1. Hydrogel assisted synthesis of gold nanoparticles with enhanced microbicidal and in vivo wound healing potential
- Author
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Zahra Batool, Gulzar Muhammad, Muhammad Mudassir Iqbal, Muhammad Shahbaz Aslam, Muhammad Arshad Raza, Noreen Sajjad, Muhammad Abdullah, Naeem Akhtar, Asad Syed, Abdallah M. Elgorban, Salim S. Al-Rejaie, and Zahid Shafiq
- Subjects
Medicine ,Science - Abstract
Abstract The present study reports a hydrogel-based sunlight-assisted synthesis of gold nanoparticles (Au NPs) with enhanced antimicrobial and wound healing potential. The hydrogel extracted from the seeds of Cydonia oblonga was used as a reducing and capping agent to synthesize Au NPs for the first time. The as-synthesized Au NPs were characterized for an average size, shape, surface functionalization, antimicrobial, and wound healing capabilities. The cubic and rectangular-shaped Au NPs with an average edge length of 74 ± 4.57 nm depicted a characteristic surface plasmon resonance band at 560 nm. The hydrogel-based Au NPs inhibited the growth of microorganisms in zones with 12 mm diameter. In-vitro experiments showed that a minimum inhibitory concentration of Au NPs (16 µg/mL) was sufficient to mimic the 95% growth of pathogenic microorganisms in 24 h. In vivo treatment of wounds with Au NPs in murine models revealed a 99% wound closure within 5 days. Quantitative PCR analysis performed to decipher the role of Au NPs in enhanced wound healing showed an increase in the expression levels of NANOG and CD-34 proteins.
- Published
- 2022
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