Search

Abstract Despite the advances in paleogenomics, red cell blood group systems in ancient human populations remain scarcely known. Pioneer attempts showed that Neandertal and Denisova, two archaic hominid populations inhabiting Eurasia, expressed blood groups currently found in sub-Saharans and a rare “rhesus”, part of which is found in Oceanians. Herein we fully pictured the blood group genetic diversity of 22 Homo sapiens and 14 Neandertals from Eurasia living between 120,000 and 20,000 years before present (yBP). From the ABO, Rh, Kell, Duffy, Kidd, MNS, Diego, H, secretor and Indian systems, we noted that the blood group allele diversity in the Neandertals remained unchanged since 120,000 yBP, while H. sapiens conquered Eurasia with blood group alleles presently exclusive to non-African populations, suggesting they may have differentiated right after the Out of Africa, between 70,000 and 45,000 yBP. Notably, Ust’Ishim possessed unknown alleles that may illustrate the lost genetic heritage of the early Eurasians. Lastly, Neandertals shared a unique Rh haplotype from which we updated the current RHD phylogeny. The contribution of this study is twofold. It enlightens the expansion patterns of H sapiens and recalls the anthropological effectiveness of genetic polymorphisms currently being surveyed for transfusion safety and pregnancy monitoring.

In this study we describe an adult left first pollical (thumb) proximal phalanx (I2-104) from the Baume de Moula-Guercy (Ardèche, France) and we evaluate its taxonomic status. We first describe this bone in detail, taking into account its pathology, before comparing it, through multivariate analyses, with a diverse sample of recent and fossil humans. Based on metric and morphological comparisons, we show that this phalanx belongs to a Neandertal individual who suffered from osteoarthritis. Osteoarthritis can have different origins, including overuse, degeneration, trauma and infection. Each of these possible etiologies is explored in our study. Although the cause is difficult to identify, the pathology in this individual may represent an inflammatory reaction caused by repeated and intense vibrations provoked by the high-frequency knapping of the left hand over time. [ABSTRACT FROM AUTHOR]

Copyright of Cuaternario y Geomorfología is the property of Cuaternario y Geomorfologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Background The genomes of present-day non-Africans are composed of 1–3% of Neandertal-derived DNA as a consequence of admixture events between Neandertals and anatomically modern humans about 50–60 thousand years ago. Neandertal-introgressed single nucleotide polymorphisms (aSNPs) have been associated with modern human disease-related traits, which are risk factors for pancreatic ductal adenocarcinoma (PDAC), such as obesity, type 2 diabetes, and inflammation. In this study, we aimed at investigating the role of aSNPs in PDAC in three Eurasian populations. Results The high-coverage Vindija Neandertal genome was used to select aSNPs in non-African populations from 1000 Genomes project phase 3 data. Then, the association between aSNPs and PDAC risk was tested independently in Europeans and East Asians, using existing GWAS data on more than 200 000 individuals. We did not find any significant associations between aSNPs and PDAC in samples of European descent, whereas, in East Asians, we observed that the Chr10p12.1-rs117585753-T allele (MAF = 10%) increased the risk to develop PDAC (OR = 1.35, 95%CI 1.19–1.54, P = 3.59 × 10–6), with a P-value close to a threshold that takes into account multiple testing. Conclusions Our results show only a minimal contribution of Neandertal SNPs to PDAC risk.

Background: The genomes of present-day non-Africans are composed of 1–3% of Neandertal-derived DNA as a consequence of admixture events between Neandertals and anatomically modern humans about 50–60 thousand years ago. Neandertal-introgressed single nucleotide polymorphisms (aSNPs) have been associated with modern human disease-related traits, which are risk factors for pancreatic ductal adenocarcinoma (PDAC), such as obesity, type 2 diabetes, and inflammation. In this study, we aimed at investigating the role of aSNPs in PDAC in three Eurasian populations. Results: The high-coverage Vindija Neandertal genome was used to select aSNPs in non-African populations from 1000 Genomes project phase 3 data. Then, the association between aSNPs and PDAC risk was tested independently in Europeans and East Asians, using existing GWAS data on more than 200 000 individuals. We did not find any significant associations between aSNPs and PDAC in samples of European descent, whereas, in East Asians, we observed that the Chr10p12.1-rs117585753-T allele (MAF = 10%) increased the risk to develop PDAC (OR = 1.35, 95%CI 1.19–1.54, P = 3.59 × 10–6), with a P-value close to a threshold that takes into account multiple testing. Conclusions: Our results show only a minimal contribution of Neandertal SNPs to PDAC risk. [ABSTRACT FROM AUTHOR]

The admixture between modern humans and Neandertals has resulted in ∼2% of the genomes of present-day non-Africans being composed of Neandertal DNA. Introgressed Neandertal DNA has been demonstrated to significantly affect the transcriptomic landscape in people today and via this molecular mechanism influence phenotype variation as well. However, little is known about how much of that regulatory impact is mediated through long-range regulatory effects that have been shown to explain ∼20% of expression variation. Here we identified 60 transcription factors (TFs) with their top cis-eQTL SNP in GTEx being of Neandertal ancestry and predicted long-range Neandertal DNA-induced regulatory effects by screening for the predicted target genes of those TFs. We show that the TFs form a significantly connected protein–protein interaction network. Among them are JUN and PRDM5, two brain-expressed TFs that have their predicted target genes enriched in regions devoid of Neandertal DNA. Archaic cis-eQTLs for the 60 TFs include multiple candidates for local adaptation, some of which show significant allele frequency increases over the last ∼10,000 years. A large proportion of the cis-eQTL-associated archaic SNPs have additional associations with various immune traits, schizophrenia, blood cell type composition and anthropometric measures. Finally, we demonstrate that our results are consistent with those of Neandertal DNA-associated empirical trans-eQTLs. Our results suggest that Neandertal DNA significantly influences regulatory networks, that its regulatory reach goes beyond the 40% of genomic sequence it still covers in present-day non-Africans and that via the investigated mechanism Neandertal DNA influences the phenotypic variation in people today. [ABSTRACT FROM AUTHOR]

One of the features that distinguishes modern humans from our extinct relatives and ancestors is a globular shape of the braincase [1-4]. As the endocranium closely mirrors the outer shape of the brain, these differences might reflect altered neural architecture [4, 5]. However, in the absence of fossil brain tissue, the underlying neuroanatomical changes as well as their genetic bases remain elusive. To better understand the biological foundations of modern human endocranial shape, we turn to our closest extinct relatives: the Neandertals. Interbreeding between modern humans and Neandertals has resulted in introgressed fragments of Neandertal DNA in the genomes of present-day non-Africans [6, 7]. Based on shape analyses of fossil skull endocasts, we derive a measure of endocranial globularity from structural MRI scans of thousands of modern humans and study the effects of introgressed fragments of Neandertal DNA on this phenotype. We find that Neandertal alleles on chromosomes 1 and 18 are associated with reduced endocranial globularity. These alleles influence expression of two nearby genes, UBR4 and PHLPP1, which are involved in neurogenesis and myelination, respectively. Our findings show how integration of fossil skull data with archaic genomics and neuroimaging can suggest developmental mechanisms that may contribute to the unique modern human endocranial shape.

Svante Pääbo, a founding director of the Max-Planck Institute for Evolutionary Anthropology in Leipzig, Germany, was awarded the 2022 Nobel Prize in Physiology or Medicine for his contribution to the development of the field of paleogenomics. His work on sequencing early hominin DNA has led to advancements in extraction and analysis of ancient DNA. The advancement of paleogenomics can further our understanding of modern humans, their ancestors, and human evolution as well as potentially predicting emerging and re-emerging diseases. A bibliometric analysis of Dr. Pääbo's work follows a general overview of ancient DNA analysis. [ABSTRACT FROM AUTHOR]

The Southern chamois (Rupicapra pyrenaica) is a small-sized bovid that currently inhabits the Cantabrian Mountain Range, the Pyrenees, and the Central Apennine Mountains. This species was exploited as a resource by Palaeolithic human groups of the northern region of the Iberian Peninsula, standing out in the record of the Cave of Valdegoba. The fossil record of this site has provided plenty of evidence of Neanderthal activity. According to taphonomic analyses, Neanderthals had primary access to prey and chamois was the most consumed species. Analysis of Valdegoba’s Southern chamois allows consideration of: (1), the age structure and the dynamics of the population; (2) the palaeobiological characteristics of this population (e.g. mortality rate by age intervals, growth rate or body mass); (3) comparison of the population dynamics of Valdegoba’s chamois with that of present-day populations of different species (R. rupicapra, R. pyrenaica); and (4) exploitation of the chamois by Neanderthals. We focussed on methodological aspects of population structure and mortality profiles using life tables with vital statistics, Leslie-Lewis matrices and ternary diagrams starting from tooth eruption and wear, whereas mass estimates were obtained from the postcranial bones. Cohort structures from extant Southern, Alpine and New Zealand chamois populations were compared to Valdegoba’s chamois to better understand the paleoenvironmental context of this fossil species. In addition, we calculated the amount of meat available to the Neanderthals and the extent of their range and its population implications. Our results show that the age structure of Valdegoba chamois reflects a very similar structure and ecological features to present-day populations. Moreover, modelling the age structure of Valdegoba population shows that Neanderthals could have exploited the Southern chamois without leading to the collapse of the population. Based on the results of energetic inputs related to the percentage of chamois in Valdegoba, where we estimate that a Neanderthal group would have had to exploit at least an area of between 61 and 99 km2 to cover their energetic requirements. [ABSTRACT FROM AUTHOR]

Taurodontism is a continuous anatomical variation of permanent and primary posterior teeth represented by an enlargement of the pulp cavity. A high prevalence of the trait is reported in Homo neanderthalensis remains. Exploring and refining epidemiology of taurodontism in actual populations could strengthen the hypothesis of a selective advantage for a high attrition diet (as heavy tooth wear in Homo sapiens evolution changed little until recently) or favour pleiotropic or genetic drift effects to explain the high frequency of the trait in Neandertal remains. Prevalence ranges between 0.1% and 48% in the literature. The aim of the present study is to assess the prevalence of taurodontism in recent populations by means of meta-analysis, that is, is the prevalence of taurodontism lower or higher in modern human living populations, where the selective advantages of high attrition diet are still expected? From 90 potentially eligible studies, 15 were included in the meta-analysis. Only cross-sectional studies were reported, and 14,771 participants were included. The meta-analyses were performed with a random model, calculating a weighted-mean prevalence of 11.8%. Gender was found to be unrelated to the prevalence of taurodontism (OR = 0.84 (95% CI 0.67–1.05), p > 0.05). Taurodontism occurs in approximately 11.8% of the living population. This result questions the status of taurodontism as a “typical trait” in Homo neanderthalensis and allows a possible common evolutionary mechanism in Homo sapiens and Homo neanderthalensis for the trait. Further studies should include more accurate and standardized methods to assess the condition.

En el transcurso de una intervención arqueológica en el solar nº 6 de la calle Artekale 6 de la villa de Plentzia (Bizkaia) se localizó un conjunto lítico sobre el sustrato rocoso y bajo un depósito de arcillas de 40 cm de espesor. En este trabajo se describe este conjunto de núcleos y lascas, y se propone una atribución del conjunto al Paleolítico Medio, probablemente reciente. Además, se discute el papel de estos breves asentamientos en las estrategias de asentamiento de los Neandertales en esta región. ABSTRACT: In the course of an archaeological intervention in the site of Artekale Street 6 in the town of Plentzia (Bizkaia), a lithic assemblage was found on the bedrock below 40 cm of clayey sediment. This paper describes the assemblage of cores and flakes, and proposes an attribution to the Middle Palaeolithic, probably recent. In addition, the role of these short-term occupations in regional Neanderthal settlement strategies is discussed.

IntroductionReconstructing the dietary and behavioral strategies of our hominin ancestors is crucial to understanding their evolution, adaptation, and overall way of life. Teeth in general, and dental microwear specifically, provide a means to examine these strategies, with posterior teeth well positioned to tell us about diet, and anterior teeth helping us examine non-dietary tooth-use behaviors. Past research predominantly focused on strategies of adult individuals, leaving us to wonder the role children may have played in the community at large. Here we begin to address this by analyzing prehistoric and historic children through dental microwear texture analysis of deciduous anterior teeth.Materials and MethodsFour sample groups were used: Neandertals (N = 8), early modern humans (N = 14), historic Egyptians from Amarna (N = 19) and historic high-Arctic Inuit from Point Hope, Alaska (N = 6). Anterior deciduous teeth were carefully cleaned, molded, and cast with high-resolution materials. Labial surfaces were scanned for dental microwear textures using two white-light confocal microscopes at the University of Arkansas, and a soft filter applied to facilitate data comparisons.Results and DiscussionResults show that dental microwear texture analysis successfully differentiated the samples by all texture variables examined (anisotropy, complexity, scale of maximum complexity, and two variants of heterogeneity). Interestingly, the Neandertal and Point Hope children had similar mean values across all the texture variables, and both groups were significantly different from the Amarna, Egyptian children. These differences suggest diversity in abrasive load exposure and participation in non-dietary anterior tooth-use behaviors. Further analyses and an expanded sample size will help to strengthen the data presented here, but our results show that some prehistoric and historic children took part in similar behaviors as their adult counterparts.

Although some variation introgressed from Neanderthals has undergone selective sweeps, little is known about its functional significance. We used a Massively Parallel Reporter Assay (MPRA) to assay 5,353 high-frequency introgressed variants for their ability to modulate the gene expression within 170 bp of endogenous sequence. We identified 2,548 variants in active putative cis -regulatory elements (CREs) and 292 expression-modulating variants (emVars). These emVars are predicted to alter the binding motifs of important immune transcription factors, are enriched for associations with neutrophil and white blood cell count, and are associated with the expression of genes that function in innate immune pathways including inflammatory response and antiviral defense. We combined the MPRA data with other data sets to identify strong candidates to be driver variants of positive selection including an emVar that may contribute to protection against severe COVID-19 response. We endogenously deleted two CREs containing expression-modulation variants linked to immune function, rs11624425 and rs80317430, identifying their primary genic targets as ELMSAN1 , and PAN2 and STAT2 , respectively, three genes differentially expressed during influenza infection. Overall, we present the first database of experimentally identified expression-modulating Neanderthal-introgressed alleles contributing to potential immune response in modern humans. [ABSTRACT FROM AUTHOR]

Neandertal DNA makes up 2–3% of the genomes of all non-African individuals. The patterns of Neandertal ancestry in modern humans have been used to estimate that this is the result of gene flow that occurred during the expansion of modern humans into Eurasia, but the precise dates of this event remain largely unknown. Here, we introduce an extended admixture pulse model that allows joint estimation of the timing and duration of gene flow. This model leads to simple expressions for both the admixture segment distribution and the decay curve of ancestry linkage disequilibrium, and we show that these two statistics are closely related. In simulations, we find that estimates of the mean time of admixture are largely robust to details in gene flow models, but that the duration of the gene flow can only be recovered if gene flow is very recent and the exact recombination map is known. These results imply that gene flow from Neandertals into modern humans could have happened over hundreds of generations. Ancient genomes from the time around the admixture event are thus likely required to resolve the question when, where, and for how long humans and Neandertals interacted. [ABSTRACT FROM AUTHOR]

Em ‘Neandertal, Nosso Irmão – Uma Breve História do Homem’, Silvana Condemi e François Savatier conseguem um feito quase único: sumarizar, de forma completa, instigante e fluida, tudo o que sabemos sobre nossa espécie-irmã, como o livro defende. Resultado de inúmeras horas de pesquisa e encontros em cafés, conforme relata Savatier, o livro pode facilmente ser considerado como um ideal de divulgação científica, ao transformar uma massa grande e confusa de informações em uma narrativa atrativa, linear e criativa. Mas atenção: a linearidade aqui, que se refere à escolha das sequências de temas, é bastante positiva e de forma alguma limita o caráter informativo do texto.

In 1945, the activity of the quarries settled near the village of Montmaurin, 75km south-west from Toulouse in France, led to the discovery of several caves filled by archeological deposits. After the visit done by H. Begouën and the Abbey H. Breuil, L. Méroc did excavations from 1946 to 1961 mainly in the Coupe-Gorge cavity which has yielded a lot of lithics and bones. Among them, there were human remains: a juvenile partial mandible (corresponding to the symphyseal part), a right maxillary bone bearing P4-C and 3 isolated teeth (1 canine, P3 and M3). In a nearby vertical gallery called La Niche, one complete adult mandible bearing its molars, 2 vertebras and one fragmentary tibia were also discovered. All these fossils, except the 3 latter ones, where published in detail. But, due to the lack of radiometric dating, such fossils were less and less included in studies. The purpose of this paper is to re-examine these human remains which are relevant to discuss the emergence of the Neandertal lineage in Europe. Indeed, the mandible from La Niche, dated to the IS7 based on biochronology, is not fully Neandertal but it shows a combination of archaic and derived features, respectively on the bone itself and on teeth, which keeps open the discussion.

Copyright of Complutum is the property of Universidad Complutense de Madrid and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The inference of Neanderthal cognition, including their cultural and linguistic capabilities, has persisted as a fiercely debated research topic for decades. This lack of consensus is substantially based on inherent uncertainties in reconstructing prehistory out of indirect evidence as well as other methodological limitations. Further factors include systemic difficulties within interdisciplinary discourse, data artifacts, historic research biases, and the sheer scope of the relevant research. Given the degrees of freedom in interpretation ensuing from these complications, any attempt to find approximate answers to the yet unsettled pertinent discourse may not rest on single studies, but instead a careful and comprehensive interdisciplinary synthesis of findings. Triangulating Neanderthals' cognition by considering the plethora of data, diverse perspectives and aforementioned complexities present within the literature constitutes the currently most reliable pathway to tentative conclusions. While some uncertainties remain, such an approach paints the picture of an extensive shared humanity between anatomically modern humans and Neanderthals. This article is categorized under:Cognitive Biology > Evolutionary Roots of CognitionLinguistics > Evolution of Language [ABSTRACT FROM AUTHOR]

Differential diagnosis and tabulation of cases of dental agenesis in Middle and Upper Paleolithic Western Eurasian humans to synthesize this data and to test previous hypotheses about when recent human patterns of third molar agenesis were established. 139 Late Pleistocene human remains and 149 individuals from three Epi-Paleolithic/ Holocene non-agricultural comparative collections. All remains were visually and radiographically recorded by the author. In addition to establishing that third molar agenesis was common during the Late Upper Paleolithic (22,500–10,000 years BP), this study suggests a pattern of increasing prevalence through time. An increase in the prevalence of third molar agenesis in the Late Upper Paleolithic could indicate selection for dental size reduction and orthognathy, but also bio-cultural changes from more intensive food preparation techniques. Third molar agenesis, a well-known developmental defect, is often reported for recent human skeletal collections, but the prevalence of the condition for Pleistocene hominins had not been previously quantified in order to consider patterns through time. Hypotheses posited for the high prevalence of third molar agenesis, or hypodontia in general, in some recent human groups require an understanding of the prevalence of these traits in the past. Paleolithic skeletal remains are incomplete, so these values are under-estimations. Individuals are also separated diachronically and geographically and should not be assumed to represent a single population sample. Hypotheses on some of the potential selective forces acting on dental size reduction and subsequent agenesis could be tested in recent humans. [ABSTRACT FROM AUTHOR]

The recovery of additional mandibular fossils from the Atapuerca Sima de los Huesos (SH) site provides new insights into the evolutionary significance of this sample. In particular, morphological descriptions of the new adult specimens are provided, along with standardized metric data and phylogenetically relevant morphological features for the expanded adult sample. The new and more complete specimens extend the known range of variation in the Atapuerca (SH) mandibles in some metric and morphological details. In other aspects, the addition of new specimens has made it possible to confirm previous observations based on more limited evidence. Pairwise comparisons of individual metric variables revealed the only significant difference between the Atapuerca (SH) hominins and Neandertals was a more vertical symphysis in the latter. Similarly, principal components analysis of size-adjusted variables showed a strong similarity between the Atapuerca (SH) hominins and Neandertals. Morphologically, the Atapuerca (SH) mandibles show nearly the full complement of Neandertal-derived features. Nevertheless, the Neandertals differ from the Atapuerca (SH) mandibles in showing a high frequency of the H/O mandibular foramen, a truncated, thinned and inverted gonial margin, a high placement of the mylohyoid line at the level of the M3, a more vertical symphysis and somewhat more pronounced expression of the chin structures. Size-related morphological variation in the SH hominins includes larger retromolar spaces, more posterior placement of the lateral corpus structures, and stronger markings associated with the muscles of mastication in larger specimens. However, phylogenetically relevant features in the SH sample are fairly stable and do not vary with the overall size of the mandible. Direct comparison of the enlarged mandibular sample from Atapuerca (SH) with the Mauer mandible, the type specimen of H. heidelbergensis, reveals important differences from the SH hominins, and there is no morphological counterpart of Mauer within the SH sample, suggesting the SH fossils should not be assigned to this taxon. The Atapuerca (SH) mandibles show a greater number of derived Neandertal features, particularly those related to midfacial prognathism and in the configuration of the superior ramus, than other European middle Pleistocene specimens. This suggests that more than one evolutionary lineage co-existed in the middle Pleistocene, and, broadly speaking, it appears possible to separate the European middle Pleistocene mandibular remains into two distinct groupings. One group shows a suite of derived Neandertal features and includes specimens from the sites of Atapuerca (SH), Payre, l'Aubesier and Ehringsdorf. The other group includes specimens that generally lack derived Neandertal features and includes the mandibles from the sites of Mauer, Mala Balanica, Montmaurin and (probably) Visogliano. The two published Arago mandibles differ strongly from one another, with Arago 2 probably belonging to this former group, and Neandertal affinities being more difficult to identify in Arago 13. Outside of the SH sample, derived Neandertal features in the mandible only become more common during the second half of the middle Pleistocene. Acceptance of a cladogenetic pattern of evolution during the European middle Pleistocene has the potential to reconcile the predictions of the accretion model and the two phases model for the appearance of Neandertal morphology. The precise taxonomic classification of the SH hominins must contemplate features from the dentition, cranium, mandible and postcranial skeleton, all of which are preserved at the SH site. Nevertheless, the origin of the Neandertal clade may be tied to a speciation event reflected in the appearance of a suite of derived Neandertal features in the face, dentition and mandible, all of which are present in the Atapuerca (SH) hominins. This same suite of features also provides a useful anatomical basis to include other European middle Pleistocene mandibles and crania within the Neandertal clade., (© 2023 The Authors. The Anatomical Record published by Wiley Periodicals LLC on behalf of American Association for Anatomy.)

For a long time, the rich bone industries of the Upper Palaeolithic were opposed to the opportunistic Neandertal bone tools among which the bone retoucher was the most common type. The recent finding of a few shaped bone tools into Mousterian contexts has been taken as an emergence of a "modern behaviour". However, this outlook is based on biased corpuses. On one side, the large number of unshaped bone tools recently discovered in Upper Palaeolithic assemblages leads us to reconsider what a bone industry can be. On the other side, the increasing discoveries of bone tools in more ancient contexts indicates that this type of production is not strictly linked to Homo sapiens. Chagyrskaya cave, located in the Siberian Altai, brings us the opportunity to discuss this question. Dated around 50,000 years BP, the site yielded a local facies of Mousterian lithic industry associated to several Neandertal remains. A technological and functional analysis of the faunal remains reveal more than one thousand bone tools. Most are retouchers, but a significant part belongs to other morpho-functional categories: intermediate tools, retouched tools and tools with a smoothed end. Even though these tools were mainly manufactured by direct percussion, their number and the recurrence of their morphological and traceological features lead us to consider them as a true bone industry. Far from the Homo sapiens standards, this industry has its own coherence that needs now to be understood. [ABSTRACT FROM AUTHOR]

Objectives: We provide the description and comparative analysis of all the human fossil remains found at Axlor during the excavations carried out by J. M. de Barandiarán from 1967 to 1974: a cranial vault fragment and seven teeth, five of which likely belonged to the same individual, although two are currently lost. Our goal is to describe in detail all these human remains and discuss both their taxonomic attribution and their stratigraphic context. Materials and methods: We describe external and internal anatomy, and use classic and geometric morphometrics. The teeth from Axlor are compared to Neandertals, Upper Paleolithic, and recent modern humans. Results: Two teeth (a left dm2, a left di1) and the parietal fragment show morphological features consistent with a Neandertal classification, and were found in an undisturbed Mousterian context. The remaining three teeth (plus the two lost ones), initially classified as Neandertals, show morphological features and a general size that are more compatible with their classification as modern humans. Discussion: A left parietal fragment (Level VIII) from a single probably adult Neandertal individual was recovered during the old excavations performed by Barandiarán. Additionally, two different Neandertal children lost deciduous teeth during the formations of levels V (left di1) and IV (right dm2). In addition, a modern human individual is represented by five remains (two currently lost) from a complex stratigraphic setting. Some of the morphological features of these remains suggest that they may represent one of the scarce examples of Upper Paleolithic modern human remains in the northern Iberian Peninsula, which should be confirmed by direct dating. [ABSTRACT FROM AUTHOR]

Anatomically modern humans carry many introgressed variants from other hominins in their genomes. Some of them affect their phenotype and can thus be negatively or positively selected. Several individual genes have been proposed to be the subject of adaptive introgression, but the possibility of polygenic adaptive introgression has not been extensively investigated yet. In this study, we analyze archaic introgression maps with refined functional enrichment methods to find signals of polygenic adaptation of introgressed variants. We first apply a method to detect sets of connected genes (subnetworks) within biological pathways that present higher-than-expected levels of archaic introgression. We then introduce and apply a new statistical test to distinguish between epistatic and independent selection in gene sets of present-day humans. We identify several known targets of adaptive introgression, and we show that they belong to larger networks of introgressed genes. After correction for genetic linkage, we find that signals of polygenic adaptation are mostly explained by independent and potentially sequential selection episodes. However, we also find some gene sets where introgressed variants present significant signals of epistatic selection. Our results confirm that archaic introgression has facilitated local adaptation, especially in immunity related and metabolic functions and highlight its involvement in a coordinated response to pathogens out of Africa. [ABSTRACT FROM AUTHOR]

Sex is a biological trait fundamental to the study of hominin fossils. Among the many questions that can be addressed are those related to taxonomy, biological variability, sexual dimorphism, paleoobstetrics, funerary selection, and paleodemography. While new methodologies such as paleogenomics or paleoproteomics can be used to determine sex, they have not been systematically applied to Pleistocene human remains due to their destructive nature. Therefore, we estimated sex from the coxal bone of the newly discovered pelvic remains of the Regourdou 1 Neandertal (Southwest France, MIS 5) based on morphological and metric data employing two methods that have been recently revised and shown to be reliable in multiple studies. Both methods calculate posterior probabilities of the estimate. The right coxal bone of Regourdou 1 was partially reconstructed providing additional traits for sex estimation. These methods were cross validated on 14 sufficiently preserved coxal bones of specimens from the Neandertal lineage. Our results show that the Regourdou 1 individual, whose postcranial skeleton is not robust, is a male, and that previous sex attributions of comparative Neandertal specimens are largely in agreement with those obtained here. Our results encourage additional morphological research of fossil hominins in order to develop a set of methods that are applicable, reliable, and reproducible. [ABSTRACT FROM AUTHOR]

Abstract Background Small insertions and deletions occur in humans at a lower rate compared to nucleotide changes, but evolve under more constraint than nucleotide changes. While the evolution of insertions and deletions have been investigated using ape outgroups, the now available genome of a Neandertal can shed light on the evolution of indels in more recent times. Results We used the Neandertal genome together with several primate outgroup genomes to differentiate between human insertion/deletion changes that likely occurred before the split from Neandertals and those that likely arose later. Changes that pre-date the split from Neandertals show a smaller proportion of deletions than those that occurred later. The presence of a Neandertal-shared allele in Europeans or Asians but the absence in Africans was used to detect putatively introgressed indels in Europeans and Asians. A larger proportion of these variants reside in intergenic regions compared to other modern human variants, and some variants are linked to SNPs that have been associated with traits in modern humans. Conclusions Our results are in agreement with earlier results that suggested that deletions evolve under more constraint than insertions. When considering Neandertal introgressed variants, we find some evidence that negative selection affected these variants more than other variants segregating in modern humans. Among introgressed variants we also identify indels that may influence the phenotype of their carriers. In particular an introgressed deletion associated with a decrease in the time to menarche may constitute an example of a former Neandertal-specific trait contributing to modern human phenotypic diversity.

Los denticulados y las muescas son dos de los elementos más frecuentes en la panoplia musteriense. El creciente interés por el estudio de estos tipos permite vislumbrar su complejidad. La aparente estandarización de sus formas no lo es tal a la luz de nuevos estudios tipométricos. Así mismo, la especialización supuesta del denticulado parece encontrarse ahora en entredicho a partir de los nuevos datos revelados por estudios traceológicos que apuntan a que se trata de una pieza en esencia versátil. Presentamos en este artículo los resultados de un estudio funcional realizado sobre una muestra de denticulados y muescas en cuarzo procedente del yacimiento musteriense del Abrigo de Navalmaíllo (Pinilla del Valle, Madrid, España). Las huellas de uso sobre elementos de cuarzo son peculiares dadas las especiales características de la materia prima. Por otro lado, normalmente se conservan mejor que las formadas en materiales de mejor calidad como el sílex. Los resultados del estudio corroboran la versatilidad de dichas piezas para funciones distintas relacionadas, entre otras, con alguna de las actividades implicadas en el trabajo complejo de descuartizado y el trabajo de la madera.

Spy I from the Meuse River Basin of Belgium is among the most recent Neandertals. This adult lived at the terminus of Marine Isotope Stage (MIS) 3 in cold steppe environments at the northern edge of the habitable zone for Neandertals where plants were relatively scarce. The dietary proclivities of Spy I are reconstructed using dental microwear texture analysis and compared to 33 Neandertals from western Eurasia, MIS 5 to MIS 3. Spy I has an elevated enamel surface complexity suggesting the consumption of course dietary items such as wild seeds, acorns, nuts, and underground storage organs laden with particles of grit. Unlike the young and old individuals from Hortus with low values for anisotropy, Spy I is closest to the adults from this site suggesting a common pattern of masticatory behavior typified this life cycle stage. Like many other Neandertals, Spy I probably consumed plant foods at appreciable levels, some of which were hard and brittle or poorly processed. [ABSTRACT FROM AUTHOR]

The long-standing debate around the origin and evolutionary mode of the Neandertal lineage is connected to the understanding of the large morphological diversity found in the European Middle Pleistocene Hominin (EMPH) samples. In practical terms, this problem can be approached through two questions: How many morphs can be distinguished in EMPH and how do they relate to the origin of Neandertals? These questions are addressed in this paper by means of geometric morphometric comparative analyses in combination with principles of craniofacial biology. We use the mandible as a model system because of its relative abundance in the fossil record, the presence of diagnostic features relevant to the process under analysis, and the key role played by some specimens. Among them, three samples are central in the debate: the Mauer mandible (as the holotype of Homo heidelbergensis), and the Arago and Atapuerca-SH samples. Our comparative analyses conclude that the Mauer and Arago samples derived from a single deme, and that Neandertal derived features that represent an incipient phase of the evolutionary process. The specimen AT-950 from the Atapuerca-SH sample has a number of structural similarities with Mauer, Arago and Montmaurin, which allows linking of this latter group with the complete Atapuerca-SH hypodigm. Consequently, all of these specimens are considered members of the Neandertal lineage. The fossil record illustrates a long evolutionary Neandertal lineage going back at least 0.6 million years. Against populational-based interpretations, we favor a structuralist approach and follow Enlow's counterpart growth analysis and compensatory growth mechanisms for interpretation of the EMPH diversity. In this framework, morphometric cranio-mandibular variation in hominin samples from the European Middle Pleistonece must be contemplated within the head form vertical variation pattern (e.g. vertical dolicho- and brachyfacial form spectrum) widespread in Hominoid species. • A morphogenetic structuralist perspective is provided. • A two species model is supported for a long evolutionary Neandertal lineage. • European Middle Pleistocene hominin samples are classified as Homo heidelbergensis. • Arago II, Mauer and AT-950 mandibles share a form pattern. • A plesiomorphic facial variation pattern explains European Middle Pleistocene forms. [ABSTRACT FROM AUTHOR]

Objectives: We provide the description and comparative analysis of six new teeth from the site of La Ferrassie. Our goal is to discuss their taxonomic attribution, and to provide an updated inventory of Neandertal and modern human remains from La Ferrassie in their associated archeological context. Materials and methods: We use external and internal anatomy, classic morphometrics, and geometric morphometrics. The teeth from La Ferrassie are compared to several samples of contemporary Neandertals and upper Paleolithic modern humans and to recent modern humans. Results: Three specimens are classified as Neandertals, two as modern humans, and one remains unclassified. Discussion: Based on the previously known fossil samples and the new teeth reported here, there are currently a minimum of four adult and five immature Neandertal individuals coming from the "Grand Abri" and a minimum of two modern human adult individuals: one from "Grand Abri" and one from "Grotte." It is noteworthy that the spatial distribution of the recovered Neandertal remains is not restricted to the area where the LF1–LF 8 were found but now covers the full extension of the excavated area. Moreover, while both Neandertal and modern human occupations have yielded isolated human remains, the partial‐to‐complete skeletons only belong to Neandertals. These considerations open new perspectives for the understanding of the occupation and use of the La Ferrassie site. [ABSTRACT FROM AUTHOR]