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2. Flexible and cost-effective genomic surveillance of P. falciparum malaria with targeted nanopore sequencing

Author

de Cesare, Mariateresa, Mwenda, Mulenga, Jeffreys, Anna E., Chirwa, Jacob, Drakeley, Chris, Schneider, Kammerle, Mambwe, Brenda, Glanz, Karolina, Ntalla, Christina, Carrasquilla, Manuela, Portugal, Silvia, Verity, Robert J., Bailey, Jeffrey A., Ghinai, Isaac, Busby, George B., Hamainza, Busiku, Hawela, Moonga, Bridges, Daniel J., and Hendry, Jason A.

Published
2024
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4. Detection of Human Adenovirus and Rotavirus in Wastewater in Lusaka, Zambia: Demonstrating the Utility of Environmental Surveillance for the Community

Author

Saasa, Ngonda, primary, M’kandawire, Ethel, additional, Ndebe, Joseph, additional, Mwenda, Mulenga, additional, Chimpukutu, Fred, additional, Mukubesa, Andrew Nalishuwa, additional, Njobvu, Fred, additional, Shempela, Doreen Mainza, additional, Sikalima, Jay, additional, Chiyesu, Carol, additional, Muvwanga, Bruce, additional, Nampokolwe, Sarah M., additional, Sulwe, Clement, additional, Khondiwa, Thokozile, additional, Jennings, Todd, additional, Kamanga, Ameck, additional, Simulundu, Edgar, additional, Mulube, Conceptor, additional, Mwasinga, Wizaso, additional, Mumeka, Jalaimo, additional, Simwanza, John, additional, Sakubita, Patrick, additional, Kapona, Otridah, additional, Mulenga, Chilufya Susan Aneta, additional, Chipoya, Musole, additional, Musonda, Kunda, additional, Kapata, Nathan, additional, Sinyange, Nyambe, additional, Kapina, Muzala, additional, Siwila, Joyce, additional, Shawa, Misheck, additional, Kajihara, Masahiro, additional, Takada, Ayato, additional, Sawa, Hirofumi, additional, Choonga, Simulyamana A., additional, Chilengi, Roma, additional, Muyunda, Earnest, additional, Nalubamba, King S., additional, and Hang’ombe, Bernard M., additional

Published
2024
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5. Diversity and selection analyses identify transmission-blocking antigens as the optimal vaccine candidates in Plasmodium falciparum

Author

Ciubotariu, Ilinca I., primary, Broyles, Bradley K., additional, Xie, Shaojun, additional, Thimmapuram, Jyothi, additional, Mwenda, Mulenga C., additional, Mambwe, Brenda, additional, Mulube, Conceptor, additional, Matoba, Japhet, additional, Schue, Jessica L., additional, Moss, William J., additional, Bridges, Daniel J., additional, Qixin, He, additional, and Carpi, Giovanna, additional

Published
2024
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10. Flexible and cost-effective genomic surveillance ofP. falciparummalaria with targeted nanopore sequencing

Author

de Cesare, Mariateresa, primary, Mwenda, Mulenga, additional, Jeffreys, Anna E., additional, Chirwa, Jacob, additional, Drakeley, Chris, additional, Schneider, Kammerle, additional, Ghinai, Isaac, additional, Busby, George B., additional, Hamainza, Busiku, additional, Hawela, Moonga, additional, Bridges, Daniel J., additional, and Hendry, Jason A., additional

Published
2023
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11. Reactive focal drug administration associated with decreased malaria transmission in an elimination setting: Serological evidence from the cluster-randomized CoRE study

Author

Bridges, Daniel J., primary, Miller, John M., additional, Chalwe, Victor, additional, Moonga, Hawela, additional, Hamainza, Busiku, additional, Steketee, Richard W., additional, Mambwe, Brenda, additional, Mulube, Conceptor, additional, Wu, Lindsey, additional, Tetteh, Kevin K. A., additional, Drakeley, Chris, additional, Chishimba, Sandra, additional, Mwenda, Mulenga, additional, Silumbe, Kafula, additional, and Larsen, David A., additional

Published
2022
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12. What is the prevalence of COVID-19 detection by PCR among deceased individuals in Lusaka, Zambia? A postmortem surveillance study

Author

Gill, Christopher J, primary, Mwananyanda, Lawrence, additional, MacLeod, William B, additional, Kwenda, Geoffrey, additional, Pieciak, Rachel C, additional, Etter, Lauren, additional, Bridges, Daniel, additional, Chikoti, Chilufya, additional, Chirwa, Sarah, additional, Chimoga, Charles, additional, Forman, Leah, additional, Katowa, Ben, additional, Lapidot, Rotem, additional, Lungu, James, additional, Matoba, Japhet, additional, Mwinga, Gift, additional, Mubemba, Benjamin, additional, Mupila, Zachariah, additional, Muleya, Walter, additional, Mwenda, Mulenga, additional, Ngoma, Benard, additional, Nakazwe, Ruth, additional, Nzara, Diana, additional, Pawlak, Natalie, additional, Pemba, Lillian, additional, Saasa, Ngonda, additional, Simulundu, Edgar, additional, Yankonde, Baron, additional, and Thea, Donald M, additional

Published
2022
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13. The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

Author

Tegally, Houriiyah, primary, San, James E., additional, Cotten, Matthew, additional, Tegomoh, Bryan, additional, Mboowa, Gerald, additional, Martin, Darren P., additional, Baxter, Cheryl, additional, Moir, Monika, additional, Lambisia, Arnold, additional, Diallo, Amadou, additional, Amoako, Daniel G., additional, Diagne, Moussa M., additional, Sisay, Abay, additional, Zekri, Abdel-Rahman N., additional, Barakat, Abdelhamid, additional, Gueye, Abdou Salam, additional, Sangare, Abdoul K., additional, Ouedraogo, Abdoul-Salam, additional, Sow, Abdourahmane, additional, Musa, Abdualmoniem O., additional, Sesay, Abdul K., additional, Lagare, Adamou, additional, Kemi, Adedotun-Sulaiman, additional, Abar, Aden Elmi, additional, Johnson, Adeniji A., additional, Fowotade, Adeola, additional, Olubusuyi, Adewumi M., additional, Oluwapelumi, Adeyemi O., additional, Amuri, Adrienne A., additional, Juru, Agnes, additional, Ramadan, Ahmad Mabrouk, additional, Kandeil, Ahmed, additional, Mostafa, Ahmed, additional, Rebai, Ahmed, additional, Sayed, Ahmed, additional, Kazeem, Akano, additional, Balde, Aladje, additional, Christoffels, Alan, additional, Trotter, Alexander J., additional, Campbell, Allan, additional, Keita, Alpha Kabinet, additional, Kone, Amadou, additional, Bouzid, Amal, additional, Souissi, Amal, additional, Agweyu, Ambrose, additional, Gutierrez, Ana V., additional, Page, Andrew J., additional, Yadouleton, Anges, additional, Vinze, Anika, additional, Happi, Anise N., additional, Chouikha, Anissa, additional, Iranzadeh, Arash, additional, Maharaj, Arisha, additional, Batchi-Bouyou, Armel Landry, additional, Ismail, Arshad, additional, Sylverken, Augustina, additional, Goba, Augustine, additional, Femi, Ayoade, additional, Sijuwola, Ayotunde Elijah, additional, Ibrahimi, Azeddine, additional, Marycelin, Baba, additional, Salako, Babatunde Lawal, additional, Oderinde, Bamidele S., additional, Bolajoko, Bankole, additional, Dhaala, Beatrice, additional, Herring, Belinda L., additional, Tsofa, Benjamin, additional, Mvula, Bernard, additional, Njanpop-Lafourcade, Berthe-Marie, additional, Marondera, Blessing T., additional, Khaireh, Bouh Abdi, additional, Kouriba, Bourema, additional, Adu, Bright, additional, Pool, Brigitte, additional, McInnis, Bronwyn, additional, Brook, Cara, additional, Williamson, Carolyn, additional, Anscombe, Catherine, additional, Pratt, Catherine B., additional, Scheepers, Cathrine, additional, Akoua-Koffi, Chantal G., additional, Agoti, Charles N., additional, Loucoubar, Cheikh, additional, Onwuamah, Chika Kingsley, additional, Ihekweazu, Chikwe, additional, Malaka, Christian Noël, additional, Peyrefitte, Christophe, additional, Omoruyi, Chukwuma Ewean, additional, Rafaï, Clotaire Donatien, additional, Morang’a, Collins M., additional, Nokes, D. James, additional, Lule, Daniel Bugembe, additional, Bridges, Daniel J., additional, Mukadi-Bamuleka, Daniel, additional, Park, Danny, additional, Baker, David, additional, Doolabh, Deelan, additional, Ssemwanga, Deogratius, additional, Tshiabuila, Derek, additional, Bassirou, Diarra, additional, Amuzu, Dominic S.Y., additional, Goedhals, Dominique, additional, Grant, Donald S., additional, Omuoyo, Donwilliams O., additional, Maruapula, Dorcas, additional, Wanjohi, Dorcas Waruguru, additional, Foster-Nyarko, Ebenezer, additional, Lusamaki, Eddy K., additional, Simulundu, Edgar, additional, Ong’era, Edidah M., additional, Ngabana, Edith N., additional, Abworo, Edward O., additional, Otieno, Edward, additional, Shumba, Edwin, additional, Barasa, Edwine, additional, Ahmed, El Bara, additional, Kampira, Elizabeth, additional, Fahime, Elmostafa El, additional, Lokilo, Emmanuel, additional, Mukantwari, Enatha, additional, Cyril, Erameh, additional, Philomena, Eromon, additional, Belarbi, Essia, additional, Simon-Loriere, Etienne, additional, Anoh, Etilé A., additional, Leendertz, Fabian, additional, Taweh, Fahn M., additional, Wasfi, Fares, additional, Abdelmoula, Fatma, additional, Takawira, Faustinos T., additional, Derrar, Fawzi, additional, Ajogbasile, Fehintola V, additional, Treurnicht, Florette, additional, Onikepe, Folarin, additional, Ntoumi, Francine, additional, Muyembe, Francisca M., additional, Ngiambudulu, Francisco, additional, Zongo Ragomzingba, Frank Edgard, additional, Dratibi, Fred Athanasius, additional, Iyanu, Fred-Akintunwa, additional, Mbunsu, Gabriel K., additional, Thilliez, Gaetan, additional, Kay, Gemma L., additional, Akpede, George O., additional, George, Uwem E., additional, van Zyl, Gert, additional, Awandare, Gordon A., additional, Schubert, Grit, additional, Maphalala, Gugu P., additional, Ranaivoson, Hafaliana C., additional, Lemriss, Hajar, additional, Omunakwe, Hannah E, additional, Onywera, Harris, additional, Abe, Haruka, additional, Karray, Hela, additional, Nansumba, Hellen, additional, Triki, Henda, additional, Adje Kadjo, Herve Albéric, additional, Elgahzaly, Hesham, additional, Gumbo, Hlanai, additional, mathieu, Hota, additional, Kavunga-Membo, Hugo, additional, Smeti, Ibtihel, additional, Olawoye, Idowu B., additional, Adetifa, Ifedayo, additional, Odia, Ikponmwosa, additional, Boubaker, Ilhem Boutiba-Ben, additional, Ssewanyana, Isaac, additional, Wurie, Isatta, additional, Konstantinus, Iyaloo S, additional, Afiwa Halatoko, Jacqueline Wemboo, additional, Ayei, James, additional, Sonoo, Janaki, additional, Lekana-Douki, Jean Bernard, additional, Makangara, Jean-Claude C., additional, Tamfum, Jean-Jacques M., additional, Heraud, Jean-Michel, additional, Shaffer, Jeffrey G., additional, Giandhari, Jennifer, additional, Musyoki, Jennifer, additional, Uwanibe, Jessica N., additional, Bhiman, Jinal N., additional, Yasuda, Jiro, additional, Morais, Joana, additional, Mends, Joana Q., additional, Kiconco, Jocelyn, additional, Sandi, John Demby, additional, Huddleston, John, additional, Odoom, John Kofi, additional, Morobe, John M., additional, Gyapong, John O., additional, Kayiwa, John T., additional, Okolie, Johnson C., additional, Xavier, Joicymara Santos, additional, Gyamfi, Jones, additional, Kofi Bonney, Joseph Humphrey, additional, Nyandwi, Joseph, additional, Everatt, Josie, additional, Farah, Jouali, additional, Nakaseegu, Joweria, additional, Ngoi, Joyce M., additional, Namulondo, Joyce, additional, Oguzie, Judith U., additional, Andeko, Julia C., additional, Lutwama, Julius J., additional, O’Grady, Justin, additional, Siddle, Katherine J, additional, Victoir, Kathleen, additional, Adeyemi, Kayode T., additional, Tumedi, Kefentse A., additional, Carvalho, Kevin Sanders, additional, Mohammed, Khadija Said, additional, Musonda, Kunda G., additional, Duedu, Kwabena O., additional, Belyamani, Lahcen, additional, Fki-Berrajah, Lamia, additional, Singh, Lavanya, additional, Biscornet, Leon, additional, de Oliveira Martins, Leonardo, additional, Chabuka, Lucious, additional, Olubayo, Luicer, additional, Deng, Lul Lojok, additional, Ochola-Oyier, Lynette Isabella, additional, Mine, Madisa, additional, Ramuth, Magalutcheemee, additional, Mastouri, Maha, additional, ElHefnawi, Mahmoud, additional, Mbanne, Maimouna, additional, Matsheka, Maitshwarelo I., additional, Kebabonye, Malebogo, additional, Diop, Mamadou, additional, Momoh, Mambu, additional, Lima Mendonça, Maria da Luz, additional, Venter, Marietjie, additional, Paye, Marietou F, additional, Faye, Martin, additional, Nyaga, Martin M., additional, Mareka, Mathabo, additional, Damaris, Matoke-Muhia, additional, Mburu, Maureen W., additional, Mpina, Maximillian, additional, Claujens Chastel, Mfoutou Mapanguy, additional, Owusu, Michael, additional, Wiley, Michael R., additional, Tatfeng, Mirabeau Youtchou, additional, Ayekaba, Mitoha Ondo’o, additional, Abouelhoda, Mohamed, additional, Beloufa, Mohamed Amine, additional, Seadawy, Mohamed G, additional, Khalifa, Mohamed K., additional, Dellagi, Mohammed Koussai, additional, Matobo, Mooko Marethabile, additional, Kane, Mouhamed, additional, Ouadghiri, Mouna, additional, Salou, Mounerou, additional, Mbulawa, Mphaphi B., additional, Saibu, Mudashiru Femi, additional, Mwenda, Mulenga, additional, Kaba, Muluken, additional, Phan, My V.T., additional, Abid, Nabil, additional, Touil, Nadia, additional, Rujeni, Nadine, additional, Ismael, Nalia, additional, Top, Ndeye Marieme, additional, Dia, Ndongo, additional, Mabunda, Nédio, additional, Hsiao, Nei-yuan, additional, Silochi, Nelson Boricó, additional, Saasa, Ngonda, additional, Bbosa, Nicholas, additional, Murunga, Nickson, additional, Gumede, Nicksy, additional, Wolter, Nicole, additional, Sitharam, Nikita, additional, Ndodo, Nnaemeka, additional, Ajayi, Nnennaya A., additional, Tordo, Noël, additional, Mbhele, Nokuzola, additional, Razanajatovo, Norosoa H, additional, Iguosadolo, Nosamiefan, additional, Mba, Nwando, additional, Kingsley, Ojide C., additional, Sylvanus, Okogbenin, additional, Peter, Okokhere, additional, Femi, Oladiji, additional, Testimony, Olumade, additional, Ogunsanya, Olusola Akinola, additional, Fakayode, Oluwatosin, additional, Ogah, Onwe E., additional, Faye, Ousmane, additional, Smith-Lawrence, Pamela, additional, Ondoa, Pascale, additional, Combe, Patrice, additional, Nabisubi, Patricia, additional, Semanda, Patrick, additional, Oluniyi, Paul E., additional, Arnaldo, Paulo, additional, Quashie, Peter Kojo, additional, Bejon, Philip, additional, Dussart, Philippe, additional, Bester, Phillip A., additional, Mbala, Placide K., additional, Kaleebu, Pontiano, additional, Abechi, Priscilla, additional, El-Shesheny, Rabeh, additional, Joseph, Rageema, additional, Aziz, Ramy Karam, additional, Essomba, René Ghislain, additional, Ayivor-Djanie, Reuben, additional, Njouom, Richard, additional, Phillips, Richard O., additional, Gorman, Richmond, additional, Kingsley, Robert A., additional, Audu, Rosemary, additional, Carr, Rosina A.A., additional, Kabbaj, Saâd El, additional, Gargouri, Saba, additional, Masmoudi, Saber, additional, Sankhe, Safietou, additional, Mohamed, Sahra Isse, additional, Mhalla, Salma, additional, Hosch, Salome, additional, Kassim, Samar Kamal, additional, Metha, Samar, additional, Trabelsi, Sameh, additional, Lemriss, Sanaâ, additional, Agwa, Sara Hassan, additional, Mwangi, Sarah Wambui, additional, Doumbia, Seydou, additional, Makiala-Mandanda, Sheila, additional, Aryeetey, Sherihane, additional, Ahmed, Shymaa S., additional, Ahmed, Sidi Mohamed, additional, Elhamoumi, Siham, additional, Moyo, Sikhulile, additional, Lutucuta, Silvia, additional, Gaseitsiwe, Simani, additional, Jalloh, Simbirie, additional, Andriamandimby, Soafy, additional, Oguntope, Sobajo, additional, Grayo, Solène, additional, Lekana-Douki, Sonia, additional, Prosolek, Sophie, additional, Ouangraoua, Soumeya, additional, van Wyk, Stephanie, additional, Schaffner, Stephen F., additional, Kanyerezi, Stephen, additional, Ahuka-Mundeke, Steve, additional, Rudder, Steven, additional, Pillay, Sureshnee, additional, Nabadda, Susan, additional, Behillil, Sylvie, additional, Budiaki, Sylvie L., additional, van der Werf, Sylvie, additional, Mashe, Tapfumanei, additional, Aanniz, Tarik, additional, Mohale, Thabo, additional, Le-Viet, Thanh, additional, Velavan, Thirumalaisamy P., additional, Schindler, Tobias, additional, Maponga, Tongai, additional, Bedford, Trevor, additional, Anyaneji, Ugochukwu J., additional, Chinedu, Ugwu, additional, Ramphal, Upasana, additional, Enouf, Vincent, additional, Nene, Vishvanath, additional, Gorova, Vivianne, additional, Roshdy, Wael H., additional, Karim, Wasim Abdul, additional, Ampofo, William K., additional, Preiser, Wolfgang, additional, Choga, Wonderful T., additional, Ahmed, Yahaya Ali, additional, Ramphal, Yajna, additional, Bediako, Yaw, additional, Naidoo, Yeshnee, additional, Butera, Yvan, additional, de Laurent, Zaydah R., additional, Ouma, Ahmed E.O., additional, von Gottberg, Anne, additional, Githinji, George, additional, Moeti, Matshidiso, additional, Tomori, Oyewale, additional, Sabeti, Pardis C., additional, Sall, Amadou A., additional, Oyola, Samuel O., additional, Tebeje, Yenew K., additional, Tessema, Sofonias K., additional, de Oliveira, Tulio, additional, Happi, Christian, additional, Lessells, Richard, additional, Nkengasong, John, additional, and Wilkinson, Eduan, additional

Published
2022
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14. Sustained high prevalence of COVID-19 deaths from a systematic post-mortem study in Lusaka, Zambia: one year later

Author

Gill, Christopher J, primary, Mwananyanda, Lawrence, additional, MacLeod, William, additional, Kwenda, Geoffrey, additional, Pieciak, Rachel, additional, Etter, Lauren E, additional, Bridges, Daniel, additional, Chikot, Chilufya, additional, Chirwa, Sarah, additional, Chimoga, Charles, additional, Forman, Leah, additional, Katowa, Benjamin, additional, Lapidot, Rotem, additional, Lungu, James, additional, Matoba, Japhet, additional, Mwinga, Gift, additional, Mubemba, Benjamin, additional, Mupila, Zachariah, additional, Muleya, Walter, additional, Mwenda, Mulenga, additional, Ngoma, Benard, additional, Nakazwe, Ruth, additional, Nzara, Diana, additional, Pawlak, Natalie, additional, Pemba, Lilian, additional, Saasa, Ngonda, additional, Simulundu, Edgar, additional, Yankonde, Baron, additional, and Thea, Donald M, additional

Published
2022
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15. A year of genomic surveillance reveals how the SARS-CoV-2 pandemic unfolded in Africa

Author

Wilkinson, Eduan, primary, Giovanetti, Marta, additional, Tegally, Houriiyah, additional, San, James E., additional, Lessells, Richard, additional, Cuadros, Diego, additional, Martin, Darren P., additional, Rasmussen, David A., additional, Zekri, Abdel-Rahman N., additional, Sangare, Abdoul K., additional, Ouedraogo, Abdoul-Salam, additional, Sesay, Abdul K., additional, Priscilla, Abechi, additional, Kemi, Adedotun-Sulaiman, additional, Olubusuyi, Adewunmi M., additional, Oluwapelumi, Adeyemi O. O., additional, Hammami, Adnène, additional, Amuri, Adrienne A., additional, Sayed, Ahmad, additional, Ouma, Ahmed E. O., additional, Elargoubi, Aida, additional, Ajayi, Nnennaya A., additional, Victoria, Ajogbasile F., additional, Kazeem, Akano, additional, George, Akpede, additional, Trotter, Alexander J., additional, Yahaya, Ali A., additional, Keita, Alpha K., additional, Diallo, Amadou, additional, Kone, Amadou, additional, Souissi, Amal, additional, Chtourou, Amel, additional, Gutierrez, Ana V., additional, Page, Andrew J., additional, Vinze, Anika, additional, Iranzadeh, Arash, additional, Lambisia, Arnold, additional, Ismail, Arshad, additional, Rosemary, Audu, additional, Sylverken, Augustina, additional, Femi, Ayoade, additional, Ibrahimi, Azeddine, additional, Marycelin, Baba, additional, Oderinde, Bamidele S., additional, Bolajoko, Bankole, additional, Dhaala, Beatrice, additional, Herring, Belinda L., additional, Njanpop-Lafourcade, Berthe-Marie, additional, Kleinhans, Bronwyn, additional, McInnis, Bronwyn, additional, Tegomoh, Bryan, additional, Brook, Cara, additional, Pratt, Catherine B., additional, Scheepers, Cathrine, additional, Akoua-Koffi, Chantal G., additional, Agoti, Charles N., additional, Peyrefitte, Christophe, additional, Daubenberger, Claudia, additional, Morang’a, Collins M., additional, Nokes, D. James, additional, Amoako, Daniel G., additional, Bugembe, Daniel L., additional, Park, Danny, additional, Baker, David, additional, Doolabh, Deelan, additional, Ssemwanga, Deogratius, additional, Tshiabuila, Derek, additional, Bassirou, Diarra, additional, Amuzu, Dominic S. Y., additional, Goedhals, Dominique, additional, Omuoyo, Donwilliams O., additional, Maruapula, Dorcas, additional, Foster-Nyarko, Ebenezer, additional, Lusamaki, Eddy K., additional, Simulundu, Edgar, additional, Ong’era, Edidah M., additional, Ngabana, Edith N., additional, Shumba, Edwin, additional, El Fahime, Elmostafa, additional, Lokilo, Emmanuel, additional, Mukantwari, Enatha, additional, Philomena, Eromon, additional, Belarbi, Essia, additional, Simon-Loriere, Etienne, additional, Anoh, Etilé A., additional, Leendertz, Fabian, additional, Ajili, Faida, additional, Enoch, Fakayode O., additional, Wasfi, Fares, additional, Abdelmoula, Fatma, additional, Mosha, Fausta S., additional, Takawira, Faustinos T., additional, Derrar, Fawzi, additional, Bouzid, Feriel, additional, Onikepe, Folarin, additional, Adeola, Fowotade, additional, Muyembe, Francisca M., additional, Tanser, Frank, additional, Dratibi, Fred A., additional, Mbunsu, Gabriel K., additional, Thilliez, Gaetan, additional, Kay, Gemma L., additional, Githinji, George, additional, van Zyl, Gert, additional, Awandare, Gordon A., additional, Schubert, Grit, additional, Maphalala, Gugu P., additional, Ranaivoson, Hafaliana C., additional, Lemriss, Hajar, additional, Anise, Happi, additional, Abe, Haruka, additional, Karray, Hela H., additional, Nansumba, Hellen, additional, Elgahzaly, Hesham A., additional, Gumbo, Hlanai, additional, Smeti, Ibtihel, additional, Ayed, Ikhlas B., additional, Odia, Ikponmwosa, additional, Ben Boubaker, Ilhem Boutiba, additional, Gaaloul, Imed, additional, Gazy, Inbal, additional, Mudau, Innocent, additional, Ssewanyana, Isaac, additional, Konstantinus, Iyaloo, additional, Lekana-Douk, Jean B., additional, Makangara, Jean-Claude C., additional, Tamfum, Jean-Jacques M., additional, Heraud, Jean-Michel, additional, Shaffer, Jeffrey G., additional, Giandhari, Jennifer, additional, Li, Jingjing, additional, Yasuda, Jiro, additional, Mends, Joana Q., additional, Kiconco, Jocelyn, additional, Morobe, John M., additional, Gyapong, John O., additional, Okolie, Johnson C., additional, Kayiwa, John T., additional, Edwards, Johnathan A., additional, Gyamfi, Jones, additional, Farah, Jouali, additional, Nakaseegu, Joweria, additional, Ngoi, Joyce M., additional, Namulondo, Joyce, additional, Andeko, Julia C., additional, Lutwama, Julius J., additional, O’Grady, Justin, additional, Siddle, Katherine, additional, Adeyemi, Kayode T., additional, Tumedi, Kefentse A., additional, Said, Khadija M., additional, Hae-Young, Kim, additional, Duedu, Kwabena O., additional, Belyamani, Lahcen, additional, Fki-Berrajah, Lamia, additional, Singh, Lavanya, additional, Martins, Leonardo de O., additional, Tyers, Lynn, additional, Ramuth, Magalutcheemee, additional, Mastouri, Maha, additional, Aouni, Mahjoub, additional, el Hefnawi, Mahmoud, additional, Matsheka, Maitshwarelo I., additional, Kebabonye, Malebogo, additional, Diop, Mamadou, additional, Turki, Manel, additional, Paye, Marietou, additional, Nyaga, Martin M., additional, Mareka, Mathabo, additional, Damaris, Matoke-Muhia, additional, Mburu, Maureen W., additional, Mpina, Maximillian, additional, Nwando, Mba, additional, Owusu, Michael, additional, Wiley, Michael R., additional, Youtchou, Mirabeau T., additional, Ayekaba, Mitoha O., additional, Abouelhoda, Mohamed, additional, Seadawy, Mohamed G., additional, Khalifa, Mohamed K., additional, Sekhele, Mooko, additional, Ouadghiri, Mouna, additional, Diagne, Moussa M., additional, Mwenda, Mulenga, additional, Allam, Mushal, additional, Phan, My V. T., additional, Abid, Nabil, additional, Touil, Nadia, additional, Rujeni, Nadine, additional, Kharrat, Najla, additional, Ismael, Nalia, additional, Dia, Ndongo, additional, Mabunda, Nedio, additional, Hsiao, Nei-yuan, additional, Silochi, Nelson B., additional, Nsenga, Ngoy, additional, Gumede, Nicksy, additional, Mulder, Nicola, additional, Ndodo, Nnaemeka, additional, Razanajatovo, Norosoa H, additional, Iguosadolo, Nosamiefan, additional, Judith, Oguzie, additional, Kingsley, Ojide C., additional, Sylvanus, Okogbenin, additional, Peter, Okokhere, additional, Femi, Oladiji, additional, Idowu, Olawoye, additional, Testimony, Olumade, additional, Chukwuma, Omoruyi E., additional, Ogah, Onwe E., additional, Onwuamah, Chika K., additional, Cyril, Oshomah, additional, Faye, Ousmane, additional, Tomori, Oyewale, additional, Ondoa, Pascale, additional, Combe, Patrice, additional, Semanda, Patrick, additional, Oluniyi, Paul E., additional, Arnaldo, Paulo, additional, Quashie, Peter K., additional, Dussart, Philippe, additional, Bester, Phillip A., additional, Mbala, Placide K., additional, Ayivor-Djanie, Reuben, additional, Njouom, Richard, additional, Phillips, Richard O., additional, Gorman, Richmond, additional, Kingsley, Robert A., additional, Carr, Rosina A. A., additional, El Kabbaj, Saâd, additional, Gargouri, Saba, additional, Masmoudi, Saber, additional, Sankhe, Safietou, additional, Lawal, Salako B., additional, Kassim, Samar, additional, Trabelsi, Sameh, additional, Metha, Samar, additional, Kammoun, Sami, additional, Lemriss, Sanaâ, additional, Agwa, Sara H. A., additional, Calvignac-Spencer, Sébastien, additional, Schaffner, Stephen F., additional, Doumbia, Seydou, additional, Mandanda, Sheila M., additional, Aryeetey, Sherihane, additional, Ahmed, Shymaa S., additional, Elhamoumi, Siham, additional, Andriamandimby, Soafy, additional, Tope, Sobajo, additional, Lekana-Douki, Sonia, additional, Prosolek, Sophie, additional, Ouangraoua, Soumeya, additional, Mundeke, Steve A., additional, Rudder, Steven, additional, Panji, Sumir, additional, Pillay, Sureshnee, additional, Engelbrecht, Susan, additional, Nabadda, Susan, additional, Behillil, Sylvie, additional, Budiaki, Sylvie L., additional, van der Werf, Sylvie, additional, Mashe, Tapfumanei, additional, Aanniz, Tarik, additional, Mohale, Thabo, additional, Le-Viet, Thanh, additional, Schindler, Tobias, additional, Anyaneji, Ugochukwu J., additional, Chinedu, Ugwu, additional, Ramphal, Upasana, additional, Jessica, Uwanibe, additional, George, Uwem, additional, Fonseca, Vagner, additional, Enouf, Vincent, additional, Gorova, Vivianne, additional, Roshdy, Wael H., additional, Ampofo, William K., additional, Preiser, Wolfgang, additional, Choga, Wonderful T., additional, Bediako, Yaw, additional, Naidoo, Yeshnee, additional, Butera, Yvan, additional, de Laurent, Zaydah R., additional, Sall, Amadou A., additional, Rebai, Ahmed, additional, von Gottberg, Anne, additional, Kouriba, Bourema, additional, Williamson, Carolyn, additional, Bridges, Daniel J., additional, Chikwe, Ihekweazu, additional, Bhiman, Jinal N., additional, Mine, Madisa, additional, Cotten, Matthew, additional, Moyo, Sikhulile, additional, Gaseitsiwe, Simani, additional, Saasa, Ngonda, additional, Sabeti, Pardis C., additional, Kaleebu, Pontiano, additional, Tebeje, Yenew K., additional, Tessema, Sofonias K., additional, Happi, Christian, additional, Nkengasong, John, additional, and de Oliveira, Tulio, additional

Published
2021
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16. Additional file 1 of Performance evaluation of RDT, light microscopy, and PET-PCR for detecting Plasmodium falciparum malaria infections in the 2018 Zambia National Malaria Indicator Survey

Author

Mwenda, Mulenga C., Fola, Abebe A., Ciubotariu, Ilinca I., Mulube, Conceptor, Mambwe, Brenda, Kasaro, Rachael, Hawela, Moonga B., Hamainza, Busiku, Miller, John M., Carpi, Giovanna, and Bridges, Daniel J.

Abstract

Additional file 1: Table S1. Comparison of RDT and LM diagnostic metrics per province in Zambia.

Published
2021
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17. Performance Evaluation of RDT, Light Microscopy, and PET-PCR for Detecting P. Falciparum Malaria Infections in the 2018 Zambia National Malaria Indicator Survey

Author

Mwenda, Mulenga C., primary, Fola, Abebe A., additional, Ciubotariu, Ilinca I., additional, Mulube, Conceptor, additional, Mambwe, Brenda, additional, Kasaro, Rachael, additional, Hawela, Moonga B., additional, Hamainza, Busiku, additional, Miller, John M., additional, Carpi, Giovanna, additional, and Bridges, Daniel James, additional

Published
2021
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19. Detection of B.1.351 SARS-CoV-2 Variant Strain--Zambia, December 2020

Author

Mwenda, Mulenga, Saasa, Ngonda, Sinyange, Nyambe, Busby, George, Chipimo, Peter J., Hendry, Jason, Kapona, Otridah, Yingst, Samuel, Hines, Jonas Z., Minchella, Peter, Simulundu, Edgar, Changula, Katendi, Nalubamba, King Shimumbo, Sawa, Hirofumi, Kajihara, Masahiro, Yamagishi, Junya, Kapin'a, Muzala, Kapata, Nathan, Fwoloshi, Sombo, Zulu, Paul, Mulenga, Lloyd B., Agolory, Simon, Mukonka, Victor, and Bridges, Daniel J.

Subjects
Genomics -- Health aspects -- Reports, Coronaviruses -- Reports -- Health aspects, Health
Abstract

On February 17, 2021, this report was posted as an MMWR Early Release on the MMWR website (https://www.cdc.gov/mmwr). The first laboratory-confirmed cases of coronavirus disease 2019 (COVID-19), the illness caused [...]

Published
2021

20. Data on selected antimalarial drug resistance markers in Zambia

Author

Sitali, Lungowe, primary, Mwenda, Mulenga C., additional, Miller, John M., additional, Bridges, Daniel J., additional, Hawela, Moonga B., additional, Hamainza, Busiku, additional, Mudenda-Chilufya, Mutinta, additional, Chizema-Kawesha, Elizabeth, additional, Daniels, Rachel, additional, Eisele, Thomas P., additional, Nerland, Audun H., additional, Chipeta, James, additional, and Lindtjorn, Bernt, additional

Published
2021
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21. Surveillance of molecular markers for antimalarial resistance in Zambia: Polymorphism of Pfkelch 13, Pfmdr1 and Pfdhfr/Pfdhps genes

Author

Sitali, Lungowe, primary, Mwenda, Mulenga C., additional, Miller, John M., additional, Bridges, Daniel J., additional, Hawela, Moonga B., additional, Hamainza, Busiku, additional, Mudenda-Chilufya, Mutinta, additional, Chizema-Kawesha, Elizabeth, additional, Daniels, Rachel F., additional, Eisele, Thomas P., additional, Nerland, Audun H., additional, Chipeta, James, additional, and Lindtjorn, Bernt, additional

Published
2020
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22. Prevalence of Plasmodium falciparum and Non-falciparum Infections by Photo-Induced Electron Transfer–PCR in a Longitudinal Cohort of Individuals Enrolled in a Mass Drug Administration Trial in Southern Province, Zambia

Author

Chishimba, Sandra, primary, Mwenda, Mulenga, additional, Mambwe, Brenda, additional, Mulube, Conceptor, additional, Chalwe, Victor, additional, Moonga, Hawela, additional, Hamainza, Busiku, additional, Chizema-Kawesha, Elizabeth, additional, Steketee, Richard W., additional, Domingo, Gonzalo, additional, Fraser, Maya, additional, Kahn, Maria, additional, Pal, Sampa, additional, Silumbe, Kafula, additional, Conner, Ruben O., additional, Bennett, Adam, additional, Porter, Travis R., additional, Eisele, Thomas P., additional, Miller, John M., additional, and Bridges, Daniel J., additional

Published
2020
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23. A Longitudinal Cohort to Monitor Malaria Infection Incidence during Mass Drug Administration in Southern Province, Zambia

Author

Bennett, Adam, primary, Porter, Travis R., additional, Mwenda, Mulenga C., additional, Yukich, Joshua O., additional, Finn, Timothy P., additional, Lungu, Chris, additional, Silumbe, Kafula, additional, Mambwe, Brenda, additional, Chishimba, Sandra, additional, Mulube, Conceptor, additional, Bridges, Daniel J., additional, Hamainza, Busiku, additional, Slutsker, Laurence, additional, Steketee, Richard W., additional, Miller, John M., additional, and Eisele, Thomas P., additional

Published
2020
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24. Evidence for Reduced Malaria Parasite Population after Application of Population-Level Antimalarial Drug Strategies in Southern Province, Zambia

Author

Daniels, Rachel F., primary, Schaffner, Stephen F., additional, Bennett, Adam, additional, Porter, Travis R., additional, Yukich, Joshua O., additional, Mulube, Conceptor, additional, Mambwe, Brenda, additional, Mwenda, Mulenga C., additional, Chishimba, Sandra, additional, Bridges, Daniel J., additional, Moonga, Hawela, additional, Hamainza, Busiku, additional, Chizema Kawesha, Elizabeth, additional, Miller, John M., additional, Steketee, Richard W., additional, Wirth, Dyann F., additional, Eisele, Thomas P., additional, Hartl, Daniel L., additional, and Volkman, Sarah K., additional

Published
2020
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25. Pyrethroid and Carbamate Resistance in Anopheles funestus Giles along Lake Kariba in Southern Zambia

Author

Chanda, Javan, primary, Saili, Kochelani, additional, Phiri, Foustina, additional, Stevenson, Jennifer C., additional, Mwenda, Mulenga, additional, Chishimba, Sandra, additional, Mulube, Conceptor, additional, Mambwe, Brenda, additional, Lungu, Christopher, additional, Earle, Duncan, additional, Bennett, Adam, additional, Eisele, Thomas P., additional, Kamuliwo, Mulakwa, additional, Steketee, Richard W., additional, Keating, Joseph, additional, Miller, John M., additional, and Sikaala, Chadwick H., additional

Published
2020
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26. Incremental impact upon malaria transmission of supplementing pyrethroid-impregnated long-lasting insecticidal nets with indoor residual spraying using pyrethroids or the organophosphate, pirimiphos methyl

Author

Hamainza, Busiku, primary, Sikaala, Chadwick H., additional, Moonga, Hawela B., additional, Chanda, Javan, additional, Chinula, Dingani, additional, Mwenda, Mulenga, additional, Kamuliwo, Mulakwa, additional, Bennett, Adam, additional, Seyoum, Aklilu, additional, and Killeen, Gerry F., additional

Published
2016
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27. Therapeutic efficacy of artemether-lumefantrine on treatment of uncomplicated Plasmodium falciparum mono-infection in an area of high malaria transmission in Zambia

Author

Hamainza, Busiku, primary, Masaninga, Freddie, additional, Moonga, Hawela, additional, Mwenda, Mulenga, additional, Chanda-kapata, Pascalina, additional, Chalwe, Victor, additional, Chanda, Emmanuel, additional, Kamuliwo, Mulakwa, additional, and Babaniyi, Olusegun Ayorinde, additional

Published
2014
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29. A cost-effective, community-based, mosquitotrapping scheme that captures spatial and temporal heterogeneities of malaria transmission in rural Zambia.

Author

Sikaala, Chadwick H., Chinula, Dingani, Chanda, Javan, Hamainza, Busiku, Mwenda, Mulenga, Mukali, Isabel, Kamuliwo, Mulakwa, Lobo, Neil F., Seyoum, Aklilu, and Killeen, Gerry F.

Subjects
MOSQUITO vectors, MALARIA, MOSQUITO control, DISEASE management, POPULATION dynamics
Abstract

Background Monitoring mosquito population dynamics is essential to guide selection and evaluation of malaria vector control interventions but is typically implemented by mobile, centrallymanaged teams who can only visit a limited number of locations frequently enough to capture longitudinal trends. Community-based (CB) mosquito trapping schemes for parallel, continuous monitoring of multiple locations are therefore required that are practical, affordable, effective, and reliable. Methods A CB surveillance scheme with a monthly sampling and reporting cycle for capturing malaria vectors, using Centers for Disease Control and Prevention light traps (LT) and Ifakara Tent Traps (ITT), were conducted by trained community health workers (CHW) in 14 clusters of households immediately surrounding health facilities in rural south-east Zambia. At the end of the study, a controlled quality assurance (QA) survey was conducted by a centrally supervised expert team using human landing catch (HLC), LT and ITT to evaluate accuracy of the CB trapping data. Active surveillance of malaria parasite infection rates amongst humans was conducted by CHWs in the same clusters to determine the epidemiological relevance of these CB entomological surveys. Results CB-LT and CB-ITT exhibited relative sampling efficiencies of 39 and 9%, respectively, compared with QA surveys using the same traps. However, cost per sampling night was lowest for CB-LT ($13.6), followed closely by CB-ITT ($18.0), both of which were far less expensive than any QA survey (HLC: $138, LT: $289, ITT: $269). Cost per specimen of Anopheles funestus captured was lowest for CB-LT ($5.3), followed by potentially hazardous QA-HLC ($10.5) and then CB-ITT ($28.0), all of which were far more cost-effective than QA-LT ($141) and QA-ITT ($168). Time-trends of malaria diagnostic positivity (DP) followed those of An. funestus density with a one-month lag and the wide range of mean DP across clusters was closely associated with mean densities of An. funestus caught by CB-LT (P < 0.001). Conclusions CB trapping schemes appear to be far more affordable, epidemiologically relevant and costeffective than centrally supervised trapping schemes and may well be applicable to enhance intervention trials and even enable routine programmatic monitoring of vector population dynamics on unprecedented national scales. [ABSTRACT FROM AUTHOR]

Published
2014
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30. National genomic profiling of Plasmodium falciparum antimalarial resistance in Zambian children participating in the 2018 Malaria Indicator Survey.

Author

Fola AA, Ciubotariu II, Dorman J, Mwenda MC, Mambwe B, Mulube C, Kasaro R, Hawela MB, Hamainza B, Miller JM, Bailey JA, Moss WJ, Bridges DJ, and Carpi G

Abstract

The emergence of antimalarial drug resistance is a major threat to malaria control and elimination. Using whole genome sequencing of 282 P. falciparum samples collected during the 2018 Zambia National Malaria Indicator Survey, we determined the prevalence and spatial distribution of known and candidate antimalarial drug resistance mutations. High levels of genotypic resistance were found across Zambia to pyrimethamine, with over 94% (n=266) of samples having the Pfdhfr triple mutant (N51 I , C59 R , and S108 N ), and sulfadoxine, with over 84% (n=238) having the Pfdhps double mutant (A437 G and K540 E ). In northern Zambia, 5.3% (n=15) of samples also harbored the Pfdhps A581 G mutation. Although 29 mutations were identified in Pfkelch13 , these mutations were present at low frequency (<2.5%), and only three were WHO-validated artemisinin partial resistance mutations: P441 L (n=1, 0.35%), V568 M (n=2, 0.7%) and R622 T (n=1, 0.35%). Notably, 91 (32%) of samples carried the E431 K mutation in the Pfatpase6 gene, which is associated with artemisinin resistance. No specimens carried any known mutations associated with chloroquine resistance in the Pfcrt gene (codons 72-76). P. falciparum strains circulating in Zambia were highly resistant to sulfadoxine and pyrimethamine but remained susceptible to chloroquine and artemisinin. Despite this encouraging finding, early genetic signs of developing artemisinin resistance highlight the urgent need for continued vigilance and expanded routine genomic surveillance to monitor these changes.

Published
2024
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31. Entomological effects of attractive targeted sugar bait station deployment in Western Zambia: vector surveillance findings from a two-arm cluster randomized phase III trial.

Author

Wagman J, Chanda B, Chanda J, Saili K, Orange E, Mambo P, Muyabe R, Kaniki T, Mwenya M, Ng'andu M, Sakala J, Ngulube W, Miller J, Arnzen A, Silumbe K, Mwaanga G, Simubali L, Mungo A, Mburu MM, Simulundu E, Mambwe B, Kasaro R, Mulube C, Mwenda M, Hamainza B, Ashton RA, Eisele TP, Harris AF, Entwistle J, Yukich J, Slutsker L, Burkot TR, and Littrell M

Subjects
Zambia, Animals, Female, Humans, Sugars, Malaria prevention & control, Anopheles physiology, Mosquito Vectors physiology, Mosquito Control methods
Abstract

Background: Attractive targeted sugar bait (ATSB) stations are a novel tool with potential to complement current approaches to malaria vector control. To assess the public health value of ATSB station deployment in areas of high coverage with standard vector control, a two-arm cluster-randomized controlled trial (cRCT) of Sarabi ATSB® stations (Westham Ltd., Hod-Hasharon, Israel) was conducted in Western Province, Zambia, a high-burden location were Anopheles funestus is the dominant vector. The trial included 70 clusters and was designed to measure the effect of ATSBs on case incidence and infection prevalence over two 7-month deployments. Reported here are results of the vector surveillance component of the study, conducted in a subset of 20 clusters and designed to provide entomological context to guide overall interpretation of trial findings., Methods: Each month, 200 paired indoor-outdoor human landing catch (HLC) and 200 paired light trap (LT) collections were conducted to monitor An. funestus parity, abundance, biting rates, sporozoite prevalence, and entomological inoculation rates (EIR)., Results: During the study 20,337 female An. funestus were collected, 11,229 from control and 9,108 from intervention clusters. A subset of 3,131 HLC specimens were assessed for parity: The mean non-parous proportion was 23.0% (95% CI 18.2-28.7%, total n = 1477) in the control and 21.2% (95% CI 18.8-23.9%, total n = 1654) in the intervention arm, an OR = 1.05 (95% CI 0.82-1.34; p = 0.688). A non-significant reduction in LT abundance (RR = 0.65 [95% CI 0.30-1.40, p = 0.267]) was associated with ATSB deployment. HLC rates were highly variable, but model results indicate a similar non-significant trend with a RR = 0.68 (95%CI 0.22-2.00; p = 0.479). There were no effects on sporozoite prevalence or EIR., Conclusions: Anopheles funestus parity did not differ across study arms, but ATSB deployment was associated with a non-significant 35% reduction in vector LT density, results that are consistent with the epidemiological impact reported elsewhere. Additional research is needed to better understand how to maximize the potential impact of ATSB approaches in Zambia and other contexts., Trial Registration Number: This trial was registered with Clinicaltrials.gov (NCT04800055, 16 March 2021)., (© 2024. The Author(s).)

Published
2024
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32. Diversity and selection analyses identify transmission-blocking antigens as the optimal vaccine candidates in Plasmodium falciparum .

Author

Ciubotariu II, Broyles BK, Xie S, Thimmapuram J, Mwenda MC, Mambwe B, Mulube C, Matoba J, Schue JL, Moss WJ, Bridges DJ, He Q, and Carpi G

Abstract

Background: A highly effective vaccine for malaria remains an elusive target, at least in part due to the under-appreciated natural parasite variation. This study aimed to investigate genetic and structural variation, and immune selection of leading malaria vaccine candidates across the Plasmodium falciparum 's life cycle., Methods: We analyzed 325 P. falciparum whole genome sequences from Zambia, in addition to 791 genomes from five other African countries available in the MalariaGEN Pf3k Rdatabase. Ten vaccine antigens spanning three life-history stages were examined for genetic and structural variations, using population genetics measures, haplotype network analysis, and 3D structure selection analysis., Findings: Among the ten antigens analyzed, only three in the transmission-blocking vaccine category display P . falciparum 3D7 as the dominant haplotype. The antigens AMA1, CSP, MSP1 19 and CelTOS, are much more diverse than the other antigens, and their epitope regions are under moderate to strong balancing selection. In contrast, Rh5 , a blood stage antigen, displays low diversity yet slightly stronger immune selection in the merozoite-blocking epitope region. Except for CelTOS , the transmission-blocking antigens Pfs25 , Pfs48/45 , Pfs230 , Pfs47 , and Pfs28 exhibit minimal diversity and no immune selection in epitopes that induce strain-transcending antibodies, suggesting potential effectiveness of 3D7-based vaccines in blocking transmission., Interpretations: These findings offer valuable insights into the selection of optimal vaccine candidates against P. falciparum . Based on our results, we recommend prioritizing conserved merozoite antigens and transmission-blocking antigens. Combining these antigens in multi-stage approaches may be particularly promising for malaria vaccine development initiatives., Funding: Purdue Department of Biological Sciences; Puskas Memorial Fellowship; National Institute of Allergy and Infectious Diseases (U19AI089680)., Competing Interests: Declaration of interests All authors declare no competing interests.

Published
2024
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33. Genomics reveals heterogeneous Plasmodium falciparum transmission and population differentiation in Zambia and bordering countries.

Author

Fola AA, He Q, Xie S, Thimmapuram J, Bhide KP, Dorman J, Ciubotariu II, Mwenda MC, Mambwe B, Mulube C, Hawela M, Norris DE, Moss WJ, Bridges DJ, and Carpi G

Abstract

Genomic surveillance plays a critical role in monitoring malaria transmission and understanding how the parasite adapts in response to interventions. We conducted genomic surveillance of malaria by sequencing 241 Plasmodium falciparum genomes from regions with varying levels of malaria transmission across Zambia. We found genomic evidence of high levels of within-host polygenomic infections, regardless of epidemiological characteristics, underscoring the extensive and ongoing endemic malaria transmission in the country. We identified country-level clustering of parasites from Zambia and neighboring countries, and distinct clustering of parasites from West Africa. Within Zambia, our identity by descent (IBD) relatedness analysis uncovered spatial clustering of closely related parasite pairs at the local level and rare cases of long-distance sharing. Genomic regions with large shared IBD segments and strong positive selection signatures identified genes involved in sulfadoxine-pyrimethamine and artemisinin combination therapies drug resistance, but no signature related to chloroquine resistance. Together, our findings enhance our understanding of P. falciparum transmission nationwide in Zambia and highlight the urgency of strengthening malaria control programs and surveillance of antimalarial drug resistance.

Published
2024
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34. The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance.

Author

Tegally H, San JE, Cotten M, Moir M, Tegomoh B, Mboowa G, Martin DP, Baxter C, Lambisia AW, Diallo A, Amoako DG, Diagne MM, Sisay A, Zekri AN, Gueye AS, Sangare AK, Ouedraogo AS, Sow A, Musa AO, Sesay AK, Abias AG, Elzagheid AI, Lagare A, Kemi AS, Abar AE, Johnson AA, Fowotade A, Oluwapelumi AO, Amuri AA, Juru A, Kandeil A, Mostafa A, Rebai A, Sayed A, Kazeem A, Balde A, Christoffels A, Trotter AJ, Campbell A, Keita AK, Kone A, Bouzid A, Souissi A, Agweyu A, Naguib A, Gutierrez AV, Nkeshimana A, Page AJ, Yadouleton A, Vinze A, Happi AN, Chouikha A, Iranzadeh A, Maharaj A, Batchi-Bouyou AL, Ismail A, Sylverken AA, Goba A, Femi A, Sijuwola AE, Marycelin B, Salako BL, Oderinde BS, Bolajoko B, Diarra B, Herring BL, Tsofa B, Lekana-Douki B, Mvula B, Njanpop-Lafourcade BM, Marondera BT, Khaireh BA, Kouriba B, Adu B, Pool B, McInnis B, Brook C, Williamson C, Nduwimana C, Anscombe C, Pratt CB, Scheepers C, Akoua-Koffi CG, Agoti CN, Mapanguy CM, Loucoubar C, Onwuamah CK, Ihekweazu C, Malaka CN, Peyrefitte C, Grace C, Omoruyi CE, Rafaï CD, Morang'a CM, Erameh C, Lule DB, Bridges DJ, Mukadi-Bamuleka D, Park D, Rasmussen DA, Baker D, Nokes DJ, Ssemwanga D, Tshiabuila D, Amuzu DSY, Goedhals D, Grant DS, Omuoyo DO, Maruapula D, Wanjohi DW, Foster-Nyarko E, Lusamaki EK, Simulundu E, Ong'era EM, Ngabana EN, Abworo EO, Otieno E, Shumba E, Barasa E, Ahmed EB, Ahmed EA, Lokilo E, Mukantwari E, Philomena E, Belarbi E, Simon-Loriere E, Anoh EA, Manuel E, Leendertz F, Taweh FM, Wasfi F, Abdelmoula F, Takawira FT, Derrar F, Ajogbasile FV, Treurnicht F, Onikepe F, Ntoumi F, Muyembe FM, Ragomzingba FEZ, Dratibi FA, Iyanu FA, Mbunsu GK, Thilliez G, Kay GL, Akpede GO, van Zyl GU, Awandare GA, Kpeli GS, Schubert G, Maphalala GP, Ranaivoson HC, Omunakwe HE, Onywera H, Abe H, Karray H, Nansumba H, Triki H, Kadjo HAA, Elgahzaly H, Gumbo H, Mathieu H, Kavunga-Membo H, Smeti I, Olawoye IB, Adetifa IMO, Odia I, Ben Boubaker IB, Muhammad IA, Ssewanyana I, Wurie I, Konstantinus IS, Halatoko JWA, Ayei J, Sonoo J, Makangara JC, Tamfum JM, Heraud JM, Shaffer JG, Giandhari J, Musyoki J, Nkurunziza J, Uwanibe JN, Bhiman JN, Yasuda J, Morais J, Kiconco J, Sandi JD, Huddleston J, Odoom JK, Morobe JM, Gyapong JO, Kayiwa JT, Okolie JC, Xavier JS, Gyamfi J, Wamala JF, Bonney JHK, Nyandwi J, Everatt J, Nakaseegu J, Ngoi JM, Namulondo J, Oguzie JU, Andeko JC, Lutwama JJ, Mogga JJH, O'Grady J, Siddle KJ, Victoir K, Adeyemi KT, Tumedi KA, Carvalho KS, Mohammed KS, Dellagi K, Musonda KG, Duedu KO, Fki-Berrajah L, Singh L, Kepler LM, Biscornet L, de Oliveira Martins L, Chabuka L, Olubayo L, Ojok LD, Deng LL, Ochola-Oyier LI, Tyers L, Mine M, Ramuth M, Mastouri M, ElHefnawi M, Mbanne M, Matsheka MI, Kebabonye M, Diop M, Momoh M, Lima Mendonça MDL, Venter M, Paye MF, Faye M, Nyaga MM, Mareka M, Damaris MM, Mburu MW, Mpina MG, Owusu M, Wiley MR, Tatfeng MY, Ayekaba MO, Abouelhoda M, Beloufa MA, Seadawy MG, Khalifa MK, Matobo MM, Kane M, Salou M, Mbulawa MB, Mwenda M, Allam M, Phan MVT, Abid N, Rujeni N, Abuzaid N, Ismael N, Elguindy N, Top NM, Dia N, Mabunda N, Hsiao NY, Silochi NB, Francisco NM, Saasa N, Bbosa N, Murunga N, Gumede N, Wolter N, Sitharam N, Ndodo N, Ajayi NA, Tordo N, Mbhele N, Razanajatovo NH, Iguosadolo N, Mba N, Kingsley OC, Sylvanus O, Femi O, Adewumi OM, Testimony O, Ogunsanya OA, Fakayode O, Ogah OE, Oludayo OE, Faye O, Smith-Lawrence P, Ondoa P, Combe P, Nabisubi P, Semanda P, Oluniyi PE, Arnaldo P, Quashie PK, Okokhere PO, Bejon P, Dussart P, Bester PA, Mbala PK, Kaleebu P, Abechi P, El-Shesheny R, Joseph R, Aziz RK, Essomba RG, Ayivor-Djanie R, Njouom R, Phillips RO, Gorman R, Kingsley RA, Neto Rodrigues RMDESA, Audu RA, Carr RAA, Gargouri S, Masmoudi S, Bootsma S, Sankhe S, Mohamed SI, Femi S, Mhalla S, Hosch S, Kassim SK, Metha S, Trabelsi S, Agwa SH, Mwangi SW, Doumbia S, Makiala-Mandanda S, Aryeetey S, Ahmed SS, Ahmed SM, Elhamoumi S, Moyo S, Lutucuta S, Gaseitsiwe S, Jalloh S, Andriamandimby SF, Oguntope S, Grayo S, Lekana-Douki S, Prosolek S, Ouangraoua S, van Wyk S, Schaffner SF, Kanyerezi S, Ahuka-Mundeke S, Rudder S, Pillay S, Nabadda S, Behillil S, Budiaki SL, van der Werf S, Mashe T, Mohale T, Le-Viet T, Velavan TP, Schindler T, Maponga TG, Bedford T, Anyaneji UJ, Chinedu U, Ramphal U, George UE, Enouf V, Nene V, Gorova V, Roshdy WH, Karim WA, Ampofo WK, Preiser W, Choga WT, Ahmed YA, Ramphal Y, Bediako Y, Naidoo Y, Butera Y, de Laurent ZR, Ouma AEO, von Gottberg A, Githinji G, Moeti M, Tomori O, Sabeti PC, Sall AA, Oyola SO, Tebeje YK, Tessema SK, de Oliveira T, Happi C, Lessells R, Nkengasong J, and Wilkinson E

Subjects
Africa epidemiology, Genomics, Humans, COVID-19 epidemiology, COVID-19 virology, Epidemiological Monitoring, Pandemics, SARS-CoV-2 genetics
Abstract

Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing in Africa over the past year has led to a major increase in the number of sequences that have been generated and used to track the pandemic on the continent, a number that now exceeds 100,000 genomes. Our results show an increase in the number of African countries that are able to sequence domestically and highlight that local sequencing enables faster turnaround times and more-regular routine surveillance. Despite limitations of low testing proportions, findings from this genomic surveillance study underscore the heterogeneous nature of the pandemic and illuminate the distinct dispersal dynamics of variants of concern-particularly Alpha, Beta, Delta, and Omicron-on the continent. Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve while the continent faces many emerging and reemerging infectious disease threats. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century.

Published
2022
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35. Detection of B.1.351 SARS-CoV-2 Variant Strain - Zambia, December 2020.

Author

Mwenda M, Saasa N, Sinyange N, Busby G, Chipimo PJ, Hendry J, Kapona O, Yingst S, Hines JZ, Minchella P, Simulundu E, Changula K, Nalubamba KS, Sawa H, Kajihara M, Yamagishi J, Kapin'a M, Kapata N, Fwoloshi S, Zulu P, Mulenga LB, Agolory S, Mukonka V, and Bridges DJ

Subjects
Adult, COVID-19 epidemiology, COVID-19 Nucleic Acid Testing, Female, Humans, Male, Middle Aged, SARS-CoV-2 isolation & purification, Zambia epidemiology, COVID-19 diagnosis, COVID-19 virology, SARS-CoV-2 genetics
Abstract

The first laboratory-confirmed cases of coronavirus disease 2019 (COVID-19), the illness caused by SARS-CoV-2, in Zambia were detected in March 2020 (1). Beginning in July, the number of confirmed cases began to increase rapidly, first peaking during July-August, and then declining in September and October (Figure). After 3 months of relatively low case counts, COVID-19 cases began rapidly rising throughout the country in mid-December. On December 18, 2020, South Africa published the genome of a SARS-CoV-2 variant strain with several mutations that affect the spike protein (2). The variant included a mutation (N501Y) associated with increased transmissibility. † , § SARS-CoV-2 lineages with this mutation have rapidly expanded geographically. , ** The variant strain (PANGO [Phylogenetic Assignment of Named Global Outbreak] lineage B.1.351 †† ) was first detected in the Eastern Cape Province of South Africa from specimens collected in early August, spread within South Africa, and appears to have displaced the majority of other SARS-CoV-2 lineages circulating in that country (2). As of January 10, 2021, eight countries had reported cases with the B.1.351 variant. In Zambia, the average number of daily confirmed COVID-19 cases increased 16-fold, from 44 cases during December 1-10 to 700 during January 1-10, after detection of the B.1.351 variant in specimens collected during December 16-23. Zambia is a southern African country that shares substantial commerce and tourism linkages with South Africa, which might have contributed to the transmission of the B.1.351 variant between the two countries., Competing Interests: All authors have completed and submitted the International Committee of Medical Journal Editors form for disclosure of potential conflicts of interest. Edgar Simulundu reports receipt of reagents from Hokkaido University during the course of the study. No other potential conflicts of interest were disclosed.

Published
2021
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36. Data on selected antimalarial drug resistance markers in Zambia.

Author

Sitali L, Mwenda MC, Miller JM, Bridges DJ, Hawela MB, Hamainza B, Mudenda-Chilufya M, Chizema-Kawesha E, Daniels R, Eisele TP, Nerland AH, Chipeta J, and Lindtjorn B

Abstract

This article describes data on selected resistance markers for antimalarial drugs used in Zambia. Antimalarial drug resistance has hindered the progress in the control and elimination of malaria. Blood samples were collected during a cross-sectional household survey, conducted during the peak malaria transmission, April to May of 2017. Dried blood spots were collected during the survey and transported to a laboratory for analysis. The analysed included polymerase chain reaction (PCR) followed by high resolution melt (HRM) for mutations associated with Sulfadoxine-pyrimethamine resistance in the Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and P. falciparum dihydropteroate synthase (Pfdhps) genes. Mutations associated with artemether-lumefantrine resistance in falciparum multi-drug resistance gene 1 ( Pfmdr1 ) were also assessed using PCR and HRM analysis, whereas the P. falciparum Kelch 13 ( PfK13 ) gene was assessed using nested PCR followed by amplicon sequencing., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article., (© 2020 The Authors.)

Published
2020
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