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sec id="st1"titleOBJECTIVES/titleTo examine: 1) whether exposure to secondhand smoke (SHS) at home is associated with symptoms of self-reported illness among mother-child pairs (MCPs); and 2) the relationship between low socio-economic status and SHS exposure and the role these play as obstacles to the reduction of risk of illness in MCPs./secsec id="st2"titleMETHOD/titleA cross-sectional study was conducted in Rajshahi District, Bangladesh, from May to July 2017. A total of 541 MCPs were interviewed./secsec id="st3"titleRESULTS/titleThe prevalence of SHS exposure at home in our sample data was 49.0%. SHS exposure was found to be associated with a higher likelihood of any self-reported rhinitis, any respiratory symptoms and any reproductive health problems among mothers. SHS exposure in children was found to be associated with a higher likelihood of any self-reported rhinitis and food sensitisation, any respiratory symptoms and otitis media. Our findings also suggested that although SHS had an independently adverse effect on MCPs, wealth moderated the likelihood of illness./secsec id="st4"titleCONCLUSIONS/titleMCPs who were both poor and exposed to SHS were uniquely disadvantaged in terms of their poor health conditions than MCPs who were wealthier and exposed to SHS./sec.

Objective\ud This study assessed the relationship between urbanization and the double burden of malnutrition (DBM) in Peru.\ud \ud Methods\ud A cross-sectional analysis of the Demographic and Health Survey (2009 to 2016) was conducted. A DBM “case” comprised a child with undernutrition and a mother with overweight/obesity. For urbanization, three indicators were used: an eight-category variable based on district-level population density (inhabitants/km2), a dichotomous urban/rural variable, and place of residence (countryside, towns, small cities, or capital/large cities).\ud \ud Results\ud The prevalence of DBM was lower in urban than in rural areas (prevalence ratio [PR] 0.70; 95% CI: 0.65-0.75), and compared with the countryside, DBM was less prevalent in towns (PR 0.75; 95% CI: 0.69-0.82), small cities (PR 0.73; 95% CI: 0.67-0.79), and capital/large cities (PR 0.53; 95% CI: 0.46-0.61). Using population density, the adjusted prevalence of DBM was 9.7% (95% CI: 9.4%-10.1%) in low-density settings (1 to 500 inhabitants/km2), 5.9% (95% CI: 4.9%-6.8%) in mid-urbanized settings (1,001 to 2,500 inhabitants/km2), 5.8% (95% CI: 4.5%-7.1%) in more densely populated settings (7,501 to 10,000 inhabitants/km2), and 5.5% (95% CI: 4.1%-7.0%) in high-density settings (>15,000 inhabitants/km2).\ud \ud Conclusions\ud The prevalence of DBM is higher in the least-urbanized settings such as rural and peri-urban areas, particularly those under 2,500 inhabitants/km2.

Polychlorinated biphenyls (PCBs) have been prohibited for two decades in Japan and Taiwan. The aim of this study was to compare the PCB congeners in maternal and cord serum between two countries. Our study subjects were 248 and 100 mother-child pairs in Japan and Taiwan. The measured levels of 23 serum PCB congeners between two countries were analyzed using gas chromatography–electron capture negative ionization quadrupole mass spectrometry (GC-NICI-qMS). The statistical comparisons were conducted by Student's t-test and principal component analysis with further stratification by maternal age and parity. The maternal total PCBs levels in Japan (426 ± 244 pg/g wet wt) were significantly higher than those in Taiwan (254 ± 155 pg/g wet wt), and the similar results were found in cord total PCBs levels (97 ± 76 and 58 ± 87 pg/g wet wt). It showed different distributions of PCB congeners between two countries. Whether in maternal or cord serum, the CB138, CB153 and CB180 were the highest detectable congeners whether in Japan or Taiwan. And, the CB66, CB99, CB206 and CB209 were only detected in maternal serum of Taiwan. The women of advanced maternal age had higher levels of PCB congeners, especially in Taiwan, and the primiparous women had higher levels of PCB congeners in two countries. In summary, the PCB congeners in Japan's mother-child pairs were with higher levels and different distributions when compared to those in Taiwan, and the maternal age and parity were important factors associated with the PCB levels.

Background: Malnutrition is a public health problem, with 45% of child deaths attributed to undernutrition. Aim: The aim of this study was to investigate the presence of the double burden of malnutrition in children-mother pairs in Benin. Methods: An analytical cross-sectional approach was used to collect data from 408 randomly selected children aged 0–59 months and their mothers. A pretested questionnaire was used to collect information on demographic and socioeconomic status, and anthropometric measurements were used to estimate the nutritional status of children and body mass indexes of the mothers. The World Health Organization Anthro software was used to estimate Z-scores whereas SPSS version 25 was used for statistical analysis. Descriptive and regression analysis were carried out on the data. Results: The mean age of children was 24.45 ± 14.9 months and 53.2% were girls. The mean age of mothers was 27.99 ± 6.99, 93.1% were married and approximately 91.7% of households had a monthly income of US$60. Prevalence of wasting in children was 9.8% and stunting was 29.7%. The mean waist circumference of mothers was 79.95 ± 10.35 cm and 19.3% were classified as high metabolic risk. Body mass index measurements showed that 16.9% of mothers were overweight and 7.4% obese. Conclusions: Stunting and wasting were highly prevalent. There is a coexistence of underweight and overweight in Grand-Popo, Benin. The results drew attention to the need for coordination of preventive interventions to focus on the household food distribution.

BACKGROUND: Humans are ubiquitously exposed to flame retardants, including organophosphate esters (OPEs), through direct contact with consumer products or exposure through household dust. Children are at increased risk because of their proximity to dust, hand-to-mouth activity, and the importance of childhood as a critical period in neurodevelopment. OBJECTIVES: To quantify differences in exposure levels between mothers and children (three to six years of age), we analyzed urinary metabolites of OPEs. We additionally assessed the ability of silicone wristbands (measuring ambient exposure) to predict urinary metabolite concentrations. METHODS: We selected 32 mother and child dyads from an existing cohort. Participants provided baseline urine samples and wore wristbands for one week. After the first week, they returned their wristbands and provided a second urine sample. During the second week, participants wore a second wristband that they returned at the end of week two with a third and final urine sample. RESULTS: We found significantly higher levels of bis(1,3-dichloro-2-propyl) phosphate (BDCIPP) (p < 0.001) and lower levels of bis(1-chloro-2-isopropyl) I-hydroxy-2-propyl phosphate (BCIPHIPP) (p < 0.001) in children’s urine samples compared to mothers’ samples at baseline. We found that triphenylphosphate (TPHP), tris(1,3-dichloroisopropyl) phosphate (TDCIPP), and tris(1-chloro-2-propyl) phosphate (TCIPP) measured in wristbands predicted their respective metabolite levels in urine. CONCLUSION: Children had higher levels than mothers for two of six flame retardant metabolites measured in urine. Generally, wristband measurements positively predicted internal dose. As little is known about the health effects of OPEs on child development, future research is needed to determine the impact of differential exposure.

OBJECTIVES: Adult studies have indicated that the gut microbiome plays a role in blood pressure regulation, however, there have been no studies on this association in children or mother-child pairs. This study's aim is to determine whether gut microbiome composition is associated with systolic blood pressure (SBP) in children and their mothers 5 years after birth. METHODS: We obtained stool samples from Canadian mother-child pairs (n = 164 children, 168 mothers) on average 5 years after birth in the prospective Gen3G pre-birth cohort. We characterized the microbiome using 16S rRNA gene sequencing of the V4 region. We categorized SBP (measured automatically, in duplicate) into tertiles separately for mothers and children and used the lowest tertile as the reference group. We fitted beta-binomial regression models to identify differentially abundant (FDR P

Little is known about household exposures to respiratory pathogens in HIV-exposed uninfected children (HEU) in Indian families. This case series investigates the nasopharyngeal carriage of Streptococcus pneumoniae, Staphylococcus aureus, and respiratory viruses at multiple points in three mother child pairs: (1) an HIV-infected child and mother, (2) an HEU child and HIV-infected mother, and (3) an HIV-unexposed uninfected (HUU) child and mother. Nasopharyngeal carriage densities of Streptococcus pneumoniae and Staphylococcus aureus were higher in mothers and children living in HIV-affected households, regardless of the child's HIV status. Maternal HIV and ART status impact these household exposures.

Introduction: Micronutrient deficiencies and malnutrition during the first 1000 days of life can have lifelong effects on the physical and cognitive development of the child. Lebanon, host of the world's highest per capita number of refugees, is becoming increasingly vulnerable to micronutrient deficiencies and facing a protracted crisis. Up-to-date, there is a lack of studies assessing hidden hunger, such as anemia and its determinants, particularly in a fragile setting.Material and methods: A cross-sectional survey was applied to 539 mother-child pairs of Syrian refugees and Lebanese host communities attending primary health care centers in 6 vulnerable areas of Greater Beirut, Lebanon between July and September 2018. The interview was completed by 476 pairs consisting of women of reproductive age (15–49 years) and children (0–59 months). The questionnaire gathered data on socio-economic characteristics, infant and young child feeding practices, and nutritional status. Hemoglobin concentrations were measured using the HemoCue Hb301 + analyzer. Data analysis used descriptive statistics, t-test, and chi-square test.Results: Overall, 45.4% of the women were lactating (LW), 16.6% pregnant (PW), and 38.0% non-pregnant non-lactating (NP/NL). The vast majority of the infants were ever breastfed (96.6%) and received colostrum (90.5%); however, more than half of the infants were offered pre-lacteal feedings (55.0%) and only one third were breastfed within the first hour after birth (35.2%). The rate of exclusive breastfeeding under six months was 22.2%. About half of the children were predominantly breastfed under six months (50.4%). Long-term breastfeeding up to the age of 1 year (47.2%), or even 2 years (19.2%), was practiced in line with the WHO recommendations. Anemia rates among mothers were significantly higher among NP/NL compared to PW and LW (26.1%, 17.7%, 18.8%, respectively, p < 0.05). Whereas, anemia rates among children were significantly higher in LW compared to NP/NL and PW (43.0%, 30.0%, 21,5%, respectively, p < 0.01). Among breastfed children, maternal anemia was significantly higher among anemic children (25.8%) compared to non-anemic children (14.0%, p < 0.05). Mild anemia of children under six months was significantly higher among overweight and obese mothers compared to mothers with a healthy BMI (67.7% vs 32.3%, p < 0.05).Conclusions: Despite an almost universal initiation of breastfeeding, early onset and exclusive breastfeeding under six months were low. Higher anemia rates were found among breastfed children and this was significantly associated to the maternal nutritional- and anemia status. Further analysis is required to examine the determinants of anemia and breastfeeding in this setting.

Objective: Dental caries is both an infectious and transmissible disease. Maternal transfer of Mutans Streptococci occurs at an early age and is important in the initiation of dental caries in children. The aim of this study was to identify certain strains of Streptococcus mutans in mother-child pairs, of children with early childhood caries. Study design: Sixty mother-child pairs of healthy children aged 18–36 months were selected. Mothers with high levels of Streptococcus mutans in their saliva and only children with ECC were included. Dental plaque samples were collected from mother-child pairs. The plaque samples were stored, transferred to the laboratory and analyzed for Streptococcus mutans strains c, f, e and k, present in mother-child pairs using Real time Polymerase Chain Reaction (PCR) technique. Data obtained was subjected to statistical analysis for level of similarity in Streptococcus mutans strains present in mother-child pairs. Results: A similar distribution of Streptococcus mutans strains c, f and k was identified in 28 mother-child pairs. Streptococcus mutans strain e was seen in 18 pairs. Conclusion: Less than 50% of mother-child pairs showed similarity in distribution of Streptococcus mutans strains.

Prenatal maternal stress predicts subsequent elevations in youth depressive symptoms, but the neural processes associated with these links are unclear. This study evaluated whether prenatal maternal stress is associated with child brain development, and adolescent depressive symptoms using a prospective design with 74 mother child pairs (40 boys). Maternal stress was assessed during pregnancy, child cortical thickness at age 7, and depressive symptoms at age 12. Prenatal maternal stress was associated with less cortical thickness primarily in frontal and temporal regions and with elevated depressive symptoms; child cortical thickness additionally correlated with adolescent depressive symptoms. The observed associations are consistent with the possibility that cortical thickness in superior frontal regions links associations between prenatal maternal stress and adolescent depressive symptoms.

Sustainable Development goal 2 focusses on preventing all forms of malnutrition by 2030. Therefore, it is important to understand the double burden of malnutrition (DBM) in mother-child pairs in India. This study attempted to explore the coexistence of DBM among mother-child pairs in India. Also, this study tries to explore various factors associated with DBM in India. The study utilized data from national representative cross-sectional fourth round of National Family Health Survey (NFHS-4) conducted in 2015–16. The study used anthropometric indices such as height-for-age, weight-for-height, and weight-for-age to examine the nutritional status of children aged 0–59 months. Further, the study used body mass index (BMI) of women and classified according to WHO cut-off values (normal BMI: 18.5 to

We measured urinary phthalate metabolites, including di-n-butyl phthalate (DnBP), di-isobutyl phthalate, benzyl butyl phthalate (BBzP), and di(2-ethylhexyl) phthalate (DEHP), from 178 school-aged children and their 284 family members using gas chromatography-mass spectrometry, and we calculated daily phthalate intakes. The highest median levels of phthalate metabolites were for mono-isobutyl phthalate in all participants except schoolchildren, where the highest levels were for mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP). Comparing the schoolchildren with their parents, the schoolchildren had significantly higher urinary metabolites for MEOHP, mono-(2-ethyl-5-carboxypentyl) phthalate, and EDEHP. Regarding daily intakes, the schoolchildren had significantly higher daily intakes of DnBP, BBzP, and EDEHP. All phthalate metabolite and sums of metabolite levels in the schoolchildren were positively correlated with their mothers' levels, except for MEHP, whereas fathers were less correlated with their children. The DEHP intake in this study was higher than that of most other studies. Moreover, 10% of the children and 3% of the adults exceeded the Reference Dose (RfD) value (20 mu g/kg/day) of the U.S. Environmental Protection Agency, which indicates that it is important to focus on children's DEHP exposure because the children exceeded the RID more than adults among the same families who shared similar exposure sources. Our results will contribute to considerations of the regulations for some phthalates and the actual phthalate exposure levels in the Japanese population. (C) 2015 Elsevier GmbH. All rights reserved.

Objectives This study aimed to examine socio-economic disparities in mother-child pairs' self-reported and cotinine-measured secondhand smoke (SHS) exposure at home. Study design This is a cross-sectional study. Methods This study was conducted in the Rajshahi district of Bangladesh from May to July 2017. A total of 541 mother-child pairs were interviewed for self-reported measured SHS; cotinine-measured saliva test was performed on 263 mothers and 236 children. Mother-child pairs' SHS exposure at home was the outcome of interest. Results Overall self-reported prevalence of SHS exposure at home was 49.0%. Self-reported SHS exposure among mothers (50.2%) and children (51.3%) were lower than the prevalence rate of cotinine-measured exposure for mothers (60.5%) and their children (58.9%). Maternal rich bands of wealth were found to be associated with lower likelihood of self-reported (adjusted odds ratio [AOR] = 0.59, 95% confidence interval [CI] = 0.35–0.99) and cotinine-measured SHS exposure among mothers (AOR = 0.17, 95% CI = 0.08–0.37). Maternal rich bands of wealth were also found to be associated with lower likelihood of cotinine-measured SHS exposure among children (AOR = 0.11, 95% CI = 0.07–0.26). Conclusions Socio-economic inequalities exist in exposure to SHS at home. Interventional approaches aimed at reducing SHS at home are urgently needed at public health and healthcare service level, with special focus given to the socioeconomically disadvantaged groups.

The worldwide ban of several formulations of brominated flame retardants has caused an increase in the production of organophosphorus flame retardants (PFRs) to meet the existing fire regulations for a wide range of household products. This biomonitoring study surveys the occurrence of the metabolites from PFRs and related plasticizers (dialkyl and diaryl phosphates; DAPs) in urine from a Norwegian mother–child cohort (48 mothers and 54 children). Concentrations of DAPs were higher in the children than in their mothers (Wilcoxon signed-rank test p = 0.001). Median urinary concentrations of diphenyl phosphate (DPHP) were 1.1 and 0.51 ng/mL in children and mothers, respectively, followed by bis(1,3-dichloro-2-propyl) phosphate (BDCIPP) with medians of 0.23 and 0.12 ng/mL, respectively. Detection frequencies for bis(2-butoxyethyl) phosphate (BBOEP) in urine from children and mothers were 32 and 1%, respectively (median

The mercury content in hairs was determined in the framework of the European funded projects COPHES and DEMOCOPHES to test the feasibility of an EU-HBM (Human Biomonitoring) approach generating comparable data. The aim of the Slovak participation in DEMOCOPHES was to obtain and contribute the Slovak data to the harmonization of Human Biomonitoring. Pre-analytical and analytical phase for mercury in hair measurements and activities developed for the harmonization analysis within COPHES/ DEMOCOPHES projects were conducted under a strict quality assurance program (QA/QC). Total mercury in hair was determined by thermal decomposition-gold amalgamation atomic absorption spectroscopy (AMA- 254 Advanced Mercury Analyzer.) Two interlaboratory comparison investigations (ICIs) and two external quality assessment schemes (EQUAS) were conducted before the beginning of the COPHES/DEMOCOPHES projects. The laboratory successfully completed both ICIs and EQUAS schemes and was allowed to analyze all DEMOCOPHES samples of the Slovak Republic. In summary, 129 mother-child pairs were recruited to pilot study of DEMOCOPHES in Slovakia from two different locations representing urban and rural environment. The analyzed data from Slovakia showed relationship between frequency of fish meals consumption (especially sea fish and sea food products) and mercury concentrations in hair of mothers and children. The exposure levels for a sampled population in Slovakia (children 0.092 (0.080-0.106) [μg.g-1], mother 0.130 (0.113-0.148) [μg.g-1]) are below the EU level (children 0.145 (0.139-0.151) [μg.g-1], mother 0.225 (0.216-0.234) [μg.g-1]) up to an order of magnitude lower than the limit medically important (FAO/WHO health-based guidance value of 2.3 μg.g-1 JECFA - Joint FAO/WHO Expert Committee on Food Additives).

This study aimed to correlate the cariogenic responsiveness of biofilms generated from the saliva of mothers and children. The mother-child pairs were classified according to the children's caries levels: caries-free, early childhood caries (ECC) or severe ECC. Microcosm biofilms were grown on enamel discs for 10 days. Factors under evaluation were caries experience levels, inoculum source (mothers and children) and growth conditions including cariogenic challenge (growth medium provided with and without sucrose) and no cariogenic challenge (growth medium sucrose-free). Statistical analysis was performed with ANOVA and Tukey's test, and the Spearman correlation test. Regular sucrose exposure resulted in a higher surface hardness change (%SHC). The correlation between biofilms formed from saliva of mother-child pairs was significant regarding pH, total aciduric microorganisms and lactobacilli counts under cariogenic challenge. Biofilm growth originating from mother-child pairs under regular sucrose exposure promoted the same cariogenic response independently of caries experience and the microbiological profile of the donors.

Background: Disruption of thyroid homeostasis has been indicated in human studies targeting effects of persistent organic pollutants (POPs). Influence on the maternal thyroid system by POPs is of s...

BACKGROUND: There are limited data on the associations between maternal or newborn and child exposure to perfluoroalkyl acids (PFAAs), including perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS). This study provides an opportunity to assess the association between PFAA concentrations in mother-child pairs in a population exposed to PFOA via drinking water. \ud OBJECTIVES: We aimed to determine the relationship between mother-child PFAA serum concentrations and to examine how the child: mother ratio varies with child's age, child's sex, drinking-water PFOA concentration, reported bottled water use, and mother's breast-feeding intention. \ud METHODS: We studied 4,943 mother-child pairs (children, 1-19 years of age). The child: mother PFAA ratio was stratified by possible determinants. Results are summarized as geometric mean ratios and correlation coefficients between mother-child pairs, overall and within strata. \ud RESULTS: Child and mother PFOA and PFOS concentrations were correlated (r = 0.82 and 0.26, respectively). Up to about 12 years of age, children had higher serum PFOA concentrations than did their mothers. The highest child: mother PFOA ratio was found among children 5 years of age had significantly higher PFOA and PFOS child: mother ratios than did girls. \ud CONCLUSION: Concentrations of both PFOA and PFOS tended to be higher in children than in their mothers. This difference persisted until they were about 12 years of age for PFOA and at least 19 years of age for PFOS.

Bisphenol A (BPA) may alter endocrine functions, and human exposure is widespread. In the Duisburg birth cohort study the influence of several contaminants was examined on the neuropsychological and pubertal development of children. This study reports the biomonitoring results on BPA within the 6-yr follow-up study (children's age 6-8 yr). Total BPA and free (unconjugated) BPA concentrations in 208 urine samples of 104 mother-child pairs were measured. For quality control, total BPA was analyzed by two independent laboratories. BPA was detected in all urine samples, while free BPA was observed above the limit of quantification (LOQ) in only 33 samples. Total BPA concentrations were significantly associated between the two laboratories. BPA concentrations (median; range) tended to be higher in children than in mothers but the difference was not significant. Total BPA levels in children and mothers correlated at low levels but significantly to each other. Multivariate linear regression analysis revealed positive associations between creatinine and the BPA concentrations and a negative association with German nationality (mothers only). Evidence indicates that BPA exposure is omnipresent but levels in mother-child pairs are low. Only small amounts (less than 16%) were detectable as free (unconjugated) BPA. Analytical reliability is high even at such low levels, provided that external contamination is minimized.

Dietary habits established in early childhood contribute to lifelong dietary pattern and the development of early risk factors for disease in adulthood. Although a large body of epidemiologic data from Western countries show that the dietary pattern of children is influenced by maternal socio-economic and lifestyle characteristics, information on this topic in non-Western countries is absolutely lacking. The present study identified dietary patterns among infants aged 16-24 months, and then examined the influence of maternal socio-economic and lifestyle characteristics on identified dietary patterns. Subjects were 758 Japanese mother-child pairs. Dietary data of infants were collected from the mothers using a questionnaire. Dietary patterns were extracted from the consumption of 15 foods (times week(-1)) by cluster analysis. The following two dietary patterns were identified: 'fruits, vegetables and high-protein foods' (n = 483) and 'confectionaries and sweetened beverages' (n = 275) patterns. After adjustment for all other predictors, maternal educational level, number of infants' siblings and maternal dietary patterns were independently associated with dietary patterns of infants. Infants whose mothers had a higher educational level and the 'rice, fish and vegetables' dietary pattern were less likely to belong to the 'confectionaries and sweetened beverages' pattern, whereas infants whose mothers had a higher number of children and the 'wheat product' dietary pattern were more likely to belong to the 'confectionaries and sweetened beverages' than the 'fruits, vegetables and high-protein foods' pattern. In conclusion, the mother's socio-economic position and dietary patterns were associated with the dietary patterns of infants in the Japanese pairs as observed in the Western populations.

Familiare Belastungen sowie mangelnde Bewaltigungsressourcen von Muttern werden in unterschiedlichen theoretischen Modellen als Risikofaktoren fur die kindliche Entwicklung diskutiert. In vorliegender Arbeit wurden Selbstangaben der Mutter zu Belastungen sowie Bewaltigungsressourcen und Selbstangaben ihrer Kinder zur gesundheitsbezogenen Lebensqualitat (N=564 Mutter-Kind-Paare) zu 2 Messzeitpunkten (Beginn und Ende einer 3-wochigen Mutter-Kind-Masnahme) untersucht. Die Ergebnisse eines Strukturgleichungsmodells zeigen, dass zum einen familiare Belastungen direkt die kindliche gesundheitsbezogene Lebensqualitat beeinflussen und zum anderen ihr Einfluss uber das elterliche Kompetenzgefuhl beziehungsweise das psychische Befinden der Mutter mediiert wird. Auch Veranderungen im psychischen Befinden und im elterlichen Kompetenzgefuhl der Mutter hangen in Abhangigkeit vom Geschlecht des Kindes geringfugig, aber signifikant mit der Veranderung der kindlichen gesundheitsbezogenen Lebensqualitat zusammen. Die Analyse unabhangiger Daten zeigt somit, dass die Forderung spezifischer personeller Ressourcen von Muttern mit einer geringen aber signifikanten Veranderung der kindlichen gesundheitsbezogenen Lebensqualitat einhergeht.

Early childhood caries results in a considerable burden of pain and suffering as well as poorer general health. Streptococcus mutans (serotypes c , e and f ) and Streptococcus sobrinus (serotype d and g ) are the species closely associated with dental caries. The exact age at which their colonization occurs in children is controversial. The objective of this study was to detect S. mutans and S. sobrinus in plaque samples of Mongolian mother-child pairs by PCR and to compare their presence with the caries status. Dental examination and caries risk assessment using the Cariostat ® carried out on 320 children aged 6–30 months and their mothers. The presence of S. mutans and S. sobrinus was checked by PCR. The caries prevalence and DEFT scores of mothers enrolled in the present study were 98% and 11.5±0.7, respectively. In children, the prevalence and deft scores of the 6–18-month-olds were 29% and 1.3±0.2 while those of the 19–30-month-olds were 59% and 3.4±0.4 correspondingly. Twenty nine percent of the 6–18-months old children of high-risk mothers and 53.1% of the 19–30-months old children of high-risk mothers had high caries risk ( P P S. mutans and S. sobrinus was 79% and 33%, respectively; 54% harbored S. mutans alone, 8% harbored S. sobrinus alone, 25% harbored both strains. In children, 45% were positive for S. mutans alone, 9% were positive for S. sobrinus alone, 18% were positive for both strains. Either or both strains were detected in 67.3% of 6–18-months old children and 76.5% of 19–30-months old children. In conclusion, our results showed that S. mutans and/or S. sobrinus first colonized infants' teeth from 6–18 months, and the colonization increased with increasing age, so that by 30 months of age, 76.5% of children harbored the bacteria.

A sample of 95 mother-child pairs provided evidence that plasma haptoglobin (Hp) types are inherited in an unusual manner by children with Down's syndrome. Homozygous mothers gave birth to more homozygotes and fewer heterozygotes than expected. Among offspring of heterozygous mothers, the frequencies were distributed essentially as expected. No abnormality was found in a normal control sample of 151 mother-child pairs. Using this material it was demonstrated that the anomalous Hp inheritance in Down's syndrome was not due simply to an increase in maternal age when the children were born.

The aim of this study was to determine the presence of Streptococcus mutans or Streptococcus sobrinus in Cariostat-inoculated plaque samples obtained from Japanese mother-child pairs through a conventional PCR technique and to establish the presence of these bacteria and caries risk. Oral examination and caries risk assessment using the Cariostat ® were carried out on 168 children, aged 6–31 months, and their mothers. The presence of S. mutans and S. sobrinus in Cariostat-inoculated plaque samples was checked through PCR and tested for relevance with caries risk. A significant correlation ( P S. mutans or S. sobrinus in plaque samples from their children in the 19–31-month-old age range. However, no significant relationship found between the presence of either strain in the plaque of younger children (6–18 months) and caries risk of mothers. Likewise, high caries risk was seen in 49.1% of the 19–31-month-old children of highrisk mothers ( P P S. mutans and S. sobrinus in plaque samples obtained from mothers and their children through conventional PCR techniques.

The major route of early acquisition of mutans streptococci in humans is a vertical transmission from mother to child. The purpose of this longitudinal study was to examine the acquisition, distribution and persistence of Streptococcus mutans and Streptococcus sobrinus in children whose mothers harbored both species and to study the caries incidence in relation to colonization of these bacteria. Fifteen mother-child pairs were followed during the child’s first 7 years. Stimulated salivary samples were taken from the mothers and the children. Plaque samples were also collected from the teeth and the tongue of the children. The samples were analyzed by cultivating techniques together with genomic fingerprinting and hybridizing. The caries experience was evaluated on the sampling occasions and retrospectively using the records of caries registrations from the community clinics. During the 7-year period 10 of the 15 children acquired mutans streptococci. Only 4 of them were colonized by both S. mutans and S. sobrinus despite the fact that their mothers harbored both species. In 2 of the children S. sobrinus was found later than S. mutans. A total of 26 genotypes were found in the children and 9 of them were identical to their mothers. New genotypes and a gain-loss pattern were noted especially in the children but also in their mothers. The groups of teeth first positive for the two species were the deciduous molars. The caries experience was low during the study period with 8 children showing no caries.

Low-level exposure to polychlorinated biphenyl-153 (PCB-153) and dichlorodiphenyldichloroethylene (p-p′-DDE) can impair fetal growth; however, the exposure–response relationship and effect modifiers of such association are not well established. This study is an extension of an earlier European meta-analysis. Our aim was to explore exposure–response relationship between PCB-153 and p-p′-DDE and birth outcomes; to evaluate whether any no exposure–effect level and susceptible subgroups exist; and to assess the role of maternal gestational weight gain (GWG). We used a pooled dataset of 9377 mother–child pairs enrolled in 14 study populations from 11 European birth cohorts. General additive models were used to evaluate the shape of the relationships between organochlorine compounds and birth outcomes. We observed an inverse linear exposure–response relationship between prenatal exposure to PCB-153 and birth weight [decline of 194 g (95% CI −314, −74) per 1 μg/L increase in PCB-153]. We showed effects on birth weight over the entire exposure range, including at low levels. This reduction seems to be stronger among children of mothers who were non-Caucasian or had smoked during pregnancy. The most susceptible subgroup was girls whose mothers smoked during pregnancy. After adjusting for absolute GWG or estimated fat mass, a reduction in birth weight was still observed. This study suggests that the association between low-level exposure to PCB-153 and birth weight exists and follows an inverse linear exposure–response relationship with effects even at low levels, and that maternal smoking and ethnicity modify this association. Keywords: Biomarkers of exposure, Persistent organic pollutants, Polychlorinated biphenyls (PCBs), Dichlorodiphenyldichloroethylene (DDE), Birth weight

Aim: Children's patterns of sugar consumption and dental health status have been associated to their mothers' patterns. As a liking for sweetness has been related to caries levels, this study was carried out to analyse the relationship between sweetness preference and caries experience in mother/child pairs. Participants and methods: A cross-sectional survey was conducted on a sample of low socio-economic mothers and their 4–5-year-olds from Maringa-Pr, Brazil. Mothers' and children's preference for sugar was measured using the Sweet Preference Inventory. Their caries experience was assessed according to World Health Organization (WHO) guidelines. Results: High levels of sweetness preference and caries experience were found in the sample studied. Although not strong, a significant correlation in patterns of sweetness preference (r=0.12, P < 0.05) and caries experience (r = 0.14, P < 0.05) between mother/child pairs was identified. Among the social-demographic variables, only family income was statistically associated with the children's preference for sweetness (P < 0.05). No significant association between sweetness preference and caries experience could be established. Conclusion: The findings of this study suggest that mothers may play an important role on the establishment of their children dietary preferences and patterns of dental health. Also, that the socio-economic status may influence children's preference for sugar.

Egypt has seen an increase in overweight and obesity over the last two decades. This study aims to identify current prevalence and associated risk factors for an intra-household double burden of ov...

Proxemic communication (behaviours and relations of human interaction) offered by the mother in an HIV-infected mother-child pair during infancy was analysed to determine which proxemic factors promote the exchange of affection. This study, conducted in an experimental laboratory in 2007, included mother-child pairs in which the mother was HIV-positive and the children, under 6 months of age, were born exposed to the virus. Video recordings of the mother-child interaction were analysed according to proxemic factors. Of the 364 interactions recorded for analysis of proxemic communication between the mother-child pair, the most significant proxemic factors were axis, contact behavior, visual code, and tone of voice. The mothers developed communicative strategies such as closer proximity, touch, vocalisation, and smiling to promote the exchange of affection when engaging in maternal care.

Background Mother-to-child transmission is the predominant route of HIV-1 infection in children. Prevention strategies are available in industrialized countries, but are limited in developing countries. Defining pertinent characteristics of transmitted HIV-1 from mother-child pairs, where the actually transmitted virus has been traced, is important in creating better preventive measures against transmission of HIV-1.

Recent researches have focused on xylitol as convenient and effective method to inhibit cariogenic bacteria. The purpose of the present study is to assess the effect of xylitol on plaque accumulation, caries activity and salivary Streptococcus Mutans in a group of Saudi mother-child pairs. Materials and Methods: The study sample included 60 mother and child pairs selected on the basis of having high salivary streptococcus mutans levels. The study sample was randomly divided into experimental group (30 pairs) and control group(30 pairs). The experimental group was given xylitol treatment and the controls received fluoride varnish. Both groups were examined to assess caries, plaque and salivary streptococcus mutans levels. Xylitol treatment in the form of chewing gum for mothers and tablets for children was consumed three times/day for three months. All subjects received oral hygiene instructions, dietary counseling and restorative treatment. The results showed that the number of mothers and children with high streptococcus levels in the experimental group decreased to a statistically significant level at the end of the three month period, similarly, the control mothers showed the same trend. A statistically significant decrease in plaque scores was evident only among the children's experimental group. The caries level of children and mothers showed no statistically significant differences between the experimental and control groups. The factors which significantly affected the streptococcus mutans count in children after three months were the child's dmft at baseline the preventive method used and the mother' salivary streptococcus mutans level.

This article reports a longitudinal study of developing communication in two profoundly deaf preschool boys growing up in oral deaf families who use oral English as their primary language. The children were videotaped in play interactions with their profoundly deaf mothers. The nature of the gestural communication used by the dyads is the focus of interest in this article. In contrast to hearing mothers of deaf children, the two mothers used extensive gestures to accompany their speech, including rich and varied gesture sequences. The children also developed a repertoire of gestures that, in most respects, mirrored the properties of the input gesture they received. The results provide a window on the natural ontogenesis of a compensatory gestural system.

The relationship between dental caries and mutans streptococci (ms) in children and their mothers is a key factor in the prevention of early childhood caries. Changes in early childhood caries over time in Mexican children have not been well documented. Objective: to compare the dental caries indices and presence of ms in 3- to 5-year-old children and their mothers in two surveys, the first one carried out in 1996 and the second one in 2007. Study design: cross-sectional study. Results: The first survey included 102 mother-child dyads and the second 103. Dental caries index dmfs was 7.17 (± 10.9) and 4.58 (± 7.1) in the first and second survey, respectively (p0.05). Children with SECC showed higher counts of ms than children without this condition. A high caries index in mothers was associated with higher caries experience in their children. Conclusion: the compassions of the surveys indicated a decline in dental caries indices over an 11-year period. High caries experience in the mother had a negative impact on the oral condition of the child.

Previous studies have suggested that mother-to-childtransmission of HIV-1 is often characterized by acquisi-tion of a homogeneous viral quasispecies in the infant [1-3], suggestive of a genetic bottleneck. In this study, wehave analyzed the molecular characteristics of transmittedHIV-1 viruses in a homogeneous population infected byCRF01_AE variants in Thailand.

BACKGROUND: Suspected incestuous paternity is encountered infrequently and investigation may be complicated by absence of the suspected father. Incest indices (IIs) can be calculated from microsatellite (STR) types of only a mother and child, but could be misleading. Therefore, the method was evaluated. STUDY DESIGN AND METHODS: Combined incest indices (CIIs) of 50 randomly mated (RM) mothers and their children were compared with those of 50 simulated incestuous (SI) mothers and their children. Each CII was calculated from 18 individual locus IIs. Combined indices were categorized as “diagnostic” ( 100 for RM and SI cases, respectively), “indicative” (CII was directionally correct but not erroneously diagnostic), and “misleading” (>1.0 in RM and

Background: Early childhood influences are important for the development of the allergic phenotype. In East Germany, tremendous lifestyle changes took place after 1990 and it can be hypothesized that the allergic phenotypes in mothers and their children are less similar than in West Germany. This was investigated in our study done in mothers and their 6-year-old children from East and West Germany in the year 2000. Methods: 1,393 mother-child pairs participated. A subgroup of 774 pairs gave blood for the determination of specific IgE. Regional differences in mother-child correlations and in prevalence of mother-child combinations with respect to allergic sensitization and disease were examined by logistic regression analysis. Results: The adjusted association in positive allergic sensitization between mothers and their children was not significant in East Germany (OR 1.23, 95% CI: 0.68–2.24) but highly significant in West Germany (OR 2.89, 95% CI: 1.73–4.80). The probability for the combination of ‘negative’ mother and ‘positive’ child was significantly higher in East than in West Germany. Conclusions: Mother-child transmission of atopy predisposition can even be cancelled by environmental changes.

Actinomyces naeslundii genospecies 2 (gsp-2) are members of the autochthonous oral flora. Chromosomal DNA fingerprinting (CDF) with SmaI revealed extensive genetic diversity among A. naeslundii gsp-2 strains within individual mothers and children. There was a low prevalence of genotype match among A. naeslundii gsp-2 strains between all mother and child pairs.

Blood and environmental samples, including a quarterly 6-day duplicate diet, for nine mother/child pairs from Eastern Europe have been monitored for 12 to >24 months with high precision stable lead isotope analysis to evaluate the changes that occur when the subjects moved from one environment (Eastern Europe) to another with different stable lead isotopes (Australia). The children were between 6 and 11 years of age and the mothers were between 29 and 37 years of age. These data were compared with an Australian control mother/child pair, aged 31 and 6 years, respectively. A rationale for undertaking this study of mother/child pairs was to evaluate if there were differences in the patterns and clearance rates of lead from blood in children compared with their mothers. Blood lead concentrations ranged from 2.1 to 3.9 microg/dl in the children and between 1.8 and 4.5 microg/dl in the mothers, but the mean of differences between each mother and her child did not differ significantly from zero. Duplicate diets contained from 2.4 to 31.8 microg Pb/kg diet; the mean+/- standard deviation was 5.5 +/- 2.1 microg Pb/kg and total daily dietary intakes ranged from 1.6 to 21.3 microg/day. Mean daily dietary intakes relative to body weight showed that the intake for children was approximately double that for the mothers (0.218 vs. 0. 113 microg Pb/kg body weight/day). The correlations between blood lead concentration and mean daily dietary intake either relative to body weight or total dietary intake did not reach statistical significance (p>0.05). Estimation of the lead coming from skeletal (endogenous) sources relative to the contribution from environmental (exogenous) sources ranges from 8 to 70% for the mothers and 12 to 66% for the children. The difference between mothers and children is not statistically significant (p = 0.28). The children do not appear to achieve the Australian lead isotopic profile at a faster rate than their mothers. These data provide evidence that the absorption or uptake of lead from dietary sources is similar in adult females and children of the age in this study. In spite of lower bone lead and faster bone remodeling and recycling in children compared with adult females, we see no differences between the mothers and their children in overall contribution of tissue lead to blood lead. Results from this study suggest that fractional absorption of ingested lead by children 6-11 years of age is comparable with absorption patterns observed among adult females in the 29-37-year-old age range. Because pharmacokinetic models apply a 40-50% absorption even for 7-year-old children, further investigations on fractional absorption of ingested lead by young children are warranted.Further investigations are especially needed in younger children than those who were subjects in the current study, particularly children in the 1-3-year-old age range. In addition, the effect of nutritional status and patterns of food intake on children's lead absorption require investigation, particularly given the increased prevalence of marginal nutritional status among low-income populations that are at increased risk of elevated blood lead levels. Images Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. Figure 6. Figure 7. Figure 8. Figure 9. Figure 10. Figure 11.

Address: 1Paediatrics and child Health, HIV Pathogenesis Programme, Doris Duke Medical Research Institute, UKZN, Durban, South Africa, 2HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Durban, South Africa, 3Institute of Massachusetts General Hospital, Massachusetts, Massachusetts, MA USA and 4Department of Pediatrics, University of Oxford, England, Oxford, UK * Corresponding author