131 results on '"Motegi SI"'
Search Results
2. Predictors of clinical features in early-onset severe systemic sclerosis: An analysis from a multicenter prospective observational Japanese cohort.
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Uesugi-Uchida S, Fujimoto M, Asano Y, Endo H, Goto D, Jinnin M, Kawaguchi Y, Tanaka S, Tokunaga T, Makino K, Matsushita T, Motegi SI, Yoshizaki A, Sato S, and Hasegawa M
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- Humans, Male, Female, Japan epidemiology, Prospective Studies, Middle Aged, Adult, Prognosis, Vital Capacity, Scleroderma, Systemic diagnosis, Scleroderma, Systemic immunology, Scleroderma, Systemic pathology, Scleroderma, Systemic complications, Scleroderma, Systemic blood, DNA Topoisomerases, Type I immunology, Skin pathology, Age of Onset, Skin Ulcer diagnosis, Skin Ulcer etiology, Skin Ulcer pathology, Follow-Up Studies, Scleroderma, Diffuse diagnosis, Scleroderma, Diffuse complications, Scleroderma, Diffuse immunology, Scleroderma, Diffuse pathology, Disability Evaluation, East Asian People, Disease Progression, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial immunology, Lung Diseases, Interstitial etiology, Severity of Illness Index
- Abstract
As the clinical course of systemic sclerosis (SSc) varies widely, prognostic indicators have been sought to predict the outcomes of individual patients. Racial differences in SSc render it necessary to validate prognostic indicators in different patient cohorts. In this study, we aimed to assess clinical and laboratory parameters in Japanese patients with early-stage SSc with diffuse cutaneous involvement and/or interstitial lung disease, and identify predictive factors for disease progression. We performed multivariate analyses of baseline clinical information to estimate symptoms 4 years later in Japanese patients with diffuse cutaneous SSc and/or SSc with interstitial lung disease. Patients were enrolled in the study within 5 years of disease onset at 10 Japanese SSc centers. Over 12 years, 115 patients followed up for 4 years were included in this study. The modified Rodnan skin score (mRSS) at 4 years correlated with the baseline mRSS and finger-to-palm distance, defined as the average length from the distal tip of the fourth finger to the distal palmar crease. The percentage predicted vital capacity (%VC) in year 4 positively and negatively correlated with initial %VC and the presence of anti-topoisomerase I antibodies, respectively. The Health Assessment Questionnaire Disability Index (HAQ-DI) at 4 years was positively and negatively associated with baseline HAQ-DI and %VC, respectively. The occurrence of digital ulcers within 4 years was associated with the initial presence of digital ulcers, finger-to-palm distance, and the presence of digital pitting scars and anti-topoisomerase I antibodies. This study identified several factors that may predict the progression of early-stage SSc in Japanese patients. Finger-to-palm distance may be a useful tool for predicting the progression of skin thickening and the development of digital ulcers in the early stages of severe SSc, but larger, long-term prospective studies are needed to confirm our findings., (© 2024 The Author(s). The Journal of Dermatology published by John Wiley & Sons Australia, Ltd on behalf of Japanese Dermatological Association.)
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- 2024
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3. Recommendations for cancer screening and surveillance in patients with Werner syndrome.
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Aono K, Maezawa Y, Kato H, Kaneko H, Kubota Y, Taniguchi T, Oshitari T, Motegi SI, Nakagami H, Taniguchi A, Watanabe K, Takemoto M, Koshizaka M, and Yokote K
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- Humans, Male, Female, Aged, Middle Aged, Werner Syndrome diagnosis, Werner Syndrome complications, Early Detection of Cancer methods
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- 2024
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4. Exposure to volatile ferroptosis inhibitor, TEMPO, reduced cutaneous ischemia-reperfusion injury progression to pressure ulcer formation in a mouse model.
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Ishikawa M, Uchiyama A, Kosaka K, Nishio M, Ogino S, Yokoyama Y, Torii R, Akai R, Iwawaki T, Torii S, and Motegi SI
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- Animals, Humans, Male, Mice, Apoptosis drug effects, Disease Models, Animal, Disease Progression, Fibroblasts drug effects, Fibroblasts metabolism, Fibroblasts pathology, Mice, Inbred C57BL, Oxidative Stress drug effects, Skin pathology, Skin drug effects, Skin blood supply, Cyclic N-Oxides pharmacology, Ferroptosis drug effects, Pressure Ulcer pathology, Pressure Ulcer drug therapy, Pressure Ulcer etiology, Reperfusion Injury pathology, Reperfusion Injury drug therapy, Reperfusion Injury metabolism
- Abstract
Background: Ischemia- reperfusion (I/R) injury-induced oxidative stress is a key factor in the pathogenesis of pressure ulcer formation. Ferroptosis is an iron-dependent programmed cell death that connects oxidative stress and inflammation in various diseases. Recent studies revealed the protective effect of inhibition of ferroptosis in I/R injury. However, the role of ferroptosis in cutaneous I/R injury remains elusive., Objective: To assess the role of ferroptosis in the progression of cutaneous I/R injury., Methods: Cutaneous I/R injury experiments and histopathological studies were performed in wild-type mice with or without exposure to volatile ferroptosis inhibitor, TEMPO (2,2,6,6-Tetramethylpiperidine-1-oxyl). The suppressive effects of TEMPO on ferroptosis inducing cell death and oxidative stress were examined in vitro., Results: Inhibition of ferroptosis with TEMPO significantly reduced ulcer formation after cutaneous I/R injury. Fluctuated ferroptosis markers, such as GPX4, ACSL4, and 4-HNE expression in the I/R skin site, were reversed by TEMPO treatment. Inhibition of ferroptosis reduced apoptosis, CD3
+ infiltrating lymphocytes, and improved vascularity in the I/R skin site. Inhibition of ferroptosis also suppressed the enhancement of Nrf2 activation. In vitro, ferroptosis and the activation of ferroptosis-related gene expression by RSL3 stimulation were markedly ameliorated by TEMPO treatment in mouse fibroblasts. Inhibiting ferroptosis also suppressed the elevation of the mRNA levels of NOX2 and HO-1 caused by ferroptosis., Conclusion: Cutaneous I/R injury-induced ferroptosis likely promotes cell death, vascular loss, infiltration of inflammatory cells, and oxidative stress. The inhibition of ferroptosis with TEMPO might have potential clinical application as novel therapeutic agent for cutaneous I/R injury., Competing Interests: Conflict of Interest The authors have no conflicts of interest to declare., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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5. Comparative B cell epitope profiling in Japanese and North American cohorts of MDA5+ dermatomyositis reveals a direct association between immune repertoire and pulmonary mortality.
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Yamaguchi K, Poland P, Zhu L, Moghadam-Kia S, Aggarwal R, Maeno T, Uchiyama A, Motegi SI, Oddis CV, and Ascherman DP
- Abstract
Objectives: Anti-melanoma differentiation-associated gene 5 antibody-positive (MDA5+) dermatomyositis patients exhibit clinical features that vary by geographical and ethnic/genetic distribution. We therefore investigated whether B cell epitope profiles and corresponding clinical features distinguished two independent cohorts of MDA5+ dermatomyositis., Methods: We used ELISA-based methods to determine the relationship between antibody recognition of overlapping 155 amino acid MDA5 subfragments and clinical features of 17 MDA5+ dermatomyositis patients from Japan. Associations between clinical features and standardized anti-MDA5 subfragment antibody titers were assessed via Brunner Munzel testing and compared with clinical/serological profiles of an independent North American cohort. ROC analyses and Kaplan-Meier curves were used to further assess the relationship between anti-MDA5 fragment antibody levels and specific clinical features/outcomes., Results: Clinical characterization of a Japanese cohort of 17 MDA5+ dermatomyositis patients revealed a high prevalence of arthritis (47%) and interstitial lung disease (ILD) (100%). Serological profiling demonstrated predominant antibody recognition of MDA5 fragments A (aa 1-155), B (aa 130-284), and E (aa 517-671) in a pattern that was distinct from North American MDA5+ patients (n = 24) whose sera preferentially recognized fragment H (aa 905-1026). Statistical analysis revealed a striking association between anti-fragment A antibody levels and rapidly progressive ILD (RP-ILD) among Japanese patients (p< 0.01). ROC and Kaplan Meier curves also demonstrated a strong relationship between anti-fragment A antibody levels, RP-ILD, and pulmonary death in combined cohort analyses., Conclusions: Japanese and North American MDA5+ dermatomyositis patients manifest markedly different B cell epitope profiles that are associated with higher prevalence of RP-ILD and worse clinical outcome among Japanese patients., (© The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
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- 2024
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6. Possible effectiveness of switching biologics from adalimumab to certolizumab pegol in pyoderma gangrenosum.
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Kimura M, Uchiyama A, Endo Y, Yasuda M, and Motegi SI
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- 2024
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7. Real-world effectiveness and safety of abrocitinib in 12 Japanese patients with atopic dermatitis and transcriptome analysis with peripheral blood.
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Uchiyama A, Kosaka K, Ishikawa M, Inoue Y, and Motegi SI
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- Adult, Female, Humans, Male, Middle Aged, Young Adult, Boron Compounds adverse effects, Boron Compounds therapeutic use, Cytokines blood, Cytokines metabolism, East Asian People, Japan, Pyrimidines adverse effects, Pyrimidines therapeutic use, Sulfonamides adverse effects, Sulfonamides therapeutic use, Transcriptome, Treatment Outcome, Dermatitis, Atopic blood, Dermatitis, Atopic diagnosis, Dermatitis, Atopic drug therapy, Dermatitis, Atopic genetics, Gene Expression Profiling, Severity of Illness Index
- Abstract
Atopic dermatitis (AD) is a common chronic inflammatory skin disease characterized by recurrent, pruritic, and localized eczema. Various types of new drugs have been recently investigated for treating AD. The efficacy and safety of abrocitinib in treating AD has been reported in clinical trials, but the real-world data from Japan has not been reported. Herein, we analyzed 12 Japanese patients with AD treated with 100 mg of abrocitinib using our real-world data. We also performed transcriptome analysis with peripheral blood to investigate the effects of abrocitinib on cytokine expressions and inflammatory pathways in AD from three patients. This study included patients with moderate to severe AD treated with abrocitinib at Gunma University Hospital, Japan. All patients were systemic treatment-naïve. All patients received a 100-mg dose of abrocitinib daily, and used strong or very strong topical steroids and moisturizers. The Eczema Area and Severity Index (EASI) response analysis revealed that after 4 weeks, 25% (three of 12) of the cases reached a 75% reduction in the EASI score (EASI-75) and a 90% reduction in the EASI score (EASI-90). After 12 weeks, 83.3.% (10 of 12), 41.6% (five of 12), and 16.7% (two of 12) of the patients reached EASI-50, a 75% reduction in the EASI score (EASI-75), and EASI-90. Peak Pruritus Numerical Rating Scale was achieved in nine patients (75%) at week 12. The most frequent adverse reaction was acne (six cases [50%]). Gene Ontology pathway analysis using Differentially expressed genes from RNA sequencing analysis revealed attenuation of defense responses to biotic stimulus, virus, and cytokines. Th2 cytokine expression was not suppressed, but several chemokines, especially CXCL1, were suppressed by abrocitinib treatment. Our results indicate abrocitinib as a fast-acting and highly antipruritic agent that is effective for moderate skin eruptions., (© 2024 The Authors. The Journal of Dermatology published by John Wiley & Sons Australia, Ltd on behalf of Japanese Dermatological Association.)
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- 2024
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8. Case report of anti-survival motor neuron complex antibody-positive overlap syndrome of diffuse cutaneous systemic sclerosis and idiopathic inflammatory myopathies.
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Saito M, Uchiyama A, Kim J, Endo Y, Yasuda M, Aoki S, Ikeda Y, Nishino I, and Motegi SI
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- Humans, Female, Autoantibodies blood, Autoantibodies immunology, Middle Aged, Motor Neurons pathology, Motor Neurons immunology, Syndrome, Male, Myositis immunology, Myositis diagnosis, Myositis pathology, Myositis complications, Scleroderma, Diffuse immunology, Scleroderma, Diffuse complications, Scleroderma, Diffuse diagnosis, Scleroderma, Diffuse pathology
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- 2024
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9. Exacerbated Inflammatory Gene Expression After Impaired G2/M-Checkpoint Arrest in Fibroblasts Derived From a Patient Exhibiting Severe Adverse Effects.
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Oike T, Okuda K, Haruna S, Shibata A, Hayashi R, Isono M, Tateno K, Kubo N, Uchiyama A, Motegi SI, Ohno T, Uchihara Y, Kato Y, and Shibata A
- Abstract
Purpose: Recent radiation therapy (RT), such as intensity modulated radiation therapy and particle RT, has improved the concentration of the radiation field targeting tumors. However, severe adverse effects still occur, possibly due to genetic factors in patients. We aimed to investigate the mechanism of exacerbated inflammation during RT., Methods and Materials: Dermal fibroblasts derived from a patient with severe inflammatory adverse effects during RT were compared with 2 normal human dermal fibroblasts. Micronuclei formation, G2/M-checkpoint arrest, DNA damage signaling and repair, and inflammatory gene expression were comprehensively examined., Results: We found greater micronuclei formation in radiation-sensitive fibroblasts (RS-Fs) after ionizing radiation (IR). RS-Fs exhibited premature G2/M-checkpoint release after IR, which triggers micronuclei formation because RS-Fs undergo mitosis with unrepaired DNA double-strand breaks (DSBs). Additionally, we found that DSB end-resection and activation of the ATR-Chk1 pathway were impaired in RS-Fs after IR. Consistent with the increase in the formation of micronuclei, which can deliver cytosolic nucleic acids resulting in an innate immune response, the expression of genes associated with inflammatory responses was highly upregulated in RS-Fs after IR., Conclusions: Although this is a single case of RT-dependent adverse effect, our findings suggest that impaired G2/M-checkpoint arrest due to the lack of DSB end-resection and activation of the ATR-Chk1 pathway causes exacerbated inflammation during RT; therefore, genes involved in G2/M-checkpoint arrest may be a predictive marker for unexpected inflammatory responses in RT., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 Published by Elsevier Inc. on behalf of American Society for Radiation Oncology.)
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- 2024
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10. A case of autoimmune pulmonary alveolar proteinosis during the course of treatment of rapidly progressive interstitial pneumonia associated with anti-MDA5 antibody-positive dermatomyositis.
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Yatomi M, Akasaka K, Sato S, Chida M, Kanbe M, Sawada H, Yokota I, Wakamatsu I, Muto S, Sato M, Yamaguchi K, Miura Y, Tsurumaki H, Sakurai R, Hara K, Koga Y, Sunaga N, Yamakawa H, Matsushima H, Yamazaki S, Endo Y, Motegi SI, Hisada T, and Maeno T
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- Female, Humans, Middle Aged, Autoantibodies, Cyclophosphamide therapeutic use, Interferon-Induced Helicase, IFIH1, Pulmonary Alveolar Proteinosis complications, Pulmonary Alveolar Proteinosis diagnosis, Pulmonary Alveolar Proteinosis drug therapy, Dermatomyositis complications, Dermatomyositis drug therapy, Lung Diseases, Interstitial complications, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial drug therapy, Dermatitis complications, Autoimmune Diseases
- Abstract
Background: Autoimmune pulmonary alveolar proteinosis (APAP) is a diffuse lung disease that causes abnormal accumulation of lipoproteins in the alveoli; however, its pathogenesis remains unclear. Recently, APAP cases have been reported during the course of dermatomyositis. The combination of these two diseases may be coincidental; however, it may have been overlooked because differentiating APAP from a flare-up of interstitial pneumonia associated with dermatomyositis is challenging. This didactic case demonstrates the need for early APAP scrutiny., Case Presentation: A 50-year-old woman was diagnosed with anti-melanoma differentiation-associated gene 5 (anti-MDA5) antibody-positive dermatitis and interstitial pneumonia in April 2021. The patient was treated with corticosteroids, tacrolimus, and cyclophosphamide pulse therapy for interstitial pneumonia complicated by MDA5 antibody-positive dermatitis, which improved the symptoms and interstitial pneumonia. Eight months after the start of treatment, a new interstitial shadow appeared that worsened. Therefore, three additional courses of cyclophosphamide pulse therapy were administered; however, the respiratory symptoms and interstitial shadows did not improve. Respiratory failure progressed, and 14 months after treatment initiation, bronchoscopy revealed turbid alveolar lavage fluid, numerous foamy macrophages, and numerous periodic acid-Schiff-positive unstructured materials. Blood test results revealed high anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) antibody levels, leading to a diagnosis of APAP. The patient underwent whole-lung lavage, and the respiratory disturbance promptly improved. Anti-GM-CSF antibodies were measured from the cryopreserved serum samples collected at the time of diagnosis of anti-MDA5 antibody-positive dermatitis, and 10 months later, both values were significantly higher than normal., Conclusions: This is the first report of anti-MDA5 antibody-positive dermatomyositis complicated by interstitial pneumonia with APAP, which may develop during immunosuppressive therapy and be misdiagnosed as a re-exacerbation of interstitial pneumonia. In anti-MDA5 antibody-positive dermatomyositis, APAP comorbidity may have been overlooked, and early evaluation with bronchoalveolar lavage fluid and anti-GM-CSF antibody measurements should be considered, keeping the development of APAP in mind., (© 2024. The Author(s).)
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- 2024
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11. Prevalence and risk factors for medication-refractory reflux esophagitis in patients with systemic sclerosis in Japan.
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Kuribayashi S, Nakamura F, Motegi SI, Hara K, Hosaka H, Sekiguchi A, Ishikawa M, Endo Y, Harada T, Sorimachi H, Obokata M, Uchida M, Yamaguchi K, and Uraoka T
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- Humans, Japan epidemiology, Prevalence, Retrospective Studies, Risk Factors, Proton Pump Inhibitors, Manometry, Esophagitis, Peptic drug therapy, Esophagitis, Peptic epidemiology, Esophagitis, Peptic etiology, Scleroderma, Systemic complications, Scleroderma, Systemic drug therapy, Pyrroles, Sulfonamides
- Abstract
Backgrounds: Patients with systemic sclerosis (SSc) often have esophageal motility abnormalities and weak esophago-gastric junction (EGJ) barrier function, which causes proton pump inhibitor (PPI)-refractory reflux esophagitis (RE). The aims of this study were to clarify the current management of RE and prevalence and risk factors of medication-refractory RE in patients with SSc in Japan., Methods: A total of 188 consecutive patients with SSc who underwent both esophageal high-resolution manometry (HRM) and esophagogastroduodenoscopy (EGD) were reviewed. The presence of RE and grades of the gastroesophageal flap valve (GEFV) were assessed. Esophageal motility was assessed retrospectively according to the Chicago classification v3.0. When RE was seen on a standard dose of PPI or any dose of vonoprazan (VPZ), it was defined as medication-refractory RE., Results: Approximately 80% of patients received maintenance therapy with acid secretion inhibitors regardless of esophageal motility abnormalities. Approximately 50% of patients received maintenance therapy with PPI, and approximately 30% of patients received VPZ. Medication-refractory RE was observed in 30 patients (16.0%). In multivariable analyses, the number of EGD and absent contractility were significant risk factors for medication-refractory RE. Furthermore, combined absent contractility and GEFV grade III or IV had higher odds ratios than did absent contractility alone., Conclusions: Patients with persistent reflux symptoms and those with absent contractility and GEFV grade III or IV should receive maintenance therapy with strong acid inhibition to prevent medication-refractory RE., (© 2024. Japanese Society of Gastroenterology.)
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- 2024
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12. GWAS for systemic sclerosis identifies six novel susceptibility loci including one in the Fcγ receptor region.
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Ishikawa Y, Tanaka N, Asano Y, Kodera M, Shirai Y, Akahoshi M, Hasegawa M, Matsushita T, Saito K, Motegi SI, Yoshifuji H, Yoshizaki A, Kohmoto T, Takagi K, Oka A, Kanda M, Tanaka Y, Ito Y, Nakano K, Kasamatsu H, Utsunomiya A, Sekiguchi A, Niiro H, Jinnin M, Makino K, Makino T, Ihn H, Yamamoto M, Suzuki C, Takahashi H, Nishida E, Morita A, Yamamoto T, Fujimoto M, Kondo Y, Goto D, Sumida T, Ayuzawa N, Yanagida H, Horita T, Atsumi T, Endo H, Shima Y, Kumanogoh A, Hirata J, Otomo N, Suetsugu H, Koike Y, Tomizuka K, Yoshino S, Liu X, Ito S, Hikino K, Suzuki A, Momozawa Y, Ikegawa S, Tanaka Y, Ishikawa O, Takehara K, Torii T, Sato S, Okada Y, Mimori T, Matsuda F, Matsuda K, Amariuta T, Imoto I, Matsuo K, Kuwana M, Kawaguchi Y, Ohmura K, and Terao C
- Subjects
- Humans, Genome-Wide Association Study, Receptors, IgG genetics, Genetic Risk Score, Polymorphism, Single Nucleotide, Interferon Regulatory Factors genetics, Genetic Loci, Genetic Predisposition to Disease genetics, Scleroderma, Systemic genetics
- Abstract
Here we report the largest Asian genome-wide association study (GWAS) for systemic sclerosis performed to date, based on data from Japanese subjects and comprising of 1428 cases and 112,599 controls. The lead SNP is in the FCGR/FCRL region, which shows a penetrating association in the Asian population, while a complete linkage disequilibrium SNP, rs10917688, is found in a cis-regulatory element for IRF8. IRF8 is also a significant locus in European GWAS for systemic sclerosis, but rs10917688 only shows an association in the presence of the risk allele of IRF8 in the Japanese population. Further analysis shows that rs10917688 is marked with H3K4me1 in primary B cells. A meta-analysis with a European GWAS detects 30 additional significant loci. Polygenic risk scores constructed with the effect sizes of the meta-analysis suggest the potential portability of genetic associations beyond populations. Prioritizing the top 5% of SNPs of IRF8 binding sites in B cells improves the fitting of the polygenic risk scores, underscoring the roles of B cells and IRF8 in the development of systemic sclerosis. The results also suggest that systemic sclerosis shares a common genetic architecture across populations., (© 2024. The Author(s).)
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- 2024
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13. Keratinocyte-Specific SOX2 Overexpression Suppressed Pressure Ulcer Formation after Cutaneous Ischemia-Reperfusion Injury via Enhancement of Amphiregulin Production.
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Inoue Y, Uchiyama A, Amalia SN, Ishikawa M, Kosaka K, Sekiguchi A, Ogino S, Yokoyama Y, Torii R, Hosoi M, Akai R, Iwawaki T, Morasso MI, and Motegi SI
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- Animals, Mice, Amphiregulin genetics, Amphiregulin metabolism, Apoptosis, Keratinocytes metabolism, Skin metabolism, Pressure Ulcer, Reperfusion Injury complications, Reperfusion Injury genetics, Reperfusion Injury metabolism
- Abstract
Ischemia-reperfusion (I/R) injury is a key player in the pathogeneses of pressure ulcer formation. Our previous work demonstrated that inducing the transcription factor SOX2 promotes cutaneous wound healing through EGFR signaling pathway enhancement. However, its protective effect on cutaneous I/R injury was not well-characterized. We aimed to assess the role of SOX2 in cutaneous I/R injury and the tissue-protective effect of SOX2 induction in keratinocytes (KCs) in cutaneous I/R injury. SOX2 was transiently expressed in KCs after cutaneous I/R injury. Ulcer formation was significantly suppressed in KC-specific SOX2-overexpressing mice. SOX2 in skin KCs significantly suppressed the infiltrating inflammatory cells, apoptotic cells, vascular damage, and hypoxic areas in cutaneous I/R injury. Oxidative stress-induced mRNA levels of inflammatory cytokine expression were suppressed, and antioxidant stress factors and amphiregulin were elevated by SOX2 induction in skin KCs. Recombinant amphiregulin administration suppressed pressure ulcer development after cutaneous I/R injury in mice and suppressed oxidative stress-induced ROS production and apoptosis in vitro. These findings support that SOX2 in KCs might regulate cutaneous I/R injury through amphiregulin production, resulting in oxidative stress suppression. Recombinant amphiregulin can be a potential therapeutic agent for cutaneous I/R injury., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2024
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14. TRPV4 Regulates the Development of Psoriasis by Controlling Adenosine Triphosphate Expression in Keratinocytes and the Neuroimmune System.
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Amalia SN, Baral H, Fujiwara C, Uchiyama A, Inoue Y, Yamazaki S, Ishikawa M, Kosaka K, Sekiguchi A, Yokoyama Y, Ogino S, Torii R, Hosoi M, Shibasaki K, and Motegi SI
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- Humans, Animals, Mice, Imiquimod pharmacology, TRPV Cation Channels genetics, TRPV Cation Channels metabolism, Adenosine Triphosphate metabolism, Mice, Knockout, Keratinocytes metabolism, Skin metabolism, Disease Models, Animal, Mice, Inbred BALB C, Psoriasis chemically induced, Psoriasis genetics, Psoriasis drug therapy, Dermatitis pathology, Neuropeptides metabolism
- Abstract
TRPV4 is a calcium ion channel that is widely expressed in various cells. It is also involved in physiological and pathological processes. However, the role of TRPV4 in psoriasis remains unknown. We aimed to investigate the role of TRPV4 in psoriasis using human psoriasis skin samples and an imiquimod-induced psoriasis-like mouse model. Keratinocytes in human psoriasis skin had high TRPV4 expression. Trpv4-knockout mice had less severe dermatitis than wild-type mice in the imiquimod-induced mouse model. Knockout mice had significantly reduced epidermal thickness and a low number of infiltrated CD3
+ T cells and CD68+ macrophages on the basis of histopathological studies and decreased mRNA expression of Il17a, Il17f, and Il23, as detected through qPCR. Furthermore, knockout mice had a significantly low expression of neuropeptides and the neuron marker PGP9.5. Adenosine triphosphate release was significantly suppressed by TRPV4 knockdown in both human and mouse keratinocytes in vitro. Finally, treatment with TRPV4 antagonist was significantly effective in preventing the progression of psoriasis-like dermatitis. In conclusion, TRPV4 mediates the expression of keratinocyte-derived adenosine triphosphate and increases the secretion of neuropeptides, resulting in the activation and amplification of IL-23/Th17 responses. Hence, TRPV4 can serve as a novel therapeutic target in psoriasis., (Copyright © 2023. Published by Elsevier Inc.)- Published
- 2023
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15. Transient receptor potential vanilloid 4 promotes cutaneous wound healing by regulating keratinocytes and fibroblasts migration and collagen production in fibroblasts in a mouse model.
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Taivanbat B, Yamazaki S, Nasanbat B, Uchiyama A, Amalia SN, Nasan-Ochir M, Inoue Y, Ishikawa M, Kosaka K, Sekiguchi A, Ogino S, Yokoyama Y, Torii R, Hosoi M, Shibasaki K, and Motegi SI
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- Animals, Mice, Cell Movement genetics, Cell Movement physiology, Collagen metabolism, Disease Models, Animal, Fibroblasts metabolism, Keratinocytes metabolism, RNA, Messenger metabolism, Skin pathology, TRPV Cation Channels genetics, TRPV Cation Channels metabolism, Wound Healing genetics, Wound Healing physiology
- Abstract
Background: Transient receptor potential vanilloid 4 (TRPV4), a cation ion channel, is expressed in different cells, and it regulates the development of different diseases. We recently found a high TRPV4 expression in the wounded skin area. However, the role of TRPV4 in cutaneous wound healing is unknown., Objective: To investigate the role of TRPV4 in cutaneous wound healing in a mouse model., Methods: Skin wound healing experiment and histopathological studies were performed between WT and TRPV4 KO mice. The effect of TRPV4 antagonist and agonist on cell migration, proliferation, and differentiation were examined in vitro., Results: TRPV4 expression was enhanced in wounded area in the skin. TRPV4 KO mice had impaired cutaneous wound healing compared with the WT mice. Further, they had significantly suppressed re-epithelialization and formation of granulation tissue, amount of collagen deposition, and number of α-SMA-positive myofibroblasts in skin wounds. qPCR revealed that the KO mice had decreased mRNA expression of COL1A1 and ACTA2 in skin wounds. In vitro, treatment with selective TRPV4 antagonist suppressed migrating capacity, scratch stimulation enhanced the expression of phospho-ERK in keratinocytes, and TGF-β stimulation enhanced the mRNA expression of COL1A1 and ACTA2 in fibroblasts. Selective TRPV4 agonist suppressed cell migration in keratinocytes, and did not enhance proliferation and migration, but promoted differentiation in fibroblasts., Conclusion: TRPV4 mediates keratinocytes and fibroblasts migration and increases collagen deposition in the wound area, thereby promoting cutaneous wound healing., Competing Interests: Conflict of Interest The authors have no conflicts of interest to declare., (Copyright © 2023 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.)
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- 2023
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16. Skin surface material for detecting human papillomavirus infection of skin warts.
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Kuriyama Y, Kosaka M, Kaneko A, Nishioka H, Anzawa K, Hattori T, Igarashi N, Tamura M, Motegi SI, and Shimizu A
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- Humans, Aged, Human Papillomavirus Viruses, DNA, Viral genetics, Papillomaviridae genetics, Papillomavirus Infections diagnosis, Warts diagnosis, Skin Neoplasms, Foot Diseases
- Abstract
Warts, caused by human papillomavirus (HPV) infection, have various clinical presentations, making them difficult to differentiate from clavus, callus, and sometimes, squamous cell carcinoma. Although skin biopsies are the gold standard, a less-invasive method of examining these lesions is desired. Ninety patients with warts and related diseases, such as clavus and callus, were recruited to explore new differentiation methods using the surface of the warts. DNA was extracted from three types of specimens in each case: surface swab, shaved hyperkeratotic scale, and post-shaved surface swab. Total DNA was successfully extracted from these three specimens and was sufficient for subsequent HPV DNA detection. We analyzed samples for the HPV type and HPV viral load using polymerase chain reaction (PCR). Fifty-five cases were PCR-positive, and HPV1a, 2a, 4, 27, 57, and 65 were detected. The amount of HPV1a DNA produced was significantly greater than that of other HPV types. Regarding the correlation between the clinical diagnosis and HPV detection, the positive agreement rate was 90.9%, the negative agreement rate was 40.0%, and the overall agreement rate was 71.1%. Ten of the 21 cases clinically diagnosed as plantar warts were PCR-negative, especially in elderly patients. This suggests that it is difficult to distinguish plantar warts from clavus and callus in clinical practice. Although the amount of HPV DNA in the removed keratinization scale was highest for all HPV types, HPV detection by swabbing before and after shaving is also useful for follow-up as well as for differential diagnosis., (© 2023 Japanese Dermatological Association.)
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- 2023
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17. Two cases of granular C3 dermatosis with IgA antitransglutaminase 2 antibodies showing linear dermatitis-like clinical features.
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Kuriyama Y, Endo Y, Oka A, Ishii N, Yasuda M, and Motegi SI
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- Humans, Immunoglobulin A, Skin Diseases, Vesiculobullous, Dermatitis
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- 2023
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18. Epidermodysplasia verruciformis with squamous cell carcinomas and the identification of a novel TMC8 mutation.
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Kuriyama Y, Yasuda M, Saito S, Nakano H, Shimizu A, Tamura A, and Motegi SI
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- Humans, Membrane Proteins genetics, Mutation, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell genetics, Epidermodysplasia Verruciformis diagnosis, Epidermodysplasia Verruciformis genetics, Epidermodysplasia Verruciformis pathology, Skin Neoplasms diagnosis, Skin Neoplasms genetics, Skin Neoplasms pathology
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- 2023
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19. Kaempferol therapy improved MC903 induced-atopic dermatitis in a mouse by suppressing TSLP, oxidative stress, and type 2 inflammation.
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Nasanbat B, Uchiyama A, Amalia SN, Inoue Y, Yokoyama Y, Ogino S, Torii R, Hosoi M, and Motegi SI
- Subjects
- Mice, Animals, Filaggrin Proteins, Interleukin-13 metabolism, Interleukin-4 metabolism, Kaempferols pharmacology, Kaempferols therapeutic use, Cytokines metabolism, Skin pathology, Inflammation metabolism, Oxidative Stress, Dermatitis, Atopic chemically induced, Dermatitis, Atopic drug therapy
- Abstract
Background: Atopic dermatitis is a common skin disease caused by genetic susceptibility, environmental factors, immune response, and skin barrier dysfunction. Kaempferol is a natural flavonoid widely found in tea, vegetables, and fruits and has been reported to have excellent anti-inflammation activity. However, the therapeutic effect of kaempferol on atopic dermatitis is unclear., Objective: This study aimed to elucidate the effect of kaempferol on skin inflammation in atopic dermatitis., Methods: The suppressive effect of kaempferol administration on skin inflammation was examined using MC903-induced atopic dermatitis-like skin inflammation mouse model. Quantification of skin dermatitis and transepidermal water loss was performed. A histopathological study was performed to examine thymic stromal lymphopoietin expression, cornified envelope proteins such as filaggrin, loricrin, and involucrin, and the numbers of infiltrating inflammatory cells, including lymphocytes, macrophages, and mast cells in the dermatitis area. The expressions of IL-4 and IL-13 were investigated by qPCR and flow cytometry analysis using skin tissues. The expression of HO-1 was investigated by western blot and qPCR., Results: Kaempferol therapy significantly suppressed MC903-induced dermatitis, TEWL, TSLP, and HO-1 expression, and infiltration of inflammatory cells. Kaempferol therapy improved the decreased expressions of filaggrin, loricrin, and involucrin in MC903-induced dermatitis skin site. The expressions of IL-4, and IL-13 were partially decreased in kaempferol-treated mice., Conclusion: Kaempferol might improve MC903-induced dermatitis via suppression of type 2 inflammation and improvement of barrier dysfunction by inhibition of TSLP expression and oxidative stress. Kaempferol might have the potential to be a new treatment for atopic dermatitis., Competing Interests: Conflict of Interest The authors have no conflicts of interest to declare., (Copyright © 2023 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
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20. Real-world effectiveness and safety of bosentan in Japanese patients with systemic sclerosis: A single-center retrospective study.
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Ishikawa M, Endo Y, Yamazaki S, Sekiguchi A, Uchiyama A, and Motegi SI
- Subjects
- Humans, Bosentan adverse effects, Bosentan therapeutic use, East Asian People, Retrospective Studies, Sulfonamides adverse effects, Treatment Outcome, Scleroderma, Systemic complications, Scleroderma, Systemic drug therapy, Skin Ulcer drug therapy, Skin Ulcer etiology, Skin Ulcer prevention & control
- Abstract
Patients with systemic sclerosis (SSc) develop various vascular disorders, including digital ulcers (DUs), which are sometimes intractable. Bosentan is a dual endothelin receptor antagonist expected to suppress the development of new DUs. The objective of this study was to analyze retrospectively Japanese SSc patients treated with bosentan and investigate its efficacy and safety. We analyzed 40 patients who visited our department from 2009 to 2022 and were treated with bosentan. Of the 25 patients who were able to continue bosentan, 64% (16 patients) were cured by 16 weeks . New DUs occurred in 5.9% (2/34) of patients and the number of new DUs per person was 0.1. Adverse events occurred in 45% (18/40), and hepatic dysfunction was occurred most frequently at 32.5% (13/40). In univariate analysis, hepatic dysfunction was significantly high in patients with low modified Rodnan total skin thickness score. Antimitochondria-antibody-positive patients were more likely to develop liver dysfunction. Hepatic dysfunction was improved without the reduction or discontinuation, dose reduction, discontinuation, or concomitant use of ursodeoxycholic acid. These results suggest that bosentan can be selected as an additional treatment for DU, which is difficult to treat with existing therapies, while carefully monitoring hepatic function., (© 2023 Japanese Dermatological Association.)
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- 2023
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21. Case report: further delineation of AEBP1 -related Ehlers-Danlos Syndrome (classical-like EDS type 2) in an additional patient and comprehensive clinical and molecular review of the literature.
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Yamaguchi T, Hayashi S, Nagai S, Uchiyama A, Motegi SI, Fujikawa T, Takiguchi Y, and Kosho T
- Abstract
The Ehlers-Danlos Syndromes (EDS), a group of hereditary connective tissue disorders, were classified into 13 subtypes in the 2017 International Classification. Recently, a new subtype of EDS called classical-like EDS type 2 (clEDS2), which is caused by biallelic variants in the adipocyte enhancer binding protein 1 ( AEBP1 ) gene, was identified. We describe the 11th patient (9th family) with clEDS2, who was complicated by a critical vascular event (superior mesenteric artery aneurysm and rupture). A next-generation sequencing panel-based analysis revealed compound heterozygous variants in AEBP1 : NM_001129.5:c.[2296G>T]; [2383dup], p.[(Glu766*)]; [(Glu795Glyfs*3)]. Light microscopic analyses showed increased interfibrillar spaces in the reticular dermis, a disorganized arrangement of collagen fibers, and decreased collagen content. An electron microscopic analysis showed the presence of collagen fibrils with irregular contours (flower-like appearance) and small collagen fibrils. A biochemical analysis showed reduced secretion of type I and type III procollagen. Clinical and molecular features of the current patient and all previously reported patients were reviewed comprehensively. Manifestations noted in most cases (>80%) included skin features (hyperextensibility, atrophic scars, easy bruising, excessive skin/skin folding, delayed wound healing, translucency, piezogenic papules), skeletal features (generalized joint hypermobility, dislocations/subluxations, pes planus), dental abnormalities, and neuromuscular abnormalities. Critical complications, each occurring in a single case, included superior mesenteric artery multiple aneurysm and rupture, aortic root dilation requiring surgery, and bowel rupture. Most AEBP1 variants were predicted or experimentally confirmed to lead to nonsense-mediated mRNA decay, whereas one variant resulted in a protein that was retained intracellularly and not secreted. Clinical, molecular, pathological, and biochemical features of the current patient, as well as a review of all previously reported patients, suggest the importance of the aortic carboxypeptidase-like protein encoded by AEBP1 in collagen fibrillogenesis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Yamaguchi, Hayashi, Nagai, Uchiyama, Motegi, Fujikawa, Takiguchi and Kosho.)
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- 2023
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22. Multiple keratoacanthomas induced by treatment with dasatinib and bosutinib for chronic myeloid leukemia: A case report and literature review.
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Kosaka K, Yasuda M, Kim J, Saito S, Uehara A, Handa H, and Motegi SI
- Subjects
- Humans, Dasatinib adverse effects, Chronic Disease, Keratoacanthoma, Leukemia, Myelogenous, Chronic, BCR-ABL Positive drug therapy
- Published
- 2023
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23. Renal dysfunction, malignant neoplasms, atherosclerotic cardiovascular diseases, and sarcopenia as key outcomes observed in a three-year follow-up study using the Werner Syndrome Registry.
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Maeda Y, Koshizaka M, Shoji M, Kaneko H, Kato H, Maezawa Y, Kawashima J, Yoshinaga K, Ishikawa M, Sekiguchi A, Motegi SI, Nakagami H, Yamada Y, Tsukamoto S, Taniguchi A, Sugimoto K, Takami Y, Shoda Y, Hashimoto K, Yoshimura T, Kogure A, Suzuki D, Okubo N, Yoshida T, Watanabe K, Kuzuya M, Takemoto M, Oshima J, and Yokote K
- Subjects
- Humans, Kidney, Follow-Up Studies, Cross-Sectional Studies, Creatinine, Werner Syndrome complications, Werner Syndrome epidemiology, Sarcopenia, Cardiovascular Diseases, Kidney Diseases, Neoplasms complications, Neoplasms epidemiology
- Abstract
Werner syndrome is an adult-onset progeria syndrome that results in various complications. This study aimed to clarify the profile and secular variation of the disease. Fifty-one patients were enrolled and registered in the Werner Syndrome Registry. Their data were collected annually following registration. A cross-sectional analysis at registration and a longitudinal analysis between the baseline and each subsequent year was performed. Pearson's chi-squared and Wilcoxon signed-rank tests were used. Malignant neoplasms were observed from the fifth decade of life (mean onset: 49.7 years) and were observed in approximately 30% of patients during the 3-year survey period. Regarding renal function, the mean estimated glomerular filtration rate calculated from serum creatinine (eGFRcre) and eGFRcys, which were calculated from cystatin C in the first year, were 98.3 and 83.2 mL/min/1.73 m
2 , respectively, and differed depending on the index used. In longitudinal analysis, the average eGFRcre for the first and fourth years was 74.8 and 63.4 mL/min/1.73 m2 , showing a rapid decline. Secular changes in Werner syndrome in multiple patients were identified. The prevalence of malignant neoplasms is high, and renal function may decline rapidly. It is, therefore, necessary to carry out active and detailed examinations and pay attention to the type and dose of the drugs used.- Published
- 2023
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24. Mycosis fungoides responded to dasatinib for acute lymphoblastic leukemia.
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Yasuda M, Shang C, Yamanaka M, Amano H, Tsukamoto N, Ishikawa O, and Motegi SI
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- Humans, Dasatinib therapeutic use, Mycosis Fungoides drug therapy, Skin Neoplasms diagnosis, Skin Neoplasms drug therapy, Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy
- Published
- 2023
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25. Pemphigus vegetans with antibodies against desmoglein 1 and desmocollin 1-3: a case report and literature review.
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Kosaka K, Endo Y, Toki S, Oka A, Uehara A, Ishikawa O, Koga H, Ishii N, and Motegi SI
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- Humans, Desmocollins, Desmoglein 1, Desmoglein 3, Antibodies, Autoantibodies, Pemphigus
- Published
- 2022
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26. Nationwide time-series surveys of pressure ulcer prevalence in Japan.
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Konya C, Takeuchi Y, Nakagami G, Kitamura A, Morita K, Ishizawa M, Abe Y, Higuchi H, Mizuki T, Motegi SI, Shoju S, Shimada K, Tanaka K, Kawakami S, and Sanada H
- Subjects
- Humans, Japan epidemiology, Pressure Ulcer epidemiology, Pressure Ulcer prevention & control
- Abstract
Objective: The Japanese Society of Pressure Ulcers (JSPU) has two purposes: first, to improve knowledge and skills among health professionals related to preventing and managing pressure ulcers (PUs); and second, to represent those in the field managing PUs, including with government and health authorities. Since 2006, JSPU has conducted fact-finding surveys about every four years to identify PU prevalence in Japan (2006, 2010, 2013 and 2016). Based on the prevalence identified by these surveys, an attempt was made to validate the achievements of JSPU's activities., Method: Information from one-day surveys of hospitals, long-term care health facilities, long-term care welfare facilities, and home visit nursing care stations was analysed. We used generalised estimating equations to estimate the proportions of PUs and their 95% confidence intervals (CIs) for each survey., Results: A total of 662,419 patients in 2631 facilities participated in the surveys. The estimated proportions for all facilities (95% CI) in chronological order, from the first to the fourth survey, were: 2.67% (2.52-2.83); 2.61% (2.43-2.80); 1.99% (1.83-2.17); and 1.79% (1.65-1.94), respectively. In all facility types, the proportion of PUs was lower in the fourth survey than the first survey., Conclusion: The proportion of PUs showed a decreasing trend and was low according to global standards, demonstrating the efficacy of JSPU's activities.
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- 2022
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27. Possible suppressive effects of tranilast on NLRP3 inflammasome activation in necrobiosis lipoidica.
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Uchiyama A, Kosaka K, Okada K, and Motegi SI
- Subjects
- Humans, NLR Family, Pyrin Domain-Containing 3 Protein, Inflammasomes, ortho-Aminobenzoates pharmacology, ortho-Aminobenzoates therapeutic use, Necrobiosis Lipoidica drug therapy
- Published
- 2022
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28. Real-world effectiveness and safety of upadacitinib in Japanese patients with atopic dermatitis: a two-centre retrospective study
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Kosaka K, Uchiyama A, Ishikawa M, Watanabe G, and Motegi SI
- Subjects
- Humans, East Asian People, Retrospective Studies, Dermatitis, Atopic drug therapy, Heterocyclic Compounds, 3-Ring therapeutic use
- Published
- 2022
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29. The differential expression of long interspersed nuclear elements-1 as a marker for hypomethylation in Merkel cell carcinoma.
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Kuriyama Y, Shimizu A, Kim J, Yasuda M, and Motegi SI
- Subjects
- Biomarkers, Humans, Carcinoma, Merkel Cell genetics, Carcinoma, Merkel Cell pathology, Merkel cell polyomavirus genetics, Polyomavirus Infections, Skin Neoplasms pathology, Tumor Virus Infections
- Abstract
The protein long interspersed nuclear elements-1 (LINE-1) serves as a useful surrogate marker of global methylation but little is known for Merkel cell carcinoma. LINE-1 expression was found only in Merkel cell polyomavirus (MCPyV)-positive Merkel cell carcinomas but not in MCPyV-negative Merkel cell carcinomas, suggesting that epigenetic dysregulation may be associated with MCPyV expression., (© 2022 British Association of Dermatologists.)
- Published
- 2022
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30. Capillaroscopy in Pigmented Basal Cell Carcinoma Smaller than 3-mm Diameter: A Report of Four Lesions.
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Saito S, Kuriyama Y, Uchiyama A, Yasuda M, and Motegi SI
- Subjects
- Dermoscopy, Diagnosis, Differential, Humans, Microscopic Angioscopy, Carcinoma, Basal Cell diagnostic imaging, Carcinoma, Basal Cell surgery, Skin Neoplasms diagnostic imaging, Skin Neoplasms surgery
- Published
- 2022
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31. Case of recurrent cutaneous eosinophilic vasculitis with subcutaneous nodules in the early stage of the disease.
- Author
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Uehara A, Endo Y, Kosaka K, Oka A, Ishikawa O, and Motegi SI
- Subjects
- Humans, Churg-Strauss Syndrome, Skin Diseases, Vascular, Skin Neoplasms
- Published
- 2022
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32. An autopsy case of granulomatous amebic encephalitis caused by Balamuthia mandrillaris involving prior amebic dermatitis.
- Author
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Maehara T, Mizuno T, Tokoro M, Hara T, Tomita Y, Makioka K, Motegi SI, Yamazaki A, Matsumura N, Nobusawa S, and Yokoo H
- Subjects
- Aged, 80 and over, Autopsy, Brain pathology, Granuloma pathology, Humans, Male, Retrospective Studies, Amebiasis diagnosis, Amoeba, Balamuthia mandrillaris, Dermatitis pathology, Encephalitis, Infectious Encephalitis pathology
- Abstract
An 82-year-old man, who was healthy and had worked as a farmer, experienced worsening neurological symptoms over a seven-month period, which eventually caused his death. Multiple fluctuating brain lesions were detected radiographically. Clinically, sarcoidosis was ranked high among the differential diagnoses because of the presence of skin lesions showing granulomatous inflammation, confirmed by biopsy. The patient's cerebrospinal fluid was also examined, but no definitive diagnosis was made while he was alive. An autopsy revealed multiple granulomatous amebic encephalitis lesions in the brain. Genetic and immunohistochemical analyses identified Balamuthia (B.) mandrillaris, a free-living ameba, which resides in soil and fresh water, as the causative organism. A retrospective examination revealed B. mandrillaris in the biopsied skin as well as cerebrospinal fluid, strongly suggesting that the ameba had spread into the brain percutaneously. Few studies have detailed the cutaneous pathology of B. mandrillaris infections. In general, granulomatous amebic encephalitis is extremely difficult to diagnose without autopsy, but the present case provides a clue that could allow similar cases to be diagnosed earlier; that is, the presence of skin lesions., (© 2022 The Authors. Neuropathology published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Neuropathology.)
- Published
- 2022
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33. Possible suppressive effects of baricitinib on serum IL-22 levels in atopic dermatitis.
- Author
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Uchiyama A, Fujiwara C, Inoue Y, Ishikawa M, and Motegi SI
- Subjects
- Humans, Interleukins, Purines, Pyrazoles, Sulfonamides, Interleukin-22, Azetidines pharmacology, Azetidines therapeutic use, Dermatitis, Atopic drug therapy
- Abstract
Competing Interests: Declaration of Competing Interest The authors have no conflict of interest to declare.
- Published
- 2022
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34. Spontaneous pneumomediastinum developed after steroid pulse therapy in diffuse cutaneous systemic sclerosis patient: A case report.
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Endo Y, Kim J, Uchiyama A, Yasuda M, Hara K, and Motegi SI
- Subjects
- Humans, Steroids adverse effects, Mediastinal Emphysema chemically induced, Mediastinal Emphysema diagnostic imaging, Scleroderma, Diffuse complications, Scleroderma, Diffuse drug therapy
- Published
- 2022
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35. Quantitative CT analysis of interstitial pneumonia in anti-melanoma differentiation-associated gene 5 antibody-positive dermatomyositis: a single center, retrospective study.
- Author
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Yamaguchi K, Nakajima T, Yamaguchi A, Itai M, Onuki Y, Shin Y, Uno S, Muto S, Kouno S, Yatomi M, Aoki-Saito H, Hara K, Endo Y, Motegi SI, Muro Y, Nakasatomi M, Sakairi T, Hiromura K, Katsumata N, Hirasawa H, Tsushima Y, and Maeno T
- Subjects
- Autoantibodies, Humans, Interferon-Induced Helicase, IFIH1, Prognosis, Retrospective Studies, Tomography, X-Ray Computed, Dermatomyositis complications, Dermatomyositis diagnostic imaging, Lung Diseases, Interstitial complications
- Abstract
Introduction: This study aimed to assess the utility of quantitative high-resolution computed tomography (HRCT) for determining the clinical course of anti-melanoma differentiation-associated gene 5 antibody-positive dermatomyositis-associated interstitial lung disease (MDA5
+ ILD)., Method: This study retrospectively analyzed the data of 34 patients with MDA5+ ILD to determine the association between the clinical findings and extent of ILD via quantitative CT analysis at baseline and short-term follow-up. Quantified HRCT scores were evaluated as the lung severity score (LSS), percentage of opacity, and percentage of high opacity., Results: Thirty-four patients underwent follow-up CT scans 35 (range: 14-78) days after diagnosis. Patients who died of rapidly progressive ILD had higher LSS (p < 0.01), percentage of opacity (p < 0.01), percentage of high opacity (p = 0.01), total ground-glass opacity score (p = 0.01), serum C-reactive protein (CRP) (p = 0.03), and alveolar-arterial oxygen difference (Aa-DO2) (p = 0.01) at follow-up than those who survived. Quantified HRCT scores correlated with serum CRP and Aa-DO2 levels at follow-up. LSS at follow-up (AUC = 0.844, p < 0.01) was the best predictor of death in MDA5+ ILD patients. Patients with an LSS of > 6.5 at follow-up had higher mortality than those with an LSS of ≤ 6.5, especially when receiving triple therapy. In multivariate analysis, an LSS of > 6.5 at follow-up was significantly associated with a poor outcome., Conclusions: Quantitative CT analysis of MDA5+ ILD is useful for the objective assessment of respiratory status and disease activity. Short-term HRCT evaluation, particularly LSS, is most important in predicting its clinical course during triple therapy. Key Points • Quantitative CT analysis plays an important role in evaluating the clinical course of anti-melanoma differentiation-associated gene 5 antibody-positive dermatomyositis-associated interstitial lung disease (MDA5+ ILD). • Quantified HRCT scores, particularly lung severity score, at short-term intervals from diagnosis can help to predict prognosis after triple therapy in MDA5+ ILD., (© 2022. International League of Associations for Rheumatology (ILAR).)- Published
- 2022
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36. Real-world effectiveness and safety of baricitinib in Japanese patients with atopic dermatitis: A single-center retrospective study.
- Author
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Uchiyama A, Fujiwara C, Inoue Y, and Motegi SI
- Subjects
- Humans, Japan, Purines, Pyrazoles, Retrospective Studies, Sulfonamides, Treatment Outcome, Azetidines adverse effects, Dermatitis, Atopic drug therapy
- Published
- 2022
- Full Text
- View/download PDF
37. Influence of obesity in interstitial lung disease associated with anti-aminoacyl-tRNA synthetase antibodies.
- Author
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Yamaguchi K, Fukushima Y, Yamaguchi A, Itai M, Shin Y, Uno S, Muto S, Kouno S, Tsurumaki H, Yatomi M, Aoki-Saito H, Hara K, Koga Y, Sunaga N, Endo Y, Motegi SI, Nakasatomi M, Sakairi T, Ikeuchi H, Hiromura K, Hisada T, Tsushima Y, Kuwana M, and Maeno T
- Subjects
- Autoantibodies, Humans, Obesity complications, Retrospective Studies, Amino Acyl-tRNA Synthetases, Lung Diseases, Interstitial complications, Lung Diseases, Interstitial diagnostic imaging
- Abstract
Background: Obesity is a major risk factor for developing various respiratory diseases. Patients with anti-aminoacyl tRNA synthetase (ARS) antibodies often have interstitial lung disease (ILD). The present study was conducted to evaluate the association between obesity and outcomes of anti-ARS antibody-related ILD (ARS-ILD)., Methods: We retrospectively investigated 58 patients with ARS-ILD and compared the clinical characteristics, treatment, and prognoses between obese (body mass index [BMI] ≥25 kg/m
2 ) and nonobese (BMI <25 kg/m2 ) patients. Chest fat was quantified via computed tomography (CT). Thoracic subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) were measured at diagnosis and first relapse of ILD., Results: Sixteen patients were obese. Obese patients had lower percentages of predicted diffusing capacity of the lungs for carbon monoxide and higher high-resolution CT scores and SAT and VAT indexes than did nonobese patients. The ILD relapse rate was higher in obese patients (P < 0.01), especially among those with high SAT indexes (P < 0.01). The SAT and VAT indexes increased significantly from diagnosis until first relapse. Among clinical parameters at first relapse, SAT and VAT indexes were correlated with serum Krebs von den Lungen-6 levels (r = 0.720, P = 0.008) and total ground-glass attenuation scores (r = 0.620, P = 0.024), respectively., Conclusions: Obesity and high SAT indexes are risk factors for ILD relapse in patients positive for anti-ARS antibodies. Evaluating and quantifying patients' chest fat on CT is important for predicting ILD relapse., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2022
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38. Focal palmoplantar keratoderma in a patient with the KRT6B mutation.
- Author
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Kuriyama Y, Shimizu A, Kanai S, Yokoyama Y, Yasuda M, Endo Y, Kawabata-Iwakawa R, and Motegi SI
- Subjects
- Aged, 80 and over, Humans, Male, Mutation, Pedigree, Keratoderma, Palmoplantar diagnosis, Keratoderma, Palmoplantar genetics, Keratoderma, Palmoplantar, Diffuse, Papilloma
- Published
- 2022
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39. Anti-polymyositis/Scl antibody-positive overlap syndrome of diffuse cutaneous systemic sclerosis, dermatomyositis, systemic lupus erythematosus, and antiphospholipid syndrome.
- Author
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Saito S, Endo Y, Nishio M, Uchiyama A, Uehara A, Toki S, Yasuda M, Ishikawa O, Muro Y, and Motegi SI
- Subjects
- Adult, Autoantibodies, Humans, Male, Antiphospholipid Syndrome complications, Antiphospholipid Syndrome diagnosis, Antiphospholipid Syndrome drug therapy, Dermatomyositis complications, Dermatomyositis diagnosis, Dermatomyositis drug therapy, Lupus Erythematosus, Systemic, Polymyositis, Scleroderma, Diffuse, Scleroderma, Systemic complications, Scleroderma, Systemic diagnosis, Scleroderma, Systemic drug therapy
- Abstract
A 37-year-old Japanese man with a 3-year history of diffuse cutaneous systemic sclerosis was admitted to our hospital with high fever, arthralgia, myalgia, and muscle weakness. A physical examination revealed facial erythema, Gottron's sign, and mechanic's hands in addition to skin sclerosis. Laboratory data revealed significantly elevated levels of creatine kinase and decreased complement. Anti-RNP, anti-Smith, anti-DNA, anti-β
2 -glycoprotein 1, anti-polymyositis (PM)/Scl75, and anti-PM/Scl100 antibodies were detected. He also had urinary protein, interstitial lung disease, pericarditis, multifocal cerebral infarctions, and leukoencephalopathy. Thus, a diagnosis of overlap syndrome of diffuse cutaneous systemic sclerosis, dermatomyositis, and systemic lupus erythematosus with antiphospholipid syndrome was made. Because of the intractable course, he was treated with multiple immunosuppressive and immunomodulatory drugs, including three rounds of 1000 mg methylprednisolone pulse therapy. This is the first case report of anti-PM/Scl antibody-positive overlap syndrome of three major connective tissue diseases., (© 2021 Japanese Dermatological Association.)- Published
- 2022
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40. Tumor suppressive effect of anti-PD-1 antibody against angiosarcoma in a mouse model.
- Author
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Sekiguchi A, Ishikawa M, Fujiwara C, Inoue Y, Yamazaki S, Uchiyama A, and Motegi SI
- Subjects
- Animals, Cell Line, Tumor, Disease Models, Animal, Mice, Programmed Cell Death 1 Receptor, Hemangiosarcoma drug therapy
- Published
- 2022
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41. Rothmund-Thomson syndrome investigated by two nationwide surveys in Japan.
- Author
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Kaneko H, Takemoto M, Murakami H, Ihara K, Kosaki R, Motegi SI, Taniguchi A, Matsuo M, Yamazaki N, Nishigori C, Takita J, Koshizaka M, Maezawa Y, and Yokote K
- Subjects
- Humans, Japan epidemiology, Mutation, Quality of Life, Surveys and Questionnaires, Rothmund-Thomson Syndrome diagnosis, Rothmund-Thomson Syndrome epidemiology, Rothmund-Thomson Syndrome genetics
- Abstract
Background: Rothmund-Thomson syndrome (RTS) is an autosomal recessive genetic disorder characterized by poikiloderma of the face, small stature, sparse scalp hair, juvenile cataract, radial aplasia, and predisposition to cancers. Due to the rarity of RTS, the situation of patients with RTS in Japan has not been elucidated., Methods: In 2010 and 2020, following the results of a primary questionnaire survey, a secondary questionnaire survey on RTS was conducted nationwide to investigate the number of RTS cases and their associated skin lesions, bone lesions, other clinical features, and quality of life in Japan., Results: In 2010 and 2020, 10 and eight patients with RTS were recruited, respectively. Skin lesions such as poikiloderma, erythema, pigmentation, and abnormal scalp hair were observed in almost all cases. Bone lesions were observed in four cases in the 2010 and 2020 surveys, respectively. Two cases had mutations in the RECQL4 gene in the 2020 survey., Conclusions: Two nationwide surveys have shown the actual situation of patients with RTS in Japan. Cutaneous and bone manifestations are important for the diagnosis of RTS. However, many patients have no RECQL4 mutations. The novel causative gene of RTS should be further elucidated., (© 2022 Japan Pediatric Society.)
- Published
- 2022
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42. Coordination of retrotransposons and type I interferon with distinct interferon pathways in dermatomyositis, systemic lupus erythematosus and autoimmune blistering disease.
- Author
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Kuriyama Y, Shimizu A, Kanai S, Oikawa D, Motegi SI, Tokunaga F, and Ishikawa O
- Subjects
- Adaptive Immunity, Adult, Aged, Autoimmune Diseases genetics, Cell Line, DNA Methylation, Dermatomyositis genetics, Female, Gene Expression Profiling, Gene Expression Regulation, Humans, Long Interspersed Nucleotide Elements, Lupus Erythematosus, Systemic genetics, Male, Middle Aged, Polymerase Chain Reaction, Promoter Regions, Genetic, RNA, Messenger metabolism, Rheumatic Diseases metabolism, Signal Transduction, Autoimmune Diseases immunology, Dermatomyositis immunology, Interferon Type I genetics, Lupus Erythematosus, Systemic immunology, Retroelements
- Abstract
Type I interferon (IFN) plays a crucial role in innate and adaptive immunity, and aberrant IFN responses are involved in systemic autoimmune diseases, such as systemic lupus erythematosus (SLE) and dermatomyositis (DM). Type I IFNs can be induced by transcribed retrotransposons. The regulation of retrotransposons and type I IFN and the downstream IFN pathways in SLE, DM, and autoimmune blistering disease (AIBD) were investigated. The gene expression levels of retrotransposons, including LINE-1, type I-III IFNs, and IFN-stimulated genes (ISGs) in peripheral blood cells from patients with DM (n = 24), SLE (n = 19), AIBD (n = 14) and healthy controls (HCs, n = 10) were assessed by quantitative polymerase chain reaction. Upregulation of retrotransposons and IFNs was detected in DM patient samples, as is characteristic, compared to HCs; however, ISGs were not uniformly upregulated. In contrast, retrotransposons and IFNs, except for type II IFN, such as IFN-γ, were not upregulated in SLE. In AIBD, only some retrotransposons and type I interferons were upregulated. The DM, SLE, and AIBD samples showed coordinated expression of retrotransposons and type I IFNs and distinct spectra of IFN signaling. A positive correlation between LINE-1 and IFN-β1 was also detected in human cell lines. These factors may participate in the development of these autoimmune diseases., (© 2021. The Author(s).)
- Published
- 2021
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43. Neutrophil elastase in the development of nephrogenic systemic fibrosis (NSF)-like skin lesion in renal failure mouse model.
- Author
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Kartamihardja AAP, Amalia SN, Sekiguchi A, Bhattarai A, Taketomi-Takahashi A, Motegi SI, Koyama H, and Tsushima Y
- Subjects
- Animals, Antigens, CD genetics, Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic genetics, Antigens, Differentiation, Myelomonocytic metabolism, CD3 Complex genetics, CD3 Complex metabolism, Cytokines genetics, Cytokines metabolism, Female, Mice, Skin metabolism, Skin pathology, Transforming Growth Factor beta genetics, Transforming Growth Factor beta metabolism, Contrast Media toxicity, Gadolinium toxicity, Leukocyte Elastase metabolism, Nephrogenic Fibrosing Dermopathy etiology, Renal Insufficiency complications, Skin drug effects
- Abstract
Although neutrophil elastase (NE) may play a role in lung fibrosis and liver fibrosis, NE involvement in the development of nephrogenic systemic fibrosis has been unclear. We investigated the involvement of NE in the development of nephrogenic systemic fibrosis-like skin lesions post-injections of linear gadolinium-based contrast agents in renal failure mouse models. Renal failure mouse models were randomly divided into three groups: control group (saline), gadodiamide group, and gadopentetate group. Each solution was intravenously administered three times per week for three weeks. The mice were observed daily for skin lesions. Quantification of skin lesions, infiltrating inflammatory cells, and profibrotic cytokines in the affected skin was performed by immunostaining and reverse-transcription polymerase chain reaction (RT-PCR). Blood samples were collected from the facial vein to quantify NE enzymatic activity. The 158Gd concentrations in each sample were quantified using inductively coupled plasma mass spectrometry (ICP-MS). In the gadodiamide group, the mRNA expression of fibrotic markers was increased in the skin lesions compared to the control group. In the gadopentetate group, only collagen 1α and TGF-β mRNA expression were higher than in the control group. The expression of CD3+, CD68+, NE cells and the NE activity in the blood serum were significantly higher in the gadodiamide and gadopentetate groups compared to the control group. Gadolinium concentration in the skin of the gadodiamide group was significantly higher than the gadopentetate group, while almost no traces of gadolinium were found in the control group. Although gadopentetate and gadodiamide affected the fibrotic markers in the skin differently, NE may be involved in the development of fibrosis linked to the GBCAs injections in renal failure mouse models., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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44. Antifibrotic effects and mechanisms of mesenchymal stem cell-derived exosomes in a systemic sclerosis mouse model: Possible contribution of miR-196b-5p.
- Author
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Baral H, Uchiyama A, Yokoyama Y, Sekiguchi A, Yamazaki S, Amalia SN, Inoue Y, Ogino S, Torii R, Hosoi M, Matsuzaki T, and Motegi SI
- Subjects
- Animals, Bleomycin administration & dosage, Bleomycin toxicity, Cells, Cultured, Collagen Type I metabolism, Disease Models, Animal, Exosomes metabolism, Female, Fibroblasts metabolism, Fibrosis, Humans, Mice, Receptor, Transforming Growth Factor-beta Type I metabolism, Scleroderma, Systemic chemically induced, Scleroderma, Systemic pathology, Skin cytology, Skin drug effects, Transforming Growth Factor beta metabolism, Exosomes transplantation, Mesenchymal Stem Cells cytology, MicroRNAs metabolism, Scleroderma, Systemic therapy, Skin pathology
- Abstract
Background: Systemic sclerosis (SSc) is a connective tissue disorder characterized by the development of fibrosis in the skin and internal organs. Increasing evidence suggests that mesenchymal stem cells (MSCs) can be used to a treatment for fibrotic diseases. Recent studies have demonstrated that some of the biological effects of MSCs are due to the secretion of exosomes. However, the precise mechanisms underlying MSCs-derived exosomes in skin fibrosis are not well understood., Objective: We aimed to elucidate the effect of MSCs-derived exosomes on skin fibrosis in SSc and the mechanism underlying their inhibitory action on fibrosis., Methods: Exosome was collected from MSCs by ultracentrifugation method. We examined the suppressive effect of MSCs-derived exosome on skin fibrosis in bleomycin-induced SSc mouse model. Skin samples from the injected site were collected for further examination, and micro-RNA analysis of MSCs-derived exosome was performed., Results: Injection of MSCs-derived exosomes significantly inhibited bleomycin-induced dermal fibrosis in mice. MSCs-derived exosomes significantly reduced the amount of collagen and the number of α-SMA
+ myofibroblasts and CD68+ macrophages in lesional skin. They also reduced the expression of type I collagen and TGF-β receptor 1 in fibroblasts in vitro. Moreover, micro-RNA analysis revealed that several microRNAs in MSCs-derived exosomes have antifibrotic potential. We confirmed that overexpression of miR-196b-5p in fibroblasts significantly suppressed collagen type I alpha 2 expression., Conclusion: This study demonstrated that inhibition of collagen type I expression by miR-196b-5p in exosomes might be one of the mechanisms by which MSCs suppress skin fibrosis in an SSc mouse model., Competing Interests: Declaration of Competing Interest The authors have no conflict of interest to declare., (Copyright © 2021 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.)- Published
- 2021
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45. Erythema nodosum-like eruption in coronavirus disease 2019: A case report and literature review of Asian countries.
- Author
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Kuriyama Y, Shimizu A, Oka H, Sato M, Makioka K, Ikota H, Yanagisawa K, Tokue Y, Tsukagoshi H, and Motegi SI
- Subjects
- Asia, Humans, SARS-CoV-2, COVID-19, Erythema Nodosum diagnosis, Erythema Nodosum drug therapy, Exanthema
- Abstract
In the worldwide coronavirus disease 2019 (COVID-19) outbreak, skin manifestations were seen in COVID-19 patients. We report a case in which a COVID-19 patient developed cutaneous lesions that were diagnosed as erythema nodosum-like lesions, which were associated with COVID-19. Nasopharyngeal swab polymerase chain reaction (PCR) confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Histopathologically, extensive inflammation was seen from the epidermis to the fat tissue. An organized thrombus and disrupted inner elastic lamina were seen in an intradermal vessel. These findings suggest septal panniculitis with cutaneous polyarteritis nodosa. The results of PCR using the specimen of skin lesion was negative. The patient took non-steroidal anti-inflammatory drugs and the skin lesion improved in 3 weeks. To characterize the skin eruption, we reviewed previous reports on COVID-19 (confirmed by the detection of SARS-CoV-2 infection) from Asian countries. The type of eruption and timing of its appearance in this case seemed rare. Differences in skin manifestations between Western and Asian countries were noted., (© 2021 Japanese Dermatological Association.)
- Published
- 2021
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46. Impact of muscle volume loss on acute oral mucositis in patients undergoing concurrent chemoradiotherapy after oral cancer resection.
- Author
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Yamaguchi T, Makiguchi T, Nakamura H, Yamatsu Y, Hirai Y, Shoda K, Suzuki K, Kim M, Kurozumi S, Motegi SI, Shirabe K, and Yokoo S
- Subjects
- Chemoradiotherapy adverse effects, Cisplatin, Humans, Muscles, Head and Neck Neoplasms, Mouth Neoplasms, Stomatitis etiology
- Abstract
This study evaluated the association between skeletal muscle mass depletion and severe oral mucositis in patients undergoing concurrent chemoradiotherapy after oral cancer resection. Skeletal muscle mass was evaluated in 60 patients using the skeletal muscle index, which was based on skeletal muscle cross-sectional area (on computed tomography) at the level of the third lumbar vertebra. In accordance with the grading criteria of the Radiation Therapy Oncology Group, patients with a grade ≥3 were defined as having severe oral mucositis. Multivariate logistic regression analysis was used to evaluate independent risk factors for severe oral mucositis. Eleven patients (18.3%) were diagnosed with low skeletal muscle mass. Severe oral mucositis occurred in 17 (28.3%) patients, and the mean skeletal muscle index was 42.8 cm
2 /m2 . A low skeletal muscle mass (hazard ratio 18.1; P=0.001) and a chemotherapy regimen consisting of 5-fluorouracil and cisplatin (versus cisplatin only) (hazard ratio 5.5; P=0.015) were independent risk factors for severe oral mucositis. Future prospective studies are warranted to identify effective pre- and perioperative exercises and nutrition programmes to increase low skeletal muscle mass and reduce the incidence of severe oral mucositis in patients undergoing concurrent chemoradiotherapy after oral cancer resection., (Copyright © 2020 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)- Published
- 2021
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47. Novel mutation in COL7A1 in recessive dystrophic epidermolysis bullosa successfully treated with cultured epidermal autograft transplantation.
- Author
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Kuriyama Y, Shimizu A, Kosaka K, Yasuda M, Shinkuma S, Ishikawa O, and Motegi SI
- Subjects
- Autografts, Genes, Recessive, Humans, Male, Middle Aged, Mutation, Pedigree, Collagen Type VII genetics, Epidermis transplantation, Epidermolysis Bullosa Dystrophica genetics, Epidermolysis Bullosa Dystrophica surgery
- Published
- 2021
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- View/download PDF
48. Clinical course and background of eight patients who discontinued secukinumab after achieving a score of 0 on the psoriasis area and severity index.
- Author
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Yasuda M, Morimoto N, Inoue Y, Ishikawa O, and Motegi SI
- Subjects
- Double-Blind Method, Humans, Severity of Illness Index, Treatment Outcome, Antibodies, Monoclonal, Humanized, Psoriasis diagnosis, Psoriasis drug therapy
- Published
- 2021
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49. Inhibition of skin fibrosis in systemic sclerosis by botulinum toxin B via the suppression of oxidative stress.
- Author
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Baral H, Sekiguchi A, Uchiyama A, Nisaa Amalia S, Yamazaki S, Inoue Y, Yokoyama Y, Ogino S, Torii R, Hosoi M, Akai R, Iwawaki T, Ishikawa O, and Motegi SI
- Subjects
- Animals, Bleomycin, Disease Models, Animal, Fibroblasts pathology, Fibrosis, Mice, Oxidative Stress, Skin pathology, Scleroderma, Systemic pathology, Skin Diseases pathology
- Abstract
Oxidative stress has been reported to play an important role in the pathogenesis of skin fibrosis in systemic sclerosis (SSc). We previously identified that botulinum toxin (BTX) injection suppresses pressure ulcer formation in a cutaneous ischemia-reperfusion injury mouse model by regulation of oxidative stress. However, the therapeutic possibility of BTX administration for preventing skin fibrosis in SSc is unclear. The objective of this study was to investigate the effect of BTX-B on skin fibrosis in a murine model of SSc and determine the underlying mechanism. We found that BTX-B injection significantly reduced dermal thickness and inflammatory cell infiltration in bleomycin-induced skin fibrosis lesion in mice. We also identified that the oxidative stress signal detected through bioluminescence in OKD48 mice after bleomycin injection in the skin was significantly decreased by BTX-B. Additionally, mRNA levels of oxidative stress associated factors (NOX2, HO-1, Trx2) were significantly decreased by BTX-B. Apoptotic cells in the lesional skin of bleomycin-treated mice were significantly reduced by BTX-B. Oxidant-induced intracellular accumulation of reactive oxygen species in SSc fibroblasts was also inhibited by BTX-B. In conclusion, BTX-B might improve bleomycin-induced skin fibrosis via the suppression of oxidative stress and inflammatory cells in the skin. BTX-B injection may have a therapeutic effect on skin fibrosis in SSc., (© 2021 Japanese Dermatological Association.)
- Published
- 2021
- Full Text
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50. Loss of DLX3 tumor suppressive function promotes progression of SCC through EGFR-ERBB2 pathway.
- Author
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Bajpai D, Mehdizadeh S, Uchiyama A, Inoue Y, Sawaya A, Overmiller A, Brooks SR, Hasneen K, Kellett M, Palazzo E, Motegi SI, Yuspa SH, Cataisson C, and Morasso MI
- Subjects
- Aged, Animals, Carcinogens toxicity, Carcinoma, Squamous Cell chemically induced, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell metabolism, Disease Models, Animal, ErbB Receptors genetics, ErbB Receptors metabolism, Female, Homeodomain Proteins metabolism, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Neoplasm Grading, Receptor, ErbB-2 genetics, Signal Transduction, Skin Neoplasms chemically induced, Skin Neoplasms genetics, Skin Neoplasms metabolism, Survival Rate, Tetradecanoylphorbol Acetate toxicity, Transcription Factors metabolism, 9,10-Dimethyl-1,2-benzanthracene toxicity, Carcinoma, Squamous Cell pathology, Homeodomain Proteins genetics, Receptor, ErbB-2 metabolism, Skin Neoplasms pathology, Transcription Factors genetics
- Abstract
Cutaneous squamous cell carcinoma (cSCC) ranks second in the frequency of all skin cancers. The balance between keratinocyte proliferation and differentiation is disrupted in the pathological development of cSCC. DLX3 is a homeobox transcription factor which plays pivotal roles in embryonic development and epidermal homeostasis. To investigate the impact of DLX3 expression on cSCC prognosis, we carried out clinicopathologic analysis of DLX3 expression which showed statistical correlation between tumors of higher pathologic grade and levels of DLX3 protein expression. Further, Kaplan-Meier survival curve analysis demonstrated that low DLX3 expression correlated with poor patient survival. To model the function of Dlx3 in skin tumorigenesis, a two-stage dimethylbenzanthracene (DMBA)/12-O-tetradecanoylphorbol 13-acetate (TPA) study was performed on mice genetically depleted of Dlx3 in skin epithelium (Dlx3cKO). Dlx3cKO mice developed significantly more tumors, with more rapid tumorigenesis compared to control mice. In Dlx3cKO mice treated only with DMBA, tumors developed after ~16 weeks suggesting that loss of Dlx3 has a tumor promoting effect. Whole transcriptome analysis of tumor and skin tissue from our mouse model revealed spontaneous activation of the EGFR-ERBB2 pathway in the absence of Dlx3. Together, our findings from human and mouse model system support a tumor suppressive function for DLX3 in skin and underscore the efficacy of therapeutic approaches that target EGFR-ERBB2 pathway.
- Published
- 2021
- Full Text
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