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11 results on '"Mursalimov, Sergey R."'

3. The recombinant fusion protein CFP10–ESAT6–dIFN has protective effect against tuberculosis in guinea pigs

Author

Permyakova, Natalya V., Belavin, Pavel A., Pirozhkova, Dariya S., Ufimtseva, Elena G., Rozov, Sergey M., Mursalimov, Sergey R., Sidorchuk, Yuriy V., Uvarova, Elena A., Zagorskaya, Alla A., Marenkova, Tatiana V., Bannikova, Svetlana V., Demidov, Evgeniy A., Starostin, Konstantin V., Kravchenko, Marionella A., Vakhrusheva, Diana V., Berdnikov, Roman B., Eremeeva, Natalya I., Skornyakov, Sergey N., Peltek, Sergey E., and Deineko, Elena V.

Abstract

Development of effective vaccine candidates against tuberculosis is currently the most important challenge in the prevention of this disease since the BCG vaccine fails to guarantee a lifelong protection, while any other approved vaccine with better efficiency is still absent. The protective effect of the recombinant fusion protein ESAT6–CFP10–dIFN produced in a prokaryotic expression system (Escherichia coli) has been assessed in a guinea pig model of acute tuberculosis. The tested antigen comprises the Mycobacterium tuberculosis(Mtb) proteins ESAT6 and CFP10 as well as modified human γ-interferon (dIFN) for boosting the immune response. Double intradermal immunization of animals with the tested fusion protein (2 × 0.5 μg) induces a protective effect against subsequent Mtbinfection. The immunized animals do not develop the symptoms of acute tuberculosis and their body weight gain was five times more as compared with the non-immunized-infected animals. The animal group immunized with this dose of antigen displays the minimum morphological changes in the internal organs and insignificant inflammatory lesions in the liver tissue, which complies with a decrease in the bacterial load in the spleen and average Mtbcounts in macrophages.

Published
2024
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8. The recombinant fusion protein CFP10–ESAT6–dIFN has protective effect against tuberculosis in guinea pigs

Author

Permyakova, Natalya V., primary, Belavin, Pavel A., additional, Pirozhkova, Dariya S., additional, Ufimtseva, Elena G., additional, Rozov, Sergey M., additional, Mursalimov, Sergey R., additional, Sidorchuk, Yuriy V., additional, Uvarova, Elena A., additional, Zagorskaya, Alla A., additional, Marenkova, Tatiana V., additional, Bannikova, Svetlana V., additional, Demidov, Evgeniy A., additional, Starostin, Konstantin V., additional, Kravchenko, Marionella A., additional, Vakhrusheva, Diana V., additional, Berdnikov, Roman B., additional, Eremeeva, Natalya I., additional, Skornyakov, Sergey N., additional, Peltek, Sergey E., additional, and Deineko, Elena V., additional

Published
2018
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9. The recombinant fusion protein CFP10–ESAT6–dIFN has protective effect against tuberculosis in guinea pigs

Author

Permyakova, Natalya V., primary, Belavin, Pavel A., additional, Pirozhkova, Dariya S., additional, Ufimtseva, Elena G., additional, Rozov, Sergey M., additional, Mursalimov, Sergey R., additional, Sidorchuk, Yuriy V., additional, Uvarova, Elena A., additional, Zagorskaya, Alla A., additional, Marenkova, Tatiana V., additional, Bannikova, Svetlana V., additional, Demidov, Evgeniy A., additional, Starostin, Konstantin V., additional, Kravchenko, Marionella A., additional, Vakhrusheva, Diana V., additional, Berdnikov, Roman B., additional, Eremeeva, Natalya I., additional, Skornyakov, Sergey N., additional, Peltek, Sergey E., additional, and Deineko, Elena V., additional

Published
2017
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11. Cytological Techniques to Study Cytomixis in Plant Male Meiosis.

Author

Mursalimov SR, Sidorchuk YV, and Deineko EV

Subjects
In Situ Hybridization, Fluorescence, Plant Cells, Nicotiana genetics, Cytogenetic Analysis methods, Meiosis, Plants genetics
Abstract

In this chapter we describe cytological techniques to study cytomixis, a process of nuclear migration between plant cells, in squashed plant male meiocytes of Nicotiana tabacum and Secale cereale. To perform immunostaining or fluorescence in situ hybridization (FISH) on meiotic cells involved in cytomixis common protocols are modified. During preparation of specimens for subsequent cytological analysis, it is necessary not only to make DNA and proteins accessible to DNA probes and antibodies, but also to preserve cell cytoplasm. There are also some important modifications in the protocols applied for meiocytes of different plant species. Here we describe protocols for immunostaining and FISH in rigid tobacco male meiocytes with dense cytoplasm and thick callose wall, that tolerate hard squashing, and in soft rye male meiocytes, that are easily damaged upon squashing, both to study cytomixis.

Published
2020
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