Search

Abstract Present study assessed the growth of two plant species and ion uptake by them grown on different proportion of industrial solid waste and garden soil. The industrial waste having high concentration of chemicals were used with garden soil at different proportion i.e. 0% (T0), 5% (T1), 10% (T2), 15% (T3) and 20% (T4). Two species namely Conocarpus erectus (alien plant) and Dodonaea viscosa (indigenous) were used as test plants in pot study. Different parameters including growth, physiology, and anatomy of plants and concentration of cations (Na+, K+, Ca2+, and Mg2+) in the plant shoot and root were measured at different time duration (initial, 1st, 2nd, 3rd and 4th month). The key objective of the study was to use these plants to establish their plantations on the barren lands where industrial solid wastes were being disposed of. C. erectus showed better growth than D. viscosa, as well as more uptake of ions. A significant increase in plant growth was observed in fourth month in T1, where plant height reached 24.5% and 46% for C. erectus and D. viscosa, respectively. At harvest, in C. erectus, no significant difference in the fresh (65–78 g) and dry weight (24–30 g) of the shoot was observed across treatments compared to the control. In D. viscosa, at the time of harvest, the fresh and dry weights of the root and shoot showed a strong, significantly decreasing pattern across T1, T2, and T3, leading to the death of the plant at T3 and T4. Further, optimum ratio of waste soil to garden soil was found as 10:90 and 20:80 to establish the plantations of D. viscosa and C. erectus, respectively in areas where such solid waste from industries are disposed. Findings can be used for the restoration of such solid waste for the sustainable management of industrial areas and their associated ecosystems.

Salt stress is one of the significant abiotic stress factors that exert harmful effects on plant growth and yield. In this study, five cultivars of mung bean (Vigna radiata L.) were treated with different concentrations of NaCl and also inoculated with a salt-tolerant bacterial strain to assess their growth and yield. The bacterial strain was isolated from the saline soil of Sahiwal District, Punjab, Pakistan and identified as Bacillus pseudomycoides. Plant growth was monitored at 15-days interval and finally harvested after 120 days at seed set. Both sodium and potassium uptake in above and below-ground parts were assessed using a flame photometer. Fresh and dry mass, number of pods, seeds per plant, weight of seeds per plant and weight of 100 seeds reduced significantly as the concentration of NaCl increased from 3 to 15 dSm−1. There was a significant reduction in the growth and yield of plants exposed to NaCl stress without bacterial inoculum compared to the plants with bacterial inoculum. The latter plants showed a significant increase in the studied parameters. It was found that the cultivar Inqelab mung showed the least reduction in growth and yield traits among the studied cultivars, while Ramzan mung showed the maximum reduction. Among all the cultivars, maximum Na+ uptake occurred in roots, while the least uptake was observed in seeds. The study concludes that NaCl stress significantly reduces the growth and yield of mung bean cultivars, but Bacillus pseudomycoides inoculum alleviates salt stress. These findings will be helpful to cultivate the selected cultivars in soils with varying concentrations of NaCl.

Abstract Antimicrobial resistance due to the irrational and non-sustainable use of antimicrobials poses a serious threat to animal health, human health and urban food security. This study aimed to access the knowledge and perceptions of dairy farmers regarding antimicrobial use and misuse in Lahore, Pakistan. This is the first study regarding antibiotic misuse in Lahore, Pakistan. A random sample of 270 dairy farmers from urban and suburban areas of Lahore participated in an interview-based survey conducted in 2019–20. The results were analyzed using SPSS version 16. About 22% of farmers do not consult a veterinarian, 32.9% do not follow correct dosage instructions and 39% discontinue treatment once the disease signs subside. Moreover, 40% of farmers were unaware of the dangers of misusing antibiotics and admitted to saving leftover antibiotics for future use. Alarmingly, over 20.7% of respondents share antibiotics with friends/farmers and 43% sought advice from non-veterinarian sources. Furthermore, 90% of farmers perceived self-medication as more economical than consulting a veterinarian. Dairy farmers have a wrong perception of antibiotic efficacy, use, expertise of veterinarians and cost of antibiotics. The absence of a food policy and lack of antibiotic use guidance is a serious gap in Pakistan. Antibiotic dispensing laws need to be developed and strictly implemented. Awareness campaigns need to be launched so that farmers get knowledge regarding the uses, overuse and misuse of antibiotics. A holistic approach is essential to address the potential food security crisis caused by non-sustainable farming practices. Policymakers must take action to bridge the gap in the Pakistani food supply chain and promote the sustainable use of antimicrobials/antibiotics.

Abstract The dynamic interplay between urbanization and its impacts on climate is a subject of recent concern, particularly in rapidly urbanizing cities of Pakistan. This research investigated the spatio-temporal effects of urban growth in terms of Land Use Land Cover changes on the thermal environment (Land Surface Temperature) of the Sialkot city, Pakistan using satellite data spanning four distinct time periods (1989, 2000, 2009 and 2020) and predicted changes for year 2030 by employing Cellular Automata Markov Chain Model. Satellite imagery (Landsat 5, 7 and 8) was processed, and maximum likelihood supervised classification was done to generate LULC maps for each of the aforementioned years. In addition to LULC classification, thermal bands of satellite data (for summer and winter) were processed to compute Land Surface Temperature (LST) of the city. The prediction of LULC changes and LST was done for year 2030 using Cellular Automata Markov Chain Model. The accuracy of classified and prediction maps was checked using Kappa Index. The LULC analysis revealed 4.14% increase in the built-up area and 3.43% decrease in vegetation cover of the city during 1989 to 2020. Both land covers are expected to change in the future (year 2030) by + 1.31% (built-up) and − 1.1% (vegetation). Furthermore, a declining trend in the barren land and water bodies was also observed over time. These LULC changes were found affecting the LST of study area. The transformation of vegetation cover into built-up area resulted in an increase in LST over time. A notable rise of 4.5 °C (summer) and 5.7 °C (winter) in the mean LST of Sialkot was observed during 1989 to 2020 and further increases are anticipated in year 2030. This study calls for attention of the policy makers to reduce human impact on the local climate of the city. The study will also help city developers in analyzing the urban population growth trend, finding suitable location to built new infrastructure by governmental authorities and how the rising temperature can affect energy demand and agriculture production of the city in future.

Abstract Kitchen waste is increasing globally, similarly in Pakistan bulk of municipal solid waste comprises of kitchen waste specifically, tea waste. Composting of kitchen waste is one of the promising ways to convert waste into useful product, resulting into zero waste. This study is aimed to convert waste (kitchen waste) in to a resource (compost) using bulking agents (tea waste and biochar) for reducing maturity time. Secondly, compost application on Solanum lycopersicum (tomato) was also tested. Four compost treatments were designed under aerobic composting conditions for 30 days. Tea waste and biochar have accelerated the maturity rate and produced a nutrient rich compost. Final compost had Electrical Conductivity of 2mS/cm, Carbon Nitrogen ration of 15, 54% of organic matter, 15% of moisture content, 48% of cellulose content, and 28% of Lignin content. With the use of Co-compost the Solanum lycopersicum showed 133% germination index, 100% germination, 235% Munoo-Liisa Vitality Index and 1238% seed vigor index. Co-compost also improved the soil total nitrogen by 1.4%, total phosphorous by 2%, total potassium by 2.1% and bulk density by 2.6 gcm−3. This study successfully used tea waste and biochar as bulking agents to reduce maturation time to 30 days. Tea waste and biochar enhanced the organic matter degradation, lignocellulose degradation, water holding capacity, porosity, seed’s vigor, germination index. This research can be helpful in developing home composting and home gardening to combat solid waste management and food security issue in developing countries.

This study investigated the leaf litter decomposition dynamics of Hayat-ul-Mir subtropical scrub forest in Pakistan. Litter bags were incubated for 360 days in the forest to elucidate the effect of elevation and incubation time and for 90 days in microcosms to investigate the effect of projected warming (+2.3°C and +4.5°C) and soil moisture (M15% and M20%) on litter decomposition. Site elevation significantly affected k and T50% while time affected mass loss, residual weight, remaining C and N at p

The immune system is most likely developed to reduce the harmful impact of infections on the host homeostasis. This defense approach is based on the coordinated activity of innate and adaptive immune system components, which detect and target infections for containment, killing, or expulsion by the body's defense mechanisms. These immunological processes are responsible for decreasing the pathogen burden of an infected host to maintain homeostasis that is considered to be infection resistance. Immune-driven resistance to infection is connected with a second, and probably more important, defensive mechanism: it helps to minimize the amount of dysfunction imposed on host parenchymal tissues during infection without having a direct adverse effect on pathogens. Disease tolerance is a defensive approach that relies on tissue damage control systems to prevent infections from causing harm to the host. It also uncouples immune-driven resistance mechanisms from immunopathology and disease, allowing the body to fight infection more effectively. This review discussed the cellular and molecular processes that build disease tolerance to infection and the implications of innate immunity on those systems. In addition, we discuss how symbiotic relationships with microbes and their control by particular components of innate and adaptive immunity alter disease tolerance to infection.

Despite an increased number of studies on carbon stock estimates from tropical and temperate forests, little information is available for subtropical forests, although they constitute a significant proportion of global forest cover. In this present study, we aimed to assess the above- and below ground biomass allocation strategies and carbon stocks of Acacia modesta and Olea ferruginea, which co-dominate a subtropical dry forest in Pakistan covering approximately 1.6 Mha. This scrub forest has been subjected to mining, overgrazing, and other anthropogenic activities resulting in the loss of trees and habitat degradation. For the assessment of biomass and carbon stock, 11 trees of each species representing the diameter range of 10–52 cm at 1.3 m height were harvested. The harvested A. modesta and O. ferruginea trees showed 80% and 82% of biomass in the above-ground parts and 20% and 18% to below ground parts respectively. We developed power models from the destructive harvest data for both the above- and below-ground biomass components to assess the tree-level carbon. Furthermore, we also estimated the carbon content in the leaves, roots, and shoots of both species by using a carbon analyzer, which ranged from 43.42% to 46.50%. Using the developed models, we found that the total carbon stock was 49.82 t ha−1 in the studied forest with a basal area of 27 m2 ha−1, consisting of 568 trees ha−1. This study provides useful information about the tissue carbon content, on-site models for estimating the above- and below ground biomass and carbon stock in two dominant species from a subtropical dry forest, which can be applied to estimate the carbon stock of forests in arid climate.

Limestone quarrying is an active mining practices generating bulk of solid remains and altering the habitat by the removal of plants; however, the utilization of such waste for the growth of plants has not been investigated much. The present study aimed to evaluate the effects of limestone quarry waste on the growth of two native plants by analyzing its physicochemical properties and utility for plantation purposes, while determining whether mitigation measures would be required for the habitat restoration of quarry site. Two species, Acacia modesta and Adhatoda vasica were selected from the quarry site habitat. These plants were grown in different proportions of quarry waste, and garden soil was used as a control. Growth was assessed by recording plant height, number of branches per plant, root and shoot length, and total biomass. We also analyzed the N, P, K, Na, Ca, and Mg contents of the root and shoot tissues of both species. We found a significant increase in plant height (1.24- and 1.19-fold greater than controls for A. modesta and A. vasica, respectively). Differences in the number branches, root, shoot length, and biomass were also found. A significant and positive relationship was found between the mineral content in roots and the total plant biomass across both species. We conclude that (1) the mining solid waste contained the necessary minerals for the studied plant species and no amelioration would be required for restoration of such sites with the selected indigenous plants; and (2) the quarry waste promoted the growth of the two selected species. The results of the present study can be used to plan habitat restoration in limestone mining areas that have lost plant cover.

Ergot alkaloids are novel pharmaceutical and therapeutic agents synthesized in this study using fungal species Penicillium citrinum. To get the maximum yield of ergot alkaloids a statistical process of response surface methodology was employed using surface culture fermentation technique. Initially, the strain of Penicillium was improved using physical (ultraviolet (UV) and chemical (ethyl methane sulfonate (EMS) treatments to get the maximum yield of ergot alkaloids through surface culture fermentation technique. After improving the strain, survival rate of colonies of Penicillium citrinum treated with UV and EMS was observed. Only 2.04% living colonies were observed after 150 min of exposure of Penicillium citrinum in UV light and 3.2% living colonies were observed after 20 min of the exposure in EMS. The mutated strains of Penicillium citrinum were screened for their production of ergot alkaloids and after fermentation experiments, maximum yield was obtained from PCUV-4 and PCEMS-1 strains. After strain improvement, Plackett–Burman design (PBD) and Box–Behnken design (BBD) of RSM were employed and 10-fold yield enhancement (35.60 mg/100 mL) of ergot alkaloids was achieved. This enhancement in yield of ergot alkaloids proved the positive impacts of RSM and UV on the yield of ergot alkaloids. The study provides a cost effective, economical and sustainable process to produce medically important ergot alkaloids which can be used in various pharmaceutical formulations to treat human diseases.

A bacterial consortium BMP1/SDSC/01 consisting of six isolates was isolated from textile effected soil, sludge, and textile effluent from Hudiara drain near Nishat Mills Limited, Ferozepur Road, Lahore, Pakistan. It was selected because of being capable of degrading and detoxifying red, green, black, and yellow textile dyes. The pH and supplements were optimized to enhance the decolorization ability of the selected consortium. The results indicated that decolorizing ability of consortium for the red, green, black, and yellow dyes was higher as compared to individual strains. The consortium was able to decolorize 84%, 84%, 85%, 85%, and 82% of 200 ppm of red, green, black, yellow, and mixed dyes within 24 h while individual strain required 72 h. On supplementing urea, the consortium decolorized 87, 86, 89, 86, and 83%, respectively, while on supplementing sodium chloride the consortium decolorized 93, 94, 93, 94, and 89% of red, green, black, yellow, and mixed dyes, respectively, which was maximum while in the presence of ascorbic acid and ammonium chloride it showed intermediate results. The effect of untreated and treated dyes was investigated on Zea mays L. (maize) and Sorghum vulgare Pers. (sorghum). This study will help to promote an efficient biotreatment of textile effluents.