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Purpose: The relationship between external load and creatine-kinase (CK) response at the team/position or individual level using Global Positioning Systems (GPS) has been studied. This study aimed to compare GPS-derived and Football Movement Profile (FMP) –derived CK-prediction models for national-team soccer players. The second aim was to compare the performance of general and individualized CK prediction models. Methods: Four hundred forty-four national-team soccer players (under 15 [U15] to senior) were monitored during training sessions and matches using GPS. CK was measured every morning from whole blood. The players had 19.3 (18.1) individual GPS-CK pairs, resulting in a total of 8570 data points. Machine learning models were built using (1) GPS-derived or (2) FMP-based parameters or (3) the combination of the 2 to predict the following days' CK value. The performance of general and individual-specific prediction models was compared. The performance of the models was described by R2 and the root-mean-square error (RMSE, in units per liter for CK values). Results: The FMP model (R2 =.60, RMSE = 144.6 U/L) performed similarly to the GPS-based model (R2 =.62, RMSE = 141.2 U/L) and the combination of the 2 (R2 =.62, RMSE = 140.3 U/L). The prediction power of the general model was better on average (R2 =.57 vs R2 =.37) and for 73% of the players than the individualized model. Conclusions: The results suggest that FMP-based CK-prediction models perform similarly to those based on GPS-derived metrics. General machine learning models' prediction power was higher than those of the individual-specific models. These findings can be used to monitor postmatch recovery strategies and to optimize weekly training periodization. [ABSTRACT FROM AUTHOR]

No studies have reported ground reaction force (GRF) profiles of the repeated depth jump (DJ) protocols commonly used to study exercise-induced muscle damage. Furthermore, while compression garments (CG) may accelerate recovery from exercise-induced muscle damage, any effects on the repeated bout effect are unknown. Therefore, we investigated the GRF profiles of 2 repeated bouts of damage-inducing DJs and the effects of wearing CG for recovery. Nonresistance-trained males randomly received CG (n = 9) or placebo (n = 8) for 72 hours recovery, following 20 × 20 m sprints and 10 × 10 DJs from 0.6 m. Exercise was repeated after 14 days. Using a 3-way (set × bout × group) design, changes in GRF were assessed with analysis of variance and statistical parametric mapping. Jump height, reactive strength, peak, and mean propulsive forces declined between sets (P <.001). Vertical stiffness, contact time, force at zero velocity, and propulsive duration increased (P <.05). According to statistical parametric mapping, braking (17%–25% of the movement) and propulsive forces (58%–81%) declined (P <.05). During the repeated bout, peak propulsive force and duration increased (P <.05), while mean propulsive force (P <.05) and GRF from 59% to 73% declined (P <.001). A repeated bout of DJs differed in propulsive GRF, without changes to the eccentric phase, or effects from CG. [ABSTRACT FROM AUTHOR]

This study examined the repeated bout effect (RBE) on muscle damage markers following two bouts of neuromuscular electrical stimulation (NMES) in untrained individuals. Following familiarization, participants received 45 consecutive NMES to the biceps brachii at an intensity that produced low evoked force for the elbow flexors. Muscle damage markers (maximal voluntary isometric contraction [MVIC], elbow range of motion [ROM], muscle soreness via visual analogue scale [VAS] scores, pressure pain threshold [PPT], and muscle thickness) were measured before (PRE), after (POST), 1 day after (24 POST), and 2 days after (48 POST) NMES. Following 1 week of rest, procedures were replicated. Separate repeated measures two-way ANOVAs examined each measure. There were no interactions or bout main effects for MVIC or ROM. Time main effects indicated PRE MVIC was greater than POST (p = 0.002) and 24-POST (p = 0.024), and PRE ROM was greater than POST (p = 0.036). There was no interaction for muscle thickness. Respective time and bout main effects indicated muscle thickness at PRE was less than POST (p = 0.017), and second-bout muscle thickness (p = 0.050) was less compared to the initial-bout. For PPT, there was an interaction (p = 0.019). Initial-bout PRE PPT was less than POST (p = 0.033). Initial-bout 48- POST PPT was less than second-bout 48-POST (p = 0.037). There was a significant interaction for VAS (p = 0.009). Initial-bout PRE VAS was less than POST (p = 0.033) and 24-POST (p = 0.015). Initial-bout POST and 24- POST VAS were greater than second-bout POST (p = 0.023) and 24-POST (p = 0.006), respectively. The results support RBE on muscle damage markers related to inflammation, but not MVIC and ROM. [ABSTRACT FROM AUTHOR]

The present investigation examined the influence of age and pubertal transition on magnitude of muscle damage and inflammatory response following high intensity incremental treadmill running till volitional exhaustion in sixty-four sedentary prepubertal (n = 32) and postpubertal (n = 32) boys who were randomly recruited in the study. Muscle damage and inflammatory markers like creatine kinase (CK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotranferase (AST), C--Reactive Protein (CRP) and Interleukin-6 (IL--6) were estimated before and after exercise. Serum CK, LDH, AST, ALT, CRP and IL--6 levels significantly increased after exercise in both the groups in comparison to respective pre--exercise values. Although CK, LDH, CRP and IL--6 responses were significantly higher in postpubertal boys, no intergroup variation was noted in post-exercise ALT activity. Age and body mass index (BMI) had significant positive correlation with post-exercise CK, LDH, AST, CRP, and IL--6 levels. Muscle injury and inflammation were significantly higher in postpubertal boys, suggesting a rise in these responses as a function of age and muscle mass during onset of puberty. Post--exercise release pattern of ALT was not influenced by age and puberty. Data also revealed that concentric endurance exercise did not induce extensive muscle damage and inflammation in both the groups. Drastic elevation in IL--6 level despite lower muscle damage implied that this marker was released from contracting muscle fibers independent of muscle damage and acute inflammation. The magnitude of this post--exercise IL--6 release increased as a function of age and BMI. [ABSTRACT FROM AUTHOR]

This study examined the impact of three pre-season fitness training protocols with different training frequencies (heavy, moderate and light) on the physical abilities and the biochemical indicators of muscle damage and oxidative stress in female basketball athletes as expressed through physiological and biochemical assessments. Also, a comparison and a search for correlations were made between the physiological and biochemical changes that were caused by each protocol. The sample consisted of 28 professional female basketball athletes, who competed in three different teams, and each training protocol was assigned to each of the three teams. The somatometric and performance markers measured were as follows: weight, fat percentage, aerobic capacity, anaerobic capacity, vertical jumping ability, speed, acceleration, explosiveness and maximum lower body part strength. The biochemical measurements consisted of exercise-induced muscle damage (creatine kinase—CK, lactate dehydrogenase—LDH) and oxidative stress markers (protein carbonyls—PCs, glutathione peroxidase—GPx). The three fitness training protocols that were used improved the fitness status of high-level female basketball athletes after 6 weeks during the pre-season period, but they were also accompanied by some significant differences between them (fat percentage, anaerobic capacity, speed and acceleration). The results were also accompanied by an increase in CK and LDH for the three teams and by an increase in GPx for Teams 1 and 3. Also, we concluded that even 4 more or 4 less fitness training units (TUs) during a 6-week basketball preparation period can be considered important to differentiate the outcomes of physiological and biochemical markers. The purpose of this study was to provide more theoretical and practical knowledge to basketball coaches and trainers, so they can optimize the training process during the preparation period and thus maximize the performance of their athletes. Nevertheless, the present study was created with certain limitations, such as the small sample size for each team, the absence of a long-term follow-up or the lack of a control group. Future studies may need to turn their focus on fitness protocols with longer durations, try to differentiate the types of training protocols and attempt to achieve a better balance between maximizing performance and mitigating the muscle damage and oxidative stress levels. [ABSTRACT FROM AUTHOR]

Background: In total hip arthroplasty (THA), soft tissue retraction is crucial, but traditional methods may cause damage. This study addresses the issue by introducing the Gripper Table Mounted System, a pulley-based retraction system. The research compares THA outcomes with and without the Gripper system, whether reducing soft tissue damage and postoperative pain. The Gripper, with its unique design, aims to minimize tissue damage during surgery. Materials and methods: The study conducted a retrospective analysis of 180 patients (180 hips) who underwent THA via an antero-lateral approach with the Gripper System or conventional retraction methods. Primary outcomes included gluteus medius cross-sectional area and hip abductor muscle strength. Secondary outcomes were pain VAS, intraoperative bleeding, operative time, laboratory data, and WOMAC score. Results: The study compared outcomes between those using the Gripper System (Gripper + group, n = 58) and those without (Gripper - group, n = 122). Both groups exhibited no significant differences in demographics or operative parameters. Gripper + group showed enhanced recovery in gluteus medius cross-sectional area and hip abductor muscle strength, with significant analgesia at various postoperative time points. No complications were noted in either group. Conclusions: The Gripper system proved effective in early analgesia, swift recovery of hip strength, and preserving muscle area. Its single-use, sterile, and compact design offers advantages over traditional retractor holders or human assistance, potentially reducing soft tissue damage and postoperative pain. This study concluded the Gripper system’s value in reducing pain and restoring strength in THA. Level of evidence: Therapeutic Level III. Trial registration: The University Hospital Medical Information Network (UMIN) registration number UMIN000052948. [ABSTRACT FROM AUTHOR]

Intracellular calcium ion regulation drives skeletal muscle adaptations posteccentric contractions: key role for ryanodine receptors. Eccentric contractions (ECC) induce excessive intracellular calcium ion (Ca2+) accumulation and muscle structural damage in localized regions of the muscle fibers. In this investigation, we present the novel hypothesis that the ryanodine receptor (RyR) plays a central role in evoking a Ca2+ dynamics profile that is markedly distinguishable from other muscle adaptive responses. [ABSTRACT FROM AUTHOR]

Eccentric contractions (ECC) are accompanied by the accumulation of intracellular calcium ions ([Ca2+]i) and induce skeletal muscle damage. Suppressed muscle damage in repeated bouts of ECC is well characterized; however, whether it is mediated by altered Ca2+ profiles remains unknown. We tested the hypothesis that repeated ECC suppresses Ca2+ accumulation via adaptations in Ca2+ regulation. Male Wistar rats were divided into two groups: ECC single bout (ECC-SB) and repeated bout (ECC-RB). Tibialis anterior (TA) muscles were subjected to ECC (40 times, 5 sets) once (ECC-SB) or twice 14 days apart (ECC-RB). Under anesthesia, the TA muscle was loaded with Ca2+ indicator Fura 2-AM, and the 340/380 nm ratio was evaluated as [Ca2+]i. Ca2+ handling proteins were measured by Western blots. ECC induced [Ca2+]i increase in both groups, but ECC-RB evinced a markedly suppressed [Ca2+]i (Time: P < 0.01, Group: P = 0.0357). Five hours post-ECC, in contrast to the localized [Ca2+]i accumulation in ECC-SB, ECC-RB exhibited lower and more uniform [Ca2+]i (P < 0.01). In ECC-RB, mitochondria Ca2+ uniporter complex (MCU) components MCU and MICU2 were significantly increased pre-second ECC bout (P < 0.01), and both SERCA1 and MICU1 were better preserved after contractions (P < 0.01). Fourteen days after novel ECC, skeletal muscle mitochondrial Ca2+ regulating proteins were elevated. Following subsequent ECC, [Ca2+]i accumulation and muscle damage were suppressed and SERCA1 and MICU1 preserved. These findings suggest that tolerance to a subsequent ECC bout is driven, at least in part, by enhanced mitochondrial and sarcoplasmic reticulum Ca2+ regulation. NEW & NOTEWORTHY: We demonstrated a reduced [Ca2+]i profile with suppressed muscle damage after a repeated bout of ECC in vivo: the ECC-induced immediate [Ca2+]i increase was suppressed and the persistence of increased [Ca2+]i with localized accumulation was diminished after repeated ECC. This effect occurred consonant with the upregulation of the mitochondrial Ca2+ uniporter complex and better preservation of SERCA1 and MICU1. These findings suggest that the mechanistic bases for repeated bout protection involve adaptation of Ca2+ regulation. [ABSTRACT FROM AUTHOR]

Abstract It remains unclear whether exercises leading to different outcomes of delayed onset muscle soreness (DOMS) may also elicit different skin temperature responses. The aim of this study was to determine whether different intensities and volumes of a single-joint exercise influence the DOMS and skin temperature measurements differently in healthy adults. Thirty-nine men and women were randomly assigned to three groups performing different exercise of different intensities and volumes (Exhaustion, Fatigue, Submaximal) to induce DOMS in the biceps brachii. DOMS (numeric pain rate scale, NPRS), pressure pain threshold (PPT) and skin temperature (infrared thermography, IRT) were measured on exercise day and 48 h later. The different exercises resulted in lower PPT responses 48 h after exercise and different DOMS reported across the different groups. Skin temperature outcomes did not differ following the different protocols. We found an increased minimum skin temperature 48 h after exercise in groups performing more intense exercises, but such differences were found in both exercised and non-exercised arms. Differently of PPT outcomes, pain reported depended on exercise intensity, and skin temperature 48 h after exercise could not show acute exercise adaptations. Skin temperature responses are contingent upon the characteristics of the participants rather than exercise intensity.

Abstract Supplementing with antioxidants may be one of the most efficient means of minimizing oxidative stress during workouts in obese individuals. The aim of this study is to identify the results after twelve weeks of CrossFit workouts combined with Spinach thylakoid extract on the levels of insulin resistance (insulin and Homeostatic Model Assessment of Insulin Resistance [HOMA-IR]), fasting blood sugar (FBS), malondialdehyde (MDA), creatine kinase (CK), lactate dehydrogenase (LDH), total antioxidant capacity (TAC), and superoxide dismutase (SOD), glutathione peroxidase (GPx) in obese males. Sixty-eight males with an average age of 27 ± 8 yrs and a BMI of 32.6 ± 2.6 kg.m− 2 were randomly split into four groups each consisting of seventeen individuals. : control group (CG), supplement group (SG), training group (TG), and training + supplement group (TSG). After initial assessments, the two training groups (TG and TSG) started on a 12 weeks of the CrossFit workouts program involving three sessions per week each lasting up to 60 min. Participants in supplement groups ingested 30 min before lunch, 5 gof Spinach thylakoid extract per day or one sachet of raw corn starch in the control group. Baseline and post-intervention measurements were performed 48 h pre- and post-last session, respectively. The findings revealed noteworthy relationships between the exercise groups and timefor TAC, SOD, GPx, MDA, CK, and LDH (p

Abstract Complement-dependent cytotoxicity (CDC) is a primary mechanism-of-action of monoclonal antibody (mAb) immunotherapies used to treat haematological cancers, including rituximab and daratumumab. However, mAb efficacy may be limited by reduced bioavailability of complement C1q – which activates the complement classical pathway following interactions with mAb-opsonised target cells. C1q is secreted by phagocytes upon recruitment to sites of muscle damage to facilitate muscular repair, hence we hypothesised that muscle damaging exercise may increase C1q ‘spill-over’ into blood. Additionally, other complement proteins (e.g., C1s) have been reported to increase following ultra-endurance and resistance exercise. Taken together, we hypothesised that muscle damaging exercise could be harnessed to enhance mAb-mediated CDC. In this study, n = 8 healthy males (28 ± 5-years) completed two 45-minute treadmill running protocols: (1) a flat running protocol at a speed 15% above anaerobic threshold, and (2) a downhill running protocol (− 10% slope) at the same speed. Blood samples were collected before, immediately after, and 1-hour, 24-hours, 2-days, and 4-days after exercise. Isolated serum was assessed for C1q by ELISA, and used to measure mAb (rituximab, daratumumab) mediated CDC against two haematological cancer cell lines (Raji, RPMI-8226) in vitro. Isolated plasma was assessed for markers of inflammation (C-reactive protein [CRP]), and muscle damage (creatine kinase [CK]) by turbidimetry. C1q and CDC activity were not different between running protocols and did not change over time (p > 0.05). Significantly greater perceived muscle soreness (p

Abstract Unaccustomed eccentric exercise (EE) is protective against muscle damage following a subsequent bout of similar exercise. One hypothesis suggests the existence of an alteration in motor unit (MU) behaviour during the second bout, which might contribute to the adaptive response. Accordingly, the present study investigated MU changes during repeated bouts of EE. During two bouts of exercise where maximal lengthening dorsiflexion (10 repetitions × 10 sets) was performed 3 weeks apart, maximal voluntary isometric torque (MVIC) and MU behaviour (quantified using high‐density electromyography; HDsEMG) were measured at baseline, during (after set 5), and post‐EE. The HDsEMG signals were decomposed into individual MU discharge timings, and a subset were tracked across each time point. MVIC was reduced similarly in both bouts post‐EE (Δ27 vs. 23%, P = 0.144), with a comparable amount of total work performed (∼1,300 J; P = 0.905). In total, 1,754 MUs were identified and the decline in MVIC was accompanied by a stepwise increase in discharge rate (∼13%; P

Abstract Background In the current study, we evaluated whether atopic dermatitis (AD) affects the entire body rather than being limited to skin barrier damage and inflammation. We hypothesized that medium-term exposure of distant organs to systemic inflammatory cytokines in sub-chronic inflammatory skin diseases has detrimental effects on distant tissues. Results Our findings demonstrated the dysregulation of genes and pathways associated with inflammation and the skin barrier, as well as genes and pathways involved in muscle development that respond to chemicals or stress in muscle tissues, all of which were reversed by hydrocortisone (Hc) administration. The expression of Ces1d showed significant differences during disease onset and after treatment in both skin and skeletal muscle, suggesting that Ces1d is likely responsible for the alleviation of subchronic AD. Conclusions Using NC/Nga mice with AD-like symptoms, we compared the transcriptomes of the skeletal muscle (a tissue that is relatively distant from the skin) with those of the skin (the lesion induction site) before and after disease induction, after which Hc was administered. Although further study is needed to better understand the effects of Ces1d on AD, skeletal muscle was associated with AD pathogenesis, and AD-like symptoms appeared to affect the body in a systemic manner. Given the importance of evidence-based medicine and the development of precision medicine, our findings provide insights into the mechanisms of AD onset and progression.

The Brazilian army recently developed the military physical training “Cross Operational” (CO), composed of four different difficulty levels. The moderate/high intensity character of CO can damage the muscle fibers, characterized by alterations at the cellular level, which can be evaluated indirectly by intramuscular proteins showing up in the bloodstream. In this context, the analysis of muscle damage biomarkers can be used as part of an initial injury prevention tool and in the evaluation of training progression and post-exercise recovery. This study aimed to observe the acute effect of CO on indirect markers of muscle damage in military personnel of the Brazilian army. Twenty-four male recruits aged between 18 and 26 years participated in the study. The four sessions corresponding to the CO levels were performed in cross-design, with a seven-day washout period, and blood samples were collected at rest, immediately after, 24 and 48 hours after each training session. The measured markers were creatine kinase (CK), myoglobin (Mb) and lactate dehydrogenase (LDH). In all CO levels, CK had a significant increase 24 hours after CO, whereas Mb and LDH incresead immediately after CO. Regarding recovery time, LDH returned to baseline values within 48 hours in all levels, whereas CK and Mb, after 48 hours of recovery, reduced but did not return to baseline in level 4. The serum elevations of the biomarkers studied, like those found in studies with other consolidated physical training methods, suggest that CO can contribute to the improvement of the physical training of military personnel.

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Introduction: Engaging in eccentric exercise can easily lead to significant muscle damage. Basketball, as an open-skill sport, involves many movements that require eccentric muscle actions. However, to the best of our knowledge, no previous study has systematically investigated whether the rest intervals between matches at different levels of basketball tournaments affect sports performance, contribute to cumulative fatigue, and increase injury risk from the perspective of player protection. Therefore, this study aimed to examine the above research questions. Methods: The latest schedule information was obtained from the official websites of three levels of basketball tournaments (e.g., 2021/2023 Women's/Men's National Amateur Basketball Championship; 2022/2023 Men's/Women's Basketball Asia Cup; 2022/2023 FIBA Basketball World Cup). Secondary data analysis was used to evaluate the rest intervals (in hours) between matches for all participating teams in each tournament. Relevant literature was reviewed and analyzed to infer the potential effects of short rest intervals on athletic performance, fatigue, and injury risk in these congested tournament schedules. Results: (1) Rest intervals between matches at different levels (combined for men's and women's): The average rest interval at the Taiwan level (28.2 ± 13.1 hours) was significantly shorter (p < .05) than at both the Asia level (42.8 ± 15.5 hours) and World level (49.0 ± 15.8 hours), with the Asia level also being significantly shorter than the World level. (2) Rest intervals by gender across levels: Rest intervals for men's matches were significantly longer (p < .05) than those for women's matches across all three tournament levels. (3) Rest intervals within each gender by level: For both men's and women's tournaments, the Taiwan level rest intervals were significantly shorter (p < .05) than those at the Asia and World levels, with the Asia level also being significantly shorter than the World level. (4) Literature findings: Previous studies have shown that a single basketball match can induce muscle damage lasting at least two days, and that congested schedules, or multiple matches increase the risk of injury and negatively impact athletic performance. Conclusion: These results indicate that the scheduling of rest intervals at all tournament levels does not sufficiently consider the impact on recovery, injury risk, and sports performance. Therefore, these findings will be presented to basketball tournament organizers as a reference for developing future guidelines and regulations. [ABSTRACT FROM AUTHOR]

BACKGROUND: Maca extract may regulate oxidative stress and muscle damage after exhaustive endurance exercise (EEE); however, its impact on these physiological activities remains obscure. OBJECTIVE: To examine the effects of Maca extract supplementation on oxidative stress, muscle damage and aerobic capacity after EEE. METHODS: Twenty healthy men were assigned to Maca or placebo groups and received two doses per day for 12 weeks. Pre- and post-EEE tests assessed levels of oxidative stress and muscle damage. The EEE test also recorded metrics such as time to exhaustion, peak oxygen consumption, and maximal and average heart rates. RESULTS: Post-exercise, there was no significant difference in oxidative stress markers between the Maca and placebo groups. However, the Maca group exhibited lower stress levels at both Post-4 and Post-24 in comparison to Post-0, while the placebo group only displayed a decrease at Post-4. Notably, Maca extract supplementation immediately increased catalase activity at Post-0. Though muscle damage markers did not significantly differ, the slope of CK elevation in the Maca group appeared lower than the Placebo group, suggesting Maca's potential in preventing muscle damage. However, no differences were noted on aerobic capacity markers. CONCLUSIONS: Maca extract may have some beneficial effects on reducing oxidative stress and possibly muscle damage after EEE. [ABSTRACT FROM AUTHOR]

Purpose: This study examined how a low dose of an eccentric-oriented lunge exercise could induce the repeated-bout effect (RBE) and affect the subsequent post-activation performance enhancement (PAPE) in recreational runners. Methods: Twenty male recreational runners (32.1 ± 2.8 years; 173.4 ± 6.1 cm; 73.3 ± 11.5 kg; 57.8 ± 7.2 mL·kg−1·min−1) were divided into control (N = 10) and experimental (N = 10) groups. In the first and fourth weeks, the groups were assessed for jump capacity, dynamic balance, and submaximal running kinematics before and after an incremental shuttle-run test until exhaustion. The experimental group was also submitted to two sessions of the eccentric-oriented lunge exercise (3 sets of 10 repetitions with 2 min of passive recovery) in the second and third weeks. Results: We observed that the first session promoted muscle damage, which was significantly (p <.05) reduced after the second training session, thus indicating an RBE. Meanwhile, there was no effect of the RBE on dynamic balance and submaximal running kinematics in the post-intervention. However, there was a significant increase in countermovement jump height (p =.008) for the experimental group when compared to the control group, although no PAPE was observed. Conclusions: The current results demonstrate that a simple, low-dose eccentric-oriented exercise may induce an RBE, leading to reduced muscle damage and a possibly improved lower limbs' muscle power in recreational runners. However, the absence of PAPE effects suggests that the RBE may not directly influence the potentiation/fatigue balance after fatiguing running exercises. [ABSTRACT FROM AUTHOR]

Curcumin is a natural polyphenolic compound exerting antioxidative and anti-inflammatory effects on various tissues, including muscles. Nevertheless, its clinical usage has been limited because of its poor bioavailability. Thus, highly bioavailable curcumin, such as Theracurmin (CR-033P) composed of 36% crystalline curcumin, has been developed. This study investigated the antioxidant activity and muscle-protective effect of Theracurmin Super (TS-P1), a new formulation with 36% amorphous curcumin. TS-P1 and CR-033P demonstrated antioxidant activity in vitro. Compared with pure curcumin, orally administered TS-P1 or CR-033P (16.7 or 50 mg per kg of body weight) at one-third dose inhibited the elevation of plasma lactate dehydrogenase and creatine kinase levels in a mouse downhill running model, indicating superior muscle-protective effect. Particularly, TS-P1 showed better or equivalent effectiveness in restoring the activity of antioxidant enzymes including glutathione peroxidase and catalase, and in suppressing inflammatory responses. Therefore, formulation with amorphous curcumin is a promising strategy for curcumin-based supplements. [ABSTRACT FROM AUTHOR]

Eccentric contraction (ECC) has been shown to induce leukocyte invasion into skeletal muscle, resulting in muscle inflammation. This study aimed to investigate whether prior ingestion of L-arginine (ARG), a nitric oxide precursor, inhibits ECC-induced macrophage invasion. Male Wistar rats received ARG in water for 7 days, beginning 3 days prior to ECC. ECCs were induced in the anterior crural muscles for 200 cycles. Three days later, the tibialis anterior and extensor digitorum longus muscles were excised for biochemical analysis and force measurement, respectively. ARG ingestion increased nitrite and nitrate levels in plasma and muscle, inhibiting force depression and reducing CD68 content in muscles subjected to ECC. ARG ingestion also ameliorated an ECC-induced increase in protein nitration, although neither ARG ingestion nor ECC induction affected protein carbonyl levels. The present results suggest that ingestion of ARG or ARG-rich foods may alleviate inflammation by attenuating phagocyte invasion in eccentrically contracted skeletal muscles. [ABSTRACT FROM AUTHOR]

N‐acetylcysteine (NAC) is a compound whose mechanism of action is intricately linked to the provision of cysteine for glutathione synthesis. It has been used in medicine and has also made significant inroads into sports, as it can modify the levels of several biomarkers, including those of oxidative processes, inflammation and muscle damage after exercise. Because the effectiveness of NAC supplementation is unclear, the primary objective of the present study was to perform a meta‐analysis elucidating how NAC supplementation alters the concentrations of GSH (glutathione), GSSG (glutathione disulfide), TBARS (thiobarbituric acid reactive substances), IL‐6 (interleukin 6), TNF‐α (tumour necrosis factor alpha), CK (creatine kinase), lactate, and muscle soreness after physical exertion. Suitable studies were searched for from February to September 2023, and the results of those included (n = 20) indicate that NAC supplementation significantly diminishes both muscle soreness (p = 0.03; the mean difference (MD) of NAC's effect was −0.43 with a 95% confidence interval (CI), −0.81, −0.04) and lactate concentrations after exercise (p = 0.03; the MD −0.56 mmol/L; 95% CI, −1.07, −0.06). A substantial decrease was observed in concentrations of IL‐6 (p = 0.03; the standardized MD (SMD) was −1.71; 95% CI, −3.26, −0.16) and TBARS (p = 0.02; SMD was −1.03, 95% CI, −1.90, −0.15). Furthermore, an elevation in GSH concentration was observed following supplementation. However, we saw no significant effect of NAC on TNF‐α, CK or GSSG concentrations. NAC supplementation holds promise for attenuating muscle soreness, lactate, TBARS and IL‐6 concentrations and increasing GSH level following physical exertion. [ABSTRACT FROM AUTHOR]

Complement-dependent cytotoxicity (CDC) is a primary mechanism-of-action of monoclonal antibody (mAb) immunotherapies used to treat haematological cancers, including rituximab and daratumumab. However, mAb efficacy may be limited by reduced bioavailability of complement C1q – which activates the complement classical pathway following interactions with mAb-opsonised target cells. C1q is secreted by phagocytes upon recruitment to sites of muscle damage to facilitate muscular repair, hence we hypothesised that muscle damaging exercise may increase C1q 'spill-over' into blood. Additionally, other complement proteins (e.g., C1s) have been reported to increase following ultra-endurance and resistance exercise. Taken together, we hypothesised that muscle damaging exercise could be harnessed to enhance mAb-mediated CDC. In this study, n = 8 healthy males (28 ± 5-years) completed two 45-minute treadmill running protocols: (1) a flat running protocol at a speed 15% above anaerobic threshold, and (2) a downhill running protocol (− 10% slope) at the same speed. Blood samples were collected before, immediately after, and 1-hour, 24-hours, 2-days, and 4-days after exercise. Isolated serum was assessed for C1q by ELISA, and used to measure mAb (rituximab, daratumumab) mediated CDC against two haematological cancer cell lines (Raji, RPMI-8226) in vitro. Isolated plasma was assessed for markers of inflammation (C-reactive protein [CRP]), and muscle damage (creatine kinase [CK]) by turbidimetry. C1q and CDC activity were not different between running protocols and did not change over time (p > 0.05). Significantly greater perceived muscle soreness (p < 0.001) and fluctuations observed from baseline to 24-hours post-exercise in the downhill running trial in CK (+ 171%) and CRP (+ 66%) suggests some degree of muscle damage was present. It is possible that any increase in C1q post-exercise may have been masked by the increase and subsequent interaction with CRP, which utilises C1q to facilitate muscular repair. This is the first study to investigate whether exercise can increase circulating C1q and improve mAb-mediated CDC and our findings show that downhill running exercise does not increase circulating C1q nor improve CDC in vitro. [ABSTRACT FROM AUTHOR]

Supplementing with antioxidants may be one of the most efficient means of minimizing oxidative stress during workouts in obese individuals. The aim of this study is to identify the results after twelve weeks of CrossFit workouts combined with Spinach thylakoid extract on the levels of insulin resistance (insulin and Homeostatic Model Assessment of Insulin Resistance [HOMA-IR]), fasting blood sugar (FBS), malondialdehyde (MDA), creatine kinase (CK), lactate dehydrogenase (LDH), total antioxidant capacity (TAC), and superoxide dismutase (SOD), glutathione peroxidase (GPx) in obese males. Sixty-eight males with an average age of 27 ± 8 yrs and a BMI of 32.6 ± 2.6 kg.m− 2 were randomly split into four groups each consisting of seventeen individuals. : control group (CG), supplement group (SG), training group (TG), and training + supplement group (TSG). After initial assessments, the two training groups (TG and TSG) started on a 12 weeks of the CrossFit workouts program involving three sessions per week each lasting up to 60 min. Participants in supplement groups ingested 30 min before lunch, 5 gof Spinach thylakoid extract per day or one sachet of raw corn starch in the control group. Baseline and post-intervention measurements were performed 48 h pre- and post-last session, respectively. The findings revealed noteworthy relationships between the exercise groups and timefor TAC, SOD, GPx, MDA, CK, and LDH (p < 0.001, ES: 0.88, 0.88, 0.8, 0.4, 0.7, and 0.7, respectively). In addition, there were statistically significant differences among study groups after attending the intervention program in TAC (ES: 0.88), SOD (ES: 0.92), GPX (ES: 0.85), MDA (ES: 0.5), CK (ES: 0.7) and LDH (ES: 0.8). The effect sizes of insulin (0.77), glucose (0.21), and HOMA-IR (0.44) varied significantly (p < 0.05) among the groups. The results demonstrated that CrossFit workouts for 12 weeks combined with Spinach thylakoid extract in men with obesity may prevent oxidative damage caused by obesity and CrossFit workouts. [ABSTRACT FROM AUTHOR]

Background: In the current study, we evaluated whether atopic dermatitis (AD) affects the entire body rather than being limited to skin barrier damage and inflammation. We hypothesized that medium-term exposure of distant organs to systemic inflammatory cytokines in sub-chronic inflammatory skin diseases has detrimental effects on distant tissues. Results: Our findings demonstrated the dysregulation of genes and pathways associated with inflammation and the skin barrier, as well as genes and pathways involved in muscle development that respond to chemicals or stress in muscle tissues, all of which were reversed by hydrocortisone (Hc) administration. The expression of Ces1d showed significant differences during disease onset and after treatment in both skin and skeletal muscle, suggesting that Ces1d is likely responsible for the alleviation of subchronic AD. Conclusions: Using NC/Nga mice with AD-like symptoms, we compared the transcriptomes of the skeletal muscle (a tissue that is relatively distant from the skin) with those of the skin (the lesion induction site) before and after disease induction, after which Hc was administered. Although further study is needed to better understand the effects of Ces1d on AD, skeletal muscle was associated with AD pathogenesis, and AD-like symptoms appeared to affect the body in a systemic manner. Given the importance of evidence-based medicine and the development of precision medicine, our findings provide insights into the mechanisms of AD onset and progression. [ABSTRACT FROM AUTHOR]

This systematic review synthesizes evidence on biomarker responses to physiological loads in professional male team sport athletes, providing insights into induced fatigue states. Structured searches across major databases yielded 28 studies examining various biomarkers in elite team sport players. Studies evaluated muscle damage markers, anabolic/catabolic hormones reflecting metabolic strain, inflammatory markers indicating immune activity and tissue damage, immunological markers tied to infection risk, and oxidative stress markers showing redox imbalances from excessive physiological load. Responses were examined in official matches and training across competitive seasons. The evidence shows that professional team sports induce significant alterations in all studied biomarkers, reflecting measurable physiological strain, muscle damage, oxidative stress, inflammation, and immunosuppression during intensive exercise. These effects tend to be larger and more prolonged after official matches compared to training. Reported recovery time courses range from 24-h to several days post-exercise. Monitoring biomarkers enables quantifying cumulative fatigue and physiological adaptations to training/competition loads, helping to optimize performance while mitigating injury and overtraining. Key biomarkers include creatine kinase, testosterone, cortisol, testosterone/cortisol ratio, salivary immunoglobulin-A, and markers of inflammation and oxidative stress. Further research should extend biomarker monitoring to cover psychological stress and affective states alongside physiological metrics for deeper insight into athlete wellness and readiness. [ABSTRACT FROM AUTHOR]

The purpose of this study was to examine the effects of menstrual cycle phase on myofiber injury, regenerative events, and inflammation after electrical stimulation (ES)-induced myofiber damage. Twenty-eight premenopausal women (20.8 ± 2 yr) were randomized into early follicular (EF; n = 14) and late follicular (LF; n = 14) groups. After menstrual cycle tracking and phase confirmation, subjects underwent 200 electrically stimulated eccentric muscle contractions 1 wk after providing a muscle biopsy. Seven days post-ES, subjects provided a final biopsy. Primary outcomes included serum estradiol, indirect markers of muscle damage, direct indicators of myofiber necrosis and regeneration, satellite cell number, and macrophage infiltration. Women in the LF group had higher serum estradiol (122.1 ± 23.4 vs. 81.7 ± 30.8 pg/mL; P < 0.001) than in the EF group on the day of ES. Although the EF group recovered baseline maximal isometric strength by 4 days post-ES, the LF group did not. Only women in the LF group showed significant and consistent evidence of myofiber necrosis and regeneration pre- to post-ES. Despite showing more evidence of myofiber damage, women in the LF group also experienced reduced total and CD206+ macrophage infiltration relative to the EF group. Satellite cell quantity increased significantly post-ES in both groups, with no differences between groups. Collectively, the data suggest that the high-estrogen LF phase may be associated with increased susceptibility to myofiber injury while also limiting the subsequent intramuscular inflammatory response. NEW & NOTEWORTHY: The menstrual cycle has widespread physiological effects across many systems, including skeletal muscle. In this study, we show that women in the late follicular phase of the menstrual cycle may be more susceptible to myofiber necrosis following electrical stimulation. We also show reduced evidence of inflammation in the late follicular phase. This is the first study to demonstrate a difference in the response of human skeletal muscle to a necrotic stimulus across a menstrual cycle. [ABSTRACT FROM AUTHOR]

Introduction: Skeletal muscle satellite cells (SC) contribute to the adaptive process of resistance exercise training (RET) and may be influenced by nutritional supplementation. However, little research exists on the impact of multi-ingredient supplementation on the SC response to RET. Purpose: We tested the effect of a multi-ingredient supplement (MIS) including whey protein, creatine, leucine, calcium citrate, and vitamin D on SC content and activity as well as myonuclear accretion, SC and myonuclear domain compared with a collagen control (COL) throughout a 10-wk RET program. Methods: Twenty-six participants underwent a 10-wk linear RET program while consuming either the MIS or COL supplement twice daily. Muscle biopsies were taken from the vastus lateralis at baseline and 48 h after a bout of damaging exercise, before and after RET. Muscle tissue was analyzed for SC and myonuclear content, domain, acute SC activation, and fiber cross-sectional area (fCSA). Results: MIS resulted in a greater increase in type II fCSA following 10 wk of RET (effect size (ES) = 0.89) but not myonuclear accretion or SC content. Change in myonuclei per fiber was positively correlated with type I and II and total fiber hypertrophy in the COL group only, indicating a robust independent effect of MIS on fCSA. Myonuclear domain increased similarly in both groups, whereas SC domain remained unchanged following RET. SC activation was similar between groups for all fiber types in the untrained state but showed a trend toward greater increases with MIS after RET (ES = 0.70). Conclusions: SC responses to acute damaging exercise and long-term RET are predominantly similar in MIS and COL groups. However, MIS can induce greater increases in type II fCSA with RET and potentially SC activation following damage in the trained state. [ABSTRACT FROM AUTHOR]

Purpose: This brief report aimed to characterize inter-individual training responses following a single session of high-intense whole-body electromyostimulation (WB-EMS) using markers of muscle damage over a period of 72 h. Methods: Twelve healthy individuals (5 men, 7 women; 32.0 ± 7 years) participated in a single 20-minute high-intensity WB-EMS training session. Markers of muscle damage, creatine kinase (CK) and myoglobin (Mb), were assessed before and immediately after training, as well as at 1.5, 3, 24, 48 and 72 h post-exercise. Lactate levels were determined pre- and post-exercise. Results: Overall, WB-EMS induced significant CK elevations, peaking at 72 h (18.358 ± 21.380 U/L; p < 0.01), and correlating Mb levels peaking at 48 h (1.509 ± 1.394 ng/dl, p < 0.01). Despite significant inter-individual variability in CK levels, both slow (SR) and fast responders (FR) were identified. FR showed significant increases in CK at all time points post WB-EMS (p < 0.05), whereas CK in SR significantly elevated after 48 h. Post-WB-EMS lactate concentration was identified to predict peak CK and Mb levels (r ≥ 0.65, both p < 0.05). Conclusion: High-intensity WB-EMS has the potential to induce severe muscle damage, as indicated by elevated levels of CK and Mb. We identified two distinct groups of individuals, SR and FR, indicating variability in response to WB-EMS. Furthermore, we suggest that individual responses to WB-EMS can be predicted based on post-WB-EMS lactate concentration. [ABSTRACT FROM AUTHOR]

Unaccustomed eccentric exercise (EE) is protective against muscle damage following a subsequent bout of similar exercise. One hypothesis suggests the existence of an alteration in motor unit (MU) behaviour during the second bout, which might contribute to the adaptive response. Accordingly, the present study investigated MU changes during repeated bouts of EE. During two bouts of exercise where maximal lengthening dorsiflexion (10 repetitions × 10 sets) was performed 3 weeks apart, maximal voluntary isometric torque (MVIC) and MU behaviour (quantified using high‐density electromyography; HDsEMG) were measured at baseline, during (after set 5), and post‐EE. The HDsEMG signals were decomposed into individual MU discharge timings, and a subset were tracked across each time point. MVIC was reduced similarly in both bouts post‐EE (Δ27 vs. 23%, P = 0.144), with a comparable amount of total work performed (∼1,300 J; P = 0.905). In total, 1,754 MUs were identified and the decline in MVIC was accompanied by a stepwise increase in discharge rate (∼13%; P < 0.001). A decrease in relative recruitment was found immediately after EE in Bout 1 versus baseline (∼16%; P < 0.01), along with reductions in derecruitment thresholds immediately after EE in Bout 2. The coefficient of variation of inter‐spike intervals was lower in Bout 2 (∼15%; P < 0.001). Our data provide new information regarding a change in MU behaviour during the performance of a repeated bout of EE. Importantly, such changes in MU behaviour might contribute, at least in part, to the repeated bout phenomenon. What is the central question of this study?Is motor unit (MU) behaviour altered during the performance of an initial and repeated bout of eccentric exercise (EE)?What is the main finding and its importance?During the repeated bout of exercise, we observed a greater contribution from lower threshold MUs to force generation along with reduced firing rate variability. Results suggest an adaptation occurs during the performance of an initial bout of exercise and contributes to the accelerated recovery that occurs following the performance of a repeated bout of EE. Importantly, our data suggest that changes in MU behaviour contribute, partly, to the repeated bout phenomenon immediately upon performing a repeated bout of eccentric activity. [ABSTRACT FROM AUTHOR]

This study aimed to compare the acute inflammatory response following high-intensity eccentric exercise between resistance-trained young and master athletes with similar performance levels. Resistance-trained young (n=8; 22±2 years) and master (n=8; 52±4 years) male athletes of a similar performance level performed a standardized high-intensity eccentric squat exercise protocol (10 sets of half-squats at 70% of 1-repetition maximum). The serum concentration of 20 biomarkers related to tissue damage, inflammation, remodeling, and repair was measured at baseline, immediately after exercise, and over a 72 h recovery period. Both groups experienced similar muscle damage as evidenced by a comparable increase in creatine kinase activity 24 h after exercise (p<0.001). Interleukin-6 (p=0.009) and growth hormone (p<0.001) increased immediately post-exercise in both groups. Monocyte chemoattractant protein-1 increased immediately post-exercise only in young athletes (p=0.003) and then decreased 24 h later. There were no significant differences for the remaining variables, including cell markers related to neutrophil/macrophage activation or pro/anti-inflammatory cytokines. Resistance-trained young and master athletes, matched for performance level, showed an overall similar inflammatory response to eccentric exercise, possibly reflecting regulatory mechanisms or immunological adaptations to chronic stimulation in master athletes. [ABSTRACT FROM AUTHOR]

Homer, KA, Cross, MR, and Helms, ER. A survey of resistance training practices among physique competitors during peak week. J Strength Cond Res 38(10): 1745–1752, 2024—Physique athletes are ranked by their on-stage presentation of muscle size, proportionality, and leanness. To acutely maximize muscle size, competitors manipulate resistance training (RT) variables in the days before the contest, commonly referred to as peak week (PW). Resistance training manipulations during PW may act synergistically with nutrition strategies such as carbohydrate loading. However, because little information exists on changes made to RT during PW, the purpose of this research was to determine the current practices of physique athletes and whether competitor characteristics were predictive of the RT variables manipulated. A total of 104 responses to the RT section of a survey on PW nutrition and training were analyzed through a series of multiple logistic regression models to examine the relationship between RT manipulations and competitor characteristics. Furthermore, to determine the magnitude of differences between PW and the week before PW (WBPW) for these variables, a McNemar-Bowker test, paired t-tests, and Wilcoxon signed-rank tests were conducted for nominal, continuous, and discrete outcomes, respectively. For all statistical analyses, p <0.05 was deemed significant. Competitors generally adjusted RT in a variety of ways, where proximity-to-failure was the most frequently manipulated and training frequency was the least; however, no competitor characteristic predicted any of the RT variables manipulated. Within those who manipulated RT variables during PW, frequency, volume, and intensity decreased while repetition ranges of compound exercises increased, empirically confirming that competitors seek to reduce training stress during PW. Such findings can be incorporated in future experimental designs examining the efficacy of peaking strategies to enhance the generalizability of results. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the effects of 2 plyometric training (PT) sessions on postural control (PC). Twelve physically active men (27.5 ± 6.41 years, 82.7 ± 13.0 kg, and 24.17 ± 8.87%F) performed PT in two moments (week 1 and week 3), with a 14-day recovery period. The PT consisted in 10 series of 10 CMJ jumps, with 1 min rest intervals. PC parameters were measured during 30 s, double-leg stance stabilometry trials with eyes open and closed on a force platform. Additionally, knee extensors and flexors maximal voluntary isometric contraction (MVIC) were evaluated at 90° and 70° knee joint positions using an isokinetic dynamometer (Biodex System 4 Pro, Biodex Medical Systems, United States) with participants seated at 85° hip flexion (0° = full hip extension). In both weeks, PC and MVIC were assessed before (Pre), and after 24, 48 and 72 h of the PT session. Significant changes were observed 24 h after the jump protocol for MVIC of the knee extensors (F = 3,66, P = 0.005). No changes during the recovery time were found for the MVIC of knee flexors. No week * time interactions were observed for any of the evaluated PC parameters (eyes open and closed). The PT used was able to reduce the MVIC of the knee extensors after 24 h,but failed to cause changes in PC parameters. [ABSTRACT FROM AUTHOR]

Background and study aim: It is common for students to engage in school competitions; however, these competitions typically do not allow for games to be played with intervals longer than 24 hours between them. The aim of the present study was to investigate the effects of consecutive futsal games on the physical performance of under-20 school athletes. Material and Methods: Ten school athletes (17.9 ± 1.2 years, 173 ± 9 cm, 63.0 ± 4.6 kg, and 21.2 ± 2.7 kg.m-2) participating in a school futsal competition were assessed on consecutive days, with a 24-hour interval between each game. Assessments were conducted before three games and 17 hours after the third game. The following variables were evaluated in this order: Total Quality of Recovery (TQR), reactive agility (RA) and non-reactive agility (NRA), 5 and 10-m sprint, and counter-movement jump. The internal load of the games was measured using the session Rating of Perceived Exertion (sRPE). Results: TQR decreased 17 hours after the third game. There was a decline in the performance of NRA with dribbling, NRA with touch, RA with touch, and in the 10-m sprint 17 hours after the third game. The sRPE was higher in the third game compared to the first game. There was no change in RA with dribbling, counter-movement jump, and 5-m sprint throughout the games. Conclusions: The results demonstrate that a sequence of three futsal games with intervals of only 24 hours impairs performance in agility and speed, decreases the perception of recovery, and increases the perception of perceived stress in the game. [ABSTRACT FROM AUTHOR]

ObjectiveThis network meta-analysis and systematic review evaluated the recovery impacts of varying cold water immersion (CWI) protocols on acute exercise-induced muscle damage.MethodsWe searched CNKI, PubMed, Cochrane Library, Web of Science, and Embase from January 2000 to September 2024 for randomized controlled trials examining CWI’s recovery effects on acute muscle damage. Data extraction, study screening, and risk of bias assessment were conducted independently by two reviewers. Analyses were performed using Stata 16.0.ResultsA total of 55 RCTs were included, with 42 reporting delayed onset muscle soreness (DOMS), 36 reporting jump performance (JUMP), and 30 reporting creatine kinase (CK) levels. Network meta-analysis showed that compared with the control group, MD-MT-CWI: Medium-duration medium-temperature cold water immersion (10–15 min, 11°C–15°C) [SMD = −1.45, 95%CI(-2.13, −0.77), P < 0.01] and MD-LT-CWI: Medium-duration low-temperature cold water immersion (10–15 min, 5°C–10°C) [SMD = −1.12, 95%CI(-1.78, −0.47), P = 0.01] significantly reduced DOMS; MD-LT-CWI (10–15 min, 5°C–10°C) [SMD = 0.48, 95%CI(0.20, 0.77), P = 0.01] and MD-MT-CWI (10–15 min, 11°C–15°C) [SMD = 0.42, 95%CI(0.15, 0.70), P = 0.02] significantly improved JUMP; MD-MT-CWI (10–15 min, 11°C–15°C) [SMD = −0.85, 95%CI(-1.36, −0.35), P = 0.01] and MD-LT-CWI (10–15 min, 5°C–10°C) [SMD = −0.90, 95%CI(-1.46, −0.34), P = 0.02] significantly reduced CK. Cumulative probability ranking showed that MD-LT-CWI (10–15 min, 5°C–10°C) was the most effective for improving JUMP and reducing CK, while MD-MT-CWI (10–15 min, 11°C–15°C) was the most effective for reducing DOMS.ConclusionDifferent dosages of cold water immersion (varying in duration and temperature) had different effects on recovery from acute exercise-induced muscle damage. We found that MD-LT-CWI (10–15 min, 5°C–10°C) was most effective for improving biochemical markers (CK) and neuromuscular recovery, while MD-MT-CWI (10–15 min, 11°C–15°C) was most effective for reducing muscle soreness. In practice, we recommend using MD-LT-CWI (10–15 min, 5°C–10°C) and MD-MT-CWI (10–15 min, 11°C–15°C) to reduce Exercise-induced muscle damage (EIMD). However, due to the limitations of the included studies, further high-quality studies are needed to verify these conclusions.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42024602359.

Introduction: To gain a healthy lifestyle, several physical active people do popular sports such as basketball, as their hobby. However, the different physiological responses between athletes and recreational players have not yet been understood. Objective: This study aims to analyze differences of antioxidants, free radical, inflammation, and muscle damage markers between professional athletes and recreational basketball players. Methodology: The subjects were professional athletes (PA, n=10) and recreational basketball players (RP, n=10). All subjects performed 5x5 basketball. The heart rate (HR) during the game was recorded. Glutathione peroxidase (GPX), F2-isoprostane, C-reactive protein (CRP), and lactate dehydrogenase (LDH) were analyzed. Results: It showed the PA had lower HRmax compared to RP group (p=0.001), while blood markers level between PA and RP groups was insignificant difference (p≥0.05). However, the CRP of professional athletes tended to decrease after the game, while the RP group had a small increase after 1 hour. Both groups showed a transient decrease in LDH after 1 hour. Interestingly, 1 hour after the games, RP experienced a slight decrease of F2-isoprostane level, while PA underwent a small decrease of GPX level. Conclusions: The professional athletes have better cardiovascular endurance. However, after 1 hour of the game, LDH, CRP, F2-IsoPs, and GPX seem similar in both groups. It indicates that recreational players have a good benefit as active people. Even though, the level trend of the markers in professional athletes is superior to recreational players. Nevertheless, further investigation is still needed to figure out the physiological response mechanism with more complete markers.

Purpose: To examine the effects of acute curcumin (CURC) supplementation on recovery from a soccer match in male professional players. Methods: In a randomized, placebo-controlled, crossover design, 11 players from the under-23 team of an English Premier League club (age 19 [1] y, body mass 79.4 [7.9] kg, height 180.8 [5.7] cm) consumed 500 mg of CURC or a control (medium-chain triglycerides) immediately and 12 and 36 hours after a 90-minute match. Countermovement jump height (CMJ), reactive strength index (RSI), delayed-onset muscle soreness (DOMS, 0–200 mm), and subjective well-being were measured before and 12, 36, and 60 hours postmatch. Global positioning systems measured external load during matches, and dietary intake was recorded across the testing period. Results: External load and dietary intake did not differ between conditions (P ≥.246). CURC attenuated deficits in CMJ (P ≤.004) and RSI (P ≤.001) and reduced DOMS (P ≤.004) at all postmatch time points (except 60 h post for RSI). The greatest difference between control and CURC was 12 hours post for CMJ (P <.001, 1.91 [4.40] cm, 95% CI, 1.25 to 2.57, g = 0.36) and RSI (P =.003, 0.40 [0.41] AU, 95% CI, 0.17 to 0.63, g = 0.90) and 36 hours post for DOMS (P <.001, 47 [23] mm, 95% CI, −67 to −27, g = 2.12). Conclusions: CURC intake <36 hours after a soccer match attenuated DOMS and muscle function deficits, suggesting that CURC may aid recovery in professional male soccer players. [ABSTRACT FROM AUTHOR]

Highlights: 1. This study elucidates the effect of L-citrulline supplementation on creatine kinase MM (CK-MM) expression post-eccentric exercise, offering novel insights into its potential role in mitigating muscle damage. 2. The findings demonstrate that L-citrulline administration significantly reduces CK-MM expression levels in skeletal muscle tissue, suggesting its therapeutic potential in enhancing muscle recovery and performance following exercise-induced damage. 3. This study contributes valuable insights into the multifaceted benefits of L-citrulline supplementation for supporting overall muscle health and performance by identifying its protective effects under different mechanisms, including improved blood flow, antioxidant activity, enhanced mitochondrial function, and promotion of muscle protein synthesis. Abstract Eccentric exercise often induces muscle injuries in athletes, resulting in impaired performance and prolonged recovery time. Creatine kinase MM (CK-MM) is a biomarker for assessing muscle damage, with elevated levels indicating injury. L-citrulline, an amino acid, has shown promise in enhancing performance and reducing recovery time. However, its specific effect on CK-MM remains unclear. This study utilized immunohistochemistry analysis to investigate the effect of L-citrulline supplementation on CK-MM expression post-eccentric exercise in male BALB/c mice. This in vivo study was conducted with a post-test-only design. A total of 25 mice were divided into two control groups (normal/C1 and negative/C2) and three treatment groups (T1, T2, and T3), each containing five mice. The T1, T2, and T3 groups were daily administered 250, 500, and 1,000 mg/kg bw of L-citrulline for seven days, respectively. All mice, except the C1 group, performed a downhill running procedure. The CK-MM expression in skeletal muscle tissue post-eccentric exercise was assessed using immunohistochemistry analysis. The statistical analysis included the Shapiro-Wilk test for data distribution and the Kruskal-Wallis and Mann-Whitney post-hoc tests for significant differences (p

The study examines the cardiac risks associated with certain herbal medicines compared to conventional allopathy drugs. Herbal remedies such as Citrus aurantium, Ephedra sinica, Aristolochia fangchi, Gly- cyrrhiza glabra, Corynanthe yohimbe, and Aconitum spp. are reviewed for their potential to cause adverse cardiac effects, including arrhythmias, hypertension, and cardiomyopathy. These risks are compared to allopathic drugs like digoxin, doxorubicin, and cyclophosphamide, which are known for their cardiotoxic profiles. Citrus aurantium and Ephedra sinica are noted for their stimulant properties that can lead to elevated blood pressure and increased heart rate, potentially resulting in cardiac arrhythmias. Aristolochia fangchi poses risks of nephrotoxicity and cardiotoxicity due to aristolochic acids. Glycyrrhiza glabra can cause hypokalemia, leading to arrhythmias, especially when used in high doses or for prolonged periods. Corynanthe yohimbe contains yohimbine, which may increase blood pressure and heart rate, heightening the risk of cardiovascular events. Aconitum spp., known for its potent alkaloids, can cause severe cardiac arrhythmias and hypotension. The study highlights the importance of awareness among healthcare providers and patients regarding the potential cardiac risks of both herbal and allopathic medications. It emphasizes the need for careful consideration of the patient’s cardiovascular status when prescribing or consuming these substances, as well as the necessity of rigorous clinical trials and pharmacovigilance to better understand and mitigate these risks.

Abstract A significant increase in circulating cell‐free DNA (cfDNA) occurs with physical exercise, which depends on the type of exertion and the duration. The aims of this study were as follows: (1) to investigate the time course of cfDNA and conventional markers of muscle damage from immediately after to 96 h after muscle‐damaging exercise; and (2) to investigate the relationship between cfDNA and indicators of primary (low‐frequency fatigue and maximal voluntary isometric contraction) and secondary (creatine kinase and delayed‐onset muscle soreness) muscle damage in young healthy males. Fourteen participants (age, 22 ± 2 years; weight, 84.4 ± 11.2 kg; height, 184.0 ± 7.4 cm) performed 50 intermittent drop jumps at 20 s intervals. We measured cfDNA and creatine kinase concentrations, maximal voluntary isometric contraction torque, low‐frequency fatigue and delayed‐onset muscle soreness before and at several time points up to 96 h after exercise. Plasma cfDNA levels increased from immediately postexercise until 72 h postexercise (P

Exertional rhabdomyolysis (ER) is a condition where muscle breakdown occurs after intense and unaccustomed exercise in healthy individuals. It is characterized by muscle pain, weakness, and myoglobinuria, potentially leading to acute kidney injury and worsening the patients’ prognosis. Ultra-trail races (UT) necessitate high energy and extreme muscular exertion, which can result in significant muscle breakdown, leading to ER and elevated biomarkers such as creatine kinase (CK) and lactate dehydrogenase (LDH). These races involve longer durations and both uphill and downhill elevations, with the latter causing more muscle damage. This systematic review aims to analyse the effect of downhill elevation (at least 1000 m) in UT on muscle and liver damage biomarkers. We conducted a systematic review of four electronic databases (Pubmed, Web of Science, Scopus, and Sportdiscus) based on PRISMA guidelines for systematic reviews. We included a total of 15 articles out of 6670 published between January 2005 and March 2024. The total population sample included 348 subjects, comprising 294 men (84.48%) and 54 women (15.52%) with a mean age of 39.82 ± 6.89 years. Only one subject (0.28%) was diagnosed with ER. The median increase in CK post vs. pre was 5370.63 ± 7289.71%, LDH post vs. pre was 311.20 ± 164.4%, and liver damage biomarkers aspartate transaminase (AST) and alanine aminotransferase (ALT) obtained mean increases of 1009.94 ± 743.97% and 207.02 ± 92.84%, respectively. No liver injury cases were reported. These findings suggest that ER is often misdiagnosed in UT and may result in acute kidney injury under certain circumstances. Therefore, it is crucial to define and prepare the characteristics required for ultra runners to safely participate in these extreme races.

Crush syndrome, which frequently occurs in earthquake disasters, often leads to rhabdomyolysis induced acute kidney injury (RIAKI). Recent findings indicate that systemic inflammatory response syndrome (SIRS) exacerbates muscle collapse, contributing to RIAKI. The purpose of this study is to investigate the involvement of multiple site inflammation, including intraperitoneal, in crush syndrome. In a mouse model of RIAKI, elevated levels of inflammatory mediators such as TNFα, IL-6, myoglobin, and dsDNA were observed in serum and the peritoneal cavity, peaking earlier in the intraperitoneal cavity than in serum or urine. Our previously developed novel peptide inhibiting leukocyte extracellular traps was administered intraperitoneally and blocked all of these mediators in the intraperitoneal cavity and serum, ameliorating muscle damage and consequent RIAKI. Although further studies are needed to determine whether intraperitoneal inflammation associated with muscle collapse can lead to systemic inflammation, resulting in more severe and prolonged muscle damage and renal injury, early suppression of multiple site inflammation, including intraperitoneal, might be an effective therapeutic target. [ABSTRACT FROM AUTHOR]

Introduction: The effect of eccentric exercise--induced muscle damage (EIMD) on cycling efficiency is unknown. The aim of the present study was to assess the effect of EIMD on gross and delta efficiency and the cardiopulmonary responses to cycle ergometry. Methods: Twenty-one recreational athletes performed cycling at 70%, 90%, and 110% of the gas exchange threshold (GET) under control conditions (Control) and 24 h following an eccentric damaging protocol (Damage). Knee extensor isometric maximal voluntary contraction, potentiated twitch (Qtw,pot), and voluntary activation were assessed before Control and Damage. Gross and delta efficiency were assessed using indirect calorimetry, and cardiopulmonary responses were measured at each power output. Electromyography root-mean-square (EMGRMS) during cycling was also determined. Results: Maximal voluntary contraction was 25%± 18%lower for Damage than Control (P < 0.001). Gross efficiency was lower for Damage than Control (P < 0.001) by 0.55% ± 0.79%, 0.59% ± 0.73%, and 0.60% ± 0.87% for 70%, 90%, and 110% GET, respectively. Delta efficiency was unchanged between conditions (P = 0.513). Concurrently, cycling EMGRMS was higher for Damage than Control (P = 0.004). An intensity-dependent increase in breath frequency and ... was found, which were higher for Damage only at 110% GET (P ≤ 0.019). Conclusions: Thus, gross efficiency is reduced following EIMD. The concurrently higher EMGRMS suggests that increases in muscle activation in the presence of EIMD might have contributed to reduced gross efficiency. The lack of change in delta efficiency might relate to its poor reliability hindering the ability to detect change. The findings also show that EIMD-associated hyperventilation is dependent on exercise intensity, which might relate to increases in central command with EIMD. [ABSTRACT FROM AUTHOR]

Exertional rhabdomyolysis (ER) is a condition where muscle breakdown occurs after intense and unaccustomed exercise in healthy individuals. It is characterized by muscle pain, weakness, and myoglobinuria, potentially leading to acute kidney injury and worsening the patients' prognosis. Ultra-trail races (UT) necessitate high energy and extreme muscular exertion, which can result in significant muscle breakdown, leading to ER and elevated biomarkers such as creatine kinase (CK) and lactate dehydrogenase (LDH). These races involve longer durations and both uphill and downhill elevations, with the latter causing more muscle damage. This systematic review aims to analyse the effect of downhill elevation (at least 1000 m) in UT on muscle and liver damage biomarkers. We conducted a systematic review of four electronic databases (Pubmed, Web of Science, Scopus, and Sportdiscus) based on PRISMA guidelines for systematic reviews. We included a total of 15 articles out of 6670 published between January 2005 and March 2024. The total population sample included 348 subjects, comprising 294 men (84.48%) and 54 women (15.52%) with a mean age of 39.82 ± 6.89 years. Only one subject (0.28%) was diagnosed with ER. The median increase in CK post vs. pre was 5370.63 ± 7289.71%, LDH post vs. pre was 311.20 ± 164.4%, and liver damage biomarkers aspartate transaminase (AST) and alanine aminotransferase (ALT) obtained mean increases of 1009.94 ± 743.97% and 207.02 ± 92.84%, respectively. No liver injury cases were reported. These findings suggest that ER is often misdiagnosed in UT and may result in acute kidney injury under certain circumstances. Therefore, it is crucial to define and prepare the characteristics required for ultra runners to safely participate in these extreme races. [ABSTRACT FROM AUTHOR]