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Introduction: Globally, hepatocellular carcinoma (HCC) is the sixth most common malignancy and it has the fourth highest mortality. MicroRNAs play a significant part in biological processes in cell formation and advancement by targeting genes in many cancers including HCC. Objective: In the present study we examine the involvement of miR-4521 and FAM129A correlations in HCC occurrence and progression. Methods: Expression levels of miR-4521 and FAM129A in HCC tissues and cells were detected. Immunohistochemistry was carried out to detect expression of FAM129A, MMP9 and TIMP-1 in HCC tissues. Western blot assays were used to examine expression levels of different genes involve in signaling pathways. Transwell chamber, MTT and wound healing assays were performed to check cell migration, invasion and proliferation rates. Results: Overexpression of FAM129A positively correlated with upregulation of MMP9 and negatively correlated with TIMP-1 in HCC patient samples, which encouraged progression and metastasis of HCC. An antagonistic relation between miR-4521 and FAM129A was detected in current study, down-regulation of miR-4521 and up-regulation of FAM129A was demonstrated in HCC tissues and cell lines as compare to normal tissue samples and the normal cell line LO2. Overexpressing miR-4521 and silencing FAM129A impaired HCC cell migratory and invasive properties and suppressed cell proliferation. Mutually, miR-4521-FAM129A axial regulation inhibited in vitro proliferation of cells by promoting apoptosis through the p-FAK/p-AKT/MDM2/P53 and p-FAK/p-AKT/BCL-2/BAX/Cytochrome-C/Caspase-3/Caspase-9 pathways, respectively, and suppressed the migration and invasion capabilities of HCCLM3 and HepG2 cells via the TIMP-1/MMP9/MMP2 and p-FAK/p-AKT pathway. Conclusion: Our work found the axial regulation mechanism of miR-4521-FAM129A in HCC. Deficiency of miR-4521 and abundance of FAM129A synergistically enhanced cancer progression by increasing cell proliferation and malignant invasion and by inhibiting apoptosis. These discoveries suggest that miR-4521/FAM129A might play a vital role in hepatic cancer progression and could be a candidate for its therapy.

Abnormal expressions of microRNAs are involved in growth and progression of human cancers including hepatocellular carcinoma (HCC). An adaptor protein CRKL plays a pivotal role in HCC growth, whereas miR-124-3p downregulation is associated with clinical stage and the poor survival of patients. However, the relationship between miR-124-3p and CRKL and the molecular mechanisms through which they regulate HCC metastasis remains unclear. In the current work, we explored miR-124-3p and its correlation with CRKL expression in HCC patient tissues. We found that miR-124-3p deficiency is inversely co-related with CRKL overexpression in tumorous tissues of HCC patients, which was also consistent in HCCLM3 and Huh7 HCC cell lines. Target validation data shows that miR-124-3p directly targets CRKL. The overexpression of miR-124-3p reverses the CRKL expression at both mRNA and protein levels and inhibits the cell development, migration, and invasion. Mechanistic investigations showed that CRKL downregulation suppresses the ERK pathway and EMT process, and concomitant decrease in invasion and metastasis of HCC cells. The expressions of key molecules in the ERK pathway such as RAF, MEK, ERK1/2, and pERK1/2 and key promoters of EMT such as N-cadherin and vimentin were downregulated, whereas E-cadherin, a key suppression indicator of EMT, was upregulated. MiR-124-3p-mediated CRKL suppression led to BAX/BCL-2 increase and C-JUN downregulation, which inhibited the cell proliferation and promoted the apoptosis in HCC cells. Collectively, our data illustrates that miR-124-3p acts as an important tumor-suppressive miRNA to suppress HCC carcinogenesis through targeting CRKL. The miR-124-3p-CRKL axial regulated pathway may offer valuable indications for cancer research, diagnosis, and treatment.

Background: Sedentary lifestyle is associated with an increased risk of cardiovascular diseases, making it crucial to identify effective interventions to improve cardiovascular function in this population. High-intensity interval training (HIIT) has emerged as a time-efficient and effective exercise intervention to improve cardiovascular health. Aim: To investigate the effects of HIIT on cardiovascular function in sedentary individuals. Methods: Fifty sedentary individuals (25 males and 25 females) were recruited and randomly assigned into either an experimental (HIIT) or a control group. The experimental group underwent a 12-week HIIT program, while the control group maintained their sedentary lifestyle. Cardiovascular function was assessed by measuring resting heart rate (RHR), blood pressure (BP), and maximal oxygen consumption (VO2max) at baseline and after 12 weeks. Results: At baseline, there were no significant differences between the HIIT and control groups in RHR, BP, or VO2max. After 12 weeks, the HIIT group had a significant decrease in RHR (from 75.2 ± 8.3 bpm to 68.3 ± 7.5 bpm, p < 0.001) and BP (systolic BP from 124.7 ± 11.5 mmHg to 118.3 ± 9.9 mmHg, p = 0.001; diastolic BP from 78.6 ± 8.5 mmHg to 72.9 ± 6.8 mmHg, p < 0.001), and a significant increase in VO2max (from 31.6 ± 4.1 ml/kg/min to 36.8 ± 4.3 ml/kg/min, p < 0.001) compared to the control group. In contrast, there were no significant changes in RHR, BP, or VO2max in the control group. Conclusion: The results suggest that HIIT may be an effective intervention to improve cardiovascular function in sedentary individuals. These findings have important implications for developing interventions to prevent or treat cardiovascular diseases in sedentary populations. Future research should investigate the long-term effects of HIIT on cardiovascular function and other health outcomes in sedentary individuals. [ABSTRACT FROM AUTHOR]