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2. Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep

Author

Amigues Yves, Eychenne Francis, Belloc Jean-Pierre, Lajous Daniel, Bibé Bernard, Mulsant Philippe, Lecerf Frédéric, SanCristobal Magali, Bodin Loys, and Elsen Jean-Michel

Subjects
sheep, major gene, ovulation, Animal culture, SF1-1100, Genetics, QH426-470
Abstract

Abstract Inheritance of the ovulation rate (OR) in the Lacaune meat breed was studied through records from a small nucleus of 36 hyper-prolific ewes screened on farms on the basis of their natural litter size, and from progeny data of three selected Lacaune sires. These sires were chosen at the AI centre according to their breeding values estimated for the mean and the variability of their daughters' litter size. Non-carrier Lacaune dairy ewes were inseminated to produce 121 F1 daughters and 27 F1 sons. Twelve sons (four from each sire) were used in turn to inseminate non-carrier Lacaune dairy ewes providing 260 BC progeny ewes. F1 and BC progeny were brought from private farms and gathered after weaning on an experimental farm where ovulation rates were recorded in the first and second breeding seasons. With an average of 6.5 records each, the mean OR of hyper-prolific ewes was very high (5.34), and 38.4% of records showed a rate of 6 or more. F1 data showed high repeatability of OR (r = 0.54) within ewe, with significant variability among ewes. High OR (≥ 4) were observed in each family. A segregation analysis provided a significant likelihood ratio and classified the three founders as heterozygous. BC ewes also displayed high repeatability of OR (r = 0.47) and the mean OR varied considerably between families (from 1.24 to 1.78). Seven of the 12 BC families presented high-ovulating ewes (at least one record ≥ 4) and segregation analysis yielded a highly significant likelihood ratio as compared to an empirical test distribution. The high variability of the mean ovulation rate shown by a small group of daughters of BC ewes inseminated by putative carrier F1 rams, and the very high ovulation rate observed for some of these ewe lambs, confirmed the segregation of a major gene with two co-dominant alleles borne by an autosome. The difference between homozygous non-carriers and heterozygous ewes was about one ovulation on the observed scale and 2.2 standard deviations on the underlying scale.

Published
2002
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8. Using markers in gene introgression breeding programs

Author

Hospital, Frederic, Chevalet, Claude, and Mulsant, Philippe

Subjects
Genetic markers -- Research, Breeding -- Genetic aspects, Biological sciences
Abstract

A study was conducted to examine the application of genetic markers in gene introgression breeding programs. Time and intensity of selection, population size, number and position of selected markers are presented as factors which have an impact on chromosomes even with absent introgressed genes. Results show that using more than three markers is inefficient, distant markers provide better control of the introgressed gene surrounding segment and using the segment as an initial option reduces selection intensity.

Published
1992

9. Regulation of ovulation rate in mammals: contribution of sheep genetic models

Author

Persani Luca, Di Pasquale Elisa, Bodin Loys, Mulsant Philippe, Pierre Alice, Fabre Stéphane, Monget Philippe, and Monniaux Danielle

Subjects
Gynecology and obstetrics, RG1-991, Reproduction, QH471-489
Abstract

Abstract Ovarian folliculogenesis in mammals from the constitution of primordial follicles up to ovulation is a reasonably well understood mechanism. Nevertheless, underlying mechanisms that determine the number of ovulating follicles were enigmatic until the identification of the fecundity genes affecting ovulation rate in sheep, bone morphogenetic protein-15 (BMP-15), growth and differentiation factor-9 (GDF-9) and BMP receptor-1B (BMPR-1B). In this review, we focus on the use of these sheep genetic models for understanding the role of the BMP system as an intra-ovarian regulator of follicular growth and maturation, and finally, ovulation rate.

Published
2006
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10. EIF2S2 Retroposition into IRF2BP2 Underlies Fleece Variation in Sheep through the Formation of a Long Endogenous Double-Stranded RNA

Author

Demars, Julie, Cano, Margarita, Drouilhet, Laurence, Fabre, Stéphane, Servin, Bertrand, Mulsant, Philippe, Tosser-Klopp, Gwenola, Allain, Daniel, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and Instituto Nacional de Tecnología Agropecuaria (INTA)

Subjects
Animal biology, sheep, phenotypic, selection, fiber, production, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biologie animale
Abstract

International audience; Fleece variation, in composition and structure, observed in mammals is a consequence of a strong selective pressure for fiber production after domestication. Wild species with double coated fleece produced a variety of breeds with single coated fleece. In sheep, these two categories discriminate primitive sheep from woolly modern sheep (Ovis aries). Nowadays, fleece variation is segregating in the Romane breed with lambs owning a coat either long and hairy (characteristic of ancestral sheep) or short and woolly (typical to domestic sheep). Here we report that the “woolly” allele results from the insertion of an antisense EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta) retrogene (called asEIF2S2) into the 3’ UTR of the IRF2BP2 (Interferon Regulatory Factor 2 Binding Protein 2) gene leading to an abnormal IRF2BP2 transcript. We provide evidence that this chimeric IRF2BP2/asEIF2S2 messenger, by targeting the genuine sense EIF2S2 RNA and creating a long endogenous double-stranded RNA, alters the expression of EIF2S2 mRNA. This is the first example, to our knowledge of a phenotype arising from the modulation of messengers accumulation via a RNA-RNA hybrid itself generated through a retroposition gene mechanism. Altogether our results bring new insights on (i) the mammalian hair morphogenesis with the identification of EIF2S2 and IRF2BP2 as novel key regulators and (ii) the sheep population history thanks to the characterization of the molecular origin of an evolutionary phenotypic variation. Furthermore, these findings will contribute to a sustainable sheep production system since lambs carrying a hairy fleece are more adapted and robust to environmental harsh conditions.

Published
2017

11. Genome-Wide Identification of the Mutation Underlying Fleece Variation and Discriminating Ancestral Hairy Species from Modern Woolly Sheep

Author

Demars, Julie, primary, Cano, Margarita, additional, Drouilhet, Laurence, additional, Plisson-Petit, Florence, additional, Bardou, Philippe, additional, Fabre, Stéphane, additional, Servin, Bertrand, additional, Sarry, Julien, additional, Woloszyn, Florent, additional, Mulsant, Philippe, additional, Foulquier, Didier, additional, Carrière, Fabien, additional, Aletru, Mathias, additional, Rodde, Nathalie, additional, Cauet, Stéphane, additional, Bouchez, Olivier, additional, Pirson, Maarten, additional, Tosser-Klopp, Gwenola, additional, and Allain, Daniel, additional

Published
2017
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12. Fine mapping of birthcoat type in the Romanov breed sheep

Author

Cano, Margarita, Allain, Daniel, Foulquié, Didier, Moreno, Carole, Mulsant, Philippe, François, Dominique, Demars, Julie, Tosser-Klopp, Gwenola, Instituto Nacional de Tecnología Agropecuaria (INTA), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Domaine expérimental de La Fage (LA FAGE), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
marker, genomic, romane breed sheep, qtl, [SDV]Life Sciences [q-bio], birthcoat, snp, genetic, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

16. Two new mutations affecting ovulation rate in sheep

Author

Demars, Julie, Fabre, Stéphane, Rossetti, Raffaella, Persani, Luca, Tosser-Klopp, Gwenola, Mulsant, Philippe, Nowak, Zuzanna, Drobick, W., Martyniuk, E., Bodin, Loys, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratorio di Ricerche Endocrino-Metaboliche, Istituto Auxologico Italiano, Università degli Studi di Milano [Milano] (UNIMI), Warsaw University of Life Sciences (SGGW), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), ANR Monopoly, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
reproduction, sheep, [SDV]Life Sciences [q-bio], snp, [INFO]Computer Science [cs], genetic, mutation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012
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17. Novel mutations controlling ovulation rate in sheep

Author

Bodin, Loys, Demars, Julie, Drobik, W., Fabre, Stéphane, HANRAHAN, J.P., Keane, O., Martyniuk, E., Mulsant, Philippe, Nowak, Z., Persani, L., Rossetti, R., Sarry, Julien, Tosser-Klopp, Gwenola, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Warsaw University of Life Sciences (SGGW), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Teagasc Agriculture and Food Development Authority (Teagasc), Warsaw Agricultural University, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Università degli Studi di Milano [Milano] (UNIMI), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
sheep, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ovulation, mutation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

18. Goat genome assembly, availability of an international 50K SNP chip and RH panel: an update of the international goat genome consortium projects

Author

Tosser Klopp, Gwenola, Cabau, Cédric, Eggen, Andre, Faraut, Thomas, Heuven, Henri, Jamli, Saadiah, Klopp, Christophe, Taylor Lawley, Cindy, McEwan, John, Martin, Patrice, Moreno, Carole, Mulsant, Philippe, Nabihoudine, Ibouniyamine, Pailhoux, Eric, Palhière, Isabelle, Rupp, Rachel, Sarry, Julien, Sayre, Brian, Tircazes, Aurelie, Wang, Jun, Wang, Wen, Yu, Tun-Ping, Zhang, Wenguang, Génétique Physiologie et Systèmes d'Elevage, Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Faculty of Veterinary Medicine, Utrecht University [Utrecht], Unité de Biométrie et Intelligence Artificielle (UBIA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Biologie du Développement et Reproduction (BDR), UNCEIA, Capgenes and Apis-gene, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), and Plant & Animal Genome (PAG). USA.

Subjects
sheep, cattle, goat, snp, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, genome
Abstract

Goat breeds, including dairy, meat and fiber breeds, are adapted to a wide range of breeding conditions, and have a large economic impact. Currently, however, they benefit from fewer genomic research tools compared to cattle or sheep. At present, the International Goat Genome Consortium (www.goat.genome.org) federates 3 projects, aiming at the improvement of knowledge of goat genome: - de novo goat genome sequence - RH panel and whole genome high density RH maps - 50K SNP chip. An update will be made on these three topics. Details will be given on the international goat SNP chip, developed using whole genome de novo sequencing and reduced representation libraries of the genome of Alpine, Saanen, Creole, Boer, Kacang and Savanna breeds. More than 500,000 SNP were chosen by filtering on flanking sequence data, minimum allele frequency, technological qualities and categorized depending on the number of breeds in which the 2 alleles were observed. 60,000 high quality SNPs were finally spaced on the goat genome, using goat contigs. 59,000 out of the 60,000 SNPs were aligned on the bovine genome. 100 % of the SNPs were observed as heterozygous in milk breeds, 97% in meat breeds. This chip, based on Illumina Infinium technology, has been used to generate cluster files on 288 animals, using both the breeds used for SNP discovery and Skopelos, Angora and Jinlan breeds.

Published
2012

19. Towards a high density SNP International Goat Chip

Author

Tosser-Klopp, Gwenola, Jiken, Abdullah Johari, Bardou, Philippe, Cabau, Cédric, Dehais, Patrice, Faraut, Thomas, Heuven, Henri, Klopp, Christophe, Martin, Patrice, Moreno-Romieux, Carole, Mulsant, Philippe, Pailhoux, Eric, Palhiere, Isabelle, Rupp, Rachel, Sarry, Julien, Wang, J., Wang, W., Yu, Tun-Ping, Zhang, Wenguang, Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Strategic Livestock Research Centre, Malaysian Agricultural Research Development Institute, Utrecht Universtiy, Faculty of Veterinary Medicine, Unité de Biométrie et Intelligence Artificielle (UBIA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Station d'Amélioration Génétique des Animaux (SAGA), Biologie du Développement et Reproduction (BDR), BGI-Shenzhen, Bejing Genome Institute, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences [Beijing] (CAS)-Chinese Academy of Sciences [Beijing] (CAS), DNA Landmarks, caprinisp, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, AgroParisTech-Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), and Beijing Genomics Institute [Shenzhen] (BGI)

Subjects
[SDV]Life Sciences [q-bio], goat, snp
Abstract

According to FAO, in 2009, the world population of goats was estimated to be around 868 million animals and was steadily growing either in developed or developing countries. Goat breeds include dairy, meat and fiber breeds, are adapted to a wide range of breeding conditions and have thus a great economic importance. However, they benefit from fewer genomic research tools, as compared to cattle or sheep. Recently, several research projects were conducted in different laboratories to improve goat genome knowledge and develop new research tools. Goat genome was sequenced de novo (see W. Zhang presentation, this meeting), a RH panel was developed (see B. Sayre presentation, this meeting). In France, a collaborative SNP discovery research programme, CAPRISNIP, was launched by INRA and breeding organisations CAPGENES and APIS-GENE in 2009. At least two other SNP discovery projects were launched in other countries, using either reduced representations of the genome (University of Utrecht, Netherlands) or whole genome de novo sequencing (Malaysian Agricultural Research and Development Institute). RNA-sequencing projects conducted by different research groups at INRA may also be a valuable source for SNP discovery and annotation. We propose to federate these several SNP discovery and RNA-seq research programmes within the frame of the International Goat Genome Consortium in order to develop an international versatile high density SNP chip. Collection of the data is underway. SNP detection will be done in early 2011. The work plan includes i) alignment of the different sequences on de novo assembled goat contigs ii) ordering of contigs on ovine or bovine genome reference sequence iii)SNP discovery and annotation iv) small set SNP test generation v) determination of the SNP panel vi) raw data access to all partners. A first international chip order is planned for autumn 2011.

Published
2011

20. Numéro spécial 'Amélioration génétique'. Avant-propos

Author

Mulsant, Philippe, Bodin, Loys, Coudurier, Bernard, Deretz, Severine, Le Roy, Pascale, Quillet, Edwige, Perez, Jean-Marc, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Unité de Recherches Avicoles (URA), Génétique Expérimentale en Productions Animales (GEPA), Génétique Animale (GARen), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Ecole Nationale Supérieure Agronomique de Rennes, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Département Physiologie Animale et Systèmes d'Elevage (PHASE), Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Recherches Avicoles (SRA), IFR140-Ecole Nationale Supérieure Agronomique de Rennes-AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), UAR 0558 Département Physiologie Animale et Systèmes d'Elevage, Institut National de la Recherche Agronomique (INRA)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Département Physiologie Animale et Systèmes d'Elevage (DEPT PHASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST-Ecole Nationale Supérieure Agronomique de Rennes-IFR140, and AgroParisTech-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

absent

Published
2011

21. QTL detection and fine mapping of birthcoat type in sheep

Author

Allain, Daniel, Cano, M., Foulquie, Didier, Autran, Paul, Tosser-Klopp, Gwenola, Moreno, Carole, Mulsant, Philippe, François, Dominique, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Domaine expérimental de La Fage (LA FAGE), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
SHEEP, SNP50, QTL, CHOMOSOME, [SDV]Life Sciences [q-bio], GENETIC, BIRTHCOAT, PHENOTYPE, FINE MAPPING, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

23. Endocrine characterization of the reproductive axis in highly prolific Lacaune sheep homozygous for the FecL(L) mutation

Author

Drouilhet, Laurence, Taragnat, Catherine, Fontaine, Joël, Duittoz, Anne, Mulsant, Philippe, Bodin, Loys, Fabre, Stéphane, Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Supported by grants from the French 'Conseil regional Midi-Pyrénées,' 'Agence Nationale de la Recherche' GENOVUL project (ANR-05-GANI-001-01), and European Union contract SABRE (FOOD-CT-2006-01625). L.D. was supported in part by a grant from the Fonds d'Aide à la Recherche Organon (FARO)., Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
endocrine system, steroid hormone, luteinizing hormone, follicle-stimulating hormone receptor, ovary, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, genetic, granulosa cell
Abstract

Chantier qualité GA; A prolific allele named FecLL is known to segregate in the meat breed of the French Lacaune sheep on the basis of ovulation rate record. To gain more knowledge about the physiological effects of FecLL, ewes homozygous for FecLL (L/L) were compared to wild-type ewes (+/+) for ovarian phenotype and reproductive endocrine profiles. At the ovarian level, the increased ovulation rate in L/L ewes was associated with an increased number of antral follicles of greater than 3 mm and with preovulatory follicles being, on average, 1 mm smaller. Intrafollicular estradiol and testosterone concentrations were not significantly different between the two genotypes. In contrast, L/L large follicles (≥6 mm) had lower intrafollicular progesterone concentration. At the molecular level, expressions of ovarian markers, such as CYP19A1, CYP11A1, CYP17A1, LHR, and INHA, were not significantly different between the two genotypes. In contrast, FSHR and STAR mRNA levels increased in granulosa cells from L/L ewes. Plasma concentrations of estradiol, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and progesterone measured across a synchronized estrous cycle revealed a significant increase in estradiol levels during the follicular phase, a precocious LH surge, and an increase in progesterone level during the luteal phase of L/L ewes compared to +/+ ewes. Circulating concentrations of FSH were not different between the two genotypes. The precocious LH surge was associated with an increase in frequency of LH pulsatility during the follicular phase. At the pituitary level, mRNA levels for LHB, FSHB, GNRHR, and ESR1 were not significantly different between the two genotypes. In contrast, ESR2 mRNA expression was decreased in L/L ewes compared to +/+ ewes. Based on ovarian phenotype and endocrine profiles, these findings suggest that the mutation in the FecL gene affects ovarian function in a different way compared to other known prolific mutations affecting the bone morphogenetic protein signaling system in the ovine species.

Published
2010
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24. SNP detection in goats : A first step towards goat genomics

Author

Palhière, Isabelle, Moreno, Carole, Klopp, Christophe, Clément, Virginie, Lecomte, C., Martin, P., Rupp, Rachel, Mulsant, Philippe, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Unité de Biométrie et Intelligence Artificielle (UBIA), Institut de l'élevage (IDELE), France Contrôle Laitier, Capgènes, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
CAPRINS, [INFO]Computer Science [cs], [MATH]Mathematics [math], ComputingMilieux_MISCELLANEOUS, GENOMIQUE
Abstract

National audience

Published
2009

25. Effet d'un nouveau gène de prolificité sur l'endocrinologie de l'axe reproductif des brebis Lacaune

Author

Drouilhet, Laurence, Taragnat, Catherine, Duittoz, Anne, Mulsant, Philippe, Bodin, Loys, Fabre, Stéphane, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
GENETIQUE ANIMALE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2009

26. Unravelling the mechanisms of mammalian ovarian follicular development and atresia: a cattle vs pig transcriptome and proteome study

Author

Gérard, Nadine, Tosser-Klopp, Gwenola, Benne, Francis, Bonnet, Agnès, Delpuech, Thierry, Douet, Cécile, Drouilhet, Laurence, Fabre, Stéphane, Izart, A., Monget, Philippe, Monniaux, Danielle, Mulsant, Philippe, Robert Granié, Christèle, San Cristobal, Magali, Vignoles, Florence, Bodin, Loys, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], GENETIQUE, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2009

27. Exploitation de la variabilité génétique intra et inter-espèces pour l'étude du déterminisme génétique du taux d'ovulation

Author

Tosser-Klopp, Gwenola, Benne, Francis, Bodin, Loys, Bonnet, Agnès, Bontoux, M., Delpuech, Thierry, Drouilhet, Laurence, Fabre, Stéphane, Izart, A., Monget, Philippe, Monniaux, Danielle, Mulsant, Philippe, Robert-Granié, Christèle, San Cristobal, Magali, Vignoles, Florence, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
ovulation rate, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]
Published
2008

28. Caractérisation de populations à partir de l'analyse de régions chromosomiques potentiellement sélectionnées en comparaison à la diversité neutre : étude conjointe de lignées expérimentales de poules et des quatres sous-populations ovines de race Lacaune

Author

Palhière, Isabelle, Loywyck, Valérie, San Cristobal, Magali, Laloë, Denis, Amigues, Yves, Bed'Hom, Bertrand, Bibé, Bernard, Bijma, Piter, Bodin, Loys, Boudarene, Daïa, Chevalet, Claude, Leroux, Sophie, Moazami Goudarzi, Katayoun, Mulsant, Philippe, Pitel, Frédérique, Servin, Bertrand, Verrier, Etienne, Vignoles, Florence, Centre National de la Recherche Scientifique, ., Centre de Coopération Internationale en Recherche Agronomique pour le Développement, ., Institut Français de Recherche pour l'Exploitation de la Mer, ., Institut de Recherche pour le Développement, ., Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Génétique et Diversité Animales (GEDANIM), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station de Génétique Quantitative et Appliquée (SGQA), Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), Wageningen University and Research Centre (WUR), Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut de Recherche pour le Développement (IRD), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], OVIN, SHEEP, [SDV]Life Sciences [q-bio], DIVERSITY, MICROSATELLITE MARKERS, MICROSATELLITES, CHICKEN, SIGNATURE DE SELECTION, DIVERSITE, POULE, ComputingMilieux_MISCELLANEOUS, SIGNATURE OF SELECTION
Abstract

National audience

Published
2008

29. Mutations in two genes are associated with increased ovulation rate and litter size in progeny of Lacaune meat sheep

Author

Drouilhet, Laurence, Lecerf, Frédéric, BODIN, Loys, Mulsant, Philippe, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Génétique Animale (GARen), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Ecole Nationale Supérieure Agronomique de Rennes, IFR140-Ecole Nationale Supérieure Agronomique de Rennes-AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
PROLIFICITE, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, PROLIFICACY, MAJOR GENE, GENE MAJEUR, RACE OVINE LACAUNE, [SDV]Life Sciences [q-bio], LACAUNE SHEEP BREED, OVULATION
Abstract

A new autosomal major gene affecting ovulation has been evidenced by a statistical approach in the Lacaune population. A full genome scan localized this autosomal prolificacy gene (FecL) on sheep chromosome 11. Further investigations allowed the reduction of the localization interval between markers BM17132 and FAM117A, corresponding to one synteny block of human chromosome 17 of 1.1 megabases, containing 20 genes. This fine mapping is still going on. The expression of those 20 genes was tested by qPCR and no difference was found in homozygous mutants versus wild type, in four tissues (granulosa and theca cells, hypothalamus and pituitary gland). A particular haplotype was associated with the FecL mutation, which allowed marker associated classification of animals as carriers or non-carriers of the mutation. In addition, ewes with extreme ovulation rate have been isolated in the Lacaune population and it was hypothesized that another mutation was segregating in the population. A new mutation has been evidenced in the Bmp15 coding sequence (chromosome X). This mutation is a non conservative substitution (C53Y) that prevents the processing of the protein. Synergic action of the two mutations (in FecL and Bmp15 genes) on both ovulation rate and litter size was demonstrated.

Published
2008

30. Quelques enseignements tirés des mutations identifiées chez les animaux d'élevage

Author

Mulsant, Philippe, ProdInra, Migration, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], MUTATION
Published
2008

31. Exploitation de la variabilité génétique intra et inter-espèces pour l'étude du déterminisme génétique du taux d'ovulation

Author

Tosser-Klopp, Gwenola, Benne, Francis, BODIN, Loys, Bonnet, Agnès, Bontoux, Martine, Delpuech, Thierry, Drouilhet, Laurence, Fabre, S., Hatey, François, Monget, Philippe, Monniaux, Danielle, Mulsant, Philippe, Robert-Granié, Christèle, San Cristobal, Magali, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
SHEEP, OVULATION RATE, [SDV]Life Sciences [q-bio], CATTLE, PIGS, TRANSCRIPTOMICS, [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

32. Exploitation of genetic variability within and between species for the study of the genetic determinism of the rate of ovulation

Author

Mulsant, Philippe, Monget, Philippe, Bodin, Loys, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], GENETIQUE, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

33. Features of major genes for ovulation in the Lacaune population

Author

Bodin, Loys, Lecerf, Frédéric, Bessière, Marion, Mulsant, Philippe, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and ProdInra, Migration

Subjects
GENES, [SDV]Life Sciences [q-bio], GENOTYPES, [SDV] Life Sciences [q-bio], CHROMOSOMES MAPS, GENETIC MARKERS, CHROMOSOMES, GENETIC EFFECTS, LACAUNE, MUTATIONAL ANALYSIS, OVULATION, EWES, HAPLOTYPES, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

34. Exploitation de la variabilité génétique intra et inter-espèces pour l'étude du déterminisme génétique du taux d'ovulation

Author

Tosser-Klopp, Gwenola, Benne, Francis, Bonnet, Agnès, Bodin, Loys, Bontoux, M., Delpuech, Thierry, Fabre, Stéphane, Hatey, François, Monget, Philippe, Monniaux, Danielle, Mulsant, Philippe, Robert-Granié, Christèle, San Cristobal, Magali, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
ovulation rate, [SDV]Life Sciences [q-bio], gene expression, ovary, [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2006

35. A note on linkage between the angora and fgf5 genes in rabbits

Author

Mulsant, Philippe, ROCHAMBEAU, H., Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
MICE, [SDV]Life Sciences [q-bio], ANGORA GENE, GENE DU FACTEUR 5 DE CROISSANCE DU FIBROBLASTE, RABBITS, ComputingMilieux_MISCELLANEOUS, FIBROBLAST GROWTH FACTOR 5 GENE, GENE ANGORA
Abstract

International audience

Published
2004

36. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries)

Author

HANRAHAN, J.P., GREGAN, S.M., Mulsant, Philippe, MULLEN, M., DAVIS, G.H., Powell, R., GALLOWAY, S.M., ProdInra, Migration, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV] Life Sciences [q-bio], REPRODUCTION, SHEEP, MAJOR GENES, MUTATIONS, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

37. Design and Characterization of a 52K SNP Chip for Goats

Author

Amills i Eras, Marcel, Tosser-Klopp, Gwenola, Bardou, Philippe, Bouchez, Olivier, Cabau, Cédric, Crooijmans, Richard, Dong, Yang, Donnadieu-Tonon, Cécile, Eggen, André, Heuven, Henri C. M., Jamli, Saadiah, Johari Jiken, Abdullah, Klopp, Christophe, Lawley, Cynthia T., McEwan, John, Martin, Patrice, Moreno, Carole R., Mulsant, Philippe, Nabihoudine, Ibouniyamine, Pailhoux, Eric, Palhière, Isabelle, Rupp, Rachel, Sarry, Julien, Sayre, Brian L., Tircazes, Aurélie, Wang, Jun, Wang, Wen, Zhang, Wenguang, International Goat Genome Consortium, Amills i Eras, Marcel, Tosser-Klopp, Gwenola, Bardou, Philippe, Bouchez, Olivier, Cabau, Cédric, Crooijmans, Richard, Dong, Yang, Donnadieu-Tonon, Cécile, Eggen, André, Heuven, Henri C. M., Jamli, Saadiah, Johari Jiken, Abdullah, Klopp, Christophe, Lawley, Cynthia T., McEwan, John, Martin, Patrice, Moreno, Carole R., Mulsant, Philippe, Nabihoudine, Ibouniyamine, Pailhoux, Eric, Palhière, Isabelle, Rupp, Rachel, Sarry, Julien, Sayre, Brian L., Tircazes, Aurélie, Wang, Jun, Wang, Wen, Zhang, Wenguang, and International Goat Genome Consortium

Abstract

The success of Genome Wide Association Studies in the discovery of sequence variation linked to complex traits in humans has increased interest in high throughput SNP genotyping assays in livestock species. Primary goals are QTL detection and genomic selection. The purpose here was design of a 50-60,000 SNP chip for goats. The success of a moderate density SNP assay depends on reliable bioinformatic SNP detection procedures, the technological success rate of the SNP design, even spacing of SNPs on the genome and selection of Minor Allele Frequencies (MAF) suitable to use in diverse breeds. Through the federation of three SNP discovery projects consolidated as the International Goat Genome Consortium, we have identified approximately twelve million high quality SNP variants in the goat genome stored in a database together with their biological and technical characteristics. These SNPs were identified within and between six breeds (meat, milk and mixed): Alpine, Boer, Creole, Katjang, Saanen and Savanna, comprising a total of 97 animals. Whole genome and Reduced Representation Library sequences were aligned on >10 kb scaffolds of the de novo goat genome assembly. The 60,000 selected SNPs, evenly spaced on the goat genome, were submitted for oligo manufacturing (Illumina, Inc) and published in dbSNP along with flanking sequences and map position on goat assemblies (i.e. scaffolds and pseudo-chromosomes), sheep genome V2 and cattle UMD3.1 assembly. Ten breeds were then used to validate the SNP content and 52,295 loci could be successfully genotyped and used to generate a final cluster file. The combined strategy of using mainly whole genome Next Generation Sequencing and mapping on a contig genome assembly, complemented with Illumina design tools proved to be efficient in producing this GoatSNP50 chip. Advances in use of molecular markers are expected to accelerate goat genomic studies in coming years.

Published
2014

38. How many mutations are associated with increased ovulation rate and litter size in progeny of Lacaune meat sheep?

Author

BODIN, Loys, Lecerf, Frédéric, Pisselet, C., San Cristobal, Magali, Bibé, Bernard, Mulsant, Philippe, ProdInra, Migration, Génétique Animale (GARen), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Ecole Nationale Supérieure Agronomique de Rennes, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV] Life Sciences [q-bio], SHEEP, MAJOR GENES, MUTATIONS, [SDV]Life Sciences [q-bio], OVULATION
Published
2003

39. Déterminisme génétique du taux d'ovulation chez la brebis

Author

Mulsant, Philippe, ProdInra, Migration, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV] Life Sciences [q-bio], GENETIC VARIABILITY, SHEEP, [SDV]Life Sciences [q-bio], OVULATION RATE
Published
2003

40. The Booroola gene in Merinos d'Arles sheep: introduction of the gene (FecB), productivity of FecB heterozygous carrier ewes under farm conditions

Author

Teyssier, Jacques, Elsen, J.M., Bodin, Loys, Bouquet, P.M., Mulsant, Philippe, Thimonier, Jacques, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], SHEEP, PRODUCTIVITY, MAJOR GENES, MERINOS D'ARLES SHEEP BREED, [SDV]Life Sciences [q-bio], OVULATION, BOOROOLA
Published
2003

41. Localisation and mapping of a major gene controlling ovulation rate in Lacaune sheep

Author

Lecerf, Frédéric, Mulsant, Philippe, Elsen, Jean Michel, Bodin, Loys, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], LOCALISATION, GENETIQUE ANIMALE, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS, PROLOFICITE
Abstract

National audience

Published
2002

42. DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation

Author

DAVIS, G.H., GALLOWAY, S.A., ROSS, I.K., GREGAN, S.M., Ward, J., NIMBKAR, B.V., GHALSASI, P.M., NIMBKAR, C., GRAY, G.D., SUBANDRIY, O., INOUNU, I., TIESNAMURTI, B., Martyniuk, E., Eythorsdottir, E., Mulsant, Philippe, Lecerf, Frédéric, HANRAHAN, J.P., BRADFORD, G.E., Wilson, T., Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2002

43. Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep

Author

Bodin, Loys, San Cristobal, Magali, Lecerf, Frédéric, Mulsant, Philippe, Bibé, Bernard, Lajous, Daniel, Belloc, Jean-Pierre, Eychenne, Francis, Amigues, Yves, and Elsen, Jean-Michel

Subjects
sheep, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, ovulation, major gene
Abstract

DOI:10.1051/gse:2002017; International audience; Inheritance of the ovulation rate (OR) in the Lacaune meat breed was studied through records from a small nucleus of 36 hyper-prolific ewes screened on farms on the basis of their natural litter size, and from progeny data of three selected Lacaune sires. These sires were chosen at the AI centre according to their breeding values estimated for the mean and the variability of their daughters' litter size. Non-carrier Lacaune dairy ewes were inseminated to produce 121 F1 daughters and 27 F1 sons. Twelve sons (four from each sire) were used in turn to inseminate non-carrier Lacaune dairy ewes providing 260 BC progeny ewes. F1 and BC progeny were brought from private farms and gathered after weaning on an experimental farm where ovulation rates were recorded in the first and second breeding seasons. With an average of 6.5 records each, the mean OR of hyper-prolific ewes was very high (5.34), and 38.4% of records showed a rate of 6 or more. F1 data showed high repeatability of OR ($r=0.54$) within ewe, with significant variability among ewes. High OR ($\geq 4$) were observed in each family. A segregation analysis provided a significant likelihood ratio and classified the three founders as heterozygous. BC ewes also displayed high repeatability of OR ($r=0.47$) and the mean OR varied considerably between families (from 1.24 to 1.78). Seven of the 12 BC families presented high-ovulating ewes (at least one record $\geq 4$) and segregation analysis yielded a highly significant likelihood ratio as compared to an empirical test distribution. The high variability of the mean ovulation rate shown by a small group of daughters of BC ewes inseminated by putative carrier F1 rams, and the very high ovulation rate observed for some of these ewe lambs, confirmed the segregation of a major gene with two co-dominant alleles borne by an autosome. The difference between homozygous non-carriers and heterozygous ewes was about one ovulation on the observed scale and 2.2 standard deviations on the underlying scale.

Published
2002
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44. Ces bone morphogenetic proteins qui règlent le quota ovulatoire

Author

Pierre, Alice, Fabre, Stéphane, Mulsant, Philippe, Elsen, Jean-Michel, Pisselet, Claudine, Monniaux, Danielle, Monget, Philippe, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], RAT, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, TGF
Abstract

National audience

Published
2002

45. Growth factors and ovarian physiology in domestic animals

Author

Monget, Philippe, Fabre, Stéphane, Mulsant, Philippe, Lecerf, Frédéric, Elsen, Jean Michel, Mazerbourg, S., Pisselet, C., Monniaux, Danielle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2001

46. Données génomiques et identification de QTL

Author

Mulsant, Philippe, ProdInra, Migration, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV] Life Sciences [q-bio], QTL, [SDV]Life Sciences [q-bio], GENOMICS, ComputingMilieux_MISCELLANEOUS, GENOMIQUE
Abstract

International audience

Published
2001

47. Molecular genetics - Foreword

Author

Bibé, Bernard, Mulsant, Philippe, Sellier, Pierre, Département de génétique animale, Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Station de Génétique Quantitative et Appliquée (SGQA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2000

48. Cartographie fine d'un gène : bilan INRA et autres résultats marquants

Author

Mulsant, Philippe, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, CARTOGRAPHIE GENETIQUE, SELECTION ASSISTEE PAR MARQUEUR, cartographie fine, génie génétique, marqueur génétique, mutation, localisation génétique, Sciences agricoles, Agricultural sciences
Abstract

National audience; Plusieurs programmes de cartographie fine et d’identification de gènes majeurs sont actuellement en cours à l’INRA, et certains ont abouti (caséine, RN) ou devraient déboucher rapidement sur l’identification de la mutation causale (PIS, Polled, Booroola). Les caractéristiques des principaux programmes sont présentées, ainsi que les raisons de leur progression. Les possibilités d’amélioration à court terme sont discutées.

Published
2000

49. Motivations génétiques et zootechniques: le cas des lapins

Author

de Rochambeau, Hubert, Allain, Daniel, Mulsant, Philippe, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ZOOTECHNIE, GENETIQUE, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
1998

50. Regional mapping of the FecB (Booroola) region of sheep chromosome 6

Author

Mulsant, Philippe, Schibler, Laurent, Lecerf, Frédéric, Riquet, Florentine, Chitour, N., Eggen, Andre, CRIBIU, E.P., Lanneluc, I., Elsen, J.M., Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), Institut National de la Recherche Agronomique (INRA), Génétique Animale (GARen), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Ecole Nationale Supérieure Agronomique de Rennes, Station d'Amélioration Génétique des Animaux (SAGA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], sheep, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [SDV.GEN] Life Sciences [q-bio]/Genetics, [SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics, genetic, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1998

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