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41 results on '"Muth, Günther"'

3. Mechanisms of Conjugative Transfer and Type IV Secretion-Mediated Effector Transport in Gram-Positive Bacteria

Author

Grohmann, Elisabeth, Keller, Walter, Muth, Günther, Compans, Richard W, Series Editor, Malissen, Bernard, Series Editor, Aktories, Klaus, Series Editor, Rappuoli, Rino, Series Editor, Galan, Jorge E., Series Editor, Ahmed, Rafi, Series Editor, Palme, Klaus, Series Editor, Casadevall, Arturo, Series Editor, Garcia-Sastre, Adolfo, Series Editor, Iwasaki, Akiko, Series Editor, Akira, Shizuo, Series Editor, Backert, Steffen, editor, and Grohmann, Elisabeth, editor

Published
2017
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20. Peptidoglycan Recycling in Gram-Positive Bacteria Is Crucial for Survival in Stationary Phase

Author

Borisova, Marina, primary, Gaupp, Rosmarie, additional, Duckworth, Amanda, additional, Schneider, Alexander, additional, Dalügge, Désirée, additional, Mühleck, Maraike, additional, Deubel, Denise, additional, Unsleber, Sandra, additional, Yu, Wenqi, additional, Muth, Günther, additional, Bischoff, Markus, additional, Götz, Friedrich, additional, and Mayer, Christoph, additional

Published
2016
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37. The stability region of the Streptomyces lividans plasmid pIJ101 encodes a DNA-binding protein recognizing a highly conserved short palindromic sequence motif.

Author

Thoma, Lina, Sepulveda, Edgardo, Latus, Annette, and Muth, Günther

Subjects
STREPTOMYCES lividans, BACTERIAL genomes, PLASMID replication, PLASMIDS, ANTIBIOTIC-producing organisms, PALINDROMIC DNA
Abstract

Conjugation is a driving force in the evolution and shaping of bacterial genomes. In antibiotic producing streptomycetes even small plasmids replicating via the rolling-circle mechanism are conjugative. Although they encode only genes involved in replication and transfer, the molecular function of most plasmid encoded proteins is unknown. In this work we show that the conjugative plasmid pIJ101 encodes an overlooked protein, SpdA2. We show that SpdA2 is a DNA binding protein which specifically recognizes a palindromic DNA sequence (sps). sps is localized within the spdA2 coding region and highly conserved in many Streptomyces plasmids. Elimination of the palindrome or deletion of spdA2 in plasmid pIJ303 did not interfere with conjugative plasmid transfer or pock formation, but affected segregational stability. [ABSTRACT FROM AUTHOR]

Published
2014
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39. Conjugative DNA transfer in Streptomyces by TraB: is one protein enough?

Author

Thoma, Lina and Muth, Günther

Subjects
*BACTERIAL conjugation, *GENE transfection, *STREPTOMYCES, *ANTIBIOTIC-producing organism genetics, *SOIL microbiology, *PLASMID genetics, *ADENOSINE triphosphatase
Abstract

Antibiotic-producing soil bacteria of the genus Streptomyces form a huge natural reservoir of antibiotic resistance genes for the dissemination within the soil community. Streptomyces plasmids encode a unique conjugative DNA transfer system clearly distinguished from classical conjugation involving a single-stranded DNA molecule and a type IV protein secretion system. Only a single plasmid-encoded protein, TraB, is sufficient to translocate a double-stranded DNA molecule into the recipient in Streptomyces matings. TraB is a hexameric pore-forming ATPase that resembles the chromosome segregator protein FtsK and translocates DNA by recognizing specific 8-bp repeats present in the plasmid clt locus. Mobilization of chromosomal genes does not require integration of the plasmid, because TraB also recognizes clt-like sequences distributed all over the chromosome. [ABSTRACT FROM AUTHOR]

Published
2012
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40. Streptomyces ghanaensisPlasmid pSG5: Nucleotide Sequence Analysis of the Self-Transmissible Minimal Replicon and Characterization of the Replication Mode

Author

Muth, Günther, Farr, Martin, Hartmann, Vera, and Wohlleben, Wolfgang

Abstract

The naturally temperature-sensitive plasmid pSG5 of Streptomyces ghanaensisDSM 2932 is the basis replicon of the "pGM-vectors." The nucleotide sequence of the pSG5 minimal replicon was determined. Only one single open reading frame (rep) with high coding probability is located on the minimal replicon. The deduced Rep protein consists of 378 aa and contains motifs characteristic of initiation proteins for rolling-circle-type replication. Sequence similarity indicated that the Rep protein of pSG5 is related to the Rep proteins of the pC194 plasmid family. Accumulation of large amounts of single-stranded plasmid DNA was shown for all small pGM vectors. A minus origin for the lagging strand synthesis was localized outside of the pSG5 minimal replicon. Although the sequenced pSG5 fragment is self-transmissible, it seems not to carry further genes in addition to the repgene. This suggests that the transfer mechanism of plasmid pSG5 differs from that of other Streptomycesplasmids, which all encode specific transfer genes.

Published
1995
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41. Hopanoids are formed during transition from substrate to aerial hyphae in Streptomyces coelicolorA3(2)

Author

Poralla, Karl, Muth, Günther, and Härtner, Thomas

Abstract

Streptomyces coelicolorA3(2) contains a cluster of putative isoprenoid and hopanoid biosynthetic genes. The strain does not produce the pentacyclic hopanoids in liquid culture but produces them on solid medium when sporulating. Mutants defective in the formation of aerial mycelium and spores (bld), with the exception of bldB, do not synthesize hopanoids, whereas mutants, which form aerial mycelium but no spores (whi), do. The membrane condensing hopanoids possibly may alleviate stress in aerial mycelium by diminishing water permeability across the membrane.

Published
2000
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