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A major DSM-5 criterion for Alcohol Use Disorder is the continuation of alcohol consumption despite negative consequences. This has been modeled in mice using adulteration of alcohol solution with the bitter tastant quinine. Mice that continue to consume alcohol despite this adulteration are considered aversion-resistant. There have been a limited number of studies dissecting the underlying neuronal mechanisms of aversion-resistant drinking behaviors, and those only used male subjects. We have previously shown that female mice are more resistant to quinine adulteration of alcohol than males. Our aim is to identify potential sex differences in neuronal activation that may underlie this behavior.Male and female C57BL/6J mice were allowed continuous access to 20% alcohol in a two-bottle choice procedure. To test aversion-resistance, the alcohol was adulterated with increasing concentrations (0.03mM, 0.1mM, and 0.2mM) of quinine hydrocholoride. After consumption rates were calculated, brains were extracted to examine neuronal activation using Fos immunohistochemistry.We found that female mice suppressed their intake to a lesser extent than males when alcohol solution was adulterated with quinine. Our Fos staining revealed three regions of interest that exhibit a sex difference during quinine-adulterated alcohol drinking: the ventromedial prefrontal cortex (vmPFC), the posterior insular cortex (PIC), and the ventral tegmental area (VTA). Both the vmPFC and the PIC exhibited higher neuronal activation in males during quinine-adulterated alcohol consumption. However, females showed higher Fos activation in the VTA during quinine-adulterated alcohol consumption.Females more readily exhibit aversion-resistant alcohol intake than their male counterparts and exhibit some differences in neuronal activation patterns. These results indicate sex differences in neurocircuitry that may underlie compulsive drinking behaviors.

GrailQuest(Gamma Ray Astronomy International Laboratory for QUantum Exploration of Space-Time) is a mission concept based on a constellation (hundreds/thousands) of nano/micro/small-satellites in low (or near) Earth orbits. Each satellite hosts a non-collimated array of scintillator crystals coupled with Silicon Drift Detectors with broad energy band coverage (keV-MeV range) and excellent temporal resolution (≤ 100 nanoseconds) each with effective area$\sim 100 \text {cm}^{2}$∼100cm2. This simple and robust design allows for mass-production of the satellites of the fleet. This revolutionary approach implies a huge reduction of costs, flexibility in the segmented launching strategy, and an incremental long-term plan to increase the number of detectors and their performance; this will result in a living observatory for next-generation, space-based astronomical facilities.GrailQuestis conceived as an all-sky monitor for fast localisation of high signal-to-noise ratio transients in the X-/gamma-ray band, e.g. the elusive electromagnetic counterparts of gravitational wave events. Robust temporal triangulation techniques will allow unprecedented localisation capabilities, in the keV-MeV band, of a few arcseconds or below, depending on the temporal structure of the transient event. The ambitious ultimate goal of this mission is to perform the first experiment, in quantum gravity, to directly probe space-time structure down to the minuscule Planck scale, by constraining or measuring a first-order dispersion relation for lightin vacuo. This is obtained by detecting delays between photons of different energies in the prompt emission of Gamma-Ray Bursts.

Experimental observations of enzymes under active turnover conditions have brought new insight into the role of protein motions and allosteric networks in catalysis. Many of these studies characterize enzymes under dynamic chemical equilibrium conditions, in which the enzyme is actively catalyzing both the forward and reverse reactions during data acquisition. We have previously analyzed conformational dynamics and allosteric networks of the alpha subunit of tryptophan synthase under such conditions using NMR. We have proposed that this working state represents a four to one ratio of the enzyme bound with the indole-3-glycerol phosphate substrate (E:IGP) to the enzyme bound with the products indole and glyceraldehyde-3-phosphate (E:indole:G3P). Here, we analyze the inactive D60N variant to deconvolute the contributions of the substrate- and products-bound states to the working state. While the D60N substitution itself induces small structural and dynamic changes, the D60N E:IGP and E:indole:G3P states cannot entirely account for the conformational dynamics and allosteric networks present in the working state. The act of chemical bond breakage and/or formation, or possibly the generation of an intermediate, may alter the structure and dynamics present in the working state. As the enzyme transitions from the substrate-bound to the products-bound state, millisecond conformational exchange processes are quenched and new allosteric connections are made between the alpha active site and the surface which interfaces with the beta subunit. The structural ordering of the enzyme and these new allosteric connections may be important in coordinating the channeling of the indole product into the beta subunit.

A general framework by which dynamic interactions within a protein will promote the necessary series of structural changes, or "conformational cycle," required for function is proposed. It is suggested that the free-energy landscape of a protein is biased toward this conformational cycle. Fluctuations into higher energy, although thermally accessible, conformations drive the conformational cycle forward. The amino acid interaction network is defined as those intraprotein interactions that contribute most to the free-energy landscape. Some network connections are consistent in every structural state, while others periodically change their interaction strength according to the conformational cycle. It is reviewed here that structural transitions change these periodic network connections, which then predisposes the protein toward the next set of network changes, and hence the next structural change. These concepts are illustrated by recent work on tryptophan synthase. Disruption of these dynamic connections may lead to aberrant protein function and disease states.

Enzymes undergo a range of internal motions from local, active site fluctuations to large-scale, global conformational changes. These motions are often important for enzyme function, including in ligand binding and dissociation and even preparing the active site for chemical catalysis. Protein engineering efforts have been directed towards manipulating enzyme structural dynamics and conformational changes, including targeting specific amino acid interactions and creation of chimeric enzymes with new regulatory functions. Post-translational covalent modification can provide an additional level of enzyme control. These studies have not only provided insights into the functional role of protein motions, but they offer opportunities to create stimulus-responsive enzymes. These enzymes can be engineered to respond to a number of external stimuli, including light, pH, and the presence of novel allosteric modulators. Altogether, the ability to engineer and control enzyme structural dynamics can provide new tools for biotechnology and medicine.

This paper is the second in a series where we report the results of the long-term timing of the millisecond pulsars (MSPs) in 47 Tucanae with the Parkes 64-m radio telescope. We obtain improved timing parameters that provide additional information for studies of the cluster dynamics: a) the pulsar proper motions yield an estimate of the proper motion of the cluster as a whole ($\mu_{\alpha}\, = \, 5.00\, \pm \, 0.14\, \rm mas \, yr^{-1}$, $\mu_{\delta}\, = \, -2.84\, \pm \, 0.12\, \rm mas \, yr^{-1}$) and the motion of the pulsars relative to each other. b) We measure the second spin-period derivatives caused by the change of the pulsar line-of-sight accelerations; 47 Tuc H, U and possibly J are being affected by nearby objects. c) For ten binary systems we now measure changes in the orbital period caused by their acceleration in the gravitational field of the cluster. From all these measurements, we derive a cluster distance no smaller than $\sim\,$4.69 kpc and show that the characteristics of these MSPs are very similar to their counterparts in the Galactic disk. We find no evidence in favour of an intermediate mass black hole at the centre of the cluster. Finally, we describe the orbital behaviour of the four "black widow" systems. Two of them, 47 Tuc J and O, exhibit orbital variability similar to that observed in other such systems, while for 47 Tuc I and R the orbits seem to be remarkably stable. It appears, therefore, that not all "black widows" have unpredictable orbital behaviour., Comment: 21 pages in journal format, 9 figures, 4 tables, accepted for publication in MNRAS, several clarifications made and typos fixed

Our ability to engineer protein structure and function has grown dramatically over recent years. Perhaps the next level in protein design is to develop proteins whose function can be regulated in response to various stimuli, including ligand binding, pH changes, and light. Endeavors toward these goals have tested and expanded on our understanding of protein function and allosteric regulation. In this chapter, we provide examples from different methods for developing new allosterically regulated proteins. These methods range from whole insertion of regulatory domains into new host proteins, to covalent attachment of photoswitches to generate light-responsive proteins, and to targeted changes to specific amino acid residues, especially to residues identified to be important for relaying allosteric information across the protein framework. Many of the examples we discuss have already found practical use in medical and biotechnology applications.

Context: XB 1916-053 is a low mass X-ray binary system (LMXB) hosting a neutron star (NS) and showing periodic dips. The spectrum of the persistent emission was modeled with a blackbody component having a temperature between 1.31 and 1.67 keV and with a Comptonization component with an electron temperature of 9.4 keV and a photon index $\Gamma$ between 2.5 and 2.9. The presence of absorption features associated with highly ionized elements suggested the presence of partially ionized plasma in the system. Aims: In this work we performed a study of the spectrum of XB 1916-053, which aims to shed light on the nature of the seed photons that contribute to the Comptonization component. Methods: We analyzed three Suzaku observations of XB 1916-053: the first was performed in November 2006 and the others were carried out in October 2014. We extracted the persistent spectra from each observation and combined the spectra of the most recent observations, obtaining a single spectrum with a higher statistic. We also extracted and combined the spectra of the dips observed during the same observations. Results: On the basis of the available data statistics, we infer that the scenario in which the corona Comptonizes photons emitted both by the innermost region of the accretion disk and the NS surface is not statistically relevant with respect to the case in which only photons emitted by the NS surface are Comptonized. We find that the source is in a soft spectral state in all the analyzed observations. We detect the K$\alpha$ absorption lines of \ion{Fe}{xxv} and \ion{Fe}{xxvi}, which have already been reported in literature, and for the first time the K$\beta$ absorption lines of the same ions. We also detect an edge at 0.876 keV, which is consistent with a \ion{O}{viii} K absorption edge. (Abridged), Comment: 12 pages, 4 figures, accepted for publication in A&A

Tryptophan synthase is a model system for understanding allosteric regulation within enzyme complexes. Amino acid interaction networks were previously delineated in the isolated alpha subunit (αTS) in the absence of the beta subunit (βTS). The amino acid interaction networks were different between the ligand-free enzyme and the enzyme actively catalyzing turnover. Previous X-ray crystallography studies indicated only minor localized changes when ligands bind αTS, and so, structural changes alone could not explain the changes to the amino acid interaction networks. We hypothesized that the network changes could instead be related to changes in conformational dynamics. As such, we conducted nuclear magnetic resonance relaxation studies on different substrate- and products-bound complexes of αTS. Specifically, we collected 15N R2 relaxation dispersion data that reports on microsecond-to-millisecond timescale motion of backbone amide groups. These experiments indicated that there are conformational exchange events throughout αTS. Substrate and product binding change specific motional pathways throughout the enzyme, and these pathways connect the previously identified network residues. These pathways reach the αTS/βTS binding interface, suggesting that the identified dynamic networks may also be important for communication with the βTS subunit.

We report the discovery of X-ray pulsations at 105.2 Hz (9.5 ms) from the transient X-ray binary IGR J16597-3704 using NuSTAR and Swift. The source was discovered by INTEGRAL in the globular cluster NGC 6256 at a distance of 9.1 kpc. The X-ray pulsations show a clear Doppler modulation implying an orbital period of ~46 minutes and a projected semi-major axis of ~5 lt-ms, which makes IGR J16597-3704 an ultra-compact X-ray binary system. We estimated a minimum companion mass of 0.0065 solar masses, assuming a neutron star mass of 1.4 solar masses, and an inclination angle of, 4 pages, 4 images, Accepted for publication in A&A

We report on the detection of X-ray pulsations at 2.1 ms from the known X-ray burster IGR J17379-3747 using XMM-Newton. The coherent signal shows a clear Doppler modulation from which we estimate an orbital period of ~1.9 hours and a projected semi-major axis of ~8 lt-ms. Taking into account the lack of eclipses (inclination angle of < 75 deg) and assuming a neutron star mass of 1.4 Msun, we estimated a minimum companion star of ~0.06 Msun. Considerations on the probability distribution of the binary inclination angle make less likely the hypothesis of a main-sequence companion star. On the other hand, the close correspondence with the orbital parameters of the accreting millisecond pulsar SAX J1808.4-3658 suggests the presence of a bloated brown dwarf. The energy spectrum of the source is well described by a soft disk black-body component (kT ~0.45 keV) plus a Comptonisation spectrum with photon index ~1.9. No sign of emission lines or reflection components is significantly detected. Finally, combining the source ephemerides estimated from the observed outbursts, we obtained a first constraint on the long-term orbital evolution of the order of dP_orb/dt = (-2.5 +/- 2.3)E-12 s/s., 5 pages, 2 figures, 1 table. Accepted for publication in A&A

The Sardinia Radio Telescope (SRT) is a 64-m, fully-steerable single-dish radio telescope that was recently commissioned both technically and scientifically with regard to the basic observing modes. In order to improve the scientific capability and cover all the requirements for an advanced single-dish radio telescope, we developed the SArdinia Roach2-based Digital Architecture for Radio Astronomy (SARDARA), a wide-band, multi-feed, general-purpose, and reconfigurable digital platform, whose preliminary setup was used in the early science program of the SRT in 2016. In this paper, we describe the backend both in terms of its scientific motivation and technical design, how it has been interfaced with the telescope environment during its development and, finally, its scientific commissioning in different observing modes with single-feed receivers.

Networks of noncovalent interactions play important roles in the structural dynamics of globular proteins. Allosteric signals can propagate from the surface of an enzyme into its active site through these amino acid interaction networks. We used nuclear magnetic resonance chemical shift covariance analyses on a catalytically inactive variant of the alpha subunit of tryptophan synthase to map its amino acid interaction networks across its entire catalytic cycle. Each enzyme intermediate has unique sets of residues that display high chemical shift correlations across a set of alanine-to-glycine loop substitutions. Moreover, these chemical shift correlations systematically increase or decrease as a function of the catalytic cycle. These studies indicate that the enzyme intermediates have different amino acid interaction networks, and these network transitions are likely important for catalytic cycle progression. The different network interactions likely help to position catalytic machinery and coordinate function with the beta subunit.

Despite considerations of mass loss from stellar evolution suggesting otherwise, the content of gas in globular clusters seems poor and hence its measurement very elusive. One way of constraining the presence of ionized gas in a globular cluster is through its dispersive effects on the radiation of the millisecond pulsars included in the cluster. This effect led Freire et al. in 2001 to the first detection of any kind of gas in a globular cluster in the case of 47 Tucanae. By exploiting the results of 12 additional years of timing, as well as the observation of new millisecond pulsars in 47 Tucanae, we revisited this measurement: we first used the entire set of available timing parameters in order to measure the dynamical properties of the cluster and the three-dimensional position of the pulsars. Then we applied and tested various gas distribution models: assuming a constant gas density, we confirmed the detection of ionized gas with a number density of $n= 0.23\pm 0.05$ cm$^{-3}$, larger than the previous determination (at 2$\sigma$ uncertainty). Models predicting a decreasing density or following the stellar distribution density are highly disfavoured. We are also able to investigate the presence of an intermediate mass black hole in the centre of the cluster, showing that is not required by the available data, with an upper limit for the mass at $\sim 4000$ M$_{\odot}$., Comment: 14 pages, 10 figures, accepted for publication in MNRAS

Radio astronomy is a relatively young science: about an average human lifetime has passed since Karl Jansky’s measurement campaign took place at Holmdel, New Jersey, in the early 1930s, now celebrated as the birth date of radio astronomy. Most people working in the field today can claim to have personally known the pioneers in their countries. In the case of Italy, one such pioneer was Gianni Tofani, who passed away in February 2015. He fully devoted his professional life to scientific and technological research in astrophysics, mainly from the wonderful Arcetri hill of his beloved city, Florence. Furthermore, his management style was highly respected, bringing him to hold leadership positions such as the Director of the Institute of Radio Astronomy.

[Abridged] The Sardinia Radio Telescope (SRT) is the new 64-m dish operated by INAF (Italy). Its active surface will allow us to observe at frequencies of up to 116 GHz. At the moment, three receivers, one per focal position, have been installed and tested. The SRT was officially opened in October 2013, upon completion of its technical commissioning phase. In this paper, we provide an overview of the main science drivers for the SRT, describe the main outcomes from the scientific commissioning of the telescope, and discuss a set of observations demonstrating the SRT's scientific capabilities. One of the main objectives of scientific commissioning was the identification of deficiencies in the instrumentation and/or in the telescope sub-systems for further optimization. As a result, the overall telescope performance has been significantly improved. As part of the scientific commissioning activities, different observing modes were tested and validated, and first astronomical observations were carried out to demonstrate the science capabilities of the SRT. In addition, we developed astronomer-oriented software tools, to support future observers on-site. The astronomical validation activities were prioritized based on technical readiness and scientific impact. The highest priority was to make the SRT available for joint observations as part of European networks. As a result, the SRT started to participate (in shared-risk mode) in EVN (European VLBI Network) and LEAP (Large European Array for Pulsars) observing sessions in early 2014. The validation of single-dish operations for the suite of SRT first light receivers and backends continued in the following years, and was concluded with the first call for shared-risk/early-science observations issued at the end of 2015., 26 pages, 18 figures, accepted for publication in Section 13 'Astronomical instrumentation' of Astronomy & Astrophysics

This work is aimed at contributing to the effort of nations seeking to control international borders movement of dangerous chemical substances and nuclear material, in accordance with a multitude o...

Most of the X-ray binary systems containing neutron stars classified as Atoll sources show two different spectral states, called soft and hard. Moreover, a large number of these systems show a reflection component relativistically smeared in their spectra, which gives information on the innermost region of the system. Our aim is to investigate the poorly studied broadband spectrum of the low mass X-ray binary system 4U 1702-429, which was recently analysed combining XMM-Newton and INTEGRAL data. The peculiar value of the reflection fraction brought us to analyse further broadband spectra of 4U 1702-429. We re-analysed the spectrum of the XMM-Newton/INTEGRAL observation of 4U 1702-429 in the 0.3-60 keV energy range and we extracted three 0.1-100 keV spectra of the source analysing three observations collected with the BeppoSAX satellite. We find that the XMM-Newton/INTEGRAL spectrum is well fitted using a model composed of a disc blackbody plus a Comptonised component and a smeared reflection component. We used the same spectral model for the BeppoSAX spectra, finding out that the addition of a smeared reflection component is statistically significant. The best-fit values of the parameters are compatible to each other for the BeppoSAX spectra. We find that the reflection fraction is $0.05^{+0.03}_{-0.01}$ for the XMM-Newton/INTEGRAL spectrum and between 0.15 and 0.4 for BeppoSAX ones. The relative reflection fraction and the ionisation parameter are incompatible between the XMM-Newton/INTEGRAL and the BeppoSAX observations and the characteristics of the Comptonising corona suggest that the source was in a soft state in the former observation and in a hard state in the latter., 10 pages, 14 figures, submitted to A&A

We present a polarimetric analysis of 49 long-period pulsars discovered as part of the High Time Resolution Universe (HTRU) southern survey. The sources exhibit the typical characteristics of "old" pulsars, with low fractional linear and circular polarisation and narrow, multicomponent profiles. Although the position angle swings are generally complex, for two of the analysed pulsars (J1622-3751 and J1710-2616) we obtained an indication of the geometry via the rotating vector model. We were able to determine a value of the rotation measure (RM) for 34 of the sources which, when combined with their dispersion measures (DM), yields an integrated magnetic field strength along the line of sight. With the data presented here, the total number of values of RM associated to pulsars discovered during the HTRU southern survey sums to 51. The RMs are not consistent with the hypothesis of a counter-clockwise direction of the Galactic magnetic field within an annulus included between 4 and 6 kpc from the Galactic centre. A partial agreement with a counter-clockwise sense of the Galactic magnetic field within the spiral arms is however found in the area of the Carina-Sagittarius arm., 18 pages, 5 figures, 2 tables, accepted by MNRAS

We have used millisecond pulsars (MSPs) from the southern High Time Resolution Universe (HTRU) intermediate latitude survey area to simulate the distribution and total population of MSPs in the Galaxy. Our model makes use of the scale factor method, which estimates the ratio of the total number of MSPs in the Galaxy to the known sample. Using our best fit value for the z-height, z=500 pc, we find an underlying population of MSPs of 8.3(\pm 4.2)*10^4 sources down to a limiting luminosity of L_min=0.1 mJy kpc^2 and a luminosity distribution with a steep slope of d\log N/d\log L = -1.45(\pm 0.14). However, at the low end of the luminosity distribution, the uncertainties introduced by small number statistics are large. By omitting very low luminosity pulsars, we find a Galactic population above L_min=0.2 mJy kpc^2 of only 3.0(\pm 0.7)*10^4 MSPs. We have also simulated pulsars with periods shorter than any known MSP, and estimate the maximum number of sub-MSPs in the Galaxy to be 7.8(\pm 5.0)*10^4 pulsars at L=0.1 mJy kpc^2. In addition, we estimate that the high and low latitude parts of the southern HTRU survey will detect 68 and 42 MSPs respectively, including 78 new discoveries. Pulsar luminosity, and hence flux density, is an important input parameter in the model. Some of the published flux densities for the pulsars in our sample do not agree with the observed flux densities from our data set, and we have instead calculated average luminosities from archival data from the Parkes Telescope. We found many luminosities to be very different than their catalogue values, leading to very different population estimates. Large variations in flux density highlight the importance of including scintillation effects in MSP population studies., Comment: 13 pages, 5 figures, 5 tables. Accepted for publication in MNRAS

For the past couple of decades, the Parkes radio telescope has been regularly observing the millisecond pulsars in 47 Tucanae (47 Tuc). This long-term timing program was designed to address a wide range of scientific issues related to these pulsars and the globular cluster where they are located. In this paper, the first of a series, we address one of these objectives: the characterization of four previously known binary pulsars for which no precise orbital parameters were known, namely 47 Tuc P, V, W and X (pulsars 47 Tuc R and Y are discussed elsewhere). We determined the previously unknown orbital parameters of 47 Tuc V and X and greatly improved those of 47 Tuc P and W. For pulsars W and X we obtained, for the first time, full coherent timing solutions across the whole data span, which allowed a much more detailed characterization of these systems. 47 Tuc W, a well-known tight eclipsing binary pulsar, exhibits a large orbital period variability, as expected for a system of its class. 47 Tuc X turns out to be in a wide, extremely circular, 10.9-day long binary orbit and its position is ~3.8 arcmin away from the cluster center, more than three times the distance of any other pulsar in 47 Tuc. These characteristics make 47 Tuc X a very different object with respect to the other pulsars of the cluster., Comment: Accepted for publication by MNRAS, 18 pages, 11 figures

We present new observations of the galaxy cluster 3C 129 obtained with the Sardinia Radio Telescope in the frequency range 6000-7200 MHz, with the aim to image the large-angular-scale emission at high-frequency of the radio sources located in this cluster of galaxies. The data were acquired using the recently-commissioned ROACH2-based backend to produce full-Stokes image cubes of an area of 1 deg x 1 deg centered on the radio source 3C 129. We modeled and deconvolved the telescope beam pattern from the data. We also measured the instrumental polarization beam patterns to correct the polarization images for off-axis instrumental polarization. Total intensity images at an angular resolution of 2.9 arcmin were obtained for the tailed radio galaxy 3C 129 and for 13 more sources in the field, including 3C 129.1 at the galaxy cluster center. These data were used, in combination with literature data at lower frequencies, to derive the variation of the synchrotron spectrum of 3C 129 along the tail of the radio source. If the magnetic field is at the equipartition value, we showed that the lifetimes of radiating electrons result in a radiative age for 3C 129 of t_syn = 267 +/- 26 Myrs. Assuming a linear projected length of 488 kpc for the tail, we deduced that 3C 129 is moving supersonically with a Mach number of M=v_gal/c_s=1.47. Linearly polarized emission was clearly detected for both 3C 129 and 3C 129.1. The linear polarization measured for 3C 129 reaches levels as high as 70% in the faintest region of the source where the magnetic field is aligned with the direction of the tail., Comment: 19 pages, 17 figures, accepted for publication in MNRAS

In the early months of 2012 investigative works for the construction of a thermal pool within a small artificial basin in the district of Poggetti Vecchi, close to Grosseto unearthed a Palaeolithic site with many remains of Palaeoloxodon antiquus, alongside those of several other vertebrates. Urgent excavations were carried out by the Tuscan Archaeological Commission between March and June 2012 and in November 2013, over an area of around 160 m2 within the artificial basin. The work was complicated by the constant flow of hot spring water; the pool was emptied during the excavation by a large pump, while it was filled with water during the rest of the day. The same level yielded a set of wooden artefacts of great scientific relevance. These items are presently being restored and under study. Based on preliminary analytical results, the oldest level of frequentation of the Poggetti Vecchi site can be attributed to a time period ranging around 150- 200 ka BP. The area is located at the foot of a small limestone land surface elevation of around 11 metres above sea level, along the shore of an old lake, now filled. The area was repeatedly frequented by prehistoric man, probably at the beginning of the middle Palaeolithic, for hunting and/or scavenging carcasses of large herbivores that lived in the area. The research in the site of Poggetti Vecchi called for a multidisciplinary approach, involving numerous specialists from different scientific disciplines. The study, still in progress, will include also absolute dating, which is however difficult to obtain in presence of hot spring water.

In the period 2012 June - 2013 October, the Sardinia Radio Telescope (SRT) went through the technical commissioning phase. The characterization involved three first-light receivers, ranging in frequency between 300MHz and 26GHz, connected to a Total Power back-end. It also tested and employed the telescope active surface installed in the main reflector of the antenna. The instrument status and performance proved to be in good agreement with the expectations in terms of surface panels alignment (at present 300 um rms to be improved with microwave holography), gain (~0.6 K/Jy in the given frequency range), pointing accuracy (5 arcsec at 22 GHz) and overall single-dish operational capabilities. Unresolved issues include the commissioning of the receiver centered at 350 MHz, which was compromised by several radio frequency interferences, and a lower-than-expected aperture efficiency for the 22-GHz receiver when pointing at low elevations. Nevertheless, the SRT, at present completing its Astronomical Validation phase, is positively approaching its opening to the scientific community., Comment: 20 pages

We report on the pulse-to-pulse energy distributions and phase-resolved modulation properties for catalogued pulsars in the southern High Time Resolution Universe intermediate-latitude survey. We selected the 315 pulsars detected in a single-pulse search of this survey, allowing a large sample unbiased regarding any rotational parameters of neutron stars. We found that the energy distribution of many pulsars is well-described by a log-normal distribution, with few deviating from a small range in log-normal scale and location parameters. Some pulsars exhibited multiple energy states corresponding to mode changes, and implying that some observed "nulling" may actually be a mode-change effect. PSRJ1900-2600 was found to emit weakly in its previously-identified "null" state. We found evidence for another state-change effect in two pulsars, which show bimodality in their nulling time scales; that is, they switch between a continuous-emission state and a single-pulse-emitting state. Large modulation occurs in many pulsars across the full integrated profile, with increased sporadic bursts at leading and trailing sub-beam edges. Some of these high-energy outbursts may indicate the presence of "giant pulse" phenomena. We found no correlation with modulation and pulsar period, age, or other parameters. Finally, the deviation of integrated pulse energy from its average value was generally quite small, despite the significant phase-resolved modulation in some pulsars; we interpret this as tenuous evidence of energy regulation between distinct pulsar sub-beams.

Here we report on observations of the radio magnetar PSR J1622-4950 at frequencies from 1.4 to 17 GHz. We show that although its flux density is varying up to a factor of ~10 within a few days, it has on average decreased by a factor of 2 over the last 700 days. At the same time, timing analysis indicates a trend of decreasing spin-down rate over our entire data set, again of about a factor of 2 over 700 days, but also an erratic variability in the spin-down rate within this time span. Integrated pulse profiles are often close to 100 per cent linearly polarized, but large variations in both the profile shape and fractional polarization are regularly observed. Furthermore, the behaviour of the position angle of the linear polarization is very complex - offsets in both the absolute position angle and the phase of the position angle sweep are often seen and the occasional presence of orthogonal mode jumps further complicates the picture. However, model fitting indicates that the magnetic and rotation axes are close to aligned. Finally, a single pulse analysis has been carried out at four observing frequencies, demonstrating that the wide pulse profile is built up of narrow spikes of emission, with widths that scale inversely with observing frequency. All three of the known radio magnetars seem to have similar characteristics, with highly polarized emission, time-variable flux density and pulse profiles, and with spectral indices close to zero.

Millisecond pulsars are thought to be neutron stars that have been spun-up by accretion of matter from a binary companion. Although most are in binary systems, some 30% are solitary, and their origin is therefore mysterious. PSR J1719-1438, a 5.7 ms pulsar, was detected in a recent survey with the Parkes 64m radio telescope. We show that it is in a binary system with an orbital period of 2.2 h. Its companion's mass is near that of Jupiter, but its minimum density of 23 g cm$^{-3}$ suggests that it may be an ultra-low mass carbon white dwarf. This system may thus have once been an Ultra Compact Low-Mass X-ray Binary, where the companion narrowly avoided complete destruction., Comment: 16 pages, 3 figures. Science Express, in press

We present the discovery of 5 millisecond pulsars found in the mid-Galactic latitude portion of the High Time Resolution Universe (HTRU) Survey. The pulsars have rotational periods from ~2.3 to ~7.5 ms, and all are in binary systems with orbital periods ranging from ~0.3 to ~150 d. In four of these systems, the most likely companion is a white dwarf, with minimum masses of ~0.2 Solar Masses. The other pulsar, J1731-1847, has a very low mass companion and exhibits eclipses, and is thus a member of the "black widow" class of pulsar binaries. These eclipses have been observed in bands centred near frequencies of 700, 1400 and 3000 MHz, from which measurements have been made of the electron density in the eclipse region. These measurements have been used to examine some possible eclipse mechanisms. The eclipse and other properties of this source are used to perform a comparison with the other known eclipsing and "black widow" pulsars. These new discoveries occupy a short-period and high-dispersion measure (DM) region of parameter space, which we demonstrate is a direct consequence of the high time and frequency resolution of the HTRU survey. The large implied distances to our new discoveries makes observation of their companions unlikely with both current optical telescopes and the Fermi Gamma-ray Space Telescope. The extremely circular orbits make any advance of periastron measurements highly unlikely. No relativistic Shapiro delays are obvious in any of the systems, although the low flux densities would make their detection difficult unless the orbits were fortuitously edge-on.

We present the search methods and initial results for transient radio signals in the High Time Resolution Universe (HTRU) Survey. The HTRU survey's single-pulse search, the software designed to perform the search, and a determination of the HTRU survey's sensitivity to single pulses are described. Initial processing of a small fraction of the survey has produced 11 discoveries, all of which are sparsely-emitting neutron stars, as well as provided confirmation of two previously unconfirmed neutron stars. Most of the newly discovered objects lie in regions surveyed previously, indicating both the improved sensitivity of the HTRU survey observing system and the dynamic nature of the radio sky. The cycles of active and null states in nulling pulsars, rotating radio transients (RRATs), and long-term intermittent pulsars are explored in the context of determining the relationship between these populations, and of the sensitivity of a search to the various radio-intermittent neutron star populations. This analysis supports the case that many RRATs are in fact high-null-fraction pulsars (i. e. with null fraction >~0.95), and indicates that intermittent pulsars appear distinct from nulling pulsars in their activity cycle timescales. We find that in the measured population, there is a deficit of pulsars with typical emission timescales greater than ~300 s that is not readily explained by selection effects. The HTRU low-latitude survey will be capable of addressing whether this deficit is physical. We predict that the HTRU survey will explore pulsars with a broad range of nulling fractions (up to and beyond 0.999), and at its completion is likely to increase the currently known RRATs by a factor of more than two.

Direct detection of low-frequency gravitational waves ($10^{-9} - 10^{-8}$ Hz) is the main goal of pulsar timing array (PTA) projects. One of the main targets for the PTAs is to measure the stochastic background of gravitational waves (GWB) whose characteristic strain is expected to approximately follow a power-law of the form $h_c(f)=A (f/\hbox{yr}^{-1})^{\alpha}$, where $f$ is the gravitational-wave frequency. In this paper we use the current data from the European PTA to determine an upper limit on the GWB amplitude $A$ as a function of the unknown spectral slope $\alpha$ with a Bayesian algorithm, by modelling the GWB as a random Gaussian process. For the case $\alpha=-2/3$, which is expected if the GWB is produced by supermassive black-hole binaries, we obtain a 95% confidence upper limit on $A$ of $6\times 10^{-15}$, which is 1.8 times lower than the 95% confidence GWB limit obtained by the Parkes PTA in 2006. Our approach to the data analysis incorporates the multi-telescope nature of the European PTA and thus can serve as a useful template for future intercontinental PTA collaborations.

We have embarked on a survey for pulsars and fast transients using the 13-beam Multibeam receiver on the Parkes radio telescope. Installation of a digital backend allows us to record 400 MHz of bandwidth for each beam, split into 1024 channels and sampled every 64 us. Limits of the receiver package restrict us to a 340 MHz observing band centred at 1352 MHz. The factor of eight improvement in frequency resolution over previous multibeam surveys allows us to probe deeper into the Galactic plane for short duration signals such as the pulses from millisecond pulsars. We plan to survey the entire southern sky in 42641 pointings, split into low, mid and high Galactic latitude regions, with integration times of 4200, 540 and 270 s respectively. Simulations suggest that we will discover 400 pulsars, of which 75 will be millisecond pulsars. With ~30% of the mid-latitude survey complete, we have re-detected 223 previously known pulsars and discovered 27 pulsars, 5 of which are millisecond pulsars. The newly discovered millisecond pulsars tend to have larger dispersion measures than those discovered in previous surveys, as expected from the improved time and frequency resolution of our instrument.

We report on the optical identification of the companion to the eclipsing millisecond pulsar PSR J1701$-$3006B in the globular cluster NGC 6266. A relatively bright star with an anomalous red colour and an optical variability ($\sim$ 0.2 mag) that nicely correlates with the orbital period of the pulsar ($\sim$ 0.144 days) has been found nearly coincident with the pulsar nominal position. This star is also found to lie within the error box position of an X-ray source detected by Chandra observations, thus supporting the hypothesis that some interaction is occurring between the pulsar wind and the gas streaming off the companion. Although the shape of the optical light curve is suggestive of a tidally deformed star which has nearly completely filled its Roche lobe, the luminosity ($\sim 1.9 L_\odot$) and the surface temperature ($\sim 6000$ K) of the star, deduced from the observed magnitude and colours, would imply a stellar radius significantly larger than the Roche lobe radius. Possible explanations for this apparent inconsistency are discussed., 11 pages, 3 figures, ApJL accepted

We investigate the age constraints that can be placed on the double pulsar system using models for the spin-down of the first-born 22.7-ms pulsar A and the 2.77-s pulsar B with characteristic ages of 210 and 50 Myr respectively. Standard models assuming dipolar spin-down of both pulsars suggest that the time since the formation of B is ~50 Myr, i.e. close to B's characteristic age. However, adopting models which account for the impact of A's relativistic wind on B's spin-down we find that the formation of B took place either 80 or 180 Myr ago, depending the interaction mechanism. Formation 80 Myr ago, closer to B's characteristic age, would result in the contribution from J0737-3039 to the inferred coalescence rates for double neutron star binaries increasing by 40%. The 180 Myr age is closer to A's characteristic age and would be consistent with the most recent estimates of the coalescence rate. The new age constraints do not significantly impact recent estimates of the kick velocity, tilt angle between pre and post-supernova orbital planes or pre-supernova mass of B's progenitor., 5 pages, 2 figures, 1 table, accepted for publication by MNRAS

We present timing observations of four millisecond pulsars discovered in the Parkes 20-cm multibeam pulsar survey of the Galactic plane. PSRs J1552-4937 and J1843-1448 are isolated objects with spin periods of 6.28 and 5.47 ms respectively. PSR J1727-2946 is in a 40-day binary orbit and has a spin period of 27 ms. The 4.43-ms pulsar J1813-2621 is in a circular 8.16-day binary orbit around a low-mass companion star with a minimum companion mass of 0.2 solar masses. Combining these results with detections from five other Parkes multibeam surveys, gives a well-defined sample of 56 pulsars with spin periods below 20 ms. We develop a likelihood analysis to constrain the functional form which best describes the underlying distribution of spin periods for millisecond pulsars. The best results were obtained with a log-normal distribution. A gamma distribution is less favoured, but still compatible with the observations. Uniform, power-law and Gaussian distributions are found to be inconsistent with the data. Galactic millisecond pulsars being found by current surveys appear to be in agreement with a log-normal distribution which allows for the existence of pulsars with periods below 1.5 ms., Comment: 10 pages, 4 tables, 7 figures, accepted for publication in MNRAS

Aims: The binary pulsar PSR J1811-1736 has been identified, since its discovery, as a member of a double neutron star system. Observations of such binary pulsars allow the measurement of general relativistic effects, which in turn lead to information about the orbiting objects and, in a few cases, to tests of theories of gravity. Methods: Regular timing observations have been carried out with three of the largest European radio telescopes involved in pulsar research. The prospects of continued observations were studied with simulated timing data. Pulse scattering times were measured using dedicated observations at 1.4 GHz and at 3.1 GHz, and the corresponding spectral index has also been determined. The possibility of detecting the yet unseen companion as a radio pulsar was also investigated. A study of the natal kick received by the younger neutron star at birth was performed. Results: We present an up to date and improved timing solution for the binary pulsar PSR J1811-1736. One post-Keplerian parameter, the relativistic periastron advance, is measured and leads to the determination of the total mass of this binary system. The pulse profile at 1.4 GHz is heavily broadened by interstellar scattering, limiting the timing precision achievable at this frequency and the measurability of other post-keplerian parameters. Interstellar scattering is unlikely to be the reason for the continued failure to detect radio pulsations from the companion of PSR J1811-1736. The probability distribution that we derive for the amplitude of the kick imparted on the companion neutron star at its birth indicates that the kick has been of low amplitude., 8 pages, 4 figures, accepted for publication on A&A. (Abridged abstract)

The double pulsar system, PSR J0737-3039A/B, is unique in that both neutron stars are detectable as radio pulsars. This, combined with significantly higher mean orbital velocities and accelerations when compared to other binary pulsars, suggested that the system would become the best available testbed for general relativity and alternative theories of gravity in the strong-field regime. Here we report on precision timing observations taken over the 2.5 years since its discovery and present four independent strong-field tests of general relativity. Use of the theory-independent mass ratio of the two stars makes these tests uniquely different from earlier studies. By measuring relativistic corrections to the Keplerian description of the orbital motion, we find that the ``post-Keplerian'' parameter s agrees with the value predicted by Einstein's theory of general relativity within an uncertainty of 0.05%, the most precise test yet obtained. We also show that the transverse velocity of the system's center of mass is extremely small. Combined with the system's location near the Sun, this result suggests that future tests of gravitational theories with the double pulsar will supersede the best current Solar-system tests. It also implies that the second-born pulsar may have formed differently to the usually assumed core-collapse of a helium star., Appeared in Science Express, Sept. 14, 2006. Includes supporting material

The timing of radio pulsars provides a tool for studying a wide range of fundamental physical and astrophysical problems. The best results are obtained by regular, frequent timing observations of a large number of sources at various frequencies. We describe a project which aims to improve on all of the above parameters by combining timing data from the pulsar timing programs of 4 European groups to form the so-called EPTA. These data will be used to study, amongst other things, relativistic binaries and gravitational waves.

The Parkes High-Latitude pulsar survey covers a region of the sky enclosed by Galactic longitudes 220° < l < 260° and Galactic latitudes |b| < 60°. The observations have been performed using the 20-cm multibeam receiver on the Parkes 64-m radio telescope. A total of 6456 point-ings of 265 s each have been collected. The system adopted provided a sensitivity limit, for long-period pulsars with 5 per cent duty cycles, of ∼0.5 mJy. Data analysis resulted in the detection of 42 pulsars of which 18 were new discoveries. Four of these belong to the class of the millisecond - or recycled - pulsars; three of these four are in binary systems. The double pulsar system J0737-3039 is among those and has been presented elsewhere. Here, we discuss the other discoveries and provide timing parameters for the objects for which we have a phase-connected solution.

We report the discovery of PSR J1906+0746, a young 144-ms pulsar in a highly relativistic 3.98-hr orbit with an eccentricity of 0.085 and expected gravitational wave coalescence time of 300 Myr. The new pulsar was found during precursor survey observations with the Arecibo 1.4-GHz feed array system and retrospectively detected in the Parkes Multibeam plane pulsar survey data. From radio follow-up observations with Arecibo, Jodrell Bank, Green Bank, and Parkes, we have measured the spin-down and binary parameters of the pulsar and its basic spectral and polarization properties. We also present evidence for pulse profile evolution, which is likely due to geodetic precession, a relativistic effect caused by the misalignment of the pulsar spin and total angular momentum vectors. Our measurements show that PSR J1906+0746 is a young object with a characteristic age of 112 kyr. From the measured rate of orbital periastron advance 7.57+/-0.03 deg/yr, we infer a total system mass of 2.61+/-0.02 Msun. While these parameters suggest that the PSR J1906+0746 binary system might be a younger version of the double pulsar system, intensive searches for radio pulses from the companion have so far been unsuccessful. It is therefore not known whether the companion is another neutron star or a massive white dwarf. Regardless of the nature of the companion, a simple calculation suggests that the Galactic birth rate of binaries similar to PSR J1906+0746 is 60/Myr. This implies that PSR J1906+0746 will make a significant contribution to the computed cosmic inspiral rate of compact binary systems.

SETI-Italia is the Italian national SETI Program. It is run as a part of the Italian Bioastronomy Project, by IRA (Istituto di Radioastronomia) a subsidiary of the Istituto Nazionale di Astrofisica. At the moment, Italy is the only European country conducting a continuous SETI “listening” program, while occasional SETI searches may be conducted in France and the Ukraine. Outside Europe, continuous SETI programs are conducted in the USA, Australia and Argentina. The SETI-Italia activities started early in 1998 with a Serendip IV system connected in piggy-back mode to the Medicina VLBI 32-m antenna dish. In the years 2002/2003, considerable efforts were devoted to set up a fast computational system capable of computing the KLT (Karhunen–Loeve transform). This is a virtually new mathematical procedure in SETI, and is much more general than the FFT. It can search for the presence of signals both narrow-band and wide-band embedded in noise both coloured and white. The mathematical features the KLT have already been described in this Conference by Claudio Maccone [Innovative SETI by the KLT, Pesek Lecture 2003, paper # IAA.9.1.01 presented in Bremen, in this 2003 International Astronautical Congress [1] ; Telecommunications, KLT and Relativity, vol. 1, IPI Press, Colorado Springs, CO, 1994, ISBN #1-880930-04-8 [2] .]. Our software implementation of the KLT follows these lines and also is indebted to some basic ideas put forward by Robert S. Dixon [On the detection of unknown signals, in: G. Seth Shostak (Ed.), Third Decennial US–USSR Conference on SETI, Astronomical Society of the Pacific Conference Series, vol. 47, 1993, pp. 128–140] of the Ohio State Radio Observatory as early as 1993.

This first-ever double-pulsar system was discovered in 2003, consisting of two visible pulsars which orbit the common centre of mass in a slightly eccentric orbit in only 2.4 hours. One of the pulsars appears to be old and recycled with a spin period of only 22 ms. Its companion is younger and rotating slower with a period of 2.8 s, confirming the long-proposed recycling theory for millisecond pulsars. The system provides an exciting opportunity to study the workings of pulsar magnetospheres and represents a truly unique laboratory for relativistic gravitational physics. Both aspects are greatly facilitated by the very fortunate fact that the orbit is seen nearly perfectly edge on. This alignment allows the detection of the Shapiro delay in the pulse arrival times of the 22-ms pulsar when its pulses propagate through the curved space-time near the slowly rotating pulsar. In addition to updating on the continuing observations, this contribution introduces a new timing model that represents a variation of Damour-Deruelle (1986) for highly inclined orbits. (c) 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

We report the discovery of three binary millisecond pulsars during the Parkes Multibeam Pulsar Survey of the Galactic Plane. The objects are highly recycled and are in orbits of many tens of days about low-mass white-dwarf companions. The eccentricity of one object, PSR J1853+1303, is more than an order of magnitude lower than predicted by the theory of convective fluctuations during tidal circularization. We demonstrate that, under the assumption that the systems are randomly oriented, current theoretical models of the core-mass--orbital-period relation for the progenitors of these systems likely overestimate the white-dwarf masses, strengthening previous concerns about the match of these models to the data. The new objects allow us to update the limits on violation of relativistic equivalence principles to 95% confidence upper limits of 5.6 x 10^-3 for the Strong Equivalence Principle parameter Delta and 4.0 x 10^-20 for the Lorentz-invariance/momentum-conservation parameter alpha_3., 8 pages; accepted to ApJ. Small changes to parameters from including correct Parkes clock corrections; changes/corrections to Figures 1, 4 and 5; minor changes to text and references. The limit on Delta has increased from 0.0055 to 0.0056