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13. Cellular Antioxidant and Peroxidase Inhibition Activities of Decoctions from Different Parts of Triclisia gilletii (De Wild) Staner (Menispermaceae)

Author

Kapepula, Paulin Mutwale, primary, Trésor, Ondze Jacques, additional, Ranarivelo, Njakarinala, additional, Mouithys-Mickalad, Ange, additional, Franck, Thierry, additional, Kawayidiko, Michel Kasongo, additional, Mpanzu, Arthur Duki, additional, Frédérich, Michel, additional, Kabamba, Nadège Ngombe, additional, and Tsobo, Christophe Masiala, additional

Published
2022
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15. Muscle Derived Mesenchymal Stem Cells Inhibit the Activity of the Free and the Neutrophil Extracellular Trap (NET)-Bond Myeloperoxidase

Author

Franck, Thierry, Ceusters, Justine, Graide, Hélène, Mouithys-Mickalad, Ange, and Serteyn, Didier

Subjects
muscle derived mesenchymal stem cells, QH301-705.5, Muscles, animal diseases, Mesenchymal Stem Cells, ROS, Extracellular Traps, Article, Cell Degranulation, NET, neutrophils, Animals, myeloperoxidase activity, Horses, Biology (General), Reactive Oxygen Species, Peroxidase, Protein Binding
Abstract

Mesenchymal stem cells (MSCs) are known to migrate to tissue injury sites to participate in immune modulation, tissue remodelling and wound healing, reducing tissue damage. Upon neutrophil activation, there is a release of myeloperoxidase (MPO), an oxidant enzyme. But little is known about the direct role of MSCs on MPO activity. The aim of this study was to investigate the effect of equine mesenchymal stem cells derived from muscle microinvasive biopsy (mdMSC) on the oxidant response of neutrophils and particularly on the activity of the myeloperoxidase released by stimulated equine neutrophils. After specific treatment (trypsin and washings in phosphate buffer saline), the mdMSCs were exposed to isolated neutrophils. The effect of the suspended mdMSCs was studied on the ROS production and the release of total and active MPO by stimulated neutrophils and specifically on the activity of MPO in a neutrophil-free model. Additionally, we developed a model combining adherent mdMSCs with neutrophils to study total and active MPO from the neutrophil extracellular trap (NET). Our results show that mdMSCs inhibited the ROS production, the activity of MPO released by stimulated neutrophils and the activity of MPO bound to the NET. Moreover, the co-incubation of mdMSCs directly with MPO results in a strong inhibition of the peroxidase activity of MPO, probably by affecting the active site of the enzyme. We confirm the strong potential of mdMSCs to lower the oxidant response of neutrophils. The novelty of our study is an evident inhibition of the activity of MPO by MSCs. The results indicated a new potential therapeutic approach of mdMSCs in the inhibition of MPO, which is considered as a pro-oxidant actor in numerous chronic and acute inflammatory pathologies.

Published
2021

18. Effects of Juglone on Neutrophil Degranulation and Myeloperoxidase Activity Related to Equine Laminitis

Author

Mouithys-Mickalad, Ange, Storms, Nazaré, Franck, Thierry, Ceusters, Justine, de la Rebière de Pouyade, Geoffroy, Deby-Dupont, Ginette, and Serteyn, Didier

Subjects
reactive oxygen species, myeloperoxidase, General Veterinary, juglone, electron paramagnetic resonance spectroscopy, Veterinary Science, neutrophil degranulation, Original Research
Abstract

Experimental laminitis, characterized by a failure of the dermal–epidermal interface of the foot, can be induced in horses by the oral administration of a black walnut extract (BWE). In the early phase of this severe and painful disease, an activation of neutrophil occurs, with the release of myeloperoxidase (MPO), a pro-oxidant enzyme of neutrophils, in plasma, skin, and laminar tissue. Juglone, a naphthoquinone derivative endowed with redox properties, is found in walnuts and has been incriminated in this neutrophil activation. We report for the first time the inhibitory activity of juglone on the degranulation of neutrophils induced by cytochalasin B and formyl-methionyl-leucyl-phenylalanine as monitored by the MPO release (>90% inhibition for 25 and 50 μM). Moreover, it also acts on the peroxidase activity of MPO by interacting with the intermediate “π cation radical,” as evidenced by the classical and specific immunological extraction followed by enzymatic detection (SIEFED) assays. These results are confirmed by a docking study showing the perfect positioning of juglone in the MPO enzyme active site and its interaction with one of the amino acids (Arg-239) of MPO apoprotein. By chemiluminescence and electron paramagnetic resonance techniques, we demonstrated that juglone inhibited reactive oxygen species (ROS) and superoxide anion free radical produced from phorbol myristate acetate (PMA)-activated polymorphonuclear neutrophils (PMNs). These results indicate that juglone is not the trigger for equine laminitis, at least if we focus on the modulation of neutrophil activation.

Published
2021

19. In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy

Author

Kruse, Caroline-J., Stern, David, Mouithys-Mickalad, Ange, Niesten, Ariane, Art, Tatiana, Lemieux, Hélène, and Votion, Dominique-M.

Subjects
atypical myopathy, cell culture, Science, high-resolution respirometry, equine primary myoblasts, toxicity assays, Article
Abstract

Equine atypical myopathy is a seasonal intoxication of grazing equids. In Europe, this poisoning is associated with the ingestion of toxins contained in the seeds and seedlings of the sycamore maple (Acer pseudoplatanus). The toxins involved in atypical myopathy are known to inhibit ß-oxidation of fatty acids and induce a general decrease in mitochondrial respiration, as determined by high-resolution respirometry applied to muscle samples taken from cases of atypical myopathy. The severe impairment of mitochondrial bioenergetics induced by the toxins may explain the high rate of mortality observed: about 74% of horses with atypical myopathy die, most within the first two days of signs of poisoning. The mechanism of toxicity is not completely elucidated yet. To improve our understanding of the pathological process and to assess therapeutic candidates, we designed in vitro assays using equine skeletal myoblasts cultured from muscle biopsies and subjected to toxins involved in atypical myopathy. We established that equine primary myoblasts do respond to one of the toxins incriminated in the disease.

Published
2021

21. Cellular Antioxidant and Peroxidase Inhibition Activities of Decoctions from Different Parts of Triclisia gilletii (De Wild) Staner (Menispermaceae).

Author

Mutwale Kapepula, Paulin, Trésor, Ondze Jacques, Ranarivelo, Njakarinala, Mouithys-Mickalad, Ange, Franck, Thierry, Kawayidiko, Michel Kasongo, Mpanzu, Arthur Duki, Frédérich, Michel, Kabamba, Nadège Ngombe, and Tsobo, Christophe Masiala

Subjects
PEROXIDASE, ANTIOXIDANTS, TRACHEARY cells, ANTI-inflammatory agents, CHEMICAL fingerprinting, VEGETABLES
Abstract

Introduction: Nutrition is an important aspect of public health and the high intake of vegetables and fruits would greatly reduce the risk of diseases associated with oxidative damage. Triclisia gilletii, a traditional vegetable from Kongo Central area (DR. Congo), was studied for establishing the cellular antioxidant activity, the inhibition of a proinflammatory enzyme (MPO) activity of its different parts and their microscopic and Chromatographic features. Materials and Methods: The antioxidant activities and the inhibition effect on MPO activity of decoctions from leaves, root bark and stern bark of Triclisia gilletii were evaluated using in vitro cell-free, cell-based and enzymatic assays. In addition, to better characterize the plant, the microscopic features and chromatographic profiles of different parts of T. gilletii were determined. Results: Microscopically, leaf can be characterized by anomocytic stomata, spiral vessels, pluricellular non-glandular trichomes, root bark by pitted vessels, abundant starch grains, isodiametric sclereids, cristalline fibers, and stem bark by tracheids with bordered pits, sclereids, ovoids starches grains and pitted vessels. Aqueous extracts of all parts showed the best cellular antioxidant activities on ROS-induced chemiluminescence using L012 on HL 60 monocytes at the concentrations range of 0.1-2 μg/mL with the root bark being the most active plant part. Extracts showed more efficient MPO inhibitory activity measured by the SIEFED technique, with the stem bark being the most active. Antioxidant and anti-inflammatory activities were significantly higher and positively correlated with phytochemicals. Conclusion: The obtained bioactivities may justify the use of this plant as a traditional vegetable, potent nutraceutical and medicine. [ABSTRACT FROM AUTHOR]

Published
2022
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22. Microscopic Characteristics, Chromatographic Profiles and Inhibition of Peroxidase Activity of the Leaves of <i>Manihot esculenta</i> and <i>Manihot glaziovii</i>, Consumed as Traditional Vegetables

Author

Kapepula, Paulin Mutwale, primary, Mungitshi, Patricia Mbombo, additional, Tshitenge, Dieudonné Tshitenge, additional, Franck, Thierry, additional, Ngoyi, Dieudonné Mumba, additional, Kalenda, Pascal Dibungi T., additional, Tits, Monique, additional, Frédérich, Michel, additional, Ngombe, Nadege Kabamba, additional, and Mouithys-Mickalad, Ange, additional

Published
2021
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23. Prunella vulgaris L. seeds: a promising source of lipids, proteins, and original phenolic compounds presenting high antioxidant and anti-inflammatory activity

Author

Danthine, Sabine, primary, Paul, Aman, primary, Jansen, Olivia, primary, Ducrey, Aurélien, primary, Richel, Aurore, primary, Lognay, Georges, primary, Maesen, Philippe, primary, Kapepula, Paulin Mutwale, primary, Mouithys-Mickalad, Ange, primary, Franck, Thierry, primary, and Frédérich, Michel, primary

Published
2021
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30. Prunella vulgaris L. seeds: a promising source of lipids, proteins, and original phenolic compounds presenting high antioxidant and anti-inflammatory activity.

Author

Danthine, Sabine, Paul, Aman, Jansen, Olivia, Ducrey, Aurélien, Richel, Aurore, Lognay, Georges, Maesen, Philippe, Kapepula, Paulin Mutwale, Mouithys-Mickalad, Ange, Franck, Thierry, and Frédérich, Michel

Subjects
PHENOLS, ANTI-inflammatory agents, COMPOSITION of seeds, PLANT phenols, SEEDS, PROTEINS, PLANT polyphenols
Abstract

Copyright of Biotechnologie, Agronomie, Societe et Environnement is the property of Les Presses Agronomiques de Gembloux and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2022

36. The soluble curcumin derivative NDS27 inhibits superoxide anion production by neutrophils and acts as substrate and reversible inhibitor of myeloperoxidase

Author

Franck, Thierry, primary, Aldib, Iyas, additional, Zouaoui Boudjeltia, Karim, additional, Furtmüller, Paul G., additional, Obinger, Christian, additional, Neven, Philippe, additional, Prévost, Martine, additional, Soubhye, Jalal, additional, Van Antwerpen, Pierre, additional, Mouithys-Mickalad, Ange, additional, and Serteyn, Didier, additional

Published
2019
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37. Capillary electrophoresis, high-performance liquid chromatography, and thin-layer chromatography analyses of phenolic compounds from rapeseed plants and evaluation of their antioxidant activity

Author

Huang, Yang, primary, Jansen, Olivia, additional, Frédérich, Michel, additional, Mouithys-Mickalad, Ange, additional, Nys, Gwenaël, additional, Servais, Anne-Catherine, additional, Crommen, Jacques, additional, Jiang, Zhengjin, additional, and Fillet, Marianne, additional

Published
2018
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38. Flavonoid composition, cellular antioxidant activity and (myelo)peroxidase inhibition of a Bryonia alba L. (Cucurbitaceae) leaves extract

Author

Ielciu, Irina, primary, Mouithys-Mickalad, Ange, additional, Franck, Thierry, additional, Angenot, Luc, additional, Ledoux, Allison, additional, Păltinean, Ramona, additional, Cieckiewicz, Ewa, additional, Etienne, Delphine, additional, Tits, Monique, additional, Crişan, Gianina, additional, and Frédérich, Michel, additional

Published
2018
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39. In vitro Antileishmanial, Antitrypanosomal, and Anti-inflammatory-like Activity of Terminalia mollis Root Bark.

Author

Muganga, Raymond, Bero, Joanne, Quetin-Leclercq, Joëlle, Angenot, Luc, Tits, Monique, Mouithys-Mickalad, Ange, Franck, Thierry, and Frédérich, Michel

Subjects
ANTIPROTOZOAL agents, IN vitro studies, LEISHMANIASIS, HIGH performance liquid chromatography, AMPHOTERICIN B, ANTI-inflammatory agents, CHEMILUMINESCENCE assay, ANIMAL experimentation, HORSES, ANTIOXIDANTS, TANNINS, PLANT roots, BARK, PARASITIC diseases, TRYPANOSOMIASIS, PLANT extracts, REACTIVE oxygen species, PHARMACODYNAMICS
Abstract

This study aims at determining the in vitro antitrypanosomal, antileishmanial, antioxidant, and anti-inflammatory-like activities of Terminalia mollis root crude extracts. The antitrypanosomal and antileishmanial activities on Trypanosoma brucei brucei (strain 427) and promastigotes of Leishmania mexicana mexicana (MHOM/BZ/84/BEL46) were evaluated in vitro. The methanolic root bark extract and standards were profiled by HPLC-PDA, and the majority of compounds identified using literature data. The in vitro antioxidant and anti-inflammatory-like activities were determined by evaluating the effect of crude extracts on reactive oxygen species produced by phorbol 12-myristate 13-acetate-stimulated equine neutrophils using lucigenin-enhanced chemiluminescence and on purified equine myeloperoxidase activity measured by specific immunological extraction followed by enzymatic detection. The methanolic, aqueous crude extract, and aqueous crude extract free of tannins exhibited good growth inhibition on Trypanosoma brucei brucei (IC50 3.72, 6.05, and 4.45 µg/mL respectively) but were inactive against Leishmania mexicana mexicana (IC50 > 100 µg/mL). Suramin (IC50 0.11 µg/mL) and amphotericin (IC50 0.11 µg/mL) were used as standard respectively for the antitrypanosomal and antileishmanial activity. Very interesting antioxidant and anti-inflammatory-like activities were observed with 50% hydroethanolic, aqueous crude extracts, and aqueous crude extract free of tannins as well as with pure punicalagin, gallic, and ellagic acid (IC50 0.38 – 10.51 µg/mL for 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), chemiluminescence, and specific immunological extraction followed by enzymatic detection assays. The study results support traditional medicinal use of the plant for the treatment of parasitical disorders and revealed for the first time the antitrypanosomal potential, anti-inflammatory-like, and antioxidant activity of Terminalia mollis root. [ABSTRACT FROM AUTHOR]

Published
2021
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41. Anti-inflammatory and antioxidant activities of Rungia congoensis, a traditional vegetable consumed by Yombe people from Kongo Central area (DR. Congo)

Author

Mutwale Kapepula, Paulin, primary, Baku Ngumbi, Pacha, additional, Kasongo Kawayidiko, Michel, additional, Duki Mpanzu, Arthur, additional, Franck, Thierry, additional, Mouithys-Mickalad, Ange, additional, Frédérich, Michel, additional, Ngombe Kabamba, Nadege, additional, and Masiala Tsobo, Christophe, additional

Published
2018
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45. Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species

Author

Kapepula, Paulin Mutwale, primary, Kabamba Ngombe, Nadege, additional, Tshisekedi Tshibangu, Pascal, additional, Tsumbu, César, additional, Franck, Thierry, additional, Mouithys-Mickalad, Ange, additional, Mumba, Dieudonné, additional, Tshala-Katumbay, Désiré, additional, Serteyn, Didier, additional, Tits, Monique, additional, Angenot, Luc, additional, Kalenda, Pascal Dibungi T., additional, and Frédérich, Michel, additional

Published
2017
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46. Antiplasmodial activity of Heinsia crinita (Rubiaceae) and identification of new iridoids

Author

Tshisekedi Tshibangu, P., primary, Mutwale Kapepula, P., additional, Kabongo Kapinga, M.J., additional, Tujibikila Mukuta, A., additional, Kalenda, Dibungi T., additional, Tchinda, Alembert T., additional, Mouithys-Mickalad, Ange A., additional, Jansen, O., additional, Cieckiewicz, E., additional, Tits, M., additional, Angenot, L., additional, and Frédérich, M., additional

Published
2017
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47. Antioxidant potentiality of three herbal teas consumed in Bandundu rural areas of Congo

Author

Kapepula, Paulin Mutwale, primary, Mbombo Mungitshi, Patricia, additional, Franck, Thierry, additional, Mouithys-Mickalad, Ange, additional, Mumba Ngoyi, Dieudonné, additional, Kalenda, Pascal Dibungi T., additional, Kabamba Ngombe, Nadege, additional, Serteyn, Didier, additional, Tits, Monique, additional, Frédérich, Michel, additional, and Tamfum Muyembe, Jean-Jacques, additional

Published
2016
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49. Anti-inflammatory and antioxidant activities of Rungia congoensis, a traditional vegetable consumed by Yombe people from Kongo Central area (DR. Congo).

Author

Mutwale Kapepula, Paulin, Baku Ngumbi, Pacha, Kasongo Kawayidiko, Michel, Duki Mpanzu, Arthur, Franck, Thierry, Mouithys-Mickalad, Ange, Frédérich, Michel, Ngombe Kabamba, Nadege, and Masiala Tsobo, Christophe

Subjects
ANTI-inflammatory agents, ANTIOXIDANTS, MYELOPEROXIDASE, LUCIGENIN, YOMBE (Congolese & Angolan people), FLAVONOIDS
Abstract

Rungia congoensis, a traditional vegetable from Kongo Central area (DR. Congo) was studied for establishing microscopic characters and characterised by chromatographic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Microscopically leaf can be characterised by non-glandular and glandular trichomes, sinuous anticlinal epidermal cells, diacytic stomata and helical vessels. Methanolic extract displayed high cellular antioxidant activity at the concentrations range of 0.1–10 μg mL−1 and 1–20 μg mL−1 using lucigenin on neutrophils and DCFH-DA on HL 60, respectively. This extract also showed, more efficient effects on extracellular and intracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of R. congoensis were significantly higher, positively correlated with their phytochemical constituents such as flavonoids, iridoids and phenolic acids; and could justify their use as traditional vegetable and potent local nutraceutical resource. [ABSTRACT FROM AUTHOR]

Published
2019
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50. Phytochemical composition and antioxidant activities of different aerial parts extracts of Ferula communis L.

Author

Rahali, Fatma Zohra, Kefi, Sarra, Bettaieb Rebey, Iness, Hamdaoui, Ghaith, Tabart, Jessica, Kevers, Claire, Franck, Thierry, Mouithys-Mickalad, Ange, and Hamrouni Sellami, Ibtissem

Subjects
PLANT phenols, FERULA, ANTIOXIDANT testing, PHENOLS, OXIDANT status, EXTRACTS
Abstract

The present study aimed to assess antioxidant activities of three organs (flower, fruit, and stem) extracts of Tunisian Ferula (F.) communis. Various experimental models were used to characterize the antioxidant activities in vitro as well as on ROS-induced fluorescence using dichlorofluorescein technique from phorbol myristate acetate (PMA)-stimulated human myeloid cell line HL-60. Results showed that the antioxidant activities varied considerably with organs. Thus, flower exhibited higher DPPH-scavenging ability, reducing and chelating power than stem and fruit. Also, antioxidant capacities using ORAC method and a cell-based assay showed that fruit and stem exhibited statistically similar antioxidant activities. Moreover, F. communis contains high amounts of flavonoids with various health benefits attributed to their antioxidant potential. Likewise, to obtain biologically relevant information, the antioxidant activities of the extracts were evaluated on cellular models implicating the antioxidant activities; this test generally showed that F. communis flower extracts have the highest antioxidant capacities correlated to the highest total phenolic content. The identification of phenolic compounds in F. communis extracts using RP-HPLC revealed that resorcinol, ferulic, and syringic acids together with coumarin were the major molecules. [ABSTRACT FROM AUTHOR]

Published
2019
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