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1. Low-dose intravenous and subcutaneous CIS43LS monoclonal antibody for protection against malaria (VRC 612 Part C): a phase 1, adaptive trial

Author

Young, Cheryl, Boyce, Colleen, Winkler, Jennifer, Holian, Susan, Greenberg, Nancy, George, Shirley, Kwon, Alyson, Dorsey, Brenda, Da Costa, Ana Raquel, Witt, William, Grays, Daryl, Gapasin, Arren, Bernal, Paula, Floyd, Jeffrey, Goldstein, Eric, Howe, Leslie, Lee, Myounghee, Marron, Jennifer, Brooks, Kelly, Turek, Lisa, Farley, Patricia, Frels, Shantel, Booth, Delores, Gall, Jason, Carlton, Kevin, Albright, Gabriela, Amharref, Nadia, Atallah, Kandace, Banappagari, Sashikanth, Bastani, Niutish, Blackstock, Daniel, Boonyaratanakornkit, Bobby, Carey, Elizabeth, Charlton, Adam, Chaudhuri, Rajoshi, Caringal, Alegria M., Chen, Mingzhong, Chen, Peifeng, Cheng, Wei, Gowetski, Daniel, Gulla, Krishana, Hastings, Erica, Horwitz, Joe, Ivleva, Vera, Kordella, Dan, Kueltzo, Lisa A., Lagler, Sara, Le, Matt, Lee, James, Lei, Paula, Li, Yile, Nagy, Attila, Patel, Aakash, Runsewe, Peyi, Shadrick, Will, Shetty, Shamitha, Wang, Hairong, Webber, Calvin, Vejzagic, Farah, Yang, Yoo-Jung, Lyke, Kirsten E, Berry, Andrea A, Mason, Kaitlin, Idris, Azza H, O'Callahan, Mark, Happe, Myra, Strom, Larisa, Berkowitz, Nina M, Guech, Mercy, Hu, Zonghui, Castro, Mike, Basappa, Manjula, Wang, Lu, Low, Kwang, Holman, LaSonji A, Mendoza, Floreliz, Gordon, Ingelise J, Plummer, Sarah H, Trofymenko, Olga, Strauss, Kathleen S, Joshi, Sudhaunshu, Shrestha, Biraj, Adams, Matthew, Chagas, Andrezza Campos, Murphy, Jittawadee R, Stein, Judy, Hickman, Somia, McDougal, Andrew, Lin, Bob, Narpala, Sandeep R, Vazquez, Sandra, Serebryannyy, Leonid, McDermott, Adrian, Gaudinski, Martin R, Capparelli, Edmund V, Coates, Emily E, Wu, Richard L, Ledgerwood, Julie E, Dropulic, Lesia K, and Seder, Robert A

Published
2023
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2. Low-dose intravenous and subcutaneous CIS43LS monoclonal antibody for protection against malaria (VRC 612 Part C): a phase 1, adaptive trial

Author

Lyke, Kirsten E, primary, Berry, Andrea A, additional, Mason, Kaitlin, additional, Idris, Azza H, additional, O'Callahan, Mark, additional, Happe, Myra, additional, Strom, Larisa, additional, Berkowitz, Nina M, additional, Guech, Mercy, additional, Hu, Zonghui, additional, Castro, Mike, additional, Basappa, Manjula, additional, Wang, Lu, additional, Low, Kwang, additional, Holman, LaSonji A, additional, Mendoza, Floreliz, additional, Gordon, Ingelise J, additional, Plummer, Sarah H, additional, Trofymenko, Olga, additional, Strauss, Kathleen S, additional, Joshi, Sudhaunshu, additional, Shrestha, Biraj, additional, Adams, Matthew, additional, Chagas, Andrezza Campos, additional, Murphy, Jittawadee R, additional, Stein, Judy, additional, Hickman, Somia, additional, McDougal, Andrew, additional, Lin, Bob, additional, Narpala, Sandeep R, additional, Vazquez, Sandra, additional, Serebryannyy, Leonid, additional, McDermott, Adrian, additional, Gaudinski, Martin R, additional, Capparelli, Edmund V, additional, Coates, Emily E, additional, Wu, Richard L, additional, Ledgerwood, Julie E, additional, Dropulic, Lesia K, additional, Seder, Robert A, additional, Young, Cheryl, additional, Boyce, Colleen, additional, Winkler, Jennifer, additional, Holian, Susan, additional, Greenberg, Nancy, additional, George, Shirley, additional, Kwon, Alyson, additional, Dorsey, Brenda, additional, Da Costa, Ana Raquel, additional, Witt, William, additional, Grays, Daryl, additional, Gapasin, Arren, additional, Bernal, Paula, additional, Floyd, Jeffrey, additional, Goldstein, Eric, additional, Howe, Leslie, additional, Lee, Myounghee, additional, Marron, Jennifer, additional, Brooks, Kelly, additional, Turek, Lisa, additional, Farley, Patricia, additional, Frels, Shantel, additional, Booth, Delores, additional, Gall, Jason, additional, Carlton, Kevin, additional, Albright, Gabriela, additional, Amharref, Nadia, additional, Atallah, Kandace, additional, Banappagari, Sashikanth, additional, Bastani, Niutish, additional, Blackstock, Daniel, additional, Boonyaratanakornkit, Bobby, additional, Carey, Elizabeth, additional, Charlton, Adam, additional, Chaudhuri, Rajoshi, additional, Caringal, Alegria M., additional, Chen, Mingzhong, additional, Chen, Peifeng, additional, Cheng, Wei, additional, Gowetski, Daniel, additional, Gulla, Krishana, additional, Hastings, Erica, additional, Horwitz, Joe, additional, Ivleva, Vera, additional, Kordella, Dan, additional, Kueltzo, Lisa A., additional, Lagler, Sara, additional, Le, Matt, additional, Lee, James, additional, Lei, Paula, additional, Li, Yile, additional, Nagy, Attila, additional, Patel, Aakash, additional, Runsewe, Peyi, additional, Shadrick, Will, additional, Shetty, Shamitha, additional, Wang, Hairong, additional, Webber, Calvin, additional, Vejzagic, Farah, additional, and Yang, Yoo-Jung, additional

Published
2023
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3. Low-Dose Subcutaneous or Intravenous Monoclonal Antibody to Prevent Malaria

Author

Wu, Richard L., primary, Idris, Azza H., additional, Berkowitz, Nina M., additional, Happe, Myra, additional, Gaudinski, Martin R., additional, Buettner, Christian, additional, Strom, Larisa, additional, Awan, Seemal F., additional, Holman, LaSonji A., additional, Mendoza, Floreliz, additional, Gordon, Ingelise J., additional, Hu, Zonghui, additional, Campos Chagas, Andrezza, additional, Wang, Lawrence T., additional, Da Silva Pereira, Lais, additional, Francica, Joseph R., additional, Kisalu, Neville K., additional, Flynn, Barbara J., additional, Shi, Wei, additional, Kong, Wing-Pui, additional, O’Connell, Sarah, additional, Plummer, Sarah H., additional, Beck, Allison, additional, McDermott, Adrian, additional, Narpala, Sandeep R., additional, Serebryannyy, Leonid, additional, Castro, Mike, additional, Silva, Rosa, additional, Imam, Marjaan, additional, Pittman, Iris, additional, Hickman, Somia P., additional, McDougal, Andrew J., additional, Lukoskie, Ashly E., additional, Murphy, Jittawadee R., additional, Gall, Jason G., additional, Carlton, Kevin, additional, Morgan, Patricia, additional, Seo, Ellie, additional, Stein, Judy A., additional, Vazquez, Sandra, additional, Telscher, Shinyi, additional, Capparelli, Edmund V., additional, Coates, Emily E., additional, Mascola, John R., additional, Ledgerwood, Julie E., additional, Dropulic, Lesia K., additional, and Seder, Robert A., additional

Published
2022
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4. Protection Against Malaria by Intravenous Immunization with a Nonreplicating Sporozoite Vaccine

Author

Seder, Robert A., Chang, Lee-Jah, Enama, Mary E., Zephir, Kathryn L., Sarwar, Uzma N., Gordon, Ingelise J., Holman, LaSonji A., James, Eric R., Billingsley, Peter F., Gunasekera, Anusha, Richman, Adam, Chakravarty, Sumana, Manoj, Anita, Velmurugan, Soundarapandian, Li, MingLin, Ruben, Adam J., Li, Tao, Eappen, Abraham G., Stafford, Richard E., Plummer, Sarah H., Hendel, Cynthia S., Novik, Laura, Costner, Pamela J. M., Mendoza, Floreliz H., Saunders, Jamie G., Nason, Martha C., Richardson, Jason H., Murphy, Jittawadee, Davidson, Silas A., Richie, Thomas L., Sedegah, Martha, Sutamihardja, Awalludin, Fahle, Gary A., Lyke, Kirsten E., Laurens, Matthew B., Roederer, Mario, Tewari, Kavita, Epstein, Judith E., Sim, B. Kim Lee, Ledgerwood, Julie E., Graham, Barney S., and Hoffman, Stephen L.

Published
2013
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7. Optimization of the CIS43LS Malarial Monoclonal Antibody and Protection Against Malaria

Author

Lyke, Kirsten E., primary, Berry, Andrea A., additional, Mason, Kaitlin, additional, Idris, Azza A., additional, O'Callahan, Mark, additional, Happe, Myra, additional, Strom, Larisa, additional, Berkowitz, Nina M., additional, Guech, Mercy, additional, Hu, Zonghui, additional, Castro, Mike, additional, Basappa, Manjula, additional, Wang, Lu, additional, Naqvi, Muhammed, additional, Low, Kwang, additional, Holman, LaSonji A., additional, Mendoza, Floreliz, additional, Gordon, Ingelize J., additional, Plummer, Sarah H., additional, Trofymenko, Olga, additional, Strauss, Kathleen S., additional, Joshi, Sudhaunshu, additional, Shrestha, Biraj, additional, Adams, Matthew, additional, Campos Chagas, Andrezza, additional, Murphy, Jittawadee R., additional, Stein, Judy A., additional, Hickman, Somia P., additional, McDougal, Andrew J., additional, Lin, Bob, additional, Narparla, Sandeep, additional, Vazquez, Sandra, additional, Serebryannyy, Leonid, additional, McDermott, Adrian, additional, Gaudinski, Martin R., additional, Capparelli, Edmund V., additional, Coates, Emily E., additional, Wu, Richard L., additional, Ledgerwood, Julie E., additional, Dropulic, Lesia K., additional, and Seder, Robert A., additional

Published
2022
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8. Human adenovirus 5-vectored Plasmodium falciparum NMRC-M3V-Ad-PfCA vaccine encoding CSP and AMA1 is safe, well-tolerated and immunogenic but does not protect against controlled human malaria infection

Author

Tamminga, Cindy, Sedegah, Martha, Maiolatesi, Santina, Fedders, Charlotte, Reyes, Sharina, Reyes, Anatalio, Vasquez, Carlos, Alcorta, Yolanda, Chuang, Ilin, Spring, Michele, Kavanaugh, Michael, Ganeshan, Harini, Huang, Jun, Belmonte, Maria, Abot, Esteban, Belmonte, Arnel, Banania, JoGlenna, Farooq, Fouzia, Murphy, Jittawadee, Komisar, Jack, Richie, Nancy O, Bennett, Jason, Limbach, Keith, Patterson, Noelle B, Bruder, Joseph T, Shi, Meng, Miller, Edward, Dutta, Sheetij, Diggs, Carter, Soisson, Lorraine A, Hollingdale, Michael R, Epstein, Judith E, and Richie, Thomas L

Published
2013

9. Various levels of cross-resistance to Bacillus sphaericus strains in Culex pipiens (Diptera: Culicidae) colonies resistant to B. sphaericus strain 2362

Author

Nielsen-LeRoux, Christina, Rao, D. Raghunatha, Murphy, Jittawadee Rodcharoen, Carron, Alexandre, Mani, T.R., Hamon, Sylviane, and Mulla, Mir S.

Subjects
Insect pests -- Biological control, Mosquitoes -- Control, Bacillus (Bacteria) -- Research, Biological sciences
Abstract

Bacillus sphaericus strain IAB-59 is toxic to species of Culex pipiens that are resistant to other strains of B. sphaericus. B. sphaericus has been used as a biological control agent for Culex pipiens mosquito larvae since the late 1980s.

Published
2001

10. Distribution of Triatoma dimidiatasensu lato (Reduviidae: Triatominae) and Risk Factors Associated with Household Invasion in Northern Belize, Central America

Author

Caranci, Angela T, Grieco, John P, Achee, Nicole L, Hoel, David F, Bautista, Kim, King, Russell, Stewart, V Ann, Murphy, Jittawadee, Masuoka, Penny, and Olsen, Cara H

Abstract

To date, Triatoma dimidiatasensu lato [Reduviidae: Triatominae (Latreille 1811)] remains the sole vector species associated with Chagas disease transmission reported from Belize. Human infection data are limited for Belize and the disease transmission dynamics have not been thoroughly investigated, yet the likelihood of autochthonous transmission is supported by the widespread collection of infected vectors from within local households. Here, we report updated infection rates of the vector population and infestation rates for villages in north and central Belize. Overall, 275 households were enrolled in an ongoing vector surveillance program. Of the 41 insects collected, 25 were PCR positive for T. cruzi, indicating an infection rate as high as 60%. To further characterize the epidemiological risk of human–vector contact, determinants of household invasion were modeled. Local households were surveyed and characterized with respect to over 25 key factors that may be associated with household infestation by T. dimidiatas.l. While final models were not strongly predictive with respect to the risk factors that were surveyed, likely due to the low number of collection observations, the presence of domestic/peri-domestic dogs, nearby light sources, and household structure materials could be the focus of continued risk assessments. In northern Belize, this vector survey lends support to T. dimidiatas.l. inhabiting sylvatic settings as opposed to the classical paradigm of domiciliated vector populations. This designation has strong implications for the local level of human exposure risk which can help guide vector surveillance and control resources.

Published
2022
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11. Mosquito bisection as a variable in estimates of PCR-derived malaria sporozoite rates

Author

Foley Desmond H, Harrison Genelle, Murphy Jittawadee R, Dowler Megan, Rueda Leopoldo M, and Wilkerson Richard C

Subjects
Plasmodium, Malaria, Sporozoite rate, Oocyst, PCR, Dissection, Anopheles, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Highly sensitive polymerase chain reaction (PCR) methods offer an alternative to the light microscopy examination of mosquito salivary glands for the determination of malaria sporozoite rates in wild caught female Anopheles. Removal of mosquito abdomens is assumed to eliminate false positives caused by malaria oocyst DNA in the midgut. This assumption has not been tested with current gold standard PCR assays, and for the variety of conditions that specimens could encounter in the laboratory and field. Methods Laboratory Anopheles stephensi were used that had been infected with Plasmodium falciparum 6–7 days and 14 days post infection (p.i.), when oocysts only and oocysts + sporozoites, respectively, are developed. Mosquitoes were killed and immediately frozen, air dried before being frozen, or stored under humid conditions overnight before being frozen, to simulate a range of conditions in the field. Additionally, abdomens were removed anterior to, at, or posterior to the junction of the abdomen and thorax, and both portions were processed using a standard nested PCR of the small sub-unit nuclear ribosomal genes (ssrDNA) with products visualized on agarose gels. Results Overall, 4.1 % (4/97) of head + thorax samples that were 6–7 days p.i. gave apparent false positives for sporozoites, compared to 9.3 % (9/97) that were positive for abdomens. No positives (0/52) were obtained when similar specimens were bisected anterior to the junction of the thorax and abdomen, compared to 21.2 % (11/52) that were positive for posterior portions. Multiple bands were noted for positives from the ‘Frozen’ treatment and the rate of false negatives due to DNA degradation appears higher under the ‘Humid’ treatment. Reproducibility of results for the ‘Frozen’ treatment was 90 %. Conclusions Despite the importance of specimen condition and the bisection step in determining sporozoite rates, little attention has been paid to them in the literature. Recommendations from this study are that: 1) care needs to be taken to reduce DNA degradation in the field; 2) mosquito abdomens be separated anterior to the junction of the thorax and abdomen; and 3) DNA sequencing of a subsample of positive results should be undertaken if possible.

Published
2012
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12. Molecular Detection and Identification of Rickettsia Species in Ticks (Acari: Ixodidae) Collected From Belize, Central America

Author

Polsomboon, Suppaluck, primary, Hoel, David F, additional, Murphy, Jittawadee R, additional, Linton, Yvonne-Marie, additional, Motoki, Maysa, additional, Robbins, Richard G, additional, Bautista, Kim, additional, Briceño, Ireneo, additional, Achee, Nicole L, additional, Grieco, John P, additional, Ching, Wei-Mei, additional, and Chao, Chien-Chung, additional

Published
2017
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15. Phase 1/2a Trial of Plasmodium vivax Malaria Vaccine Candidate VMP001/AS01B in Malaria-Naive Adults: Safety, Immunogenicity, and Efficacy

Author

Bennett, Jason W., primary, Yadava, Anjali, additional, Tosh, Donna, additional, Sattabongkot, Jetsumon, additional, Komisar, Jack, additional, Ware, Lisa A., additional, McCarthy, William F., additional, Cowden, Jessica J., additional, Regules, Jason, additional, Spring, Michele D., additional, Paolino, Kristopher, additional, Hartzell, Joshua D., additional, Cummings, James F., additional, Richie, Thomas L., additional, Lumsden, Joanne, additional, Kamau, Edwin, additional, Murphy, Jittawadee, additional, Lee, Cynthia, additional, Parekh, Falgunee, additional, Birkett, Ashley, additional, Cohen, Joe, additional, Ballou, W. Ripley, additional, Polhemus, Mark E., additional, Vanloubbeeck, Yannick F., additional, Vekemans, Johan, additional, and Ockenhouse, Christian F., additional

Published
2016
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16. Detection of Dengue Virus in Mosquito Extracts and Human Clinical Samples Using a Field Expedient Molecular Platform

Author

Pal, Subhamoy, primary, Richardson, Jason H., additional, Murphy, Jittawadee R., additional, Krairojananan, Panadda, additional, Kongtak, Patcharee, additional, Jaichapor, Boonsong, additional, Kankaew, Prasan, additional, Ekanayake, Sajeewane, additional, Davis, Timothy J., additional, Maserang, David L., additional, Teng, David H. F., additional, Crisp, Robert J., additional, Wu, Shuenn-Jue L., additional, Coleman, Russell E., additional, McAvin, James C., additional, and Swaby, James A., additional

Published
2015
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18. Using infective mosquitoes to challenge monkeys with Plasmodium knowlesi in malaria vaccine studies

Author

Murphy, Jittawadee R, primary, Weiss, Walter R, additional, Fryauff, David, additional, Dowler, Megan, additional, Savransky, Tatyana, additional, Stoyanov, Cristina, additional, Muratova, Olga, additional, Lambert, Lynn, additional, Orr-Gonzalez, Sachy, additional, Zeleski, Katie Lynn, additional, Hinderer, Jessica, additional, Fay, Michael P, additional, Joshi, Gyan, additional, Gwadz, Robert W, additional, Richie, Thomas L, additional, Villasante, Eileen Franke, additional, Richardson, Jason H, additional, Duffy, Patrick E, additional, and Chen, Jingyang, additional

Published
2014
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20. Human adenovirus 5-vectoredPlasmodium falciparumNMRC-M3V-Ad-PfCA vaccine encoding CSP and AMA1 is safe, well-tolerated and immunogenic but does not protect against controlled human malaria infection

Author

Tamminga, Cindy, primary, Sedegah, Martha, additional, Maiolatesi, Santina, additional, Fedders, Charlotte, additional, Reyes, Sharina, additional, Reyes, Anatalio, additional, Vasquez, Carlos, additional, Alcorta, Yolanda, additional, Chuang, Ilin, additional, Spring, Michele, additional, Kavanaugh, Michael, additional, Ganeshan, Harini, additional, Huang, Jun, additional, Belmonte, Maria, additional, Abot, Esteban, additional, Belmonte, Arnel, additional, Banania, JoGlenna, additional, Farooq, Fouzia, additional, Murphy, Jittawadee, additional, Komisar, Jack, additional, Richie, Nancy O, additional, Bennett, Jason, additional, Limbach, Keith, additional, Patterson, Noelle B, additional, Bruder, Joseph T, additional, Shi, Meng, additional, Miller, Edward, additional, Dutta, Sheetij, additional, Diggs, Carter, additional, Soisson, Lorraine A, additional, Hollingdale, Michael R, additional, Epstein, Judith E, additional, and Richie, Thomas L, additional

Published
2013
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21. First-in-human evaluation of genetically attenuated Plasmodium falciparum sporozoites administered by bite of Anopheles mosquitoes to adult volunteers

Author

Spring, Michele, primary, Murphy, Jittawadee, additional, Nielsen, Robin, additional, Dowler, Megan, additional, Bennett, Jason W., additional, Zarling, Stasya, additional, Williams, Jack, additional, de la Vega, Patricia, additional, Ware, Lisa, additional, Komisar, Jack, additional, Polhemus, Mark, additional, Richie, Thomas L., additional, Epstein, Judy, additional, Tamminga, Cindy, additional, Chuang, Ilin, additional, Richie, Nancy, additional, O’Neil, Michael, additional, Heppner, D. Gray, additional, Healer, Julie, additional, O’Neill, Matthew, additional, Smithers, Hannah, additional, Finney, Olivia C., additional, Mikolajczak, Sebastian A., additional, Wang, Ruobing, additional, Cowman, Alan, additional, Ockenhouse, Christian, additional, Krzych, Urszula, additional, and Kappe, Stefan H.I., additional

Published
2013
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22. Transgenic Parasites Stably Expressing Full-Length Plasmodium falciparum Circumsporozoite Protein as a Model for Vaccine Down-Selection in Mice Using Sterile Protection as an Endpoint

Author

Porter, Michael D., primary, Nicki, Jennifer, additional, Pool, Christopher D., additional, DeBot, Margot, additional, Illam, Ratish M., additional, Brando, Clara, additional, Bozick, Brooke, additional, De La Vega, Patricia, additional, Angra, Divya, additional, Spaccapelo, Roberta, additional, Crisanti, Andrea, additional, Murphy, Jittawadee R., additional, Bennett, Jason W., additional, Schwenk, Robert J., additional, Ockenhouse, Christian F., additional, and Dutta, Sheetij, additional

Published
2013
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23. DNA Prime/Adenovirus Boost Malaria Vaccine Encoding P. falciparum CSP and AMA1 Induces Sterile Protection Associated with Cell-Mediated Immunity

Author

Chuang, Ilin, primary, Sedegah, Martha, additional, Cicatelli, Susan, additional, Spring, Michele, additional, Polhemus, Mark, additional, Tamminga, Cindy, additional, Patterson, Noelle, additional, Guerrero, Melanie, additional, Bennett, Jason W., additional, McGrath, Shannon, additional, Ganeshan, Harini, additional, Belmonte, Maria, additional, Farooq, Fouzia, additional, Abot, Esteban, additional, Banania, Jo Glenna, additional, Huang, Jun, additional, Newcomer, Rhonda, additional, Rein, Lisa, additional, Litilit, Dianne, additional, Richie, Nancy O., additional, Wood, Chloe, additional, Murphy, Jittawadee, additional, Sauerwein, Robert, additional, Hermsen, Cornelus C., additional, McCoy, Andrea J., additional, Kamau, Edwin, additional, Cummings, James, additional, Komisar, Jack, additional, Sutamihardja, Awalludin, additional, Shi, Meng, additional, Epstein, Judith E., additional, Maiolatesi, Santina, additional, Tosh, Donna, additional, Limbach, Keith, additional, Angov, Evelina, additional, Bergmann-Leitner, Elke, additional, Bruder, Joseph T., additional, Doolan, Denise L., additional, King, C. Richter, additional, Carucci, Daniel, additional, Dutta, Sheetij, additional, Soisson, Lorraine, additional, Diggs, Carter, additional, Hollingdale, Michael R., additional, Ockenhouse, Christian F., additional, and Richie, Thomas L., additional

Published
2013
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24. Phase 1/2a Trial of Plasmodium vivax Malaria Vaccine Candidate VMP001/AS01B in Malaria-Naive Adults: Safety, Immunogenicity, and Efficacy.

Author

Bennett, Jason W., Yadava, Anjali, Tosh, Donna, Sattabongkot, Jetsumon, Komisar, Jack, Ware, Lisa A., McCarthy, William F., Cowden, Jessica J., Regules, Jason, Spring, Michele D., Paolino, Kristopher, Hartzell, Joshua D., Cummings, James F., Richie, Thomas L., Lumsden, Joanne, Kamau, Edwin, Murphy, Jittawadee, Lee, Cynthia, Parekh, Falgunee, and Birkett, Ashley

Subjects
MALARIA vaccines, PLASMODIUM vivax, MALARIA treatment, CIRCUMSPOROZOITE protein, IMMUNE response, PARASITEMIA
Abstract

Background: A vaccine to prevent infection and disease caused by Plasmodium vivax is needed both to reduce the morbidity caused by this parasite and as a key component in efforts to eradicate malaria worldwide. Vivax malaria protein 1 (VMP001), a novel chimeric protein that incorporates the amino- and carboxy- terminal regions of the circumsporozoite protein (CSP) and a truncated repeat region that contains repeat sequences from both the VK210 (type 1) and the VK247 (type 2) parasites, was developed as a vaccine candidate for global use. Methods: We conducted a first-in-human Phase 1 dose escalation vaccine study with controlled human malaria infection (CHMI) of VMP001 formulated in the GSK Adjuvant System AS01B. A total of 30 volunteers divided into 3 groups (10 per group) were given 3 intramuscular injections of 15μg, 30μg, or 60μg respectively of VMP001, all formulated in 500μL of AS01B at each immunization. All vaccinated volunteers participated in a P. vivax CHMI 14 days following the third immunization. Six non-vaccinated subjects served as infectivity controls. Results: The vaccine was shown to be well tolerated and immunogenic. All volunteers generated robust humoral and cellular immune responses to the vaccine antigen. Vaccination did not induce sterile protection; however, a small but significant delay in time to parasitemia was seen in 59% of vaccinated subjects compared to the control group. An association was identified between levels of anti-type 1 repeat antibodies and prepatent period. Significance: This trial was the first to assess the efficacy of a P. vivax CSP vaccine candidate by CHMI. The association of type 1 repeat-specific antibody responses with delay in the prepatency period suggests that augmenting the immune responses to this domain may improve strain-specific vaccine efficacy. The availability of a P. vivax CHMI model will accelerate the process of P. vivax vaccine development, allowing better selection of candidate vaccines for advancement to field trials. [ABSTRACT FROM AUTHOR]

Published
2016
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26. Clinical Evaluation Of New Viral Vectored Vaccines Targeting The Plasmodium Falciparum Blood-Stage Antigens; Msp1 And Ama1

Author

Sheehy, Susanne, primary, Duncan, Christopher, additional, Anagnostou, Nicholas, additional, Elias, Sean, additional, Halstead, Fenella, additional, Collins, Katharine, additional, Ewer, Katie, additional, Edwards, Nick, additional, Douglas, Alexander, additional, Gantlett, Katherine, additional, Lawrie, Alison, additional, Berrie, Eleanor, additional, Moyles, Sarah, additional, Long, Carole, additional, Sinden, Robert, additional, Blagborough, Andrew, additional, Murphy, Jittawadee, additional, Nicosia, Alfredo, additional, Hill, Adrian, additional, and Draper, Simon, additional

Published
2011
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27. Prolonged Protection Provided by a Single Dose of Atovaquone-Proguanil for the Chemoprophylaxis of Plasmodium falciparum Malaria in a Human Challenge Model

Author

Deye, Gregory A., primary, Miller, R. Scott, additional, Miller, Lori, additional, Salas, Carola J., additional, Tosh, Donna, additional, Macareo, Louis, additional, Smith, Bryan L., additional, Fracisco, Susan, additional, Clemens, Emily G., additional, Murphy, Jittawadee, additional, Sousa, Jason C., additional, Dumler, J. Stephen, additional, and Magill, Alan J., additional

Published
2011
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28. Adenovirus-5-Vectored P. falciparum Vaccine Expressing CSP and AMA1. Part B: Safety, Immunogenicity and Protective Efficacy of the CSP Component

Author

Tamminga, Cindy, primary, Sedegah, Martha, additional, Regis, David, additional, Chuang, Ilin, additional, Epstein, Judith E., additional, Spring, Michele, additional, Mendoza-Silveiras, Jose, additional, McGrath, Shannon, additional, Maiolatesi, Santina, additional, Reyes, Sharina, additional, Steinbeiss, Victoria, additional, Fedders, Charlotte, additional, Smith, Kathryn, additional, House, Brent, additional, Ganeshan, Harini, additional, Lejano, Jennylynn, additional, Abot, Esteban, additional, Banania, Glenna J., additional, Sayo, Renato, additional, Farooq, Fouzia, additional, Belmonte, Maria, additional, Murphy, Jittawadee, additional, Komisar, Jack, additional, Williams, Jackie, additional, Shi, Meng, additional, Brambilla, Donald, additional, Manohar, Nalini, additional, Richie, Nancy O., additional, Wood, Chloe, additional, Limbach, Keith, additional, Patterson, Noelle B., additional, Bruder, Joseph T., additional, Doolan, Denise L., additional, King, C. Richter, additional, Diggs, Carter, additional, Soisson, Lorraine, additional, Carucci, Daniel, additional, Levine, Gail, additional, Dutta, Sheetij, additional, Hollingdale, Michael R., additional, Ockenhouse, Christian F., additional, and Richie, Thomas L., additional

Published
2011
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29. Human adenovirus 5-vectored Plasmodium falciparumNMRC-M3V-Ad-PfCA vaccine encoding CSP and AMA1 is safe, well-tolerated and immunogenic but does not protect against controlled human malaria infection

Author

Tamminga, Cindy, Sedegah, Martha, Maiolatesi, Santina, Fedders, Charlotte, Reyes, Sharina, Reyes, Anatalio, Vasquez, Carlos, Alcorta, Yolanda, Chuang, Ilin, Spring, Michele, Kavanaugh, Michael, Ganeshan, Harini, Huang, Jun, Belmonte, Maria, Abot, Esteban, Belmonte, Arnel, Banania, JoGlenna, Farooq, Fouzia, Murphy, Jittawadee, Komisar, Jack, Richie, Nancy O, Bennett, Jason, Limbach, Keith, Patterson, Noelle B, Bruder, Joseph T, Shi, Meng, Miller, Edward, Dutta, Sheetij, Diggs, Carter, Soisson, Lorraine A, Hollingdale, Michael R, Epstein, Judith E, and Richie, Thomas L

Abstract

Background: In a prior study, a DNA prime / adenovirus boost vaccine (DNA/Ad) expressing P. falciparumcircumsporozoite protein (CSP) and apical membrane antigen-1 (AMA1) (NMRC-M3V-D/Ad-PfCA Vaccine) induced 27% protection against controlled human malaria infection (CHMI). To investigate the contribution of DNA priming, we tested the efficacy of adenovirus vaccine alone (NMRC-M3V-Ad-PfCA ) in a Phase 1 clinical trial.Methodology/Principal Findings: The regimen was a single intramuscular injection with two non-replicating human serotype 5 adenovectors encoding CSP and AMA1, respectively. One x 1010particle units of each construct were combined prior to administration. The regimen was safe and well-tolerated. Four weeks later, 18 study subjects received P. falciparumCHMI administered by mosquito bite. None were fully protected although one showed delayed onset of parasitemia. Antibody responses were low, with geometric mean CSP ELISA titer of 381 (range < 50–1626) and AMA1 ELISA of 4.95 µg/mL (range 0.2–38). Summed ex vivo IFN-γ ELISpot responses to overlapping peptides were robust, with geometric mean spot forming cells/million peripheral blood mononuclear cells [sfc/m] for CSP of 273 (range 38–2550) and for AMA1 of 1303 (range 435–4594). CD4+ and CD8+ T cell IFN-γ responses to CSP were positive by flow cytometry in 25% and 56% of the research subjects, respectively, and to AMA1 in 94% and 100%, respectively.Significance: In contrast to DNA/Ad, Ad alone did not protect against CHMI despite inducing broad, cell-mediated immunity, indicating that DNA priming is required for protection by the adenovirus-vectored vaccine. ClinicalTrials.gov Identifier: NCT00392015.

Published
2013
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30. Transgenic Parasites Stably Expressing Full-Length Plasmodium falciparumCircumsporozoite Protein as a Model for Vaccine Down-Selection in Mice Using Sterile Protection as an Endpoint

Author

Porter, Michael D., Nicki, Jennifer, Pool, Christopher D., DeBot, Margot, Illam, Ratish M., Brando, Clara, Bozick, Brooke, De La Vega, Patricia, Angra, Divya, Spaccapelo, Roberta, Crisanti, Andrea, Murphy, Jittawadee R., Bennett, Jason W., Schwenk, Robert J., Ockenhouse, Christian F., and Dutta, Sheetij

Abstract

ABSTRACTCircumsporozoite protein (CSP) of Plasmodium falciparumis a protective human malaria vaccine candidate. There is an urgent need for models that can rapidly down-select novel CSP-based vaccine candidates. In the present study, the mouse-mosquito transmission cycle of a transgenic Plasmodium bergheimalaria parasite stably expressing a functional full-length P. falciparumCSP was optimized to consistently produce infective sporozoites for protection studies. A minimal sporozoite challenge dose was established, and protection was defined as the absence of blood-stage parasites 14 days after intravenous challenge. The specificity of protection was confirmed by vaccinating mice with multiple CSP constructs of differing lengths and compositions. Constructs that induced high NANP repeat-specific antibody titers in enzyme-linked immunosorbent assays were protective, and the degree of protection was dependent on the antigen dose. There was a positive correlation between antibody avidity and protection. The antibodies in the protected mice recognized the native CSP on the parasites and showed sporozoite invasion inhibitory activity. Passive transfer of anti-CSP antibodies into naive mice also induced protection. Thus, we have demonstrated the utility of a mouse efficacy model to down-select human CSP-based vaccine formulations.

Published
2013
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31. Selected Papers from the International Conference on Biopesticides 6 (ICOB6).

Author

Kabkaew L. Sukontason, Mulla, Mir S., Wongsiri, Siriwat, Trumble, John T., and Murphy, Jittawadee R.

Subjects
APPLICATION of pesticides, BIOLOGICAL pest control, FUNGICIDES
Abstract

An introduction is presented in which the editors discuss various papers within the issue on the role of Biopesticides 6 including examining of some crude plants' fungicide activity, the mode of action of botanical insecticides and the efficacy of essential oils derived from insects.

Published
2012
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32. Effects of Preexposure to DEET on the Downstream Blood-Feeding Behaviors of Aedes aegypti (Diptera: Culicidae) Mosquitoes

Author

Sugiharto, Victor A., Grieco, John P., Murphy, Jittawadee R., Olsen, Cara H., Colacicco-Mayhugh, Michelle G., Stewart, V. Ann, Achee, Nicole L., and Turell, Michael J.

Abstract

Mosquito behavior is heavily influenced by the chemical molecules in the environment. This knowledge can be used to modify insect behaviors; particularly to reduce vector–host contact as a powerful method for disease prevention. N,N-Diethyl-meta-toluamide (DEET) is the most widely used insect repellent in the market and an excellent example of a chemical that has been used to modify insect behavior for disease prevention. However, genetic insensitivity and habituation in Aedes aegypti (L.) mosquitoes after preexposure to DEET have been reported. In this study, we investigated the effect of preexposure to DEET on the downstream blood-feeding behavior of Ae. aegypti mosquitoes and the duration of the effect. We exposed mosquitoes to four different DEET concentrations: 0.10, 0.12, 0.14, and 0.16% for 10 min then allowed the mosquitoes to blood-feed on an artificial blood-feeding system either immediately or after being held for 1, 3, 6, or 24 h following DEET exposure. We found that preexposing Ae. aegypti mosquitoes to 0.14 or 0.16% DEET lowered their blood engorgement level, but did not alter their landing and probing behavior when compared to the control test populations. The reduction in complete blood-feeding was observed at all time periods tested, but was only statistically significant at 3 and 6 h after the preexposure process. Because reduction in blood meal has been associated with increased refeeding, future studies analyzing the effect of this behavior using arbovirus-infected mosquitoes are needed to address the concern of potentially increased vectorial capacity.

Published
2016
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34. Effect of temperature on laboratory reared Anopheles dirus Peyton and Harrison and Anopheles sawadwongporni Rattanarithikul and Green.

Author

Phasomkusolsil S, Lerdthusnee K, Khuntirat B, Kongtak W, Pantuwatana K, and Murphy JR

Subjects
Animals, Animals, Laboratory, Body Size, Female, Fertility, Male, Temperature, Thailand, Anopheles growth & development
Abstract

Investigations have shown that female mosquitoes with a larger body size (determined by wing length) exhibit higher feeding rates and greater fecundity relative to smaller mosquitoes. In this study, Anopheles dirus and An. sawadwongporni were reared in the laboratory at two different temperatures (23 degrees C and 30 degrees C). Effects of the rearing temperature on body size, fecundity, and larval development period were examined by measuring wing length, adult body weight at emergence, the number of eggs produced and the length of time from the first to the fourth instar. Rearing temperature had a direct effect on body size, fecundity and larval development period for both species. Mosquitoes of both species reared at 23 degrees C were larger in body size, experienced prolonged development and produced a larger clutch of eggs relative to mosquitoes reared at 30 degrees C. However, there was no temperature effect on egg hatching rate and sex ratio.

Published
2011

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