshwater crayfish tail fillets packaged in the air is recommended as 5 months after freezing at – 18 °C. Iranian Fisheries Science Research Institute Published

Stock assessment of Urmia Lake Artemia between 2000-2001 revealed intense decreasing cysts and biomass compared the previous years. Reducing annual rain falling from 32 to 18 cm in west Azarbayjan province lowered fresh water entrance from 4 billion m3 to 1.8 billion m3 to the lake as in 2001 the water volume reached to 6 billion m3 . Decreasing more than 2 billion m3 water volume of the lake per year with increasing the evaporation rate and existence of 5 billion tons salt inside the lake increased water salinity from 17% (1999) to 23.3% (2001) with max- min range of 250-258 ppt without any differences between the seasons. Mean transparency measured with Sechi disk was 138 cm (maximum 215 in Autumn and minimum 51 cm in Spring). Annual mean of water temperature 15 with fluctuation of 6 in winter to 22.13 in Autumn. this study, Artemia (different life stages) and phytoplankton were sampled by planktonic net from 12 suit stations which were selected from 36 sites used in previous Artemia stock assessment project done by Gent University, 1995. Phytoplankton sample identified and counted using identifying key and microscopic study. Results showed that in winter, the number of cysts per cubic meter 756 (minimum) and 2015 (maximum) in Autumn, with annual mean of 1000.m-3. Minimum 0.7 g.m-3 biomass wet weight (without cysts) were obtain in winter while the maximum (9.22 g.m-3) was in spring. The numbers of different nauplii stages per cubic meter with range of 0.03 in winter to 9.22 in spring( mean of 2.46) and juvenile stage per cubic meter were between 0.05 in winter to 1.16 in spring( mean= 0.66). It's showed higher percentage of larval stages in spring toward to higher juvenile and adults stages in summer to mid-autumn. Only Dunaliella and Nitzschia were identified but Dunaliella was dominated ( 95% of total identified phytoplankton with higher abundance, 31000 cells.L-1 in spring season(especially in May and June). Total biomass concluding cysts in studied year was 2700 tons( 30 tons cysts and 2670 tons other biomass which with 10% harvestable biomass,3 tons cysts and 267 tons other biomass could be harvested without any diverse effects on Artemia stock. Cysts and biomass were found more in central to southern part of the lake . The best season for cysts harvesting were Autumn follow by Spring and for biomass harvest were Summer follow by Autumn. Iranian Fisheries Science Research Institute Published

Qom Province with an area of 11,238 km^2 , surrounds about 0.68%of the country area and almost situated in the center of the country and enjoys a hot and dry climate . Because of the unique flora and fauna of the region especially the salt pools - hoze- Sultan and salt lakes enjoys a certain ecosystem in the country. Research has done in different parts of Iran and Qom province regarding the Artemia culture in salt lands that showed a great potential in the country and Qom province. The aim of this study that was held in order of Qom fisheries institute and environmental protection organization of that province and scientific leadership of Iranian Artemia Research Center, was the culture of Qom native Artemia and Artemia franciscana ( foreign species ) across Qom salt lake and determining the prevalence of cultured species in this region for this purpose, site selection and climatic and topographic data collection was held at 5 regions of Qom province. This regions were included margins of salt lake, and hoze sultan, shams abad drainage, railway station, and freshwater culture farm at the north east of Qom salt lake. margins of salt lake, and hoze sultan, were suitable for Artemia investment due to suitable climate and vast area, but they had problems in terms of technical facilities and access to areas . Also, the rail way station and plantation industry had dehydration problems. Therefore, after reviewing the field of agricultural water taken along the 45 km of Kashan and Qom Salt Lake were selected for the project because of the salty ecosystem similar to the lake, presence of salt water drainages downstream of the farm , technical facilities and a large area , soil texture and easy access to the area . For this purpose, six pools with 50 square meters near the reservoir pool available were constructed and 70 g native Artemia cysts of Qom and 23/3 g cysts of Artemia franciscana were hatched in Zooks of 10 liters for each pool ea of 50 square meters under standard condition (salinity 35 ppt pH =8, light lux 2000 and temperature 28°C). By calculating the useful volume of water for culture (112 cubic meters) and Hatching efficiency for native Artemia of Qom (40,000) and Artemia franciscana (120,000), the number of 25 nauplii per liter equal to the 2.8 million nauplii were released to each pool with size 4 × 12 m and high slope with area of 50 meter in the floor and 273 meters in the surface . Non- biological and biological studies, including algae and water analysis and population assessments were performed regularly in pools every 2 weeks. the culture period were from July to mid- October, 2009 for a period of 3/5 months. The results of algal study revealed more abundance of Cholestrium, Navicula, Nitzshia algae during culture period (105 days), that Amphora, etraselmis, Cymbella, Senedesmus, Dscillatoria, Gomphonema genera were observed, too. The results of water parameters analysis revealed that they should preserve at salinity 40–50 ppt, pH 8, water hardness 7000- 9000 mg. the results of population assessment showed a larger amounts of female broods of Qom Artemia at days 15,30 and 90 of culture with average number of 120.3, 68.8 and 29.5 per liter, respectively, comparing to Artemia franciscana with average number of 28.8, 17.5 and 7.7 per liter that was significant (p

The comparative study of the streptococcinum, Hepar sulfur, Rosmarinus officinalis and erythromycin effects on cultured rainbow trout (Oncorhynchus mykiss) with experimental streptococcosis Homeopathy is one of alternative medicines that is very useful for soul and body diseases with accurate prescription. The goal of this study was “survey of the effects of homeopathic remedies. There is not any more research about homeopathy on aquatics in the world especially in Iran, thus Some research about the effects of homeopathy on aquatics is needed. In this study, the effects of streptococcinum, Hepar sulfure, Rosmarinus officinalis(homeopathic remedies) and erythromycin in cultured rainbow trout, with experimental streptococcosis, also the mortality, were compared. There was 6 treatment and 2 reviews in 300 liter tanks that each of treatment contained 40 juvenile rainbow trout with 25±5 gr arrange weight. Treatment 1: contained of erythromycin. Treatment 2: Streptococcinum C30. Treatment 3: Rosmarinus officinalis Q. Treatment 4: Hepar sulfur C30 . Treatment 5:(control treatment )without any injection and any therapy. Treatment 6: (positive control treatment) with injection but without any therapy. Daily estimation of the water temperature, oxygen, pH and salinity and some other chemical factors. Tretment 1 had significance with the other treatments. Survival percent in treatments and their analysis showed that treatment 4(Hep-s) had significance (p

Early recognition of diseases and pathogens transmitted from freshwater crayfish provides the possibility of early intervention and management in this regard and decreases the mortality and morbidity from infectious diseases. Hence, The awareness from presence and incidence rates of WSSD, YHD, IPN viruses are very important due to they are the most important zoonotic and infectious viruses in aquatic animals and crustaceans .Also , they cause serious reduction of the amount and quality of freshwater crayfish in the world. For this region, this study was carried out for the first time to detect the mentioned viruses in freshwater crayfish from North West of Iran. In this study, a total of 725 crayfish (145 specimens from each water resource) were sampled during 2013 -2014 from dam reserviors including : Aras ( West Azarbaijan, Caspian Sea Basin),Ardalan ( East Azarbaijan, Urmia lake basin) , Kazarj and karaghyeh (East Azarbaijan, Caspian Sea Basin),Shorabil(Ardabil, Caspian Sea Basin).All specimens were transferred to aquatic animals health and genetics laboratory of Iranian Artemia Research Center as live individuals under controlled condition. In order to virus isolation, the gill, Hepatopancreas, Hemolymph and pleopods were tested using PCR and RT-PCR methods. Suspicious samples were sent to the reference viral diseases diagnosis laboratory of Iranian veterinary organization.Screening results showed the probability of no contamination in freshwater crayfish from studied water resources. Therefore, it is recommended continuation of screening mentioned viruses by the authorities placed on the agenda. Iranian Fisheries Science Research Institute Published

Monitoring of Artemia Stock in Urmia Lake ( with less than 5 billion m^3 ), between 2001-2003 in order to survey of Artemia population dynamic and some water abiotic factors such as WT , salinity and transparency using Thermometer, refractometer and secchi disk, were done monthly. Phytoplankton with Rotner sampling device and Artemia (different life stages) were sampled by planktonic net from 7 suit stations(3 layers, surface, 2.5 and 5 meters with 3 replications) which were selected from 12 sites used in previous Artemia stock assessment project Graphs drawed by excel software in different layers , months and separation between north and southern parts of the Lake(±SD ,SE), showed WT fluctuated from 3 degree centigrade in December 2001 to 29 degree in November 2003 with the same changes in different layers. Salinities from 290 ppt in autumn 2001 to 340 ppt in summer of 2003 increased. Northern part of the lake had higher salinity than the southern. There were not any differences between different layers in salinity changes, transparency were as the depth of water and there were no any phytoplankton in samples. Number of cysts in surface was 1933±850, in northern part 1782±998 and in southern 2032±1061. At 2.5 meter depth 327±110T 229±107 and 363±110 cysts were counted respectively. At 5 meter depth there were 419±124, 181±62 and 282±65. Other numbers of biological stages were counted as cysts in three layers, north and southern parts of the lake. Data concluded that cysts and biomass were dominated in southern part but at surface and 2.5 meter depth respectively.

Today, due to population increase and anthropogenic activities together with sewage and agricultural waste water entrance, aquatic ecosystems have been exposed to high pollutions. Phytoplankton is a group of water floating algae that have crucial roles in providing nutrients and oxygen for other organisms, nitrogen and CO_2 fixation. These organisms are considered as primary producers in aquatic ecosystems. They are found in various water habitats all over the world, affected by environmental variables such as pH, light and temperature and used for determination of water pollution degree and quality. Phytoplankton composition and density may be used as a complementary indicator of water trophy state. Phytoplankton communities indicate short and long term variations of aquatic systems. One of the most obvious problems in freshwater ecosystems is algal bloom or over growth of some blue- green algae which can decrease oxygen, and in some cases bring about toxin excretion and fish and human kills. In addition, many of bloom forming cyanobacteria produce secondary metabolites which can create sever poisoning in mammals including human. The genus Microcystis is a key bloom forming cyanobacteria in aquatic ecosystems. Populations of this genus form intense blooms in water bodies that has attracted more attentions in recent years. Various species of this alga have been distributed in stagnant and eutroph freshwater around the world. Microcystis has been defined by genetic criteria such as 16S rRNA molecular sequencing, but its classification in levels lower than genus is unclear and the presence of its classical morphospecies is doubtful. However, this genus creates sever blooms in eutrophic waters all over the world and many species produce toxins. Therefore, identification of its natural diversity in the levels lower than genus has high importance. However, several characteristics of Microcystis morphotypes which are classified a traditional species, actually are present and observed in different regions of the world. At present, they can be considered as morphospecies that belong to one genotype and have similar ecology. These traditional species with definite phenotypic and ecophysiological characteristics cannot be eliminated completely. Their identification is essential for ecological and ecotoxicological studies. Interistic transcribed separator (ITS) gene is a section of genome which is located between 16s rRNA and 23s genes. This gene has more heterogeneity than 16s rRNA; so it is used to identify many genera of cyanobacteria. The Aras reservoir located in the north-west of Iran plays important roles as fisheries, drinking and agricultural water supply and recreational activities in the region. The present thesis was undertaken to: analyze the Microcystis sp. populations by molecular methods such as ITS in Aras Reservoir. Samples for molecular analysis were collected from 10 sampling sites on 18 August 2013. Samples for molecular study of Microcystis were collected from two different depths (surface and 1 m depth) and transferred to laboratory without any treatments. Microscopic images of Microcystis were sent to professor Komárek and was confirmed.

Artemia as a live food has multiple applications in aquaculture. Artemia contains few unsaturated 3-omega fatty acids particularly eicozapentanoeic acid (EPA) and has no 6-omega fatty acids particularly decozahexanoeic acid (DHA), so Artemia naplious is enriched to improve its food values. The most famous enrichment emulsion are selco and super-selco made by Euro-American INVE Company. This study was performed to make Artemia enrichment emulsions by internal potentials. At first, the final composition of Artemia enrichment emulsion (selco) was determined in Urmia university chemical analysis laboratory. Then, aquatic fishing resources in south of the country such as eye oil of tuna fish, shark liver, cuttlefish and plant oils of sunflower, olive and beef oil were used. Fatty acids profiles were analyzed by Gas Chromatography (GC). The results showed that cuttlefish may produce 13± 3 % wet weight of fatty acids. We made 3 enrichment oils which contained 60± 10 % of plant and animal oils. These suspensions fatty acids were analyzed and the results were compared with control sample. Field tests were performed on 200000 Artemia urmiana naplious by enriching the Artemia with enrichment emulsions and the products were analyzed by GC. The results indicated that the rate of emulsions absorbance in imported and internal samples were 37.47, 25.30, 18.88, 22.14 and 10.32 respectively. In the next stage, enriched Artemia naplious were fed as live food to 500 trout larvae in Ziveh Aquaculture Company as follows: Treatment 1- Control consisted of new feeding larvae fed by concentrated food Treatment 2- new feeding larvae fed by concentrated food plus un-enriched naplious Treatment 3- new feeding larvae fed by concentrated food plus selco oil enriched naplious Treatment 4- new feeding larvae fed by concentrated food plus emulsion-1 oil enriched naplious Treatment 5- new feeding larvae fed by concentrated food plus emulsion-2 oil enriched naplious Treatment 6- new feeding larvae fed by concentrated food plus emulsion-3 oil enriched naplious The results indicated that treatments 1, 2 had significant difference with treatments 3, 4, 5, 6 from survival rate, growth coefficient, obesity coefficient, total length, food conversion coefficient, final weight and protein percent. Abnormalities rates in treatments 1, 2 had significant difference with treatments 3, 4, 5 , 6 in which enriched emulsions were not used, but these indices had no significant difference with commercial samples which shows internal made emulsions can easily be used. The data were analyzed by one-way analyses variance and Duncan test in SPSS and EXCELL softwares. In conclusion, we can make enrichment selco oils in the country by internal potentials which the foreign samples can be replaced by them.

Saltwater resources with Artemia (Brine shrimp) are the important ecosystems in Semi-arid Areas. That can play an important role in sustaining the economic and social system. Alternatively, will ensure that economic and social issues with of continuity and sustainability of these ecosystems resident. This study carried out "Effects of Artemia parthenogenetica culturing on socio-economic development of the eastern marginal lands, Qom Salt Lake" that was ordered by Qom province general directorate of fisheries during the 2012-2013. According to previous studies, despite the potential of human resources and Social conditions for the development of economic activities, factors such as the low groundwater and drying the salt lake , lack of fresh water in the region, environmental problems , successive droughts, prioritize and rationalize water use in agriculture, Artemia cyst harvest or Artemia culture has made virtually impossible with the current situation and has deprived the tools to make investments for the construction of earthen ponds and exploitation of native Artemia in the eastern margin of the Qom lake . Also, due to the occurrence of the Lake in the national park and privacy protection and Prohibited areas of environmental protection organization, it seems unlikely that this organization grant approval agreement required for any exploitation. Iranian Fisheries Science Research Institute Published

Early recognition of diseases and pathogens transmitted from freshwater crayfish provides the possibility of early intervention and management in this regard and decreases the mortality and morbidity from infectious diseases. Hence, The awareness from presence and incidence rates of WSSD, YHD, IPN viruses are very important due to they are the most important zoonotic and infectious viruses in aquatic animals and crustaceans. Also they cause serious reduction of the amount and quality of freshwater crayfish in the world. For this region, this study was carried out for the first time to detect the mentioned viruses in freshwater crayfish from North West of Iran. In this study, a total of 725 crayfish (145 specimens from each water resource) were sampled during 2013 -2014 from dam reserviors including : Aras ( West Azarbaijan, Caspian Sea Basin),Ardalan ( East Azarbaijan, Urmia lake basin), Kazarj and karaghyeh (East Azarbaijan, Caspian Sea Basin),Shorabil(Ardabil, Caspian Sea Basin).All specimens were transferred to aquatic animals health and genetics laboratory of Iranian Artemia Research Center as live individuals under controlled condition. In order to virus isolation, the gill, Hepatopancreas, Hemolymph and pleopods were tested using PCR and RT-PCR methods. Suspicious samples were sent to the reference viral diseases diagnosis laboratory of Iranian veterinary organization. Screening results showed the probability of no contamination in freshwater crayfish from studied water resources. Therefore, it is recommended continuation of screening mentioned viruses by the authorities placed on the agenda.

Aras dam reservoir situated in the northwest of Iran, west Azarbaijan province, is the only water resource of Astacus leptodactylus harvest in the country that more than 250tons of this species were exported to different countries all over the world, annually. On the other hand, one of the polices of Iranian Science Fisheries Institute is the release of this species into other water resources in the country and for this purpose, the study of risky diseases such as Crayfish pest ( Aphanomysis astasi ) and other zoonotic diseases are considered as the research priorities of aquaculture development of the country. This study was carried out to health screening of Astacus leptodactylus at Aras dam reservoir from winter 2013 to fall 2014. In this regard, A total of 394 harvested livefreshwater crayfish Astacus leptodactylus (255males, 139females) weretested. 9 epibionts and parasites peritrich protozoans were identified. From Metazoan parasites group, Branchiobdella kozarovi with incidence rate of (100%) in obtained samples was the only isolated organism from this group that identified up to species level. There was a heavy damage in gills of samples with Aeolosoma hemprichi (Annelid) in winter with90% prevalence. Furthermore, Other Epibiont fouling organisms such as Rotatoria; free living nematods and suctoria were observed in this survey. The fungi study of the lesions and melanized spots of mentioned samples revealed their infection to Penicillium expansum; Aspergillus flavus; Alternaria sp. ; Fusarium sp. and Saprolegnia sp. The results of bacterial study confirmed the presence of pathogen bacteria in Astacus leptodactylus. The most frequency percentage ( 15.16%) in hepatopancrease were related to Aeromonas hydrophila and the least one ( 1.37%) were due to Yersinia bacteria . Also, only Aeromonas hydrophila and Staphylococcus aureus were isolated and identified from heamolymph, respectively. The results revealed that the combination of Salmonella typhi, Escherichia coli and Staphylococcus sp. has caused the most infection rate while. Yersinia ruckeri and Salmonella typhi has caused the least infections in Astacus leptodactylus. According to the isolation of 6 bacteria species from hepatopancreas and 2 species from heamplymph , it can be concluded that hepatopancreas enjoyed the higher infection rate compared to haemolymph in the obtained samples. Iranian Fisheries Science Research Institute Published

This research was carried out to determine water reuse effects on growth indices of rainbow trout. For this reason 4 groups including: Control (with use of 100% fresh water), Test 1(rearing with use of 30% recycled water (with physical filtration and 10% fresh water), Test 2 (using of 10% recycled water (with physical filtration and 30% fresh water), Test 3(using of 100% recycled water) was done. Fish stock of each groups were done with 62 fish in each square meter and average (±SD) weight (15±1.5). The Growth index, SGR, CF, FCR, SR between different groups in each month was calculated and determined. In this research comparing the average of monthly growth increasment between different groups indicated that significant decrease of growth in test groups with control group as well as groups 1 and 2 with 3 groups. There were no significant differences in this factor between groups 1 and 2. FCR, SR and MR showed a significant difference between different groups. These results showed with increasing the replacement of water in rainbow trout culture ponds with recycled water cased significant decrease in SR and CF and significant increase in MR and finally decrease in production and density per unit area.

The brine shrimp Artemia consists of a number of bisexual species and a large number of parthenogenetic forms, which collectively, inhabiting a wide range of hypersaline habitats. This research was carried out in order to study the genetic differentiation of Artemia urmiana and two parthenogenetic populations originated from surrounding lagoons of the Lake Urmia (Fesendoz, Saray village) and also to find the phylogenetic relationships of some Iranian Artemia (Qom, Arak, Golestan, and Shiraz) with a comparison to some foreign Artemia species (A. sinica, A. franciscana, A. parthenogenetica (Russia and Turkmenistan) using a molecular technique (PCRRAPD`s). Of a total number of 56 random primers (10 bp) survey, 29 and 26 oligonucleotides showed polymorphic patterns with highly reproducible bands in Artemia urmiana groups and Iranian Artemia groups.The highest percentage of polymorphic loci were in Artemia urmiana (P= 78.89 %) and in comparison with the other parthenogenetic forms, in parthenogenetic Artemia from Saray village (P= 59.8 %). The highest values of Shannon index were estimated in Artemia urmiana (I= 0.41), and in comparison with other parthenogenetic forms, in parthenogenetic Artemia from Saray village (I= 0.3). Analysis of molecular variance indicated that most variation were within populations (A. urmiana with parthenogenetic forms, FST= 0.35) and among populations (Iranian Artemia with foreign species, FST= 0.76). Genetic pattern was not in accordance to geographical distribution, which indicated the wide range of Artemia samples at different regions in Iran. UPGMA showed two distinct clusters in group I including A. urmiana with two parthenogenetic forms and four clusters in group II including Iranian Artemia with some foreign species. It is understood that two parthenogenetic populations (Fesendoz and Saray village) had close genetic relationship together with high value of heterozygosity in parthenogenetic Artemia from Saray village. Iranian Artemia (Qom, Arak, and Golestan) indicated close genetic relationships with A. parthenogenetica (Russia and Turkmenistan) with low values of heterozygosity. A sister group was generated including Shiraz and USA Artemia samples which attests a possible contamination of local water with A. franciscana. FST value revealed high genetic structuring at all studied groups. Besides, it is suggested that gene flow was not strong enough to prevent substantial genetic differentiation. Result could lead to a management programmes to use suitable Artemia strains with high genetic diversity, for culturing and establishment of gene bank of Artemia stocks in Lake Urmia. Iranian Fisheries Science Research Institute Published

Background: The most common drug poisoning in Iran, especially in Tehran, is poisoning with tramadol which is a synthetic analgesic. Like poisoning with other opioids, poisoning with tramadol is commonly treated using naloxone. As documented in poisoning references, seizure is a serious and dangerous side effect of this poisoning. The aim of this study was to compare the prevalence of seizure between patients with tramadol overdose who received or did not receive naloxone in Imam Khomeini Hospital, Ardabil, Iran. Methods: In this cross-sectional, descriptive study during 2007-10 data about 122 patients with tramadol overdose in Imam Khomeini Hospital was collected using a questionnaire. The collected data was analyzed in SPSS. Findings: Males constituted 89.3% of the participants (n = 109). The mean age of subjects was 27.0 ± 7.2 years. Seizure was observed in 21 persons (17.2%). No significant relationship existed between seizure and sex, age, and history of addiction to tramadol and other opioids (P > 0.05). Among the 60 subjects who received naloxone, 28.3% had seizure. The prevalence of seizure in persons who did not receive naloxone was 11.2%. The highest prevalence of seizure caused by naloxone was seen during the first 1.5 hours after injection. The lowest dose of naloxone that caused seizure was 1000 mg. Conclusion: Seizure caused by tramadol had no relationship with sex, age, and history of addiction to tramadol or other opioids. Moreover, seizure occurrence is rare in therapeutic dosage. Using naloxone in treatment of tramadol overdose increases the risk of seizure. Keywords: Tramadol, Naloxone, Seizure, Intoxicated

Injection drug use has been the most growing rout of drug abuse in Iran in the past decade and it has been responsible for the transmission of HIV virus in more than two third of cases. The aim of the present study was to determine the prevalence of HIV and hepatitis B in a group of IDU cadavers and to compare the results to a group of cadavers of the normal population. In a case-control study the blood samples of the cadavers of 400 randomly chosen IDUS and 400 other cadavers as control group were checked for HBS antigen and Anti HIV antibody in the forensic medicine center of Tehran. The prevalence of HIV and HBV infection was compared in two groups according to their demographic characteristics. The number of HIV and HBV positive cadavers was significantly higher in the IDU group than the controls (6.25% vs 0.5%, P