61 results on '"Muñoz, P. Martín"'
Search Results
2. Cooperative insulation of regulatory domains by CTCF-dependent physical insulation and promoter competition
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Thais Ealo, Victor Sanchez-Gaya, Patricia Respuela, María Muñoz-San Martín, Elva Martin-Batista, Endika Haro, and Alvaro Rada-Iglesias
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Science - Abstract
Abstract The specificity of gene expression during development requires the insulation of regulatory domains to avoid inappropriate enhancer-gene interactions. In vertebrates, this insulator function is mostly attributed to clusters of CTCF sites located at topologically associating domain (TAD) boundaries. However, TAD boundaries allow some physical crosstalk across regulatory domains, which is at odds with the specific and precise expression of developmental genes. Here we show that developmental genes and nearby clusters of CTCF sites cooperatively foster the robust insulation of regulatory domains. By genetically dissecting a couple of representative loci in mouse embryonic stem cells, we show that CTCF sites prevent undesirable enhancer-gene contacts (i.e. physical insulation), while developmental genes preferentially contribute to regulatory insulation through non-structural mechanisms involving promoter competition rather than enhancer blocking. Overall, our work provides important insights into the insulation of regulatory domains, which in turn might help interpreting the pathological consequences of certain structural variants.
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- 2024
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3. A Binary Annular Phase Mask to Regulate Spherical Aberration and Allow Super-Localization in Single-Particle Tracking over Extended Depth-of-Focus
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Gresil, Quentin, Lee, Antony, Leveque, Olivier, Caicedo, Karen, Munoz, Blanca Martin, Kulcsar, Caroline, Goudail, Francois, Bon, Pierre, and Cognet, Laurent
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Physics - Optics - Abstract
Important applications of single-particle tracking (SPT) aim at deciphering the diffusion properties of single fluorescent nanoparticles immersed in heterogeneous environments, such as multi-cellular biological tissues. To maximize the particle localization precision in such complex environments, high numerical aperture objectives are often required, which intrinsically restrict depth-of-focus (DOF) to less than a micrometer and impedes recording long trajectories when particles escape the plane of focus. In this work, we show that a simple binary phase mask can work with the spherical aberration inevitably induced by thick sample inhomogeneities, to extend the DOF of a single-molecule fluorescence microscope over more than 4 {\mu}m. The effect of point-spread-function (PSF) engineering over spherical aberration regularizes inhomogeneities of the PSF along the optical axis by restricting it to a narrow distribution. This allows the use of a single fitting function (i.e. Gaussian function) to localize single emitters over the whole extended DOF. Application of this simple approach on diffusing nanoparticles demonstrate that SPT trajectories can be recorded on significantly longer times., Comment: 18 pages, 4 figures
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- 2023
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4. Cooperative insulation of regulatory domains by CTCF-dependent physical insulation and promoter competition
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Ealo, Thais, Sanchez-Gaya, Victor, Respuela, Patricia, Muñoz-San Martín, María, Martin-Batista, Elva, Haro, Endika, and Rada-Iglesias, Alvaro
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- 2024
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5. Untangling the mess of CGRP levels as a migraine biomarker: an in-depth literature review and analysis of our experimental experience
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Gabriel Gárate, Julio Pascual, Marta Pascual-Mato, Jorge Madera, María Muñoz-San Martín, and Vicente González-Quintanilla
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ELISA ,Exercise ,CGRP ,Half-life ,Method ,Migraine ,Medicine - Abstract
Abstract Background Calcitonin gene-related peptide (CGRP) is the most promising candidate to become the first migraine biomarker. However, literature shows clashing results and suggests a methodological source for such discrepancies. We aimed to investigate some of these methodological factors to evaluate the actual role of CGRP as biomarker. Methods Previous to the experimental part, we performed a literature review of articles measuring CGRP in migraine patients. Using our 399 bio-bank sera samples, we performed a series of experiments to test the validity of different ELISA kits employed, time of sample processing, long-term storage, sampling in rest or after moderate exercise. Analysis of in-house data was performed to analyse average levels of the peptide and the effect of sex and age. Results Literature review shows the high variability in terms of study design, determination methods, results and conclusions obtained by studies including CGRP determinations in migraine patients. CGRP measurements depends on the method and specific kit employed, also on the isoform detected, showing completely different ranges of concentrations. Alpha-CGRP and beta-CGRP had median with IQR levels of 37.5 (28.2–54.4) and 4.6 (2.4–6.4)pg/mL, respectively. CGRP content is preserved in serum within the 24 first hours when samples are stored at 4°C after clotting and immediate centrifugation. Storages at -80°C of more than 6 months result in a decrease in CGRP levels. Moderate exercise prior to blood extraction does not modulate the concentration of the peptide. Age positively correlates with beta-CGRP content and men have higher alpha-CGRP levels than women. Conclusions We present valuable information for CGRP measurements in serum. ELISA kit suitability should be tested prior to the experiments. Alpha and beta-CGRP levels should be analysed separately as they can show different behaviours even within the same condition. Samples can be processed in a 24-h window if they have been kept in 4°C and should not be stored for more than 6 months at -80°C before assayed. Patients do not need to rest before the blood extraction unless they have performed a high-endurance exercise. For comparative studies, sex and age should be accounted for as these parameters can impact CGRP concentrations. Graphical Abstract
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- 2024
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6. Active Demand Side Management Operator Tool (Sgclos) and New Communications Architecture in the XXI Century Electrical Grid
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Sánchez, E. García, Aparicio, A. Rodríguez, Casado, M. García, Muñoz, P. Martín, Mora, R., López, A., Román, D., García, M., Sanz, R., Garcia, J. Diaz, Velasco, S. Fresnillo, and Vázquez, A. Valera
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- 2012
7. Inhibition of pro-inflammatory signaling in human primary macrophages by enhancing arginase-2 via target site blockers
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Stephen Fitzsimons, María Muñoz-San Martín, Frances Nally, Eugene Dillon, Ifeolutembi A. Fashina, Moritz J. Strowitzki, Lluís Ramió-Torrentà, Jennifer K. Dowling, Chiara De Santi, and Claire E. McCoy
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MT: Oligonucleotides: Therapies and Applications ,macrophages ,arginase-2 ,miR-155 ,inflammation ,microRNA ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The modulation of macrophage phenotype from a pro-inflammatory to an anti-inflammatory state holds therapeutic potential in the treatment of inflammatory disease. We have previously shown that arginase-2 (Arg2), a mitochondrial enzyme, is a key regulator of the macrophage anti-inflammatory response. Here, we investigate the therapeutic potential of Arg2 enhancement via target site blockers (TSBs) in human macrophages. TSBs are locked nucleic acid antisense oligonucleotides that were specifically designed to protect specific microRNA recognition elements (MREs) in human ARG2 3′ UTR mRNA. TSBs targeting miR-155 (TSB-155) and miR-3202 (TSB-3202) MREs increased ARG2 expression in human monocyte-derived macrophages. This resulted in decreased gene expression and cytokine production of TNF-α and CCL2 and, for TSB-3202, in an increase in the anti-inflammatory macrophage marker, CD206. Proteomic analysis demonstrated that a network of pro-inflammatory responsive proteins was modulated by TSBs. In silico bioinformatic analysis predicted that TSB-3202 suppressed upstream pro-inflammatory regulators including STAT-1 while enhancing anti-inflammatory associated proteins. Proteomic data were validated by confirming increased levels of sequestosome-1 and decreased levels of phosphorylated STAT-1 and STAT-1 upon TSB treatment. In conclusion, upregulation of Arg2 by TSBs inhibits pro-inflammatory signaling and is a promising novel therapeutic strategy to modulate inflammatory signaling in human macrophages.
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- 2023
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8. Co-infection patterns of vector-borne zoonotic pathogens in owned free-ranging dogs in central Chile
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Cevidanes, Aitor, Di Cataldo, Sophia, Muñoz-San Martín, Catalina, Latrofa, Maria Stefania, Hernández, Claudia, Cattan, Pedro E., Otranto, Domenico, and Millán, Javier
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- 2023
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9. High Trypanosoma cruzi prevalence in armadillo (Zaedyus pichiy; Xenarthra: Chlamyphoridae) populations from Mendoza, Argentina
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Morales, Melisa E., Campo Verde Arbocco, Fiorella, Muñoz-San Martín, Catalina, Abba, Agustín M., Ríos, Tatiana A., Cassini, Guillermo H., Cattan, Pedro E., Jahn, Graciela A., and Superina, Mariella
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- 2023
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10. First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
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Muñoz-San Martín, Cristina, Montero-Calle, Ana, Garranzo-Asensio, María, Gamella, Maria, Pérez-Ginés, Víctor, Pedrero, María, Pingarrón, José M., Barderas, Rodrigo, de-los-Santos-Álvarez, Noemí, Lobo-Castañón, María Jesús, and Campuzano, Susana
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- 2023
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11. Anticipating metastasis through electrochemical immunosensing of tumor hypoxia biomarkers
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Muñoz-San Martín, Cristina, Gamella, Maria, Pedrero, María, Montero-Calle, Ana, Pérez-Ginés, Víctor, Camps, Jordi, Arenas, Meritxell, Barderas, Rodrigo, Pingarrón, José M., and Campuzano, Susana
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- 2022
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12. Orphan CpG islands amplify poised enhancer regulatory activity and determine target gene responsiveness
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Pachano, Tomas, Sánchez-Gaya, Víctor, Ealo, Thais, Mariner-Faulí, Maria, Bleckwehl, Tore, Asenjo, Helena G., Respuela, Patricia, Cruz-Molina, Sara, Muñoz-San Martín, María, Haro, Endika, van IJcken, Wilfred F. J., Landeira, David, and Rada-Iglesias, Alvaro
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- 2021
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13. Assessing the viability of electro-absorption and photoelectro-absorption for the treatment of gaseous perchloroethylene
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Muñoz-Morales, Martín, Castañeda-Juárez, Montse, Souza, Fernanda Lourdes, Saez, Cristina, Cañizares, Pablo, Martínez-Miranda, Verónica, Linares-Hernández, Ivonne, and Rodrigo, Manuel Andrés
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- 2021
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14. Gonadotropin-Releasing Hormone Receptor Expression in Human Spinal Cord
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Díaz-Galindo, Carmen, Calderón-Vallejo, Denisse, Hernández-Jasso, Irma, Cervantes-García, Daniel, Martínez-Díaz, Diego, Ibarra-Martínez, David, Muñoz-Ortega, Martín, and Quintanar, J. Luis
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- 2021
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15. Analysis of miRNA signatures in CSF identifies upregulation of miR-21 and miR-146a/b in patients with multiple sclerosis and active lesions
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María Muñoz-San Martín, Gemma Reverter, Rene Robles-Cedeño, Maria Buxò, Francisco José Ortega, Imma Gómez, Jordi Tomàs-Roig, Naiara Celarain, Luisa María Villar, Hector Perkal, José Manuel Fernández-Real, Ester Quintana, and Lluís Ramió-Torrentà
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Active lesions ,Epigenetics ,Gadolinium positive (Gd+) ,Inflammation ,miRNAs ,Multiple sclerosis ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background MicroRNAs (miRNAs) have been reported as deregulated in active brain lesions derived from patients with multiple sclerosis (MS). In there, these post-transcriptional regulators may elicit very important effects but proper identification of miRNA candidates as potential biomarkers and/or therapeutic targets is scarcely available. Objective The aim of the study was to detect the presence of a set of candidate miRNAs in cell-free cerebrospinal fluid (CSF) and to determine their association with gadolinium-enhancing (Gd+) lesions in order to assess their value as biomarkers of MS activity. Methods Assessment of 28 miRNA candidates in cell-free CSF collected from 46 patients with MS (26 Gd+ and 20 Gd− patients) was performed by TaqMan assays and qPCR. Variations in their relative abundance were analyzed by the Mann-Whitney U test and further evaluated by receiver operating characteristic (ROC) analysis. Signaling pathways and biological functions of miRNAs were analyzed using bioinformatic tools (miRTarBase, Enrichr, REVIGO, and Cytoscape softwares). Results Seven out of 28 miRNA candidates were detected in at least 75% of CSF samples. Consistent increase of miR-21 and miR-146a/b was found in Gd+ MS patients. This increase was in parallel to the number of Gd+ lesions and neurofilament light chain (NF-L) levels. Gene Ontology enrichment analysis revealed that the target genes of these miRNAs are involved in biological processes of key relevance such as apoptosis, cell migration and proliferation, and in cytokine-mediated signaling pathways. Conclusion Levels of miR-21 and miR-146a/b in cell-free CSF may represent valuable biomarkers to identify patients with active MS lesions.
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- 2019
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16. Assessing the presence of oligoclonal IgM bands as a prognostic biomarker of cognitive decline in the early stages of multiple sclerosis
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Clàudia Coll‐Martinez, Ester Quintana, Judit Salavedra‐Pont, Maria Buxó, Marina González‐del‐Rio, Immaculada Gómez, María Muñoz‐San Martín, Luisa María Villar, Gary Álvarez‐Bravo, René Robles‐Cedeño, Lluís Ramió‐Torrentà, and Jordi Gich
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biomarkers ,cognitive impairment ,multiple sclerosis ,neuropsychology ,oligoclonal bands ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Abstract Background An association has been found between the presence of lipid‐specific oligoclonal IgM bands (LS‐OCMB) in cerebrospinal fluid and a more severe clinical multiple sclerosis course. Objective To investigate lipid‐specific oligoclonal IgM bands as a prognostic biomarker of cognitive impairment in the early stages of multiple sclerosis. Methods Forty‐four patients underwent neuropsychological assessment at baseline and 4 years. Cognitive performance at follow‐up was compared adjusting by age, education, anxiety–depression, and baseline performance. Results LS‐OCMB+ patients only performed worse for Long‐Term Storage in the Selective Reminding Test (p = .018). Conclusion There are no remarkable cognitive differences between LS‐OCMB– and LS‐OCMB+ patients in the early stages of MS.
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- 2021
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17. A novel peptide-based electrochemical biosensor for the determination of a metastasis-linked protease in pancreatic cancer cells
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Muñoz-San Martín, Cristina, Pedrero, María, Gamella, Maria, Montero-Calle, Ana, Barderas, Rodrigo, Campuzano, Susana, and Pingarrón, José M.
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- 2020
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18. Identification of the Immunological Changes Appearing in the CSF During the Early Immunosenescence Process Occurring in Multiple Sclerosis
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Carmen Picón, Amalia Tejeda-Velarde, José Ignacio Fernández-Velasco, Manuel Comabella, Roberto Álvarez-Lafuente, Ester Quintana, Susana Sainz de la Maza, Enric Monreal, Noelia Villarrubia, José Carlos Álvarez-Cermeño, María Inmaculada Domínguez-Mozo, Lluís Ramió-Torrentà, Eulalia Rodríguez-Martín, Ernesto Roldán, Yolanda Aladro, Silvia Medina, Mercedes Espiño, Jaime Masjuan, Clara Matute-Blanch, Marta Muñoz-San Martín, Carmen Espejo, Carmen Guaza, Alfonso Muriel, Lucienne Costa-Frossard, and Luisa María Villar
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multiple sclerosis ,aging ,innate immunity ,adaptive immunity ,inflammation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Patients with multiple sclerosis (MS) suffer with age an early immunosenescence process, which influence the treatment response and increase the risk of infections. We explored whether lipid-specific oligoclonal IgM bands (LS-OCMB) associated with highly inflammatory MS modify the immunological profile induced by age in MS. This cross-sectional study included 263 MS patients who were classified according to the presence (M+, n=72) and absence (M-, n=191) of LS-OCMB. CSF cellular subsets and molecules implicated in immunosenescence were explored. In M- patients, aging induced remarkable decreases in absolute CSF counts of CD4+ and CD8+ T lymphocytes, including Th1 and Th17 cells, and of B cells, including those secreting TNF-alpha. It also increased serum anti-CMV IgG antibody titers (indicative of immunosenescence) and CSF CHI3L1 levels (related to astrocyte activation). In contrast, M+ patients showed an age-associated increase of TIM-3 (a biomarker of T cell exhaustion) and increased values of CHI3L1, independently of age. Finally, in both groups, age induced an increase in CSF levels of PD-L1 (an inductor of T cell tolerance) and activin A (part of the senescence-associated secretome and related to inflammaging). These changes were independent of the disease duration. Finally, this resulted in augmented disability. In summary, all MS patients experience with age a modest induction of T-cell tolerance and an activation of the innate immunity, resulting in increased disability. Additionally, M- patients show clear decreases in CSF lymphocyte numbers, which could increase the risk of infections. Thus, age and immunological status are important for tailoring effective therapies in MS.
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- 2021
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19. Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study
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Inés Zulantay, Gabriela Muñoz, Daniela Liempi, Tamara Rozas, María José Manneschi, Catalina Muñoz-San Martín, Carezza Botto-Mahan, Werner Apt, and Gonzalo Cabrera
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DTUs ,Trypanosoma cruzi ,real-time PCR ,peripheral blood ,xenodiagnosis ,Triatoma infestans ,Medicine - Abstract
Chagas disease (ChD) is a vector zoonosis native to the American continent caused by the protozoan parasite Trypanosoma cruzi; the biological vectors are multiple species of hematophagous insects of the family Triatominae. A relevant aspect in the host–parasite relationship is the identification of the various genotypes of T. cruzi called discrete typing units (DTU) that circulate in mammals and vectors. In Chile, it has been described that the DTUs TcI, TcII, TcV, and TcVI circulate in infected humans, vectors, and wild animals. Identifying DTUs has acquired clinical importance, since it has been suggested that different genotypes could cause distinct pathologies, circulate in different geographical areas, and present different sensitivities to trypanocidal drugs. In this study, circulating T. cruzi DTUs in peripheral blood and Triatoma infestans dejections used in xenodiagnosis (XD) were amplified by qPCR in 14 Chilean patients with chronic ChD from highly endemic areas. More positive samples were detected by XD compared to peripheral blood samples, and 64.28% of the cases were simple infections and 35.72% mixed, with a statistically significant difference in the frequency of TcV DTU. This study would suggest that T. infestans from Chile is more competent to amplify one DTU over others, probably due to a process of co-evolution.
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- 2022
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20. The Parasite Load of Trypanosoma cruzi Modulates Feeding and Defecation Patterns of the Chagas Disease Vector Triatoma infestans
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Francisco Chacón, Antonella Bacigalupo, Bárbara Álvarez-Duhart, Pedro E. Cattan, Rigoberto Solís, and Catalina Muñoz-San Martín
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Chagas disease ,behavior ,feeding ,defecation ,host–parasite interactions ,insect vector ,Biology (General) ,QH301-705.5 - Abstract
Trypanosoma cruzi is the causal agent of Chagas disease, a parasitic zoonosis transmitted mainly through the feces of triatomine insects. Triatoma infestans is the main triatomine vector of this disease in South America. Previous research has shown that T. cruzi infection modifies the behavior of triatomines. We evaluated, for the first time, the effect of parasite load on feeding and defecation behavior, which we quantified by using real-time PCR. The detection time of the host was shorter in infected individuals, and the number of bites increased, while the dejection time was reduced when compared with the non-infected group. A significant correlation between the parasite load and the behavioral changes registered in the infected triatomines was found. These results would indicate that the intensity of T. cruzi infection modulates the feeding and defecation behavior of T. infestans, increasing the vector competence of this triatomine vector.
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- 2022
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21. Trypanosoma cruzi-infected triatomines and rodents co-occur in a coastal island of northern Chile
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Ricardo Campos-Soto, Gabriel Díaz-Campusano, Nicol Quiroga, Catalina Muñoz-San Martín, Ninette Rives-Blanchard, and Fernando Torres-Pérez
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T. cruzi reservoir in islands ,Island T. cruzi cycle ,Island T. cruzi hosts ,Mepraia ,Insular small mammals ,Hemiptera:Reduviidae ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Trypanosoma cruzi, the cause agent of Chagas disease, is transmitted mainly by blood-feeding insects of the subfamily Triatominae. The T. cruzi life cycle alternates between triatomines and mammalian hosts, excluding birds and reptiles. Triatomines of Mepraia genus are wild vectors of T. cruzi in Chile. Mepraia specimens infected with T. cruzi have been detected in Pan de Azúcar and Santa María islands. The most common vertebrates that inhabit these islands are birds and reptiles, and it is unknown whether small mammals are present. Consequently, it is relevant to know whether there are any T. cruzi-infected small mammals on those islands to elucidate the T. cruzi cycle. To clarify this crossroads, islands of northern Chile were explored to determine if T. cruzi-infected triatomines and rodents co-occur in islands of northern Chile. T. cruzi DNA was detected by conventional and real-time PCR in three islands: on Santa María and Pan de Azúcar islands T. cruzi was detected in Mepraia sp samples, while on Pan de Azúcar (6.1%) and Damas islands (15%) was detected in the rodent Abrothrix olivacea. We show for the first time in Chile the occurrence of insular rodents infected with T. cruzi, and a complete T. cruzi life cycle in a coastal island. Our results provide new insights to understand the T. cruzi infection in the wild cycle.
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- 2020
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22. Trypanosoma cruzi Parasite Load Modulates the Circadian Activity Pattern of Triatoma infestans
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Francisco Chacón, Catalina Muñoz-San Martín, Antonella Bacigalupo, Bárbara Álvarez-Duhart, Rigoberto Solís, and Pedro E. Cattan
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Triatominae ,behavior ,circadian rhythm ,host–parasite interactions ,insect vector ,locomotion ,Science - Abstract
American trypanosomiasis is a disease caused by the flagellate protozoan Trypanosoma cruzi, which is transmitted mainly in endemic areas by blood-sucking triatomine vectors. Triatoma infestans is the most important vector in the southern cone of South America, exhibiting a nocturnal host-seeking behavior. It has been previously documented that the parasite produces changes in some triatomine species, but this is the first time that the behavior of a vector has been evaluated in relation to its parasite load. After comparing the movement events and distance traveled of infected and non-infected T. infestans, we evaluated the change produced by different T. cruzi parasite loads on its circadian locomotor activity. We observed differences between infected and non-infected triatomines, and a significant relation between the parasite load and the increase in locomotor activity of T. infestans, which was accentuated during the photophase. This could have direct implications on the transmission of T. cruzi, as the increased movement and distance traveled could enhance the contact of the vector with the host, while increasing the predation risk for the vector, which could both constitute a risk for vectorial and oral transmission to mammals.
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- 2022
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23. Trypanosoma cruzi load in synanthropic rodents from rural areas in Chile
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Esteban Yefi-Quinteros, Catalina Muñoz-San Martín, Antonella Bacigalupo, Juana P. Correa, and Pedro E. Cattan
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Chagas disease ,Quantitative real-time PCR ,Parasitemia ,Host-parasite relations ,Rattus rattus ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Trypanosoma cruzi is the agent of Chagas disease, a major public health problem in Latin America. Many wild and domestic animals are naturally infected with T. cruzi; rodents are one of the groups which have been consistently detected infected in different countries. The aim of this work was to characterize blood T. cruzi load in naturally infected rodents from a Chagas disease endemic region in Chile. Methods Baited traps were set in domestic and peridomestic areas of rural dwellings. The rodents were anesthetized and blood sampled; DNA was extracted and the parasite load was quantified by T. cruzi satellite DNA real-time PCR assays. Results Seventy-one rodents of four species, Rattus rattus, Mus musculus, Phyllotis darwini and Octodon degus, were captured; R. rattus was the most abundant species. Fifty-nine samples (83.1%) were T. cruzi-positive and the median value of the parasite load was 2.99 parasite equivalents (par-eq)/ml. The comparison of frequency of infection or parasite load by species showed no differences. However, one R. rattus presented very elevated parasitemia (1644 par-eq/ml). Conclusions The overall levels of parasitemia were similar to those found in humans in Chile. The high infection levels in exotic and endemic rodents very near to rural settlements increases their relevance as T. cruzi hosts.
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- 2018
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24. Case report: multiple and atypical amoebic cerebral abscesses resistant to treatment
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Victoria-Hernández, Joaquin Alvaro, Ventura-Saucedo, Anayansi, López-Morones, Aurelio, Martínez-Hernández, Sandra Luz, Medina-Rosales, Marina Nayeli, Muñoz-Ortega, Martín, Ávila-Blanco, Manuel Enrique, Cervantes-García, Daniel, Barba-Gallardo, Luis Fernando, and Ventura-Juárez, Javier
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- 2020
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25. Analysis of miRNA signatures in CSF identifies upregulation of miR-21 and miR-146a/b in patients with multiple sclerosis and active lesions
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Muñoz-San Martín, María, Reverter, Gemma, Robles-Cedeño, Rene, Buxò, Maria, Ortega, Francisco José, Gómez, Imma, Tomàs-Roig, Jordi, Celarain, Naiara, Villar, Luisa María, Perkal, Hector, Fernández-Real, José Manuel, Quintana, Ester, and Ramió-Torrentà, Lluís
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- 2019
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26. Redox Cycling Amplified Electrochemical Lateral-Flow Immunoassay: Toward Decentralized Sensitive Insulin Detection.
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Nandhakumar, Ponnusamy, Muñoz San Martín, Cristina, Arévalo, Beatriz, Ding, Shichao, Lunker, Mahika, Vargas, Eva, Djassemi, Omeed, Campuzano, Susana, and Wang, Joseph
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- 2023
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27. Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases
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Rebeca M. Torrente-Rodríguez, Cristina Muñoz-San Martín, Maria Gamella, María Pedrero, Neus Martínez-Bosch, Pilar Navarro, Pablo García de Frutos, José M. Pingarrón, and Susana Campuzano
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electrochemical immune platform ,human ST2 ,plasma ,pancreatic cancer ,Biotechnology ,TP248.13-248.65 - Abstract
A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL−1) for ST2, allowing the determination of soluble ST2 (sST2) in plasma samples from healthy individuals and patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) in only 45 min once the immunoconjugates have been prepared. The good correlation of the obtained results with those provided by an ELISA kit performed using the same immunoreagents demonstrates the potential of the developed strategy for early diagnosis and/or prognosis of the fatal PDAC disease.
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- 2021
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28. Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases
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María Muñoz-San Martín, Imma Gomez, Albert Miguela, Olga Belchí, René Robles-Cedeño, Ester Quintana, and Lluís Ramió-Torrentà
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CSF ,miRNAs ,neurological diseases ,OpenArray ,Science - Abstract
Background: The study of circulating miRNAs in CSF has gained tremendous attention during the last years, as these molecules might be promising candidates to be used as biomarkers and provide new insights into the disease pathology of neurological disorders. Objective: The main aim of this study was to describe an OpenArray panel of CSF-enriched miRNAs to offer a suitable tool to identify and characterize new molecular signatures in different neurological diseases. Methods: Two hundred and fifteen human miRNAs were selected to be included in the panel, and their expression and abundance in CSF samples were analyzed. In addition, their stability was studied in order to propose suitable endogenous controls for CSF miRNA studies. Results: miR-143-3p and miR-23a-3p were detected in all CSF samples, while another 80 miRNAs were detected in at least 70% of samples. miR-770-5p was the most abundant miRNA in CSF, presenting the lowest mean Cq value. In addition, miR-26b-5p, miR-335-5p and miR-92b-3p were the most stable miRNAs and could be suitable endogenous normalizers for CSF miRNA studies. Conclusions: These OpenArray plates might be a suitable and efficient tool to identify and characterize new molecular signatures in different neurological diseases and would improve the yield of miRNA detection in CSF.
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- 2021
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29. An anti-amoebic vaccine: generation of the recombinant antigen LC3 from Entamoeba histolytica linked to mutated exotoxin A (PEΔIII) via the Pichia pastoris system
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Martínez-Hernández, Sandra Luz, Cervantes-García, Daniel, Muñoz-Ortega, Martín, Aldaba-Muruato, Liseth R., Loera-Muro, Victor M., Ascacio-Martínez, Jorge A., de Jesús Loera-Arias, María, de Oca-Luna, Roberto Montes, and Ventura-Juárez, Javier
- Published
- 2017
- Full Text
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30. During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa
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Bolaños, J.M. Gallardo, Morán, A. Miró, Silva, C.M. Balao da, Dávila, M. Plaza, Muñoz, P. Martín, Aparicio, I.M., Tapia, J.A., Ferrusola, C. Ortega, and Peña, F.J.
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- 2014
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31. Trypanosoma cruzi load in synanthropic rodents from rural areas in Chile
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Yefi-Quinteros, Esteban, Muñoz-San Martín, Catalina, Bacigalupo, Antonella, Correa, Juana P., and Cattan, Pedro E.
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- 2018
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32. Amperometric Immunosensing Scaffolds for Rapid, Simple, Non-Invasive and Accurate Determination of Protein Biomarkers of Well-Accepted and Emerging Clinical Importance
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María Pedrero, Cristina Muñoz-San Martín, Rebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Eva Vargas, F. Javier Manuel de Villena, Rodrigo Barderas, Susana Campuzano, and José M. Pingarrón
- Subjects
n/a ,General Works - Abstract
New amperometric immune-scaffolds for the rapid, sensitive and reliable determination of [...]
- Published
- 2017
- Full Text
- View/download PDF
33. Características de la integración curricular de la informática educativa en el currículo del aula multigrado
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María Angélica Muñoz San Martín and Juan Alberto Sanhueza Vidal
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informática educativa ,currículo ,prácticas pedagógicas ,TIC ,escuela básica rural ,Education (General) ,L7-991 - Abstract
Esta investigación se enmarca en el paradigma cualitativo, posee un carácter descriptivo y relacional y su objetivo fue conocer y describir cómo el profesor integra la informática educativa en el currículum del aula multigrado. Para lograr dicho objetivo, se trabajó con dos Microcentros de la comuna de Temuco y Padre las Casas, con el Facilitador que los asiste y con alumnos de estas organizaciones, las cuales son atendidas técnica y pedagógicamente por la Unidad Ejecutora del Instituto de Informática Educativa del Centro Zonal Sur Austral. En el estudio, se utilizaron dos técnicas de recogida de datos: la entrevista en profundidad y el grupo focal. Este estudio cobra relevancia en el marco del Proyecto Enlaces debido a que su línea Rural, inserta las nuevas tecnologías de la información y comunicación en el aula multigrado para contribuir a la mejora de la calidad y equidad de la educación nacional. Desde esta perspectiva, la investigación es un aporte, considerando que describe cómo el docente rural está integrando la informática en el currículum del aula multigrado, lo que permite elaborar un piso para políticas y estrategias de trabajo pedagógico que tengan relación con la inserción de tecnologías de la información y comunicación en la escuela rural. Finalmente, y como principales conclusiones, se puede informar que los docentes de la muestra están integrando la informática educativa en el currículum y que este proceso se potencia con la motivación que sienten los alumnos por la tarea de aprendizaje al trabajar con el computador. Esto le permite al profesor pasar del ejercicio de una práctica pedagógica tradicional a la de un mediador del aprendizaje a través del desarrollo de actividades colaborativas, por subsectores y por niveles de aprendizaje, asistidas por computador.
- Published
- 2006
34. Adrenergic regulation during acute hepatic infection with Entamoeba histolytica in the hamster: involvement of oxidative stress, Nrf2 and NF-KappaB
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Aldaba-Muruato Liseth Rubi, Muñoz-Ortega Martín Humberto, Macías-Pérez José Roberto, Pulido-Ortega Julieta, Martínez-Hernández Sandra Luz, and Ventura-Juárez Javier
- Subjects
Adrenergic regulation ,oxidative stress ,Nrf2 ,NF-kappa-B ,Entamoeba histolytica ,amebic liver abscess ,Infectious and parasitic diseases ,RC109-216 - Abstract
Oxidative stress and transcriptional pathways of nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa-B (NF-κB) are critically involved in the etiopathology of amebic liver abscess (ALA). In this work, we studied the relationship between the adrenergic nervous system and ALA in the hamster. ALA was visible at 12 h of infection. While 6-hydroxidopamine (6-OHDA) decreased infection, propranolol (β-adrenergic blocker) treatment was associated with less extensive liver damage, and phentolamine treatment (α-adrenergic blocker) significantly reduced ALA compared to 6-OHDA and propranolol. Serum enzymatic activities of alanine aminotransferase (ALT) and γ-glutamyl transpeptidase (γ-GTP) were increased at 12 h post-infection. Chemical denervation and α and β-adrenergic blockers decreased ALT to normal levels, while 6-OHDA and propranolol showed a trend to decrease γ-GTP but phentolamine significantly reduced γ-GTP. Amebic infection increased oxidized glutathione (GSSG) and decreased both reduced glutathione (GSH) and the GSH/GSSG ratio. Propranolol and 6-OHDA showed a tendency to decrease GSSG. However, GSH, GSSG and GSH/GSSG returned to normal levels with phentolamine. Furthermore, amebic infection increased pNF-κB and interleukin-1β (IL-1β), and showed a tendency to decrease hemoxigenase-1 (HO-1), but not Nrf2. Chemical denervation showed a trend to decrease pNF-κB and IL-1β, and neither Nrf2 nor HO-1 increased significantly. In addition, NF-κB and IL-1β were attenuated by propranolol and phentolamine treatments, although phentolamine showed significant overexpression of Nrf2 and HO-1. This suggests that the adrenergic system may be involved in oxidative stress and in modulation of the Nrf2 and NF-κB pathways during ALA development.
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- 2017
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35. Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
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María Pedrero, F. Javier Manuel de Villena, Cristina Muñoz-San Martín, Susana Campuzano, María Garranzo-Asensio, Rodrigo Barderas, and José M. Pingarrón
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human p53 ,magnetic microcarriers ,screen-printed electrodes ,amperometric immunosensor ,cell lysates ,Biotechnology ,TP248.13-248.65 - Abstract
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.
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- 2016
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36. Characterization and evolutionary dynamics of a complex family of satellite DNA in the leaf beetle Chrysolina carnifex (Coleoptera, Chrysomelidae)
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Palomeque, Teresa, Muñoz-López, Martín, Carrillo, José A., and Lorite, Pedro
- Published
- 2005
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37. Coupling of bioleaching and electrokinetic soil flushing for the in-situ removal of impurity from Pb-Zn mine tailings.
- Author
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Acosta Hernández, Irene, Muñoz Morales, Martín, López-Bellido Garrido, Francisco Javier, Rodríguez, Luis, and Villaseñor Camacho, José
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BACTERIAL leaching ,SOIL washing ,METAL tailings ,RESISTANCE heating ,ENERGY dissipation ,ABANDONED mines ,ELECTRIC currents - Abstract
This study combines bioleaching and electrokinetics (EK) for the in-situ removal of metals from polluted mine tailings collected from an abandoned Pb-Zn mine. The proposed treatment strategy consisted in the external production of an acid bioleaching (BL) medium in a bioreactor by means of autochthonous acidophilic microorganisms incubated from real mine tailings; next, the BL medium was subsequently used as anolyte and placed in contact in situ with the polluted soil, taking advantage of EK mechanisms r for dissolution and transport of metals to the cathode compartment. After obtaining a microbial culture capable of producing the bioleaching medium, lab-scale EK experiments were performed, and the influence of the voltage dosage (0.5, 1.0 and 1.5 V cm
−1 ) was assessed. The results obtained showed that the BL medium improved the metal removal efficiency, and that the applied voltage gradient had a clear influence. Specifically, the combination of an electric current under 1.5 V cm−1 and the BL medium increased the metal removal rates by 17 % (Fe), 4 % (Cu), 2 % (Zn), 12 % (Mn), 12 % (Ni), 1 % (Cr) and 7 % (Co), as compared to those without using BL. With this novel treatment, it was also possible to comply with the local regulatory requirements for most of the metals studied. This technology offers several advantages over other EK-bioleaching configurations previously reported. It was found that the limiting factor in the process was the voltage gradient applied, as higher voltages increased metal removal yields but also producing higher soil heating with the consequent energy losses. [Display omitted] • A new bio-electrokinetic method for in situ metals removal from tailings was tested. • Externally produced bioleaching medium was used as anolyte in electrokinetic tests. • The bioleaching medium enhanced in situ metal solubilization in tailings. • Higher voltages increased metal removal yields towards the cathode compartment. • Under 1.5 Vcm−1 ohmic heating in tailings and thus energy losses were observed. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
38. Modification of the Daily Activity Pattern of the Diurnal Triatomine Mepraia spinolai(Hemiptera: Reduviidae) Induced by Trypanosoma cruzi(Trypanosomatida: Trypanosomatidae) Infection
- Author
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Pérez, G, Muñoz-San Martín, C, Chacón, F, Bacigalupo, A, Cattan, P E, and Solís, R
- Abstract
Mepraia spinolai,(Porter) 1934, is a diurnal triatomine endemic to Chile and a wild vector of the protozoan Trypanosoma cruzi,(Chagas) 1909, which causes Chagas disease. Behavioral changes in M. spinolaiinduced by this parasite have been reported previously, which include detection of a potential host, defecation latency, and some life history traits. In this study we assessed changes in locomotor and daily activity due to infection with T. cruzi. No difference was detected in distance traveled between infected and uninfected individuals. However, the groups differed in their daily activity patterns; infected individuals showed significant reduction of movements during the light phase and concentrated their activity in the dark phase. Uninfected individuals showed no differences in locomotor activity between the phases. The results suggest that T. cruziinduces a displacement in the activity of M. spinolaitoward the dark phase of the circadian cycle, which may improve its vector competence.
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- 2021
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39. LAS GRIETAS DEL CORAZÓN: HALLAZGO DE DOS COMPLICACIONES MECÁNICAS MORTALES
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Callen, Alberto Garrido, Muñoz, Irene Fernández, and Muñoz, Marta Martín
- Published
- 2024
- Full Text
- View/download PDF
40. Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats.
- Author
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Peugnet-González, Ivonne, Luz Martínez-Hernández, Sandra, Enrique Ávila-Blanco, Manuel, Alejandro Hernández-Marín, David, Roberto Macias-Pérez, José, Rubí Aldaba-Muruato, Liseth, Quezada-Tristán, Teódulo, Sosa-Ramírez, Joaquín, Stephanie Villa-Jaimes, Gloria, Ventura-Juárez, Javier, Muñoz-Ortega, Martín, and Ibarra-Martínez, David
- Abstract
[Display omitted] • Watercress extract could prevent the progression of liver fibrosis. • Watercress extract restores the levels of NRF2 in the liver and the overexpression of IL-10 in both the liver and intestine. • Watercress extract reduced the hepatic expression of the fibrogenic factor TGF-β and the pro-inflammatory cytokine IL-1. • Watercress extract has the potential to serve as a natural therapeutic alternative for liver fibrosis. The present study aimed to analyze the antioxidant and hepatoprotective efficacy of watercress extract against liver fibrosis induced by CCl 4 (0.8 mL/kg, i.p) in an in vivo rat model. Four groups were included, each with five animals (n = 5): 1) Intact, 2) CCl 4 -induced for 4 weeks, 3) CCl 4 -induced for 8 weeks, and 4) CCl 4 -induced for 4 weeks followed by watercress extract treatment (600 mg/kg/day) until week 8. Tissue samples were analyzed using histological techniques (H&E, Sirius Red, Masson's trichrome stain), biochemical methods, and gene expression analysis of IL-1, IL-10, TGF-β, NRF2, Keap1, Acta2, Timp-2, MMP-13, and MMP-9. Watercress extract administration resulted in improvements by preventing steatosis and fibrosis. It also increased IL-10 expression in the liver and intestine. Additionally, the expression of fibrogenic markers such as TGF-β, Col 1α, Acta2, and Timp-2 was regulated. These results suggest that orally administered watercress extract prevents the progression of liver fibrosis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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41. Calidad de vida de las personas con úlceras por presión. Estudio cualitativo fenomenológico.
- Author
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MUÑOZ, Begoña MARTÍN
- Published
- 2018
42. The decline of infant and child mortality among Spanish Gitanos or Calé (1871-2005): A microdemographic study in Andalusia.
- Author
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Gamella, Juan F. and Carrasco-Muñoz, Elisa Martín
- Subjects
INFANT mortality ,ROMANIES ,GOVERNMENT policy ,DEMOGRAPHIC surveys - Abstract
BACKGROUND Most Romani groups in Europe have experienced a decline in childhood mortality during recent decades. These crucial transformations are rarely addressed in research or public policy. OBJECTIVE This paper analyzes the timing and structure of the decline of childhood mortality among the Gitano people of Spain. METHODS The paper is based on the family and genealogy reconstitution of the Gitano population of 22 contiguous localities in Southern Spain. Registry data from over 19,100 people and 3,501 reconstituted families was included in a dense genealogical grid ranging over 150 years. From this database we produced annual time series of infant and child mortality and of the registered causes of death from 1871 to 2005. RESULTS The analyzed data shows a rapid decline in infant and child mortality from about 1949 to 1970. The onset of the definitive decline occurred in the late 1940s and early 1950s. Child mortality was higher in the pre-transitional period and started to decline earlier, although it took longer to converge with majority rates. The mortality transition in the Gitano minority paralleled that of the dominant majority, but with important delays and higher mortality rates. The causes of death show the deprivation suffered by Gitano people. CONCLUSIONS The childhood mortality decline facilitated the most important changes experienced recently by the Gitano minority, including its fertility transition and the transformation of Gitanos' gender and family systems. CONTRIBUTION This is one of the first historical reconstructions of the mortality transition of a Romani population. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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43. Stoma care nurses' perspectives on the relative significance of factors influencing ostomates' quality of life
- Author
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Díaz, Concepción Capilla, Zambrano, Sandra Milena Hernández, Muñoz, Begoña Martín, Crisol, Inmaculada Sánchez, Marfil, Maria Nieves Pérez, Montoya-Juárez, Rafael, and Montoro, César Hueso
- Abstract
Aims/objectives:To analyse the relevance in clinical practice of factors reviewed in previous studies that define the experience of people with a stoma (ostomates), through the opinions of stoma care nurses.Method:Categories and thematic statements were reviewed by stoma care nurses using a modified Delphi method. They assessed the clinical relevance of the previously identified issues by measuring the intensity of the described situations within their practice.Results:Social impact was the most significant issue. Other relevant aspects were ‘description of disease’, ‘interaction with mutual help groups’, ‘health education’ and ‘consultation with professionals to improve self-care’, among others. In view of these results, an explanatory model was proposed whose central category was the ‘necessity of educating ostomates’, assuming that, if it is mediated by stoma care nurses in combination with others (such as mutual help groups), it will have a positive impact on the promotion of self-care, and it would reduce the negative impact of the stoma.Conclusions:The social dimension was the most outstanding area in the care of ostomates. Patients were afraid of social relationships, triggering situations of isolation and limiting their exposure to unfamiliar environments. If precautions related to stoma care are maintained, it will allow them to live a life without these limitations.
- Published
- 2018
- Full Text
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44. Caracterización y optimización de amplificadores de microondas mediante la técnica de las funciones descriptivas
- Author
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Muñoz San Martín, Sagrario and Muñoz San Martín, Sagrario
- Abstract
En este trabajo se aplica la técnica de las funciones descriptivas al análisis de circuitos no-lineales en régimen de gran seal como son los amplificadores de rf. Aunque esta técnica ya se ha aplicado a dispositivos activos de una puerta, (osciladores), la extensión de su empleo a dispositivos no-lineales multipuerta, (bit, mesfet y hemt), no se habia desarrollado debido en parte a la dificultad para obtener las propias funciones descriptivas, (admitancias dinámicas del dispositivo), y en parte a la gran complejidad matemática que supone la resolución simultanea del conjunto de ecuaciones no-lineales que representa al sistema. La determinación de las funciones descriptivas implica el desarrollo de un modelo de parámetros concentrados para el elemento activo. En este estudio se ha desarrollado un modelo preciso para mesfet, incluyendo incluso la dependencia con la polarización, y se ha reducido la complejidad matemática mediante hipótesis previas sobre las formas de onda y estados de operación del transistor que posteriormente han sido plenamente justificadas. La comprobación de los resultados obtenidos por la técnica de las funciones descriptivas se lleva a cabo mediante técnicas de simulación bien probadas y por los resultados experimentales obtenidos de los distintos amplificadores diseñados. La excelente concordancia demuestra que si bien es necesario a priori un conocimiento de las formas de onda, conocimiento obtenido a partir de medidas experimentales o del análisis de la acción de filtrado de las redes lineales del circuito, la aplicación de esta técnica a redes no-lineales multipuerta permite un análisis completo y general de este tipo de circuitos. El método propuesto permite sin excesiva complejidad matemática, una caracterización completa del amplificador incluyendo las formas de onda de las tensiones y corrientes, la síntesis de las impedancias de entrada y salida y el calculo de los principales parámetros característicos. Los resultados obtenidos confir
- Published
- 1996
45. New methodological approaches in the anthropological demography of Romani groups. An example from the study of the evolution of the infant and child mortality of the Gitanos or Calé of Spain (1871-2007).
- Author
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Gamella, Juan F., Carrasco-Muñoz, Elisa Martín, and Quesada Garrido, Alejandro
- Abstract
Copyright of Sociologia (08723419) is the property of Universidade do Porto, Faculdade de Letras and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2014
46. Proceedings of the Frontiers of Retrovirology Conference 2016
- Author
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Zurnic, Irena, Hütter, Sylvia, Lehmann, Ute, Stanke, Nicole, Reh, Juliane, Kern, Tobias, Lindel, Fabian, Gerresheim, Gesche, Hamann, Martin, Müllers, Erik, Lesbats, Paul, Cherepanov, Peter, Serrao, Erik, Engelman, Alan, Lindemann, Dirk, Da Silva Santos, Claire, Tartour, Kevin, Cimarelli, Andrea, Burdick, Rya, Chen, Jianbo, Sastri, Jaya, Hu, Wei-Shau, Pathak, Vinay, Keppler, Oliver T., Pradeau, Karine, Eiler, Sylvia, Levy, Nicolas, Lennon, Sarah, Cianferani, Sarah, Emiliani, Stéphane, Ruff, Marc, Parissi, Vincent, Rato, Sylvie, Rausell, Antonio, Munoz, Miguel, Telenti, Amalio, Ciuffi, Angela, Zhyvoloup, Alexander, Melamed, Anat, Anderson, Ian, Planas, Delphine, Kriston-Vizi, Janos, Ketteler, Robin, Lee, Chen- Hsuin, Merritt, Andy, Ancuta, Petronela, Bangham, Charles, Fassati, Ariberto, Rodari, Anthony, Van Driessche, Benoit, Galais, Mathilde, Delacourt, Nadége, Fauquenoy, Sylvain, Vanhulle, Caroline, Kula, Anna, Burny, Arsène, Rohr, Olivier, Van Lint, Carine, van Montfort, Thijs, van der Sluis, Renee, Speijer, Dave, Berkhout, Ben, Meng, Bo, Rutkowski, Andrzej, Berry, Neil, Dölken, Lars, Lever, Andrew, Schuster, Thomas, Asbach, Benedikt, Wagner, Ralf, Gross, Christine, Wiesmann, Veit, Kalmer, Martina, Wittenberg, Thomas, Gettemans, Jan, Thoma-Kress, Andrea K., Li, Minghua, Freed, Eric O., Liu, Shan-Lu, Müller, Janis, Münch, Jan, Sewald, Xaver, Uchil, Pradeep, Ladinsky, Mark, Beloor, Jagadish, Pi, Ruoxi, Herrmann, Christin, Motamedi, Nasim, Murooka, Thomas, Brehm, Michael, Greiner, Dale, Mempel, Thorsten, Bjorkman, Pamela, Kumar, Priti, Mothes, Walther, Joas, Simone, Parrish, Erica, Gnanadurai, Clement Wesley, Lump, Edina, Stürzel, Christina M., Parrish, Nicholas F., Sauermann, Ulrike, Töpfer, Katharina, Schultheiss, Tina, Bosinger, Steven, Silvestri, Guido, Apetrei, Cristian, Huot, Nicholas, Müller-Trutwin, Michaela, Sauter, Daniel, Hahn, Beatrice H., Stahl-Hennig, Christiane, Kirchhoff, Frank, Schumann, Gerald, Jung-Klawitter, Sabine, Fuchs, Nina V., Upton, Kyle R., Muñoz-Lopez, Martin, Shukla, Ruchi, Wang, Jichang, Garcia-Canadas, Marta, Lopez-Ruiz, Cesar, Gerhardt, Daniel J., Sebe, Attila, Grabundzija, Ivana, Gerdes, Patricia, Merkert, Sylvia, Pulgarin, Andres, Bock, Anja, Held, Ulrike, Witthuhn, Anett, Haase, Alexandra, Wolvetang, Ernst J., Martin, Ulrich, Ivics, Zoltán, Izsvák, Zsuzsanna, Garcia-Perez, J., Faulkner, Geoffrey J., Hurst, Tara, Katzourakis, Aris, Magiorkinis, Gkikas, Schott, Kerstin, Derua, Rita, Seifried, Janna, Reuter, Andreas, Schmitz, Heike, Tondera, Christiane, Brandariz-Nuñez, Alberto, Diaz-Griffero, Felipe, Janssens, Veerle, König, Renate, Baldauf, Hanna-Mari, Stegmann, Lena, Schwarz, Sarah-Marie, Trotard, Maud, Martin, Margarethe, Lenzi, Gina, Burggraf, Manja, Pan, Xiaoyu, Fregoso, Oliver I., Lim, Efrem S., Abraham, Libin, Erikson, Elina, Nguyen, Laura, Ambiel, Ina, Rutsch, Frank, Kim, Baek, Emerman, Michael, Fackler, Oliver T., Wittmann, Sabine, Behrendt, Rayk, Volkmann, Bianca, Eissmann, Kristin, Gramberg, Thomas, Bolduan, Sebastian, Koppensteiner, Herwig, Regensburg, Stefanie, Brack-Werner, Ruth, Draenert, Rika, Schindler, Michael, Ducroux, Aurélie, Xu, Shuting, Ponnurangam, Aparna, Franz, Sergej, Malassa, Angelina, Ewald, Ellen, Goffinet, Christine, Fung, Sin-Yee, Chan, Ching-Ping, Yuen, Chun-Kit, Kok, Kin-Hang, Chan, Chin-Ping, Jin, Dong-Yan, Dittmer, Ulf, Kmiec, Dorota, Iyer, Shilpa, Stürzel, Christina, Hahn, Beatrice, Ariumi, Yasuo, Yasuda-Inoue, Mariko, Kawano, Koudai, Tateishi, Satoshi, Turelli, Priscilla, Compton, Alex, Roy, Nicolas, Porrot, Françoise, Billet, Anne, Casartelli, Nicoletta, Yount, Jacob, Liang, Chen, Schwartz, Oliver, Magnus, Carsten, Reh, Lucia, Moore, Penny, Uhr, Therese, Weber, Jacqueline, Morris, Lynn, Trkola, Alexandra, Grindberg, Rashel V., Schlaepfer, Erika, Schreiber, Gideon, Simon, Viviana, Speck, Roberto F., Debyser, Zeger, Vranckx, Lenard, Demeulemeester, Jonas, Saleh, Suha, Verdin, Eric, Cereseto, Anna, Christ, Frauke, Gijsbers, Rik, Wang, Gang, Zhao, Na, Das, Atze T., Köstler, Josef, Perdiguero, Beatriz, Esteban, Mariano, Jacobs, Bertram L., Montefiori, David C., LaBranche, Celia C., Yates, Nicole L., Tomaras, Georgia D., Ferrari, Guido, Foulds, Kathryn E., Roederer, Mario, Landucci, Gary, Forthal, Donald N., Seaman, Michael S., Hawkins, Natalie, Self, Steven G., Phogat, Sanjay, Tartaglia, James, Barnett, Susan W., Burke, Brian, Cristillo, Anthony D., Ding, Song, Heeney, Jonathan L., Pantaleo, Giuseppe, Stab, Viktoria, Ensser, Armin, Tippler, Bettina, Burton, Dennis, Tenbusch, Matthias, Überla, Klaus, Alter, Galit, Lofano, Giuseppe, Dugast, Anne-Sophie, Kulkarni, Viraj, Suscovich, Todd, Opazo, Tatiana, Barraza, Felipe, Herrera, Diego, Garces, Andrea, Schwenke, Tomas, Tapia, Diego, Cancino, Jorge, Arriagada, Gloria, Haußner, Christina, Damm, Dominik, Rohrhofer, Anette, Schmidt, Barbara, Eichler, Jutta, Midgley, Rebecca, Wheeldon, James, Piguet, Vincent, Khopkar, Priyanka, Rohamare, Megha, Kulkarni, Smita, Godinho-Santos, Ana, Hance, Allan, Goncalves, Joao, Mammano, Fabrizio, Gasser, Romain, Hamoudi, Meriem, Pellicciotta, Martina, Zhou, Zhicheng, Visdeloup, Clara, Colin, Philippe, Braibant, Martine, Lagane, Bernard, Negroni, Matteo, Wamara, Jula, Bannert, Norbert, Mesplede, Thibault, Osman, Nathan, Anstett, Kaitlin, Liang, Jiaming Calvin, Pham, Hanh Thi, Wainberg, Mark, Shao, Wei, Shan, Jigui, Kearney, Mary, Wu, Xiaolin, Maldarelli, Frank, Mellors, John, Luke, Brian, Coffin, John, Hughes, Stephen, Fricke, Thomas, Opp, Silvana, Shepard, Caitlin, Ivanov, Dmitri, Valle-Casuso, Jose, Kanja, Marine, Cappy, Pierre, Lener, Daniela, Knyazhanskaya, Ekaterina, Anisenko, Andrey, Zatsepin, Timofey, Gottikh, Marina, Komkov, Alexander, Minervina, Anastasia, Nugmanov, Gaiaz, Nazarov, Vadim, Khodosevich, Konstantin, Mamedov, Ilgar, Lebedev, Yuri, Colomer-Lluch, Marta, Serra-Moreno, Ruth, Sarracino, Ambra, Gharu, Lavina, Pasternak, Alexander, Marcello, Alessandro, McCartin, Ann Marie, Kulkarni, Anurag, Le Douce, Valentin, Gautier, Virginie, Baeyens, Ann, Naessens, Evelien, Van Nuffel, Anouk, Weening, Karin, Reilly, Anne- Marie, Claeys, Eva, Trypsteen, Wim, Vandekerckhove, Linos, Eyckerman, Sven, Gevaert, Kris, Verhasselt, Bruno, Mok, Hoi Ping, Norton, Nicholas, Fun, Axel, Hirst, Jack, Wills, Mark, Miklik, Dalibor, Senigl, Filip, Hejnar, Jiri, Sakuragi, Jun-ichi, Sakuragi, Sayuri, Yokoyama, Masaru, Shioda, Tatsuo, Sato, Hironori, Bodem, Jochen, Moschall, Rebecca, Denk, Sarah, Erkelenz, Steffen, Schenk, Christian, Schaal, Heiner, Donhauser, Norbert, Socher, Ellen, Millen, Sebastian, Sticht, Heinrich, Mann, Melanie, Wei, Guochao, Betts, Matthew J., Liu, Yang, Kehl, Timo, Russell, Robert B., Löchelt, Martin, Hohn, Oliver, Mostafa, Saeed, Hanke, Kirsten, Norley, Stephen, Chen, Chia-Yen, Shingai, Masashi, Borrego, Pedro, Taveira, Nuno, Strebel, Klaus, Hellmund, Chris, Friedrich, Melanie, Hahn, Friedrich, Setz, Christian, Rauch, Pia, Fraedrich, Kirsten, Matthaei, Alina, Henklein, Petra, Traxdorf, Maximilian, Fossen, Torgils, Schubert, Ulrich, Khwaja, Aya, Galilee, Meytal, Alian, Akram, Schwalbe, Birco, Hauser, Heiko, Schreiber, Michael, Scherpenisse, Mirte, Cho, Young-Keol, Kim, Jungeun, Jeong, Daeun, Trejbalova, Katerina, Benesova, Martina, Kucerova, Dana, Vernerova, Zdenka, Amouroux, Rachel, Hajkova, Petra, Elleder, Daniel, Hron, Tomas, Farkasova, Helena, Padhi, Abinash, Paces, Jan, Zhu, Henan, Gifford, Robert, Murcia, Pablo, Carrozza, Maria Luisa, Niewiadomska, Anna-Maria, Mazzei, Maurizio, Abi-Said, Mounir, Hughes, Joseph, Hué, Stéphane, Obasa, Adetayo, Jacobs, Graeme, Engelbrecht, Susan, Mack, Katharina, Starz, Kathrin, Geyer, Matthias, Bibollet-Ruche, Frederic, Leoz, Marie, Plantier, Jean Christophe, Argaw-Denboba, Ayele, Balestrieri, Emanuela, Serafino, Annalucia, Bucci, Ilaria, Cipriani, Chiara, Spadafora, Corrado, Sinibaldi-Vallebona, Paolo, Matteucci, Claudia, Jayashree, S. Nandi, Neogi, Ujjwal, Chhangani, Anil K., Rathore, Shravan Sing, Mathur, Bajrang R. J., Abati, Adeyemi, Koç, B. Taylan, Oğuzoğlu, Tuba Çiğdem, Shimauchi, Takatoshi, Caucheteux, Stephan, Turpin, Jocelyn, Finsterbusch, Katja, Tokura, Yoshiki, Souriant, Shanti, Balboa, Luciana, Pingris, Karine, Kviatcowsky, Denise, Raynaud-Messina, Brigitte, Cougoule, Céline, Mercier, Ingrid, Kuroda, Marcelo, González-Montaner, Pablo, Inwentarz, Sandra, Moraña, Eduardo Jose, del Carmen Sasiain, Maria, Neyrolles, Olivier, Maridonneau-Parini, Isabelle, Lugo-Villarino, Geanncarlo, Vérollet, Christel, Herrmann, Alexandra, Thomas, Dominique, Bouzas, Nerea Ferreirós, Lahaye, Xavier, Bhargava, Anvita, Satoh, Takeshi, Gentili, Matteo, Cerboni, Silvia, Silvin, Aymeric, Conrad, Cécile, Ahmed-Belkacem, Hakim, Rodriguez, Elisa C., Guichou, Jean-François, Bosquet, Nathalie, Piel, Matthieu, Le Grand, Roger, King, Megan, Pawlotsky, Jean-Michel, Manel, Nicolas, Hofmann, Henning, Vanwalscappel, Benedicte, Bloch, Nicolin, Landau, Nathaniel, Indik, Stanislav, Hagen, Benedikt, Valle-Casuso, José Carlos, Allouch, Awatef, David, Annie, Barré-Sinoussi, Françoise, Benkirane, Monsef, Pancino, Gianfranco, Saez-Cirion, Asier, Lee, Wing-Yiu, Sloan, Richard, Schulte, Bianca, Blomberg, Jonas, Vargiu, Luana, Rodriguez-Tomé, Patricia, Tramontano, Enzo, Sperber, Göran, Kumari, Namita, Ammosova, Tatiana, Diaz, Sharmeen, Oneal, Patricia, Nekhai, Sergei, Fahrny, Audrey, Gers-Huber, Gustavo, Audigé, Annette, Jayaprakash, Anitha, Sachidanandam, Ravi, Hernandez, Matt, Dillon-White, Marsha, Maze, Emmanuel, Ham, Claire, Almond, Neil, Towers, Greg, Belshaw, Robert, de Sousa-Pereira, Patrícia, Abrantes, Joana, Pizzato, Massimo, Esteves, Pedro J., Kahle, Tanja, Schmitt, Sven, Merkel, Laura, Reuter, Nina, Stamminger, Thomas, Rosa, Ilaria Dalla, Bishop, Kate, Spinazzola, Antonella, Groom, Harriet, Vieyres, Gabrielle, Müsken, Mathias, Zillinger, Thomas, Hornung, Veit, Barchet, Winfried, Häussler, Susanne, Pietschmann, Thomas, Javed, Aneela, Leuchte, Nicole, Salinas, Gabriela, Opitz, Lennart, Sopper, Sieghart, Mummert, Christiane, Hofmann, Christian, Hückelhoven, Angela G., Bergmann, Silke, Müller-Schmucker, Sandra M., Harrer, Ellen G., Dörrie, Jan, Schaft, Niels, Harrer, Thomas, Cardinaux, Laure, Zahno, M.- L., Vogt, H.- R., Zanoni, R., Bertoni, G., Muenchhoff, Maximilian, Goulder, Philip, Keppler, Oliver, Rebensburg, Stephanie, Helfer, Markus, Zhang, Yuwei, Chen, Huicheng, Bernier, Annie, Gosselin, Annie, Routy, Jean- Pierre, Wöhrl, Birgitta, Schneider, Anna, Corona, Angela, Spöring, Imke, Jordan, Mareike, Buchholz, Bernd, Maccioni, Elias, Di Santo, Roberto, Schweimer, Kristian, Schölz, Christian, Weinert, Brian, Wagner, Sebastian, Beli, Petra, Miyake, Yasuyuki, Qi, Jun, Jensen, Lars, Streicher, Werner, McCarthy, Anna, Westwood, Nicholas, Lain, Sonia, Cox, Jürgen, Matthias, Patrick, Mann, Matthias, Bradner, James, Choudhary, Chunaram, Stern, Marcel, Valletta, Elena, Frezza, Caterina, Marino-Merlo, Francesca, Grelli, Sandro, Serafino, Anna Lucia, Mastino, Antonio, Macchi, Beatrice, Kaulfuß, Meike, Windmann, Sonja, Bayer, Wibke, Mikasi, Sello, Heß, Rebecca, Bonsmann, Michael Storcksdieck gen., Kirschning, Carsten, Lepenies, Bernd, Kolenbrander, Anne, Temchura, Vladimir, Iijima, Kenta, Kobayashi, Junya, and Ishizaka, Yukihito
- Abstract
Table of contents Oral presentations Session 1: Entry & uncoating O1 Host cell polo-like kinases (PLKs) promote early prototype foamy virus (PFV) replication Irena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Reh, Tobias Kern, Fabian Lindel, Gesche Gerresheim, Martin Hamann, Erik Müllers, Paul Lesbats, Peter Cherepanov, Erik Serrao, Alan Engelman, Dirk Lindemann O2 A novel entry/uncoating assay reveals the presence of at least two species of viral capsids during synchronized HIV-1 infection Claire Da Silva Santos, Kevin Tartour, Andrea Cimarelli O3 Dynamics of nuclear envelope association and nuclear import of HIV-1 complexes Rya Burdick, Jianbo Chen, Jaya Sastri, Wei-Shau Hu, Vinay Pathak O4 Human papillomavirus protein E4 potently enhances the susceptibility to HIV infection Oliver T. Keppler Session 2: Reverse transcription & integration O5 Structure and function of HIV-1 integrase post translational modifications Karine Pradeau, Sylvia Eiler, Nicolas Levy, Sarah Lennon, Sarah Cianferani, Stéphane Emiliani, Marc Ruff O6 Regulation of retroviral integration by RNA polymerase II associated factors and chromatin structure Vincent Parissi Session 3: Transcription and latency O7 A novel single-cell analysis pipeline to identify specific biomarkers of HIV permissiveness Sylvie Rato, Antonio Rausell, Miguel Munoz, Amalio Telenti, Angela Ciuffi O8 A capsid-dependent integration program linking T cell activation to HIV-1 gene expression Alexander Zhyvoloup, Anat Melamed, Ian Anderson, Delphine Planas, Janos Kriston-Vizi, Robin Ketteler, Chen-Hsuin Lee, Andy Merritt, Petronela Ancuta, Charles Bangham, Ariberto Fassati O9 Characterisation of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome Anthony Rodari, Benoit Van Driessche, Mathilde Galais, Nadége Delacourt, Sylvain Fauquenoy, Caroline Vanhulle, Anna Kula, Arsène Burny, Olivier Rohr, Carine Van Lint O10 Tissue-specific dendritic cells differentially modulate latent HIV-1 reservoirs Thijs van Montfort, Renee van der Sluis, Dave Speijer, Ben Berkhout Session 4: RNA trafficking & packaging O11 A novel cis-acting element affecting HIV replication Bo Meng, Andrzej Rutkowski, Neil Berry, Lars Dölken, Andrew Lever O12 Tolerance of HIV’s late gene expression towards stepwise codon adaptation Thomas Schuster, Benedikt Asbach, Ralf Wagner Session 5: Assembly & release O13 Importance of the tax-inducible actin-bundling protein fascin for transmission of human T cell leukemia virus Type 1 (HTLV-1) Christine Gross, Veit Wiesmann, Martina Kalmer, Thomas Wittenberg, Jan Gettemans, Andrea K. Thoma-Kress O14 Lentiviral nef proteins antagonize TIM-mediated inhibition of viral release Minghua Li, Eric O. Freed, Shan-Lu Liu Session 6: Pathogenesis & evolution O15 SEVI and semen prolong the half-life of HIV-1 Janis Müller, Jan Münch O16 CD169+ macrophages mediate retrovirus trans-infection of permissive lymphocytes to establish infection in vivo Xaver Sewald, Pradeep Uchil, Mark Ladinsky, Jagadish Beloor, Ruoxi Pi, Christin Herrmann, Nasim Motamedi, Thomas Murooka, Michael Brehm, Dale Greiner, Thorsten Mempel, Pamela Bjorkman, Priti Kumar, Walther Mothes O17 Efficient replication of a vpu containing SIVagm construct in African Green Monkeys requires an HIV-1 nef gene Simone Joas, Erica Parrish, Clement Wesley Gnanadurai, Edina Lump, Christina M. Stürzel, Nicholas F. Parrish, Ulrike Sauermann, Katharina Töpfer, Tina Schultheiss, Steven Bosinger, Guido Silvestri, Cristian Apetrei, Nicholas Huot, Michaela Müller-Trutwin, Daniel Sauter, Beatrice H. Hahn, Christiane Stahl-Hennig, Frank Kirchhoff O18 Reprogramming initiates mobilization of endogenous mutagenic LINE-1, Alu and SVA retrotransposons in human induced pluripotent stem cells with consequences for host gene expression Gerald Schumann, Sabine Jung-Klawitter, Nina V. Fuchs, Kyle R. Upton, Martin Muñoz-Lopez, Ruchi Shukla, Jichang Wang, Marta Garcia-Canadas, Cesar Lopez-Ruiz, Daniel J. Gerhardt, Attila Sebe, Ivana Grabundzija, Patricia Gerdes, Sylvia Merkert, Andres Pulgarin, Anja Bock, Ulrike Held, Anett Witthuhn, Alexandra Haase, Ernst J. Wolvetang, Ulrich Martin, Zoltán Ivics, Zsuzsanna Izsvák, J. Garcia-Perez, Geoffrey J. Faulkner O19 NF-κB activation induces expression of human endogenous retrovirus and particle production Tara Hurst, Aris Katzourakis, Gkikas Magiorkinis Session 7a and b: Innate sensing & intrinsic immunity O20 Identification of the phosphatase acting on T592 in SAMHD1 during M/G1 transition Kerstin Schott, Rita Derua, Janna Seifried, Andreas Reuter, Heike Schmitz, Christiane Tondera, Alberto Brandariz-Nuñez, Felipe Diaz-Griffero, Veerle Janssens, Renate König O21 Vpx overcomes a SAMHD1-independent block to HIV reverse transcription that is specific to resting CD4 T cells Hanna-Mari Baldauf, Lena Stegmann, Sarah-Marie Schwarz, Maud Trotard, Margarethe Martin, Gina Lenzi, Manja Burggraf, Xiaoyu Pan, Oliver I. Fregoso, Efrem S. Lim, Libin Abraham, Elina Erikson, Laura Nguyen, Ina Ambiel, Frank Rutsch, Renate König, Baek Kim, Michael Emerman, Oliver T. Fackler, Oliver T. Keppler O22 The role of SAMHD1 in antiviral restriction and immune sensing in the mouse Sabine Wittmann, Rayk Behrendt, Bianca Volkmann, Kristin Eissmann, Thomas Gramberg O23 T cells expressing reduced restriction factors are preferentially infected in therapy naïve HIV-1 patients Sebastian Bolduan, Herwig Koppensteiner, Stefanie Regensburg, Ruth Brack-Werner, Rika Draenert, Michael Schindler O24 cGAS-mediated innate immunity spreads through HIV-1 env-induced membrane fusion sites from infected to uninfected primary HIV-1 target cells Aurélie Ducroux, Shuting Xu, Aparna Ponnurangam, Sergej Franz, Angelina Malassa, Ellen Ewald, Christine Goffinet O25 Perturbation of innate RNA and DNA sensing by human T cell leukemia virus type 1 oncoproteins Sin-Yee Fung, Ching-Ping Chan, Chun-Kit Yuen, Kin-Hang Kok, Chin-Ping Chan, Dong-Yan Jin O26 Induction and anti-viral activity of Interferon α subtypes in HIV-1 infection Ulf Dittmer O27 Vpu-mediated counteraction of tetherin is a major determinant of HIV-1 interferon resistance Dorota Kmiec, Shilpa Iyer, Christina Stürzel, Daniel Sauter, Beatrice Hahn, Frank Kirchhoff O28 DNA repair protein Rad18 restricts HIV-1 and LINE-1 life cycle Yasuo Ariumi, Mariko Yasuda-Inoue, Koudai Kawano, Satoshi Tateishi, Priscilla Turelli O29 Natural mutations in IFITM3 allow escape from post-translational regulation and toggle antiviral specificity Alex Compton, Nicolas Roy, Françoise Porrot, Anne Billet, Nicoletta Casartelli, Jacob Yount, Chen Liang, Oliver Schwartz Session 8: Adaptive immunity & immune evasion O30 Observing evolution in HIV-1 infection: phylogenetics and mutant selection windows to infer the influence of the autologous antibody response on the viral quasispecies Carsten Magnus, Lucia Reh, Penny Moore, Therese Uhr, Jacqueline Weber, Lynn Morris, Alexandra Trkola O31 Dose and subtype specific analyses of the anti-HIV effects of IFN-alpha family members Rashel V. Grindberg, Erika Schlaepfer, Gideon Schreiber, Viviana Simon, Roberto F. Speck Session 9: Novel antiviral strategies O32 LEDGIN-mediated inhibition of the integrase-LEDGF/p75 interaction reduces reactivation of residual latent HIV Zeger Debyser, Lenard Vranckx, Jonas Demeulemeester, Suha Saleh, Eric Verdin, Anna Cereseto, Frauke Christ, Rik Gijsbers O33 NKG2D-mediated clearance of reactivated viral reservoirs by natural killer cells O34 Inhibition of HIV reactivation in brain cells by AAV-mediated delivery of CRISPR/Cas9 O35 CRISPR-Cas9 as antiviral: potent HIV-1 inhibition, but rapid virus escape and the subsequent design of escape-proof antiviral strategies Ben Berkhout, Gang Wang, Na Zhao, Atze T. Das Session 10: Recent advances in HIV vaccine development O36 Priming with a potent HIV-1 DNA vaccine frames the quality of T cell and antibody responses prior to a poxvirus and protein boost Benedikt Asbach, Josef Köstler, Beatriz Perdiguero, Mariano Esteban, Bertram L. Jacobs, David C. Montefiori, Celia C. LaBranche, Nicole L. Yates, Georgia D. Tomaras, Guido Ferrari, Kathryn E. Foulds, Mario Roederer, Gary Landucci, Donald N. Forthal, Michael S. Seaman, Natalie Hawkins, Steven G. Self, Sanjay Phogat, James Tartaglia, Susan W. Barnett, Brian Burke, Anthony D. Cristillo, Song Ding, Jonathan L. Heeney, Giuseppe Pantaleo, Ralf Wagner O37 Passive immunisation with a neutralising antibody against HIV-1 Env prevents infection of the first cells in a mucosal challenge rhesus monkey model Christiane Stahl-Hennig, Viktoria Stab, Armin Ensser, Ulrike Sauermann, Bettina Tippler, Dennis Burton, Matthias Tenbusch, Klaus Überla O38 HIV antibody Fc-glycoforms drive B cell affinity maturation Galit Alter, Giuseppe Lofano, Anne-Sophie Dugast, Viraj Kulkarni, Todd Suscovich Poster presentations Topic 1: Entry & uncoating P1 Dynein light chain is required for murine leukemia virus infection Tatiana Opazo, Felipe Barraza, Diego Herrera, Andrea Garces, Tomas Schwenke, Diego Tapia, Jorge Cancino, Gloria Arriagada P2 Peptide paratope mimics of the broadly neutralising HIV-1 antibody b12 Christina Haußner, Dominik Damm, Anette Rohrhofer, Barbara Schmidt, Jutta Eichler P3 Investigating cellular pathways involved in the transmission of HIV-1 between dendritic cells and T cells using RNAi screening techniques Rebecca Midgley, James Wheeldon, Vincent Piguet P4 Co-receptor tropism in HIV-1, HIV-2 monotypic and dual infections Priyanka Khopkar, Megha Rohamare, Smita Kulkarni P5 Characterisation of the role of CIB1 and CIB2 as HIV-1 helper factors Ana Godinho-Santos, Allan Hance, Joao Goncalves, Fabrizio Mammano P6 Buffering deleterious polymorphisms in the highly constrained C2 region of HIV-1 envelope by the flexible V3 domain Romain Gasser, Meriem Hamoudi, Martina Pellicciotta, Zhicheng Zhou, Clara Visdeloup, Philippe Colin, Martine Braibant, Bernard Lagane, Matteo Negroni P7 Entry inhibition of HERV-K(HML-2) by an Env-IgG fusion protein Jula Wamara, Norbert Bannert Topic 2: Reverse transcription & integration P8 The R263K/H51Y resistance substitutions in HIV integrase decreases levels of integrated HIV DNA over time Thibault Mesplede, Nathan Osman, Kaitlin Anstett, Jiaming Calvin Liang, Hanh Thi Pham, Mark Wainberg P9 The Retrovirus Integration Database (RID) Wei Shao, Jigui Shan, Mary Kearney, Xiaolin Wu, Frank Maldarelli, John Mellors, Brian Luke, John Coffin, Stephen Hughes P10 The small molecule 3G11 inhibits HIV-1 reverse transcription Thomas Fricke, Silvana Opp, Caitlin Shepard, Dmitri Ivanov, Baek Kim, Jose Valle-Casuso, Felipe Diaz-Griffero P11 Dual and opposite regulation of HIV-1 integration by hRAD51: impact on therapeutical approaches using homologous DNA repair modulators Vincent Parissi P12 A flexible motif essential for integration by HIV-1 integrase Marine Kanja, Pierre Cappy, Matteo Negroni, Daniela Lener P13 Interaction between HIV-1 integrase and the host protein Ku70: identification of the binding site and study of the influence on integrase-proteasome interplay Ekaterina Knyazhanskaya, Andrey Anisenko, Timofey Zatsepin, Marina Gottikh P14 Normalisation based method for deep sequencing of somatic retroelement integrations in human genome Alexander Komkov, Anastasia Minervina, Gaiaz Nugmanov, Vadim Nazarov, Konstantin Khodosevich, Ilgar Mamedov, Yuri Lebedev Topic 3: Transcription and latency P15 BCA2/RABRING7 restricts HIV-1 transcription by preventing the nuclear translocation of NF-κB Marta Colomer-Lluch, Ruth Serra-Moreno P16 MATR3 post-transcriptional regulation of HIV-1 transcription during latency Ambra Sarracino, Anna Kula, Lavina Gharu, Alexander Pasternak, Carine Van Lint, Alessandro Marcello P17 HIV-1 tat intersects the SUMO pathway to regulate HIV-1 promoter activity Ann Marie McCartin, Anurag Kulkarni, Valentin Le Douce, Virginie Gautier P18 Conservation in HIV-1 Vpr guides tertiary gRNA folding and alternative splicing Ann Baeyens, Evelien Naessens, Anouk Van Nuffel, Karin Weening, Anne-Marie Reilly, Eva Claeys, Wim Trypsteen, Linos Vandekerckhove, Sven Eyckerman, Kris Gevaert, Bruno Verhasselt P19 The majority of reactivatable latent HIV are genetically distinct Hoi Ping Mok, Nicholas Norton, Axel Fun, Jack Hirst, Mark Wills, Andrew Lever P20 Do mutations in the tat exonic splice enhancer contribute to HIV-1 latency? Nicholas Norton, Hoi Ping Mok, Jack Hirst, Andrew Lever P21 Culture-to-Ct: A fast and direct RT-qPCR HIV gene reactivation screening method using primary T cell culture Valentin Le Douce, Ann Marie McCartin, Virginie Gautier P22 A novel approach to define populations of early silenced proviruses Dalibor Miklik, Filip Senigl, Jiri Hejnar Topic 4: RNA trafficking & packaging P23 Functional analysis of the structure and conformation of HIV-1 genome RNA DIS Jun-ichi Sakuragi, Sayuri Sakuragi, Masaru Yokoyama, Tatsuo Shioda, Hironori Sato P24 Regulation of foamy viral env splicing controls gag and pol expression Jochen Bodem, Rebecca Moschall, Sarah Denk, Steffen Erkelenz, Christian Schenk, Heiner Schaal Topic 5: Assembly & release P25 Transfer of HTLV-1 p8 to target T cells depends on VASP: a novel interaction partner of p8 Norbert Donhauser, Ellen Socher, Sebastian Millen, Heinrich Sticht, Andrea K. Thoma-Kress P26 COL4A1 and COL4A2 are novel HTLV-1 tax targets with a putative role in virus transmission Christine Gross, Sebastian Millen, Melanie Mann, Klaus Überla, Andrea K. Thoma-Kress P27 The C terminus of foamy virus gag protein is required for particle formation, and virus budding: starting assembly at the C terminus? Guochao Wei, Matthew J. Betts, Yang Liu, Timo Kehl, Robert B. Russell, Martin Löchelt P28 Generation of an antigen-capture ELISA and analysis of Rec and Staufen-1 effects on HERV-K(HML-2) virus particle production Oliver Hohn, Saeed Mostafa, Kirsten Hanke, Stephen Norley, Norbert Bannert P29 Antagonism of BST-2/tetherin is a conserved function of primary HIV-2 Env glycoproteins Chia-Yen Chen, Masashi Shingai, Pedro Borrego, Nuno Taveira, Klaus Strebel P30 Mutations in the packaging signal region of the HIV-1 genome cause a late domain mutant phenotype Chris Hellmund, Bo Meng, Andrew Lever P31 p6 regulates membrane association of HIV-1 gag Melanie Friedrich, Friedrich Hahn, Christian Setz, Pia Rauch, Kirsten Fraedrich, Alina Matthaei, Petra Henklein, Maximilian Traxdorf, Torgils Fossen, Ulrich Schubert Topic 6: Pathogenesis & evolution P32 Molecular and structural basis of protein evolution during viral adaptation Aya Khwaja, Meytal Galilee, Akram Alian P33 HIV-1 enhancement and neutralisation by soluble gp120 and its role for the selection of the R5-tropic “best fit” Birco Schwalbe, Heiko Hauser, Michael Schreiber P34 An insertion of seven amino acids in the Env cytoplasmic tail of Human Immunodeficiency Virus type 2 (HIV-2) selected during disease progression enhances viral replication François Dufrasne, Mara Lucchetti, Patrick Goubau, Jean Ruelle P35 Cell-associated HIV-1 unspliced to multiply spliced RNA ratio at 12 weeks ART correlates with markers of immune activation and apoptosis and predicts the CD4 T-cell count at 96 weeks ART Mirte Scherpenisse, Ben Berkhout, Alexander Pasternak P36 Faster progression in non-B subtype HIV-1-infected patients than Korean subclade of subtype B is accompanied by higher variation and no induction of gross deletion in non-B nef gene by Korean red ginseng treatment Young-Keol Cho, Jungeun Kim, Daeun Jeong P37 Aberrant expression of ERVWE1 endogenous retrovirus and overexpression of TET dioxygenases are characteristic features of seminoma Katerina Trejbalova, Martina Benesova, Dana Kucerova, Zdenka Vernerova, Rachel Amouroux, Petra Hajkova, Jiri Hejnar P38 Life history of the oldest lentivirus: characterisation of ELVgv integrations and the TRIM5 selection pattern in dermoptera Daniel Elleder, Tomas Hron, Helena Farkasova, Abinash Padhi, Jan Paces P39 Characterisation of a highly divergent endogenous retrovirus in the equine germ line Henan Zhu, Robert Gifford, Pablo Murcia P40 The emergence of pandemic retroviral infection in small ruminants Maria Luisa Carrozza, Anna-Maria Niewiadomska, Maurizio Mazzei, Mounir Abi-Said, Joseph Hughes, Stéphane Hué, Robert Gifford P41 Near full-length genome (NFLG) Characterisation of HIV-1 subtype B identified in South Africa Adetayo Obasa, Graeme Jacobs, Susan Engelbrecht P42 Acquisition of Vpu-mediated tetherin antagonism by an HIV-1 group O strain Katharina Mack, Kathrin Starz, Daniel Sauter, Matthias Geyer, Frederic Bibollet-Ruche, Christina Stürzel, Marie Leoz, Jean Christophe Plantier, Beatrice H. Hahn, Frank Kirchhoff P43 The human endogenous retrovirus type K is involved in cancer stem cell markers expression and in human melanoma malignancy Ayele Argaw-Denboba, Emanuela Balestrieri, Annalucia Serafino, Ilaria Bucci, Chiara Cipriani, Corrado Spadafora, Paolo Sinibaldi-Vallebona, Claudia Matteucci P44 Natural infection of Indian non-human primates by unique lentiviruses S. Nandi Jayashree, Ujjwal Neogi, Anil K. Chhangani, Shravan Sing Rathore, Bajrang R. J. Mathur P45 Free cervical cancer screening among HIV-positive women receiving antiretroviral treatment in Nigeria Adeyemi Abati P46 Molecular evolutionary status of feline immunodeficiency virus in Turkey B. Taylan Koç, Tuba Çiğdem Oğuzoğlu Topic 7: Innate sensing & intrinsic immunity P47 Cell-to-cell contact with HTLV-1-infected T cells reduces dendritic cell immune functions and contributes to infection in trans. Takatoshi Shimauchi, Stephan Caucheteux, Jocelyn Turpin, Katja Finsterbusch, Charles Bangham, Yoshiki Tokura, Vincent Piguet P48 Deciphering the mechanisms of HIV-1 exacerbation induced by Mycobacterium tuberculosis in monocytes/macrophages Shanti Souriant, Luciana Balboa, Karine Pingris, Denise Kviatcowsky, Brigitte Raynaud-Messina, Céline Cougoule, Ingrid Mercier, Marcelo Kuroda, Pablo González-Montaner, Sandra Inwentarz, Eduardo Jose Moraña, Maria del Carmen Sasiain, Olivier Neyrolles, Isabelle Maridonneau-Parini, Geanncarlo Lugo-Villarino, Christel Vérollet P49 The SAMHD1-mediated inhibition of LINE-1 retroelements is regulated by phosphorylation Alexandra Herrmann, Sabine Wittmann, Caitlin Shepard, Dominique Thomas, Nerea Ferreirós Bouzas, Baek Kim, Thomas Gramberg P50 Activities of nuclear envelope protein SUN2 in HIV infection Xavier Lahaye, Anvita Bhargava, Takeshi Satoh, Matteo Gentili, Silvia Cerboni, Aymeric Silvin, Cécile Conrad, Hakim Ahmed-Belkacem, Elisa C. Rodriguez, Jean-François Guichou, Nathalie Bosquet, Matthieu Piel, Roger Le Grand, Megan King, Jean-Michel Pawlotsky, Nicolas Manel P51 Activation of TLR7/8 with a small molecule agonist induces a novel restriction to HIV-1 infection of monocytes Henning Hofmann, Benedicte Vanwalscappel, Nicolin Bloch, Nathaniel Landau P52 Steady state between the DNA polymerase and Rnase H domain activities of reverse transcriptases determines the sensitivity of retroviruses to inhibition by APOBEC3 proteins Stanislav Indik, Benedikt Hagen P53 HIV restriction in mature dendritic cells is related to p21 induction and p21-mediated control of the dNTP pool and SAMHD1 activity. José Carlos Valle-Casuso, Awatef Allouch, Annie David, Françoise Barré-Sinoussi, Michaela Müller-Trutwin, Monsef Benkirane, Gianfranco Pancino, Asier Saez-Cirion P54 IFITM protens restrict HIV-1 protein synthesis Wing-Yiu Lee, Chen Liang, Richard Sloan P55 Characterisation and functional analysis of the novel restriction factor Serinc5 Bianca Schulte, Silvana Opp, Felipe Diaz-Griffero P56 piRNA sequences are common in Human Endogenous Retroviral Sequences (HERVs): An antiretroviral restriction mechanism? Jonas Blomberg, Luana Vargiu, Patricia Rodriguez-Tomé, Enzo Tramontano, Göran Sperber P57 Ferroportin restricts HIV-1 infection in sickle cell disease Namita Kumari, Tatiana Ammosova, Sharmeen Diaz, Patricia Oneal, Sergei Nekhai P58 APOBEC3G modulates the response to antiretroviral drugs in humanized mice Audrey Fahrny, Gustavo Gers-Huber, Annette Audigé, Roberto F. Speck, Anitha Jayaprakash, Ravi Sachidanandam, Matt Hernandez, Marsha Dillon-White, Viviana Simon P59 High-throughput epigenetic analysis of evolutionarily young endogenous retrovirus presents in the mule deer (Odocoileus hemionus) genome Tomas Hron, Helena Farkasova, Daniel Elleder P60 Characterisation of the expression of novel endogenous retroviruses and immune interactions in a macaque model Neil Berry, Emmanuel Maze, Claire Ham, Neil Almond, Greg Towers, Robert Belshaw P61 HIV-1 restriction by orthologs of SERINC3 and SERINC5 Patrícia de Sousa-Pereira, Joana Abrantes, Massimo Pizzato, Pedro J. Esteves, Oliver T. Fackler, Oliver T. Keppler, Hanna-Mari Baldauf P62 TRIM19/PML restricts HIV infection in a cell type-dependent manner Bianca Volkmann, Tanja Kahle, Kristin Eissmann, Alexandra Herrmann, Sven Schmitt, Sabine Wittmann, Laura Merkel, Nina Reuter, Thomas Stamminger, Thomas Gramberg P63 Recent invasion of the mule deer genome by a retrovirus Helena Farkasova, Tomas Hron, Daniel Elleder P64 Does the antiviral protein SAMHD1 influence mitochondrial function? Ilaria Dalla Rosa, Kate Bishop, Antonella Spinazzola, Harriet Groom P65 cGAMP transfers intercellularly via HIV-1 Env-mediated cell–cell fusion sites and triggers an innate immune response in primary target cells Shuting Xu, Aurélie Ducroux, Aparna Ponnurangam, Sergej Franz, Gabrielle Vieyres, Mathias Müsken, Thomas Zillinger, Angelina Malassa, Ellen Ewald, Veit Hornung, Winfried Barchet, Susanne Häussler, Thomas Pietschmann, Christine Goffinet P66 Pre-infection transcript levels of FAM26F in PBMCS inform about overall plasma viral load in acute and postacute phase after SIV-infection Ulrike Sauermann, Aneela Javed, Nicole Leuchte, Gabriela Salinas, Lennart Opitz, Christiane Stahl-Hennig, Sieghart Sopper P67 Sequence-function analysis of three T cell receptors targeting the HIV-1 p17 epitope SLYNTVATL Christiane Mummert, Christian Hofmann, Angela G. Hückelhoven, Silke Bergmann, Sandra M. Müller-Schmucker, Ellen G. Harrer, Jan Dörrie, Niels Schaft, Thomas Harrer P68 An immunodominant region of the envelope glycoprotein of small ruminant lentiviruses may function as decoy antigen Laure Cardinaux, M.-L. Zahno, H.-R. Vogt, R. Zanoni, G. Bertoni P69 Impact of immune activation, immune exhaustion, broadly neutralising antibodies and viral reservoirs on disease progression in HIV-infected children Maximilian Muenchhoff, Philip Goulder, Oliver Keppler Topic 9: Novel antiviral strategies P70 Identification of natural compounds as new antiviral products by bioassay-guided fractionation Alexandra Herrmann, Stephanie Rebensburg, Markus Helfer, Michael Schindler, Ruth Brack-Werner P71 The PPARG antagonism disconnects the HIV replication and effector functions in Th17 cells Yuwei Zhang, Huicheng Chen, Delphine Planas, Annie Bernier, Annie Gosselin, Jean-Pierre Routy, Petronela Ancuta P72 Characterisation of a multiresistant subtype AG reverse transcriptase: AZT resistance, sensitivity to RNase H inhibitors and inhibitor binding Birgitta Wöhrl, Anna Schneider, Angela Corona, Imke Spöring, Mareike Jordan, Bernd Buchholz, Elias Maccioni, Roberto Di Santo, Jochen Bodem, Enzo Tramontano, Kristian Schweimer P73 Insigths into the acetylation pattern of HDAC inhibitors and their potential role in HIV therapy Christian Schölz, Brian Weinert, Sebastian Wagner, Petra Beli, Yasuyuki Miyake, Jun Qi, Lars Jensen, Werner Streicher, Anna McCarthy, Nicholas Westwood, Sonia Lain, Jürgen Cox, Patrick Matthias, Matthias Mann, James Bradner, Chunaram Choudhary P74 HPV-derived and seminal amyloid peptides enhance HIV-1 infection and impair the efficacy of broadly neutralising antibodies and antiretroviral drugs Marcel Stern, Oliver T. Keppler P75 D(−)lentiginosine inhibits both proliferation and virus expression in cells infected by HTLV-1 in vitro Elena Valletta, Caterina Frezza, Claudia Matteucci, Francesca Marino-Merlo, Sandro Grelli, Anna Lucia Serafino, Antonio Mastino, Beatrice Macchi P76 HIV-1 resistance analyses of the Cape Winelands districts, South Africa Sello Mikasi, Graeme Jacobs, Susan Engelbrecht Topic 10: Recent advances in HIV vaccine development P77 Induction of complex retrovirus antigen-specific immune responses by adenovirus-based vectors depends on the order of vector administration Meike Kaulfuß, Sonja Windmann, Wibke Bayer P78 Direct impact of structural properties of HIV-1 Env on the regulation of the humoral immune response Rebecca Heß, Michael Storcksdieck gen. Bonsmann, Viktoria Stab, Carsten Kirschning, Bernd Lepenies, Matthias Tenbusch, Klaus Überla P79 Lentiviral virus-like particles mediate gerenration of T-follicular helper cells in vitro Anne Kolenbrander, Klaus Überla, Vladimir Temchura P80 Recruitment of HIV-1 Vpr to DNA damage sites and protection of proviral DNA from nuclease activity Kenta Iijima, Junya Kobayashi, Yukihito Ishizaka
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- 2016
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47. From the Euromed to the Union for the Mediterranean: Challenges and Answers.
- Author
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Muñoz, Gema Martín
- Subjects
INTERNATIONAL cooperation ,EUROPEAN foreign relations ,INTERNATIONAL relations - Abstract
Copyright of Hellenic Studies / Etudes Helleniques is the property of Centre for Hellenic Studies & Research Canada-KEEK and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2009
48. EL PROYECTO EUROMEDITERRÁNEO, ESPEJO DE LAS CONTRADICCIONES EUROPEAS.
- Author
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Muñoz, Gema Martín
- Abstract
In 2005, ten years after the start of the Euro-Mediterranean process of regional partnership, little progress had been made toward the goals set forth in the Barcelona Declaration. The declaration was a significant achievement because it approached relations between the European Union and south Mediterranean countries from a global, not simply economic, perspective. The agreements set forth in the Barcelona Declaration reflected the idea that the best way to stabilize a world region was not only to support liberal economic reform but also to help in the development of civil societies and to facilitate cultural meeting points.
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- 2006
49. Enrichment of Human ESC‐Derived Multipotent Mesenchymal Stem Cells with Immunosuppressive and Anti‐Inflammatory Properties Capable to Protect Against Experimental Inflammatory Bowel Disease
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Sánchez, Laura, Gutierrez‐Aranda, Iván, Ligero, Gertrudis, Rubio, Ruth, Muñoz‐López, Martín, García‐Pérez, José L., Ramos, Verónica, Real, Pedro. J., Bueno, Clara, Rodríguez, René, Delgado, Mario, and Menendez, Pablo
- Abstract
Human ESCs provide access to the earliest stages of human development and may serve as an unlimited source of functional cells for future cell therapies. The optimization of methods directing the differentiation of human embryonic stem cells (hESCs) into tissue‐specific precursors becomes crucial. We report an efficient enrichment of mesenchymal stem cells (MSCs) from hESCs through specific inhibition of SMAD‐2/3 signaling. Human ESC‐derived MSCs (hESC‐MSCs) emerged as a population of fibroblastoid cells expressing a MSC phenotype: CD73+ CD90+ CD105+ CD44+ CD166+ CD45− CD34− CD14− CD19− human leucocyte antigen‐DR (HLA‐DR)−. After 28 days of SMAD‐2/3 inhibition, hESC cultures were enriched (>42%) in multipotent MSCs. CD73+CD90+ hESC‐MSCs were fluorescence activated cell sorting (FACS)‐isolated and long‐term cultures were established and maintained for many passages displaying a faster growth than somatic tissue‐derived MSCs while maintaining MSC morphology and phenotype. They displayed osteogenic, adipogenic, and chondrocytic differentiation potential and exhibited potent immunosuppressive and anti‐inflammatory properties in vitro and in vivo, where hESC‐MSCs were capable of protecting against an experimental model of inflammatory bowel disease. Interestingly, the efficient enrichment of hESCs into MSCs through inhibition of SMAD‐2/3 signaling was not reproducible with distinct induced pluripotent stem cell lines. Our findings provide mechanistic insights into the differentiation of hESCs into immunosuppressive and anti‐inflammatory multipotent MSCs with potential future clinical applications. STEMCELLS2011;29:251–262
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- 2011
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50. Growth hormone ameliorates high glucose-induced steatosis on in vitro cultured human HepG2 hepatocytes by inhibiting de novo lipogenesis via ChREBP and FAS suppression.
- Author
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Villanueva-Ortega, Eréndira, Méndez-García, Lucia A., Garibay-Nieto, Guadalupe N., Laresgoiti-Servitje, Estibalitz, Medina-Bravo, Patricia, Olivos-García, Alfonso, Muñoz-Ortega, Martín H., Ventura-Juárez, Javier, and Escobedo, Galileo
- Abstract
Objective : Growth hormone (GH) deficiency has been associated with increased steatosis but the molecular mechanism has not been fully elucidated. We investigated the effect of GH on lipid accumulation of HepG2 cells cultured on an in vitro steatosis model and examined the potential involvement of insulin-like growth factor 1 (IGF-1) as well as lipogenic and lipolytic molecules. Methods : Control and steatosis conditions were induced by culturing HepG2 cells with 5.5 or 25 mmol/l glucose for 24 h, respectively. Afterward, cells were exposed to 0, 5, 10 or 20 ng/ml GH for another 24 h. Lipid content was quantified as well as mRNA and protein levels of IGF-1, carbohydrate responsive element-binding protein (ChREBP), sterol regulatory element-binding protein 1c (SREBP1c), fatty acid synthase (FAS), carnitine palmitoyltransferase 1A (CPT1A), and peroxisome proliferator-activated receptor alpha (PPAR-alpha) by qPCR and western blot, respectively. Data were analyzed by one-way ANOVA and the Games-Howell post-hoc test. Results : In the steatosis model, HepG2 hepatocytes showed a significant 2-fold increase in lipid amount as compared to control cells. IGF-1 mRNA and protein levels were significantly increased in control cells exposed to 10 ng/ml GH, whereas high glucose abolished this effect. High glucose also significantly increased both mRNA and protein of ChREBP and FAS without having effect on SREBP1c, CPT1A and PPAR-alpha. However, GH inhibited ChREBP and FAS production, even in HepG2 hepatocytes cultured under steatosis conditions. Conclusions : Growth hormone ameliorates high glucose-induced steatosis in HepG2 cells by suppressing de novo lipogenesis via ChREBP and FAS down-regulation. • Growth hormone directly suppresses lipogenic gene expression in HepG2 cells. • Growth hormone does not affect lypolitic gene expression in HepG2 cells. • HepG2 cells cultured with 25 mmol/l glucose display in vitro steatosis. • High glucose concentrations suppress IGF-1 expression in HepG2 cells. • Growth hormone ameliorates high glucose-induced steatosis in HepG2 cells. [ABSTRACT FROM AUTHOR]
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- 2020
- Full Text
- View/download PDF
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